CN102727934B - Manufacturing method and application of inductive artificial periosteum - Google Patents
Manufacturing method and application of inductive artificial periosteum Download PDFInfo
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- CN102727934B CN102727934B CN201210204048.5A CN201210204048A CN102727934B CN 102727934 B CN102727934 B CN 102727934B CN 201210204048 A CN201210204048 A CN 201210204048A CN 102727934 B CN102727934 B CN 102727934B
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- osteocomma
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Abstract
The invention discloses a manufacturing method of an inductive artificial periosteum. The method comprises the steps that: animal bone epiphysis is adopted, and is cut into bone slices with thicknesses of 0.5-1mm; the bone slices are decalcified in a hydrochloric acid solution, until the bone slices turn soft; the softened bone slices are cleaned by washing, and are placed in an oxidation liquid; under a baking environment, foreign protein antigen is extracted; the bone slices with the foreign protein antigen extracted are cleaned by washing, and are defatted in a chloroform-methanol liquid; the defatted bone slices are cleaned by washing, sufficiently volatilized, and cleaned by using clear water; the cleaned bone slices are extracted again in the oxidation liquid; the extracted bone slices are cleaned by using clear water, and are cleaned by rinsing by using purified water; the bone slices are trimmed, and residual soft tissues on periosteum are removed; the periosteum is shaped, and periosteum slices are manufactured. The invention also discloses an application of the inductive artificial periosteum. The periosteum slice provided by the invention can be directly applied on a fracture line for achieving a fracture healing promoting purpose.
Description
Technical field
The present invention relates to artificial periosteum technology, particularly relate to a kind of manufacture method and application thereof of inductivity artificial periosteum.
Background technology
In prior art, transplant promotion union of fracture field at artificial bone substitute materials and bone, no matter be to be all stripe shape, grain type or powdery by inorganic or prepared by animal bone material, application mode is all that implantation or filling bone are damaged, do not have chaffy form, be not more directly attached to the application mode that reaches promotion union of fracture on fracture line.
Summary of the invention
Based on this, for the problems referred to above, be necessary to propose a kind of manufacture method and application thereof of inductivity artificial periosteum.
Technical scheme of the present invention is: a kind of manufacture method of inductivity artificial periosteum, comprises the following steps:
Get animal bone epiphysis, cut into the osteocomma that 0.5mm-1mm is thick;
Osteocomma puts into the decalcification of hydrochloric acid liquid until the complete deliquescing of osteocomma;
Clean the osteocomma after deliquescing, put into oxidation liquid, in curing environment, extract foreign protein antigen;
Clean the osteocomma that extracted foreign protein antigen, put into the defat of chloroform-methanol liquid;
Clean the osteocomma after defat, fully after volatilization, clean with clear water again;
Osteocomma after clear water is cleaned, puts into oxidation liquid extracting again;
Clear water is cleaned the osteocomma after extracting, then cleans osteocomma in order to pure water rinsing;
Prune and remove the soft tissue that periosteum is remaining;
Periosteum molding, makes diaphragm.
This manufacture method is spent the night, extracting, pure water rinsing, is pruned away the multiple operation except the remaining soft tissue of periosteum and periosteum molding film-making by section, decalcification, extraction foreign protein antigen, defat, volatilization, make periosteum diaphragm, can directly be attached to and on fracture line, reach the effect that promotes union of fracture, every one procedure step that is all absolutely necessary.
Therein in an embodiment, in being the baking box of 35 DEG C, temperature extracts foreign protein antigen 7 hours.Object is to guarantee fully to extract foreign protein antigen.
Therein in an embodiment, clean the osteocomma that extracted foreign protein antigen, put into the chloroform-methanol liquid defat 24 hours of 1:1.Object is the effect that guarantees fully to reach defat.
Therein in an embodiment, in oxidation liquid, contain weight ratio and be 20% H
20
2.Object is to ensure H in oxidation liquid
20
2content moderate.
In an embodiment, the osteocomma after clear water is cleaned, puts into oxidation liquid extracting 24 hours again therein.Object is that the effect that ensures extracting reaches best.
In an embodiment, osteocomma is put into the decalcification of 0.6mol hydrochloric acid liquid therein.Object is that the effect that ensures decalcification reaches optimum state.
In an embodiment, further comprising the steps of therein: after getting animal bone epiphysis, to remove remaining muscle, periosteum, articular cartilage and the cortical bone of epiphysis surrounding, epiphysis is cut into square spongy bone piece, then be cut to osteocomma.Object is to ensure that the osteocomma that cuts into affects the various more than material of manufacturing effect without remaining.
In an embodiment, further comprising the steps of therein: to the diaphragm lyophilization of making, oxirane disinfection sterilization.Object is to ensure that the diaphragm of making can reach best storage condition.
Inductivity artificial periosteum of the present invention is as the fracture for the treatment of orthopaedics, thoracic surgery and neurosurgery.
Orthopaedics: diaphragm is put into physiological saline solution and soaked and treat for 3-5 minute its deliquescing diaphragm to be attached on fracture line after all kinds of fracture operative reductions, and available steel plate is pushed down or fixed with suture.
Thoracic surgery: fracture of rib is attached to fracture line place by diaphragm after resetting, and then in using, setting tool is fixed.
Neurosurgery: fracture at the bottom of skull, available diaphragm is attached to fracture line place.
The invention has the beneficial effects as follows: whole manufacturing process is perfect, the diaphragm of making has been filled up in prior art the blank of diaphragm in inductivity artificial periosteum, and the diaphragm of making can directly be attached to and on fracture line, reach the object that promotes union of fracture.
Brief description of the drawings
Fig. 1 is the process chart of the embodiment of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, embodiments of the invention are elaborated.
Embodiment:
As shown in Figure 1, a kind of manufacture method of inductivity artificial periosteum, comprises the following steps:
Step S101, gets animal bone epiphysis, removes remaining muscle, periosteum, articular cartilage and the cortical bone of epiphysis surrounding, epiphysis is cut into square spongy bone piece, then be cut to the osteocomma that 0.5mm-1mm is thick, and the thickness of osteocomma is preferably 1mm.
Step S102, osteocomma puts into the liquid decalcification of 0.6mo l hydrochloric acid until the complete deliquescing of osteocomma.
Step S103, cleans the osteocomma after deliquescing, puts into the H that contains weight 20%
20
2in oxidation liquid, in temperature is the baking box of 35 DEG C, extract foreign protein antigen 7 hours, can guarantee fully to extract foreign protein antigen.
Step S104, cleans and extracted the osteocomma of foreign protein antigen, puts into the chloroform-methanol liquid defat 24 hours of 1:1, can guarantee fully to reach the effect of defat.
Step S105, cleans the osteocomma after defat, volatilizees 8 hours-24 hours, then cleans with clear water, and the time of volatilization preferably can volatilize a whole night, within second day, cleans with clear water again.
Step S106, the osteocomma after clear water is cleaned, puts into the H that contains weight 20% again
20
2extracting 24 hours in oxidation liquid.
Step S107, clear water is cleaned the osteocomma after extracting, then cleans osteocomma in order to pure water rinsing.
Step S108, prunes and removes the soft tissue that periosteum is remaining.
Step S109, periosteum molding, makes diaphragm.
Step S110, to the diaphragm lyophilization of making, oxirane disinfection sterilization.
Manufacture method described in above-described embodiment is spent the night, extracting, pure water rinsing, is pruned away the multiple operation except the remaining soft tissue of periosteum and periosteum molding film-making by section, decalcification, extraction foreign protein antigen, defat, volatilization, make periosteum diaphragm, can directly be attached to the effect that reaches promotion union of fracture on fracture line.
Inductivity artificial periosteum of the present invention is as the fracture for the treatment of orthopaedics, thoracic surgery and neurosurgery.Instantiation is:
Orthopaedics: diaphragm is put into physiological saline solution and soaked and treat for 3-5 minute its deliquescing diaphragm to be attached on fracture line after all kinds of fracture operative reductions, and available steel plate is pushed down or fixed with suture.
Thoracic surgery: fracture of rib is attached to fracture line place by diaphragm after resetting, and then in using, setting tool is fixed.
Neurosurgery: fracture at the bottom of skull, available diaphragm is attached to fracture line place.
The above embodiment has only expressed the specific embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.
Claims (1)
1. a manufacture method for inductivity artificial periosteum, is characterized in that, comprises the following steps:
Get animal bone epiphysis, after removing remaining muscle, periosteum, articular cartilage and the cortical bone of epiphysis surrounding, epiphysis is cut into square spongy bone piece, then be cut to the osteocomma that 0.5mm-1mm is thick;
Osteocomma puts into the liquid decalcification of 0.6mol hydrochloric acid until the complete deliquescing of osteocomma;
Clean the osteocomma after deliquescing, put into oxidation liquid, in temperature is the baking box of 35 DEG C, extract foreign protein antigen 7 hours, in oxidation liquid, contain weight ratio and be 20% H
2o
2;
Clean and extracted the osteocomma of foreign protein antigen, put into the chloroform-methanol liquid defat 24 hours of 1:1;
Clean the osteocomma after defat, fully after volatilization, clean with clear water again;
Osteocomma after clear water is cleaned, puts into oxidation liquid extracting 24 hours again, contains weight ratio and be 20% H in oxidation liquid
2o
2;
Clear water is cleaned the osteocomma after extracting, then cleans osteocomma in order to pure water rinsing;
Prune and remove the soft tissue that periosteum is remaining;
Periosteum molding, makes diaphragm, to the diaphragm lyophilization of making, and oxirane disinfection sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210204048.5A CN102727934B (en) | 2012-06-20 | 2012-06-20 | Manufacturing method and application of inductive artificial periosteum |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210204048.5A CN102727934B (en) | 2012-06-20 | 2012-06-20 | Manufacturing method and application of inductive artificial periosteum |
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| Publication Number | Publication Date |
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| CN102727934A CN102727934A (en) | 2012-10-17 |
| CN102727934B true CN102727934B (en) | 2014-10-22 |
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| CN201210204048.5A Active CN102727934B (en) | 2012-06-20 | 2012-06-20 | Manufacturing method and application of inductive artificial periosteum |
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| CN104524635B (en) * | 2014-12-29 | 2017-03-15 | 上海骁博科技发展有限公司 | A kind of preparation method of xenogenesis spongy bone induced calvarial stopple |
Citations (6)
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|---|---|---|---|---|
| CN1101573A (en) * | 1993-10-13 | 1995-04-19 | 胡晓波 | Bone inducer |
| CN1273130A (en) * | 1999-12-22 | 2000-11-15 | 胡晓波 | Process for preparing material as substitute of human bone and able to promote growth of bone |
| CN1579562A (en) * | 2003-08-01 | 2005-02-16 | 中国人民解放军第四军医大学口腔医学院 | Preparation method for ceramic agg egate of same kind and different structure |
| EP1380311B1 (en) * | 2002-07-10 | 2005-03-09 | Jakob, Karl, Dipl.-Ing. Dr. h.c. | Multi-step process for making a bone regeneration paste |
| CN1903382A (en) * | 2005-07-29 | 2007-01-31 | 芮钢 | Method for preparing heterogenic bone with cell-removing matrix |
| CN101032631A (en) * | 2007-04-06 | 2007-09-12 | 四川大学华西医院 | Preparing method of heterotransplantation of bones |
-
2012
- 2012-06-20 CN CN201210204048.5A patent/CN102727934B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101573A (en) * | 1993-10-13 | 1995-04-19 | 胡晓波 | Bone inducer |
| CN1273130A (en) * | 1999-12-22 | 2000-11-15 | 胡晓波 | Process for preparing material as substitute of human bone and able to promote growth of bone |
| EP1380311B1 (en) * | 2002-07-10 | 2005-03-09 | Jakob, Karl, Dipl.-Ing. Dr. h.c. | Multi-step process for making a bone regeneration paste |
| CN1579562A (en) * | 2003-08-01 | 2005-02-16 | 中国人民解放军第四军医大学口腔医学院 | Preparation method for ceramic agg egate of same kind and different structure |
| CN1903382A (en) * | 2005-07-29 | 2007-01-31 | 芮钢 | Method for preparing heterogenic bone with cell-removing matrix |
| CN101032631A (en) * | 2007-04-06 | 2007-09-12 | 四川大学华西医院 | Preparing method of heterotransplantation of bones |
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| CN102727934A (en) | 2012-10-17 |
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