CN102550288A - Edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags - Google Patents
Edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags Download PDFInfo
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- CN102550288A CN102550288A CN2012100132410A CN201210013241A CN102550288A CN 102550288 A CN102550288 A CN 102550288A CN 2012100132410 A CN2012100132410 A CN 2012100132410A CN 201210013241 A CN201210013241 A CN 201210013241A CN 102550288 A CN102550288 A CN 102550288A
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Abstract
Disclosed is an edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags. The method is characterized that during the production of the stock strain or culture strain, the process comprises preparing raw materials, which comprises preparing wheat grains, sawdust and cottonseed shell as culture medium for stock strain, or preparing wheat grains, sawdust and cottonseed shell as culture medium for culture strain, and preparing moisture-proof covers and plenty of cotton plugs for later use; feeding the culture medium into strain bottles or culture bags with conventional methods, inserting cotton plugs into bottle mouths or opening of the culture bags, and covering the moisture-proof covers at the bottle mouths or bag openings to form moisture-proof cover layers for preventing cotton plugs from being moistened, sterilizing the culture medium by atmospheric pressure steam sterilization or high pressure steam sterilization; and inoculating and culturing to obtain stock strain or culture strain. The method of the invention can completely prevent moisture absorption phenomenon, the operation is simple, the moisture-proof covers can be repeatedly used, the use cost is low, the problem of moisture absorption of the cotton plugs can be well solved, Neurospora growth on the cotton plugs after inoculation can be prevented, and contamination of the fungus strains is avoided.
Description
(1), technical field
The present invention relates to a kind of edible fungus species manufacturing technology, particularly a kind of edible fungus species (referring to original seed or cultivated species) preparation method that can prevent cotton plug humidity in seed bottle or the culture bag.
(2), background technology
As everyone knows, the successful key of artificial cultivation edible mushroom is a bacterial classification, has only good bacterial classification just can reach good quality and high output, and edible fungus species manufacturing process comprises: first stage is to produce female the kind, and just purification and rejuvenation obtain female the kind from fruit body; Second stage is to produce original seed, just utilizes female mycelium of planting to insert in the pedigree seed culture medium, and expanding propagation is cultivated into original seed (being also referred to as the secondary kind); Three phases is to produce cultivated species, has had original seed not enough, also will expand it to cultivated species i.e. " three grades of kinds " in the phase III, and cultivated species is exactly the mycelium bacterial classification (become the bacterial classification that can directly put) of expanding propagation after once again that utilizes original seed.Because in large-scale bacterial classification manufacturing process, as in seed bottle that the medium of original seed or cultivated species is packed into or with the medium of cultivated species, packing into when being used for the operation of the culture bag that bacterial classification makes, and in the operating process of inoculation (sowing) etc.; All be difficult to the sterile working state that reaches real,, improve the bacterial classification yield rate in order to reduce the bacterial classification pollution rate; In each stage that bacterial classification is made, people have taked the many measure or methods that can reduce the bacterial classification pollution rate, as at present in the bacterial classification manufacturing process of (referring to original seed or cultivated species); People also adopt the method for medium being carried out steam sterilizing usually, just in seed bottle (like the original seed bottle) or be used for culture bag that bacterial classification makes and pack into behind the medium, have filled in cotton plug at the sack place of bottle mouth position or culture bag again; When adopting normal pressure or high pressure steam sterilization, the moist phenomenon of cotton plug often takes place, and (concrete reason is after the high-temperature steam sterilization finishes cooling, can produce condensed water at bottle mouth position (or sack place of culture bag); Make cotton plug moisturize); For solving this difficult problem, generally be that to adopt material such as disregard message paper that cotton plug is wrapped the prevention cotton moist and receive water, adopt this method moistureproof; Though can reach certain moisture effect; But because there is the halfway trouble of moisture effect, and often produce living contaminants phenomenon (growing Neurospora on the cotton plug), seriously restrict and have influence on survival rate and yield rate that bacterial classification is made; Reach 50% and pollute, even up to more than 100%.Also seriously restrict and influenced productivity effect.
(3), summary of the invention
Task of the present invention provides a kind of edible fungus species preparation method (promptly utilizing dampproof cover to make the method that the moisture proof capping layer is made edible fungus species (original seed or cultivated species)) that can prevent cotton plug humidity in seed bottle or the culture bag; Make and to play prevention and prevent the moist water of receiving of cotton plug; It is good that bacterial classification is exempted from pollution effects; Can at utmost overcome manufacturing behind normal pressure or high pressure steam sterilization and produce the living contaminants phenomenon in the process; Obviously improve survival rate and the yield rate that bacterial classification is made, and this method processing ease, processing cost is light.
The present invention realizes with following technical scheme; This edible fungus species preparation method that can prevent cotton plug humidity in seed bottle or the culture bag of the present invention; Can be used for making bacterial classifications such as wheat, wood chip, cotton seed hulls (promptly comprising cultivated speciess such as original seeds such as making wheat, wood chip, cotton seed hulls and wheat, wood chip, cotton seed hulls); This method still comprises following processing step: the one, produce female the kind (claiming the one-level kind again); Just purification and rejuvenation obtain female kind, the 2nd from fruit body, produce (wheat etc.) original seed; Just utilize female mycelium of planting to insert in the pedigree seed culture medium, expanding propagation is cultivated into original seed (claiming the secondary kind again), the 3rd, utilizes original seed to produce cultivated species (like cultivated speciess such as wheats, claiming three grades of kinds again); With as producing with cultivated species (being the bacterial classification that can directly put into production); It is characterized in that: wherein saidly producing original seed or cultivated species when (comprising cultivated speciess such as original seed such as wheat or wheat), the processing step of its making is: get the raw materials ready, comprise the processing and fabricating medium; Promptly comprise original seeds such as processing and fabricating wheat, wood chip, cotton seed hulls or cultivated speciess such as wheat, wood chip, cotton seed hulls medium is subsequent use, and processing and manufacturing plastics dampproof cover (adopting polypropylene plastics processing) and to get the q.s cotton plug subsequent use; According to conventional method with in medium medium such as () the wheats seed bottle of packing into (can select the blake bottle or the original seed bottle of 750 milliliters of specifications for use) or be used for the culture bag that bacterial classification is made; Fill in cotton plug from bottle mouth position afterwards, or fill in cotton plug again after outside the sack of culture bag, putting a collar; Cover dampproof cover as the moisture proof capping layer (separation layer effect) that prevents that cotton plug is moist from the sack of bottle mouth position or culture bag more afterwards; Afterwards the medium in seed bottle or the culture bag is adopted normal pressure or high pressure steam sterilization; Inoculated and cultured; Be to insert female kind (claim not only one-level kind) or original seed (but also claiming the secondary kind) after the sterilization treatment; To connect female plant or the seed bottle of original seed or culture bag are positioned in the culturing room to cultivate according to conventional method and can become original seed or cultivated species (is used to make cultivated species after wherein original seed is carried out afterwards; After wherein cultivated species was carried out, the bacterial classification that just can become the usefulness that directly puts into production was used for having sent out the bacterium fruiting).
The present invention in the specific implementation, its above-mentioned technical characterictic can further be specially:
1, described dampproof cover should be to process can correspondingly with seed bottle or culture bag (be used for bacterial classification and make usefulness) cooperate or the specification (promptly should note the big or small required plastics dampproof cover of making according to the size or the culture bag sack place shape of seed bottle size of vial) of corresponding pairing.
2, described dampproof cover is a bottle cap type structure of processing the conventional tubbiness shape or " U " type of falling.
3, when selecting culture bag for use as the culture vessel (bag) of edible fungus species (referring to original seed or cultivated species); Also socket (overcoat) has the plastics collar (the short collar) that can match with said dampproof cover near described culture bag sack place, and the external diameter of this plastics collar should be suitable with the internal diameter of the dampproof cover of said tubbiness shape or " fall U " type.
Operation principle of the present invention and beneficial effect are: the dampproof cover that the present invention adopts polypropylene plastics to be processed into, in bacterial classification (referring to cultivated species) manufacturing process, after the sack place of bottle mouth position or culture bag covers dampproof cover; Dampproof cover can prevent thoroughly that the cotton plug moisture absorption phenomenon from taking place, and utilize these plastics dampproof covers directly by being pressed on the bottleneck cotton plug or forming capping layer (playing the separation layer effect) on the cotton plug of culture bag sack; Moistureproof simple to operate; And the plastics dampproof cover can the repeated multiple times utilization, uses economically, and cost is light; Moisture effect is good; The difficult problem of the cotton plug moisture absorption in can fine solution high-temperature steam sterilization process prevents to grow Neurospora after the inoculation, causes bacterial classification all to pollute.In a word, the present invention can at utmost overcome manufacturing behind normal pressure or high pressure steam sterilization and produce the living contaminants phenomenon in the process, obviously improves survival rate and yield rate (reaching 100%) that bacterial classification is made, and this method processing ease, and processing cost is light.
(4), description of drawings
Fig. 1 is the structural representation of the plastics dampproof cover described in the present invention.
Fig. 2 is the structural representation that covers the plastics dampproof cover the present invention puts the collar outside the sack place of culture bag after again.
Fig. 3 is the present invention covers a plastics dampproof cover at the bottle mouth position of seed bottle a structural representation.
In the accompanying drawings: 1～tubbiness shape or " U " plastic dampproof cover lid that falls, 2～culture bag, 3～collar, the sack place of 4～culture bag, 5～cotton plug, 6～medium, 7～seed bottle, the bottle mouth position of 8～seed bottle.
(5), embodiment
It below is a kind of specific embodiment that utilizes technical scheme of the present invention; This preparation method that can prevent the edible fungus species (referring to original seed or cultivated species) that cotton plug is moist in seed bottle or the culture bag of the present invention; Can be used for making bacterial classifications such as wheat (promptly comprising cultivated speciess such as original seeds such as making wheat and wheat); This method still comprises following processing step: the one, produce female the kind (claiming the one-level kind again); Just purification and rejuvenation obtain female kind, the 2nd from fruit body, produce (wheat etc.) original seed; Just utilize female mycelium of planting to insert in the pedigree seed culture medium, expanding propagation is cultivated into original seed (claiming the secondary kind again), the 3rd, utilizes original seed to produce cultivated species (like cultivated speciess such as wheats, claiming three grades of kinds again); With as producing with cultivated species (being the bacterial classification that can directly put into production); It is characterized in that: wherein said when producing original seed or cultivated species (cultivated speciess such as original seed such as wheat or wheat), the processing step of its making is: get the raw materials ready, comprise the processing and fabricating medium; Promptly comprise original seeds such as processing and fabricating wheat, wood chip, cotton seed hulls or cultivated speciess such as wheat, wood chip, cotton seed hulls medium is subsequent use, and processing and manufacturing plastics dampproof cover (adopting polypropylene plastics processing) and to get the q.s cotton plug subsequent use; According to conventional method with in (can select the blake bottle or the original seed bottle of 750 milliliters of specifications for use) or the culture bag in medium (medium such as wheat) seed bottle of packing into (selecting specification 17*33*0.05cm for use); Fill in cotton plug from bottle mouth position afterwards, or fill in cotton plug again after outside the sack of culture bag, putting a collar; Cover dampproof cover from the sack of bottle mouth position or culture bag more afterwards and seal the back as the moisture proof capping layer that prevents that cotton plug is moist; Afterwards the medium in seed bottle or the culture bag is adopted normal pressure or high pressure steam sterilization; Inoculated and cultured; Be to insert female kind (claim not only one-level kind) or original seed (but also claiming the secondary kind) after the sterilization treatment; To connect female plant or the seed bottle of original seed or culture bag are positioned in the culturing room to cultivate according to conventional method and can become original seed or cultivated species (is used to make cultivated species after wherein original seed is carried out afterwards; After wherein cultivated species was carried out, the bacterial classification that just can become the usefulness that directly puts into production was used for having sent out the bacterium fruiting).
The present invention in the specific implementation, its above-mentioned technical characterictic can further be specially:
1, described dampproof cover should be to process can correspondingly with seed bottle bottleneck or culture bag sack cooperate or the specification (promptly should note the big or small required plastics dampproof cover of making according to the size or the culture bag sack place shape of seed bottle size of vial) of corresponding pairing.
2, described dampproof cover is a bottle cap type structure of processing the conventional tubbiness shape or " U " type of falling.
3, when selecting culture bag for use as the culture vessel (bag) of edible fungus species (referring to original seed or cultivated species); Also socket (overcoat) has the plastics collar (the short collar) that can match with said dampproof cover near described culture bag sack place, and the external diameter of this plastics collar should be suitable with the internal diameter of the dampproof cover of said tubbiness shape or " fall U " type.
Claims (4)
1. one kind can prevent the moist edible fungus species preparation method of cotton plug in seed bottle or the culture bag; Can be used for making wheat, wood chip, cotton seed hulls original seed and wheat, wood chip, cottonseed shell cultivation kind; This method comprises following processing step: the one, and produce mother and plant, just purification and rejuvenation obtain female kind, the 2nd from fruit body, produce original seed, just utilize in female mycelium access pedigree seed culture medium of planting; Expanding propagation is cultivated into original seed, the 3rd, utilizes original seed to produce cultivated species; It is characterized in that: wherein said when producing original seed or cultivated species, the processing step of its making is: get the raw materials ready, comprise the processing and fabricating medium; Promptly comprise original seeds such as processing and fabricating wheat, wood chip, cotton seed hulls or cultivated speciess such as wheat, wood chip, cotton seed hulls medium is subsequent use, and the moistureproof vinyl cover of processing and manufacturing is with to get the q.s cotton plug subsequent use; According to conventional method medium is packed in seed bottle or the culture bag, fill in cotton plug from bottle mouth position afterwards, or fill in cotton plug again after outside the sack of culture bag, putting a collar; Cover dampproof cover as the moisture proof capping layer that prevents that cotton plug is moist from the sack of bottle mouth position or culture bag more afterwards; Afterwards the medium in seed bottle or the culture bag is adopted normal pressure or high pressure steam sterilization; Inoculated and cultured promptly inserts female the kind or original seed after the sterilization treatment, will connect female plant or the seed bottle of original seed or culture bag are positioned in the culturing room to cultivate according to conventional method and can become original seed or cultivated species afterwards.
2. the edible fungus species preparation method that can prevent cotton plug humidity in seed bottle or the culture bag according to claim 1; It is characterized in that: described dampproof cover should be to process can correspondingly with seed bottle bottleneck or culture bag sack cooperate or the specification of corresponding pairing, promptly should note the big or small required plastics dampproof cover of making according to the size or the culture bag sack place shape of seed bottle size of vial.
3. the edible fungus species preparation method that can prevent cotton plug humidity in seed bottle or the culture bag according to claim 1 and 2 is characterized in that: described dampproof cover is a bottle cap type structure of processing the conventional tubbiness shape or " U " type of falling.
4. the edible fungus species preparation method that can prevent cotton plug humidity in seed bottle or the culture bag according to claim 1 and 2; It is characterized in that: when selecting culture bag for use as the culture vessel of edible fungus species; Near described culture bag sack place, also be socketed with the plastics collar that can match with said dampproof cover, the external diameter of this plastics collar should be suitable with the internal diameter of the dampproof cover of said tubbiness shape or " fall U " type.
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| CN2012100132410A CN102550288A (en) | 2012-01-09 | 2012-01-09 | Edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags |
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| CN2012100132410A CN102550288A (en) | 2012-01-09 | 2012-01-09 | Edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845219A (en) * | 2012-09-06 | 2013-01-02 | 西北农林科技大学 | Cultivation technique of Pleurotus eryngii |
| CN114885754A (en) * | 2022-06-13 | 2022-08-12 | 贵州省山地资源研究所 | Method for manufacturing sealing plug of dictyophora phalloidea cultivated species |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101584286A (en) * | 2009-06-15 | 2009-11-25 | 郑雪平 | Edible fungus cluster producing art |
| CN102173883A (en) * | 2011-02-14 | 2011-09-07 | 天津鸿滨禾盛农业技术开发有限公司 | New compost formula for industrial cultivation of pleurotus eryngii |
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2012
- 2012-01-09 CN CN2012100132410A patent/CN102550288A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101584286A (en) * | 2009-06-15 | 2009-11-25 | 郑雪平 | Edible fungus cluster producing art |
| CN102173883A (en) * | 2011-02-14 | 2011-09-07 | 天津鸿滨禾盛农业技术开发有限公司 | New compost formula for industrial cultivation of pleurotus eryngii |
Non-Patent Citations (2)
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| 李宏伟、孙永琴: "《黑木耳代料栽培技术》", 31 March 2001, 东北林业大学出版社 * |
| 魏鹏、宋天凤: "食用菌菌种的制作", 《新疆农机化》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845219A (en) * | 2012-09-06 | 2013-01-02 | 西北农林科技大学 | Cultivation technique of Pleurotus eryngii |
| CN102845219B (en) * | 2012-09-06 | 2013-11-06 | 西北农林科技大学 | Cultivation technique of Pleurotus eryngii |
| CN114885754A (en) * | 2022-06-13 | 2022-08-12 | 贵州省山地资源研究所 | Method for manufacturing sealing plug of dictyophora phalloidea cultivated species |
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Application publication date: 20120711 |