CN102613198A - Chitosan oligosaccharide agricultural composition for improving coloration of plants and application thereof and method by utilizing same to improve coloration of plants - Google Patents

Chitosan oligosaccharide agricultural composition for improving coloration of plants and application thereof and method by utilizing same to improve coloration of plants Download PDF

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Publication number
CN102613198A
CN102613198A CN2012100585622A CN201210058562A CN102613198A CN 102613198 A CN102613198 A CN 102613198A CN 2012100585622 A CN2012100585622 A CN 2012100585622A CN 201210058562 A CN201210058562 A CN 201210058562A CN 102613198 A CN102613198 A CN 102613198A
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chitosan oligosaccharide
plant
plants
compositions
improve
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CN102613198B (en
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侯华民
张善学
陈坚
陈泰龙
陈其确
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Hainan Zhengye Biotechnology Co ltd
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Hainan Zhengye Zhongnong Hi Tech Co Ltd
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Abstract

The invention relates to a chitosan oligosaccharide agricultural composition for improving coloration of plants and an application thereof and a method by utilizing the same to improve coloration of plants. The invention particularly provides a chitosan oligosaccharide agricultural composition for improving coloration of plants. The weight percentage of the chitosan oligosaccharide in the agricultural composition is 0.0015 to 0.015 percent and is preferentially 0.0025 percent to 0.01 percent. The chitosan oligosaccharide agricultural composition also comprises other ingredients such as one or multiple varieties of glycine, levulinic acid, glutamic acid, phenylalanine, tyrosine, acetylsalicylic acid, genistein, uniconazole, potassium sulphate, monobasic potassium phosphate, dipotassium phosphate, potassium phosphate, calcium chloride and boric acid. The plants include crops and flowers. An evaluation target of the plant coloration is a harvesting target or an ornamental target of the plant such as seeds, fruits, roots, stems, leaves and flowers.

Description

A kind of painted chitosan oligosaccharide agriculturally useful compositions of plant and uses thereof and method improved
Technical field
The invention belongs to agrochemical field, particularly relate to a kind of painted chitosan oligosaccharide agriculturally useful compositions of plant and uses thereof and method improved.
Background technology
Anthocyan is one of main pigment that constitutes the plant color, is plant secondary metabolism product, is one type of water-soluble natural pigment that extensively is present in the plant, and multicoloured color major parts such as fruit, vegetables, flowers are relevant with it.Anthocyan forms many more, and the color of plant is just more beautiful.In addition; Anthocyan is the antidotal nutritious supplementary pharmaceutical of pure natural, is that mankind nowadays is found effective anti-oxidants, and mostly used pigment is synthetic dyestuff in the food industry at present; The long-term health of using meeting harm people, so natural colouring matter just more and more causes the concern of researcher.Increasing of plant anthocyan; Quality of agricultural product improvement, sightseeing tour etc. all had effective facilitation; So gorgeous fruit, vegetables and the flowers of appearance luster are the main causes of the pleasing and persistent pursuit of consumer, also directly embody its commodity performance always.
Improve the plant method of colouring at present and mainly contain the plant illumination condition of improving, bagging, priming leaf, shop reflective membrane etc., but these methods or receive illumination effect, otherwise workload is big.In addition, spray methyl, ethrel, abscisic acid etc. and also can promote the painted of apple, but, cause reducing enduring store property, shorten shelf life because these plant growth substances can cause the arriving of crop respiratory climacteric.The present invention finds that through the property research to chitosan oligosaccharide and composition thereof chitosan oligosaccharide has special effect to the painted improvement of plant, and not only method is simple, and effect is remarkable.
Summary of the invention
Paintedly be subject to illumination condition for solution improves plant, reason such as workload is big or enduring store property is low, the invention provides a kind of be used to improve painted agriculturally useful compositions of plant and uses thereof and method, comprise chitosan oligosaccharide in this agriculturally useful compositions.
Concrete; The invention provides a kind of painted chitosan oligosaccharide agriculturally useful compositions of plant that is used to improve; The percentage by weight that contains chitosan oligosaccharide in this agriculturally useful compositions is 0.0015%-0.015%, and preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.0025%-0.01%.
Concrete; The invention provides a kind of painted chitosan oligosaccharide agriculturally useful compositions of plant that is used to improve; The percentage by weight that contains chitosan oligosaccharide in this agriculturally useful compositions is 0.0015%-0.015%; Preferably; The percentage by weight that chitosan oligosaccharide accounts for composition is 0.0025%-0.01%, also contains other compositions, and other compositions are one or more in glycine, levulinic acid, glutamic acid, phenyl alanine, network propylhomoserin, acetylsalicylic acid, Genistein, uniconazole P, potassium sulphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate, calcium chloride and the boric acid.Preferably, other compositions are two or more in glycine, levulinic acid, glutamic acid, phenyl alanine, network propylhomoserin, acetylsalicylic acid, Genistein, uniconazole P, potassium sulphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate, calcium chloride and the boric acid.
Preferably, the gross weight of other compositions accounts for the 0.05-5% of composition total weight, and is preferred, accounts for 0.05-1%.
Above-mentioned this agriculturally useful compositions can be processed any liquid preparation and any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is aqua, microemulsion or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or water dispersion granule.
The present invention also provides above-mentioned composition to be used to improve the painted purposes of plant.
Especially the painted purposes of plant concrete, that the present invention also provides above-mentioned composition to be used to improve to be caused by anthocyan.
The present invention also provides and has used above-mentioned agriculturally useful compositions and be used to improve the plant colorize method, it is characterized in that every at a distance from 7-14 days, seedling stage, the vegetative period of plant, bloom preceding and/or bloom after use this agriculturally useful compositions.Preferably whenever at a distance from 10 days, use 0-3 time, the concentration of using again and the concentration of preceding applied once are identical or different again
In the said method, this plant is the crop that any needs improve the plant outward appearance, comprises crop and flowers, preferably from plants such as apple, mango, grape, lichee, cherry, strawberry, red French beans, tomato, orchid, kaffir lilies.
In the said method, the painted evaluation object of said plant is plant organ painted in any stage improve the vegetation period, and these organs are seed, fruit, root, stem, leaf, flower.
In the said method, the method for using is meant any effective common method when agricultural product are used, and includes but not limited to spray-on process, seed dressing method, soil treatment method etc.
Among the present invention, chitosan oligosaccharide is that Glucosamine passes through 1, and the degree of polymerization that the 4-glycosidic bond is formed by connecting is the mixture of the high molecular polymer of 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be through any means preparation, and for example following method: deacetylation is the mixture of the chitosan oligomer of 2-30 at the shitosan more than 70% through the degree of polymerization that methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Perhaps, be the mixture of the chitosan oligomer of 2-30 through from fungal cell wall, extracting the degree of polymerization that obtains chitin and prepare through methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods.Perhaps, through passing through 1 by Glucosamine, the degree of polymerization that the chemosynthesis of 4-glycosidic bond is polymerized is the mixture of polymers of 2-30.Chitosan oligosaccharide is the mixture of the high molecular polymer of various polymerization thing at present, that is to say in the chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization possibly exist all.So no matter prepare through which kind of method; The commercially available prod that perhaps prepares through above-mentioned preparation method, as long as it is that Glucosamine passes through 1, the degree of polymerization that the 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly a chitosan oligosaccharide of the present invention; It can contain acetyl group; But the sugar unit of bonding acetyl group is no more than 40% amount, preferably is no more than 30% amount, especially preferably is no more than 20% amount.Commercially available chitosan oligosaccharide all is the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth basically at present.
The painted variation that is meant that plant was taken place by object on color described in the present invention; Relate to the reason that expires and change; The influence factor that anthocyan is arranged; The influence that also has other compositions, the present invention seeks the content that basic reason that chitosan oligosaccharide changes color has been to change the anthocyan in plant results object or the required object through the content of measuring anthocyan.
Beneficial effect of the present invention:
The present invention has found to be used to improve the painted agriculturally useful compositions that comprises chitosan oligosaccharide of plant; The chitosan oligosaccharide of particularly having found certain concentration is improving the painted special effect of plant; Especially use the agriculturally useful compositions that comprises chitosan oligosaccharide after handling plant, not only improve coloring effect, the commodity performance improves; And can not produce poisoning, non agricultural chemical residuum.The contribution that the present invention is special also is to use chitosan oligosaccharide and painted good chance and the concentration of composition for improved plant through having discovered, has improved the commodity performance of plant greatly.
Embodiment
In order to understand the present invention, further specify the present invention with embodiment below, but do not limit the present invention.
FORMULATION EXAMPLE 1
Get 0.05 gram chitosan oligosaccharide, be diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 2
Get 0.15 gram chitosan oligosaccharide, be diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 3
Get 0.09 gram chitosan oligosaccharide, glycine 2g, boric acid 1g is diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 4
Get 0.075 gram chitosan oligosaccharide, levulinic acid 3g, network propylhomoserin 0.5g is diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 5
Get 0.075 gram chitosan oligosaccharide, glutamic acid 1.5g, acetylsalicylic acid 1.5g is diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 6
Get 0.095 gram chitosan oligosaccharide, phenyl alanine 2g, Genistein 2.5g is diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 7
Get 0.08 gram chitosan oligosaccharide, potassium sulphate 0.5g, potassium dihydrogen phosphate 0.5g, calcium chloride 1.5g is diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 8
Get 0.12 gram chitosan oligosaccharide, uniconazole P 0.2g, potassium phosphate 3.5g is diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 9
Get 0.15 gram chitosan oligosaccharide, dipotassium hydrogen phosphate 3.0g, calcium chloride 1.5g is diluted with water to 1000g, spray application.
FORMULATION EXAMPLE 10
Get 0.15 gram chitosan oligosaccharide, dipotassium hydrogen phosphate 3.0g is diluted with water to 1000g, spray application.
Effect embodiment 1:
Select plants such as apple, mango, grape, lichee, cherry, strawberry, red French beans, tomato, orchid, kaffir lily, with the preparation dilution of FORMULATION EXAMPLE 1-10, what wherein concentration was represented is the valid density of chitosan oligosaccharide, handles as contrast with clear water respectively.The solution of above-mentioned preparation is in the crop of each vegetative stage according to the demand treatment by stages, and every processing is handled more than 20 strains at least.The time interval of handling repeatedly is 10 days.Contrast adopts clear water to handle, and the clear water consumption is identical with the consumption of preparation.Take the different plants organ of chitosan oligosaccharide and compositions-treated at random in processing plant moment; AAS is measured the anthocyanidin content of more different plant organs; The detected value of handling with clear water is defined as 100, and the percentage result that the value of the value of processing and clear water contrast compares sees table 1.
The anthocyanidin content of the different plants of table 1 after chitosan oligosaccharide and compositions-treated thereof
Figure BDA0000141376530000051
Figure BDA0000141376530000061
No matter show through the test range estimation, be single or continuous administration chitosan oligosaccharide and chitosan oligosaccharide composition, and the plant coloring effect improves.Find (seeing table 1) through the AAS colorimetric estimation, with respect to blank, chitosan oligosaccharide and chitosan oligosaccharide composition different disposal plant, the content of anthocyan all improves in the plant organ, and visible, chitosan oligosaccharide and chitosan oligosaccharide composition obviously improve the coloring effect of plant.

Claims (8)

1. one kind is used to improve the painted chitosan oligosaccharide agriculturally useful compositions of plant, and the percentage by weight that contains chitosan oligosaccharide in this agriculturally useful compositions is 0.0015%-0.015%, and preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.0025%-0.01%.
2. one kind is used to improve the painted chitosan oligosaccharide agriculturally useful compositions of plant; The percentage by weight that contains chitosan oligosaccharide in this agriculturally useful compositions is 0.0015%-0.015%; Preferably; The percentage by weight that chitosan oligosaccharide accounts for composition is 0.0025%-0.01%; Also contain other compositions, other compositions are one or more in glycine, levulinic acid, glutamic acid, phenyl alanine, network propylhomoserin, acetylsalicylic acid, Genistein, uniconazole P, potassium sulphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate, calcium chloride and the boric acid.
3. composition according to claim 2, wherein other compositions are two or more in glycine, levulinic acid, glutamic acid, phenyl alanine, network propylhomoserin, acetylsalicylic acid, Genistein, uniconazole P, potassium sulphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate, calcium chloride and the boric acid.
4. according to claim 2 or 3 described compositions, wherein the gross weight of other compositions accounts for the 0.05-5% of composition total weight, and is preferred, accounts for 0.05-1%.
5. each described composition of claim 1-4 is used to improve the painted purposes of plant.
6. each described composition of claim 1-4 is used to improve the painted purposes of plant that is caused by anthocyan.
7. each described composition of claim 1-4 is used to improve the plant colorize method, it is characterized in that every at a distance from 7-14 days, seedling stage, the vegetative period of plant, bloom preceding and/or bloom after use said agriculturally useful compositions.
8. method according to claim 7 is characterized in that whenever at a distance from 10 days, uses 0-3 time again, and the concentration of using again and the concentration of preceding applied once are identical or different.
CN201210058562.2A 2012-03-07 2012-03-07 Chitosan oligosaccharide agricultural composition for improving coloration of plants and application thereof and method by utilizing same to improve coloration of plants Active CN102613198B (en)

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Cited By (7)

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CN104649807A (en) * 2013-11-21 2015-05-27 中国科学院大连化学物理研究所 Chitosan oligosaccharide compound and application of chitosan oligosaccharide compound in relieving albino seedling of graminaceous crops
CN105432343A (en) * 2015-11-11 2016-03-30 长江大学 Method for increasing ginkgo biloba flavonoid content and application of genistein
CN105985179A (en) * 2015-12-29 2016-10-05 芜湖市创源新材料有限公司 Coloring promotion type special fertilizer for grapes
CN107739250A (en) * 2017-11-16 2018-02-27 潍坊友容实业有限公司 Saline and alkaline region orchard functional form liquid fertilizer and preparation method thereof
CN111018600A (en) * 2019-12-15 2020-04-17 惠州学院 Foliar fertilizer for improving anthocyanin content of litchi peels and preparation and use methods thereof
CN111559937A (en) * 2020-05-21 2020-08-21 潍坊真维农生物工程有限公司 Biological activator for promoting fruit coloring and preparation method and application thereof
CN114437250A (en) * 2022-03-15 2022-05-06 内蒙古农业大学 Chitosan oligosaccharide amino hydroxyl propionamide derivative and preparation method thereof

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104649807A (en) * 2013-11-21 2015-05-27 中国科学院大连化学物理研究所 Chitosan oligosaccharide compound and application of chitosan oligosaccharide compound in relieving albino seedling of graminaceous crops
CN105432343A (en) * 2015-11-11 2016-03-30 长江大学 Method for increasing ginkgo biloba flavonoid content and application of genistein
CN105432343B (en) * 2015-11-11 2018-03-23 长江大学 A kind of application for the method and Genistein for improving ginkgo biloba flavonoid content
CN105985179A (en) * 2015-12-29 2016-10-05 芜湖市创源新材料有限公司 Coloring promotion type special fertilizer for grapes
CN107739250A (en) * 2017-11-16 2018-02-27 潍坊友容实业有限公司 Saline and alkaline region orchard functional form liquid fertilizer and preparation method thereof
CN111018600A (en) * 2019-12-15 2020-04-17 惠州学院 Foliar fertilizer for improving anthocyanin content of litchi peels and preparation and use methods thereof
CN111559937A (en) * 2020-05-21 2020-08-21 潍坊真维农生物工程有限公司 Biological activator for promoting fruit coloring and preparation method and application thereof
CN114437250A (en) * 2022-03-15 2022-05-06 内蒙古农业大学 Chitosan oligosaccharide amino hydroxyl propionamide derivative and preparation method thereof

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