CN102419375B - Full-automatic chemoluminescence immunoassay analyzer - Google Patents
Full-automatic chemoluminescence immunoassay analyzer Download PDFInfo
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- CN102419375B CN102419375B CN 201110243705 CN201110243705A CN102419375B CN 102419375 B CN102419375 B CN 102419375B CN 201110243705 CN201110243705 CN 201110243705 CN 201110243705 A CN201110243705 A CN 201110243705A CN 102419375 B CN102419375 B CN 102419375B
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Abstract
The invention discloses a full-automatic chemoluminescence immunoassay analyzer. The analyzer is mainly composed of a working main body and a control computer connected with the working main body; a storage cabin and a control box are arranged under the working main body. The analyzer can be used for implementing the full-automatic operation of the immunoreaction process comprising reaction tube loading, sample automatic injection, reagent automatic injection, reaction solution incubation reaction, reaction solution automatic cleaning, reaction result detection and analysis; the full-automatic operation reduces the influence of human factors on the experiment, thereby improving the sensitivity. Each component of the invention is ingenious in design, the mutual cooperation of components isconvenient for the system integration so as to realize the automation; and based on the full-automatic chemoluminescence immunoassay analyzer, more devices with automatic function can be developed.
Description
Technical field
The present invention relates to a kind of immunity analysis instrument, particularly about a kind of full-automatic chemical luminescence immunoassay instrument.
Background technology
Immunology detection mainly is a kind of means of utilizing the specific reaction of antigen and antibody to detect, because it can utilize isotope, enzyme, chemiluminescent substance etc. that detection signal is amplified and show, therefore often be used to detect micro substances such as protein, hormone.Since the sixties in last century, immunoassay just is widely used in scientific research and clinical field.From the radioimmunology that begins most progressively develop into euzymelinked immunosorbent assay (ELISA), up to the chemiluminescence immunoassay method of present widespread use.
Chemiluminescence immune assay is that chemiluminescence or bioluminescence are combined with immune response, for detection of a kind of novel markings immunoassay of micro-antigen or antibody.Radio-immunity environmental protection, safety that the chemiluminescence immunoassay technology is more traditional, highly sensitive than enzyme linked immunological, sensing range is wide.Chemiluminescence immunoassay detects the generally approval that environmental protection, characteristics such as quick, accurate have obtained people, has become the main means of present clinical immunodiagnosis.
Nonetheless, chemiluminescence immune assay also has its restricted gender.The uncertainty of chemiluminescence immunoassay testing result comes from the influence of experimental implementation and detection architecture.In recent years, along with the progress of scientific and technological level, the stability, the consistance that detect reagent and testing instruments also progressively improve, and the principal element that influences the chemiluminescence detection result is exactly experimental implementation.Inspection body takes the mode that artificial experimental implementation, the luminous detector of semi-automatic chemistry detect mostly at present, and detecting instrument has only single read functions.There is individual difference in manually-operated, not only influences result's accuracy, has also limited the flux that detects when sample size is various.Can provide automation experiment flow operations, high-throughout chemiluminescence detector device to become the important topic that people face.
Summary of the invention
The object of the present invention is to provide a kind of full-automatic chemical luminescence immunoassay instrument, it can finish sequence of operations such as application of sample on the chemiluminescence immunoassay inspection process, reaction, data read automatically.
Purpose of the present invention is achieved through the following technical solutions: full-automatic chemical luminescence immunoassay instrument, comprise operative body, and connected control computing machine, below operative body, being provided with control box and storing storehouse, described operative body comprises:
Sample bin, described sample bin comprise by motor-driven controlled rotating circular disk and the bar code scanner of being located at the controlled rotating circular disk outside, and described bar code scanner is connected with the control computing machine by data line;
The reagent storehouse, described reagent storehouse comprises the kit that reagent glass stand and reagent cup constitute and the rotating disk that carries kit, and is provided with the temperature control mechanism that the kit activation temperature is provided and the reagent cup evenly mixing device that is used for reagent in the mixing reagent cup below described rotating disk; Described reagent cup evenly mixing device comprises electric rotating machine, is fixed with mixing on the output shaft of described electric rotating machine; And described reagent cup evenly mixing device connect control mixing near or away from the vortex mixer telecontrol equipment of reagent cup; Described mixing subcenter is provided with the projection piece, and periphery is provided with the card post; The bottom of cup of described reagent cup is provided with the recess of the cylindrical drum shape of mating with described projection piece, and the recess of described cylindrical drum shape extends radial spoke laterally and constitutes catch;
Reaction warehouse, described reaction warehouse comprises the go out reaction tube support of toothing of annulus, reaction tube support annulus outer radius be provided with filling reagent the reagent arm unit, be used for reaction tube is reclaimed the unit from ejector sleeve device, cleaning station, substrate filling apparatus, detecting unit and the waste liquid that the reaction tube support outside is pushed into reaction tube support inboard, be provided with the reaction tube evenly mixing device in reaction tube support bottom; Described reagent arm unit comprises automatic sample adding device and pin cleaning device, and described automatic sample adding device is made up of reagent needles, punch block, syringe pump and motion, and described reagent needles is installed in by punch block can be up and down or on the motion that swings; Described reagent needles connects T-valve, and described T-valve one end connection expresses pump, and the other end connects syringe pump; And the described pump that expresses is connected with the washing lotion bucket that carries cleaning fluid; Low cylinder, two concentric drums of outer high cylinder and connected waste liquid barrel in described pin cleaning device comprises, the low cylinder bottom of described inside be solid construction, is hollow-core construction bottom the high cylinder of described outside and is communicated with waste liquid barrel by pipeline; Described cleaning station comprises supporting construction, and priming device and liquid sucking device respectively are set on the described supporting construction; The below of described priming device arranges the reaction tube evenly mixing device with the reaction tube coupling; Install on the sidewall of described supporting construction can the adsorption reaction pipe in the adsorbent equipment of magnetic bead; And described priming device is connected the pump of control liquid absorption and rate of liquid aspiration with liquid sucking device; Described detecting unit comprises shutter, reaction tube suspension, detection means and transports reactor tube apparatus; The described reactor tube apparatus that transports is provided with the trustship structure of mating with reaction tube; Described reaction tube evenly mixing device comprises locator for fixation reaction pipe upper end, is positioned at mixing of reaction tube below, and control mixing to away from or the contact reaction tube side to mobile motion control mechanism, described mixing is connected with the mixing mechanism that is provided with electric rotating machine by eccentric structure.
Sampling/reaction tube apparatus for automatically loading, described sampling/reaction tube apparatus for automatically loading comprise that the sampling of being located between sample bin and the reaction warehouse moves the pipe arm and sampling moves the consumption material box conveyer that transports consumption material box of managing the arm below; Described consumption material box is provided with feed head and reaction tube, and described sampling moves pipe arm lower end and is provided with feed head stator and the reaction tube stator that is made of the step O-ring seal; Described consumption material box conveyer comprises lateral transfer portion and vertical delivery section, described lateral transfer portion and the vertical delivery section formation cross-shaped portion that intersects vertically, and described cross-shaped portion is provided with the inductor for induction consumption material box position.
The lateral transfer portion of described consumption material box conveyer is along the edge line setting of operative body; Comprise feed belt, be used for holding up the carrying roller of feed belt and the PTO that is connected with feed belt.
Vertical delivery section of described consumption material box conveyer is located between sample bin and the reaction warehouse, is positioned at the vertical lower that sampling moves the pipe arm; Vertical delivery section of described consumption material box conveyer comprises guiding thick stick, leading screw and transmission block, one end of the upper surface of described transmission block convexes to form the consumption material box pushing block, described transmission block is provided with two parallel screws, and described guiding thick stick and leading screw pass from described screw respectively.
Rotating disk in the described reagent storehouse comprises central circular boss dish and even some baffle arrangements arranged evenly around the rotating disk center of circle; One end of described baffle arrangement is connected with the sidewall of central circular boss dish, and axis is on the radius of rotating disk, and the other end connects the edge of rotating disk.
Described baffle arrangement is made of the oblique lower plate that is connected in central circular boss dish sidewall and the T template that is connected with oblique lower plate, be provided with right cylinder between described oblique lower plate and the T template, described cylindrical center line is vertical with the bottom surface in reagent storehouse, with the axis normal of oblique lower plate, with the axis normal of T template.
Described right cylinder is hollow circular tube.
Reagent cup in the described reagent storehouse is set up and is equipped with the through hole that holds reagent cup, two opposite flanks of described reagent glass stand be provided with the reagent storehouse in the circular section groove that mates mutually of right cylinder outer wall.
The relative both sides of the openend of described reaction tube are provided with two fixed blocks that are used for the nick shaped of reaction tube suspension strut, and described fixed block is formed to a side extended 4 " faces " encirclement by the openend outer wall of reaction tube; Described 4 " faces " surround the inner chamber of the fixed block that forms and the inner chamber of reaction tube is the cavity that runs through one.
In sum, the present invention compared with prior art has following advantage:
(1), the present invention realized from reaction tube load, sample is annotated automatically, reagent is annotated automatically, reactant liquor incubation reaction, reactant liquor cleans automatically, reaction result detects and analyzes immunoreaction process full-automatic operation, full-automatic operation has reduced the influence of human factor to experiment, has improved sensitivity.
(2), consumption material box travelling mechanism of the present invention is lateral transfer portion and the vertical broken line shape of delivery section, the operation stroke of consumption material box is longer, once can go up four consumption material boxes, the automaticity height.
(3), in the reagent of the present invention storehouse, rotating disk is provided with right cylinder, reagent glass stand surface is provided with the groove that is complementary, this matching mechanism can effectively reduce the shake of kit, makes being positioned in the reagent storehouse that kit can be more firm.
(4) each parts design of the present invention is ingenious, is convenient to the system integration by cooperatively interacting and realizes robotization, can and can also form the equipment of more automation functions on this invention basis.
Description of drawings
Fig. 1 is the basic composition synoptic diagram of instrument of the present invention.
Fig. 2 is the front section view of instrument of the present invention.
Fig. 3 and Fig. 4 are the structural representation of operative body of the present invention.
Fig. 5 is the partial schematic diagram of the specimen holder of instrument of the present invention.
Fig. 6 is the structural representation of the reagent glass stand of kit of the present invention.
Fig. 7 is the vertical view in reagent of the present invention storehouse.
Fig. 8 is the structural representation in reagent of the present invention storehouse.
Fig. 9 is the partial structurtes synoptic diagram that the present invention is placed with the reagent storehouse of kit.
Figure 10 is the structural representation of the reagent cup of kit of the present invention.
Figure 11 is the structural representation of reagent cup evenly mixing device of the present invention.
Figure 12 and Figure 13 are the structural representations of the reaction tube of instrument of the present invention.
Figure 14 is the structural representation of the reaction tube evenly mixing device of instrument of the present invention.
Figure 15 is the structural representation of the pin cleaning device of instrument of the present invention.
Figure 16 is the structural representation of the ejector sleeve device of instrument of the present invention.
Figure 17 is the structural representation of the cleaning station of instrument of the present invention.
Figure 18 is the structural representation of the detecting unit of instrument of the present invention.
Figure 19 is the structural representation that the sampling of instrument of the present invention moves the pipe arm support.
Figure 20 and Figure 21 are the structural representations of consumption material box of the carrying reaction tube/feed head of instrument of the present invention.
Figure 22 is the structural representation that the sampling of instrument of the present invention moves the pipe arm.
Figure 23 is that instrument sampling of the present invention moves the structural representation that the pipe arm obtains feed head.
Figure 24 is that instrument sampling of the present invention moves the structural representation that the pipe arm obtains reaction tube.
Figure 25 is the structural representation that instrument sampling of the present invention moves the unloading feed head of pipe arm.
Figure 26 is the structural representation of consumption material box conveyer of the present invention.
Figure 27 is the structural representation that the present invention is loaded with the consumption material box conveyer of consumption material box.
Figure 28 is the workflow diagram of full-automatic chemical luminescence immunoassay instrument of the present invention.
The title of each parts correspondence among the figure: 1-operative body, 11-sample bin, 111-specimen holder, the 112-sample hose, the 13-first reagent arm unit, the 15-second reagent arm unit, the pin cleaning device of the 151-second reagent arm, the automatic sample adding device of the 152-second reagent arm, 16-second cleaning station, 18-consumption material box conveyer, 191-substrate filling apparatus, the 192-waste liquid reclaims the unit, 20-controls computing machine, 2-sampling/reaction tube apparatus for automatically loading, 21-reaction tube, the 22-sampling moves the pipe arm, 221-reaction tube stator, 222-feed head stator, 223-unloading feed head device, the 23-consumption material box, the horizontal shifting sledge of 24-, 25-moves radially slide rail, the vertical shifting sledge of 26-, the 27-feed head, 30-storing storehouse, 3-reagent storehouse, 31-reagent cup evenly mixing device, the 311-electric rotating machine, 312-mixing, 313-vortex mixer telecontrol equipment, 32-washing lotion bucket and waste liquid barrel, the 320-reagent cup, 321-reagent glass stand, 33-reaction tube recycling bin, 34-feed head recycling bin, the 40-control box, the low cylinder of 4-reaction warehouse, the pin cleaning device of the 41-first reagent arm, 411-is formed washes the pin district, 42-reaction tube evenly mixing device, the 421-electric rotating machine, 424-reaction tube support, 43-ejector sleeve device, 44-first cleaning station, the 441-adsorbent equipment, 442-liquid sucking device, 443-priming device, the 45-detecting unit, 451-trustship structure, 452-shutter, 453-detection means, 454-reaction tube suspension, the 455-detecting unit transport reactor tube apparatus, 51-round boss dish, 52-baffle arrangement, the oblique lower plate of 53-, the 54-T template, 55-right cylinder, 61-lateral transfer portion, the vertical delivery section of 62-, the 63-cross-shaped portion, 64-consumption material box pushing block, 65-transmission block, the 66-swash plate, the 67-rectangle frame.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
The explanation of nouns of relevant each parts of the present invention:
1) sample hose 111, standard 13 * 75mm sample hose or sample cup.
2) specimen holder 112, and compatibility standard 13 * 75mm sample hose or sample cup, each specimen holder can load 6 sample hoses, and specimen holder can load separately.
3) sample bin 11, are used for the rotating disk of carrying specimen holder, can load 10 specimen holders.
4) sampling moves pipe arm 22, being arranged on sampling and moving on the pipe arm support automatic application of sample and the loader mechanism of integrated automatic application of sample function and reaction tube load function.
5) control box 40, system electronic control and driver element.
6) unloading feed head device 223 removes the device of discarding feed head.Usually a circular hole is set near the consumption material box travelling mechanism, and this circular hole is communicated with feed head recycling bin 34.As shown in figure 25, instrument sampling of the present invention moves the structural representation of a kind of embodiment of unloading feed head of pipe arm.When after feed head uses, needing to abandon, the sampling that has feed head moves pipe arm 22 and runs to unloading feed head device 223 tops, after partly being inserted into feed head in the circular hole that unloads feed head device 223, sampling moves pipe arm 22 and continues operation, feed head is tilted a little, can so that feed head automatically from sampling move the pipe arm come off, drop into feed head recycling bin 34 from circular hole.
7) the reaction tube evenly mixing device 42, as shown in figure 14, comprise locator for fixation reaction pipe upper end, be located at reaction warehouse reaction tube support below mixing, and control mixing to away from or the contact reaction tube side to mobile motion control mechanism, described mixing is connected with the mixing mechanism that is provided with electric rotating machine 421 by eccentric structure; Therefore can be by the carry out mixing of vortex mode to the sample in the reaction tube and reagent.
8) the reagent cup evenly mixing device 31, be located at inner rotary table below, reagent storehouse, as shown in figure 11, comprise electric rotating machine 311, be fixed with mixing in described electric rotating machine 311 rotating shafts, and described evenly mixing device also be connected with control mixing near or away from the vortex mixer telecontrol equipment 313 of reagent cup.The center at described mixing 312 tops is provided with the projection piece, and periphery is provided with the card post, and the quantity of card post is 3; The described projection piece of present embodiment concrete structure can be arranged to the solid that the frustum of a cone and right cylinder are formed by stacking.As shown in figure 10, reagent cup bottom arranges the recess of cylinder barrel shaped, and the catch that constitutes of the radial spoke that is extended outward by recess.The size of the recess of described cylinder barrel shaped is set to the projection piece to be contained in wherein just.When reagent cup needs mixing, mixing with projection piece can rise to the reagent cup bottom and contact, between the projection piece and the recess bottom the reagent cup by mixing, and the coordinated between card post and the catch, can drive the coaxial rotation of reagent cup during the rotation of mixing, thereby make material mixing in the reagent cup.
9) the pin cleaning device 41 of the first reagent arm cleans the reagent needles of the first reagent arm.
10) the first reagent arm unit 13, but the mechanism of vertical movement and the gyration of first reagent of annotating.
11) the ejector sleeve device 43, set according to single stage method and two-step approach, and whether the control response pipe enters first cleaning station 44.As shown in figure 16, the reaction tube support of reaction warehouse is the annulus toothing of going out, and the length of the outer sawtooth of described annulus can be held and hangs two reaction tubes.Whether ejector sleeve device 43 pushes the internal layer of reaction tube support with reaction tube by handling, and whether enters first cleaning station thereby control reaction tube.
12) first cleaning station 44, the integrated attached cleaning unit of magnetic of inhaling waste liquid structure, magnetic bead adsorption structure, filling cleaning fluid structure and mixing structure is used for the cleaning process of the two-step approach first step.As shown in figure 17, be the structural representation of cleaning station.
13) the second reagent arm unit 15, but the mechanism of vertical movement and the gyration of second reagent of annotating.
14) the pin cleaning device 151 of the second reagent arm cleans the reagent needles of the second reagent arm.
15) the second cleaning station unit 16, the integrated attached cleaning unit of magnetic of inhaling waste liquid structure, magnetic bead adsorption structure, filling cleaning fluid structure and mixing structure.
16) the substrate filling apparatus 191, are used for filling luminescence-producing reaction substrate.Substrate filling apparatus 191 is located on the circumference of reaction warehouse 4, behind the reaction tube process cleaning station, before the arrival detecting unit 45, to reaction tube 21 interior filling substrates.This substrate filling apparatus 191 is made up of pump and pipeline.
17) detecting unit 45, and the reaction tube of the luminous substrate of having annotated is carried out luminous detection.
18) waste liquid reclaims unit 192, reclaims the reactant liquor that detects in the reaction tube that finishes.
19) consumption material box 23, are used for the consumptive material support of carrying reaction tube and feed head.
20) feed head 27, standard 300uL feed head.
21) reaction tube 21, are used for the transparent carrier of the potpourri of carrying sample and reagent.The relative both sides of the openend of reaction tube of the present invention are provided with two fixed blocks that are used for the nick shaped of reaction tube suspension strut, and described fixed block is formed to a side extended 4 " faces " encirclement by the openend outer wall of reaction tube; Described 4 " faces " surround the inner chamber of the fixed block that forms and the inner chamber of reaction tube is the cavity that runs through one.Arranging of two fixed blocks makes things convenient for reaction tube can be suspended on the reaction tube support of reaction warehouse.As shown in figure 13, do not have wall to cut off between each fixed block and the reaction tube and constitute the cavity that communicates.The benefit of the setting of this structure is: when needs pipetted reaction tube, sampling moved the pipe arm and need be inserted into the reaction tube stator that making sampling move the pipe arm in the reaction tube and closely contact with the inwall that reacts the mouth of pipe, then reaction tube is mentioned.Because fixed block is nick shaped, when sampling moves the moment that the pipe arm inserts reaction tube, gas can be discharged from the both sides breach in the reaction tube, therefore having reduced sampling and having moved the resistance that the pipe arm inserts reaction tube, makes that to move pipe more smooth; Also reduce simultaneously the wearing and tearing of reaction tube stator, can prolong its serviceable life.
As shown in Figure 1, instrument of the present invention mainly is made up of operative body 1, the control computing machine 20 that is connected with operative body, is provided with storing storehouse 30 and control box 40 below operative body.
As shown in Figure 2, cleaning fluid and waste liquid barrel 32, reaction tube recycling bin 33, feed head recycling bin 34 are set in the storing storehouse 30.Control box 40 inside can arrange refrigeration unit 50 and power supply box 60.Described control computing machine 20 can according to circumstances dispose touching display screen or regular display.
As shown in Figure 3 and Figure 4, be the structural representation of operative body 1.Described operative body 1 comprises following ingredient: sample bin 11, reagent storehouse 3, reaction warehouse 4, sampling/reaction tube apparatus for automatically loading 2.
Reagent storehouse 3, described reagent storehouse comprises the kit that reagent glass stand and reagent cup constitute and the rotating disk that carries kit, and is provided with the temperature control mechanism that the kit activation temperature is provided and the reagent cup evenly mixing device 31 that is used for reagent in the mixing reagent cup below described rotating disk.Rotating disk can rotate around the axle center, and kit is along the rotating disk circumference uniform distribution, and temperature control mechanism is positioned at the kit bottom, can carry out stored refrigerated to reagent.Can place 3 reagent cup in the reagent glass stand, reagent cup bottom has the structure that cooperates with the reagent cup evenly mixing device, this structure to cooperate with reagent cup evenly mixing device 31 to finish the magnetic bead mixing.
Shown in Fig. 7,8 and 9, the rotating disk in the described reagent storehouse 3 comprises central circular boss dish 51 and even some baffle arrangements 52 arranged evenly around the rotating disk center of circle; Adjacent two baffle arrangements 52 constitute a bracket.Generally kit is placed in the bracket, gets final product.One end of described baffle arrangement is connected with the sidewall of central circular boss dish, and axis is on the radius of rotating disk, and the other end connects the edge of rotating disk.Described baffle arrangement 52 is made of the oblique lower plate 53 that is connected in central circular boss dish sidewall and the T template 54 that is connected with oblique lower plate, be provided with the right cylinder 55 that hollow circular tube constitutes between described oblique lower plate 53 and the T template 54, described cylindrical center line is vertical with the bottom surface in reagent storehouse, with the axis normal of oblique lower plate, and the axis normal of T template.
As shown in Figure 6, the reagent cup in the described reagent storehouse is set up and is equipped with the through hole that holds reagent cup, two opposite flanks of described reagent glass stand be provided with the reagent storehouse in the circular section groove that mates mutually of right cylinder outer wall.
The reagent storehouse in use, the reagent glass stand that we will be equipped with reagent cup is plugged between adjacent two baffle arrangements, because the sidewall setting of reagent glass stand and the circular section groove that the right cylinder outer wall in the reagent storehouse mates mutually.Therefore, circular section groove and the right cylinder of reagent glass stand can be fitted mutually, closely contact, and the design of this right cylinder and circular section groove can effectively reduce the shake of reagent glass stand.
The below, position of placing the reagent glass stand between two baffle arrangements of reagent storehouse inner rotary table also arranges through hole, buries the temperature control mechanism that the kit activation temperature is provided and the reagent cup evenly mixing device 31 that is used for reagent in the mixing reagent cup in the through hole underground.As shown in figure 11, described reagent cup evenly mixing device comprises electric rotating machine 311, is fixed with mixing on the output shaft of described electric rotating machine; And described reagent cup evenly mixing device connect control mixing near or away from the vortex mixer telecontrol equipment 313 of reagent cup; Described mixing subcenter is provided with the projection piece, and periphery is provided with 3 card posts; Described projection piece concrete structure can be arranged to the solid that the frustum of a cone and right cylinder are formed by stacking.As shown in figure 10, the bottom of cup of described reagent cup is provided with the recess of the cylindrical drum shape of mating with described projection piece, and the recess of described cylindrical drum shape extends radial spoke laterally and constitutes catch.The size of the recess of described cylinder barrel shaped is set to the projection piece to be contained in wherein just.When reagent cup needs mixing, mixing with projection piece can rise to the reagent cup bottom and contact, between the projection piece and the recess bottom the reagent cup by mixing, and the coordinated between card post and the catch, evenly mixing device is risen touch and to block automatically after the reagent cup and on reagent cup, and drive the reagent cup rotation.Can drive the coaxial rotation of reagent cup when being the rotation of mixing, thereby make material mixing in the reagent cup.Moving up and down by connected vortex mixer telecontrol equipment 313 of mixing 312 parts drives.
Reaction warehouse 4, described reaction warehouse comprises the go out reaction tube support 424 of toothing of annulus, reaction tube support annulus outer radius be provided with filling reagent reagent arm unit 13 and 15, be used for reaction tube is reclaimed unit 192 and reaction tube drop device from ejector sleeve device 43, cleaning station 16 and 44, substrate filling apparatus 191, detecting unit 45 and the waste liquid that the reaction tube support outside is pushed into reaction tube support inboard, be provided with reaction tube evenly mixing device 42 in reaction tube support bottom.As shown in figure 16, the go out reaction tube support of toothing of circle, the tooth of going out is distributed in reaction pipe support periphery, and reaction tube can be placed in by the fixed block of sidewall on the reaction tube support.The reaction tube support tooth depth of going out can be held at least two reaction tubes, and this design makes the present invention applicable to single stage method and two-step approach reaction.The difference of single stage method and two-step approach be single stage method add reagent after temperature bathe once, detect through cleaning laggard detecting unit, two-step approach adds reagent temperature bathes and can add reagent again after cleaning and carry out temperature bath, advances the detecting unit detection then.In order to provide in the reaction tube incubation of material temperature required, temperature control mechanism can be set below reaction warehouse reaction tubes support constitute temperature and bathe the storehouse.In order to satisfy the needs of two kinds of different temperatures, first temperature is set bathes storehouse and the second temperature bath storehouse, totally two temperature are bathed the storehouse.
Described reagent arm unit comprises automatic sample adding device and pin cleaning device.Described automatic sample adding device is made up of reagent needles, punch block, syringe pump and motion, and described reagent needles is installed in by punch block can be up and down or on the motion that swings; Described reagent needles connects T-valve, and described T-valve one end connection expresses pump, and the other end connects syringe pump; And the described pump that expresses is connected with the washing lotion bucket that carries cleaning fluid.The motion of described reagent arm unit is made up of two motors and motion auxiliary, wherein an Electric Machine Control reagent arm unit moves up and down, another Electric Machine Control reagent arm unit swings along axis direction, and the intersection point of reagent arm unit swing circumference and reagent storehouse 3 and reaction warehouse 4 is the sampling position in reagent storehouse and the injection position of reaction warehouse.Therefore, the major function of reagent arm unit is, extracts reagent from the reagent storehouse, is expelled in the reaction tube of reaction warehouse.Reagent needles is convertible closure in T-valve, when reagent needles is connected with syringe pump, can finish absorption and the discharging of reagent by the control of syringe pump; When reagent needles with express pump when being connected, can discharge cleaning fluid fast.
Shown in Figure 14 is the reaction tube evenly mixing device of present embodiment, described reaction tube evenly mixing device comprise fixation reaction pipe upper end locator, be positioned at the reaction tube below mixing, and control mixing to away from or the contact reaction tube side to mobile motion control mechanism, described mixing is connected with the mixing mechanism that is provided with electric rotating machine 421 by eccentric structure.Mixing is connected with the mixing mechanism that is provided with electric rotating machine 421 by eccentric structure, therefore by its eccentric rotation, makes the material mixing under vorticity in the reaction tube that is fixed on the stator.When the reaction tube evenly mixing device is not worked, above-mentioned evenly mixing device external member is positioned at the reaction tube 21 times that hangs over the reaction tube support, with reaction tube 21 segment distance is arranged, in the time of need carrying out the mixing operation to reaction tube 21, mixing external member integral body under another motor of reaction tube evenly mixing device promotes moves upward, and the sub-driving a reaction pipe 21 of mixing is close on stator up to its top.Reaction tube evenly mixing device 42 is positioned at the fixed position on the reaction warehouse disk, is used for adding the system mixing behind the reagent.
Be the ejector sleeve device 43 of present embodiment as shown in figure 16, ejector sleeve device 43 is positioned at reaction warehouse 4 periphery, and near reaction tube support 424, its position is used for distinguishing single stage method and two-step approach reaction before first cleaning station 44.The ejector sleeve device is made up of a motor or electromagnet and motion auxiliary, the reaction tube 21 that is hung on the reaction tube support outside can be pushed into reaction pipe support inboard during work.When using single stage method, do not need to carry out twice application of sample and clean, need to use the ejector sleeve device that reaction tube 21 is pushed into reaction pipe support inboard, avoid cleaning station 44; When using two-step approach, then ejector sleeve device 43 is not worked, and reaction tube 21 is once by two attached cleaning stations of magnetic.
Be the cleaning station of present embodiment as shown in figure 17,2 cleaning stations be set, can be compatible the experiment of two-step approach reaction.Present embodiment cleaning station 16 and 44 comprises supporting construction, respectively arrange on the described supporting construction 3 groups of priming devices 443 and, 4 groups of liquid sucking devices 442; The below of described priming device 443 arranges the reaction tube evenly mixing device 42 with the reaction tube coupling; Install on the sidewall of described supporting construction can the adsorption reaction pipe in the adsorbent equipment 441 of magnetic bead; And described priming device is connected the pump of control liquid absorption and rate of liquid aspiration with liquid sucking device.Reaction tube evenly mixing device 42 can be finished the mixing of liquid by the work in combination of two motors.When reaction tube 21 passes through first cleaning station 44, draw waste reaction solution through liquid sucking device earlier and be discharged into waste liquid and reclaim in the unit 192, add cleaning fluid by priming device 443 again, reaction tube evenly mixing device 42 is with system vortex mixing afterwards, and then enter next liquid sucking device and draw and clean waste liquid, priming device 443 is discharged in the reaction tube 21, and reaction tube evenly mixing device 42 is with the cleaning operation of system vortex mixing, and so repeatedly cleaning operation is carried out in circulation.In above-mentioned cleaning operation process, magnet all wraps in to reaction tube at sidewall and is adsorbed by the magnetic bead of specific probe.Substrate filling apparatus 191 is positioned on reaction warehouse 4 circumference, and reaction tube cleaned back, arrival detecting unit 45 through cleaning station before, substrate filling apparatus 191 was to reaction tube 21 interior filling substrates.This substrate filling apparatus is made up of pump and pipeline.
Shown in Fig. 4 and 18, detecting unit 45 is positioned at outside reaction warehouse 4 circumference, and detecting unit 45 comprises and transports reactor tube apparatus 455, shutter 452, reaction tube suspension 454, detection means 453.Transport reactor tube apparatus 455 and can in the xz plane, do two dimensional motion, it comprise two motors, with trustship structure 451 and the corresponding auxiliary of reaction tube coupling.Deposit mechanism is a fluted body structure, and its groove inwall size wants big than reaction tube 21 outer walls, and reaction tube 21 bottoms are had in the insertion groove of tolerance.Deposit mechanism is connected with motor by auxiliary, can move in the xz plane under the motor effect.Transport reactor tube apparatus 455 during detection and move to reaction tube to be detected earlier 21 times, motor effect subiculum react the pipe 21, make it to leave the reaction tube support, transporting reactor tube apparatus 455 afterwards moves along the x axle, after being positioned on the reaction tube suspension 454 that detects the storehouse to reaction tube 21, transport reactor tube apparatus 455 and move downward, reaction tube 21 sidewall projections are hung over detect on the storehouse reaction tube suspension 454.Shutter 452 is made up of a motor and motion auxiliary, can move along the y axle, seals.After reaction tube 21 hung on the detection storehouse reaction tube suspension 454, shutter 452 was closed rapidly, reaction tube 21 is placed in the confined space of shutter 452 and detection means 453 among the embodiment.Detection means 453 start detection will detect data and feed back to the control computing machine by data line this moment.Detection finishes, shutter 452 is opened, reaction tube 21 returns to the reaction tube support by transporting reactor tube apparatus 455, rotation along with the reaction tube support, be hung on the reaction tube 21 of reaction tube support after after testing and be sent to the reaction tube drop device by the gathering sill that is positioned at reaction warehouse 4 bottoms, leave reaction warehouse to reaction tube 21, the experiment testing of single sample is finished.
Sampling/reaction tube apparatus for automatically loading 2, described sampling/reaction tube apparatus for automatically loading comprise the consumption material box conveyer 18 that transports consumption material box 23 that the sampling of being located between sample bin and the reaction warehouse moves pipe arm 22, sampling and move pipe arm below, and unloading feed head device 223; Described consumption material box is provided with feed head 27 and reaction tube 21, and described sampling moves pipe arm lower end and is provided with feed head stator 222 and the reaction tube stator 221 that is made of the step O-ring seal.Described consumption material box conveyer comprises lateral transfer portion 61 and vertical delivery section 62, and described lateral transfer portion and vertical delivery section intersect vertically and constitute cross-shaped portion 63, and described cross-shaped portion is provided with the inductor for induction consumption material box position.The lateral transfer portion of described consumption material box conveyer is along the edge line setting of operative body; Comprise feed belt, be used for holding up the carrying roller of feed belt and the PTO that is connected with feed belt.Vertical delivery section of described consumption material box conveyer is located between sample bin and the reaction warehouse, is positioned at the vertical lower that sampling moves the pipe arm.Vertical delivery section of described consumption material box conveyer comprises guiding thick stick, leading screw and transmission block, one end of the upper surface of described transmission block convexes to form consumption material box pushing block 64, described transmission block 65 is provided with two parallel screws, and described guiding thick stick and leading screw pass from described screw respectively.During concrete enforcement, also be provided with swash plate 66 at the vertical end of delivery section 62, swash plate connects rectangle frame 67, and described rectangle frame 67 is communicated with the consumption material box recycling bin.Before the experiment beginning, the user is positioned over consumption material box 23 on the feed belt of lateral transfer portion 61, and the feed belt by lateral transfer portion is sent to cross-shaped portion 63, the position that this moment, inductor can be sensed consumption material box; So vertically the consumption material box pushing block of delivery section 62 is activated, under the drive of leading screw and transmission block, promote consumption material box 23 and move along the guiding thick sticks, consumption material box 23 is delivered to sampling move pipe arm 22 belows and stop.As shown in figure 19, sampling moves pipe arm 22 and is arranged on the sampling of being made up of horizontal shifting sledge 24 and vertical shifting sledge 26 and moves and manage on the arm support.Sampling moves pipe arm 22 and is fixed on the vertical shifting sledge 26, and vertical shifting sledge 26 is fixed on the horizontal shifting sledge 24, takes a sample by the motion realization of two guide rails and moves the motion of pipe arm 22 in two-dimentional vertical plane.After the reaction tube on the consumption material box and feed head ran out of, consumption material box pushing block 64 started consumption material box by swash plate 66, and sent into the consumption material box recycling bin through rectangle frame 67.
As Figure 20 and shown in Figure 21 be the consumption material box that present embodiment uses, described consumption material box can hold feed head and reaction tube simultaneously; In the present embodiment consumption material box half hold feed head 27, half holds reaction tube 21; Be provided with 10 row positions, hole as consumption material box, 5 classify the reaction pore position of loading reaction tube 21 as, 5 classify the position, feed head hole of loading feed head as in addition.
As Figure 12 and shown in Figure 13 be the reaction tube 21 that present embodiment is suitable for, this reaction tube inside is circular, outer wall is square, the relative both sides of the openend of described reaction tube are provided with two fixed blocks that are used for the nick shaped of reaction tube suspension strut, and described fixed block is formed to a side extended 4 " faces " encirclement by the openend outer wall of reaction tube; Described 4 " faces " surround the inner chamber of the fixed block that forms and the inner chamber of reaction tube is the cavity that runs through one.Arranging of fixed block can be fixed on reaction tube on the reaction tube support of reaction warehouse, perhaps on the reaction tube suspension of detecting unit.In addition, because the inner chamber of fixed block and reaction tube inner chamber connect, when making sampling move the pipe arm to pipette reaction tube, resistance is less, makes the reaction tube stator more successfully embed the mouth of pipe that fits to reaction tube.
The shown in Figure 22 sampling for present embodiment moved pipe arm 22, it comprises motor, piston, its end is provided with by two-stage reaction tube stator 221 and the feed head stator 222 that the step of O-ring seal constitutes is housed, and wherein step feed head stator 222 diameters near terminal are littler than reaction tube stator.
Shown in Figure 23,24 and 25, move the working state figure of pipe arm for the present invention's sampling.As shown in figure 23, be inserted in the synoptic diagram that feed head carries out application of sample or sampling for feed head stator 222.As shown in figure 24, reaction tube stator 221 is inserted in the synoptic diagram that reaction tube 21 is prepared the mobile response pipe.As shown in figure 25, move the pipe arm unloads feed head in unloading feed head device 223 work synoptic diagram for sampling.
The reaction pattern that is suitable for according to the invention instrument in the enforcement is determined the quantity of reagent arm unit, reagent arm pin cleaning device and cleaning station.The present invention is in real work, and the detection of each sample is that serial is carried out successively, detects flux for improving, and above-mentioned each mechanism is according to detecting index, walking abreast according to the working routine of setting and carry out work all the time in process of the test.As shown in figure 28, be flow process chart of the present invention.
The quantity of present embodiment reagent arm unit is two: the first reagent arm unit 13 and the second reagent arm unit 15; Corresponding pin cleaning device is: the pin cleaning device 151 of the pin cleaning device 41 of the first reagent arm and the second reagent arm.Cleaning station quantity is two: first cleaning station 44 and second cleaning station 16.
The operational scheme of present embodiment is: as shown in figure 27, on the feed belt of the consumption material box conveyer lateral transfer device 91 of before experiment described consumption material box being packed into, can place a plurality of consumption material boxes simultaneously according to the experiment situation.The consumption material box of carrying reaction tube/feed head is along mobile under the feed belt effect of lateral transfer device 91, when consumption material box is transported to cross-shaped portion 63, vertically the consumption material box pushing block 64 of delivery section 62 starts, under the leading screw effect, consumption material box is transported along the guiding thick stick to vertical delivery section, move under pipe arm 22 planes in sampling up to consumption material box first ranking.As shown in figure 19, sampling afterwards moves pipe arm 22 slides and laterally moves to the feed head position under the shifting sledge effect vertical movement that sampling moves the pipe arm support, up to being positioned at directly over the target feed head, embed to the target feed head under vertical shifting sledge effect then, the step feed head stator 222 that moves the pipe arm up to sampling is tightly connected with feed head.Sampling afterwards moves the pipe arm in the sampling position that moves to sample bin 11, being moved downward to feed head submerges in the sample, sampling moves the counter-rotating of pipe arm 22 motors then, draw sample by feed head, after inhaling sample and finishing, sampling moves the pipe arm and moves on the consumption material box directly over the goal response pipe 21, is moved down into feed head and enters in the reaction tube 21, sampling moves pipe arm 22 motors then just changes, and discharges sample by feed head.Sampling behind the stock layout moves the pipe arm and moves to unloading feed head device 223, and sampling moves the pipe arm and moves to side slightly, and feed head namely can be discharged in the unloading feed head device 223.Sampling after the sampling moves the pipe arm and moves on on the reaction tube 21 after the load sample, inserts downwards in the reaction tube 21, and the O-ring seal by step reaction tube fixed word 221 closely connects.Sampling afterwards moves the pipe position of putting that the pipe arm moves to reaction warehouse 4, and reaction tube 21 unloadings are hung on the reaction tube support, and the simple sample pipe pipe process of taking a sample/move finishes.When needs carried out the multitube application of sample and move pipe, after front-seat reaction tube was exhausted, consumption material box was mobile under vertical delivery section effect of consumption material box travelling mechanism, was positioned at sampling up to new row's reaction tube and moved pipe arm plane of movement.After the feed head on the consumption material box and reaction tube used up, consumption material box pushing block 64 started consumption material box by swash plate 66, and sent into the consumption material box recycling bin through rectangle frame 67.Simultaneously, next consumption material box is transported by lateral transfer portion, starts the next round circulation.
After the reaction tube that has carried sample is suspended on the reaction warehouse, filling first reagent in the reaction tube of the first reagent arm unit 13 in the reaction warehouse.If this moment program setting be single stage method, then directly by the reaction tube evenly mixing device with material mixing in the reaction tube; If program setting is two step method, then earlier through the second reagent arm unit 15 filling second reagent in the reaction tube, again by the reaction tube evenly mixing device with material mixing in the reaction tube.Reaction tube behind the mixing enters the first temperature bath storehouse of reaction warehouse and carries out the first temperature bath reaction.
After incubation finished, if program setting is single stage method, reaction tube directly was admitted to the second temperature bath storehouse and carries out the second temperature bath reaction.If program setting is two-step approach, the reaction tube that first temperature is bathed after reaction finishes is admitted to first cleaning station earlier, after first cleaning station cleans three times, annotate second reagent to reaction tube by the second reagent arm unit, send into the second temperature bath storehouse again and carry out the second temperature bath reaction.
Second temperature is bathed after reaction finishes, and sends into second cleaning station and cleans, clean finish after, by substrate filling apparatus 191 filling luminescence-producing reaction substrate in the reaction tube.Then by the reaction tube evenly mixing device with material mixing in the reaction tube, behind the mixing, reaction tube is sent into detecting unit detects.After detecting end, reaction tube is directed to the reaction tube drop device; So far, the detection of a reaction tube finishes.
As mentioned above, just can realize the present invention preferably.
Claims (5)
1. full-automatic chemical luminescence immunoassay instrument comprises operative body, and connected control computing machine, is provided with control box and storing storehouse below operative body, it is characterized in that described operative body comprises:
Sample bin (11), described sample bin comprise by motor-driven controlled rotating circular disk and the bar code scanner of being located at the controlled rotating circular disk outside, and described bar code scanner is connected with the control computing machine by data line;
Reagent storehouse (3), described reagent storehouse comprises the kit that reagent glass stand and reagent cup constitute and the rotating disk that carries kit, and is provided with the temperature control mechanism that the kit activation temperature is provided and the reagent cup evenly mixing device (31) that is used for reagent in the mixing reagent cup below described rotating disk; Described reagent cup evenly mixing device comprises electric rotating machine (311), is fixed with mixing on the output shaft of described electric rotating machine; And described reagent cup evenly mixing device connect control mixing near or away from the vortex mixer telecontrol equipment (313) of reagent cup; Described mixing subcenter is provided with the projection piece, and periphery is provided with the card post; The bottom of cup of described reagent cup is provided with the recess of the cylindrical drum shape of mating with described projection piece, and the recess of described cylindrical drum shape extends radial spoke laterally and constitutes catch;
Reaction warehouse (4), described reaction warehouse comprises the go out reaction tube support (424) of toothing of annulus, be provided with the reagent arm unit (13 of filling reagent in reaction tube support annulus outer radius, 15), be used for reaction tube is pushed into from the reaction tube support outside ejector sleeve device (43), the cleaning station (16 of reaction tube support inboard, 44), substrate filling apparatus (191), detecting unit (45) and waste liquid reclaim unit (192), is provided with reaction tube evenly mixing device (42) in reaction tube support bottom; Described reagent arm unit comprises automatic sample adding device and pin cleaning device, and described automatic sample adding device is made up of reagent needles, punch block, syringe pump and motion, and described reagent needles is installed in by punch block can be up and down or on the motion that swings; Described reagent needles connects T-valve, and described T-valve one end connection expresses pump, and the other end connects syringe pump; And the described pump that expresses is connected with the washing lotion bucket that carries cleaning fluid; Low cylinder, two concentric drums of outer high cylinder and connected waste liquid barrel in described pin cleaning device comprises, the low cylinder bottom of described inside be solid construction, is hollow-core construction bottom the high cylinder of described outside and is communicated with waste liquid barrel by pipeline; Described cleaning station comprises supporting construction, and priming device (443) and liquid sucking device (442) respectively are set on the described supporting construction; The below of described priming device (443) arranges the reaction tube evenly mixing device (42) with the reaction tube coupling; Install on the sidewall of described supporting construction can the adsorption reaction pipe in the adsorbent equipment (441) of magnetic bead; And described priming device is connected the pump of control liquid absorption and rate of liquid aspiration with liquid sucking device; Described detecting unit (45) comprises shutter (452), reaction tube suspension (454), detection means (453) and transports reactor tube apparatus (455); The described reactor tube apparatus (455) that transports is provided with the trustship structure (451) of mating with reaction tube; Described reaction tube evenly mixing device (42) comprises locator for fixation reaction pipe upper end, is positioned at mixing of reaction tube below, and control mixing to away from or the contact reaction tube side to mobile motion control mechanism, described mixing is connected with the mixing mechanism that is provided with electric rotating machine by eccentric structure;
Sampling/reaction tube apparatus for automatically loading (2), described sampling/reaction tube apparatus for automatically loading comprise that the sampling of being located between sample bin and the reaction warehouse moves pipe arm (22) and sampling moves the consumption material box conveyer (18) that transports consumption material box (23) of managing the arm below; Described consumption material box is provided with feed head (27) and reaction tube (21), and described sampling moves pipe arm lower end and is provided with feed head stator (222) and the reaction tube stator (221) that is made of the step O-ring seal; Described consumption material box conveyer comprises lateral transfer portion (61) and vertical delivery section (62), and described lateral transfer portion and vertical delivery section intersect vertically and constitute cross-shaped portion (63), and described cross-shaped portion is provided with the inductor for induction consumption material box position;
Rotating disk in the described reagent storehouse (3) comprises central circular boss dish (51) and even some baffle arrangements (52) arranged evenly around the rotating disk center of circle; One end of described baffle arrangement is connected with the sidewall of central circular boss dish, and axis is on the radius of rotating disk, and the other end connects the edge of rotating disk;
Described baffle arrangement is made of the oblique lower plate (53) that is connected in central circular boss dish sidewall and the T template (54) that is connected with oblique lower plate, be provided with right cylinder (55) between described oblique lower plate and the T template, described cylindrical center line is vertical with the bottom surface in reagent storehouse.
2. full-automatic chemical luminescence immunoassay instrument according to claim 1 is characterized in that, described right cylinder (55) is hollow circular tube.
3. full-automatic chemical luminescence immunoassay instrument according to claim 2, it is characterized in that, reagent cup in the described reagent storehouse is set up and is equipped with the through hole that holds reagent cup, two opposite flanks of described reagent glass stand be provided with the reagent storehouse in the circular section groove that mates mutually of right cylinder outer wall.
4. according to each described full-automatic chemical luminescence immunoassay instrument of claim 1 to 3, it is characterized in that, the relative both sides of the openend of described reaction tube are provided with two fixed blocks that are used for the nick shaped of reaction tube suspension strut, and described fixed block is formed to a side extended 4 " faces " encirclement by the openend outer wall of reaction tube; Described 4 " faces " surround the inner chamber of the fixed block that forms and the inner chamber of reaction tube is the cavity that runs through one.
5. full-automatic chemical luminescence immunoassay instrument according to claim 1 is characterized in that, the lateral transfer portion of described consumption material box conveyer is along the edge line setting of operative body; Comprise feed belt, be used for holding up the carrying roller of feed belt and the PTO that is connected with feed belt;
Vertical delivery section of described consumption material box conveyer is located between sample bin and the reaction warehouse, is positioned at the vertical lower that sampling moves the pipe arm; Vertical delivery section of described consumption material box conveyer comprises guiding thick stick, leading screw and transmission block, one end of the upper surface of described transmission block (65) convexes to form consumption material box pushing block (64), described transmission block is provided with two parallel screws, and described guiding thick stick and leading screw pass from described screw respectively.
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| CN 201110243705 CN102419375B (en) | 2011-08-24 | 2011-08-24 | Full-automatic chemoluminescence immunoassay analyzer |
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Application publication date: 20120418 Assignee: SICHUAN MAKER BIOMEDICAL ELECTRONIC CO., LTD. Assignor: Sichuan Maker Biological Science and Technology Co., Ltd. Contract record no.: 2014510000174 Denomination of invention: Full-automatic chemiluminescence immune analyzer Granted publication date: 20130828 License type: Common License Record date: 20141112 |
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Address after: High tech Zone Chengdu city Sichuan province 611731 rivers Road No. 16 Patentee after: Mike biological Limited by Share Ltd Address before: High tech Zone Chengdu city Sichuan province 611731 rivers Road No. 16 Patentee before: Sichuan Maker Biological Science and Technology Co., Ltd. |