CN102604873A - Rejuvenation method of marine luminous bacteria - Google Patents
Rejuvenation method of marine luminous bacteria Download PDFInfo
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- CN102604873A CN102604873A CN2012100939487A CN201210093948A CN102604873A CN 102604873 A CN102604873 A CN 102604873A CN 2012100939487 A CN2012100939487 A CN 2012100939487A CN 201210093948 A CN201210093948 A CN 201210093948A CN 102604873 A CN102604873 A CN 102604873A
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Abstract
The invention relates to a rejuvenation method of marine luminous bacteria. The rejuvenation method comprises the steps of inoculating strains to a rejuvenation medium for subculture, screening the strains according to the luminous intensity, and finally preparing the screened strains into lyophilized powder for verification. The rejuvenation method disclosed by the invention is mainly used for preserving or maintaining performances and fertility of a microorganism, recovering the capacity of preparing the lyophilized powder of the marine luminous bacteria, and regulating the luminous intensity of the marine luminous bacteria.
Description
Technical field
The present invention relates to the rejuvenation method of a kind of ocean photogenic bacterium, this method is mainly used in preservation or keeps performance and the prolificacy of mikrobe, recovers the ocean photogenic bacterium and prepares the ability of lyophilized powder and regulate its luminous intensity.
Background technology
Along with environmental pollution is serious day by day; A large amount of hazardous contaminants is discharged into lake, river and ocean; The eubiosis and organism to aquatic ecosystem work the mischief; Detect advantages such as quick, easy because photogenic bacterium toxotest method has, this method at home and abroad is used widely.Most widely used in the photogenic bacterium toxotest is ocean photogenic bacterium (Photobacteriumphosporum); But owing to going down to posterity repeatedly or reason such as preservation is improper; Cause ocean photogenic bacterium performance degradation; Cause photogenic bacterium because of can't being prepared into inconvenient preservation of lyophilized powder and use, even can not be used for toxotest, therefore need carry out rejuvenation to recover its performance bacterial classification.The cultured method realization repeatedly of its special culture media is adopted in the rejuvenation of bacterial classification usually in the prior art; Adopt the cultured method rejuvenation repeatedly of photobacterium phosphoreum substratum like photobacterium phosphoreum; But when the spawn degeneration degree is serious; This method is also inapplicable, still can't recover its performance even the process number is commissioned to train to support.
In addition; The luminous intensity scope of different types of ocean photogenic bacterium differs bigger, and is stronger such as the luminous intensity of photobacterium phosphoreum, the luminous intensity of Fei Shi vibrios a little less than; And the sensing range of faint light detector is limited, has so just limited applying of some ocean photogenic bacterium.For example, BHP9511 type water quality toxicity rapid detection appearance can detect 0-2000000s
-1The light intensity of scope, the stronger light that sends for photobacterium phosphoreum then can not detect.Therefore be necessary to seek an approach with the luminous intensity of solution photogenic bacterium and the unmatched problem of sensing range of faint light detector.
Summary of the invention
The effect of rejuvenation that the objective of the invention is to solve ocean photogenic bacterium in the prior art is paid no attention to the luminous intensity of thinking of the ocean photogenic bacterium and the unmatched problem of sensing range of some faint light detector, and the novel method of a kind of ocean photogenic bacterium rejuvenation is provided.
For achieving the above object, the invention provides the rejuvenation method of a kind of ocean photogenic bacterium, comprise rejuvenation cultivation, screening and proof procedure, it is characterized in that:
1) prescription of the used rejuvenation cultivation base of rejuvenation cultivation process is ammonium chloride 0.3g/L, lime carbonate 1g/L, Agavain 2-8g/L; Sal epsom 0.3g/L, potassium primary phosphate 3g/L, sodium-chlor 20-30g/L; Sodium Glycerophosphate 20-25g/L, yeast extract paste 2.5g/L, pH7 ± 0.2;
2) during rejuvenation cultivation, bacterial classification inoculation to the cultivation of going down to posterity of rejuvenation cultivation base, and is detected the luminous intensity of bacterium liquid when cultivating 21h going down to posterity at every turn, reach 600000-1000000s until luminous intensity
-1Till;
3) in the screening process, with luminous intensity at 600000-1000000s
-1Between bacterium liquid separate single bacterium colony, and the single bacterium colony of choosing the luminous intensity basically identical of luminous intensity and bacterium liquid is preserved subsequent use;
The mode of 4) adopt making lyophilized powder is verified the bacterial classification of single bacterium colony of being screened.
The above-mentioned detector model that is used to detect luminous intensity is a BHP9511 type water quality toxicity rapid detection appearance.
Rejuvenation method of the present invention has improved prolificacy and the mitotic stability of ocean photogenic bacterium, makes its ability of recovering the preparation lyophilized powder, and the luminous intensity of having regulated the ocean photogenic bacterium, so that be complementary with the sensing range of faint light detector.
Embodiment
Do below in conjunction with embodiment and to further describe.
Embodiment 1: the rejuvenation of Fei Shi vibrios
1) material and equipment:
The Fei Shi vibrios of decline: luminous intensity is 5000-50000s
-1
The rejuvenation cultivation base, its prescription is: ammonium chloride 0.3g/L, lime carbonate 1g/L, Agavain 3g/L, sal epsom 0.3g/L, potassium primary phosphate 3g/L, sodium-chlor 25g/L, Sodium Glycerophosphate 24g/L, yeast extract paste 2.5g/L, pH7 ± 0.2;
BHP9511 type water quality toxicity rapid detection appearance: sensing range is 0-2000000s
-1
2) operation steps:
At first carry out rejuvenation cultivation; Be about to bacterial classification inoculation to the rejuvenation cultivation base, at 23 ℃, the cultivation of going down to posterity under the condition of 130r/min; The time standby BHP9511 type water quality toxicity detector of 21h of at every turn going down to posterity detects the luminous intensity of bacterium liquid, reaches 600000-1000000s until luminous intensity
-1Between till;
Strain screening then, the luminous intensity that is about to choose is at 600000-1000000s
-1Between bacterium liquid be inoculated into solid medium and cultivate, and separate single bacterium colony; Observe the luminous intensity of bacterium colony, choose single bacterium colony of the luminous intensity basically identical of luminous intensity and bacterium liquid and preserve subsequent use;
At last, verify through the mode that selected single bacterium colony is made into lyophilized powder.
Experiment showed, that the Fei Shi vibrios after the rejuvenation can process lyophilized powder, and luminous intensity is at 800000-1100000s
-1Between.
Embodiment 2: the rejuvenation of photobacterium phosphoreum
1) material and equipment:
The photobacterium phosphoreum of decline: culture propagation is slow, luminous intensity 4000000-5000000s
-1
The rejuvenation cultivation base, its prescription is: ammonium chloride 0.3g/L, lime carbonate 1g/L, Agavain 7g/L, sal epsom 0.3g/L, potassium primary phosphate 3g/L, sodium-chlor 30g/L, Sodium Glycerophosphate 21g/L, yeast extract paste 2.5g/L, pH7 ± 0.2;
BHP9511 type water quality toxicity rapid detection appearance: sensing range is 0-2000000s
-1
2) operation steps: to the rejuvenation cultivation base, at 18 ℃, the cultivation of going down to posterity under the condition of 130r/min detects the luminous intensity of bacterium liquid at every turn when going down to posterity 21h, choose luminous intensity at 600000-1000000s with bacterial classification inoculation
-1Between bacterium liquid separate single bacterium colony, observe the luminous intensity of single bacterium colony, choose single bacterium colony of the luminous intensity basically identical of luminous intensity and bacterium liquid, selected single bacterium colony is made into lyophilized powder.
Experiment showed, that the photobacterium phosphoreum after the rejuvenation can process lyophilized powder, and luminous intensity is at 1500000-2000000s
-1Between, the sensing range of the luminous intensity of the photobacterium phosphoreum after the rejuvenation and BHP9511 type water quality toxicity rapid detection appearance is complementary.
Claims (4)
1. the rejuvenation method of an ocean photogenic bacterium comprises rejuvenation cultivation, screening and proof procedure, it is characterized in that:
1) the used rejuvenation cultivation base of rejuvenation cultivation process contains Agavain 2-8g/L, Sodium Glycerophosphate 20-25g/L, yeast extract paste 2.5g/L;
2) during rejuvenation cultivation, bacterial classification inoculation to the cultivation of going down to posterity of rejuvenation cultivation base, and is detected the luminous intensity of bacterium liquid when cultivating 21h going down to posterity at every turn, reach 600000-1000000s until luminous intensity
-1Till;
3) in the screening process, with luminous intensity at 600000-1000000s
-1Between bacterium liquid separate single bacterium colony, and the single bacterium colony of choosing the luminous intensity basically identical of luminous intensity and bacterium liquid is preserved subsequent use.
2. the rejuvenation method of ocean as claimed in claim 1 photogenic bacterium is characterized in that: said rejuvenation cultivation base also contains ammonium chloride 0.3g/L, lime carbonate 1g/L; Sal epsom 0.3g/L; Potassium primary phosphate 3g/L, sodium-chlor 20-30g/L, the pH of said rejuvenation cultivation base are 7 ± 0.2.
3. according to claim 1 or claim 2 the rejuvenation method of ocean photogenic bacterium is characterized in that: adopt the mode of making lyophilized powder that the single bacterium colony that is screened is verified.
4. according to claim 1 or claim 2 the rejuvenation method of ocean photogenic bacterium is characterized in that: the said detector model that is used to detect luminous intensity is a BHP9511 type water quality toxicity rapid detection appearance.
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| CN2012100939487A CN102604873A (en) | 2012-03-28 | 2012-03-28 | Rejuvenation method of marine luminous bacteria |
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| CN2012100939487A CN102604873A (en) | 2012-03-28 | 2012-03-28 | Rejuvenation method of marine luminous bacteria |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979170A (en) * | 2020-07-02 | 2020-11-24 | 山东省临沂生态环境监测中心 | Rejuvenation method of photobacteria for monitoring environmental pollutants |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001006000A1 (en) * | 1999-07-20 | 2001-01-25 | Micrology Laboratories, L.L.C. | Test media for identification and differentiation of enterobacteriaceae |
| CN1076755C (en) * | 1997-01-07 | 2001-12-26 | 华东师范大学 | Qinghai Vibrion dry-powder preparation |
| CN101712936A (en) * | 2008-10-06 | 2010-05-26 | 中国海洋大学 | Detection of environmental pollutants by using Photobacterium leiognathi YL bacterial strain |
| CN1455243B (en) * | 2002-04-29 | 2010-09-08 | 拜尔公司 | Method and kit for detecting biological active matter |
| CN102071159A (en) * | 2009-11-20 | 2011-05-25 | 中国海洋大学 | Detection of environmental pollutants through photobacterium leiognathi |
-
2012
- 2012-03-28 CN CN2012100939487A patent/CN102604873A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076755C (en) * | 1997-01-07 | 2001-12-26 | 华东师范大学 | Qinghai Vibrion dry-powder preparation |
| WO2001006000A1 (en) * | 1999-07-20 | 2001-01-25 | Micrology Laboratories, L.L.C. | Test media for identification and differentiation of enterobacteriaceae |
| CN1455243B (en) * | 2002-04-29 | 2010-09-08 | 拜尔公司 | Method and kit for detecting biological active matter |
| CN101712936A (en) * | 2008-10-06 | 2010-05-26 | 中国海洋大学 | Detection of environmental pollutants by using Photobacterium leiognathi YL bacterial strain |
| CN102071159A (en) * | 2009-11-20 | 2011-05-25 | 中国海洋大学 | Detection of environmental pollutants through photobacterium leiognathi |
Non-Patent Citations (4)
| Title |
|---|
| 方战强等: "发光细菌法在水质监测中的应用", 《重庆环境科学》 * |
| 闫鹏等: "细菌发光传感器对污染物急性毒性快速检测的研究", 《中国卫生工程学》 * |
| 韩润平等: "利用生物毒性仪进行水质监测的探讨", 《遵义师范学院学报》 * |
| 马梅等: "新型淡水发光菌(Vibrio qinghaiensis sp.――Q67)应用于环境样品毒性测试的初步研究", 《环境科学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979170A (en) * | 2020-07-02 | 2020-11-24 | 山东省临沂生态环境监测中心 | Rejuvenation method of photobacteria for monitoring environmental pollutants |
| CN111979170B (en) * | 2020-07-02 | 2022-10-25 | 山东省临沂生态环境监测中心 | Rejuvenation method of photogenic bacteria for monitoring environmental pollutants |
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Address after: 100070, room 3, building 128, No. 903 South Fourth Ring Road, Beijing, Fengtai District Applicant after: Beijing Hamamatsu Photonics Technology Co., Ltd. Address before: 100070, No. 18, building 11, 188, base station, South Fourth Ring Road West, Beijing, Fengtai District Applicant before: Beijing Hamamatsu Photonics Technology Co., Ltd. |
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Application publication date: 20120725 |