CN102344888A - Circulating type photobioreactor - Google Patents
Circulating type photobioreactor Download PDFInfo
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- CN102344888A CN102344888A CN2010102488596A CN201010248859A CN102344888A CN 102344888 A CN102344888 A CN 102344888A CN 2010102488596 A CN2010102488596 A CN 2010102488596A CN 201010248859 A CN201010248859 A CN 201010248859A CN 102344888 A CN102344888 A CN 102344888A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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Abstract
The present invention provides a circulating type photobioreactor. The circulating type photobioreactor comprises a first culture part, a second culture part and a pump part used for connecting the first culture part and the second culture part. The first culture part comprises a culture pot used for supplying a medium and a first light source which irradiates the inner culture pot and is coupled with the culture pot. The second culture part comprises a culture tube arranged outside the culture pot used for supplying the culture from the culture pot and a second light source which irradiates the inner culture tube and is coupled with the culture tube. The pump part is connected with the first culture part and the second culture part for circulating a culture solution between the two parts.
Description
Background of invention
1. invention field
Following specification sheets relates to culture apparatus, particularly circulating bioreactor.
2. the description of association area
At present, the crop of plantation is used as human main food source in the world wide.But the output in every acre of soil is not too high, and the energy efficiency of sun power is extremely low.On the contrary; It is favourable in water, can utilizing the little algae of photosynthesis of sun power, carbonic acid gas and the growth of small amounts of inorganic salt; Because they can be cultivated in the place that crop can not grow; Every acre protein yield is made at least 20 times of object heights than routine, and can be provided at the various useful matteies and the rare crude substance that can not produce in mikrobe, the animal and plant.Specifically, because the alga cells size is bigger, can precipitate well, it is easy therefore separating them and therefrom extracting some materials.Therefore in addition, they, have efficient utilization and use sun power as main energy sources with sun power.In addition and since they with carbonic acid gas as carbon source, have the photosynthesizer that produces oxygen as by product, so they can alleviate air pollution.
Therefore; Comprise the little algae of photosynthesis that Chorella (Chlorella), Dunaliella salina belong to (Dunaliella) and Spirullina (Spirulina) particularly little algae of Spirullina (hereinafter referred to as " tenaculat Habenaria ") become the research hot topic; Because they are bigger than the little algae stature of other photosynthesis; In the alkaline pollution environment, grow easily, can be applicable to food, medicine and other industry.Simultaneously, have, developing indoor cultivation technology rather than outdoor culture technique such as the area, temperate zone (like Korea S) that makes a clear distinction between the four seasons, changes at temperature and sunshine between season climate characteristic greatly.
For example; Korean Patent discloses bactogen No. 235182, and this device comprises auto controlled heater, have the culture vessel of breather, have the rubber hose of the shelf of the equipment that is used to support culture vessel, the lateral substratum supply pipe that is coupled to shelf, the culture soln that is used to circulate and the water pump of the culture soln that is used to circulate.The Korean Patent communique discloses the device that is used for culturing microalgae 2004-0019298 number; This device comprises the culture vessel that is made into the bicylindrical type; It is made up of interior cylinder (horizontal positioned) and outside cylinder; Wherein outside cylinder comprises the transparent material of transmissive light at least, and gas feed is opened on the bottom of culture vessel.Korean Patent discloses the outdoor culture vessel of water channel type that is used for little algae for No. 679989, the wherein integrated inoculation culture container that is used to cultivate inoculum that is equipped with.But if cultivate tenaculat Habenaria with these devices, the tenaculat Habenaria of growth can excessively be attached at the surface of culture vessel.Therefore, the culture efficiency step-down, and because various microbiological contamination can not obtain favourable cultured products.
In order to address the above problem, developed such method, it comprises with the culture vessel sealing with through preventing various microbiological contamination with strainer filtration air supplied.For example, the Korean Patent communique discloses outdoor large scale culturing device for 2002-0057882 number, and this device comprises equipment, pneumatic pump, straining installation, conduit, strainer, needle-valve and the culture vessel that is used to filter with the ultraviolet sterilization; The Korean Patent communique discloses the high-density culture device 2002-0083558 number, and this device is included in shelf, pH unit, pneumatic pump and the CO2 culture tank of the band luminescent lamp of the culture vessel, the pH transmitter in this culture vessel and the divider that have lid on its top, this culture vessel.
Summary of the invention
Although the various devices of using under the prior art possibly prevent various microbiological contamination,, the little algae of photosynthesis cause the problem of culture efficiency decline not to be resolved as yet so far but excessively being attached at the culture vessel internal surface.If problem is resolved, possibly obviously improve the culture efficiency of the little algae of photosynthesis.But, do not instruct the report of this solution.Therefore, thus the purpose of this invention is to provide and can prevent that the little algae of photosynthesis is attached at little algae that the culture vessel internal surface improves culture efficiency and cultivates and use novel culture apparatus.
The present invention attempts to address the above problem and accomplish the present invention; And through confirming when the little algae of photosynthesis is cultivated in a looping fashion, the area that culture soln is exposed to light is maximized and the little algae of photosynthesis that grows can not be attached at the culture vessel internal surface and confirm to accomplish the present invention.
In one aspect, the present invention provides circulating photosynthesis bio-reactor, and this bio-reactor comprises:
First cultivates part, and this first is cultivated part and comprise the culture tank that substratum wherein is supplied and be coupled to culture tank with the first inner light source of irradiation culture tank;
Second cultivates part, and this second is cultivated part and comprise that to be arranged on culture tank outside and by from the culture tube of culture tank supply culture soln be coupled to culture tube with the inner secondary light source of irradiation culture tube; With
The pump part, this pump part and first is cultivated partly and the second cultivation part is connected, so that culture soln is cultivated circulation between the part and second cultivation partly first.
In one embodiment, first light source is arranged on culture tank inside, but is not limited to such setting.In one embodiment, secondary light source is coupled to the internal surface of culture tube or is arranged on the culture tube outside along the culture tube longitudinal direction, but is not limited to such setting.In another embodiment, culture tube preferably has parallel repeatedly folded form, comprises a plurality ofly being coupled to the outside secondary light source of culture tube along the culture tube longitudinal direction, but is not limited to such setting.In a preferred embodiment, the internal diameter of culture tube is 3-30cm.In a preferred embodiment, internal diameter is 5-20cm.In most preferred embodiment, internal diameter is 10-15cm.In a preferred embodiment; First cultivates at least one first culture soln import and at least one first culture soln outlet that part comprises that also both are connected with culture tank and pump part; The second cultivation part also comprises passes through pump part and the outlet of first culture soln and at least one second culture soln import that is connected with the second culture soln import and the outlet of at least one second culture soln respectively, but is not limited to such setting.In another preferred embodiment; First cultivates part also comprises and is used for final outlet that fresh culture is fed to the fresh culture import of culture tank and is used for culture soln is discharged from culture tank; But be not limited to such setting; And the fresh culture import preferably has the shape of spraying ball, and this spraying bag is drawn together bulb and the many small nozzle on this bulb, but is not limited to such setting.
In one embodiment, first cultivates part also comprises the whisking appliance that is used for mixed culture solution that is connected to culture tank inside, but is not limited to such setting.In another embodiment, first cultivates the pressure controlled valve that part also comprises the pressure that is used for controlling culture tank, and this pressure controlled valve is preferably vacuum breaker, opens through the oxygen pressure that produces in the little algae culturing process of photosynthesis.
In one embodiment, first cultivates the temperature regulator that part also comprises the temperature that is used for controlling culture tank, and this temperature regulator is preferably and is coupled to the outside water jacket of culture tank, but is not limited to such setting.
In one embodiment, first cultivates part comprises that also one or more confession transmitters are coupled to the port of culture tank, but is not limited to such setting.In another embodiment, first cultivates part also comprises and is coupled to culture tank gas being injected the gas feed of culture tank, but is not limited to such setting.
In one embodiment; Second cultivates part also comprises the oxygen escaper; Mass flow controller and the valve that is connected with mass flow controller that this oxygen escaper comprises grounding sensor (earth sensor), is electrically connected with grounding sensor, but be not limited to such setting.
In a preferred embodiment, flow speed controller is coupled to the pump part, cultivates the flow velocity of the culture soln of part with Control Circulation to the second.In a preferred embodiment, flow speed controller is built in the pump part.
In yet another aspect, the present invention provides circulating photosynthesis bio-reactor, and this bio-reactor comprises:
First cultivates part, and this first cultivates culture tank, the first substratum import, the outlet of first substratum and first light source that part comprises that substratum wherein is supplied, and said import, outlet and light source are coupled to culture tank separately;
Second cultivates part; This second cultivation part comprises that being arranged in the culture tank outside with tubular form is also exported and secondary light source by culture tube, the second substratum import, second substratum from the culture tank supplied with medium; Said import, outlet and light source are coupled to culture tube separately; Wherein the outlet of first substratum is connected with the second substratum import, and the first substratum import is connected with the outlet of second substratum, makes substratum to cultivate part and second first and cultivates circulation between the part.
The photosynthesis bio-reactor can comprise that also connecting first cultivates that part and second is cultivated part cultivates round-robin pump part between the part so that culture soln is cultivated part and second first.
In a preferred embodiment, the pump part also comprises the flow speed controller of the pump that is electrically coupled to the pump part.In a preferred embodiment, flow speed controller is built in the pump part.In a preferred embodiment, the internal diameter of culture tube is 3-30cm.In a preferred embodiment, internal diameter is 5-20cm.In most preferred embodiment, internal diameter is 10-15cm.
Circulating bioreactor of the present invention can prevent that the little algae of photosynthesis is attached at the internal surface of culture vessel, thereby can improve the culture efficiency of the little algae of photosynthesis.Therefore, can cultivate the little algae of photosynthesis economically.
In this manual, " substratum " means the substratum that is used to cultivate of the biology (like little algae) that does not contain any work, and " culture soln " means substratum and the mixture of treating cultured microalgae.
The accompanying drawing summary
Accompanying drawing combines with this specification sheets exemplary of the present invention is described, and combines in order to set forth principle of the present invention with " detailed Description Of The Invention ".
Fig. 1 is the synoptic diagram that shows bioreactor according to an embodiment of the invention.
Fig. 2 is first cultivation sectional view partly that comprises in the bioreactor of the present invention.
Fig. 3 is the plat that an embodiment of the second cultivation part that comprises in the tubular type bioreactor is described.
Fig. 4 is the synoptic diagram that an embodiment of the oxygen escaper that is coupled to culture tube is described.
Fig. 5 is the plat that explanation has a cultivation embodiment partly of two first culture soln outlets.
Detailed Description Of The Invention
In following detailed Description Of The Invention, only show and describe some exemplary embodiment of the present invention with illustrational mode.Those of skill in the art will recognize that the present invention can be with many different embodied, and should not be construed as limited to the embodiment that this paper provides.Also have, in the middle of the application's book, when certain parts be called as another parts " on " time, it can be directly on another parts, perhaps can between be connected on another parts, one or more parts are between two parties arranged between between them.In the middle of this specification sheets, same reference number is represented same parts.
The inventor has carried out various experiments, and the little algae of cultivating with research of photosynthesis is attached at the reason on the culture vessel internal surface.Results verification two reasons.First reason is thought according to the little algae propagation and the growth thereof that apply periodicity optical condition and dark condition.Another reason thinks that the little algae of photosynthesis can be coupled to the slow position of velocity of flow, because the mobile local restricted of culture soln, so sticking portion enlarges
The inventor attempts to design rate of propagation that can reduce the little algae of photosynthesis through only applying optical condition and the suitable mobile culture vessel that guarantees culture soln.But the inventor confirms, for the bioreactor of the culture tank type with whisking appliance, also can not obtain suitably flowing of culture soln even improve stirring velocity.
Therefore; The inventor attempts with additive method rather than only with whisking appliance the culture soln that contains the little algae of photosynthesis is flowed; Confirmation is recycled to the pipe that two ends all are coupled to culture tank with culture soln from culture tank, with the method for only using whisking appliance mutually specific energy that culture soln is flowed is good.Therefore, the inventor invents out culture tube and the bioreactor of pump with band light source, to address the above problem.The inventor can make culture soln flow with constant speed with the culture tube and the pump of said bioreactor, and easily culture soln is applied optical condition through the light source that is coupled to culture tube, provides competent light to cultivate the little algae of photosynthesis.
Following with reference to the bioreactor of accompanying drawing detailed description according to embodiment of the present invention.
Fig. 1 is the synoptic diagram that shows bioreactor according to an embodiment of the invention.As shown in Figure 1, bioreactor can comprise that first cultivates part 100, pump part 200 and the second cultivation part 300.The little algae of photosynthesis is recycled to pump part 200 through cultivating part 100 from first; Be recycled to second from pump part 200 and cultivate part; Be partly recirculated to the first cultivation part 100 from second cultivation then and cultivate, because the first cultivation part 100, pump part 200 and the second cultivation part 300 are interconnected.
Second cultivates part 300 can be spaced apart with the first cultivation part 100.First cultivates part 100 can as shown in Figure 1ly provide with cylinder shape, but is not limited to such setting.For example, the shape variable shape of the first cultivation part 100 is a various forms, like the Polygons post.Second cultivates part 300 can provide with tubular form, can comprise the pipe of processing with different shape, or the like.Pump part 200 is configured such that culture soln can circulation between the first cultivation part 100 and the second cultivation part 300.For example; Pump part 200 can be arranged on first and cultivate between the part 100 and the second cultivation part 300; The 3rd substratum import 210 that is connected with first culture soln outlet 132 of the first cultivation part 100 can be equipped with, and the 3rd culture soln outlet 230 that is connected with the second culture soln import 310 of the pump 220 and the second cultivation part 300 can be equipped with.Therefore, cultivate part 100 effusive culture soln from first and be transferred to the second cultivation part 300, and culture soln circulates as follows: first cultivates part 100 → pump part 200 → the second cultivates parts 300 → the first cultivation parts 100.Pump part 200 can be through cultivating the flow velocity of the culture soln of part 300 with the flow speed controller Control Circulation of its electrical coupling in second.In a preferred embodiment, flow speed controller is built in pump part 200.The flow velocity of control culture soln is important for the culture successful tenaculat Habenaria, because tenaculat Habenaria is cellulous spirrillum algae.If flow velocity is low, because the hydrokinetics situation is inappropriate, tenaculat Habenaria can take place adherent, gaseous interchange and radiation situation variation.On the contrary, high flow rate can cause the loss of useful matter in the tenaculat Habenaria.Therefore, flow velocity should suitably be controlled.Preferably with flow rate control between 1-50cm/s.More preferably, flow velocity is 10-40cm/s.In most preferred embodiment, flow velocity is 20-30cm/s.
In a preferred embodiment, the internal diameter of culture tube 330 is 3-30cm.In a preferred embodiment, internal diameter is 5-20cm.In most preferred embodiment, internal diameter is 10-15cm.The internal diameter of culture tube is important for cultivating tenaculat Habenaria.If internal diameter surpasses 30cm, because the irradiation situation is inappropriate, the productivity variation.On the contrary, if internal diameter less than 5cm, then is difficult to the amplification culture volume.
Fig. 2 be comprise in the bioreactor of the present invention first cultivate the sectional view of part 100.As shown in Figure 2, first cultivates part 100 can be equipped with cylindrical culture tank 101, inoculum import 110, gas feed 111, sensor port 120, the first culture soln import 131, first culture soln outlet 132, final outlet 133, pressure controlled valve 112, fresh culture import 134, first light source 140, whisking appliance 150 and temperature regulator 160.
For example, inoculum import 110 top, gas feed 111 and the sensor port 120 that can be coupled to culture tank 101 can be coupled to the bottom of culture tank 101.But this layout provides as an embodiment, can be according to the change of the shape of culture tank 101 and change.
Simultaneously, culture soln flows into first through the first culture soln import 131 from the second cultivation part and cultivates part 100, and cultivates part 100 to pump part 200 through first culture soln outlet, 132 outflows first.When cultivating end, final culture soln is discharged into the outside of the first cultivation part 100 through final outlet 133.
In addition; Pressure controlled valve 112 is served as reasons and is cultivated the vacuum breaker that oxygen pressure that the little algae of photosynthesis produced carries out gate (gating); It can be configured to when internal pressure remains malleation by the outside emission gases of gate, but when internal pressure descends, is closed to stop emission gases.
First light source 140 sends the device that can carry out photosynthetic light in the process of cultivating the little algae of photosynthesis for being used for, and it can send the light of three kinds of wavelength or the light of five kinds of wavelength.For this reason, preferably exposure intensity and light-dark cycle is controlled according to culture condition automatically.For example, first light source 140 can be arranged on the inside of culture tank 101.In another embodiment, if culture tank 100 comprise that some part of transparent material or culture tank 100 is equipped with can be through the transparent window of light, then first light source 140 can be arranged on the outside of culture tank 101.
Fig. 3 is the plat that an embodiment of the second cultivation part 300 that comprises in the tubular type bioreactor is described.As shown in Figure 3, second cultivate part 300 be equipped with the second culture soln import 310 that is connected with the 3rd culture soln outlet 230 of pump part 200, culture tube 330 with first cultivate part 100 second culture soln that is connected of the first culture soln import 131 export 340.
In one embodiment of the invention, the culture tube 330 of the second cultivation part 300 can constitute parallel repeatedly folded form, is included in straight part and curved part on the support, and perhaps this folded form can be piled into multilayered structure.Also have, can comprise the power supply of secondary light source 320 as this support of the shelf that is used for fixing secondary light source 320 in addition.In addition, the effect of secondary light source 320 is to emit beam to supply the little algae of photosynthesis in culturing process, to carry out photosynthesis.The wavelength that sends from secondary light source 320 preferably is similar to the three-wavelength or five wavelength of daylight, but is not limited to these wavelength, and preferably exposure intensity and light-dark cycle is controlled according to culture condition automatically.
Simultaneously, because culture tube 330 is deformed into the repeatedly pleated sheet structure that comprises straight part and curved part, the oxygen that in cultivating the little algae process of photosynthesis, produces can be accumulated in curved part and do not emitted.In order to force the oxygen of emitting accumulation, preferably culture tube is equipped with the oxygen escaper in addition at curved part.When the oxygen accumulation surpassed certain level, the oxygen of accumulation was discharged (referring to Fig. 4) through the automatic operation of oxygen escaper.
Fig. 4 is the synoptic diagram that an embodiment of the oxygen escaper that is coupled to culture tube 330 is described.As shown in Figure 4, the oxygen escaper comprises grounding sensor 351, becomes the mass flow controller 352 of electrical connection, the valve 353 that is connected with mass flow controller 352 with grounding sensor, and an end of grounding sensor 351 is immersed in the culture soln to apply electric current.
If because the accumulation of oxygen in curved part formed gas blanket, then grounding sensor 351 disengaging culture soln come out, so current interruptions.In interrupt procedure, the valve 353 that is connected with mass flow controller 352 is started, so discharge a certain amount of oxygen.When the discharging grounding sensor along with oxygen was immersed in the culture soln once more, electric current was applied in, and valve 353 stops.
In yet another aspect, tubular type bioreactor of the present invention can comprise that one is cultivated part and two or more pump part and culture tube part.
Fig. 5 is the plat of an embodiment of explanation cultivation part, and this cultivation part has 132, two the first culture soln import 131 and cultivation parts 100 that flow into culture soln from two culture tubes 330 of first culture soln outlet that two pumps of two usefulness partly provide culture soln.As shown in Figure 5, use to comprise a tubular type bioreactor of cultivating part, two or more pumps parts and culture tube part, cultivate part and culture soln partly is provided can for two culture tubes, thereby cultivate can be more efficient.
The hereinafter with reference accompanying drawing is described the operation and the effect of tubular type bioreactor in detail.
At first, with first cultivate part 100 the first culture soln import 131, the outlet 132 of first culture soln and final outlet 133 close, little algae algae kind inoculum is injected culture tank 101 through inoculum import 110 or fresh culture import 134.Apply light from first light source 140 then and cultivate for the first time, use temperature unit 160 and agitator 150 are kept suitable temperature.With various be coupled to first cultivate the transmitters inspection culture condition of sensor port 120 of part 100 after, stop first the cultivation at reasonable time.
Then, fresh culture is injected culture tank 101 through fresh culture import 134, and agitator 150 is mixed with the fresh culture of injection with the culture soln after will cultivating for the first time continuously.After will injecting culture tank 101 with the isopyknic fresh culture of first culture soln, open the first culture soln import 131 and first culture soln outlet 132, make the blended culture soln transfer to the 3rd culture soln import 210 of pump part 200.If the blended culture soln is transferred to the 3rd culture soln import 210 of pump part 200; Then the pump 220 of pump part 200 starts, and the blended culture soln is transferred to the second culture soln import 310 of the second cultivation part 300 through the 3rd culture soln outlet 230.The mixed culture solution that is transferred to the second culture soln import 310 of the second cultivation part 300 is transferred to the first culture soln import 131 of culture tube 330, second culture soln outlet, the 340 and first cultivation part 100 successively through pump 220, and circulate (first cultivates part → pump partly → second cultivates partly → first cultivation part) starts.If loop start, secondary light source 320 emit beam with irradiation culture tube 330, the little algae of photosynthesis contained in the culture soln carries out photosynthesis.The flow velocity of round-robin culture soln can be controlled with the flow speed controller that is electrically coupled to pump, with prevent the little algae of photosynthesis particularly tenaculat Habenaria take place to attach and make the photosynthesis maximization of the little algae in the circulation.Flow velocity can be controlled between the 1-50cm/s.More preferably, flow velocity is 10-40cm/s.In most preferred embodiment, flow velocity is 20-30cm/s, but is not limited to this scope.
In the circulation culturing process of the little algae of photosynthesis, through cultivating culture tank 101 injecting carbon dioxides and the nitrogen mixture body of part 100 to first, thereby apply about 0.1-1.0kg/cm to culture tank 101 via gas feed 111
2The malleation of f can prevent to derive from the various microbiological contamination of environment, and the pH of culture soln controls through the dividing potential drop of the carbonic acid gas that comprises in the adjustment mixed gas.
In addition; The oxygen that produces by the photosynthesis in the little algae culturing process of photosynthesis first cultivate part 100 culture tank 101 places from culture soln, separate after; Be transferred to the top of culture tank 101, oxygen outwards discharges through pressure controlled valve 112 then.In this situation, if second cultivate part 200 culture tube 330 be configured to be included in straight part and the parallel repeatedly folded form of curved part on the support, then oxygen can be accumulated in the curved part of culture tube 330.Therefore, preferably remove the oxygen of accumulation in the culture tube 330 through connect one or more oxygen escapers at curved part.
When termination is cultivated in the circulation of the little algae of photosynthesis, comprise the final culture soln of the little algae of photosynthesis through final outlet 133 results of opening the first cultivation part 100.After cultivate stopping, the inventor has confirmed significant result, and promptly the internal surface of culture tube 330 has 5% to be covered by the little algae of photosynthesis at the most.Therefore; The inventor only applies the rate of propagation that optical condition reduces the little algae of photosynthesis through using the light source with the culture tube assembling; And be partly recirculated to pump, be recycled to second from pump and cultivate part and cultivate from second and be partly recirculated to first and cultivate the flowability maximization that part makes culture soln through culture soln is cultivated from first, solved because the little algae of photosynthesis is attached at the problem that the culture efficiency that internal surface caused of culture vessel descends.
The little algae of photosynthesis can be that Chorella (Chlorella), Dunaliella salina belong to (Gunaliella), Spirullina (Spirulina), but is not limited to them.More preferably, the little algae of photosynthesis is a Spirullina.
Below combined some exemplary to describe the present invention; But should understand; The present invention is not limited to disclosed embodiment, thinks that on the contrary the present invention covers spirit and the various modifications in the scope that are included in appended claims and equivalent thereof and is equal to arrangement.
Claims (20)
1. bioreactor, said bioreactor comprises:
First cultivates part, and this first is cultivated part and comprise the culture tank that substratum wherein is supplied and be coupled to culture tank with the first inner light source of irradiation culture tank;
Second cultivates part, and this second is cultivated part and comprise that to be arranged on culture tank outside and by from the culture tube of culture tank supply culture soln be coupled to culture tube with the inner secondary light source of irradiation culture tube; With
Pump part, this pump part and first are cultivated part and second and are cultivated part both are connected, circulate between cultivating partly so that culture soln is cultivated part and second first.
2. the bioreactor of claim 1, wherein first light source is arranged on culture tank inside.
3. the bioreactor of claim 1, wherein secondary light source is coupled to the internal surface of culture tube or is arranged on the culture tube outside along the culture tube longitudinal direction.
4. the bioreactor of claim 1, wherein said culture tube has parallel repeatedly folded form, comprises a plurality ofly being coupled to the outside secondary light sources of culture tube along the culture tube longitudinal direction.
5. the bioreactor of claim 1; Wherein first cultivate at least one first culture soln import and at least one first culture soln outlet that part comprises that also both are connected with culture tank and pump part, second cultivates part also comprises respectively through pump part and the outlet of first culture soln and at least one the second culture soln import that is connected with the second culture soln import and at least one second culture soln outlet.
6. the bioreactor of claim 1 wherein first is cultivated part and is also comprised and be used for final outlet that fresh culture is fed to the fresh culture import of culture tank and is used for culture soln is discharged from culture tank.
7. the bioreactor of claim 1 wherein first is cultivated part and is also comprised and be coupled to the inner whisking appliance that is used for mixed culture solution of culture tank.
8. the bioreactor of claim 1 wherein first is cultivated the pressure controlled valve that part also comprises the pressure that is used for controlling culture tank.
9. the bioreactor of claim 1 wherein first is cultivated the temperature regulator that part also comprises the temperature that is used for controlling culture tank.
10. the bioreactor of claim 1 wherein first is cultivated part and is also comprised one or more sensor ports that are coupled to culture tank.
11. the bioreactor of claim 1 wherein first is cultivated part and is also comprised and be coupled to culture tank gas is injected the gas feed of culture tank.
12. the bioreactor of claim 1, wherein said pump part also comprises flow speed controller.
13. the bioreactor of claim 1, wherein the second cultivation part also comprises the oxygen escaper.
14. mass flow controller and the valve that is connected with mass flow controller that the bioreactor of claim 13, wherein said oxygen escaper comprise grounding sensor, are electrically connected with grounding sensor.
15. the bioreactor of claim 1, the internal diameter of wherein said culture tube are 3-30cm.
16. a bioreactor, said bioreactor comprises:
First cultivates part, and this first cultivates culture tank, the first substratum import, the outlet of first substratum and first light source that part comprises that substratum wherein is supplied, and said import, outlet and light source are coupled to culture tank separately;
Second cultivates part; This second cultivation part comprises that being arranged in the culture tank outside with tubular form is also exported and secondary light source by culture tube, the second substratum import, second substratum from the culture tank supplied with medium; Said import, outlet and light source are coupled to culture tube separately
Wherein the outlet of first substratum is connected with the second substratum import, and the first substratum import is connected with the outlet of second substratum, makes substratum to cultivate part and second first and cultivates circulation between the part.
17. the bioreactor of claim 16, said bioreactor also comprise with first cultivate part and second cultivate part both is connected so that culture soln first cultivate part and second cultivate partly between round-robin pump part.
18. the bioreactor of claim 17, wherein said pump partly comprises:
The 3rd culture soln import between the outlet of first culture soln and the second culture soln import; With
The 3rd culture soln outlet between the first culture soln import and the outlet of second culture soln.
19. the bioreactor of claim 16, wherein said pump part also comprises flow speed controller.
20. the bioreactor of claim 16, the internal diameter of wherein said culture tube are 3-30cm.
Priority Applications (1)
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| CN201010248859.6A CN102344888B (en) | 2010-07-29 | 2010-07-29 | Circulating type photobioreactor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344889A (en) * | 2010-07-29 | 2012-02-08 | 凯洛斯环球有限公司 | Method for cyclic culture of photosynthetic microalgae |
| CN102533541A (en) * | 2010-12-20 | 2012-07-04 | 新奥科技发展有限公司 | Microorganism culture system |
| US11680237B2 (en) | 2017-12-20 | 2023-06-20 | Univercells Technologies S.A. | Bioreactor and related methods |
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| WO2002086053A1 (en) * | 2001-04-19 | 2002-10-31 | Bioprocess A/S | Improvements relating to bioreactors |
| US20070231886A1 (en) * | 2006-03-28 | 2007-10-04 | Sartorius Ag | Reactor plant and process for culturing phototropic microorganisms |
| US20080220514A1 (en) * | 2007-03-07 | 2008-09-11 | Chao-Hui Lu | Alga microbe photosynthetic reaction system |
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
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| CN2234443Y (en) * | 1995-07-31 | 1996-09-04 | 缪坚人 | Spirulina photosynthesis reactor |
| WO2002086053A1 (en) * | 2001-04-19 | 2002-10-31 | Bioprocess A/S | Improvements relating to bioreactors |
| US20070231886A1 (en) * | 2006-03-28 | 2007-10-04 | Sartorius Ag | Reactor plant and process for culturing phototropic microorganisms |
| US20080220514A1 (en) * | 2007-03-07 | 2008-09-11 | Chao-Hui Lu | Alga microbe photosynthetic reaction system |
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344889A (en) * | 2010-07-29 | 2012-02-08 | 凯洛斯环球有限公司 | Method for cyclic culture of photosynthetic microalgae |
| CN102533541A (en) * | 2010-12-20 | 2012-07-04 | 新奥科技发展有限公司 | Microorganism culture system |
| CN102533541B (en) * | 2010-12-20 | 2015-04-15 | 新奥科技发展有限公司 | Microorganism culture system |
| US11680237B2 (en) | 2017-12-20 | 2023-06-20 | Univercells Technologies S.A. | Bioreactor and related methods |
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| CN102344888B (en) | 2014-08-20 |
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