CN102379368B - Non-resistance cultivation method with joint application of feed strain, disinfectant bacterium and biological isolation - Google Patents
Non-resistance cultivation method with joint application of feed strain, disinfectant bacterium and biological isolation Download PDFInfo
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- CN102379368B CN102379368B CN 201110293281 CN201110293281A CN102379368B CN 102379368 B CN102379368 B CN 102379368B CN 201110293281 CN201110293281 CN 201110293281 CN 201110293281 A CN201110293281 A CN 201110293281A CN 102379368 B CN102379368 B CN 102379368B
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Abstract
The invention belongs to the field of animal husbandry and specifically relates to a non-resistance cultivation method with joint application of feed strain, disinfectant bacterium and biological isolation. It is characterized in the following three aspects: (1) adding feed strain to feed; (2) spraying disinfectant bacterial suspension to environment; and (3) planting trees and traditional Chinese herbs with disinfectant function on isolation zones among houses. The invention obviously improves the feeding environment, increases the cultivation income, reduces the feed cost and cultivation risk, and provides guarantee to production of high-quality non-resistance products.
Description
Technical field
The invention belongs to the animal husbandry field, be specifically related to the nonreactive cultivation method of a kind of feed, disinfecting bacterium and biological isolation associating.
Background technology
Along with the continuous expansion of breeding scale level and the raising of world agriculture economic globalization degree.China's animal husbandry faces unprecedented development space and huge challenge for existence.At present, there are many problems in China's animal husbandry, and for example: (1) human major product and requirement of byproduct to animal husbandry is more and more higher; (2) epidemic disease is more and more serious to the threat of the health of animal population and breeding environment; (3) the crop products price is constantly soaring, causes animal husbandry pressure increasing; (4) the international trade barrier affects the fast development of China's animal husbandry; (5) the breeding pollution problem is more and more outstanding, becomes the bottleneck of Animal husbandry development, and has caused the great attention of society and government; (6) abuse of antibiotic etc not only to animal, even causes larger hidden danger to health.
The existence of these problems has produced tremendous influence to animal husbandry, so Animal husbandry all begins actively to seek and a kind ofly can improve animal welfare, reduce environmental pollution, can improve again the cultural method of consumption safety.
At present, in the animal husbandry cultivation, add useful microorganism species and become a scientific and reasonable technology, but these bacterial classifications of how arranging in pairs or groups have become the project that each enterprise falls over each other to study.
The EM bacterium is that a kind of Mixed Microbes generally comprises Bacillus, saccharomycete, actinomyces, nitrogen-fixing bacteria, cellulose-decomposing bacterium, lactic acid bacteria etc., cultivates the effectively compound microbial flora that forms through specific process.Can be used for food and add, the farming disease harms control, waste water control etc.
Bacillus licheniformis cellular morphology and arrangement are shaft-like, Dan Sheng, capable of regulating flora (Bifidobacterium Bifidum, Bacillus acidi lactici, bacteroid, peptostreptococcus are had the promotion growth) imbalance reaches therapeutic purposes, can impel body to produce antibacterial substance, kill pathogenic bacteria (pathogenic bacteria such as staphylococcus, yeast-like fungus are had antagonism).Can produce the resistant activity material, and have unique biology and take the oxygen mechanism of action by force, can suppress the growth and breeding of pathogenic bacteria.
Bacillus subtilis is a kind of of bacillus.The subtilin that produces in the bacillus subtilis thalli growth process, polymyxins, nystatin, gramicidins isoreactivity material, these active materials have obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection.Bacillus subtilis consumes rapidly the free oxygen in the environment, causes the enteron aisle hypoxemia, promotes the growth of useful anaerobic bacteria, and produces the organic acid such as lactic acid, reduces the enteron aisle pH value, indirectly suppresses other pathogenic bacteria growth.Thalline self synthesizes the enzymes such as AMS, protease, lipase, cellulase, in alimentary canal with animal body in digestive enzymes together play a role; The multiple B family vitamins such as energy synthetic vitamin B1, B2, B6, nicotinic acid, the activity of interferon and macrophage in the raising animal body.
Summary of the invention
The nonreactive cultivation method that the purpose of this invention is to provide a kind of feed, disinfecting bacterium and biological isolation associating, can carry out each bacterial classification reasonably combined and then be applied in the links of cultivation, and take biological isolation so that whole cultivation is more scientific and reasonable, avoid the epidemic disease and the problem of environmental pollution that occur in the breeding process, improve the quality of animal husbandry major product and byproduct.
The nonreactive cultivation method of a kind of feed of the present invention, disinfecting bacterium and biological isolation associating comprises following three aspects:
(1) according to the standard that adds 2～4kg feed spawn in the feed per ton, in feed, add feed spawn, wherein, the bacterial content of feed spawn is 50～10,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 2～3 parts of bacillus licheniformis and 1 part of bacillus subtilis;
(2) the disinfecting bacterium kind is made the disinfecting bacterium suspension that concentration is 50～10,000,000,000 viable bacterias/mL, then the disinfecting bacterium suspension according to 1: 50～100 quality than thin up, last spray disinfectant in feeding environment, wherein, the disinfecting bacterium kind adopts the EM bacterium;
(3) plantation landscape tree and Chinese herbal medicine with disinfective action replace the isolation strip of adjacent turn my humble abode.
Wherein, the landscape tree in aspect (3) is preferably one or more in Chinese wax, method paulownia or the Chinese scholar tree, and Chinese herbal medicine is preferably honeysuckle.Isolation forms the transfer barrier that daily pathogen is spread as biology for the landscape tree of planting and Chinese herbal medicine, guarantee that after epidemic disease occurs infectious disease popular take air as main route of transmission even do not occur for few generation between letting animals feed, avoid as far as possible even stop cross-infection between the letting animals feed epidemic disease.
The present invention does not add any antibiotic in whole breeding process, reduce aquaculture cost, and avoided the healthy hidden danger of antibiotic to animal and human's body.
The invention has the advantages that: in breeding process, more than three aspect use in conjunction, add scientific and reasonable feeding and management, guaranteeing that letting animals feed obtains under the condition of suitable growth temperature, suppress growing of harmful bacteria in the feeding environment by feed and environment disinfected with the advantageous effect of microorganism, reduce ill probability.The air borne approach of the plantation pathogenic microorganism capable of blocking by landscape tree and Chinese herbal medicine is being avoided importing into and reducing and reduce the cultivation risk aspect the cross-infection of epidemic disease.The present invention has obviously improved feeding environment, has improved the cultivation income, has reduced feeding cost and cultivation risk, provides powerful guarantee for obtaining high-quality nonreactive product.
The specific embodiment
The present invention will be further described below in conjunction with example.
Embodiment 1:
Present embodiment comprises following 3 aspects take feeding of broiler as example:
(1) according to the standard that adds the 2kg feed spawn in the feed per ton, add feed spawn in feed, wherein, the bacterial classification content of feed spawn is 8,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 3 parts of bacillus licheniformis and 1 part of bacillus subtilis;
(2) the disinfecting bacterium kind is mixed with the disinfecting bacterium suspension that concentration is 6,000,000,000 viable bacterias/mL, then the disinfecting bacterium suspension according to 1: 55 quality than thin up, last spray disinfectant in feeding environment, wherein, the disinfecting bacterium kind adopts the EM bacterium;
(3) alternately plant the isolation strip that Chinese wax, method paulownia, Chinese scholar tree, honeysuckle replace the adjacent turn my humble abode.
The indices data that present embodiment obtains are as follows:
(1) general index:
| Project | Breeding cycle | Feedstuff-meat ratio | Deliver average weight for sale | Survival rate | Case fatality rate | Antibiotic uses |
| Numerical value | 42d | 1.66∶1 | 2.9Kg | 95％ | 0 | Nothing |
(2) the residual detection of medicine: (detecting index take the weight of animals as standard)
| Sequence number | Project | Result (μ g/Kg) | Sequence number | Project | Result (μ g/Kg) |
| 1 | Nitroimidazole | Do not detect | 9 | Clenbuterol | 0 |
| 2 | Sulfanilamide (SN) (15) | 0 | 10 | Quinolone (13) | 0 |
| 3 | Tetracycline (4) | Do not detect | 11 | Salbutamol | 0 |
| 4 | Chloramphenicol | 0 | 12 | Ractopamine | 0 |
| 5 | Nitrofurazone | 0 | 13 | ROX | 0 |
| 6 | Furaltadone | 0 | 14 | Tylosin | 0 |
| 7 | Furantoin | 0 | 15 | Lincomycin | 0 |
| 8 | Furazolidone | 0 | 16 | Erythromycin | 0 |
Embodiment 2:
Present embodiment is raised as example take the meat duck, comprises following 3 aspects:
(1) according to the standard that adds the 2kg feed spawn in the feed per ton, add feed spawn in feed, wherein, the bacterial classification content of feed spawn is 7,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 2 parts of bacillus licheniformis and 1 part of bacillus subtilis;
(2) the disinfecting bacterium kind is mixed with the disinfecting bacterium suspension that concentration is 9,000,000,000 viable bacterias/mL, then the disinfecting bacterium suspension according to 1: 90 quality than thin up, last spray disinfectant in feeding environment, wherein, the disinfecting bacterium kind adopts the EM bacterium;
(3) alternately plant the isolation strip that Chinese wax, method paulownia, Chinese scholar tree, honeysuckle replace the adjacent turn my humble abode.
The indices data that present embodiment obtains are as follows:
(1) general index:
| Project | Breeding cycle | Feedstuff-meat ratio | Deliver average weight for sale | Survival rate | Case fatality rate | Antibiotic uses |
| Numerical value | 42d | 1.86∶1 | 3.0Kg | 94.1％ | 0 | Nothing |
(2) the residual detection of medicine: (detecting index take the weight of animals as standard)
| Sequence number | Project | Result (μ g/Kg) | Sequence number | Project | Result (μ g/Kg) |
| 1 | Nitroimidazole | Do not detect | 9 | Clenbuterol | 0 |
| 2 | Sulfanilamide (SN) (15) | 0 | 10 | Quinolone (13) | 0 |
| 3 | Tetracycline (4) | Do not detect | 11 | Salbutamol | 0 |
| 4 | Chloramphenicol | 0 | 12 | Ractopamine | 0 |
| 5 | Nitrofurazone | 0 | 13 | ROX | 0 |
| 6 | Furaltadone | 0 | 14 | Tylosin | 0 |
| 7 | Furantoin | 0 | 15 | Lincomycin | 0 |
| 8 | Furazolidone | 0 | 16 | Erythromycin | 0 |
Claims (3)
1. a feed, disinfecting bacterium and biology are isolated the nonreactive cultivation method of uniting, and it is characterized in that comprising following three aspects:
(1) according to the standard that adds 2～4kg feed spawn in the feed per ton, in feed, add feed spawn, wherein, the bacterial content of feed spawn is 50～10,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 2～3 parts of bacillus licheniformis and 1 part of bacillus subtilis;
(2) the disinfecting bacterium kind is made the disinfecting bacterium suspension that concentration is 50～10,000,000,000 viable bacterias/mL, then the disinfecting bacterium suspension according to the quality of 1:50～100 than thin up, last spray disinfectant in feeding environment, wherein, the disinfecting bacterium kind adopts the EM bacterium;
(3) plantation landscape tree and Chinese herbal medicine with disinfective action replace the isolation strip of adjacent turn my humble abode.
2. the nonreactive cultivation method of feed according to claim 1, disinfecting bacterium and biological isolation associating is characterized in that landscape tree in the aspect (3) is more than one in Chinese wax, method paulownia or the Chinese scholar tree.
3. the nonreactive cultivation method of feed according to claim 1, disinfecting bacterium and biological isolation associating is characterized in that the Chinese herbal medicine in the aspect (3) is honeysuckle.
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| CN 201110293281 CN102379368B (en) | 2011-09-29 | 2011-09-29 | Non-resistance cultivation method with joint application of feed strain, disinfectant bacterium and biological isolation |
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| CN102379368B true CN102379368B (en) | 2013-03-20 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760431A (en) * | 2010-01-05 | 2010-06-30 | 北京市农林科学院 | Compound microbial culture starter and application thereof |
| CN102008016A (en) * | 2010-11-09 | 2011-04-13 | 南京农业大学 | Probiotic solid powder and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760431A (en) * | 2010-01-05 | 2010-06-30 | 北京市农林科学院 | Compound microbial culture starter and application thereof |
| CN102008016A (en) * | 2010-11-09 | 2011-04-13 | 南京农业大学 | Probiotic solid powder and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 圈舍周围绿化的作用;张金平;《河南科技报》;20081216;全文 * |
| 张金平.圈舍周围绿化的作用.《河南科技报》.2008,全文. |
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