CN102640873A - Feed for producing selenium-rich storage-resistance green-shell eggs and enhancing chicken body antioxidation capability - Google Patents
Feed for producing selenium-rich storage-resistance green-shell eggs and enhancing chicken body antioxidation capability Download PDFInfo
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Abstract
The invention belongs to the technical field of feed production and provides a feed for producing selenium-rich storage-resistance green-shell eggs and enhancing the chicken body antioxidation capability. The feed consists of basic ration and yeast selenium with the selenium content being 2000 mg/kg, wherein 0.25 to 0.50mg/kg of selenium is added into the basic ration in a yeast selenium form in the basic ration, the Haugh unit reduction in the storage process of egg products can be obviously delayed, in addition, the fresh keeping time of the fresh eggs below the normal temperature of 25 DEG C can be prolonged by more than 15 days; 0.25 to 0.50mg/kg of selenium is added into the basic ration in a yeast selenium form, so the yolk selenium content is improved by 49.15 to 110 percent, the content of 0.437mug/g to 0.637mug/g can be reached, and the chicken body antioxidation index is obviously enhanced; the influence of the selenium on the green-shell hen production performance, the egg quality, the yolk selenium content and the blood serum antioxidation capability is analyzed, the addition of the selenium in the basic ration of the hen is optimized, and the scientific basis is provided for the application of the selenium to the egg poultry culture and the production and the development of the selenium-rich eggs.
Description
Technical field
The invention belongs to the Feed Manufacturing technical field, relate in particular to a kind of feed of producing the rich selenium egg with green shell of anti-the storage and strengthening chicken body oxidation resistance.
Background technology
Selenium is the essential trace element of humans and animals; It with regulate growth, improve immunity of organisms, anticancer, anti-oxidant, anti-stress, delay senility and prevent and treat endemic disease etc. confidential relation is arranged; Humans and animals is generally on the low side to the intake of selenium; And in the human body storage selenium ability very a little less than, often need ediblely contain the higher food of selenium and could obtain enough selenium, so it is significant to develop the food that is rich in selenium.
Se-enriched yeast is the Biological Selenium preparation of a kind of high Se content of microbial conversion, compares with inorganic selenium, and the active high toxicity of selenium is low, has higher-security, is present more satisfactory organic selenium tonic.The early-stage Study of Chinese scholars shows that in the laying hen diet, adding selenium has effects such as production performance, antioxidant ability of organism and the egg product matter of raising.Not only nutrient categories is abundant but also balance is reasonable for egg, and biological value is high, and Se content adds the organic selenium that has than high usage and improve the egg selenium content up to 220 μ g/kg in the laying hen diet, is a kind of relatively benefit selenium method of safety.
Summary of the invention
The invention provides a kind of feed of producing the rich selenium egg with green shell of anti-the storage and strengthening chicken body oxidation resistance, be intended to solve a kind of problem that is used to produce the rich selenium egg with green shell of anti-the storage and improves layer of green-shell egg antioxidant ability of organism feed of present shortage.
The object of the present invention is to provide a kind of feed of producing the rich selenium egg with green shell of anti-the storage and strengthening chicken body oxidation resistance, this feed comprises: Se content is that yeast selenium and the basal diet of 2000mg/kg formed.
Further, the content of corn is 63% in the said basal diet, big soybean meal content is 14%, wheat bran content is 4.5%, premix content is 2.5%, content of stone powder is 8%, the peptide cellulose content is 8%.
Further, said premix is that every kilogram of basal diet provides the VA of 3KIU, the VD of 2.4KIU
3, the VE of 16IU, the VK of 2mg
3, 1.6mg VB
1, 6.5mg VB
2, 3.25mg VB
6, 15 μ g VB
12, the nicotinic acid of 28mg, the pantothenic acid of 8mg, the folic acid of 0.8mg, the Choline Chloride of 475mg, the biotin of 75 μ g, the iron of 50mg, the copper of 4mg, the zinc of 50mg, the manganese of 80mg, the iodine of 1.3mg.
Further; The content of thick protein is 33%～36% in the said peptide element, contains in the thick protein: the peptide below 80.0% the molecular weight 10000D, 2.0% lysine, 0.5% methionine, 12.0% crude fibre, 5.0% SNSP, 10.0% coarse ash, 12.0% moisture.
Further, the trophic level of said basic diet is: the content of metabolizable energy is 10.58%, and the content of thick protein is 14.68%, and the content of calcium is 3.55%, and the content of available phosphorus is 0.36%.
Further, add selenium 0.25～0.50mg/kg with the yeast selenium form in the said basal diet.
The feed of the rich selenium oxidation resistance of the production provided by the invention egg with green shell of anti-the storage is that yeast selenium and the basal diet of 2000mg/kg formed by Se content; With yeast selenium form interpolation selenium 0.25～0.50mg/kg production performance and the egg product matter of laying hen are had no adverse effects in the basal diet; And can significantly delay the decline of egg product storage process Zhong Hashi unit, and can prolong the fresh keeping time of bright egg under 25 ℃ of normal temperature more than 15 days; Add selenium 0.25～0.50mg/kg with the yeast selenium form in the basal diet and can make the yolk Se content raise 49.15%～110%, reach 0.437 μ g/g～0.637 μ g/g; Add selenium 0.25～0.50mg/kg with the yeast selenium form in the basal diet and can significantly improve main antioxidase SOD of laying hen serum and GSH-Px activity; Reduce MDA content; Significantly improve the body TAC, the result of the test that the anti-oxidant index of chicken body significantly strengthens; Through analyzing the influence of selenium to layer of green-shell egg production performance, egg quality, yolk Se content and serum anti oxidability; The suitable addition of selenium in laying hens essential ration carried out preferably; For application and the production exploitation of Se-enriched egg of selenium in egg fowl is cultured provides scientific basis, have stronger popularization and using value.
Description of drawings
Fig. 1 is that the egg that the embodiment of the invention provides stores column sketch map preceding and the Ha Shi unit of storage after 15 days;
Fig. 2 is the column sketch map of Se content in the yolk of the test that provides of the embodiment of the invention 14 days and 28 days.
The specific embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further specified below in conjunction with accompanying drawing and embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in the qualification invention.
The object of the present invention is to provide a kind of feed of producing the rich selenium egg with green shell of anti-the storage and strengthening chicken body oxidation resistance, this feed comprises: Se content is that yeast selenium and the basal diet of 2000mg/kg formed.
In embodiments of the present invention, the content of corn is 63% in the basal diet, big soybean meal content is 14%, wheat bran content is 4.5%, premix content is 2.5%, content of stone powder is 8%, the peptide cellulose content is 8%.
In embodiments of the present invention, premix is that every kilogram of basal diet provides the VA of 3KIU, the VD of 2.4KIU
3, the VE of 16IU, the VK of 2mg
3, 1.6mg VB
1, 6.5mg VB
2, 3.25mg VB
6, 15 μ g VB
12, the nicotinic acid of 28mg, the pantothenic acid of 8mg, the folic acid of 0.8mg, the Choline Chloride of 475mg, the biotin of 75 μ g, the iron of 50mg, the copper of 4mg, the zinc of 50mg, the manganese of 80mg, the iodine of 1.3mg.
In embodiments of the present invention; The content of thick protein is 33%～36% in the peptide element, contains in the thick protein: the peptide below 80.0% the molecular weight 10000D, 2.0% lysine, 0.5% methionine, 12.0% crude fibre, 5.0% SNSP, 10.0% coarse ash, 12.0% moisture.
In embodiments of the present invention, the trophic level of basic diet is: the content of metabolizable energy is 10.58%, and the content of thick protein is 14.68%, and the content of calcium is 3.55%, and the content of available phosphorus is 0.36%.
In embodiments of the present invention; With yeast selenium form interpolation selenium 0.25～0.50mg/kg production performance and the egg product matter of laying hen are had no adverse effects in the basal diet; And can significantly delay the decline of egg product storage process Zhong Hashi unit, and can prolong the fresh keeping time of bright egg under 25 ℃ of normal temperature more than 15 days; Add selenium 0.25～0.50mg/kg with the yeast selenium form in the diet of basis and can make Se content rising 49.15%～110% in the yolk, reach 0.437 μ g/g～0.637 μ g/g.
Below in conjunction with accompanying drawing and specific embodiment application principle of the present invention is further described.
Test material and daily ration are formed: Se content is the yeast selenium of 2000mg/kg; With reference to the nutritional need test preparation basal diet of NRC (1998) laying hen, basal diet is formed and trophic level is seen table 1.
Table 1 basal diet is formed and trophic level
Premix is that every kilogram of daily ration provides: VA 3KIU, VD
32.4KIU, VE 16IU, VK
32mg, VB
11.6mg, VB
26.5mg, VB
63.25mg, VB
1215 μ g, nicotinic acid 28mg, pantothenic acid 8mg, folic acid 0.8mg, Choline Chloride 475mg, biotin 75 μ g, Fe 50mg, Cu 4mg, Zn 50mg, Mn 80mg, I 1.3mg; ) trophic level is calculated value.
Experimental design and feeding and management: select for use 44 age in week 270 of healthy Dongxiang black-feather layer of green-shell egg, will try chicken at random and be divided into 3 groups, every group of 5 repetitions, each repeats 18 chickens, preliminary trial period 7d is just trying phase 28d.Adopt the design of single-factor completely random, 1 group is control group, the basal diet of feeding, and 2,3 groups are added Se 0.25,0.50mg/kg with the SY form respectively in basal diet.
The 3 layers of ladder in test laying hen branch upper, middle and lower are raised in cages, and respectively repeat to be uniformly distributed in each layer of hen house same column, and the hen house temperature is 20～25 ℃, and relative humidity is 75%～85%.Every chicken average every day of feeding 75g freely drinks water, and natural lighting combines with artificial lighting, and every day, light application time was 16h, and gravity-flow ventilation combines with horizontal exhaust ventilation.Clear up chicken manure every day and pick up egg each 2 times, carry out chicken immunity and hurdle house sterilization by conventional program.
The laying hen production performance: duration of test every day is unit statistical average egg size, laying rate, qualified egg rate, death rate and feedstuff-egg ratio by each duplicate record lay eggs sum, total egg size, soft broken egg number and the dead number of washing in a pan with the group.
Egg quality: 20 pieces of eggs of every group of picked at random of experimental period 28d, each repeats 4 pieces.Get 10 pieces of eggs for every group and be used to measure egg size, egg shape index, shell thickness, yolk index (yolk height/yolk width), yolk color (Luo Shi colorimetric fan) and albumen height (albumen elevation measurement appearance).Calculate Ha Shi unit, HU=100 * lg (H-1.7 * W
0.37+ 7.6), wherein H is albumen height (mm), and W is egg size (g).From every group, get 10 pieces of eggs in addition and after normal temperature is stored 15d down, measure the egg size and the albumen height of egg once more, calculate Ha Shi unit.
The yolk Determination of Selenium Contents: experimental period the 14th, 28d extract 10 pieces of eggs from every group (repeating 2 pieces) respectively, adopt mobile injection of hydride generation aas determination yolk Se content (with bright appearance basic calculation).
Superoxide dismutase in the serum (SOD), glutathione peroxidase (GSH-Px), TAC (T-AOC) and MDA (MDA) Determination on content: experimental period 28d 6:00 in morning (on an empty stomach); 10 chickens of every group of picked at random; Each repeats 2 wing venous blood collection 3mL; Leave standstill, centrifuging and taking serum ,-20 ℃ of preservations.Above index all adopts Nanjing to build up the kit detection that bio-engineering research is produced.
Data statistic analysis: the result representes with mean+SD, carries out one-way analysis of variance with SPSS 13.0 statistical softwares, carries out multiple ratio with the LSD method.
Yeast selenium (SY) is to the influence of laying hen production performance: every chicken average every day of feeding 75g, each is organized death rate and is 0.Can know that by table 2 average egg weight, laying rate, qualified egg rate and the feedstuff-egg ratio of each group all do not have significant difference (P＞0.05).
Table 2 yeast selenium is to the influence of laying hen production performance
Notes: take on the different lowercase alphabet differentials of mark different significantly (P＜0.05) with line data, different capitalizations are represented difference extremely significantly (P＜0.01), and no letter or same letter are represented difference not remarkable (P＞0.05), and following table together.
Yeast selenium (SY) is to the influence of egg product matter: can know that by table 3 egg shape index, shell thickness, yolk color and Ha Shi unit of each group all do not have significant difference (P＞0.05), and all a little higher than 1 group of 2,3 groups of yolk colors, and shell thickness is a little less than 1 group.After normal temperature was stored 15d down, 2,3 groups of egg Ha Shi units reduced seldom, respectively than 1 group high by 13.18% (P＜0.01) and 9.63% (P＜0.01).
Table 3 yeast selenium is to the influence of egg product matter
Yeast selenium (SY) to test the 14th, the influence of the yolk Se content of 28d: can be known that by table 4 bird inlay is fed and contained the selenium daily ration, the yolk Se content obviously rises.Test 14d, 2,3 groups of yolk Se contents increase by 47.35% (P＜0.01) and 110.60% (P＜0.01) than 1 group respectively, and 28d improves 49.15% (P＜0.01) and 117.41% (P＜0.01) than 1 group respectively.Prolong with test period, 2,3 groups of yolk Se contents all have the trend (P＞0.05) of increase.
Table 4 yeast selenium to test the 14th, the influence of Se content in the 28d yolk
Selenium is to the influence of SOD in serum, GSH-Px, T-AOC and MDA level: can know by table 5, with the SY form add Se 0.25,0.50mg/kg all has significantly or utmost point appreciable impact SOD, GSH-Px, T-AOC and MDA level in the serum.2,3 groups of SOD vigor are respectively than 1 group high by 5.54% (P＜0.05) and 12.87% (P＜0.01); The GSH-Px vigor is respectively than 1 group high by 28.72% (P＜0.05) and 70.09% (P＜0.01); T-AOC is respectively than 1 group high by 60.23% (P＜0.01) and 71.67% (P＜0.01), and Content of MDA is respectively than 1 group low 13.24% (P＜0.01) and 19.49% (P＜0.01).
Table 5 yeast selenium is to the influence of SOD in serum, GSH-PX, T-AOC and MDA
Conclusion: 1. with SY (yeast selenium) form interpolation Se 0.25～0.50mg/kg production performance and the egg product matter of laying hen are had no adverse effects (P＞0.05) in the diet, and can significantly delay the decline (P＜0.01) of egg product storage process Zhong Hashi unit.Because it is identical that the bright egg of test group is preserved 15 days the bright egg Ha Shi unit of Ha Shi unit and control group the 1st day at normal temperatures, so can prolong the bright egg fresh keeping time of (25 ℃) more than 15 days at normal temperatures; 2. add Se 0.25～0.50mg/kg with SY (yeast selenium) form in the diet and can make yolk Se content rising 49.15%～110% (P＜0.01), reach 0.437 μ g/g～0.637 μ g/g; 3. add Se 0.25～0.50mg/kg with SY (yeast selenium) form in the diet and can significantly improve main antioxidase SOD of laying hen serum and GSH-Px active (P＜0.05), reduce MDA content (P＜0.01), significantly improve body TAC (P＜0.01).
Shown in Fig. 1-2, the feed of the rich selenium oxidation resistance of production that the embodiment of the invention the provides egg with green shell of anti-the storage is that yeast selenium and the basal diet of 2000mg/kg formed by Se content; With yeast selenium form interpolation selenium 0.25～0.50mg/kg production performance and the egg product matter of laying hen are had no adverse effects in the basal diet; And can significantly delay the decline of egg product storage process Zhong Hashi unit, and can prolong the fresh keeping time of bright egg under 25 ℃ of normal temperature more than 15 days; Add selenium 0.25～0.50mg/kg with the yeast selenium form in the basal diet and can make the yolk Se content raise 49.15%～110%, reach 0.437 μ g/g～0.637 μ g/g; Add selenium 0.25～0.50mg/kg with the yeast selenium form in the basal diet and can significantly improve main antioxidase SOD of laying hen serum and GSH-Px activity, reduce MDA content, significantly improve the body TAC; Through analyzing the influence of selenium to layer of green-shell egg production performance, egg quality, yolk Se content and serum anti oxidability; The suitable addition of selenium in laying hens essential ration carried out preferably; For application and the production exploitation of Se-enriched egg of selenium in egg fowl is cultured provides scientific basis, have stronger popularization and using value.
More than be merely preferred embodiment of the present invention,, all any modifications of within spirit of the present invention and principle, being done, be equal to and replace and improvement etc., all should be included within protection scope of the present invention not in order to restriction the present invention.
Claims (6)
1. feed of producing the rich selenium egg with green shell of anti-the storage and strengthening chicken body oxidation resistance is characterized in that this feed comprises: Se content is that yeast selenium and the basal diet of 2000mg/kg formed.
2. feed as claimed in claim 1 is characterized in that, the content of corn is 63% in the said basal diet, big soybean meal content is 14%, wheat bran content is 4.5%, premix content is 2.5%, content of stone powder is 8%, the peptide cellulose content is 8%.
3. feed as claimed in claim 2 is characterized in that, said premix is that every kilogram of basal diet provides the VA of 3KIU, the VD of 2.4KIU
3, the VE of 16IU, the VK of 2mg
3, 1.6mg VB
1, 6.5mg VB
2, 3.25mg VB
6, 15 μ g VB
12, the nicotinic acid of 28mg, the pantothenic acid of 8mg, the folic acid of 0.8mg, the Choline Chloride of 475mg, the biotin of 75 μ g, the iron of 50mg, the copper of 4mg, the zinc of 50mg, the manganese of 80mg, the iodine of 1.3mg.
4. feed as claimed in claim 2; It is characterized in that; The content of thick protein is 33%～36% in the said peptide element, contains in the thick protein: the peptide below 80.0% the molecular weight 10000D, 2.0% lysine, 0.5% methionine, 12.0% crude fibre, 5.0% SNSP, 10.0% coarse ash, 12.0% moisture.
5. according to claim 1 or claim 2 feed, it is characterized in that the trophic level of said basic diet is: the content of metabolizable energy is 10.58%, and the content of thick protein is 14.68%, and the content of calcium is 3.55%, and the content of available phosphorus is 0.36%.
6. feed as claimed in claim 1 is characterized in that, adds selenium 0.25～0.50mg/kg with the yeast selenium form in the said basal diet.
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| CN103005207A (en) * | 2012-12-21 | 2013-04-03 | 灵璧县林汇家禽育种有限责任公司 | Layer feed containing NANO-Se |
| CN103355540A (en) * | 2013-07-23 | 2013-10-23 | 长治市富民饲料科技有限公司 | Laying hen feed capable of prolonging shelf life of eggs |
| CN103493986A (en) * | 2013-10-24 | 2014-01-08 | 湖南农业大学 | Application of selenium yeast or nano selenium used for producing selenium-rich quail eggs capable of achieving long-term preservation |
| CN103598479A (en) * | 2013-11-19 | 2014-02-26 | 界首市德广牧业有限公司 | Feed for feeding laying hens capable of laying green-shell eggs |
| CN103689251A (en) * | 2013-11-26 | 2014-04-02 | 淮北正洋生物科技有限公司 | Selenium-rich feed for laying hens |
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| CN104171427A (en) * | 2014-08-12 | 2014-12-03 | 武汉鼎沃生物科技有限公司 | Chicken feed additive for producing selenium-rich eggs and application of chicken feed additive |
| CN105707500A (en) * | 2015-09-18 | 2016-06-29 | 怀宁县吉丰家庭农场 | Feed for egg-laying hens capable of producing eggs with green eggshells |
| CN107668329A (en) * | 2016-08-02 | 2018-02-09 | 安琪酵母股份有限公司 | Fodder compound, preparation method, application and the method for producing selenium rich of egg |
| CN109953217A (en) * | 2017-12-23 | 2019-07-02 | 惠州市乐夫农业科技有限公司 | Selenium-rich function feed |
| CN110122701A (en) * | 2019-06-03 | 2019-08-16 | 乌海市巴音宝养殖发展有限公司 | A kind of cultural method and its feed of environmental protection selenium-rich laying hen |
| CN110236024A (en) * | 2019-07-24 | 2019-09-17 | 山东天聚安普瑞农牧发展有限公司 | A kind of laying hen produces the feed of laying high selenium eggs |
| CN111528366A (en) * | 2020-06-24 | 2020-08-14 | 郑红飞 | Chicken feed for producing selenium-rich Chinese angelica eggs |
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| CN103005207A (en) * | 2012-12-21 | 2013-04-03 | 灵璧县林汇家禽育种有限责任公司 | Layer feed containing NANO-Se |
| CN104067982A (en) * | 2013-03-27 | 2014-10-01 | 河北天寅生物技术有限公司 | Production method of selenium-rich chicken eggs |
| CN103355540A (en) * | 2013-07-23 | 2013-10-23 | 长治市富民饲料科技有限公司 | Laying hen feed capable of prolonging shelf life of eggs |
| CN103493986A (en) * | 2013-10-24 | 2014-01-08 | 湖南农业大学 | Application of selenium yeast or nano selenium used for producing selenium-rich quail eggs capable of achieving long-term preservation |
| CN103598479A (en) * | 2013-11-19 | 2014-02-26 | 界首市德广牧业有限公司 | Feed for feeding laying hens capable of laying green-shell eggs |
| CN103689251A (en) * | 2013-11-26 | 2014-04-02 | 淮北正洋生物科技有限公司 | Selenium-rich feed for laying hens |
| CN104171427A (en) * | 2014-08-12 | 2014-12-03 | 武汉鼎沃生物科技有限公司 | Chicken feed additive for producing selenium-rich eggs and application of chicken feed additive |
| CN105707500A (en) * | 2015-09-18 | 2016-06-29 | 怀宁县吉丰家庭农场 | Feed for egg-laying hens capable of producing eggs with green eggshells |
| CN107668329A (en) * | 2016-08-02 | 2018-02-09 | 安琪酵母股份有限公司 | Fodder compound, preparation method, application and the method for producing selenium rich of egg |
| CN109953217A (en) * | 2017-12-23 | 2019-07-02 | 惠州市乐夫农业科技有限公司 | Selenium-rich function feed |
| CN110122701A (en) * | 2019-06-03 | 2019-08-16 | 乌海市巴音宝养殖发展有限公司 | A kind of cultural method and its feed of environmental protection selenium-rich laying hen |
| CN110236024A (en) * | 2019-07-24 | 2019-09-17 | 山东天聚安普瑞农牧发展有限公司 | A kind of laying hen produces the feed of laying high selenium eggs |
| CN111528366A (en) * | 2020-06-24 | 2020-08-14 | 郑红飞 | Chicken feed for producing selenium-rich Chinese angelica eggs |
| CN112088973A (en) * | 2020-10-21 | 2020-12-18 | 安徽佰奥生物科技有限公司 | Antibiotic-free laying hen feed |
| CN114376108A (en) * | 2022-03-07 | 2022-04-22 | 湖南省鹏晖农牧有限公司 | Feed and feed additive for improving egg quality |
| CN115553399A (en) * | 2022-11-15 | 2023-01-03 | 江西省农业科学院农产品质量安全与标准研究所 | Feed for egg and meat dual-purpose chicken and using method thereof |
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Address after: 410128 College of animal science and technology, Hunan Agricultural University, Furong district, Changsha, Hunan Applicant after: Hunan Agricultural University Applicant after: Qu Xiangyong Address before: 410128 College of animal science and technology, Hunan Agricultural University, Furong district, Changsha, Hunan Applicant before: Qu Xiangyong Applicant before: Hunan Agricultural University |
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Application publication date: 20120822 |