CN102517209A - Multichannel cell stretch stress loading control meter - Google Patents
Multichannel cell stretch stress loading control meter Download PDFInfo
- Publication number
- CN102517209A CN102517209A CN2011103772698A CN201110377269A CN102517209A CN 102517209 A CN102517209 A CN 102517209A CN 2011103772698 A CN2011103772698 A CN 2011103772698A CN 201110377269 A CN201110377269 A CN 201110377269A CN 102517209 A CN102517209 A CN 102517209A
- Authority
- CN
- China
- Prior art keywords
- cell culture
- unit
- vacuum chamber
- cell
- vakuumkammer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Electromagnetism (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a multichannel cell stretch stress loading control meter, which comprises a cell culture unit, a power loading unit and a control detection unit, wherein the cell culture unit comprises a vacuum chamber and an elastic film culture plate which is placed on the vacuum chamber and sealed with the vacuum chamber. An elastic film serves as a cavity wall of the vacuum chamber. The power loading unit is a vacuum pump and is used for vacuumizing the vacuum chamber according to setting. The control detection unit is used for controlling opening and closing of a solenoid valve arranged on a cell culture unit gas circuit channel, a venting three-way solenoid valve and the vacuum pump according to setting, controls pressure of the vacuum chamber of the cell culture unit to obtain the required deformation degree, and achieves the goal of controlling deformation degree of the elastic film of the cell culture unit.
Description
Technical field
The present invention relates to a kind of hyperchannel cell stretching stress Loading Control appearance, belong to the laboratory apparatus technical field.
Background technology
Growing of cell and tissue is the cytobiology phenomenon on the molecular level, and stress is the important external informations that influence cellularstructure and function as a kind of stimulating factor of cellular elements regulation and control.In vivo, biological behaviour such as the growth of cell, propagation, secretion and their residing mechanical environments are undivided.Cell can normally be brought into play its function in normal physiological mechanics environment, still, after the cell peripheral mechanical environment changed, the various functions of cell also changed thereupon.A lot of scholar's application cell Experiments of Machanics methods; The histocyte of vitro culture is applied the stress-strain stimulation of simulated in vivo environment through mechanism; Combining form is learned, the molecular biology measuring technology, has carried out the research of aspects such as the growth under various ambient stresses of a large amount of cells, metabolism and intracellular protein, cytokine-expressing variation.The research of cyto-mechanics experiment at present is that modern biomechanics developed field, forward position very rapidly in recent years, is a research focus that is rising both at home and abroad.It relates to the research of aspects such as variation of distortion, adhesive power of metabolism, cytolemma and cytoskeleton of secretion, the extracellular matrix of the growing multiplication of cell under the load effect, cytokine.
The basis of cyto-mechanics research and key are the cell loading techniques.The size of human body cell is unit with the micron, and macroscopical experimental technique and mechanics loading method can't be applied directly to the research of cell levels.So relevant biomeasurement means are matters of utmost importance that cyto-mechanics faces after setting up suitable loading culture model and seeking suitable cytomorphosis.As far back as nineteen thirty-nine, Glucksmann has carried out ground-breaking research on the mechanics loading method of cultured cell in vitro.He cultivates the shin bone endo cell of Embryo Gallus domesticus on paired intercostal muscle matrix, when amyotrophy traction rib each other near the time, the cell of isolated culture has promptly received the effect of pressure.We can say that this is a cyto-mechanics stress model the earliest.Process is constantly improved and development, and people have proposed the Experiments of Machanics method of multiple isolated culture cell, develops dissimilar and experimental installation function, on the basis of qualitative analysis, develops into the mechanical characteristic of ability quantitative examination cell.
Carry out the stress of cells in-vitro is loaded through cell in vitro culture technique, application cell mechanical loading unit, become the important research means of cyto-mechanics.
The ambient stress that each cell is survived, electric physiological environment and body fluid environment are far from each other, and the reaction of each cell counter stress also can not be just the same; So stress is in the cell of vitro culture, different afterburning modes, the possibility of result that different cells obtains is also different; Even with a kind of cell, such as periodontal ligament cell, when this cell is returned to internal milieu; The effect that is drawn also can not be just the same, do not conform to different tension types, effect amplitude in the conclusion that experiment in vitro drew that also might be simple and the body; The influence that pair cell caused also possibly be different, possibly be antipodal sometimes.
Summary of the invention
Technical problem to be solved by this invention is that the deficiency that is directed against prior art provides a kind of hyperchannel cell stretching stress Loading Control appearance.
Hyperchannel cell stretching stress Loading Control appearance comprises cell culture unit, power loading unit, control detecting unit; Said cell culture unit comprises Vakuumkammer and the elastica culture plate that is placed on the Vakuumkammer and is sealed with Vakuumkammer, and elastica is as a chamber wall of Vakuumkammer; Said power loading unit is a vacuum pump, is used for according to setting Vakuumkammer being vacuumized; The control detecting unit is used for according to the opening and closing of setting SV, venting three-way solenoid valve and vacuum pump on the control cell culture unit gas channels; The deformation ratio of controlling the cell culture unit vacuum chamber pressure and needing obtaining reaches the purpose of controlling cell culture unit elastica deformation quantity.
Described hyperchannel cell stretching stress Loading Control appearance; Said control detecting unit comprises pressure transmitter, signal amplification and modulate circuit, A/D change-over circuit and micro-chip; Pressure transmitter converts the pressure change in the cell culture unit Vakuumkammer to voltage signal; Amplify, after the filtering process, convert the manageable vacuum chamber pressure sampled signal of micro-chip into through signal conditioning circuit by the A/D change-over circuit; Micro-chip compares the current vacuum chamber pressure sampled signal and the vacuum chamber pressure of default, according to the opening and closing of SV, venting three-way solenoid valve and vacuum pump on the comparative result control gas channels.
Hyperchannel cell stretching stress load apparatus of the present invention can carry out the contrast experiment of triple channel different deformation rate simultaneously; And volume is little, is easy to carry, and deformation ratio, frequency can accurately be regulated, and be simple to operate, and the deformation ratio of 1%～21% scope can be provided, and the strain frequency is adjustable in 0～0.5Hz scope.System is stable, has reached design requirements fully, and the experiment in vitro method that the histocyte of adherent growth is applied basilar membrane stretching stress is provided.
Description of drawings
Fig. 1 is control detecting unit structure principle chart;
Fig. 2 is that T-valve, SV connect synoptic diagram.
Embodiment
Below in conjunction with specific embodiment, the present invention is elaborated.
Cell stretching stress Loading Control appearance comprises cell culture unit, power loading unit, control detecting unit;
Wherein, elastica 6 well culture plates that said cell culture unit comprises Vakuumkammer (synthetic glass material) and is placed on the Vakuumkammer and is sealed with Vakuumkammer, elastica is as a chamber wall of Vakuumkammer.
Wherein, said power loading unit is a vacuum pump, is used for according to setting the Vakuumkammer in the cell culture unit being vacuumized; When the vacuum pump work; During the indoor air of extracting vacuum; Because Vakuumkammer forms negative pressure, the elastica that is positioned at the culture plate of Vakuumkammer top will cave in to internal vacuum chamber, tensile deformation downwards; The cell that is inoculated in the elastica culture plate receives stretching action with the distortion of elastica, produces deformation.
Control detecting unit structured flowchart is as shown in Figure 1; The control detecting unit is used for according to the opening and closing of setting SV, venting three-way solenoid valve and vacuum pump on control cell culture unit (three) gas channels; The deformation ratio of controlling the cell culture unit vacuum chamber pressure and needing obtaining reaches the purpose of controlling cell culture unit elastica deformation quantity.
As shown in Figure 1; Pressure transmitter converts the pressure change in the cell culture unit Vakuumkammer to voltage signal; After the processing of links such as signal conditioning circuit amplification, filtering, convert the manageable vacuum chamber pressure sampled signal of micro-chip into by the A/D change-over circuit; (set by the manual work input, and can when system works, adjust at any time by this value according to current vacuum chamber pressure sampled signal and the vacuum chamber pressure of default for micro-chip.Set(ting)value is stored in the non-volatile memory, directly calls during system boot, avoids each the use all to want the artificial experiment parameter of setting.) compare, according to the opening and closing of SV, venting three-way solenoid valve and vacuum pump on the comparative result control gas channels.
Between 0～25kPa, selecting M1451 pressure transmitter standard range ability is the model of 0～5psi (1psi ≈ 6.897kPa) according to cell culture unit vacuum chamber pressure variation range.
Amplify and signal conditioning circuit: the output analog voltage signal of M1451 pressure transmitter is handled through links such as amplification, filtering, just can reach the input requirement of A/D change-over circuit.
A/D change-over circuit: MAX1292 is 12 analog to digital converters, and the analog signal conversion that is used for transmitter is produced is the manageable numerary signal of computingmachine.
Relation between cell culture unit elastica deformation ratio and the cell culture unit vacuum chamber pressure is:
y＝0.0306x
2+0.4307x+0.0175， (1)
Y is a deformation ratio in the formula, the % of unit; X is the gauge pressure force value, the kPa of unit.After system powered on and sets experiment parameter, micro-chip converted deformation ratio to corresponding force value according to this formula, and is placed in the interior data storer.After the experiment beginning, micro-chip at any time should value and detected cell culture unit vacuum chamber pressure value compare, and control corresponding solenoid valve according to comparative result and move.Thereby reach the purpose that obtains the deformation ratio of needs through control cell culture unit vacuum chamber pressure.Described vacuum chamber pressure is with gauge pressure form reflection, and the reflection of the output voltage of M1451 pressure transmitter is detected absolute pressure, therefore must convert this absolute pressure into gauge pressure.In pressure survey, the branch of absolute pressure, gauge pressure is often arranged, so-called absolute pressure is meant that measured medium acts on the whole pressure on the container unit area, representes with Pj.The mean pressure that ground air column produced is called barometric point, representes with Pq.Absolute pressure and barometric point poor is called gauge pressure, is called for short gauge pressure, representes to be Pb=Pj-Pq with Pb.According to the conversion formula Pb=Pj-Pq between gauge pressure and the absolute pressure, can convert the absolute pressure that the M1451 pressure transmitter detects into gauge pressure pressure that system need handle.Pb is a gauge pressure pressure in the formula, and Pj is the absolute pressure value that pressure transmitter detects, and Pq is current atmospheric value, and this is an absolute pressure.
With reference to figure 2, the logic that pair cell is cultivated unit vacuum chamber pressure control is: (one) four SV powers up.SV 1-3 all connects, and three-way solenoid valve AC turn-offs, and AB connects, thereby three cell culture unit Vakuumkammers are all connected with pressure transmitter and vacuum pump.(2) start vacuum pump.The action of bleeding of vacuum pump can make the negative pressure in the cell culture unit Vakuumkammer that ascending variation takes place.(3) when ducted negative pressure reaches minimum one of three cell culture unit vacuum indoor pressure set(ting)values, the SV that links to each other with this cell culture unit will cut out; Along with ducted negative pressure value increases, when reaching big and peak pressure set(ting)value successively, corresponding solenoid valve also can be closed successively.The cell culture unit Vakuumkammer will remain on negative pressure state.(4) all reach set(ting)value separately when three cell culture unit vacuum indoor pressures, after three SV 1-3 all closed, vacuum pump quit work, and three-way solenoid valve discharges.(5) cell culture unit is pressed default time maintenance negative pressure state, opens SV 1-3 then, makes the cell culture unit vacuum indoor pressure return to the ambient atmosphere pressure pressure state, and fexible film recovers ortho states.(6) behind the maintenance setting-up time, recirculation () is to the step of (five) once more.
Need not set up the data sheet between deformation ratio and the pressure in the native system design; But before system works; The parameter of the cell culture unit deformation ratio that each passage of setting according to manual work is corresponding; Relation (formula (1)) through pressure transmitter output signal and fexible film deformation ratio converts the deformation ratio parameter into gauge pressure pairing numerical value, and the result's (real-time detected negative pressure value) with the AD sampling compares then, and controls the working order of corresponding solenoid valve and vacuum pump according to the result.
Should be understood that, concerning those of ordinary skills, can improve or conversion, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention according to above-mentioned explanation.
Claims (2)
1. a hyperchannel cell stretching stress Loading Control appearance is characterized in that, comprises cell culture unit, power loading unit, control detecting unit; Said cell culture unit comprises Vakuumkammer and the elastica culture plate that is placed on the Vakuumkammer and is sealed with Vakuumkammer, and elastica is as a chamber wall of Vakuumkammer; Said power loading unit is a vacuum pump, is used for according to setting Vakuumkammer being vacuumized; The control detecting unit is used for according to the opening and closing of setting SV, venting three-way solenoid valve and vacuum pump on the control cell culture unit gas channels; The deformation ratio of controlling the cell culture unit vacuum chamber pressure and needing obtaining reaches the purpose of controlling cell culture unit elastica deformation quantity.
2. hyperchannel cell stretching stress Loading Control appearance according to claim 1; It is characterized in that; Said control detecting unit comprises pressure transmitter, signal amplification and modulate circuit, A/D change-over circuit and micro-chip; Pressure transmitter converts the pressure change in the cell culture unit Vakuumkammer to voltage signal, amplifies, after the filtering process, converts the manageable vacuum chamber pressure sampled signal of micro-chip into by the A/D change-over circuit through signal conditioning circuit; Micro-chip compares the current vacuum chamber pressure sampled signal and the vacuum chamber pressure of default, according to the opening and closing of SV, venting three-way solenoid valve and vacuum pump on the comparative result control gas channels.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103772698A CN102517209A (en) | 2011-11-24 | 2011-11-24 | Multichannel cell stretch stress loading control meter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103772698A CN102517209A (en) | 2011-11-24 | 2011-11-24 | Multichannel cell stretch stress loading control meter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102517209A true CN102517209A (en) | 2012-06-27 |
Family
ID=46288280
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103772698A Pending CN102517209A (en) | 2011-11-24 | 2011-11-24 | Multichannel cell stretch stress loading control meter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102517209A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719355A (en) * | 2012-07-19 | 2012-10-10 | 苏州大学 | Cell vacuum absorption fixing device |
| WO2020020822A1 (en) * | 2018-07-25 | 2020-01-30 | Cytena Gmbh | Device having at least one container |
| CN113801791A (en) * | 2021-03-26 | 2021-12-17 | 中国海洋大学 | Multichannel uniform tensile stress in-vitro cell culture device and working method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
-
2011
- 2011-11-24 CN CN2011103772698A patent/CN102517209A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
Non-Patent Citations (1)
| Title |
|---|
| 张晓东: "牵张应力对牙周膜成纤维细胞及细胞外基质代谢影响的研究", 《中国博士学位论文全文数据库》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719355A (en) * | 2012-07-19 | 2012-10-10 | 苏州大学 | Cell vacuum absorption fixing device |
| WO2020020822A1 (en) * | 2018-07-25 | 2020-01-30 | Cytena Gmbh | Device having at least one container |
| CN113801791A (en) * | 2021-03-26 | 2021-12-17 | 中国海洋大学 | Multichannel uniform tensile stress in-vitro cell culture device and working method |
| CN113801791B (en) * | 2021-03-26 | 2022-08-02 | 中国海洋大学 | Multichannel uniform tensile stress in-vitro cell culture device and working method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201737949U (en) | Cell culturing device capable of periodically converting positive pressure and negative pressure | |
| CN102517209A (en) | Multichannel cell stretch stress loading control meter | |
| CN2818484Y (en) | Cell tractive tension controller | |
| DE602005019883D1 (en) | BIOREACTOR FOR EXAMINING THE EFFECTS OF FORCED STIMULI ON CELL ACTIVITY | |
| CN212025366U (en) | Cell tissue culture device capable of accurately controlling gas pressure and concentration | |
| CN101717719A (en) | In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof | |
| WO2003008535A3 (en) | Methods of generating human cardiac cells and tissues and uses thereof | |
| EP2336293A3 (en) | Cell culture methods and devices utilizing gas permeable materials | |
| ATE371743T1 (en) | METHOD FOR PRODUCING A TARGET SUBSTANCE BY FERMENTATION USING A BACTERIAL STRAIN LACKING THE ARCA GENE | |
| WO2011014674A3 (en) | Microfluidic device for pharmacokinetic-pharmacodynamic study of drugs and uses thereof | |
| DK1483373T3 (en) | Surgical device for skin therapy or testing | |
| EP1428869A4 (en) | Cell and structure incubator | |
| CN211645272U (en) | Multichannel cell stretching stress loading device | |
| CN103146574B (en) | A kind of high-throughput micro-fluidic biological mechanics long-time stimulus system and application thereof | |
| CN102967695B (en) | Soil body water saturation-drainage circulation compression test method and device | |
| CN101906379A (en) | Device for precisely stretching visual cells under simulated in vivo environment | |
| CN201819768U (en) | Full-automatic vacuum calibrator | |
| CN201408122Y (en) | Airtight cabin pressure regulator product testing device | |
| CN201737929U (en) | Precision visualization cell stretching device under environment simulating inner environment of human body | |
| CN205246522U (en) | Blood sugar tests data acquisition facility | |
| CN113801791B (en) | Multichannel uniform tensile stress in-vitro cell culture device and working method | |
| CN104357542A (en) | Method for determining activity of trichophyton rubrum keratinase | |
| Hatton et al. | Kinetics of stem gravitropism in Coprinus cinereus: determination of presentation time and ‘dosage-response’relationships using clinostats | |
| EP1645625B8 (en) | Polymer Substrat for Cell Cultivation, Especially Keratinocyte Cultivation | |
| CN221094175U (en) | Cell compression test device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120627 |