CN102492766A - Agar culture medium and preparation method thereof - Google Patents
Agar culture medium and preparation method thereof Download PDFInfo
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- CN102492766A CN102492766A CN2011104140939A CN201110414093A CN102492766A CN 102492766 A CN102492766 A CN 102492766A CN 2011104140939 A CN2011104140939 A CN 2011104140939A CN 201110414093 A CN201110414093 A CN 201110414093A CN 102492766 A CN102492766 A CN 102492766A
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- lactose
- peptone
- extract powder
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Abstract
The invention discloses an agar culture medium and a preparation method thereof. Each 1,000 milliliters of nutrient solution comprises the following components by mass: 8 to 12 grams of peptone, 9 to 11 grams of lactose, 4 to 6 grams of beef extract powder, 13 to 17 grams of agar, 4 to 6 grams of sodium chloride, 0.025 to 0.035 gram of Chinese blue and 0.025 to 0.035 gram of rhodizonic acid. The preparation method comprises the following steps of: 1) dissolving 10 grams of peptone, 10 grams of lactose, 5 grams of beef extract powder, 15 grams of agar and 5 grams of sodium chloride in 1,000 milliliters of nutrient solution; 2) mixing uniformly, heating, boiling and dissolving fully; 3) regulating the pH value to be between 7.3+/-0.1, and adding 0.03 gram of Chinese blue and 0.03 gram of rhodizonic acid; 4) heating to the temperature of 115 DEG C, keeping the temperature for 15 minutes, cooling to the temperature of about 50 DEG C, and pouring into a disposable sterile plastic plate; and 5) performing sterile inspection, and refrigerating at the temperature of 2 and 8 DEG C for later use.
Description
Technical field
The present invention relates to a kind of agar culture technique field, nutrient agar of especially a kind of separation and Culture that is used for pathogen enterobacteria and preparation method thereof.
Background technology
The situation that this product uses on the market was observed as object bacteria bacterium colony too for a short time being unfavorable for, caused the omission phenomenon easily.
Summary of the invention
The object of the invention just provides a kind of nutrient agar and preparation method thereof, through increasing the nutritive substance of product, improves the recall rate of object bacteria, solves the little phenomenon of object bacteria bacterium colony.
In order to reach above-mentioned purpose of design, the technical scheme that the present invention adopts is following:
A kind of nutrient agar, its moity and total mass number are: in every 1000ml nutritive medium: peptone 8-12g, lactose 9-11g, beef extract powder 4-6g, agar 13-17g, sodium-chlor 4-6g, lokav 0.025-0.035g, rhodizonic acid 0.025-0.035g.
Preferably, its moity and total mass number are: in every 1000ml nutritive medium: peptone 10g, lactose 10g, beef extract powder 5g, agar 15g, sodium-chlor 5g, lokav 0.03g, rhodizonic acid 0.03g.
Preferably, the staple of said nutritive medium is amino acid, VITAMINs, growth factor.
A kind of making method of nutrient agar may further comprise the steps:
1) peptone 10g, lactose 10g, beef extract powder 5g, agar 15g, sodium-chlor 5g are dissolved in the 1000ml nutritive medium;
2) behind the mixing, heated and boiled is fully dissolved;
3) regulate pH to 7.3 ± 0.1, add lokav 0.03g, rhodizonic acid 0.03g.
4) be heated to 115 ℃, and keep 15min, be cooled to about 50 ℃, be poured in the disposable sterilized plastics plate;
5) behind the steriling test in 2 ℃ of-8 ℃ of environment refrigeration subsequent use.
Preferably, regulate pH to 7.3 ± 0.1 with 1N-10N sodium hydroxide or 1N-10N hydrochloric acid soln.
Its principle is: principle of work: beef extract powder and peptone provide required carbon source of bacterial growth and nitrogenous source as base nutrients matter in substratum; Sodium-chlor maintenance system osmotic pressure balance; Lactose is a fermentable carbon source, and lokav and rhodizonic acid are indicator, and agar is peptizer.
The bacterium that can reduce lactose grows in the above and produces acid and make the color of its bacterium colony be blue; The selectivity of this substratum shows mainly whether bacterium can utilize the acid of lactose product and the indicator color is changed; The unfermentable lactose of the bacterium of Salmonellas and Shigella, so the color of its bacterium colony is consistent with the substratum true qualities, shape is translucent; The bacterium of colibacillus realm can utilize lactose to produce acid, makes its colony colour be blue.
The beneficial effect of nutrient agar of the present invention and preparation method thereof is: the single bacterium colony size of object bacteria is improved; Similar products at home and abroad on year-on-year basis; The size of single bacterium colony is bigger by 50% than like product, makes object bacteria be more prone to observe, and has solved the problem of omission.The bacterium colony size is better than similar products at home and abroad; The time ratio like product of sample sentence read result is shorter.
Embodiment
Embodiment 1,
A kind of nutrient agar, its moity and total mass number are: in every 1000ml nutritive medium: peptone 8g, lactose 9g, beef extract powder 4g, agar 17g, sodium-chlor 6g, lokav 0.035g, rhodizonic acid 0.035g.
Embodiment 2
A kind of nutrient agar, its moity and total mass number are: in every 1000ml nutritive medium: peptone 12g, lactose 11g, beef extract powder 6g, agar 13g, sodium-chlor 4g, lokav 0.025g, rhodizonic acid 0.025g.
Embodiment 3
A kind of nutrient agar, its moity and total mass number are: in every 1000ml nutritive medium: peptone 10g, lactose 10g, beef extract powder 5g, agar 15g, sodium-chlor 5g, lokav 0.03g, rhodizonic acid 0.03g.
In the foregoing description, the staple of said nutritive medium is amino acid, VITAMINs, growth factor.
Embodiment 4
A kind of making method of nutrient agar may further comprise the steps:
1) peptone 12g, lactose 11g, beef extract powder 6g, agar 13g, sodium-chlor 4g are dissolved in the 1000ml nutritive medium;
2) behind the mixing, heated and boiled is fully dissolved;
3) regulate pH to 7.3 ± 0.1, add lokav 0.035g, rhodizonic acid 0.035g.
4) be heated to 115 ℃, and keep 15min, be cooled to about 50 ℃, be poured in the disposable sterilized plastics plate;
5) behind the steriling test in 2 ℃ of-8 ℃ of environment refrigeration subsequent use.
Embodiment 5
A kind of making method of nutrient agar may further comprise the steps:
1) peptone 8g, lactose 9g, beef extract powder 4g, agar 17g, sodium-chlor 6g are dissolved in the 1000ml nutritive medium;
2) behind the mixing, heated and boiled is fully dissolved;
3) regulate pH to 7.3 ± 0.1, add lokav 0.025g, rhodizonic acid 0.025g.
4) be heated to 115 ℃, and keep 15min, be cooled to about 50 ℃, be poured in the disposable sterilized plastics plate;
5) behind the steriling test in 2 ℃ of-8 ℃ of environment refrigeration subsequent use.
Embodiment 6
A kind of making method of nutrient agar may further comprise the steps:
1) peptone 10g, lactose 10g, beef extract powder 5g, agar 15g, sodium-chlor 5g are dissolved in the 1000ml nutritive medium;
2) behind the mixing, heated and boiled is fully dissolved;
3) regulate pH to 7.3 ± 0.1, add lokav 0.03g, rhodizonic acid 0.03g.
4) be heated to 115 ℃, and keep 15min, be cooled to about 50 ℃, be poured in the disposable sterilized plastics plate;
5) behind the steriling test in 2 ℃ of-8 ℃ of environment refrigeration subsequent use.
In the foregoing description, regulate pH to 7.3 ± 0.1 with 1N-10N sodium hydroxide or 1N-10N hydrochloric acid soln.
This embodiment the preferred embodiments of the present invention can not limit the present invention, and concrete each item rights protection scope is defined by the claims.
Claims (5)
1. nutrient agar, it is characterized in that: its moity and total mass number are: in every 1000ml nutritive medium: peptone 8-12g, lactose 9-11g, beef extract powder 4-6g, agar 13-17g, sodium-chlor 4-6g, lokav 0.025-0.035g, rhodizonic acid 0.025-0.035g.
2. nutrient agar according to claim 1 is characterized in that: its moity and total mass number are: in every 1000ml nutritive medium: peptone 10g, lactose 10g, beef extract powder 5g, agar 15g, sodium-chlor 5g, lokav 0.03g, rhodizonic acid 0.03g.
3. nutrient agar according to claim 1 and 2 is characterized in that: the staple of said nutritive medium is amino acid, VITAMINs, growth factor.
4. the making method of a nutrient agar is characterized in that: may further comprise the steps:
1) peptone 10g, lactose 10g, beef extract powder 5g, agar 15g, sodium-chlor 5g are dissolved in the 1000ml nutritive medium;
2) behind the mixing, heated and boiled is fully dissolved;
3) regulate pH to 7.3 ± 0.1, add lokav 0.03g, rhodizonic acid 0.03g.
4) be heated to 115 ℃, and keep 15min, be cooled to about 50 ℃, be poured in the disposable sterilized plastics plate;
5) behind the steriling test in 2 ℃ of-8 ℃ of environment refrigeration subsequent use.
5. the making method of nutrient agar according to claim 4 is characterized in that: regulate pH to 7.3 ± 0.1 with 1N-10N sodium hydroxide or 1N-10N hydrochloric acid soln.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104140939A CN102492766A (en) | 2011-12-13 | 2011-12-13 | Agar culture medium and preparation method thereof |
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| CN2011104140939A CN102492766A (en) | 2011-12-13 | 2011-12-13 | Agar culture medium and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816827A (en) * | 2012-08-10 | 2012-12-12 | 杭州澳亚生物技术有限公司 | Preparation method for culture medium product for medicine industry environment microorganism monitoring |
| CN103146799A (en) * | 2013-03-28 | 2013-06-12 | 中国人民解放军第三军医大学 | Kit for microbial detection |
-
2011
- 2011-12-13 CN CN2011104140939A patent/CN102492766A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 中华人民共和国***政司: "《全国临床检验操作规程（第二版）》", 31 December 1997, 东南大学出版社 * |
| 刘坚真 等: "国家标准测定食品细菌总数培养基的改进研究", 《微生物学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816827A (en) * | 2012-08-10 | 2012-12-12 | 杭州澳亚生物技术有限公司 | Preparation method for culture medium product for medicine industry environment microorganism monitoring |
| CN103146799A (en) * | 2013-03-28 | 2013-06-12 | 中国人民解放军第三军医大学 | Kit for microbial detection |
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Application publication date: 20120613 |