CN102641520A - Preparation method of biological repair nano dressing containing wood frog skin polypeptide and being used for wounds and burn - Google Patents
Preparation method of biological repair nano dressing containing wood frog skin polypeptide and being used for wounds and burn Download PDFInfo
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- CN102641520A CN102641520A CN2012101370331A CN201210137033A CN102641520A CN 102641520 A CN102641520 A CN 102641520A CN 2012101370331 A CN2012101370331 A CN 2012101370331A CN 201210137033 A CN201210137033 A CN 201210137033A CN 102641520 A CN102641520 A CN 102641520A
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Abstract
The invention aims at providing a preparation method of a biological repair nano dressing containing wood frog skin polypeptide and being used for wounds and burn, which is simple. The prepared biological repair nano dressing has good adhesion and bacteriostasis functions, and is safe, non-toxic and convenient to use. The nano dressing containing the wood frog skin polypeptide is used for treating the wounds, can achieve effects of wound surface insulation, hemostasis, pain stopping, moisture preservation, ventilation and anti-infection, replaces traditional dressing, can cover the wound surface, simultaneously participates in and promotes healing of the wound surface actively, and is a novel biological dressing.
Description
Technical field
The invention relates to and add the method for preparing that wood frog skin mixed polypeptide is used for the biology repairing nanometer dressing of wound burn.
Technical background
Along with going deep into of researching wound healing; People recognize that gradually the purpose of using dressing is far from for flap coverage; Dressing also must can help wound healing; It is to create an environment that promotes wound healing that wound is used the main purpose of applying, and temporarily plays the skin barrier function, for wound healing is created a favourable microenvironment.Traditional biological dressing comprises alloskin, Corii Sus domestica and artificial skin etc.Cadaver skin is considered to the most desirable raw material in the alloskin, but the cadaver skin source is difficult, costs an arm and a leg, and the danger that rejection is arranged and spread disease (comprising hepatitis and AIDS); The anti-sense power of Corii Sus domestica is relatively poor, influences epithelial growth; Artificial skin does not still have ideal especially product both at home and abroad.
Summary of the invention
The present invention aims to provide the method for preparing that the biology that is used for the wound burn is repaired nanometer dressing, and this method for preparing is simple, and the prepared biological dressing of repairing has good adhesiveness, bacteria resistance function, and safety, nontoxic, convenient in application.
The biology of indication is repaired in the dressing and has been added wood frog skin mixed polypeptide among the present invention, and its effect is to utilize the synergism of somatomedin to come the synthetic and division of inducing cell DNA, and its structure and human-body biological skin are roughly the same.The nanometer dressing of in the treatment wound, use adding wood frog skin mixed polypeptide can reach isolated wound surface hemostasis, pain relieving are arranged again, preserve moisture, breathe freely, the anti-infective effect.Thereby the replacement traditional dressing, its on the one hand can flap coverage, and simultaneously, initiatively participate in and promote wound healing, be a kind of new bio dressing.
Electrostatic spinning technique is used in the biological preparation of repairing nanometer dressing of indication among the present invention.Zein is dissolved in the solvent, adds an amount of wood frog skin mixed polypeptide, blend obtains the spinning mixed solution.Regulate the process conditions of spinning: voltage, distance, extruded velocity etc., collect the biology repairing nanometer dressing that obtains adding the Rana sylvatica Le conte skin active component.Its concrete preparation process is following:
(1) configuration spin solvent.
(2) Biodegradable material is dissolved in the solvent, obtains electricity and spin solution, add wood frog skin mixed polypeptide then in solution, magnetic agitation obtains the spinning mixed solution.
(3) gathering-device is installed,, is had part and residue on the aluminium foil owing to be difficult for being kept perfectly in the process of fibrous membrane under removing with the aluminium foil reception merely.So improve gathering-device, make its complete nanofiber mats that more is easy to get.
(4) blend spinning solution is poured in the plastics needle tubing.Outer and the gathering-device of plastic tube connects the positive and negative electrode of DC high-voltage power supply respectively, and certain extruded velocity is set, and the distance of the voltage of spinning and spinning nozzle to receptor is carried out spinning.
(5) sample places vacuum drying oven after dash receiver takes off.
Description of drawings
Fig. 1. the biological nanometer dressing pictorial diagram of repairing.
Fig. 2. the biological nanometer dressing stereoscan photograph of repairing.
The specific embodiment
Below be embodiments of the invention, these embodiment only are used for illustrative purposes, do not constitute limitation of the scope of the invention.
Embodiment 1
1.5-4.0 is restrained in the mixed solvent that zein is dissolved in the second alcohol and water; The volume ratio of second alcohol and water is 80: 20,70: 30, is made into the solution of 10%-40% (w/v) at 60: 40; It is an amount of to add wood frog skin mixed polypeptide then, and blend obtains the spinning solution of homogeneous.Spinning solution is added in the plastic injector, and syringe needle connects the high pressure positive electric field, the dash receiver upper berth one deck aluminium foil; Distance is controlled at 5cm～25cm between injector head and reception thing, and solution flow rate 0.5mL/h～2mL/h, voltage are 10kV～20kV; Carry out electricity and spin, collect and obtain nanofiber mats.
Embodiment 2
1.5-4.0 is restrained in the mixed solvent that zein is dissolved in the second alcohol and water; The volume ratio of second alcohol and water is 80: 20,70: 30, is made into the solution of 10%-40% (w/v) at 60: 40; It is an amount of to add wood frog skin mixed polypeptide then, and blend obtains the spinning solution of homogeneous.Spinning solution is added in the plastic injector, and syringe needle connects the high pressure positive electric field, the dash receiver upper berth one deck aluminium foil; Adhere to one deck adsorptive pads on the aluminium foil again; Distance is controlled at 5cm～25cm between injector head and reception thing, and solution flow rate 0.5mL/h～2mL/h, voltage are 10kV～20kV; Carry out electricity and spin, collect the adsorptive pads that obtains the attached nanofibers layer.
Embodiment 3
1.5-4.0 is restrained in the mixed solvent that zein is dissolved in the second alcohol and water; The volume ratio of second alcohol and water is 80: 20,70: 30, is made into the solution of 10%-40% (w/v) at 60: 40; It is an amount of to add wood frog skin mixed polypeptide then, and blend obtains the spinning solution of homogeneous.Spinning solution is added in the plastic injector, and syringe needle connects the high pressure positive electric field, the dash receiver upper berth one deck aluminium foil; Adhere to one deck medical adhesive-bonded fabric on the aluminium foil again; Distance is controlled at 5cm～25cm between injector head and reception thing, and solution flow rate 0.5mL/h～2mL/h, voltage are 10kV～20kV; Carry out electricity and spin, collect the medical adhesive-bonded fabric that obtains the attached nanofibers layer.
Claims (6)
1. interpolation wood frog skin mixed polypeptide is used for the method for preparing of the biology repairing nanometer dressing of wound burn, and its preparation method is characterised in that following steps:
(1) configuration spin solvent.
(2) Biodegradable material is dissolved in the solvent, obtains electricity and spin solution, add wood frog skin mixed polypeptide then
In solution, magnetic agitation obtains the spinning mixed solution.
(3) gathering-device is installed,, is had part and residue on the aluminium foil owing to be difficult for being kept perfectly in the process of fibrous membrane under removing with the aluminium foil reception merely.So improve gathering-device, make its complete nanofiber mats that more is easy to get.
(4) blend spinning solution is poured in the plastics needle tubing.Outer and the gathering-device of plastic tube connects the positive and negative electrode of DC high-voltage power supply respectively, and certain extruded velocity is set, and the distance of the voltage of spinning and spinning nozzle to receptor is carried out spinning.
(5) sample places vacuum drying oven after dash receiver takes off.
2. according to the method for preparing of claim 1, it is characterized in that the spin solvent that step 1) disposes must be harmless to human non-toxic.
3. according to the method for preparing of claim 1, it is characterized in that step 2) concentration of dope that disposed is 10%-40% (w/v).
4. according to the method for preparing of claim 1, it is characterized in that the used gathering-device of step 3), improve medical textile materials such as using adsorptive pads, medical adhesive-bonded fabric attached to collecting on the aluminium foil except collecting with aluminium foil merely.
5. according to the method for preparing of claim 1, it is characterized in that the electric spinning condition in the step 4) is: extruded velocity is 0.5mL/h～2mL/h, and voltage is 10kV～20kV, and the distance of spinneret to receptor is 5cm～25cm.
6. according to the method for preparing of claim 1, it is characterized in that the drying condition in the step 5) is: room temperature vacuum drying 24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101370331A CN102641520A (en) | 2012-05-07 | 2012-05-07 | Preparation method of biological repair nano dressing containing wood frog skin polypeptide and being used for wounds and burn |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101370331A CN102641520A (en) | 2012-05-07 | 2012-05-07 | Preparation method of biological repair nano dressing containing wood frog skin polypeptide and being used for wounds and burn |
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| CN102641520A true CN102641520A (en) | 2012-08-22 |
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| CN2012101370331A Pending CN102641520A (en) | 2012-05-07 | 2012-05-07 | Preparation method of biological repair nano dressing containing wood frog skin polypeptide and being used for wounds and burn |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103726224A (en) * | 2014-01-13 | 2014-04-16 | 中国人民解放军第三军医大学 | Non-woven film and charged non-woven biological protection mask prepared by same and preparation method |
| CN103835066A (en) * | 2014-03-27 | 2014-06-04 | 吉林大学 | Preparation method for wood frog skin collagen fiber membrane |
| CN109395153A (en) * | 2018-10-29 | 2019-03-01 | 王景硕 | A kind of wound repair materials |
| CN113546207A (en) * | 2021-07-01 | 2021-10-26 | 中南大学湘雅医院 | Preparation method of frog skin burn wound biological dressing |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493719A (en) * | 2003-07-02 | 2004-05-05 | 东华大学 | Spinning solution and its preparation and application |
| CN1837274A (en) * | 2005-03-21 | 2006-09-27 | 中国科学院化学研究所 | Degradable and absorbable polymer nano fibrous membrane materials and preparation process and use thereof |
| CN101172164A (en) * | 2006-11-03 | 2008-05-07 | 中国科学院化学研究所 | Biopolymer nano tunica fibrosa material capable of being biological degraded and absorbed, preparing method and uses of the same |
| CN101235155A (en) * | 2007-10-31 | 2008-08-06 | 江南大学 | Method for preparing polylactic acid, polyvinyl alcohol and brazilwood mixed nano or micron fibrous membrane |
| CN101491689A (en) * | 2009-03-03 | 2009-07-29 | 沈阳师范大学 | Biodegradable composite superfine fiber medicine controlled-release felt and preparation technique thereof |
| CN101591818A (en) * | 2009-03-10 | 2009-12-02 | 东华大学 | Shikonin PCL/PTMC composite nano fiber and preparation and application |
| CN102068339A (en) * | 2010-12-03 | 2011-05-25 | 北京化工大学 | Preparation method of biodegradable nanofiber medical dressing loaded with medicine |
| CN102397580A (en) * | 2011-11-04 | 2012-04-04 | 无锡中科光远生物材料有限公司 | Anti-adhesion fiber membrane with hemostasis and antibiosis functions and preparation method thereof |
-
2012
- 2012-05-07 CN CN2012101370331A patent/CN102641520A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493719A (en) * | 2003-07-02 | 2004-05-05 | 东华大学 | Spinning solution and its preparation and application |
| CN1837274A (en) * | 2005-03-21 | 2006-09-27 | 中国科学院化学研究所 | Degradable and absorbable polymer nano fibrous membrane materials and preparation process and use thereof |
| CN101172164A (en) * | 2006-11-03 | 2008-05-07 | 中国科学院化学研究所 | Biopolymer nano tunica fibrosa material capable of being biological degraded and absorbed, preparing method and uses of the same |
| CN101235155A (en) * | 2007-10-31 | 2008-08-06 | 江南大学 | Method for preparing polylactic acid, polyvinyl alcohol and brazilwood mixed nano or micron fibrous membrane |
| CN101491689A (en) * | 2009-03-03 | 2009-07-29 | 沈阳师范大学 | Biodegradable composite superfine fiber medicine controlled-release felt and preparation technique thereof |
| CN101591818A (en) * | 2009-03-10 | 2009-12-02 | 东华大学 | Shikonin PCL/PTMC composite nano fiber and preparation and application |
| CN102068339A (en) * | 2010-12-03 | 2011-05-25 | 北京化工大学 | Preparation method of biodegradable nanofiber medical dressing loaded with medicine |
| CN102397580A (en) * | 2011-11-04 | 2012-04-04 | 无锡中科光远生物材料有限公司 | Anti-adhesion fiber membrane with hemostasis and antibiosis functions and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 周涌 等: "林蛙皮活性多肽对小鼠皮肤成纤维细胞增殖的影响", 《中国老年学杂志》, vol. 28, no. 22, 30 November 2008 (2008-11-30) * |
| 姜丽丽等: "中国林蛙皮肤抗菌肽抗菌的特性", 《动物学杂志》, vol. 39, no. 6, 31 December 2004 (2004-12-31) * |
| 牛艳秋等: "中国林蛙药理作用的研究概况", 《中国药师》, vol. 13, no. 4, 30 April 2010 (2010-04-30) * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103726224A (en) * | 2014-01-13 | 2014-04-16 | 中国人民解放军第三军医大学 | Non-woven film and charged non-woven biological protection mask prepared by same and preparation method |
| CN103835066A (en) * | 2014-03-27 | 2014-06-04 | 吉林大学 | Preparation method for wood frog skin collagen fiber membrane |
| CN103835066B (en) * | 2014-03-27 | 2016-03-16 | 吉林大学 | A kind of preparation method of tree frog skin collagen fabric film |
| CN109395153A (en) * | 2018-10-29 | 2019-03-01 | 王景硕 | A kind of wound repair materials |
| CN113546207A (en) * | 2021-07-01 | 2021-10-26 | 中南大学湘雅医院 | Preparation method of frog skin burn wound biological dressing |
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Application publication date: 20120822 |