CN102559486A - Full-automatic air microbe sampler - Google Patents
Full-automatic air microbe sampler Download PDFInfo
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- CN102559486A CN102559486A CN2011104362027A CN201110436202A CN102559486A CN 102559486 A CN102559486 A CN 102559486A CN 2011104362027 A CN2011104362027 A CN 2011104362027A CN 201110436202 A CN201110436202 A CN 201110436202A CN 102559486 A CN102559486 A CN 102559486A
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Abstract
The invention discloses a full-automatic air microbe sampler. The sampler comprises a sample collection system and a sample liquid distribution system; wherein the sample collection system comprises a sampling head, a blower, an impact sampling bottle, a liquid storage bottle, an injection pump and a sampling pump; and two outlets of the sampling head are respectively communicated with the blower and the impact sampling bottle; an air inlet of the sampling pump is communicated with the impact sampling bottle; the liquid storage bottle is consequentially communicated with the injection pump and the impact sampling bottle; the sample liquid distribution system comprises a peristaltic pump and an injection device; the peristaltic pump is communicated with the impact sampling bottle, and an outlet of the peristaltic pump is connected with the injection device through a pipeline. According to the full-automatic air microbe sampler, the sampling head can utilize a two-stage virtual impact theory, so that wall damage of particles is reduced, and high particle diameter cutting efficiency is obtained; simultaneously, sampling flow is large, microbial particle collection density is improved, further experimental analysis is facilitated, and sensitivity and accuracy of devices are improved.
Description
Technical field
The present invention relates to a kind of full-automatic air microorganism sampler, particularly a kind of air microorganism sampler that is used for the sampling of biocontainment laboratory ambient air is so that the biological hazard material that check and analysis possibly exist.
Background technology
In constantly the advancing of human society, increasing New Development transmissible disease occurs, and velocity of propagation is more and more faster, is threatening the universe's life and health.The SARS of outburst in 2003 Influenza A H1N1 popular and generation high pathogenic avian influenzas in 2004 and appearance in 2009 has caused threatening with potential of reality for the whole world people of various countries' health, Economic development and even national Biosafety.In order to tackle the New Development transmissible disease; A large amount of high-grade biocontainment laboratories has been set up in countries in the world; Caused also that thus laboratory exhaust gas is to the pollution of environment and to the harm of human health, like FSU's Sverdlovsk city anthrax outbreak of epidemic in 1979; Surrey County, Britain broke out the foot and mouth disease epidemic situation in 2007.The construction quantity of China Higher level biocontainment laboratory after 2005 (comprising BSL-3, ABSL-3, BSL-4 and ABSL-4 laboratory) reaches more than 100; Therefore to air monitoring, sample collection and the processing of laboratory internal and external environment; Become the key of support personnel, environmental organism safety, extremely urgent.
Biological pathogenic material can be propagated through number of ways, and wherein airborne transmission is the most stealthy, the most extensive, hazard area is maximum.Existing abiotic suspended particles also have the biological suspension particle in the air, also have both complex bodys.Harmful organism particle wherein is inhaled into human body, will cause various diseases.Thereby need these harmful microorganism particles separated effectively and collect in the specific liquid, in order to experimental analysis, to control the generation of the communicate illness that biological virulence factor causes effectively.
This needs regularly to gather a large amount of air samples so that biological hazard is in time controlled in experimental analysis.Yet, use more microorganism sampler at present through membrane filtration, the bacterium that collects is less to be unfavorable for detection, or adopts impact sampler, belongs to inertial impaction, causes that easily particle is detained, and does not reach thorough isolating purpose; The moisture that will stick sampling media that air-flow causes too greatly easily dries up, and makes the particle resuspending of having gathered escape to lose with air-flow, and perhaps because of moisture loss causes mikrobe dry dead, and low discharge sampling thief collected specimens causes omission easily.
Summary of the invention
The air microorganism sampler that the purpose of this invention is to provide a kind of full-automatic big flow, it can be automatically in certain intervals repeatedly from air concentrating and separating go out target substance and mix the formation sample liquid with sample solution so that experiment Analysis.
A kind of full-automatic air microorganism sampler provided by the invention comprises sample collecting system and appearance liquid distribution system;
Said sample collecting system comprises sampling head, blower fan, impact sampling bottle, liquid storage bottle, syringe pump and sampling pump; Two outlets of said sampling head are connected with said blower fan and impact sampling bottle respectively; The gas inlet of said sampling pump is connected with said impact sampling bottle; Said liquid storage bottle is connected with said syringe pump and impact sampling bottle successively;
Said appearance liquid distribution system comprises peristaltic pump and injection device; Said peristaltic pump is connected with said impact sampling bottle, and said wriggling pump outlet is connected with said injection device through pipeline.
In the above-mentioned full-automatic air microorganism sampler, the middle and upper part that the place can be located at said impact sampling bottle that is communicated with of said sampling pump and said impact sampling bottle is so that said sampling pump extracts the air in the said impact sampling bottle and forms certain negative pressure.
In the above-mentioned full-automatic air microorganism sampler; The pipeline that said sampling head is connected with said impact sampling bottle extends to the middle and lower part of said impact sampling bottle, so that the target substance in the said sampling head mixes to obtain sample liquid with sample solution after under action of negative pressure, getting into said impact sampling bottle.
In the above-mentioned full-automatic air microorganism sampler, said injection device is the double injection syringe needle, can gather a plurality of samples simultaneously; Said peristaltic pump is connected with said double injection syringe needle through T-valve, and said T-valve is distributed into two-way liquid with sample liquid and transfers to respectively in the said double injection syringe needle.
In the above-mentioned full-automatic air microorganism sampler, said T-valve can be three-way solenoid valve.
In the above-mentioned full-automatic air microorganism sampler, said double injection syringe needle respectively with can make its horizontal drive mechanism that moves in a lateral direction and its mobile in a longitudinal direction vertical drive mechanism is connected.
In the above-mentioned full-automatic air microorganism sampler, said sample collecting system can be connected with flow-control module, temperature control modules and/or time control module; Said appearance liquid distribution system is connected with time control module.
In the above-mentioned air microorganism sampler, said blower fan, sampling pump and syringe pump are connected with said flow-control module, can realize the flow control to air sample and sample solution.
In the above-mentioned full-automatic air microorganism sampler, said blower fan, sampling pump and syringe pump are connected with said time control module, can realize sampling time and isoparametric control of sampling time; Said peristaltic pump is connected with said time control module, can realize appearance liquid is distributed the control of time.
In the above-mentioned full-automatic air microorganism sampler, said impact sampling bottle is connected with said temperature control modules, can control the temperature of the sample liquid in the said impact sampling bottle, when a temperature is lower than zero degree, then starts heating schedule; When its temperature is too high, then start cooling program.
The present invention has the following advantages:
(1) sampling head in the sampling thief provided by the invention can adopt two-stage virtual impact principle, thereby the wall that can reduce particle decreases, obtains higher particle diameter cutting efficiency.Sampling flow is big simultaneously, has improved the mikrobe particle and has gathered concentration, helps the further experiment analysis, improves instrumental sensitivity and accuracy;
(2) the present invention has realized from sample collecting to appearance liquid distribution, the full automatic control of preserving; Collect different time points ambient air sample through presetting sampling time and SI; Realize that through accurate transmission mechanism the sample that different time is collected distributes then; System record sample position and acquisition time, thus realize check and analysis to ambient air in for some time;
(3) the present invention has increased by a sleeving temperature control control modular device, both can realize instrument low temperature (sub-zero zero) work sampling, again can controlled temperature be beneficial to the long-time preservation of sample at lesser temps (above zero).
Description of drawings
Fig. 1 is the structural representation of full-automatic air microorganism sampler provided by the invention.
Each mark is following among the figure: 1 sampling head, 2 blower fans, 3 impact sampling bottles, 4 syringe pumps, 5 liquid storage bottles, 6 sampling pumps, 7 peristaltic pumps, 8 three-way solenoid valves, 9 double injection syringe needles, 10 sample liquid collection tubes.
Embodiment
Below in conjunction with accompanying drawing the present invention is further specified, but the present invention is not limited to following examples.
The full-automatic air microorganism sampler that present embodiment provides comprises sample collecting system and appearance liquid distribution system; This sample collecting system comprises sampling head 1, blower fan 2, impact sampling bottle 3, liquid storage bottle 5, syringe pump 4 and sampling pump 6; Two outlets of sampling head 1 are connected with blower fan 2 and impact sampling bottle 3 respectively, and the pipeline that is connected with impact sampling bottle 3 of sampling head 1 extends to the bottom of impact sampling bottle 3; The gas inlet of sampling pump 6 is connected with the top of impact sampling bottle 3; Pneumatic outlet is free in the air; Can realize that impact sampling bottle 3 bleeds to form negative pressure; Then the sample in the sampling head 1 can feed to impact sampling bottle 3 with corresponding flow, and another part sample in the sampling head 1 then is disposed in the air through the effect of blower fan 2; Liquid storage bottle 5 is connected with syringe pump 4 and impact sampling bottle 3 successively, and the sample solution in the liquid storage bottle 5 can be pumped in the impact sampling bottle 3 under the effect of syringe pump 4, thereby obtains sample liquid with sample mix; Blower fan 2, sampling pump 6 and syringe pump 4 all are connected with the time control module (not shown) with the flow-control module (not shown); Wherein flow-control module is used to realize the accurate control to sample flow and sample liquid flow; Time control module is used for realizing to sampling time and the isoparametric control of time in SI, thereby can collects the different air microbe samples in the different time; Impact sampling bottle 3 is connected with temperature control modules, is used to realize the temperature controlling to sample liquid, when its temperature is lower than too preset temp, can start heating schedule and cooling program respectively; Appearance liquid distribution system comprises peristaltic pump 7 and injection device; Peristaltic pump 7 is connected with impact sampling bottle 3, and the outlet of peristaltic pump 7 is connected with double injection syringe needle 9 through three-way solenoid valve 8; Double injection syringe needle 9 respectively with can make its horizontal drive mechanism (not shown) that moves in a lateral direction and its mobile in a longitudinal direction vertical drive mechanism (not shown) is connected; Laterally drive mechanism can be realized the syringe needle location of double injection syringe needle 9, and vertically drive mechanism can realize that the appearance liquid of double injection syringe needle 9 injects; Peristaltic pump 7 also is connected with the time control module (not shown), can realize appearance liquid is distributed the control of time.
In the above-mentioned full-automatic air microorganism sampler, the type of sampling head 1 and concrete structure can be selected according to actual needs, to realize the collection to the target compound of different-grain diameter; Injection device also can be the many injection needless that are connected with multiport valve.
When using above-mentioned full-automatic air microorganism sampler, at first preestablish the sampling time of each sample and the sampling time interval of sample room, preestablish the sampling quantity of blower fan 2 and sampling pump 6 through flow-control module through time control module; After the sampling beginning, start blower fan 2 and sampling pump 6 simultaneously, the airborne mikrobe particle that sampling head 1 is collected transfers in the impact sampling bottle 3, stops sampling automatically after waiting to arrive setting-up time; Start syringe pump 4 then, the sample solution in the liquid storage bottle 5 is injected into to hit in the sampling bottle 3 under its effect and obtains sample liquid with the mikrobe mix particles that collects; Start peristaltic pump 7 this moment,, sample liquid is injected in the sample liquid collection tube 10 through three-way solenoid valve 8 and double injection syringe needle 9 with sample liquid outlet discharge from impact sampling bottle 3 belows; In the sampling process, the temperature in the impact sampling bottle 3 can be monitored through temperature control modules, if temperature is lower than or too during preset temp, can start heating schedule and cooling program respectively, guarantee that sample liquid is in normal state.
Claims (10)
1. full-automatic air microorganism sampler is characterized in that: said sampling thief comprises a sample collecting system and an appearance liquid distribution system;
Said sample collecting system comprises sampling head, blower fan, impact sampling bottle, liquid storage bottle, syringe pump and sampling pump; Two outlets of said sampling head are connected with said blower fan and impact sampling bottle respectively; The gas inlet of said sampling pump is connected with said impact sampling bottle; Said liquid storage bottle is connected with said syringe pump and impact sampling bottle successively;
Said appearance liquid distribution system comprises peristaltic pump and injection device; Said peristaltic pump is connected with said impact sampling bottle, and said wriggling pump outlet is connected with said injection device through pipeline.
2. sampling thief according to claim 1 is characterized in that: said sampling pump and the place of connection of said impact sampling bottle are located at the middle and upper part of said impact sampling bottle.
3. sampling thief according to claim 1 and 2 is characterized in that: the pipeline that said sampling head is connected with said impact sampling bottle extends to the middle and lower part of said impact sampling bottle.
4. according to arbitrary described sampling thief among the claim 1-3, it is characterized in that: said injection device is the double injection syringe needle; Said peristaltic pump is connected with said double injection syringe needle through T-valve.
5. sampling thief according to claim 4 is characterized in that: said T-valve is a three-way solenoid valve.
6. according to claim 4 or 5 described sampling thiefs, it is characterized in that: said double injection syringe needle respectively with can make its horizontal drive mechanism that moves in a lateral direction and its mobile in a longitudinal direction vertical drive mechanism is connected.
7. according to arbitrary described sampling thief among the claim 1-6, it is characterized in that: said sample collecting system is connected with flow-control module, temperature control modules and/or time control module; Said appearance liquid distribution system is connected with time control module.
8. sampling thief according to claim 7 is characterized in that: said blower fan, sampling pump and syringe pump are connected with said flow-control module.
9. according to claim 7 or 8 described sampling thiefs, it is characterized in that: said blower fan, sampling pump and syringe pump are connected with said time control module; Said peristaltic pump is connected with said time control module.
10. according to arbitrary described sampling thief among the claim 7-9, it is characterized in that: said impact sampling bottle is connected with said temperature control modules.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110436202.7A CN102559486B (en) | 2011-12-22 | 2011-12-22 | Full-automatic air microbe sampler |
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| CN201110436202.7A CN102559486B (en) | 2011-12-22 | 2011-12-22 | Full-automatic air microbe sampler |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106967595A (en) * | 2017-05-16 | 2017-07-21 | 上海德具生物科技有限公司 | Bacterium or viral online acquisition and online automatic detection method in a kind of air |
| CN107118961A (en) * | 2017-05-16 | 2017-09-01 | 上海德具生物科技有限公司 | Bacterium or viral online acquisition and on-line automatic detection device in a kind of air |
| CN107118951A (en) * | 2017-05-16 | 2017-09-01 | 上海德具生物科技有限公司 | One kind sampling integrated sampling box structure |
| CN108426751A (en) * | 2018-01-23 | 2018-08-21 | 宁波市环境监测中心 | A kind of gas sampling assembly and its method |
| CN114279775A (en) * | 2021-12-29 | 2022-04-05 | 军事科学院***工程研究院卫勤保障技术研究所 | Sampling device for monitoring bioaerosol |
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| CN202401061U (en) * | 2011-12-22 | 2012-08-29 | 中国人民解放军军事医学科学院微生物流行病研究所 | Full-automatic air microbe sampler |
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| US20080090289A1 (en) * | 2006-06-06 | 2008-04-17 | Lynntech, Inc. | Microbial Sampling Device |
| CN101643698A (en) * | 2009-07-22 | 2010-02-10 | 福州大学 | Device for sampling aerial influenza viruses and virus collection liquid thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967595A (en) * | 2017-05-16 | 2017-07-21 | 上海德具生物科技有限公司 | Bacterium or viral online acquisition and online automatic detection method in a kind of air |
| CN107118961A (en) * | 2017-05-16 | 2017-09-01 | 上海德具生物科技有限公司 | Bacterium or viral online acquisition and on-line automatic detection device in a kind of air |
| CN107118951A (en) * | 2017-05-16 | 2017-09-01 | 上海德具生物科技有限公司 | One kind sampling integrated sampling box structure |
| CN106967595B (en) * | 2017-05-16 | 2019-11-29 | 上海德具生物科技有限公司 | Bacterium or viral online acquisition and online automatic detection method in a kind of air |
| CN107118951B (en) * | 2017-05-16 | 2020-06-05 | 江苏博凯生物科技有限公司 | Sampling integration sampling box structure |
| CN107118961B (en) * | 2017-05-16 | 2020-06-16 | 上海德具生物科技有限公司 | Online collection and online automatic detection device for bacteria or viruses in air |
| CN108426751A (en) * | 2018-01-23 | 2018-08-21 | 宁波市环境监测中心 | A kind of gas sampling assembly and its method |
| CN108426751B (en) * | 2018-01-23 | 2023-11-14 | 宁波市环境监测中心 | Gas sampling device and method thereof |
| CN114279775A (en) * | 2021-12-29 | 2022-04-05 | 军事科学院***工程研究院卫勤保障技术研究所 | Sampling device for monitoring bioaerosol |
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| CN102559486B (en) | 2014-03-19 |
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