CN102613578A - Method for preparing food functional products containing high-concentration Gamma-aminobutyric acid - Google Patents
Method for preparing food functional products containing high-concentration Gamma-aminobutyric acid Download PDFInfo
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- CN102613578A CN102613578A CN2012101164014A CN201210116401A CN102613578A CN 102613578 A CN102613578 A CN 102613578A CN 2012101164014 A CN2012101164014 A CN 2012101164014A CN 201210116401 A CN201210116401 A CN 201210116401A CN 102613578 A CN102613578 A CN 102613578A
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Abstract
The invention discloses a method for preparing food functional products containing high-concentration Gamma-aminobutyric acid. The method is characterized by comprising the following steps that: (1) selected beans are cleaned by clean water, impurities and dust are removed, 5 to 10 times of water is used, the beans are subjected to gemmation for 6 to 24 hours in a constant-temperature culture box under the condition of 25 to 50 DEG C, and the pulping is carried out; (2) pulp obtained in the step (1) is inoculated into monosodium glutamate solution with the pH being 4.5 to 7.0 and is placed into an ordinary fermentation tank with a stirring function to be mixed; the weight ratio of the pulp to the monosodium glutamate containing solution is 1:1-5:1 (w/w); and then, the biotransformation is carried out for 0.5 to 8.0 hours under the temperature condition being 20 to 50 DEG C, and biotransformation fermentation liquid is obtained; and (3) the steps of sterilization, sealing and package are carried out. The method has the advantages that monosodium glutamate is decarboxylated and transformed into the food functional ingredient of Gamma-aminobutyric acid through an enzyme system in burgeoned beans, the functional ingredient in the burgeoned beans is obviously improved, and in addition, the additional values of traditional agricultural products are greatly improved.
Description
Technical field
The present invention relates to the method that a kind of preparation contains the food function property product of high concentration GABA.
Background technology
GABA (being called for short GABA) is a kind of nonprotein amino acid that is distributed widely in the animals and plants; By glutamic acid through glutamine decarboxylase catalyzed conversion; Being the inhibiting nerve mediator that is present in mammal brain, the spinal cord, is a kind of functional components that merits attention very much.It is reported; GABA has and brings high blood pressure down, trophic nerve, improves liver kidney function, improves physiological functions such as brain vigor (referring to food industry science and technology; 2003,24 (1) phases, 109～110 pages; And regulate hormone secretion, improve climacteric metancholia, promote alcohol metabolism, disappear smelly and effect such as high-efficient fat reducing GABA (GABA)-a kind of novel functional food factor).Yet the content of GABA in plant tissue quite low (0.3l～20.62 mg/100 g), therefore, the research focus of the current functional food of rich GABA food research and development becoming.Though the chemical method of multiple synthetic GABA is arranged at present, but these methods all receive the restriction of harsh reaction condition and expensive natural material, and in the chemical synthesis product toxic component remove more complicated, and cost is high, poor stability.
Biotransformation method is to produce one of fragrance material three big methods, and than natural extract and chemical synthesis, biotransformation method has special advantages---technology is simple, mild condition, environmentally friendly.Therefore, how to use bioconversion method to obtain colory food function property composition efficiently and become the current research focus.Therefore, to prepare GABA be a valid approach to biological enzyme.
With regard to the enzyme source, comprise two kinds of approach: the first, microbial fermentation and plant tissue metabolism.Microbe fermentation method is to be raw material with glutamic acid or derivatives thereof (sodium glutamate or be rich in material of glutamic acid etc.), utilizes microbial fermentation to make.It is low that its product has a cost, and content is high and can be used safely in the advantage of food.Research about microorganism glutamate decarboxylase (GAD) focuses mostly at Escherichia coli GAD.The microorganism fungus kind of food securities such as saccharomycete, lactic acid bacteria and Aspergillus is difficult usually efficiently obtains.The second, the plant tissue metabolism mainly utilizes endogenous enzymes to transform preparation GABA.And endogenous enzymes conversion preparation GABA has 2 kinds of forms: plant tissue growth metabolism and stripped plant tissue stress metabolism.
The raw material of having reported at present that passes through plant tissue metabolism production GABA has sprouted unpolished rice, germinated soybean, germination maize, germination broad bean, germination cowpea etc.Its technical process is: in the process of germinateing, constantly add the sodium glutamate extract, then germinated rice, germination beans are broken into slurry, obtain containing the functional food product of GABA.Owing to be in the process of germinateing, constantly to add the sodium glutamate extract; Therefore processing technology is complicated the time; And the sodium glutamate extract that adds can not be penetrated in the raw material fully, causes the waste of extract, and the concentration of the GABA that contains in the product is reduced.According to analysis, according to the product that contains GABA that this method makes, with soybean material, the content that the product that per 100 gram soybean siccatives make contains GABA is merely about 100mg～300mg, and content is low.
Summary of the invention
The objective of the invention is to overcome the shortcoming that prior art exists, and provide a kind of be the method that biological Preparation of Catalyst contains the food function property product of high concentration GABA with the germination beans.This food function property product that contains the high concentration GABA is a raw material with the beans that germinates, and being aided with sodium glutamate is transforming agent, carries out bio-transformation under given conditions and processes.It is joined in the food product, have obviously and bring high blood pressure down, trophic nerve, improve liver kidney function, improve physiological functions such as brain vigor.
The technical measures that the present invention takes are: a kind of preparation contains the method for the food function property product of high concentration GABA; The enzyme system that in germination process, produces by beans becomes GABA with sodium glutamate decarboxylation bio-transformation wherein; Be characterized in, comprise the steps:
(1), the step of bean germination and slurrying:
The beans that selects is cleaned with clear water, is removed impurity and dust, with 5～10 times of water 25～50
oIn constant incubator, germinateed slurrying under the C condition 6～24 hours;
(2), the step of fermentation:
Earlier the slurries of step (1) gained being inoculated pH is in 4.5～7.0 the monosodium glutamate solution, and the routine fermentation in-tank mixing that places band to stir; Said slurries are 1:1～5:1 (w/w) with containing the monosodium glutamate solution weight ratio;
Then 20
oC～50
oBio-transformation is 0.5～8.0 hour under the temperature of C, obtains the zymotic fluid of bio-transformation;
(3), the seal-packed step of sterilization:
The zymotic fluid of step (2) gained is packed through sterilization, promptly obtain the food function property product that contains the high concentration GABA of the present invention.
In the said method, the described slurrying of step (1) comprises the beans that germinates is pulverized the method that breaks into homogenate.
In the said method, homogenate rear slurry temperature is no more than 60
oC.
In the said method, the described slurrying of step (1) comprises that with the beans that germinates in freezing under 0～-70 ℃ the temperature, vacuum drying under 0～-70 ℃ temperature, be ground into pulvis again, the water that adds 5 ~ 20 times during use again stirs into homogenate.
In the said method, the described beans of step (1) comprises that various pulse families carry one or more mixing in the edible seed of cultivating thing.
In the said method, the described monosodium glutamate solution of step (2) is that a kind of to contain amount of glutamic acid in mass concentration be 0.1～2% the aqueous solution or extract.
In the said method, the sodium glutamate extract of described sodium glutamate extract for from natural nutraceutical, extracting comprises one or more mixing in bacterium mushroom extracting solution, dried scallop juice, yeast extract, fish meal and the fish sauce that contains high glutamic acid.
In the said method, the described sodium glutamate aqueous solution is the formulated solution of commercially available monosodium glutamate.
In the said method, described step (1) also comprises the beans after germinateing 4 with step (1)
o0.5～36 hour process of precooling in the environment of C～8 ℃.
In the said method, in slurries, add the sodium glutamate extract or the sodium glutamate aqueous solution in the described step (2) after, also comprise add again one thousandth～ten thousand/ calcium chloride or Cobastab
6
Because the present invention has adopted above-mentioned technical scheme, the food function property product that contains the high concentration GABA of its gained (the plain soya-bean milk liquid product of high GABA content plumule) has the following characteristic:
1, proterties: the slight thickness pulpous state of outward appearance liquid, pH are 4.5～7.0 the aqueous solution;
2, fragrance: have the distinctive fragrance of beans;
3, fragrance component and relative ratio of components: the GABA content that detects in the soya-bean milk (slurries) through high performance liquid chromatography is the 200mg/100g soya-bean milk;
4, well-known, mung bean, red bean, black soya bean are as a kind of cereal crops, and itself is as safe as a house; And possess the storage of being prone to, short, enzyme activity advantages of higher of germination cycle, but very low owing to itself containing GABA content, can not give prominence to its effect as functional food ingredient; And up to the present; Do not see the report about bio-transformation enrichment GABA such as germination mung beans, the present invention has carried out the science improvement to the influence factor of germination beans biotransformation method enrichment GABA, makes these mung beans, red bean, black soya bean also can be used as high-quality and low-cost enzyme source; And increased substantially the wherein content of GABA, thereby promoted the development of Chinese food health industry;
5, for preventing that the production operation flow process from the beans that it is good that germinateed the same day possibly occur and can not use at once, must wait until that beans further germinates under the second genius employable situation, the present invention adopts and can the raw material after the germination be put in 4
oC ~ 8
oUnder the environment of C for example 4
oC ~ 8
oThe refrigerator of C or freezer precooling can stop beans further to germinate again by the activity stabilized of protective enzyme like this;
6, product efficacy: GABA (gamma-aminobutyric acid) is known more than 60 kind of a kind of as in the chemical substance of neurotransmitter, is inhibitory transmitter in the prevailing brain.The cynapse of full brain 1/3 with GABA as mediator.The neuron responsive to GABA concentrates in the brain structures such as thalamus, hypothalamus and occipital ctx especially, and GABA has important function in some pathology psychology that suppress to cause because of nervous activity;
7, the biotransformation method of these article employing does not add artificial synthetic additive, and all raw materials are natural material or nature identical, therefore, can be referred to as the natural nutrition functional food.Every day, invented food 100g can replenish 30～200mgGABA, and people's physical efficiency is alleviated stress preferably, regulated mood, recovered physically and mentally healthy, and shortened multiple function and effects such as time for falling asleep and prolong deep sleep time.
The specific embodiment
One or more mixing in the edible seed of cultivating thing are carried in the present invention with various pulse families germination beans is a raw material, contains glutamic acid solution or extract monosodium glutamate solution through germination, pulverizing slurrying, interpolation, makes through fermentation again.
Below for realizing embodiments of the invention, its purpose is conveniently to understand content of the present invention better, and unrestricted protection scope of the present invention.
Embodiment 1
At first will mix beans (red bean, black soya bean, mung bean) with 5 times of water 50
oThe 6h that in constant incubator, germinates under the C condition grinds the beans that germinates and breaks into homogenate with pulverizer, homogenate rear slurry temperature is no more than 60
oC, slurries are for use;
It is in 4.5～7.0 the monosodium glutamate solution that the slurries of gained are inoculated pH, and the routine fermentation in-tank mixing that places band to stir; Said slurries are 1:1 (w/w) with containing the monosodium glutamate solution weight ratio; Maintain the temperature at 20 then
oAbout C, bio-transformation 8 hours obtains bio-transformation liquid; Wherein said monosodium glutamate solution is that a kind of to contain the sodium glutamate amount in mass concentration be 0.1～2% the aqueous solution, obtains by in natural nutraceutical, extracting;
The bio-transformation liquid (promptly referring to accomplish the conversion fluid of bio-transformation) of above-mentioned gained is carried out post processing; The zymotic fluid that is about to gained carries out sterilization earlier and packs; The slurries that obtain are the food function property product that contains the high concentration GABA of the present invention, also claim: the plain soya-bean milk liquid product of high GABA content plumule.
Embodiment 2
At first will mix beans (red bean, black soya bean, mung bean) with 10 times of water 25
oIn constant incubator, germinateed 24 hours under the C condition.For preventing that the production operation flow process from the beans that it is good that germinateed the same day possibly occur and can not use at once, must wait until that beans further germinates under the second genius employable situation, can the raw material after the germination be put in 4
oUnder the environment of C ~ 8 ℃ for example 4
oThe refrigerator or the freezer precooling of C ~ 8 ℃, like this can protective enzyme activity stabilized also can stop beans further to germinate; The time of precooling can not be above 36 hours.The beans that germinates is ground with pulverizer during use again and break into homogenate, homogenate rear slurry temperature is no more than 60
oC, slurries are for use;
It is in 4.5～7.0 the monosodium glutamate solution that the slurries of gained are inoculated pH, and the routine fermentation in-tank mixing that places band to stir; Said slurries are 5:1 (w/w) with containing the monosodium glutamate solution weight ratio; Maintain the temperature at 50 then
oAbout C, bio-transformation 0.5 hour obtains bio-transformation liquid; Wherein said monosodium glutamate solution is that a kind of to contain amount of glutamic acid in mass concentration be 0.1～2% extract, comprises the single solution of high glutamic acid bacterium mushroom extracting solution, dried scallop juice, yeast extract, fish meal and fish sauce or their mixed solution;
The bio-transformation liquid (promptly referring to accomplish the conversion fluid of bio-transformation) of above-mentioned gained is carried out post processing; The zymotic fluid that is about to gained carries out sterilization earlier and packs; The slurries that obtain are the food function property product that contains the high concentration GABA of the present invention, also claim: the plain soya-bean milk liquid product of high GABA content plumule.
Embodiment 3
At first will mix beans (soybean, red bean, black soya bean, mung bean, French beans) with 8 times of water 40
oIn constant incubator, germinateed 15 hours under the C temperature, 8
oC refrigerator or freezer precooling ground the beans that germinates and break into homogenate about 4 hours with pulverizer, homogenate rear slurry temperature is no more than 60
oC, slurries are for use;
It is in 4.5～7.0 the monosodium glutamate solution that the slurries of gained are inoculated pH, and the routine fermentation in-tank mixing that places band to stir; Said slurries are 3:1 (w/w) with containing the monosodium glutamate solution weight ratio; Other add one thousandth to ten thousand/ CaCl
2(calcium chloride) makes Ca
2+(calcium ion) concentration is 2～12mg/100g, and the routine fermentation in-tank mixing that places band to stir, and places the band stirring to maintain the temperature at 35 then
oAbout C, bio-transformation 1.5 hours obtains bio-transformation liquid; Wherein said sodium glutamate is that a kind of to contain the sodium glutamate amount in mass concentration be 0.1～2% the aqueous solution, for example is the formulated solution of commercially available monosodium glutamate; CaCl
2Be food grade, its adding will help to improve the vigor of enzyme, thereby shorten the bio-transformation time, improve industrial production efficiency greatly;
The bio-transformation liquid (promptly referring to accomplish the conversion fluid of bio-transformation) of above-mentioned gained is carried out post processing; The zymotic fluid that is about to gained carries out sterilization earlier and packs; The slurries that obtain are the food function property product that contains the high concentration GABA of the present invention, also claim: the plain soya-bean milk liquid product of high GABA content plumule.
Embodiment 4
At first will mix beans (red bean, black soya bean, mung bean) with 5～10 times of water 25～50
oIn constant incubator, germinateed under the C condition 6～24 hours, and vacuum drying under freezing under 0～-70 ℃ the temperature, 0～-70 ℃ temperature, be ground into powder again, sealing is kept in Dark Place, and is for use.5 ~ 20 times of water of moisturizing before using stir into homogenate.Homogenate rear slurry temperature is no more than 60
oC, slurries are for use;
Is that 4.5～7.0 the natural extract that contains glutamic acid mixes by weight 1:1～5:1 (w/w) with the slurries of gained by slurries and pH, and other adds one thousandth～ten thousand/food grade CaCl
2For example 5～30 mg make Ca
2+Concentration is 2～12mg/100g, and places in the conventional fermentation tank of band stirring, then in temperature 20～50
oUnder the C, bio-transformation 0.5 hour obtains bio-transformation liquid; Wherein said monosodium glutamate solution be a kind of in mass concentration contain amount of glutamic acid be 0.1～2% contain high glutamic acid bacterium mushroom extracting solution, dried scallop juice, yeast extract, fish meal and fish sauce, comprise the single solution of above-mentioned substance or their mixed solution; CaCl
2Be food grade, its adding will help to improve the vigor of enzyme, thereby shorten the bio-transformation time, improve industrial production efficiency greatly;
The bio-transformation liquid (promptly referring to accomplish the conversion fluid of bio-transformation) of above-mentioned gained is carried out post processing; The zymotic fluid that is about to gained carries out sterilization earlier and packs; The slurries that obtain are the food function property product that contains the high concentration GABA of the present invention, also claim: the plain soya-bean milk liquid product of high GABA content plumule.
Embodiment 5
At first will mix beans (red bean, black soya bean, mung bean, broad bean, pea, cowpea) with 5～10 times of water 25～50
oIn constant incubator, germinateed under the C condition 6～24 hours, and vacuum drying under freezing under 0～-70 ℃ the temperature, 0～-70 ℃ temperature, be ground into pulvis again, the water that adds 5 ~ 20 times during use again stirs into homogenate.Homogenate rear slurry temperature is no more than 60
oC, slurries are for use;
The slurries of gained are that 4.5～7.0 monosodium glutamate solution mixes by weight 1:1～5:1 (w/w) by slurries and pH, and other adds one thousandth to ten thousand/Cobastab
6, and place in the conventional fermentation tank of band stirring, and place in the conventional fermentation tank of band stirring, then in temperature 20～50
oUnder the C, bio-transformation 0.5 hour obtains bio-transformation liquid; Wherein said sodium glutamate is that a kind of to contain the sodium glutamate amount in mass concentration be 0.1～2% the aqueous solution, for example is the extract of above-mentioned described natural nutrient substance or the aqueous solution of monosodium glutamate.Described Cobastab
6Be food grade, its adding will help to improve the vigor of enzyme, thereby shorten the bio-transformation time, improve industrial production efficiency greatly;
The bio-transformation liquid (promptly referring to accomplish the conversion fluid of bio-transformation) of above-mentioned gained is carried out post processing; The zymotic fluid that is about to step (2) gained carries out sterilization earlier and packs; Obtain slurries (promptly obtaining containing the soya-bean milk liquid of GABA active principle), be functional food ingredient of the present invention (full name is the plain soya-bean milk liquid product of high GABA content plumule).
It is high that the food function property product that the present invention makes contains GABA concentration, and through test, per 100 gram soya-bean milk liquid contain GABA concentration and reach about 200mg, have improved the yield of functional product greatly, have improved traditional additional value of farm products.
Claims (10)
1. method for preparing the food function property product that contains the high concentration GABA, the enzyme system that in germination process, produces by beans becomes GABA with sodium glutamate decarboxylation bio-transformation wherein, it is characterized in that, comprises the steps:
(1), the step of bean germination and slurrying:
The beans that selects is cleaned with clear water, is removed impurity and dust, with 5～10 times of water 25～50
oIn constant incubator, germinateed slurrying under the C condition 6～24 hours;
(2), the step of fermentation:
Earlier the slurries of step (1) gained being inoculated pH is in 4.5～7.0 the monosodium glutamate solution, and the routine fermentation in-tank mixing that places band to stir; Said slurries are 1:1～5:1 (w/w) with containing the monosodium glutamate solution weight ratio;
Then 20
oC～50
oBio-transformation is 0.5～8.0 hour under the temperature of C, obtains the zymotic fluid of bio-transformation;
(3), the seal-packed step of sterilization:
The zymotic fluid of step (2) gained is packed through sterilization, promptly obtain the food function property product that contains the high concentration GABA of the present invention.
2. method according to claim 1 is characterized in that, the described slurrying of step (1) comprises the beans that germinates is pulverized the method that breaks into homogenate.
3. method according to claim 2 is characterized in that, homogenate rear slurry temperature is no more than 60
oC.
4. method according to claim 1 is characterized in that, the described slurrying of step (1) comprises the beans that germinates 0～-70
oFreezing under the temperature of C, 0～-70
oVacuum drying under the temperature of C, be ground into pulvis again, the water that adds 5 ~ 20 times during use again stirs into homogenate.
5. method according to claim 1 is characterized in that, the described beans of step (1) comprises that various pulse families carry one or more mixing in the edible seed of cultivating thing.
6. method according to claim 1 is characterized in that, the described monosodium glutamate solution of step (2) is that a kind of to contain amount of glutamic acid in mass concentration be 0.1～2% the aqueous solution or extract.
7. method according to claim 6; It is characterized in that; The sodium glutamate extract of described sodium glutamate extract for from natural nutraceutical, extracting comprises one or more mixing in bacterium mushroom extracting solution, dried scallop juice, yeast extract, fish meal and the fish sauce that contains high glutamic acid.
8. method according to claim 6 is characterized in that, the described sodium glutamate aqueous solution is the formulated solution of commercially available monosodium glutamate.
9. method according to claim 1 is characterized in that, described step (1) also comprises the beans after germinateing 4 with step (1)
oC～8
o0.5～36 hour process of precooling in the environment of C.
10. method according to claim 1 is characterized in that, in slurries, add the sodium glutamate extract or the sodium glutamate aqueous solution in the described step (2) after, also comprise add again one thousandth～ten thousand/ calcium chloride or Cobastab
6
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| CN109699917A (en) * | 2019-02-22 | 2019-05-03 | 江汉大学 | A kind of method that the preparation of multi-cultur es combined ferment is rich in γ-aminobutyric acid soy food product |
| CN113543648A (en) * | 2019-07-08 | 2021-10-22 | 梅有限责任公司 | Method for producing rose bay willow beverage with high gamma-aminobutyric acid content |
| CN114711398A (en) * | 2021-01-04 | 2022-07-08 | 湖南汇湘轩生物科技股份有限公司 | Preparation method of chilli paste rich in gamma-aminobutyric acid and chilli paste |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113543648A (en) * | 2019-07-08 | 2021-10-22 | 梅有限责任公司 | Method for producing rose bay willow beverage with high gamma-aminobutyric acid content |
| CN114711398A (en) * | 2021-01-04 | 2022-07-08 | 湖南汇湘轩生物科技股份有限公司 | Preparation method of chilli paste rich in gamma-aminobutyric acid and chilli paste |
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Application publication date: 20120801 |