CN102726596B - Feather protein peptide feed additive and application thereof - Google Patents
Feather protein peptide feed additive and application thereof Download PDFInfo
- Publication number
- CN102726596B CN102726596B CN201210201404.8A CN201210201404A CN102726596B CN 102726596 B CN102726596 B CN 102726596B CN 201210201404 A CN201210201404 A CN 201210201404A CN 102726596 B CN102726596 B CN 102726596B
- Authority
- CN
- China
- Prior art keywords
- culture medium
- protein peptide
- state fermentation
- feather
- solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Fodder In General (AREA)
Abstract
The present invention discloses a feather protein peptide feed additive and an application thereof. The preparation method for the feather protein peptide feed additive comprises: 1, mixing a substrate and water to obtain a solid state fermentation culture medium A, inoculating saccharomyces cerevisiae and saccharomycopsis fibuligera in the solid state fermentation culture medium A, culturing for 28-30 hours at a temperature of 30 DEG C, and drying the resulting culture at a temperature of 35-45 DEG C after culturing to obtain a yeast culture; and 2, mixing avian feather powder and the yeast culture, adding water to the resulting mixed material, uniformly stirring to obtain a solid state fermentation culture medium B, respectively inoculating bnfillus licheniformis and bacillus subtilis in the solid state fermentation culture medium B, and carrying out fermentation culture for 30-45 hours at a temperature of 35-38 DEG C to obtain the feather protein peptide feed additive. The feather protein peptide feed additive of the present invention has the following advantages that: feather protein digestion absorption efficiency can be 90%; the additive contains a variety of bioactive peptides, and provides digestion and growth promotion effects; the additive has natural fermentation flavor and good palatability; and pica of livestock and poultry can be significantly improved.
Description
(1) technical field
The present invention relates to a kind of feather protein peptide feed addictive and preparation and application thereof.
(2) background technology
In recent years, with the intensive develop rapidly that turns to feature whole world aquaculture, for huge contribution has been made in mankind's growth in the living standard, but feed protein resource starts to occur serious shortage, has restricted the sustainable development of aquaculture.Yet being rich in the multiple essential amino acid of animal, protein content reaches 75%～90% poultry feather and is not but effectively utilized, the feather that China can collect utilization every year reaches 20～300,000 tons, after processing for 20,000 tons of the deficiencies of feed, most of feather and animal hair are not used appropriately, both waste resource, polluted again environment.The unavailable main cause of feather is that feather meal process technology does not pass a test, and product digestibility is low, and quality is uneven, causes feeding effect not good.
Utilize feather keratoprotein, must carry out science processing to keratin, crack the space structure of keratoprotein, make it become the state that can digest and assimilate.The processing of feather meal has been had to certain research both at home and abroad.At present, mainly adopt the processing of method to feather meal such as high-temperature high-pressure hydrolysis, acid-hydrolysis method, alkali hydrolysis method, enzymatic isolation method, microbial method and extrusion both at home and abroad.These methods can improve the digestibility of feather meal to a certain extent, but all exist obvious deficiency, as process technology does not pass a test, product digestibility is low, and quality is uneven, causes feeding effect not good, economic benefit is not good, and processing procedure causes secondary pollution etc.
(3) summary of the invention
The object of the invention is to provide a kind of feather protein peptide feed addictive and application, adopt many strain combined fermentations to produce a kind of feather protein peptide feed addictive on feather and yeast culture, not only having eliminated feather can not be by livestock and poultry digestibility and utilization problem, and contain various active peptide, keratinase, digestive ferment and somatomedin, the animal protein feeds such as the complete Peru Fish Dietary of energy, SDPP.
The technical solution used in the present invention is:
A kind of feather protein peptide feed addictive, described feather protein peptide feed addictive is prepared as follows: the preparation of (1) yeast culture: substrate is mixed with water a, obtain solid-state fermentation culture medium A, again saccharomyces cerevisiae and saccharomycopsis fibuligera are inoculated in solid-state fermentation culture medium A, at 30 ℃, cultivate 28～30h, after cultivation finishes by culture in 35～45 ℃ dry, obtain yeast culture; Described substrate is comprised of the raw material of following quality proportioning: wheat bran 40～60%, corn flour 3～10%, vinasse 15～20%, bean cake powder 20～25% and urea 2～5%; The ratio of described water a and substrate gross mass is 0.5～1:1; (2) feather protein peptide feed addictive preparation: poultry feather is pulverized, obtain poultry feather powder, poultry feather powder is mixed with the described yeast culture of step (1), add water b, stir, obtain solid-state fermentation culture medium B, respectively bacillus licheniformis and bacillus subtilis are seeded in solid-state fermentation culture medium B to 35～38 ℃ of fermented and cultured 30～45h, after fermented and cultured finishes, tunning be dry, pulverize, obtain described feather protein peptide feed addictive; The mass ratio that feeds intake of described poultry feather powder and yeast culture is 9～49:1, and the ratio of the addition of described water b and yeast culture and poultry feather powder gross mass is 0.5～1.2:1.
Further, the described saccharomyces cerevisiae of step (1) and saccharomycopsis fibuligera add with the form of mycetocyte suspension respectively.
Further, the addition of the described saccharomyces cerevisiae mycetocyte of step (1) suspension counts 1.2～3.6 * 10 with viable count
7cfu/g solid-state fermentation culture medium A dry weight.
Further, the addition of described saccharomycopsis fibuligera mycetocyte suspension counts 2～5 * 10 with viable count
7cfu/g solid-state fermentation culture medium A dry weight.
Further, the described poultry feather fineness of powder of step (2) is 20～40 orders, and moisture is less than 10%.
Further, the described bacillus licheniformis of step (2) and bacillus subtilis add with the form of mycetocyte suspension respectively.
Further, the addition of described bacillus licheniformis mycetocyte suspension counts 2～5 * 10 with viable count
7cfu/g solid-state fermentation culture medium B dry weight.
Further, the addition of described bacillus subtilis mycetocyte suspension counts 5～30 * 10 with viable count
7cfu/g solid-state fermentation culture medium B dry weight.
Further, described feather protein peptide feed addictive is prepared as follows: the preparation of (1) yeast culture: substrate is mixed with water a, under 0.12Mpa pressure, boiling 35min carries out sterilizing, shelving 35min again, obtain solid-state fermentation culture medium A, again saccharomyces cerevisiae ACCC20042 mycetocyte suspension and saccharomycopsis fibuligera ACCC20015 mycetocyte suspension are inoculated into respectively in solid-state fermentation culture medium A, at 30 ℃, cultivate 28～30h, after cultivation finishes, culture is dry at 35～45 ℃, obtain yeast culture, described substrate is comprised of the raw material of following quality proportioning: wheat bran 40～60%, corn flour 3～10%, vinasse 15～20%, bean cake powder 20～25% and urea 2～5%, the ratio of described water a and substrate gross mass is 0.65～1:1, the addition of described saccharomyces cerevisiae ACCC20042 mycetocyte suspension counts 1.2 * 10 with viable count
7cfu/g solid-state fermentation culture medium A dry weight, the addition of described saccharomycopsis fibuligera ACCC20015 mycetocyte suspension counts 2 * 10 with viable count
7cfu/g solid-state fermentation culture medium A dry weight, (2) preparation of feather protein peptide feed addictive: poultry feather powder is mixed with mass ratio 11.5～49:1 with the described yeast culture of step (1), add water b, stir, with cobalt-60 radiation sterilization under 5kGy, obtain solid-state fermentation culture medium B, respectively by bacillus licheniformis ACCC01198 mycetocyte suspension and bacillus subtilis ACCC01185 mycetocyte suspension inoculation to solid-state fermentation culture medium B, 35～38 ℃ of fermented and cultured 30～45h, after fermented and cultured finishes, tunning is dried to moisture at 45 ℃ and is less than 10%, being crushed to fineness is 40～60 orders, obtain described feather protein peptide feed addictive, described poultry feather powder moisture is less than 10%, and fineness is 20～40 orders, the ratio of described water b and yeast culture and poultry feather powder gross mass is 0.5～1.2:1, the addition of described bacillus licheniformis mycetocyte suspension counts 2～5 * 10 with viable count
7cfu/g solid-state fermentation culture medium B dry weight, the addition of described bacillus subtilis mycetocyte suspension counts 5～8 * 10 with viable count
7cfu/g solid-state fermentation culture medium B dry weight.
The application of described feather protein peptide feed addictive in feed for livestock, further, the effective mass addition of described feather protein peptide feed addictive in feed for livestock is 2～8%(weight).
Solid-state fermentation culture medium dry weight of the present invention refers to dries the solid-state fermentation culture medium of preparation to constant weight at 55～58 ℃, measures the water content of the standby solid-state fermentation culture medium of every restraint, calculates the dry weight of prepared solid-state fermentation culture medium according to water content.
Poultry feather powder of the present invention comes from the feather of various birds, preferably the feather of following bird: chicken, duck, goose or quail etc.
Saccharomyces cerevisiae of the present invention, be preferably saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC20042), Chinese agriculture microorganism fungus kind preservation administrative center provides, described saccharomyces cerevisiae ACCC20042 mycetocyte suspension is prepared as follows: saccharomyces cerevisiae ACCC20042 is seeded in maltose liquid glucose a seed culture medium (7～8 ° of Be of pol), 30 ℃ of heat insulating culture 10h, be forwarded to again in maltose liquid glucose b seed culture medium (7～8 ° of Be of pol), 30 ℃ of heat insulating culture 10h, then be forwarded in syrup culture medium (7～8 ° of Be of pol), cultivate 10h for 30 ℃, finally be forwarded to and expand 30 ℃ of heat insulating culture 20h of culture medium (consisting of: the mass ratio of wheat bran and flour and water is 1:3:20), obtain saccharomyces cerevisiae seed liquor, be saccharomyces cerevisiae mycetocyte suspension, the volume ratio of described maltose liquid glucose a and maltose liquid glucose b is 1:6, and described maltose liquid glucose a and maltose liquid glucose b are maltose liquid glucose, and letter itself does not have implication, flow process is: the former bacterium test tube of saccharomyces cerevisiae---little triangular flask seed (maltose liquid glucose 100ml, 7～8 ° of Be of pol) 30 ℃ of heat insulating culture 10h---proceed to large triangular flask (maltose liquid glucose 600ml, 7～8 ° of Be of pol), 30 ℃ of heat insulating culture 10h---proceed to (syrup 13kg in Ka Shi tank, 7～8 ° of Be expansion cultivations of pol) 10h---Ka Shi tank yeast starter is spread cultivation at distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 20h, is saccharomyces cerevisiae seed liquor.
Saccharomycopsis fibuligera of the present invention, is preferably saccharomycopsis fibuligera (Endomycopsisfibuligera ACCC20015), and by the preservation of Chinese agriculture microorganism fungus kind, administrative center provides.Described saccharomycopsis fibuligera ACCC20015 mycetocyte suspension preparation method is identical with described saccharomyces cerevisiae ACCC20042 mycetocyte suspension preparation method, flow process is: the former bacterium test tube of saccharomycopsis fibuligera---little triangular flask seed (maltose liquid glucose 100ml, 7～8 ° of Be of pol) 30 ℃ of heat insulating culture 10h---proceed to large triangular flask (maltose liquid glucose 600ml, 7～8 ° of Be of pol), 30 ℃ of heat insulating culture 10h---proceed to (syrup 13kg in Ka Shi tank, 7～8 ° of Be expansion cultivations of pol) 10h---Ka Shi tank yeast starter is spread cultivation at distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 20h, be saccharomycopsis fibuligera seed liquor.
Bacillus licheniformis of the present invention (Bacillus licheniformis), preferred bacillus licheniformis ACCC01198(Bacillus licheniformis) by Chinese agriculture microorganism fungus kind preservation administrative center, provided, described bacillus licheniformis mycetocyte suspension is prepared as follows: bacillus licheniformis is seeded in the liquid seed culture medium that is applicable to bacillus licheniformis growth, then put on shaking table, rotating speed is 240r/min, 40 ℃ of shaken cultivation 14h, obtain bacillus licheniformis seed liquor, be bacillus licheniformis mycetocyte suspension; Further, described bacillus licheniformis ACCC01198 mycetocyte suspension is prepared as follows: (1) inclined-plane is cultivated: slant medium final concentration consists of: beef extract 5g/L, peptone 10g/L, sodium chloride 5g/L, agar 30g/L, pH value 7.4, solvent is water; Bacillus licheniformis ACCC01198 is transferred in fresh test tube slant from the inclined-plane of preserving, in 37 ℃ of cultivation 18h, obtain slant strains; (2) seed culture: liquid seed culture medium final concentration consists of: beef extract 5g/L, soy peptone 10g/L, NaCl5g/L, pH7.0, solvent is water; From the cultured inclined-plane of step (1), with oese, choosing 1 ring is inoculated in the 250ml triangular flask that fills 50ml sterile liquid seed culture medium, then put 37 ℃ of vibrations (rotating speed is 120r/min) in swinging constant temperature speed governing shaking flask cabinet and cultivate 24h, obtain bacillus licheniformis ACCC01198 seed liquor, i.e. bacillus licheniformis ACCC01198 mycetocyte suspension.
Bacillus subtilis of the present invention, is preferably bacillus subtilis ACCC01185(Bacillus subtilis) by Chinese agriculture microorganism fungus kind preservation administrative center, provided.Described bacillus subtilis mycetocyte suspension is prepared as follows: bacillus subtilis is seeded in the liquid seed culture medium that is applicable to bacillus subtilis bacteria growing, 35～37 ℃, 220r/min shaking table are cultivated 24～26 hours, obtain bacillus subtilis seed liquor, i.e. bacillus subtilis mycetocyte suspension; Further, described bacillus subtilis ACCC01185 mycetocyte suspension is prepared as follows: (1) inclined-plane is cultivated: slant medium final concentration consists of: peptone 10g/L, beef extract 3g/L, NaC15g/L, agar 20g/L, pH value 7.0, and solvent is water; Bacillus subtilis ACCC01185 is transferred in fresh test tube slant from the inclined-plane of preserving, cultivate 24h, obtain slant strains for 37 ℃; (2) seed culture: liquid seed culture medium final concentration consists of: glucose 2g/L, NaC15g/L, yeast extract 5g/L, peptone l0g/L, pH7.0, solvent is water; By liquid seed culture medium sterilizing 30min under 120 ℃ of conditions, after cooling, from the cultured inclined-plane of step (1), with oese, choosing 1 ring is inoculated in the 300ml triangular flask that fills 50ml sterile liquid seed culture medium, putting 37 ℃ of temperature control shaking tables cultivates, rotating speed 220r/min, to gemma rate, reach and more than 90% (approximately need 24h) time stops, obtain bacillus subtilis ACCC01185 seed liquor, be bacillus subtilis ACCC01185 mycetocyte suspension.
Solid-state fermentation culture medium A of the present invention, solid-state fermentation culture medium B are solid-state fermentation culture medium, water a, and water b is water, and letter itself does not have implication.
Beneficial effect of the present invention is mainly reflected in: feather protein peptide feed addictive of the present invention can make feather protein digest and assimilate efficiency to reach 90%, contain Several Active Peptides, and ferment through multiple beneficial microorganism, through low temperature drying, form, be rich in keratinase, digestive ferment, active bacterium, have aid digestion and promote to grow; Be conducive to regulate and control animal intestinal beneficial microbe colony; Have natural fermenting aroma, good palatability, has good food calling effect, promotes young animal to search for food; Can obviously improve the allotriophagy of livestock and poultry, the animal protein feeds such as the complete Peru Fish Dietary of energy, SDPP, have obvious economic benefit and social benefit.
(4) specific embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in this:
The bent preparation of kind of embodiment 1 bacterial classification
(1) bacillus subtilis (Bacillus subtilis ACCC01185, Chinese agriculture microorganism fungus kind preservation administrative center provides):
Slant medium final concentration consists of: peptone 1g, beef extract 0.3g, NaC10.5g, agar 2.0g, distilled water 100ml, pH value 7.0.
Liquid seed culture medium is: glucose 0.2g, NaC10.5g, yeast extract 0.5g, peptone l g, distilled water 100mL, pH7.0.Sterilizing 30min under 120 ℃ of conditions.
Bacillus subtilis ACCC01185 is inoculated in to slant medium, cultivates 24h, obtain slant strains for 37 ℃; From slant strains picking one oese in filling the 300ml triangular flask of 50ml sterile liquid seed culture medium, putting 37 ℃ of temperature control shaking tables cultivates, rotating speed 220r/min, to gemma rate, reach and more than 90% (approximately need 24h) time stops, obtain bacillus subtilis ACCC01185 seed liquor, be bacillus subtilis ACCC01185 mycetocyte suspension.
(2) bacillus licheniformis (Bacillus licheniformis ACCC01198 is provided by Chinese agriculture microorganism fungus kind preservation administrative center):
Slant medium final concentration consists of: beef extract 5g, peptone 10g, sodium chloride 5g, agar 30g, distilled water 1000mL, pH value 7.4.
Liquid seed culture medium is: beef extract 5g, soy peptone 10g, NaC15g, distilled water 1L, pH7.0.
Bacillus licheniformis is transferred in fresh test tube slant from the inclined-plane of preserving, in 37 ℃ of cultivation 18h, obtain slant strains; Get cultured slant strains, with oese, choosing 1 ring is inoculated in the 250ml triangular flask that fills 50ml aseptic seed culture medium, then put 37 ℃ of vibrations (rotating speed is 120r/min) in swinging constant temperature speed governing shaking flask cabinet and cultivate 24h, obtain bacillus licheniformis ACCC01198 seed liquor, be bacillus licheniformis ACCC01198 mycetocyte suspension.
(3) saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC20042, Chinese agriculture microorganism fungus kind preservation administrative center provides):
The former bacterium test tube of saccharomyces cerevisiae---little triangular flask seed (maltose liquid glucose 100ml, 7～8 ° of Be of pol) 30 ℃ of heat insulating culture 10h---proceed to large triangular flask (maltose liquid glucose 600ml, 7～8 ° of Be of pol), 30 ℃ of heat insulating culture 10h---proceed to (syrup 13kg in Ka Shi tank, 7～8 ° of Be expansion cultivations of pol) 10h---Ka Shi tank yeast starter is spread cultivation at distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 20h, is saccharomyces cerevisiae seed liquor.
(4) saccharomycopsis fibuligera (Endomycopsisfibuligera ACCC20015, administrative center provides by the preservation of Chinese agriculture microorganism fungus kind):
The former bacterium test tube of saccharomycopsis fibuligera---little triangular flask seed (maltose liquid glucose 100ml, 7～8 ° of Be of pol) 30 ℃ of heat insulating culture 10h---proceed to large triangular flask (maltose liquid glucose 600ml, 7～8 ° of Be of pol), 30 ℃ of heat insulating culture 10h---proceed to (syrup 13kg in Ka Shi tank, 7～8 ° of Be expansion cultivations of pol) 10h---Ka Shi tank yeast starter is spread cultivation at distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg in 30 ℃ of cultivations, flour 30kg, wheat bran 10kg) total incubation time 20h, is saccharomycopsis fibuligera seed liquor.
Embodiment 2 feather protein peptide feed addictives
(1) preparation of yeast culture:
Described yeast culture preparation method: will be by wheat bran 600g, corn flour 30g, vinasse 150g, bean cake powder 200g, the fermentation substrate that urea 20g forms adds in sterilization tank, then adds 650g water, under 0.12Mpa pressure, boiling 35min carries out sterilizing, after sterilizing, close inlet valve, shelving 35min discharging, obtains solid-state fermentation culture medium.Ventilation blast-cold to 33～35 ℃, inoculate respectively saccharomyces cerevisiae ACCC20042 mycetocyte suspension 40g(viable count prepared by embodiment 1 method and reach 1.2 * 10 with solid-state fermentation culture medium dry weight basis
7cfu/g), (viable count reaches 2 * 10 with solid-state fermentation culture medium dry weight basis to the multiple film spore ferment ACCC20015 mycetocyte suspension 50g of button capsule
7cfu/g), fermentation temperature was controlled at 30 ℃ of fermented and cultured after 30 hours, is dried to moisture is less than 10% at 35 ℃, made yeast culture.
(2) preparation of feather protein peptide feed addictive
Feather powder (all can by various poultry feathers, wash clean, dry to moisture and be less than 10%, being crushed to fineness is 40 orders) after the yeast culture 80g for preparing of 920g and step (1) mixes, add 500g water, with cobalt-60, under 5kGy, carry out irradiation sterilization, obtain feather protein peptide solid-state fermentation culture medium.The bacillus licheniformis ACCC01198 mycetocyte suspension 1g(viable count of respectively being prepared by embodiment 1 method reaches 2 * 10 with solid-state fermentation culture medium dry weight basis
7cfu/g), bacillus subtilis ACCC01185 mycetocyte suspension 3g(viable count reaches 5 * 10 with solid-state fermentation culture medium dry weight basis
7cfu/g), blend fermented and cultured at 35-38 ℃.Fermentation temperature has mainly been managed in fermentation.Open temp auto-controlling apparatus (BYS-II mark is supported the wet auto-controlling apparatus of room temperature, Hebei province Hong Yu instrument and equipment Co., Ltd), connects fan power, and temperature upper control limit is 38 ℃, under be limited to 35 ℃.Diligent observation, diligent test.Temperature controller probe drop point wants correct, the degree of depth will reach 10cm(and plug in thermometer on can limit and contrast), mainly prevent probe, temperature controller and connection instrument fault, after general 6h, start to heat up, in early stage used chamber, wind is touring, when room temperature higher (conventionally reaching 36 ℃) or material temperature intensification too quickly, air door outside can opening chamber, reaches the object of growth regulation temperature.Complete through 30h fermentation, can strengthen air door, door and window, makes material temperature lower than 40 ℃.Adopt low temperature drying, early stage, material temperature was controlled in 38 ℃, and later stage material temperature is controlled in 45 ℃, and moisture is less than 10% can go out room.After oven dry, be crushed to fineness 40 orders, obtain moisture≤10%, crude protein quality content >=60% feather protein peptide feed addictive.
Embodiment 3 feather protein peptide feed addictives
(1) yeast culture preparation
Described yeast culture preparation method: will be by wheat bran 400g, corn flour 100g, vinasse 200g, bean cake powder 250g, the fermentation substrate that urea 50g forms adds in sterilization tank, adds 1000g water, under 0.12Mpa pressure, boiling 35min carries out sterilizing, after sterilizing, close inlet valve, shelving 35min discharging, obtains solid-state fermentation culture medium.Ventilation blast-cold to 33～35 ℃, inoculate respectively saccharomyces cerevisiae ACCC20042 mycetocyte suspension 120g(viable count prepared by embodiment 1 method and reach 3.6 * 10 with solid-state fermentation culture medium dry weight basis
7cfu/g), (viable count reaches 5 * 10 with solid-state fermentation culture medium dry weight basis to the multiple film spore ferment ACCC20015 mycetocyte suspension 125g of button capsule
7cfu/g) fermentation temperature is controlled at 30 ℃, and fermented and cultured, after 28 hours, is dried to moisture at 35 ℃～45 ℃ and is less than 10% altogether, makes yeast culture.
(2) preparation of feather protein peptide feed addictive
Feather meal (various poultry feathers all can, wash clean, dries to moisture and is less than 10%, being crushed to fineness is 20 orders) after the yeast culture 20g for preparing of 980g and step (1) mixes, add 1200g water, with cobalt-60, under 5kGy, carry out irradiation sterilization, obtain feather protein peptide solid-state fermentation culture medium.The bacillus licheniformis ACCC01198 mycetocyte suspension 2.5g(viable count of respectively being prepared by embodiment 1 method reaches 5 * 10 with solid-state fermentation culture medium dry weight basis
7cfu/g), bacillus subtilis ACCC01185 mycetocyte suspension 18g(viable count reaches 3 * 10 with solid-state fermentation culture medium dry weight basis
8cfu/g), blend fermented and cultured at 35～38 ℃.Other operations are with embodiment 2, complete through 45h fermentation, can strengthen air door, and door and window, does not make material temperature surpass 40 ℃, wait for out that room is dry.Adopt low temperature drying, early stage, material temperature was controlled in 38 ℃, and later stage material temperature is controlled in 45 ℃, and moisture is less than 10% can go out room.More than being crushed to fineness 60 orders after oven dry, obtain moisture≤10%,, crude protein >=60% feather protein peptide feed addictive.
Embodiment 4 feather protein peptide feed addictives
(1) preparation of yeast culture:
Described yeast culture preparation method: will be by wheat bran 500g, corn flour 65g, vinasse 175g, bean cake powder 225g, the fermentation substrate that urea 35g forms adds in sterilization tank, then adds 825g water, under 0.12Mpa pressure, boiling 35min carries out sterilizing, after sterilizing, close inlet valve, shelving 35min discharging, obtains solid-state fermentation culture medium.Ventilation blast-cold to 33～35 ℃, inoculate respectively saccharomyces cerevisiae ACCC20042 mycetocyte suspension 80g(viable count prepared by embodiment 1 method and reach 2.4 * 10 with solid-state fermentation culture medium dry weight basis
7cfu/g), (viable count reaches 3.5 * 10 with solid-state fermentation culture medium dry weight basis to the multiple film spore ferment ACCC20015 mycetocyte suspension 87.5g of button capsule
7cfu/g), fermentation temperature was controlled at 30 ℃ of fermented and cultured after 28 hours, is dried to moisture is less than 10% at 35 ℃, made yeast culture.
(2) preparation of feather protein peptide feed addictive
Feather meal (various poultry feathers all can, wash clean, dries to moisture and is less than 10%, being crushed to fineness is 35 orders) after the yeast culture 50g for preparing of 950g and step (1) mixes, add 850g water, with cobalt-60, at 5kGy, carry out irradiation sterilization, obtain feather protein peptide solid-state fermentation culture medium.The bacillus licheniformis ACCC01198 mycetocyte suspension 1.75g(viable count of respectively being prepared by embodiment 1 method reaches 3.5 * 10 with solid-state fermentation culture medium dry weight basis
7cfu/g), bacillus subtilis ACCC01185 mycetocyte suspension 10.5g(viable count reaches 1.75 * 10 with solid-state fermentation culture medium dry weight basis
8cfu/g), blend fermented and cultured at 35-38 ℃.Other operations are with embodiment 2, complete through 45h fermentation, can strengthen air door, and door and window, does not make material temperature surpass 40 ℃, wait for out that room is dry.Adopt low temperature drying, early stage, material temperature was controlled in 38 ℃, and later stage material temperature is controlled in 45 ℃, and moisture is less than 10% can go out room.More than being crushed to fineness 60 orders after oven dry, obtain moisture≤10%, crude protein quality content >=60% feather protein peptide feed addictive.
Embodiment 5
Choose 108 of the three way cross piglets of 28 ages in days wean, by nest not, sex, principle that body weight is close be divided into 3 groups (test I group, test II group and control groups), every group of 3 repetitions, each repeats l2 pig.Control group fed basal diet (quality formula is in Table 1), test I group, test II group are used respectively 2%(weight) and 5%(weight) the fish meal of the alternative basal diet moderate of feather protein peptide feed addictive prepared of embodiment 2 methods, experimental period 28-49 age in days, free choice feeding, production performance the results are shown in Table shown in 2.
Table 1 daily ration mass component forms
Compound premix is to contain in every kilogram of diet: iron 130mg, copper 200mg, zinc 130mg, manganese 40mg, iodine 0.35mg, selenium 0.30mg, retinol1 5750IU, neo dohyfral D3 500IU, vitamin E2 8IU, prokayvit .5mg, thiamine 3.5mg, riboflavin 8.75mg, nicotinic acid 35mg, pantothenic acid 17.5mg, Cobastab
64.2mg, folic acid 1.75mg, biotin 175g, choline 300mg.
Feather protein peptide feed addictive on the impact of Growth Performance of Weaning Piglets in Table 2.With 2% (test I group) and 5% (test II group) feather protein peptide feed addictive equivalent substitution fish meal, can obviously improve daily gain, reduce material anharmonic ratio.
Table 2 feather protein peptide feed addictive affects Growth Performance of Weaning Piglets
Note: the adjacent lowercase alphabet of colleague's data shoulder mark shows significant difference (P<O.05), and the capitalization person of being separated by represents difference extremely significantly (P<0.01).Following table is same
Embodiment 6
Choose healthy three way cross Du * 60, large * long 28 age in days weanling pig 6 nest, according to nest not, litter size, initial body heavy phase be unanimously divided into 2 groups at random: control group and test group, 3 every group repetitions (the piglet number of each repetition is 8-11 head).Control group is fed daily ration, and Diet Formula is in Table 3.Test group 8%(weight) the feather protein peptide feed addictive that prepared by embodiment 3 methods substitutes the 4%(weight in control group daily ration) fish meal and 4%(weight) dregs of beans, free choice feeding, 28-30 age in days is preliminary trial period, the 30-53 age in days formal test phase, formal test phase piglet growth situation is shown in Table 4.
Table 3 test daily ration quality forms
Premix is that every kilogram of diet contains: iron 130mg, copper 200mg, zinc 130mg, manganese 40mg, iodine 0.35mg, selenium 0.30mg, retinol1 5750IU, neo dohyfral D3 500IU, vitamin E2 8IU, prokayvit .5mg, thiamine 3.5mg, riboflavin 8.75mg, nicotinic acid 35mg, pantothenic acid 17.5mg, Cobastab
64.2mg, folic acid 1.75mg, biotin 175g, choline 300mg.
Table 4 shows, with 8% feather protein peptide feed addictive, substitutes 4% fish meal and 4% dregs of beans in control group daily ration, and Growth Performance of Weaning Piglets effect has clear improvement.
The impact of table 4 feather protein peptide feed addictive on Growth Performance of Weaning Piglets
Note: the adjacent lowercase alphabet of colleague's data shoulder mark shows significant difference (P<O.05), and the capitalization person of being separated by represents difference extremely significantly (P<0.01).
Embodiment 7
Select 1 age in days Ai Wei mattress broiler chicken 360 plumages, by feeding experiment requirement, be divided into 4 groups, be respectively control group, test group I, test group II, test group III; Each organizes 3 repetitions, and each repeats 30 plumage chickens.Control group is fed basal diet (basal diet formula is shown in Table 5), test I, test II group are used respectively 2%(weight) and 5%(weight) the feather protein peptide feed addictive prepared of embodiment 4 methods substitute the fish meal of basal diet moderate, test group III 8%(weight) the alternative basal diet of feather protein peptide feed addictive in 5%(weight) fish meal and 3%(weight) dregs of beans.Feeding process free choice feeding, feather protein peptide feed addictive the results are shown in Table shown in 6 the impact of growth of meat chicken performance.
The quality formula of table 5 test daily ration
In table 5, compound premix provides for every kilogram of daily ration: retinol1 2500IU, neo dohyfral D3 4125IU, vitamin e1 5IU, farnoquinone mg, thiamine 1mg, riboflavin 8.5mg, calcium pantothenate 50mg, nicotinic acid 32.5mg, pyridoxol 8mg, biotin 2mg, folic acid 5mg, Cobastab-5mg, choline 500mg, manganese 65mg, iodine 1mg, iron 60mg, copper 8mg, zinc 66nag, selenium 0.3mg.
Table 6 shows that the test group of applying feather protein peptide feed addictive can obviously improve broiler chicken 42 age in days body weight, average daily gain, reduction material anharmonic ratio.
The impact of table 6 feather protein peptide feed addictive on growth of meat chicken performance
Note: the adjacent lowercase alphabet of colleague's data shoulder mark shows significant difference (P<O.05), and the capitalization person of being separated by represents difference extremely significantly (P<0.01).
Claims (9)
1. a feather protein peptide feed addictive, it is characterized in that described feather protein peptide feed addictive prepared as follows: the preparation of (1) yeast culture: substrate is mixed with water a, obtain solid-state fermentation culture medium A, again saccharomyces cerevisiae and saccharomycopsis fibuligera are inoculated in solid-state fermentation culture medium A, at 30 ℃, cultivate 28～30h, after cultivation finishes by culture in 35～45 ℃ dry, obtain yeast culture; Described substrate is comprised of the raw material of following quality proportioning: wheat bran 40～60%, corn flour 3～10%, vinasse 15～20%, bean cake powder 20～25% and urea 2～5%; Described water a and substrate total mass ratio are 0.5～1:1; (2) preparation of feather protein peptide feed addictive: poultry feather is pulverized, obtain poultry feather powder, poultry feather powder is mixed with the described yeast culture of step (1), add water b, stir, obtain solid-state fermentation culture medium B, respectively bacillus licheniformis and bacillus subtilis are seeded in solid-state fermentation culture medium B to 35～38 ℃ of fermented and cultured 30～45h, after fermented and cultured finishes, tunning be dry, pulverize, obtain described feather protein peptide feed addictive; The mass ratio that feeds intake of described poultry feather powder and yeast culture is 9～49:1, and the addition of described water b and yeast culture and poultry feather powder gross mass ratio are 0.5～1.2:1.
2. feather protein peptide feed addictive as claimed in claim 1, is characterized in that the described saccharomyces cerevisiae of step (1) and saccharomycopsis fibuligera add with the form of mycetocyte suspension respectively.
3. feather protein peptide feed addictive as claimed in claim 2, is characterized in that the addition of the described saccharomyces cerevisiae mycetocyte of step (1) suspension counts 1.2～3.6 * 10 with viable count
7cfu/g solid-state fermentation culture medium A dry weight.
4. feather protein peptide feed addictive as claimed in claim 2, is characterized in that the addition of the described saccharomycopsis fibuligera mycetocyte of step (1) suspension counts 2～5 * 10 with viable count
7cfu/g solid-state fermentation culture medium A dry weight.
5. feather protein peptide feed addictive as claimed in claim 1, is characterized in that the described poultry feather powder of step (2) moisture is less than 10%, and fineness is 20～40 orders.
6. feather protein peptide feed addictive as claimed in claim 1, is characterized in that the described bacillus licheniformis of step (2) and bacillus subtilis add with the form of mycetocyte suspension respectively.
7. feather protein peptide feed addictive as claimed in claim 6, is characterized in that the addition of described bacillus licheniformis mycetocyte suspension counts 2～5 * 10 with viable count
7cfu/g solid-state fermentation culture medium B dry weight.
8. feather protein peptide feed addictive as claimed in claim 6, is characterized in that the addition of described bacillus subtilis mycetocyte suspension counts 5～30 * 10 with viable count
7cfu/g solid-state fermentation culture medium B dry weight.
9. feather protein peptide feed addictive as claimed in claim 1, it is characterized in that described feather protein peptide feed prepared as follows: the preparation of (1) yeast culture: substrate is mixed with water a, under 0.12MPa pressure, boiling 35min carries out sterilizing, shelving 35min again, obtain solid-state fermentation culture medium A, again saccharomyces cerevisiae ACCC20042 mycetocyte suspension and saccharomycopsis fibuligera ACCC20015 mycetocyte suspension are inoculated into respectively in solid-state fermentation culture medium A, at 30 ℃, cultivate 28～30h, after cultivation finishes, culture is dry at 35～45 ℃, obtain yeast culture, described substrate is comprised of the raw material of following quality proportioning: wheat bran 40～60%, corn flour 3～10%, vinasse 15～20%, bean cake powder 20～25% and urea 2～5%, the ratio of described water a and substrate gross mass is 0.65～1:1, the addition of described saccharomyces cerevisiae ACCC20042 mycetocyte suspension counts 1.2 * 10 with viable count
7cfu/g solid-state fermentation culture medium A dry weight, the addition of described saccharomycopsis fibuligera ACCC20015 mycetocyte suspension counts 2 * 10 with viable count
7cfu/g solid-state fermentation culture medium A dry weight, (2) preparation of feather protein peptide feed addictive: poultry feather powder is mixed with mass ratio 11.5～49:1 with the described yeast culture of step (1), add water b, stir, with cobalt-60 radiation sterilization under 5kGy, obtain solid-state fermentation culture medium B, respectively by bacillus licheniformis ACCC01198 mycetocyte suspension and bacillus subtilis ACCC01185 mycetocyte suspension inoculation to solid-state fermentation culture medium B, 35～38 ℃ of fermented and cultured 30～45h, after fermented and cultured finishes, tunning is dried to moisture at 45 ℃ and is less than 10%, being crushed to fineness is 40～60 orders, obtain described feather protein peptide feed addictive, described poultry feather powder moisture is less than 10%, and fineness is 20～40 orders, the ratio of described water b and yeast culture and poultry feather powder gross mass is 0.5～1.2:1, the addition of described bacillus licheniformis mycetocyte suspension counts 2～5 * 10 with viable count
7cfu/g solid-state fermentation culture medium B dry weight, the addition of described bacillus subtilis mycetocyte suspension counts 5～8 * 10 with viable count
7cfu/g solid-state fermentation culture medium B dry weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210201404.8A CN102726596B (en) | 2012-06-18 | 2012-06-18 | Feather protein peptide feed additive and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210201404.8A CN102726596B (en) | 2012-06-18 | 2012-06-18 | Feather protein peptide feed additive and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102726596A CN102726596A (en) | 2012-10-17 |
| CN102726596B true CN102726596B (en) | 2014-03-26 |
Family
ID=46983472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210201404.8A Expired - Fee Related CN102726596B (en) | 2012-06-18 | 2012-06-18 | Feather protein peptide feed additive and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102726596B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053833A (en) * | 2012-12-20 | 2013-04-24 | 福建省农业科学院中心实验室 | Philippines eel feed containing feather peptide powder |
| CN103947850B (en) * | 2014-03-13 | 2015-07-29 | 杭州启明星生物科技研究院有限公司 | A kind of fowl allotriophagy conditioning agent and application |
| CN105454658A (en) * | 2015-12-21 | 2016-04-06 | 合肥工业大学 | Preparation method of feather fermentation protein feed |
| CN106071104A (en) * | 2016-06-14 | 2016-11-09 | 杭州百瑞特饲料科技有限公司 | A kind of expanded fermentation protein feedstuff additive and application |
| CN106689692A (en) * | 2016-11-25 | 2017-05-24 | 彭程 | Protein feed for substituting soybean meal or fish meal |
| CN107594090A (en) * | 2017-10-30 | 2018-01-19 | 广州昆虫蛋白生物科技有限公司 | A kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff |
| CN109287855A (en) * | 2018-09-28 | 2019-02-01 | 青岛农业大学 | A kind of fermentation process of corn stover, the fermented maize stalk of this method preparation and application |
| CN112655825A (en) * | 2020-12-23 | 2021-04-16 | 安徽希普生物科技有限公司 | Feather protein peptide particle composition and preparation method thereof |
| CN115067435A (en) * | 2022-06-24 | 2022-09-20 | 广东省科学院生物与医学工程研究所 | High-protein pigeon feed taking waste feathers, pericarps and schizochytrium limacinum fungus residues as raw materials and preparation method thereof |
| CN115606678A (en) * | 2022-10-08 | 2023-01-17 | 广东省科学院生物与医学工程研究所 | Method for preparing high-protein probiotic animal feed by using waste feathers through composite feeding probiotics |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067922A (en) * | 1992-06-19 | 1993-01-13 | 朱妙云 | Nitrogen source for fermentation in production of jinggangmycin |
| CN101285086B (en) * | 2007-12-29 | 2012-05-23 | 青岛蔚蓝生物集团有限公司 | Feather products, processing technology and use thereof |
| CN101240302B (en) * | 2008-03-14 | 2010-11-10 | 山东省农业科学院高新技术研究中心 | Method for producing L-phenylalanine by using bacillus subtilis to ferment feather |
| CN101720853A (en) * | 2009-10-19 | 2010-06-09 | 李军训 | Technology for fermenting and decomposing protein feed with protease in two steps by using probiotics |
| CN102326668B (en) * | 2011-08-24 | 2012-11-28 | 农业部沼气科学研究所 | Method for anaerobically degrading feather keratin with microbial strain 18D-TA |
-
2012
- 2012-06-18 CN CN201210201404.8A patent/CN102726596B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102726596A (en) | 2012-10-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102726596B (en) | Feather protein peptide feed additive and application thereof | |
| CN102168045B (en) | Bacillus subtilis preparation and preparation method thereof | |
| CN101449739B (en) | Micro-ecology fermentation protein feedstuff and preparation method thereof | |
| CN104293697B (en) | A kind of chicken feed probiotics containing enterococcus faecalis and preparation method thereof | |
| CN106591182B (en) | A kind of probiotics of fermentation of bacillus subtilis Radix Astragali and its application | |
| CN102505003B (en) | High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof | |
| CN102907563B (en) | Method for preparing high-activity probiotic preparation for livestock breeding | |
| CN103444985B (en) | Feed additive and manufacturing method and application thereof | |
| CN104054902A (en) | Process for producing fermented soybean meal through solid state fermentation of mixed culture | |
| CN107494911B (en) | Method for breeding animals by using antibiotic-free feed containing astragalus stem, leaf and flower | |
| CN106974063B (en) | Method for producing high-efficiency protein feed by using feed enzyme in cooperation with bacillus coagulans | |
| CN103966124A (en) | Compound microecological preparation and additive, as well as premix thereof | |
| CN106260540A (en) | A kind of biological feedstuff for creep feed and creep feed | |
| CN104212735A (en) | Poultry composite probiotic preparation and preparation method thereof | |
| CN106721261A (en) | One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof | |
| CN106260504A (en) | A kind of method utilizing beer yeast slurry to produce fermentable moist forage | |
| CN103340277A (en) | Feeding microecological additive and compound feed containing same | |
| CN104247878A (en) | Compound pig feed capable of improving body immunity | |
| CN106721026A (en) | A kind of nonreactive laying cycle of laying hens compound premixed feed and preparation method and application | |
| CN112136965B (en) | Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof | |
| CN106071104A (en) | A kind of expanded fermentation protein feedstuff additive and application | |
| CN103205375B (en) | Quality clostridium butyricum for breeding egg-laying poultry | |
| CN101836688B (en) | Preparation method of antibiotic-free microbial fermentation feed | |
| CN108432979A (en) | A kind of Australia freshwater lobster ecological feed | |
| CN102406068A (en) | Method for preparing microbial fermented feed capable of keeping intestinal health of pigs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140326 Termination date: 20160618 |