CN106048744A - Method for preparing extracellular matrix-simulated nanometer fiber dressing through electrostatic spinning - Google Patents
Method for preparing extracellular matrix-simulated nanometer fiber dressing through electrostatic spinning Download PDFInfo
- Publication number
- CN106048744A CN106048744A CN201610479265.3A CN201610479265A CN106048744A CN 106048744 A CN106048744 A CN 106048744A CN 201610479265 A CN201610479265 A CN 201610479265A CN 106048744 A CN106048744 A CN 106048744A
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- Prior art keywords
- zein
- solution
- electrostatic spinning
- collagen
- extracellular matrix
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Classifications
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Mechanical Engineering (AREA)
- Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a method for preparing extracellular matrix-simulated nanometer fiber dressing through electrostatic spinning. Zein and collagen are used as raw materials, and solvent is a volatile liquid, such as acetic acid or ethanol. A collagen solution and a zein solution are obtained by respectively dissolving collagen sponge and the zein. The collagen solution and the zein solution are mixed according to a certain proportion, and the mixture is subjected to electrostatic spinning to obtain composite nanometer fiber film. A certain amount of medicament is weighed to be mixed with a spinning liquid to obtain a fiber film containing medicament. The composite fiber film has better mechanical properties, biological compatibility and degradability in vivo and vitro. The medicament is wrapped in the fiber and gradually released as the fiber gradually degrading to prevent burst release of the medicament. Simulation of extracellular matrix structure provides optimal external environment for cell growth and tissue regeneration. The preparation method is simple, and industrialized production is easy to realize.
Description
[technical field]: the invention belongs to the preparation field of composite nano fiber, is specifically related to one and utilizes Static Spinning
The method of the imitative extracellular matrix nanofiber dressing of silk preparation.
[background technology]: organizational project is to combine biology and engineering principles, studies, prepares and apply biosubstitute
To repair and to improve an emerging science of damaged tissues.Histiocyte is combined in vitro with biomaterial, has been built into life
" complex " of life activity, and " complex " implanted pathological changes, defective tissue position, repair and guide tissue regeneration." plant
Daughter cell ", timbering material and somatomedin be the three elements constituting " complex ", wherein prepare a kind of biocompatibility good
The three-dimensional cell growth timbering material problem that becomes most critical.
Electrostatic spinning technique is a kind of important method utilizing high voltage electric field to prepare nanofiber.At biomedical sector,
The diameter of nanofiber is less than cell, can simulate structure and the biological function of natural extracellular matrix, and by Static Spinning
The fibrous membrane of silk preparation has the advantages such as porosity is high, specific surface area is big, fibre diameter is controlled, considers from many aspects, nanometer
The optimum selection that fiber scaffold material is cell seeding, grows and breeds.
Zein (Zein) is the storage protein of a kind of Semen Maydis, has the spinnability of uniqueness, dissolution characteristics, heat-resisting
The advantages such as property, film property, non-oxidizability and biocidal property.Electrospinning Zein nanofiber is owing to having excellent biocompatibility, life
The features such as thing degradability, excellent in mechanical performance, are widely used in field of tissue engineering technology.The Zhang Mei et al. the side by electrostatic spinning
Method, with zein and polyvinyl alcohol as raw material, is prepared for a kind of cell culture material；Chinese patent
CN102587036A discloses " preparation method of a kind of corn alcohol-soluble protein nanofiber membrane cultivated for cell ", is with second
The aqueous solution of alcohol is the nanofiber that solvent is prepared for good biocompatibility, good stability.Zein is by substantial amounts of hydrophobic amino acid
Composition, lacks the essential amino acids such as lysine, tryptophan, and single Zein nanofiber is of low nutritive value, and application is restricted.
Natural extracellular matrix is made up of protein and the polysaccharide of Nanofiber Network structure, it would be desirable to add other natural one-tenth
Divide to meet the needs of analog cell epimatrix completely in composition structure.
Collagen protein (collagen) is the basic structure component of extracellular matrix, accounts for the 30% of body protein tissue, point
Subchain is by hydrogen bond together with covalent linkage, and collagen chain can be self-assembled into stable fiber, this high-sequential
Structure can make natural collagen substrate show higher mechanical strength.Additionally, collagen protein has biological degradability, high biofacies
Capacitive, and permeability and the wound repair of cell can be improved, it is 460nm that type i collagen solution is prepared as average diameter by Rho etc.
Fibrous material, and compound cells carries out cell cultivation, result show material promote human epidermal cell, the adhesion of Stomatocyte and
Propagation, further animal experimental observation finds that fibrinous tissue debris occurs in Mus wound covered fiber material one week after, sees
Observe fibroblastic propagation.Collagen protein has caused the extensive pass of research worker as a kind of biomedical matrix material
Note.
[summary of the invention]: it is multiple that the technical problem to be solved is to provide a kind of zein/collagen protein
Close the preparation method that nanofiber imitates the nano fibrous membrane of extracellular matrix, prepared composite fibre film dynamic performance and degradability
Can be excellent, this composite fibre can be good at simulating the Nomenclature Composition and Structure of Complexes of human body cell epimatrix, grows for cell and organizes
The bionical physiological environment that raw offer is optimal, is preferable tissue engineering bracket；Drug particles can be coated on fibrous inside, with fibre
Dimension homogeneous blend, reaches the effect that medicine slowly discharges；And this preparation method is simple and convenient, raw material sources is extensive, easily
Industrialization is realized in expanding production.
For reaching this purpose, the technology path that the present invention uses is:
1) prepare collagen solution: be dissolved in glacial acetic acid by collagen protein, stir to being completely dissolved under room temperature, obtain dense
Degree is the collagen solution of 15%-30%；
2) prepare zein solution: be dissolved in glacial acetic acid by zein, stir to the most molten under room temperature
Solve, obtain the zein solution that concentration is 25%-35%；
3) mixed solution of zein and collagen protein is prepared: by collagen solution according to accounting for the molten egg of Semen Maydis alcohol
White solution quality mixes than 15%-30%, stirs, and obtains electrostatic spinning liquid is blended；
4) medicine carrying is carried out: weigh separation of naproxen sodium and account for the amount of zein solution mass ratio 10%-25%, be added to
In blend spinning liquid, stir, obtain the co-blended spinning liquid of pastille；
5) electrospinning parameters is arranged: zein and collagen protein mixed solution are carried out electrostatic spinning, adjusts
Electrostatic spinning process parameter, control voltage is 17-20kV, and electric field distance is 10-20cm, and spinning speed is 0.4-1.0mL/h,
To zein/collagen protein composite nano-fiber membrane.
6) after being taken off from reception plate by fibrous membrane, being placed in vacuum drying oven, room temperature places 48h.
The technical scheme principle that the present invention solves to be provided is: select zein and collagen protein as main
Spinning material, utilizes the Nomenclature Composition and Structure of Complexes of Electrospun nano-fibers this structural simulation human body cell epimatrix, raw for cell
Long and tissue regeneration provides optimal bionical physiological environment, is preferable tissue engineering bracket；Coating medicine can be along with fiber
The degraded of film slowly discharges, and applies as dressing.
Mechanical property and the degradation property of the zein/collagen protein composite fiber of the present invention are excellent, and this system
Preparation Method is simple and convenient, and raw material sources is extensive, it is easy to expanding production realizes industrialization.
[accompanying drawing explanation]
Fig. 1 is Zein nano fiber film stereoscan photograph
Fig. 2 is zein/collagen protein composite nano-fiber membrane stereoscan photograph
Fig. 3 is the composite nano-fiber membrane stereoscan photograph of medicine carrying
Fig. 4 is that l cell cultivates propagation stereoscan photograph on nano fibrous membrane
[specific embodiments]
Embodiment 1:
1) prepare collagen solution: be dissolved in glacial acetic acid by collagen protein, stir to being completely dissolved under room temperature, obtain dense
Degree is the collagen solution of 10%；
2) prepare zein solution: be dissolved in glacial acetic acid by zein, stir to the most molten under room temperature
Solve, obtain the zein solution that concentration is 30%；
3) mixed solution of zein and collagen protein is prepared: by collagen solution according to accounting for the molten egg of Semen Maydis alcohol
White solution quality, than 10% mixing, stirs, and obtains electrostatic spinning liquid is blended；
4) carry out medicine carrying: weigh separation of naproxen sodium and account for the amount of zein solution mass ratio 10%, be added to mixing
In spinning liquid, stir, obtain the co-blended spinning liquid of pastille；
5) electrospinning parameters is arranged: zein and collagen protein mixed solution are carried out electrostatic spinning, adjusts
Electrostatic spinning process parameter, control voltage is 18kV, and electric field distance is 15cm, and spinning speed is 0.6mL/h, obtains collagen egg
In vain/zein composite nano-fiber membrane.
6) after being taken off from reception plate by fibrous membrane, being placed in vacuum drying oven, room temperature places 48h.
Embodiment 2:
1) collagen solution is prepared.Collagen protein is dissolved in glacial acetic acid, stirs to being completely dissolved under room temperature, obtain dense
Degree is the collagen solution of 20%；
2) zein solution is prepared.With embodiment 1.
3) mixed solution of zein and collagen protein is prepared.By collagen solution according to accounting for the molten egg of Semen Maydis alcohol
White solution quality, than 20% mixing, stirs, and obtains electrostatic spinning liquid is blended；
4) carry out medicine carrying: weigh separation of naproxen sodium and account for the amount of zein solution mass ratio 15%, be added to mixing
In spinning liquid, stir, obtain the co-blended spinning liquid of pastille；
5) electrospinning parameters is arranged.With embodiment 1.
6) after being taken off from reception plate by fibrous membrane, being placed in vacuum drying oven, room temperature places 48h.
Embodiment 3:
1) collagen solution is prepared.Collagen protein is dissolved in glacial acetic acid, stirs to being completely dissolved under room temperature, obtain dense
Degree is the collagen solution of 25%；
2) zein solution is prepared.With embodiment 1.
3) mixed solution of zein and collagen protein is prepared.By collagen solution according to accounting for the molten egg of Semen Maydis alcohol
White solution quality, than 25% mixing, stirs, and obtains electrostatic spinning liquid is blended；
4) carry out medicine carrying: weigh separation of naproxen sodium and account for the amount of zein solution mass ratio 20%, be added to mixing
In spinning liquid, stir, obtain the co-blended spinning liquid of pastille；
5) electrospinning parameters is arranged.With embodiment 1.
6) after being taken off from reception plate by fibrous membrane, being placed in vacuum drying oven, room temperature places 48h.
Embodiment 4:
1) collagen solution is prepared.Collagen protein is dissolved in glacial acetic acid, stirs to being completely dissolved under room temperature, obtain dense
Degree is the collagen solution of 30%；
2) zein solution is prepared.With embodiment 1.
3) mixed solution of zein and collagen protein is prepared.By collagen solution according to accounting for the molten egg of Semen Maydis alcohol
White solution quality, than 30% mixing, stirs, and obtains electrostatic spinning liquid is blended；
4) carry out medicine carrying: weigh separation of naproxen sodium and account for the amount of zein solution mass ratio 25%, be added to mixing
In spinning liquid, stir, obtain the co-blended spinning liquid of pastille；
5) electrospinning parameters is arranged.With embodiment 1.
6) after being taken off from reception plate by fibrous membrane, being placed in vacuum drying oven, room temperature places 48h.
Claims (6)
1. the method utilizing the imitative extracellular matrix nanofiber dressing of method of electrostatic spinning preparation, comprises the steps:
1) preparing collagen solution: be dissolved in glacial acetic acid by collagen protein, stir to being completely dissolved under room temperature, obtaining concentration is
The collagen solution of 15%-30%；
2) prepare zein solution: be dissolved in glacial acetic acid by zein, stir under room temperature to being completely dissolved,
It is the zein solution of 25%-35% to concentration；
3) mixed solution of collagen protein and zein is prepared: collagen solution is molten according to accounting for zein
Liquid mass ratio 15%-30% mixes, and stirs, and obtains electrostatic spinning liquid is blended；
4) medicine carrying is carried out: weigh separation of naproxen sodium etc. and account for the amount of zein solution mass ratio 10%-25%, be added to mix
Close in spinning liquid, stir, obtain the co-blended spinning liquid of pastille；
5) electrospinning parameters is arranged: be injected in syringe by spinning liquid, carries out electrostatic spinning after installation, adjusts electrostatic
Spinning technology parameter, control voltage is 17-20kV, and electric field distance is 10-20cm, and spinning speed is 0.4-1.0mL/h, obtains jade
Rice prolamin/collagen protein composite nano-fiber membrane.
6) after being taken off from reception plate by fibrous membrane, being placed in vacuum drying oven, room temperature places 48h.
A kind of side utilizing the imitative extracellular matrix nanofiber dressing of method of electrostatic spinning preparation the most according to claim 1
Method, it is characterised in that: electrostatic spinning solution solvent is glacial acetic acid solution, volatile.
A kind of side utilizing the imitative extracellular matrix nanofiber dressing of method of electrostatic spinning preparation the most according to claim 1
Method, it is characterised in that: in electrostatic spinning solution, the amount of zein is to immobilize, the ratio that regulation collagen protein is different
Example.
A kind of side utilizing the imitative extracellular matrix nanofiber dressing of method of electrostatic spinning preparation the most according to claim 1
Method, it is characterised in that: in electrostatic spinning solution, collagen protein accounts for the 15%-30% of zein mass fraction, Semen Maydis alcohol
Molten albumen accounts for the 25%-35% of solute total mass fraction.
A kind of side utilizing the imitative extracellular matrix nanofiber dressing of method of electrostatic spinning preparation the most according to claim 1
Method, it is characterised in that: described solute is the medicines such as zein and collagen protein, the optional naproxen sodium of slow releasing pharmaceutical.
A kind of side utilizing the imitative extracellular matrix nanofiber dressing of method of electrostatic spinning preparation the most according to claim 1
Method, it is characterised in that: the diameter of described collagen protein/zein composite nano fiber increases with spinning solution concentration
It is gradually increased, is gradually reduced with the increase of collagen content.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109908110A (en) * | 2019-03-27 | 2019-06-21 | 西安工程大学 | A kind of two-layer compound carries medicine adherency patch and its preparation method and application |
| CN110433325A (en) * | 2019-07-26 | 2019-11-12 | 杭州中科润德生物技术发展有限公司 | A kind of protide high polymer nanometer fiber hemostatic material and its preparation method and application |
| CN111850731A (en) * | 2020-07-24 | 2020-10-30 | 吉林农业大学 | Alcohol soluble protein-based core-shell fiber film, food storage and preservation material and preparation method |
| CN112891537A (en) * | 2021-02-08 | 2021-06-04 | 沈阳药科大学 | Photoelectric spinning fibrous membrane with anti-tumor function and preparation method and application thereof |
| CN113089185A (en) * | 2021-04-02 | 2021-07-09 | 南通大学 | Conductive nanofiber membrane with sterilization function and preparation method and application thereof |
| CN113144271A (en) * | 2021-04-22 | 2021-07-23 | 天津工业大学 | Preparation method of medical dressing with medicine carrying function |
| CN113913360A (en) * | 2020-07-07 | 2022-01-11 | 诺一迈尔(苏州)生命科技有限公司 | Fibrous membrane for in vitro cell culture |
| CN115380999A (en) * | 2022-08-12 | 2022-11-25 | 北京东方天合生物技术有限责任公司 | Rumen-bypass melatonin composition for improving pregnancy rate of ruminants as well as preparation method and application of rumen-bypass melatonin composition |
| CN115804867B (en) * | 2022-12-29 | 2023-12-05 | 深圳市拉菲尔生物医药有限公司 | Fibrotic type III collagen nanomembrane, preparation method and application thereof in skin regeneration |
| CN117802697A (en) * | 2024-03-01 | 2024-04-02 | 中国农业大学 | Method for improving mechanical strength of zein nanofiber membrane |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109908110A (en) * | 2019-03-27 | 2019-06-21 | 西安工程大学 | A kind of two-layer compound carries medicine adherency patch and its preparation method and application |
| CN110433325A (en) * | 2019-07-26 | 2019-11-12 | 杭州中科润德生物技术发展有限公司 | A kind of protide high polymer nanometer fiber hemostatic material and its preparation method and application |
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| CN111850731A (en) * | 2020-07-24 | 2020-10-30 | 吉林农业大学 | Alcohol soluble protein-based core-shell fiber film, food storage and preservation material and preparation method |
| CN112891537A (en) * | 2021-02-08 | 2021-06-04 | 沈阳药科大学 | Photoelectric spinning fibrous membrane with anti-tumor function and preparation method and application thereof |
| CN113089185A (en) * | 2021-04-02 | 2021-07-09 | 南通大学 | Conductive nanofiber membrane with sterilization function and preparation method and application thereof |
| CN113089185B (en) * | 2021-04-02 | 2022-06-17 | 南通大学 | Conductive nanofiber membrane with sterilization function and preparation method and application thereof |
| CN113144271A (en) * | 2021-04-22 | 2021-07-23 | 天津工业大学 | Preparation method of medical dressing with medicine carrying function |
| CN113144271B (en) * | 2021-04-22 | 2022-05-27 | 天津工业大学 | Preparation method of medical dressing with medicine carrying function |
| CN115380999A (en) * | 2022-08-12 | 2022-11-25 | 北京东方天合生物技术有限责任公司 | Rumen-bypass melatonin composition for improving pregnancy rate of ruminants as well as preparation method and application of rumen-bypass melatonin composition |
| CN115380999B (en) * | 2022-08-12 | 2023-08-29 | 北京东方天合生物技术有限责任公司 | Rumen bypass melatonin composition for improving pregnancy rate of ruminant animals as well as preparation method and application thereof |
| CN115804867B (en) * | 2022-12-29 | 2023-12-05 | 深圳市拉菲尔生物医药有限公司 | Fibrotic type III collagen nanomembrane, preparation method and application thereof in skin regeneration |
| CN117802697A (en) * | 2024-03-01 | 2024-04-02 | 中国农业大学 | Method for improving mechanical strength of zein nanofiber membrane |
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