CN103937697A - Bacterial strain for degrading dye with high efficiency - Google Patents

Bacterial strain for degrading dye with high efficiency Download PDF

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CN103937697A
CN103937697A CN201410015741.7A CN201410015741A CN103937697A CN 103937697 A CN103937697 A CN 103937697A CN 201410015741 A CN201410015741 A CN 201410015741A CN 103937697 A CN103937697 A CN 103937697A
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bacterial strain
decolouring
dye
dyestuff
lysinibacillus
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CN103937697B (en
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陶然
杨扬
苏萌
赵建成
钟胜强
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Xiamen Zhongren Hemei Biotechnology Co.,Ltd.
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Jinan University
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Abstract

The invention discloses a bacterial strain for degrading dye with high efficiency. The bacterial strain is Lysinibacillussp ((i) Lysinibacillussp.(/i))FS1, and is preserved in the China Center for Type Culture Collection with the preservation number is CCTCC NO: M2013561 on Nov, 10th, 2013. The bacterial strain has the advantage of rapid growth and strong environment adaptability, has dye degradation capability with wide spectrum, and has good decolouring effect to a plurality of dyes, maximum decolourization percentage can be reached by culturing for 10 hours, the percent of decolourization can reach more than 90%, and the maximum decolouring concentration can reach 5000mg/L. The bacterial strain can be used for decolouring the printing and dyeing waste water and correlated dye waste water, can effectively avoid the dye molecules which are difficulty performed with natural degradation to directly enter in the water body for environment pollution, no secondary pollution is generated, and the bacterial strain has good ecology efficiency and application prospect. The Lysinibacillussp can be better used for degrading the dye, fills the blank of domestic technology, and provides a powerful technical base for solving the dye sewage treatment, especially the decolouring problem.

Description

The bacterial strain of one high-efficiency degradation dyestuff
Technical field
The present invention relates to biological technical field, more specifically, relate to the bacterial strain of a high-efficiency degradation dyestuff.
Background technology
Synthetic dyestuff is widely used in the industries such as printing and dyeing, leather, food, makeup, paper product, produces thus a large amount of waste water from dyestuff.Due to waste water from dyestuff, there is the features such as colourity is high, complicated components, chemical stability is strong, biodegradability is poor, in the discharge entered environment of a large amount of dyestuffs along with trade effluent, most azoic dyestuffs have potential three and cause effect (teratogenesis, carcinogenic, mutagenesis), and environment and human health are had to serious harm.Area, China Pearl River Delta dyestuff year usage quantity surpasses 2 * 10 at present 4ton, wherein has 10～20% dyestuffs to be directly discharged in environment, and azo dyes accounts for 50% left and right of total release.
At present the treatment process of waste water from dyestuff is had a lot, chemical method is if flocculence, oxidation style, electrochemical process, Physical are as By Bubble-floating Method, supersonic method, membrane separation process, absorption and extraction etc., although these methods are effective, treatment effect is good, processing cost is higher, easily causes secondary pollution.Although the biological treatment costs such as traditional activated sludge process, membrane biological process are low, non-secondary pollution, because the degradability of dyestuff is poor, cannot thoroughly be degraded by common microorganism, and its application is subject to certain restrictions.
From environment, Isolation and screening goes out microbial strains that can efficient degradation dyestuff, is applied to the processing of waste water from dyestuff, is effective biological treatment, has a good application prospect and ecological benefits.At present to existing Methionin genus bacillus ( lysinibacillus sp.) research mainly concentrate on ability or the degradation capability to persistent organic pollutant of its reducing heavy metal, for example patent CN 102363756A announces a strain Methionin genus bacillus lysinibacillus sp. GY32degraded to polychlorobiphenyl ether BDE209, patent CN103395893A has announced a strain can reduce Cr 6+methionin genus bacillus.And at present domesticly yet there are no Methionin genus bacillus for dye decolored Research Literature and patent report.
Summary of the invention
The technical problem to be solved in the present invention is to fill up the art blank, and the Methionin genus bacillus strain that a strain can efficient degradation dyestuff is provided.
Another technical problem that the present invention will solve is to provide separation method and the cultural method of described bacterial strain.
Object of the present invention is achieved by the following technical programs:
Provide a high-efficiency degradation dyestuff Methionin Bacillus strain ( lysinibacillus sp.) FS1, on November 10th, 2013, being preserved in Chinese Typical Representative culture collection center (address is Hubei China province Wuhan City Luo Jia Shan Wuhan University), deposit number is: CCTCC NO:M 2013561.Be preserved in Chinese Typical Representative culture collection center, deposit number is CCTCC NO:M 2013561.
Described bacterial strain is bacillus, Gram-positive, bacterium colony is circular, smooth surface is flat, neat in edge, faint yellow, opaque, have pearliness, colony diameter 2mm～6mm, the sequence of this bacterial strain 16S rDNA is as shown in SEQ ID NO.1.With lysinibacillus fusiformisnBRC 15717(NCBI accession number AB271743) there is 99% homology.
The bacterial strain FS1 of efficient degradation dyestuff of the present invention is obtaining good effect aspect dyestuff degraded, especially aspect decoloring dye waste water.
The present invention provides the separation method of the bacterial strain FS1 of described efficient degradation dyestuff simultaneously, be by the active sludge sample of collection through bacterial classification enrichment medium enrichment culture to having after obvious decolouring phenomenon, gradient dilution, bacterium liquid after gradient dilution is applied on screening culture medium flat board, cultivate 24～48h, the picking circle that obviously decolours, rules, chooses single bacterium colony continuously in screening culture medium cultivation until after without miscellaneous bacteria for 37 ℃, picking list bacterium colony is in decolouring substratum, in 37 ℃ of standing cultivations and get final product.
The application of the bacterial strain FS1 that the invention provides the efficient degradation dyestuff that described separation method separation obtains aspect dyestuff degraded especially has good effect aspect decoloring dye waste water.
Consisting of of described enrichment medium: extractum carnis 1g, peptone 2g, glucose 3g, K 2hPO 42g, NaH 2pO 40.5g, MgSO 47H 2o 0.2g, MnSO 47H 2o 0.02g, FeCl 37H 2o 0.01g, CaCl 20.02g, sterilized water 1L, pH 7.2;
Consisting of of described screening culture medium: yeast powder 5g, sodium-chlor 5g, peptone 10g, agar 20g, sterilized water 1L, pH7.2;
Consisting of of described decolouring substratum: carbon source 2g, nitrogenous source 1g, NaH 2pO 40.5g, K 2hPO 42g, FeSO 47H 2o 0.01g, MgSO 47H 2o 0.2g, CaCl 20.02g, sterilized water 1L, pH 7.2.
Above substratum all can be at 121 ℃ of sterilizing 15～30min.
Bacterial strain of the present invention is that separation obtains from the active sludge of wastewater treatment aeration tank, industry park, Guangzhou, Guangdong at first.
The cultural method of the bacterial strain FS1 of efficient degradation dyestuff of the present invention, is that described bacterial classification is inoculated in liquid nutrient medium, and at 30～37 ℃, shaking table is cultivated the nutrient solution that 10～24h obtains described bacterial strain;
Consisting of of described liquid nutrient medium: glucose 2g, ammonium chloride 1g, NaH 2pO 40.5g, K 2hPO 42g, FeSO 47H 2o 0.01g, MgSO 47H 2o 0.2g, CaCl 20.02g, water 1L, pH7.2.
Cultural method of the present invention is cultivated the strain cultured solution obtaining and aspect dyestuff degraded, is had good application.Especially aspect decoloring dye waste water, there is good effect.
Bacterial strain of the present invention and nutrient solution thereof relate to multiple dyestuff in the application aspect dye decolored, and special azo dyes wastewater degradation effect is better.
More preferably, the multiple dyestuffs such as tropeolin-D, Acid Red B, toluylene red and/or methylene blue are had to good degradation effect.
Preferably, the concentration range of the dyestuff that described application is the most applicable is 80～5000mg/L, can obtain good decolorizing effect.
Preferably, bacterial strain lysinibacillus sp.fS1 to the decolouring concentration of methylene blue not higher than 4000mg/L.
Preferably, bacterial strain lysinibacillus sp.the red decolouring concentration of FS1 centering is not higher than 2000mg/L.
Preferably, bacterial strain lysinibacillus sp.fS1 to the decolouring concentration of tropeolin-D not higher than 500mg/L.
Preferably, bacterial strain lysinibacillus sp.fS1 to the decolouring concentration of Acid Red B not higher than 5000mg/L.
Preferably, in described application, be that the bacterial strain FS1 of described efficient degradation dyestuff is cultured to logarithmic phase, get nutrient solution, be that 1～5% inoculum size access is decoloured in basic medium by volume, add described dyestuff to carry out normal temperature cultivation, realize decolouring.Preferably, the temperature that described normal temperature is cultivated is 10～40 ℃, pH6～9, incubation time 10～24h.
Preferably, in described application, be by the bacterial strain of efficient degradation dyestuff fS1strain cultured solution be in 1～5% inoculum size access decolouring basic medium by volume, add described dyestuff to carry out normal temperature cultivation, realize decolouring.
Preferably, the temperature that described normal temperature is cultivated is 10～40 ℃, pH6～9, incubation time 10～24h.
Beneficial effect of the present invention:
The invention provides the new bacterial strain of a strain, this strains separation is in the active sludge of wastewater treatment aeration tank, industry park, and separating step is simple, and preparation cost is lower.Described strain growth is rapid, and environmental compatibility is strong, has the dyestuff degradation capability of wide spectrum, and multiple dyestuff is all had to good decolorizing effect, cultivates 10h and can reach maximum percent of decolourization, and percent of decolourization can reach more than 90%, and maximum decolouring concentration reaches 5000mg/L.
Described bacterial strain is applied to dyeing waste water in the present invention and relevant decoloring dye waste water is processed, can solve and in prior art, lack the features such as efficient degradation effect, adaptive capacity to environment be poor, can effectively avoid the dye molecule that is difficult to natural degradation directly to enter the environmental pollution that water body causes, and non-secondary pollution, has good ecological efficiency and application prospect.
The present invention first by Methionin genus bacillus ( lysinibacillus sp.) be applied to dyestuff degraded, filled up domestic technique blank, for solution dye wastewater, process especially decolouring problem strong technical foundation is provided.
Accompanying drawing explanation
Fig. 1: lysinibacillus sp.the scanning electron microscope (SEM) photograph of FS1, scale=1 μ m.
Fig. 2: lysinibacillus sp.the phylogenetic tree of FS1.
Fig. 3: lysinibacillus sp.the decolorizing effect of FS1 to different concns methylene blue.
Fig. 4: lysinibacillus sp.the decolorizing effect of FS1 to different concns toluylene red.
Fig. 5: lysinibacillus sp.the decolorizing effect of FS1 to different concns tropeolin-D.
Fig. 6: lysinibacillus sp.the decolorizing effect of FS1 to different concns Acid Red B.
Fig. 7: lysinibacillussp. FS1 is to the absorption of Acid Red B and degraded.
Fig. 8: lysinibacillussp. the spectral scan figure of FS1 degraded Acid Red B.
Embodiment
Below in conjunction with the drawings and specific embodiments, further illustrate the present invention.Unless stated otherwise, the principle that the present invention adopts and method, equipment are conventional principle, the method and apparatus using in this area.
embodiment 1 lysinibacillus sp. fS1the separation of bacterial strain obtains
1. bacterial strain activation and enrichment
Get 50mL active sludge sample (taking from wastewater treatment aeration tank, industry park, Guangzhou, Guangdong), put into the Erlenmeyer flask (having several granulated glass spherees) that fills 50mL sterilized water, in shaking table vibration, 30min fully breaks up mud sample.Get the suspension mud sample 5mL after breaing up, be seeded in the enrichment medium that fills 100mL, add the Acid Red B dyestuff of 30mg/L, 37 ℃, 200rpm shaking culture 16～24h.
Consisting of of described enrichment medium: extractum carnis 1g, peptone 2g, glucose 3g, K 2hPO 42g, NaH 2pO 40.5g, MgSO 47H 2o 0.2g, MnSO 47H 2o 0.02g, FeCl 37H 2o 0.01g, CaCl 20.02g, sterilized water 1L, pH 7.2; By substratum at 121 ℃ of sterilizing 15～30min and get final product.
2. bacterial strain screening and purifying
By the nutrient solution having after the enrichment of obvious decolorizing effect, with stroke-physiological saline solution test tube stepwise dilution to 10 -1, 10 -2, 10 -3, 10 -4, 10 -5, 10 -6, 10 -7totally 7 gradients.Each gradient is got the diluent of 0.1mL and is coated on the screening culture medium flat board of the Acid Red B that contains 30mg/L, cultivates 24～48h for 37 ℃.Whether observe single bacterial strain has decolouring to iris out now around.Picking has the bacterial strain of decoloring ability, 37 ℃ of continuous streak inoculations to screening culture medium flat board, be further purified and confirm to obtain a high-efficiency degradation dyestuff Methionin Bacillus strain ( lysinibacillus sp.) fS1, described bacterial strain is bacillus, Gram-positive, bacterium colony is circular, smooth surface is flat, neat in edge, faint yellow, opaque, have pearliness, a colony diameter 2mm～6mm, the electron-microscope scanning figure that accompanying drawing 1 is bacterial strain.The sequence of this bacterial strain 16S rDNA with lysinibacillus fusiformisnBRC 15717(NCBI accession number AB271743) there is 99% homology.The phylogenetic tree of this bacterial strain is shown in accompanying drawing 2.
Described bacterial strain is preserved in to Chinese Typical Representative culture collection center (address is Hubei China province Wuhan City Luo Jia Shan Wuhan University) on November 10th, 2013, and deposit number is: CCTCC NO:M 2013561.Be preserved in Chinese Typical Representative culture collection center, deposit number is CCTCC NO:M 2013561.
embodiment 2 lysinibacillus sp.the decolorizing effect test of FS1 to different concns methylene blue
The bacterial strain FS1 of efficient degradation dyestuff of the present invention is inoculated in liquid nutrient medium, and under 30～37 ℃, 150rpm, shaking table is cultivated the nutrient solution that 10～24h obtains described bacterial strain; Consisting of of described liquid nutrient medium: glucose 2g, ammonium chloride 1g, NaH 2pO 40.5g, K 2hPO 42g, FeSO 47H 2o 0.01g, MgSO 47H 2o 0.2g, CaCl 20.02g, water 1L, pH7.2.
(OD takes the logarithm vegetative period 600=1) lysinibacillus sp.fS1 nutrient solution, by 1%(V/V) access in the decolouring basic medium of 100mL, add respectively the methylene blue dye of different concns gradient (80mg/L, 500mg/L, 2000mg/L, 3000mg/L, 4000mg/L, 5000mg/L).Three parallel, and normal temperature is cultivated, and measures the light absorption value of nutrient solution every 5～24 h.
With percent of decolourization, investigate the decoloring ability of bacterial strain FS1.At 200～800(nm) light absorption value of wavelength region interscan dye solution, select the wavelength of maximum absorption band M as the mensuration wavelength of dyestuff light absorption value.By nutrient solution to be measured centrifugal 10min under 5000rpm condition.Get supernatant liquid and measure light absorption value.According to formula, calculate percent of decolourization:
A 0: the light absorption value that nutrient solution is initial; A 1: cultivate the light absorption value after for some time
As shown in Figure 3, when methylene blue concentration is 80～500mg/L, percent of decolourization just can reach more than 80% result in 10h.Although when methylene blue concentration is during higher than 500mg/L, bacterial strain FS1 along with dye strength raises and reduces, still has certain decoloring ability to its percent of decolourization under 4000mg/L concentration, percent of decolourization is 40%.Bacterial strain lysinibacillus sp.fS1 to the highest decolouring concentration of methylene blue up to 4000mg/L.
embodiment 3 lysinibacillus sp.the decolorizing effect test of FS1 to different concns toluylene red
With reference to embodiment 2, cultivate described bacterial strain, (OD takes the logarithm vegetative period 600=1) bacterial strain FS1 nutrient solution, by 1%(V/V) access in the decolouring basic medium of 100mL, add respectively the toluylene red dyestuff of different concns gradient (80mg/L, 300mg/L, 500mg/L, 1000mg/L, 2000mg/L).Other are with embodiment 2.
As shown in Figure 4, when toluylene red concentration is 300～1000mg/L, percent of decolourization reaches maximum percent of decolourization 90% left and right at 10h to test-results substantially, and when concentration raises, the growth of bacterial strain bleaching time is about 48h.Along with the raising of toluylene red concentration, bacterial strain declines gradually to its decoloring ability, by 99%, drops to 30%.Bacterial strain lysinibacillus sp. FS1the red the highest decolouring concentration of centering is up to 2000mg/L.
embodiment 4 lysinibacillus sp.the decolorizing effect test of FS1 to different concns tropeolin-D
With reference to embodiment 2, cultivate described bacterial strain, (OD takes the logarithm vegetative period 600=1) bacterial strain FS1 nutrient solution, by 1%(V/V) access in the decolouring basic medium of 100mL, add respectively the methyl orange dye of different concns gradient (80mg/L, 200mg/L, 300mg/L, 400mg/L, 500mg/L).Other are with embodiment 2.
As shown in Figure 5, when tropeolin-D concentration is 80～300mg/L, 10h percent of decolourization is 90% left and right to result.But when further raising, concentration when (400～500mg/L), although percent of decolourization declines, also can remove 60% left and right.Bacterial strain lysinibacillus sp. FS1the best decolouring concentration to tropeolin-D is 500mg/L.
embodiment 5 lysinibacillus sp. FS1decolorizing effect checking to different concns Acid Red B
(OD takes the logarithm vegetative period 600=1) bacterial strain FS1 nutrient solution, press 1%(V/V) access in the decolouring basic medium of 100mL, add respectively the Acid Red B dyestuff of different concns gradient (80mg/L, 300mg/L, 500mg/L, 1000mg/L, 2000mg/L, 3000mg/L, 4000mg/L, 5000mg/L).Other are with embodiment 2.
As shown in Figure 6, when Acid Red B concentration is 80～1000mg/L, percent of decolourization reaches maximum 90% left and right at 10～24h to result substantially.Although along with Acid Red B concentration is brought up to 5000mg/L by 80mg/L, bacterial strain declines by 93% gradually to its decoloring ability, minimum also have 28%.There is obvious degradation and decolorization treatment effect.Bacterial strain FS1 to the highest decolouring concentration of Acid Red B up to 5000mg/L.
embodiment 6 lysinibacillussp. FS1 to Acid Red B absorption the confirmatory experiment with degradation effect
(OD takes the logarithm vegetative period 600nm=1) bacterial strain FS1 nutrient solution, wherein one group is carried out autoclaving as deactivation group, and another organizes not deactivation as not deactivation group.Two groups of bacterium liquid are fixed with sodium alginate respectively, make the immobilized spherule of diameter 5mm left and right, by 1% inoculum size, are inoculated in the decolouring substratum that contains 80mg/L Acid Red B.Other are with embodiment 2.
As shown in Figure 7, not deactivation group percent of decolourization when 20h just can reach 93% left and right to result, and deactivation group percent of decolourization maintains in 0～7% always.The percent of decolourization of deactivation group declines because thalline and bead absorption cause, and the percent of decolourization of not deactivation group to decline be that absorption and degradation by bacteria acting in conjunction cause.
In addition, in culturing process every 3h the light absorption value at not deactivation group of 200～800nm wavelength region interscan nutrient solution spectrum.As shown in Figure 8, the curve of spectrum is followed successively by the spectral scan curve of 0h, 3h, 6h, 9h, 12h, 15h, 18h to result from top to bottom.Visible, when 0h, the light absorption value of maximum absorption band 515nm is 2.2, while cultivating to 3h, 6h, 9h, 12h, 15h, 18h, the light absorption value of maximum absorption band is down to respectively 2.0,1.9,1.7,1.5,0.3, and percent of decolourization is respectively 10%, 14%, 23%, 32%, 86%; From figure, can find the growth along with incubation time, the light absorption value of maximum absorption band declines gradually, there is no maximum absorption band when 18h, and curve is substantially level and smooth, shows Acid Red B quilt really lysinibacillussp. FS1 degrades.Therefore, of the present invention lysinibacillussp. FS1 is mainly to realize by the biological degradation of bacterial strain to the decolorization of dyestuff.
SEQUENCE LISTING
<110> Ji'nan University
The bacterial strain of <120> mono-high-efficiency degradation dyestuff
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1413
<212> DNA
The sequence of <213> bacterial strain 16S rDNA
<400> 1
ggctggctcc aaaaggttac ctcaccgact tcgggtgtta caaactctcg tggtgtgacg 60
ggcggtgtgt acaaggcccg ggaacgtatt caccgcggca tgctgatccg cgattactag 120
cgattccggc ttcatgtagg cgagttgcag cctacaatcc gaactgagaa cgactttatc 180
ggattagctc cctctcgcga gttggcaacc gtttgtatcg tccattgtag cacgtgtgta 240
gcccaggtca taaggggcat gatgatttga cgtcatcccc accttcctcc ggtttgtcac 300
cggcagtcac cttagagtgc ccaactaaat gatggcaact aagatcaagg gttgcgctcg 360
ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc accacctgtc 420
accgttgccc ccgaagggga aaccatatct ctacagtggt caacgggatg tcaagacctg 480
gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt gcgggccccc 540
gtcaattcct ttgagtttca gtcttgcgac cgtactcccc aggcggagtg cttaatgcgt 600
tagctgcagc actaaggggc ggaaaccccc taacacttag cactcatcgt ttacggcgtg 660
gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgcgcctcag tgtcagttac 720
agaccagata gtcgccttcg ccactggtgt tcctccaaat ctctacgcat ttcaccgcta 780
cacttggaat tccactatcc tcttctgcac tcaagtctcc cagtttccaa tgaccctcca 840
cggttgagcc gtgggctttc acatcagact taagaaacca cctgcgcgcg ctttacgccc 900
aataattccg gacaacgctt gccacctacg tattaccgcg gctgctggca cgtagttagc 960
cgtggctttc taataaggta ccgtcaaggt acagccagtt actactgtac ttgttcttcc 1020
cttacaacag agttttacga accgaaatcc ttcttcactc acgcggcgtt gctccatcag 1080
gctttcgccc attgtggaag attccctact gctgcctccc gtaggagtct gggccgtgtc 1140
tcagtcccag tgtggccgat caccctctca ggtcggctac gcatcgtcgc cttggtgagc 1200
cgttacctca ccaactagct aatgcgccgc gggcccatcc tatagcgaca gccgaaaccg 1260
tctttcaata tttcaccatg aggtgaaaca gattattcgg tattagcccc ggtttcccgg 1320
agttatccca aactataagg taggttgccc acgtgttact cacccgtccg ccgctaacgt 1380
caaaggagca agctccttct ctgttcgctc gac 1413

Claims (7)

  1. The bacterial strain of one high-efficiency degradation dyestuff ( lysinibacillus sp.) FS1, being preserved in Chinese Typical Representative culture collection center, deposit number is CCTCC NO:M 2013561.
  2. 2. according to the bacterial strain FS1 of efficient degradation dyestuff described in claim, it is characterized in that, the sequence of described bacterial strain 16S rDNA is as shown in SEQ ID NO.1.
  3. 3. according to the bacterial strain FS1 of efficient degradation dyestuff described in claim, it is characterized in that, described bacterial strain is bacillus, Gram-positive, bacterium colony is circular, smooth surface is flat, neat in edge, faint yellow, opaque, have pearliness, a colony diameter 2mm～6mm.
  4. 4. the separation method of the bacterial strain FS1 of efficient degradation dyestuff described in claim 1,2 or 3, it is characterized in that, be by the active sludge sample of collection through bacterial classification enrichment medium enrichment culture to having after obvious decolouring phenomenon, gradient dilution, bacterium liquid after gradient dilution is applied on screening culture medium flat board, cultivate 24～48h, the picking circle that obviously decolours, ruling continuously, choose single bacterium colony for 37 ℃ cultivates until after without miscellaneous bacteria in screening culture medium, picking list bacterium colony is in decolouring substratum, in 37 ℃ of standing cultivations and get final product.
  5. 5. separation method according to claim 4, is characterized in that, the consisting of of described enrichment medium: extractum carnis 1g, peptone 2g, glucose 3g, K 2hPO 42g, NaH 2pO 40.5g, MgSO 47H2O 0.2g, MnSO 47H2O 0.02g, FeCl 37H 2o 0.01g, CaCl 20.02g, sterilized water 1L, pH 7.2;
    Consisting of of described screening culture medium: yeast powder 5g, sodium-chlor 5g, peptone 10g, agar 20g, sterilized water 1L, pH7.2;
    Consisting of of described decolouring substratum: carbon source 2g, nitrogenous source 1g, NaH 2pO 40.5g, K 2hPO 42g, FeSO 47H 2o 0.01g, MgSO 47H 2o 0.2g, CaCl 20.02g, sterilized water 1L, pH 7.2.
  6. 6. the cultural method of the bacterial strain FS1 of efficient degradation dyestuff described in claim 1,2 or 3, is characterized in that, described bacterial classification is inoculated in liquid nutrient medium, and at 30～37 ℃, shaking table is cultivated the nutrient solution that 10～24h obtains described bacterial strain.
  7. 7. the cultural method of the bacterial strain FS1 of efficient degradation dyestuff according to claim 6, is characterized in that, the consisting of of described liquid nutrient medium: glucose 2g, ammonium chloride 1g, NaH 2pO 40.5g, K 2hPO 42g, FeSO 47H 2o 0.01g, MgSO 47H 2o 0.2g, CaCl 20.02g, water 1L, pH7.2.
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* Cited by examiner, † Cited by third party
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CN108118013A (en) * 2017-03-15 2018-06-05 广西大学 One plant of bacterial strain and its application for being used to purify molasses alcohol waste water
CN108503041A (en) * 2018-03-20 2018-09-07 许昌学院 A method of handling yuba wastewater using microorganism battery
CN114437979A (en) * 2022-02-14 2022-05-06 恒臻(无锡)生物科技有限公司 Arthrobacter capable of degrading erucamide, and acquisition method, culture method and application thereof
CN114437979B (en) * 2022-02-14 2023-10-24 恒臻(无锡)生物科技有限公司 Arthrobacter capable of degrading erucamide, and acquisition method, culture method and application thereof

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