CN102584421B - Cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet and preparation and using method thereof - Google Patents
Cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet and preparation and using method thereof Download PDFInfo
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Abstract
The invention provides a cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet and a preparation and using method thereof. The cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is a nutrient prepared by a formula and a method which are scientifically and reasonably designed according to the growing conditions of cured material bag-cultured ganoderma lucidum. According to the ganoderma lucidum energy-concentrating nutritional bullet, the yield of the ganoderma lucidum satisfying commodity characters is greatly improved, the biological efficiency can be improved by over 50 percent, and the production period is short, so the ganoderma lucidum energy-concentrating nutritional bullet has very good popularization value. The ganoderma lucidum energy-concentrating nutritional bullet and the method are applied to the production of the cured material bag-cultured ganoderma lucidum.
Description
Technical field:
The invention provides cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet and preparation and application thereof, belong to fungus growing technique field.
Background technology:
Glossy ganoderma is the class of food (medicine) with bacterium, belongs to wooden saprophytic fungus, the fungi-medicine that Ye Shi China is traditional, and as traditional Chinese medicine and the existing history in thousand of invigorant of promoting longevity, domestic market potential is huge, and development glossy ganoderma production industry has a extensive future.The method of current cultivating ganoderma has segment wood cultivated, the method such as substituting stuff cultivation, soil covering culture, but does not limit by place, so substituting stuff cultivation obtains spread due to substituting stuff cultivation.Substituting stuff cultivation mainly contains cultivation in raw material, grog cultivation and material cultivation three kinds of forms of becoming thoroughly decomposed.Wherein, grog cultivation loads in plastics bag by planting material, after then carrying out high-temperature sterilization, carries out a kind of method inoculated, produce after cooling, because planting material is after high-temperature sterilization, cooked, so be called grog cultivation.Grog cultivation is compared with other planting forms, and have fruiting fast, with short production cycle, bacterial contamination rate is low, and production process is easy to the advantages such as management, and becomes the main ganoderma lucidum cultivation method promoted.
Glossy ganoderma, commercially price is also considerable, and Ganoderma spore powder is also just more considerable.But because glossy ganoderma needs a certain proportion of carbon source, nitrogenous source, temperature, humidity, oxygen and gas concentration lwevel etc. in the course of cultivation, and the growth of glossy ganoderma these factors required change according to growth cycle.Therefore, a lot of plantation family is difficult to the output increased cured material bag-cultured ganoderma lucidum, and height is large, and shape is good-looking, especially the second damp glossy ganoderma, and head is little, is difficult to reach commodity requirement.
The invention provides cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet and preparation and application thereof.It is a kind of nutrient substance improving glossy ganoderma output and commodity property be prepared into according to the scientific and reasonable component design of the growth conditions of cured material bag-cultured ganoderma lucidum and method.Ganoderma lucidum energy-concentrating nutritional bullet prepared by the application of the invention, the glossy ganoderma output meeting commodity property improves greatly, and biologicak efficiency can improve more than 50%, with short production cycle, and therefore the present invention has good promotional value.The present invention is generally applicable to the production of cured material bag-cultured ganoderma lucidum.
Summary of the invention:
Cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet and preparation and application thereof are the object of this invention is to provide.The moiety of ganoderma lucidum energy-concentrating nutritional bullet has agar, VITMAIN B1, Lin Suanna Vitamin B2 Sodium Phosphate, vitamins C, magnesium sulfate (MgSO2), zinc sulfate (ZnSO2), potassium primary phosphate (KH2PO4), sodium bicarbonate (NaHCO3), spearmint oil.Preparation method is the mass percent according to each composition in formula, first with a certain amount of water heating and melting agar, after melting, add the VITMAIN B1 in formula, Lin Suanna Vitamin B2 Sodium Phosphate, magnesium sulfate (MgSO2), zinc sulfate (ZnSO2), potassium primary phosphate (KH2PO4), after being cooled to certain temperature after mixing, add the vitamins C in formula again, sodium bicarbonate (NaHCO3), spearmint oil, after being cooled to certain temperature again after stirring, be reprocessed into the cylindric of a certain size, be namely prepared into cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet.Using method is after planting material is installed bag, then after using round log bastinade hole to certain depth, after extracting round log rod, more a certain amount of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is put into hole, finally turn back in hole by round log rod again, fasten sack.
Cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet moiety provided by the present invention has: agar, vitamins B
1, vitamins B
2, vitamins C, magnesium sulfate (MgSO
2), zinc sulfate (ZnSO
2), potassium primary phosphate (KH
2pO4), sodium bicarbonate (NaHCO
3), spearmint oil.Wherein, described cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet formula is made up of the component of following mass percent: agar (98 ~ 99%), vitamins B
1(0.1 ~ 0.2%), vitamins B
2(0.1 ~ 0.2%), vitamins C (0 ~ 0.5%), magnesium sulfate (MgSO
2) (0.05 ~ 0.1%), zinc sulfate (ZnSO
2) (0.05 ~ 0.1%), potassium primary phosphate (KH
2pO4) (0.1 ~ 0.4%), sodium bicarbonate (NaHCO
3) (0.5 ~ 1%), spearmint oil (0.1 ~ 0.5%).
The preparation method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet of the present invention, comprises the following steps:
1) first with a certain amount of water heating and melting agar.Wherein 1g agar amount of water is 5 ~ 15ml; Heating temperature is 70 ~ 90 DEG C.
2) by step 1) in melt after agar, add the vitamins B in described formula
1, vitamins B
2, vitamins C, magnesium sulfate (MgSO
2), zinc sulfate (ZnSO
2), potassium primary phosphate (KH
2pO4), mix.
3) by step 2) in the solution that obtains be cooled to 45 ~ 55 DEG C, then add the vitamins C in described formula, sodium bicarbonate (NaHCO
3), spearmint oil, be cooled to solution after stirring again and solidify.
4) by step 3) in the coagulum that obtains, be reprocessed into high 1 ~ 3cm, diameter is the cylindric of 0.4 ~ 1cm.
By above-mentioned steps, be namely prepared into cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet.
The using method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet of the present invention is:
After planting material is installed bag, then with diameter be 1.5 ~ 2cm round log bastinade pitch-row pocket at the bottom of 2 ~ 5cm, after extracting round log rod, more a certain amount of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is put into hole, finally again round log rod is turned back in hole, fasten sack;
Usage quantity is: be less than or equal to 1kg planting material/bag, and every bag uses 1 cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet; Be greater than 1kg planting material/bag, every bag uses 2 cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullets.
Specific embodiment:
Below by specific embodiment, working method of the present invention is described, but the present invention does not limit to therewith.Method described in following embodiment, if no special instructions, is ordinary method; Described reagent and material and facility, if no special instructions, all can obtain from commercial channels.
Embodiment 1.
Cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet formula is made up of the component of following mass percent: agar (98%), vitamins B
1(0.2%), vitamins B
2(0.1%), vitamins C (0.5%), magnesium sulfate (MgSO
2) (0.05%), zinc sulfate (ZnSO
2) (0.05%), potassium primary phosphate (KH
2pO4) (0.1%), sodium bicarbonate (NaHCO
3) (0.5%), spearmint oil (0.5%).
The preparation method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet, comprises the following steps:
1) first with the agar in formula described in a certain amount of water heating and melting.Wherein 1g agar amount of water is 15ml; Heating temperature is 90 DEG C.
2) by step 1) in melt after agar, add the vitamins B in described formula
1, vitamins B
2, vitamins C, magnesium sulfate (MgSO
2), zinc sulfate (ZnSO
2), potassium primary phosphate (KH
2pO4), mix.
3) by step 2) in the solution that obtains be cooled to 55 DEG C, then add the vitamins C in described formula, sodium bicarbonate (NaHCO
3), spearmint oil, be cooled to solution after stirring again and solidify.
4) by step 3) in the coagulum that obtains, be reprocessed into high 3cm, diameter is the cylindric of 0.6cm.
By above-mentioned steps, be namely prepared into cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet.
The using method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is:
After planting material is installed bag, then with diameter be 1.5cm round log bastinade pitch-row pocket at the bottom of 2cm, after extracting round log rod, more a certain amount of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is put into hole, finally again round log rod is turned back in hole, fasten sack;
Usage quantity is: 1kg planting material/bag, and every bag uses 1 cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet.
Under the management of same cultivation condition and planting material, use the cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet prepared of the present invention comparatively traditional method first damp Ganoderma sporophore shorten 4 days vegetative period, and second damp fresh sesame also can reach commodity requirement, biologicak efficiency improves greatly, is compared as follows table 1 by cultivating ganoderma of the present invention and traditional method cultivating ganoderma:
The comparison of table 1. different methods cultivating ganoderma
Note: in table, the first damp sporophore growth number of days refers to from occurring that mushroom flower bud reaches the number of days required for commodity requirement for the first time.Two damp sporophore growth number of days reach the number of days required for commodity requirement after referring to and having gathered in the first tide; The first tide and the total amount (kg) of the second damp fresh sesame and percent value of planting material weight that total biologicak efficiency refers to.
Embodiment 2.
Cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet formula is made up of the component of following mass percent: agar (99%), vitamins B
1(0.1%), vitamins B
2(0.1%), vitamins C (0%), magnesium sulfate (MgSO
2) (0.05%), zinc sulfate (ZnSO
2) (0.05%), potassium primary phosphate (KH
2pO4) (0.1%), sodium bicarbonate (NaHCO
3) (0.5%), spearmint oil (0.1%).
The preparation method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet, comprises the following steps:
1) first with a certain amount of water heating and melting agar.Wherein 1g agar amount of water is 10ml; Heating temperature is 80 DEG C.
2) by step 1) in melt after agar, add the vitamins B in described formula
1, vitamins B
2, vitamins C, magnesium sulfate (MgSO
2), zinc sulfate (ZnSO
2), potassium primary phosphate (KH
2pO4), mix.
3) by step 2) in the solution that obtains be cooled to 55 DEG C, then add the vitamins C in described formula, sodium bicarbonate (NaHCO
3), spearmint oil, be cooled to solution after stirring again and solidify.
4) by step 3) in the coagulum that obtains, be reprocessed into high 2cm, diameter is the cylindric of 0.8cm.
By above-mentioned steps, be namely prepared into cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet.
The using method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is:
After planting material is installed bag, then with diameter be 2cm round log bastinade pitch-row pocket at the bottom of 2cm, after extracting round log rod, more a certain amount of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is put into hole, finally again round log rod is turned back in hole, fasten sack;
Usage quantity is: 2kg planting material/bag, and every bag uses 2 cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullets.
Under the management of same cultivation condition and planting material, use the cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet prepared of the present invention comparatively traditional method first damp Ganoderma sporophore shorten 7 days vegetative period, and second damp fresh sesame also can reach commodity requirement, biologicak efficiency improves greatly, is compared as follows table 2 by cultivating ganoderma of the present invention and traditional method cultivating ganoderma:
The comparison of table 2. different methods cultivating ganoderma
Note: in table, the first damp sporophore growth number of days refers to from occurring that mushroom flower bud reaches the number of days required for commodity requirement for the first time.Two damp sporophore growth number of days reach the number of days required for commodity requirement after referring to and having gathered in the first tide; The first tide and the total amount (kg) of the second damp fresh sesame and percent value of planting material weight that total biologicak efficiency refers to.
Embodiment 3.
Cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet formula is made up of the component of following mass percent: agar (98.5%), vitamins B
1(0.1%), vitamins B
2(0.1%), vitamins C (0.2%), magnesium sulfate (MgSO
2) (0.1%), zinc sulfate (ZnSO
2) (0.1%), potassium primary phosphate (KH
2pO4) (0.25%), sodium bicarbonate (NaHCO
3) (0.5%), spearmint oil (0.15%).The preparation method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet, comprises the following steps:
5) first with a certain amount of water heating and melting agar.Wherein 1g agar amount of water is 6ml; Heating temperature is 75 DEG C.
6) by step 1) in melt after agar, add the vitamins B in described formula
1, vitamins B
2, vitamins C, magnesium sulfate (MgSO
2), zinc sulfate (ZnSO
2), potassium primary phosphate (KH
2pO4), mix.
7) by step 2) in the solution that obtains be cooled to 48 DEG C, then add the vitamins C in described formula, sodium bicarbonate (NaHCO
3), spearmint oil, be cooled to solution after stirring again and solidify.
8) by step 3) in the coagulum that obtains, be reprocessed into high 1.5cm, diameter is the cylindric of 1cm.
By above-mentioned steps, be namely prepared into cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet.
The using method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is:
After planting material is installed bag, then with diameter be 1.5cm round log bastinade pitch-row pocket at the bottom of 2cm, after extracting round log rod, more a certain amount of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is put into hole, finally again round log rod is turned back in hole, fasten sack;
Usage quantity is: 1kg planting material/bag, and every bag uses 1 cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet.
Same cultivation bar be part management and planting material under, use the cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet prepared of the present invention comparatively traditional method first damp Ganoderma sporophore shorten 6 days vegetative period, and second damp fresh sesame also can reach commodity requirement, biologicak efficiency improves greatly, is compared as follows table 3 by cultivating ganoderma of the present invention and traditional method cultivating ganoderma:
The comparison of table 3. different methods cultivating ganoderma
Note: in table, the first damp sporophore growth number of days refers to from occurring that mushroom flower bud reaches the number of days required for commodity requirement for the first time.Two damp sporophore growth number of days reach the number of days required for commodity requirement after referring to and having gathered in the first tide; The first tide and the total amount (kg) of the second damp fresh sesame and percent value of planting material weight that total biologicak efficiency refers to.
Claims (2)
1. cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet, is characterized in that described cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet is made up of the component of following mass percent: agar 98 ~ 99%, vitamins B
10.1 ~ 0.2%, vitamins B
20.1 ~ 0.2%, vitamins C 0 ~ 0.5%, magnesium sulfate 0.05 ~ 0.1%, zinc sulfate 0.05 ~ 0.1%, potassium primary phosphate 0.1 ~ 0.4%, sodium bicarbonate 0.5 ~ 1%, spearmint oil 0.1 ~ 0.5%.
2. the preparation method of cured material bag-cultured ganoderma lucidum energy-concentrating nutritional bullet as claimed in claim 1, comprises the following steps:
1) with a certain amount of water heating and melting agar, wherein 1g agar amount of water is 5 ~ 15ml; Heating temperature is 70 ~ 90 DEG C;
2) in step 1) melt after agar in, add vitamins B
1, vitamins B
2, magnesium sulfate, zinc sulfate, potassium primary phosphate, mixes;
3) by step 2) in the solution that obtains be cooled to 45 ~ 55 DEG C, then add vitamins C, sodium bicarbonate, spearmint oil, be cooled to solution after stirring again and solidify;
4) by step 3) in the coagulum that obtains, be processed into high 1 ~ 3cm, diameter is the cylindric of 0.4 ~ 1cm.
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1049263A (en) * | 1989-08-05 | 1991-02-20 | 谢宏年 | Mushrooms growing agent |
CN1050484A (en) * | 1989-09-29 | 1991-04-10 | 赵清华 | The preparation of edible fungus production increasing agent and using method |
CN1093224A (en) * | 1993-04-09 | 1994-10-12 | 南通市蔬菜科学研究所 | Edible mushroom special sterilizing volume increase agent |
CN1123837A (en) * | 1995-05-26 | 1996-06-05 | 刘德科 | Cultivation of fungus |
CN101172902A (en) * | 2007-10-23 | 2008-05-07 | 贵州大学 | Method for formulating culture medium additive agent for cultivation of selenium-rich ganoderma lucidum |
CN101391922A (en) * | 2008-11-10 | 2009-03-25 | 马宏达 | Formulating method of yield nutrient fluid for inonotus obliquus cultivation |
-
2011
- 2011-12-27 CN CN201110443486.2A patent/CN102584421B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1049263A (en) * | 1989-08-05 | 1991-02-20 | 谢宏年 | Mushrooms growing agent |
CN1050484A (en) * | 1989-09-29 | 1991-04-10 | 赵清华 | The preparation of edible fungus production increasing agent and using method |
CN1093224A (en) * | 1993-04-09 | 1994-10-12 | 南通市蔬菜科学研究所 | Edible mushroom special sterilizing volume increase agent |
CN1123837A (en) * | 1995-05-26 | 1996-06-05 | 刘德科 | Cultivation of fungus |
CN101172902A (en) * | 2007-10-23 | 2008-05-07 | 贵州大学 | Method for formulating culture medium additive agent for cultivation of selenium-rich ganoderma lucidum |
CN101391922A (en) * | 2008-11-10 | 2009-03-25 | 马宏达 | Formulating method of yield nutrient fluid for inonotus obliquus cultivation |
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Effective date of registration: 20210111 Address after: No.26, Baidi Road, Nankai District, Tianjin 300192 Patentee after: Tianjin Academy of Agricultural Sciences Address before: 300192 5-1-901 Hangyu apartment, Baidi Road, Nankai District, Tianjin Patentee before: TIANJIN FORESTRY INSTITUTE OF FRUIT TREES |