CN102338741A - Method for measuring forest soil NO2 emission - Google Patents
Method for measuring forest soil NO2 emission Download PDFInfo
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- CN102338741A CN102338741A CN2010102316509A CN201010231650A CN102338741A CN 102338741 A CN102338741 A CN 102338741A CN 2010102316509 A CN2010102316509 A CN 2010102316509A CN 201010231650 A CN201010231650 A CN 201010231650A CN 102338741 A CN102338741 A CN 102338741A
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Abstract
The invention relates to a method for measuring forest soil NO2 emission. The method comprises the five following steps: firstly, selecting a sampling point; secondly, collecting a soil sample; thirdly, measuring the content of soil dissolved organic nitrogen (DON); fourthly, establishing a linear relation, wherein a linear relation function of forest soil NO2 emission amount and DON is: Y=0.0435X-0.1361; and fifthly, actually measuring the forest soil NO2 emission amount, wherein the content (mg.kg<-1>) of DON in the forest soil to be measured which is used as X is substituted to the function relation formula to solve for Y, i.e. the forest soil NO2 emission amount with the unit of mg.m<-2>.h<-1>. Compared with a static chamber method, the method is used for calculating the forest soil NO2 emission amount through measuring the DON, the field work load is reduced, the measuring cost is lowered, the batch quantity analysis can be carried out, the measuring work efficiency is improved, the obtained numerical error range is small, and the reliability is better.
Description
[technical field]
The present invention relates to a kind of mensuration forest soil N
2The assay method of O discharging, especially a kind of content through mensuration forest soil water-soluble nitrogen comes indirect determination forest soil N
2The assay method of O discharging.
[background technology]
N
2O is one of the strongest greenhouse gases of warming effect, and it is respectively CO that its unimolecule heats potentiality
2290 times, CH
414 times, and it can also be oxidized to NO at stratosphere, again with stratospheric O
3Reaction also makes O
3Protective seam is damaged, and makes more cosmic rays see through atmospheric envelope and also directly jeopardizes biosphere, thereby human existence health is had a strong impact on.Can know the N before the industrial revolution from the analysis of ice core
2O concentration is 0.285 μ molmol
-1, be 0.310 μ molmol now
-1, annual ratio with 0.2%~0.3% increases.N in 2000
2The greenhouse effect summation of minimum gas such as O and methane, fluorochlorohydrocarbon just and CO
2Greenhouse effect suitable, it is predicted, possibly surpass CO to the year two thousand thirty
2Greenhouse effect.
N in the atmosphere
2O 60% from the Northern Hemisphere.In each item emission source, again with agrological discharging for the highest.Therefore, the research agricultural soil comprises forest soil N
2The discharging of O, and reduce N
2The O emission measure is the focus of studying for many years always, to N
2The assay method of O discharging is one of them.
At present, N
2The assay method of O mainly is the static chamber method, and also available micrometeorology method is measured.The static chamber method is the measured surface and the sealing of living certain area with the observation case lid, makes the observation case inner air and the external world have no exchange, then the concentration of gas in the case is analyzed.The advantage of this method is the measurement of being convenient to than the gas flux of large space variability; Shortcoming is that the state of nature that closed box is known from experience measured surface produces interference; And survey area is also less relatively, measures the result and makes a variation greatly, needs repeatedly to repeat; In the collection of sample, transportation, preservation process, all possibly cause sample by problems such as severe contaminations, the sample analysis workload is big.
The microclimate method is the method through the change in concentration derivation face of land emission flux of gas of the turbulent flow conditions of measuring surface layer and minimum gas.But at present owing to lacking quick detector response, so this method is never at N
2Be applied in the observation of O emission flux, cost is very expensive again at present.
The soil water-soluble nitrogen is meant through 0.45 μ m filter opening, and can be dissolved in water, nitrogen compound with different molecular weight size.The soil water-soluble nitrogen is a most active nitrogen component in the terrestrial ecosystems, and they can be decomposed by soil microorganism, can in soil, change into other component rapidly.In timbered soil, water-soluble nitrogen is the intermediate state of SOIL ORGANIC NITROGEN storehouse mineralising, and the height of their content can influence the final product and the quantity of mineralising to a great extent.N
2The quantity that O discharges from soil depends on the conversion strength of all kinds of nitrogen compounds in mineralization rate and the soil of soil organic nitrogen compound to a great extent.Therefore, the size of soil water soluble nitrogen content can reflect forest soil N
2What of O discharge capacity.
[summary of the invention]
To soil N in the prior art
2The deficiency that the assay method of O discharging exists, the technical matters that the present invention will solve provides a kind of forest soil N
2The assay method of O discharging.Solving the problems of the technologies described above the technical scheme that is adopted is: Ben Senlin soil N
2The assay method process following steps of O discharging:
(1) selection of sampling point: need to confirm soil N
2The Forest Types and the representative location thereof of O discharging, the location in selection forest centre position, the location of eliminating roadside and limes marginis, sampling point in selected location;
(2) collection of pedotheque: around selected sampling point, gathering soil depth by serpentine 5 point sampling methods is the pedotheque 1-2kg of 0-20cm, and mixing is taken back the laboratory and analyzed;
(3) the soil water-soluble nitrogen is the assay of DON: weigh up pedotheque 20.00g, adding distil water 40ml lixiviate behind vibration 0.5h under 25 ℃, places supercentrifuge with 8000rmin
-1The centrifugal 10min of rotating speed carries out suction filtration with 0.45 μ m filter membrane, adopts nitrogen content in the alkaline alkaline potassium per-sulfate digestion determined by ultraviolet spectrophotometry filtrating;
(4) foundation of linear relationship: according to recently in two years wheat development measure the soil N of at least two kinds of forest covers respectively with the static chamber method
2O, the soil water soluble nitrogen content is measured in (3) set by step simultaneously, in EXCEL software, sets up soil N then
2The linear relationship of O discharge capacity and DON content obtains functional relation and is: Y=0.0435X-0.1361;
(5) forest soil N
2The actual measurement of O discharge capacity: will intend the forest soil of surveying, set by step (1) carry out the selection of sampling point, set by step (2) carry out sample collecting, set by step (3) measure DON content, the mgkg of unit
-1, with the described functional relation of DON content substitution step (4), make the amount of X in this formula, obtain the Y value, be the N that soil gives off
2The O amount, the mgm of unit
-2H
-1
The beneficial effect of this method is to reduce the field work amount, has reduced cost of determination, can carry out batch quantity analysis simultaneously, improves the work efficiency of measuring, and gained numerical error scope is little, and reliability is better.
[embodiment]
The present invention further details below in conjunction with embodiment: said five steps of this method, and in general, step (1) to (4) its objective is and set up functional relation that step (5) is actual measurement work for the element task before the indirect determination, both are inseparable.In case functional relation is established, only need carry out actual measurement work later on and get final product.Below the overall process of this method being done one introduces:
Existing is example with mao bamboo woods and Schima superba Qinggang evergreen broadleaf forest, and it is carried out forest soil N
2The mensuration of O discharge capacity, undertaken by following five steps:
(1) selection of sampling point: select to need to measure soil N
2The mao bamboo woods of O discharge capacity and Schima superba Qinggang evergreen broadleaf forest, then in the centre positions of this two kinds of forest covers as the representative location of measuring soil respiration.Do not select the location, roadside to be because this location receives the factor of artificial interference big, do not select the limes marginis location to be, all lack representative because be subject to the erosion of water.
(2) collection of pedotheque: around selected mao bamboo woods and Schima superba Qinggang evergreen broadleaf forest sampling point, gathering soil depth by serpentine 5 point sampling methods is the pedotheque 1-2kg of 0-20cm, and mixing is taken back the laboratory and analyzed.
(3) the soil water-soluble nitrogen is the assay of DON: pedotheque is crossed the 2m sieve, and weighing behind the mixing is the soil of 20.00g, and adding distil water 40ml lixiviate behind vibration 0.5h under 25 ℃, places supercentrifuge with 8000rmin
-1The centrifugal 10min of rotating speed carries out suction filtration with 0.45 μ m filter membrane, adopts nitrogen content in the alkaline alkaline potassium per-sulfate digestion determined by ultraviolet spectrophotometry filtrating.
(4) foundation of linear relationship: according to the soil N that measures at least two kinds of forest covers in mao bamboo woods and the Schima superba Qinggang evergreen broadleaf forest with the static chamber method respectively
2O, the soil water soluble nitrogen content is measured in (3) set by step simultaneously, in EXCEL software, sets up soil N then
2The linear relationship of O discharge capacity and DON content obtains functional relation and is: Y=0.0435X-0.1361.The soil N of this function representative
2The correlativity of O and DON content reaches the utmost point level of signifiance (R
2=0.5409; P<0.01).
(5) forest soil N
2The actual measurement of O discharge capacity: will intend mao bamboo woods and the Schima superba Qinggang evergreen broadleaf forest soil surveyed, set by step (1) carry out the selection of sampling point, set by step (2) carry out sample collecting, (3) mensuration DON content set by step, the mgkg of unit
-1, with the described functional relation of DON content substitution step (4), make the amount of X in this formula, obtain the Y value, be the N that soil gives off
2The O amount, the mgm of unit
-2H
-1
The applicant once measured the N that mao bamboo woods and Schima superba Qinggang evergreen broadleaf forest soil give off in the wheat development of 2008-2009
2The O amount, and as stated above, calculate soil N
2The O discharge capacity.With result of calculation with measure identical timbered soil N with the static chamber method
2The situation of O discharge capacity compares, and is listed in the table below, and the statistics from table can be known, at 10 times soil N
2During the O discharge capacity is measured, the soil N that this method and static chamber method are measured
2The coefficient of variation between the O discharge capacity is little, is 0.10-17.66%, and is reliable and stable, explains that this method can substitute the static chamber method to forest soil N
2The O discharge capacity is measured.
The soil N that this method and static chamber method are measured
2The O discharge capacity relatively
Claims (1)
1. forest soil N
2The assay method of O discharge capacity is characterized in that carrying out as follows:
(1) selection of sampling point: need to confirm soil N
2The Forest Types and the representative location thereof of O discharging, the location in selection forest centre position, the location of eliminating roadside and limes marginis, sampling point in selected location;
(2) collection of pedotheque: around selected sampling point, gathering soil depth by serpentine 5 point sampling methods is the pedotheque 1-2kg of 0-20cm, and mixing is taken back the laboratory and analyzed;
(3) the soil water-soluble nitrogen is the DON assay: weigh up pedotheque 20.00g, adding distil water 40ml lixiviate behind vibration 0.5h under 25 ℃, places supercentrifuge with 8000rmin
-1The centrifugal 10min of rotating speed carries out suction filtration with 0.45 μ m filter membrane, adopts nitrogen content in the alkaline alkaline potassium per-sulfate digestion determined by ultraviolet spectrophotometry filtrating;
(4) foundation of linear relationship: according to recently in two years wheat development measure the soil N of at least two kinds of forest covers respectively with the static chamber method
2The O discharge capacity, the soil water soluble nitrogen content is measured in (3) set by step simultaneously, in EXCEL software, sets up soil N then
2The linear relationship of O discharge capacity and DON content obtains functional relation and is: Y=0.0435X-0.1361;
(5) forest soil N
2The actual measurement of O discharge capacity: will intend the forest soil of surveying, set by step (1) carry out the selection of sampling point, set by step (2) carry out sample collecting, set by step (3) measure DON content, the mgkg of unit
-1, with the described functional relation of DON content substitution step (4), make the amount of X in this formula, obtain the Y value, be the N that soil gives off
2The O amount, the mgm of unit
-2H
-1
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111398565A (en) * | 2020-04-10 | 2020-07-10 | 中国农业科学院农田灌溉研究所 | Method for monitoring soil nitrogen mineralization characteristics in field in-situ state |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH10267824A (en) * | 1997-03-27 | 1998-10-09 | Agency Of Ind Science & Technol | Method for measuring dynamic contact angle of self-organization film containing sulfur organic molecules |
CN1746138A (en) * | 2004-09-08 | 2006-03-15 | 北海奥克兰生物工程有限公司 | Production of long-effective ammonium bicarbonate composite fertilizer of vegetable |
-
2010
- 2010-07-20 CN CN2010102316509A patent/CN102338741A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH10267824A (en) * | 1997-03-27 | 1998-10-09 | Agency Of Ind Science & Technol | Method for measuring dynamic contact angle of self-organization film containing sulfur organic molecules |
CN1746138A (en) * | 2004-09-08 | 2006-03-15 | 北海奥克兰生物工程有限公司 | Production of long-effective ammonium bicarbonate composite fertilizer of vegetable |
Non-Patent Citations (3)
Title |
---|
张腾宇: "长白山森林土壤N2O、CO2产生的空间变异性及其影响因素", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
杨绒等,1: "过硫酸钾氧化法测定溶液中全氮含量的影响条件研究", 《西北农林科技大学学报(自然科学版)》 * |
谢秉楼等: "覆盖与施肥处理对雷竹林土壤水溶性有机氮的影响", 《土壤学报》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111398565A (en) * | 2020-04-10 | 2020-07-10 | 中国农业科学院农田灌溉研究所 | Method for monitoring soil nitrogen mineralization characteristics in field in-situ state |
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Application publication date: 20120201 |