CN102577949A - Intermediate propagation method for tea germchit - Google Patents

Abstract

The invention discloses an intermediate propagation method for tea germchit. First, new tips of a tea tree are cut into stem sections, and then the stem sections are disinfected. The disinfected stem sections are inserted into a medium and radiation-cultured under appropriate light so as to enable lateral buds to grow new tips, and the steps are repeated for propagation. The intermediate propagation method for the tea germchit is simple in technology, convenient to operate, fast in propagation speed and low in cost. Propagation multiple within 25 to 35 days is increased by 4 to 5 times, and one bud can propagate 1.2 million germchit in one year.

Description

A kind of method for quickly breeding of tealeaves seedling

Technical field

The present invention relates to the plant seedling breeding, more specifically relate to a kind of method for quickly breeding of tealeaves seedling.

Background technology

Tealeaves belongs to Theaceae Camellia plant, comes from China, existing more than 3000 year of the history of among the people kind of tea of China, tea making.Mainly to produce genetic resources, cultivation step, the tea making technology of tea country and the custom of drinking tea etc. all be to pass directly or indirectly from China in the world now.

Cuttage and two kinds of methods of planting seed are generally adopted in the breeding of tea tree.Cottage propagation is the main method of tealeaves breeding, is exactly clip tea tree branch, under certain conditions, is inserted in soil, sand or other matrix, makes it take root, come into leaves, and becomes the new plant of complete and independent.But this method, required vegetable material is a lot, the maternal plant material seldom the time its reproduction speed too slow.Use seminal propagation, the offspring separates, and can not keep maternal good characteristic.

Plant tissue culture technique belongs to biological technical field, and it is a totipotency of utilizing plant cell, and the little block organization of cultured in vitro plant carries out the industrial breeding technique of plant Clonal Rapid Propagation.It only needs plant tissue material seldom, and reproduction speed is fast, does not receive season limit.Can be at the short-term high quality seedling under maternal plant situation seldom with this method.

Summary of the invention

The objective of the invention is, a kind of method for quickly breeding of tealeaves seedling is provided, this method is simple, easy to operate, reproduction speed is fast, can accelerate the quick breeding of tealeaves new varieties fine individual plant and the update of local tea variety.

In order to achieve the above object, the present invention adopts following technical scheme:

A kind of method for quickly breeding of tealeaves seedling specifically may further comprise the steps:

(1), with the tea tree young sprout as explant, young sprout is cut into 2-3 centimetre of stem section;

(2), with the stem section of downcutting, at first in the ethanolic solution of 70-75%, soaked 2-3 minute, and then with 0.1% HgCl ₂Solution soaking 8-12 minute, during do not stop shake, clean with aseptic water washing then;

(3), sterile stem section is seeded in inducing culture on the young sprout inducing culture, wherein,

The constituent that contains in the 1L young sprout inducing culture is:

Agar 6-8g, sucrose 30-32g, NH ₄NO ₃1.5-1.7g, KNO ₃1.85-1.95g, CaCl ₂2H ₂O 0.4-0.45g, MgSO ₄7H ₂O 0.35-0.38g, ZnSO ₄7H ₂O 2-3mg, FeSO ₄7H ₂O 2.5-2.8mg, KH ₂PO ₄0.15-0.18g, KCL50-60mg, Na ₂EDTA2 H ₂O 37.0-37.5mg, inositol 95-105mg, Cobastab ₆0.1-0.2mg, nicotinic acid 0.4-0.6mg, thiamine 0.1-0.15 mg, glycine 1-3mg, zinc sulphate 1-2mg, land plaster 0.3-0.5g, NAA0.01-0.5 mg;

(4), be 20-25 ℃ in temperature, light intensity 1500-3000Lx cultivates under 8-12 hour the condition of illumination every day, makes lateral bud grow young sprout;

(5), every at a distance from 25-35 days, the step that repeats (1), (2), (3), (4) expands numerous.

Technology of the present invention compared with prior art has the following advantages:

Technology is simple, and is easy to operate, and reproduction speed is fast, and cost is low, and 25-35 days breeding rate increase reaches 4-5 doubly, and a bud can be bred 1,200,000 strain seedlings in 1 year.

Embodiment:

A kind of method for quickly breeding of tealeaves seedling as explant, at first is cut into young sprout 2～3 centimetres stem section with the tea tree young sprout, at first in the ethanolic solution of 70-75%, soaked 0.8-1.2 minute, and then with 0.1% HgCl ₂Solution soaking 8-12 minute, during do not stop shake, clean with aseptic water washing then; Then the young sprout that disinfects is inserted in the prepared culture medium, the 3rd is illumination cultivation, is 20～25 ℃ in temperature, light intensity 1600～2500Lx, and illumination every day was cultivated 25-35 days under the condition in 8-12 hour, made lateral bud grow young sprout, repeated above-mentioned steps and expanded numerous.

The constituent that contains in the 1L young sprout inducing culture is:

Agar 6-8g, sucrose 30-32g, NH ₄NO ₃1.5-1.7g, KNO ₃1.85-1.95g, CaCl ₂2H ₂O 0.4-0.45g, MgSO ₄7H ₂O 0.35-0.38g, ZnSO ₄7H ₂O 2-3mg, FeSO ₄7H ₂O 2.5-2.8mg, KH ₂PO ₄0.15-0.18g, KCL50-60mg, Na ₂EDTA2 H ₂O 37.0-37.5mg, inositol 95-105mg, Cobastab ₆0.1-0.2mg, nicotinic acid 0.4-0.6mg, thiamine 0.1-0.15, glycine 1-3mg, zinc sulphate 1-2mg, land plaster 0.3-0.5g, NAA0.01-0.5 mg.

The tea shoot of cultivating through above medium has absorbed multivitamin and trace element, can make these vitamins in seedling, assemble, and makes that the tealeaves that grows has vitamin and micro-gathering after the tea shoot growth.Increase the health care of tealeaves.

Claims (1)

1. the method for quickly breeding of a tealeaves seedling is characterized in that, specifically may further comprise the steps:

(1), the tea tree young sprout is cut into the stem section;

(2), with the stem section of downcutting, at first in the ethanolic solution of 70-75%, soaked 0.8-1.2 minute, and then with 0.1% HgCl ₂Solution soaking 8-12 minute, during do not stop shake, clean with aseptic water washing then;

(3), sterile stem section is seeded in inducing culture on the young sprout inducing culture, wherein:

The constituent ratio that contains in the 1L young sprout inducing culture is:

Agar 6-8g, sucrose 30-32g, NH ₄NO ₃1.5-1.7g, KNO ₃1.85-1.95g, CaCl ₂2H ₂O 0.4-0.45g, MgSO ₄7H ₂O 0.35-0.38g, ZnSO ₄7H ₂O 2-3mg, FeSO ₄7H ₂O 2.5-2.8mg, KH ₂PO ₄0.15-0.18g, KCL50-60mg, Na ₂EDTA2 H ₂O 37.0-37.5mg, inositol 95-105mg, Cobastab ₆0.1-0.2mg, nicotinic acid 0.4-0.6mg, thiamine 0.1-0.15 mg, glycine 1-3mg, zinc sulphate 1-2mg, land plaster 0.3-0.5g, NAA0.01-0.5 mg;

(4), be 20-25 ℃ in temperature, light intensity 1500-3000Lx cultivated 25-35 days under 8-12 hour the condition of illumination every day;

(5), cultivate the tealeaves seedling and forward the earth cultivation to;

(6), the step that repeats (1), (2), (3), (4) expands numerous.