CN102338714A - Anisotropic nanometer press-in test table of biological soft tissues and method for performing nanometer press-in experiment by using anisotropic nanometer press-in test table - Google Patents
Anisotropic nanometer press-in test table of biological soft tissues and method for performing nanometer press-in experiment by using anisotropic nanometer press-in test table Download PDFInfo
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- CN102338714A CN102338714A CN2011101087598A CN201110108759A CN102338714A CN 102338714 A CN102338714 A CN 102338714A CN 2011101087598 A CN2011101087598 A CN 2011101087598A CN 201110108759 A CN201110108759 A CN 201110108759A CN 102338714 A CN102338714 A CN 102338714A
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Abstract
The invention discloses an anisotropic nanometer press-in test table of biological soft tissues and a method for performing a nanometer press-in experiment by using the anisotropic nanometer press-in test table. The anisotropic nanometer press-in test table of the biological soft tissues comprises a table body, wherein an accommodation hole which accommodates a test sample is formed in the middle of the upper part of the table body; two screwing cover bodies which spirally screw in the accommodation hole and cover the top of the accommodation hole are arranged on the upper part of the table body and pressed against the test sample; observation holes are formed in the screwing cover bodies; the observation holes in the screwing cover bodies respectively are circular and rectangular; and a humidity-preserving solution which preserves the humidity of the test sample can be dripped into the accommodation hole. By adoption of the anisotropic nanometer press-in test table of the biological soft tissues, the loss of the surface moisture of the test sample is avoided, the credibility of a detection experiment is improved, the error of the detection experiment is reduced, and the efficiency of the detection experiment is improved.
Description
Technical field
The present invention relates to a kind of test board, particularly relate to biologic soft tissue anisotropy nanometer and be pressed into test board
The invention still further relates to and utilize above-mentioned test board to carry out the method that nanometer is pressed into experiment.
Background technology
Current nanoscale Mechanics Performance Testing has become the strong instrument that biomaterial microstructure mechanical property detects, and has been widely used in microstructure Mechanical Properties such as tooth, bone.Yet biologic soft tissue nanoscale mechanical test but exists many problems; Matter of utmost importance is exactly the preparation and the clamping of material, and the specimen preparation mode that common soft sample adopted mainly is direct bonding and cold inlaying, and the biologic soft tissue that water cut is bigger is bonded on the sample bench; Advantage is that conventional efficient is high; It is shorter to prepare the sample required time, however bonding used cementing agent all can dewater to experiment material in various degree, the muscle that water cut was bigger after test was accomplished, blood vessel or the like become the parchment shape; And throw off with testing table, not only influence the confidence level of experiment but also strengthened error.Cold inlaying guaranteed that biomaterial can contact good with sample bench and can prevent the changing of the relative positions of material in process of press in; Yet the cold required time of inlaying can not well guarantee the biologically active of material than length, and cold inlay still to dewater to biomaterial make the mechanical property that the hardness that measures and elastic modulus all are higher than material itself.Cold inlaying is difficult to the material experiment of preserving moisture, and makes the measured surface moisture loss, and confidence level is measured in influence.
Summary of the invention
The present invention accomplishes in order to solve deficiency of the prior art; The purpose of this invention is to provide a kind of in assurance test samples biologically active; Avoid the test samples surface moisture to scatter and disappear; Improve the test experience confidence level, the biologic soft tissue anisotropy nanometer that reduces test experience error and raising test experience efficient is pressed into test board.
Biologic soft tissue anisotropy nanometer of the present invention is pressed into test board; It is characterized in that: comprise stage body; Be provided with the ccontaining hole of ccontaining specimen in the middle of the said stage body top; Said stage body top is provided with and can screws in ccontaining hole spirally and cover the spiral cover body at top, said ccontaining hole and compress said specimen, and said spiral cover body is provided with viewport, and said spiral cover body quantity is two; Viewport on the said spiral cover body is respectively circle and rectangle, can splash into the solution of preserving moisture that keeps specimen humidity in the said ccontaining hole.
Biologic soft tissue anisotropy nanometer of the present invention is pressed into test board and can also is:
Said ccontaining hole is positioned at said stage body central position.
The external wall of said spiral cover is provided with external thread, on the wall of the ccontaining hole of said stage body internal thread is set, and said internal thread is connected with said external thread spiral.
Biologic soft tissue anisotropy nanometer of the present invention is pressed into test board; Owing to comprise stage body; Be provided with the ccontaining hole of ccontaining specimen in the middle of the said stage body top; Said stage body top is provided with and can screws in ccontaining hole spirally and cover the spiral cover body at top, said ccontaining hole and compress said specimen, and said spiral cover body is provided with viewport, and said spiral cover body quantity is two; Viewport on the said spiral cover body is respectively circle and rectangle, can splash into the solution of preserving moisture that keeps specimen humidity in the said ccontaining hole.The advantage that has so in terms of existing technologies is for being immersed in the solution of moisture-keeping function owing to test sample integral body; The moisture that guarantees test samples can not scatter and disappear; Guarantee the biologically active of sample; Improve the sample setup time shortening of detection confidence and test experience, test experience efficient improves.
The present invention also provides a kind of and has utilized above-mentioned test board to carry out the method that nanometer is pressed into experiment, uses this test board to carry out nanometer and is pressed into experiment, can guarantee the moistening effect of test samples; Guarantee the biologically active of test samples; Avoid the test samples surface moisture to scatter and disappear, improve detection confidence, lower and detect error; And shorten Production Time of test sample greatly, improve detection efficiency.
Of the present inventionly utilize above-mentioned monitor station to carry out the method that nanometer is pressed into experiment; For in the ccontaining hole of said test board, splashing into a solution, the sample that detects is tiled in bottom, ccontaining hole, when the sample that detects is pressed into direction is parallel to the biological tissue top layer perpendicular to biological tissue surface or cut direction structural sheet; The sample that detects is cut into the disk shape; Be tiled in bottom, ccontaining hole, splash into a solution again, make solution cover the test samples surface fully; Use viewport to screw in ccontaining hole and compress test samples, regulate in the ccontaining hole solution to specified altitude as the spiral cover body of circle; When the sample that detects is pressed into direction perpendicular to the vertical cut surface of biomaterial surface; Test samples is cut into strip of sheet; Be tiled in bottom, ccontaining hole, splash into a solution again, make solution cover the test samples surface fully; Use viewport to screw in ccontaining hole and compress test samples, regulate solution in the ccontaining hole to specified altitude as the spiral cover body of rectangle; Then the said test board that compresses test samples is packed into and test in the card platform of nano-hardness tester.
Of the present inventionly utilize above-mentioned monitor station to carry out the method that nanometer is pressed into experiment; Because it has adopted above-mentioned monitor station and above-mentioned detection method, test samples is immersed in the biologically active that guarantees test samples in the solution, avoids the test samples surface moisture to scatter and disappear; Improve detection confidence; Reduce the error that detects, and the time of making test sample shortens the raising detection efficiency greatly.
Description of drawings
Fig. 1 biologic soft tissue anisotropy of the present invention nanometer is pressed into test board embodiment cut-open view.
Fig. 2 biologic soft tissue anisotropy of the present invention nanometer is pressed into test board embodiment vertical view.
Fig. 3 biologic soft tissue anisotropy of the present invention nanometer is pressed into the spiral cover body cut-open view of the band circular hole viewport of test board.
Fig. 4 biologic soft tissue anisotropy of the present invention nanometer is pressed into the spiral cover body vertical view of the band circular hole viewport of test board.
Fig. 5 biologic soft tissue anisotropy of the present invention nanometer is pressed into the spiral cover body cut-open view of the band rectangle viewport of test board.
Fig. 6 biologic soft tissue anisotropy of the present invention nanometer is pressed into the spiral cover body vertical view of the band rectangle viewport of test board.
Fig. 7 biologic soft tissue anisotropy of the present invention nanometer is pressed into the stage body cut-open view of test board.
Fig. 8 biologic soft tissue anisotropy of the present invention nanometer is pressed into the stage body vertical view of test board.
Fig. 9 biologic soft tissue anisotropy of the present invention nanometer is pressed into test board embodiment 1 test result figure.
Figure 10 biologic soft tissue anisotropy of the present invention nanometer is pressed into test board embodiment 2 test result figure.
Figure 11 biologic soft tissue anisotropy of the present invention nanometer is pressed into test board embodiment 3 test result figure.
The figure number explanation
1 ... Stage body 2 ... Ccontaining hole 3 ... The spiral cover body
4 ... View window
Embodiment
Biologic soft tissue anisotropy nanometer of the present invention is pressed into test board and utilizes this test board to carry out the method that nanometer is pressed into experiment and do further explain below in conjunction with accompanying drawing Fig. 1 to Figure 11.
Biologic soft tissue anisotropy nanometer of the present invention is pressed into test board; Please refer to Fig. 1 to Fig. 8; Comprise stage body 1, be provided with the ccontaining hole 2 of ccontaining specimen in the middle of said stage body 1 top, said stage body 1 top is provided with the spiral cover body 3 that can screw in 2 covering 2 tops, said ccontaining hole, ccontaining hole spirally and compresses said specimen; Said spiral cover body 3 is provided with viewport; Said spiral cover body 3 quantity are two, and the viewport on the said spiral cover body 3 is respectively circle and rectangle, can splash into the solution of preserving moisture that keeps specimen humidity in the said ccontaining hole 2.Like this, the advantage that has in terms of existing technologies guarantees that for because test sample can whole be immersed in the solution of moisture-keeping function the moisture of test samples can not scatter and disappear; Guarantee the biologically active of sample; Improve detection confidence and, screw in spiral cover body 3 then and compress sample, and splash into solution again and can the strength youngster accomplish test sample owing to make test sample and only need splash into solution and add test samples and splash into sample again; The sample setup time shortening of test experience, test experience efficient improves.
Certainly, biologic soft tissue anisotropy nanometer of the present invention is pressed into test board, and further optimized technical scheme is that said ccontaining hole 2 is positioned at said stage body 1 central position.Shift position easy to detect like this, and convenient location.Concrete in addition spiral cover body 3 can be that said spiral cover body 3 outer walls are provided with external thread with the integrated structure in ccontaining hole 2, on 2 walls of said stage body 1 ccontaining hole internal thread is set, and said internal thread is connected with said external thread spiral.Certainly can also be other integrated structure, test samples is compressed get final product so long as spiral cover body 3 can screw in ccontaining hole 2.
Of the present inventionly utilize above-mentioned test board to carry out the method that nanometer is pressed into experiment; The sample that detects is pressed into direction and is parallel to the structural sheet on biological tissue top layer perpendicular to biological tissue surface or cut direction, and the sample that detects is cut into the disk shape, is tiled in 2 bottoms, ccontaining hole; Splash into a solution again; Make solution cover the test samples surface fully, use viewport to screw in ccontaining hole 2 and compress test samples, regulate ccontaining hole 2 interior solution to specified altitude as the spiral cover body 3 of circle; When the sample that detects is pressed into direction perpendicular to the vertical cut surface of biomaterial surface; Test samples is cut into strip of sheet; Be tiled in 2 bottoms, ccontaining hole, splash into a solution again, make solution cover the test samples surface fully; Use viewport to screw in ccontaining holes 2 and compress test samples, regulate solution in the ccontaining hole 2 to specified altitude as the spiral cover body of rectangle 3; Then the said test board that compresses test samples is packed into and test in the card platform of nano-hardness tester.Through above-mentioned steps, can produce test sample with the shortest time, and test sample upper surface and lower surface all there is the solution of preserving moisture, therefore, guarantee the biologically active of test samples, improve the confidence level that detects, reduce the error that detects, improve detection efficiency.
Bulk cartilage on the test experience material gigot hone lamella; Be pressed into direction perpendicular to the cartilage natural surface; The test experience material is cut into 15mm diameter disk as test samples, in the ccontaining hole 2 of monitor station, splashes into a solution of preserving moisture, and test samples is tiled in 2 bottoms, ccontaining hole; Drip solution again; Use the spiral cover body 3 of band circular hole view window 4 to push down test samples then, regulate solution height in the spiral cover body 3, then the integrated testability platform is packed on the Ka Tai of nano-hardness tester through view window 4 usefulness droppers.The Flat end cone pressure head of selecting for use pressure head to produce when being pressed into for Agilent company, the surface is 40% near sensitivity coefficient, and compression distance is 5000nm, and the result who is obtained is as shown in Figure 9.
Bulk cartilage on the test experience material gigot hone lamella; Be pressed into the vertical cut surface of direction perpendicular to the cartilage natural surface; The test experience material is cut into slices as test samples with the band shape that microtome is cut into 6mmX15mm, in the ccontaining hole 2 of monitor station, splash into a solution of preserving moisture, test samples is tiled in 2 bottoms, ccontaining hole; Drip solution again; Use the spiral cover body 3 of band rectangle view window 4 to push down test samples then, regulate solution height in the spiral cover body 3, then the integrated testability platform is packed on the Ka Tai of nano-hardness tester through view window 4 usefulness droppers.The Flat end cone pressure head of selecting for use pressure head to produce when being pressed into for Agilent company, the surface is 40% near sensitivity coefficient, and compression distance is 5000nm, and the result who is obtained is shown in figure 10.
Embodiment 3:
The test experience material is the OX-heart blood vessel, is pressed into direction perpendicular to the core barrel inside surface, and the test experience material is cut into 15mm diameter disk as test samples; Because core barrel is thinner, the atresia aluminium flake with thick 2mm screws in the ccontaining hole 2 as pad in advance, the core barrel sample is put into again; In the ccontaining hole 2 of monitor station, splash into a solution of preserving moisture; Test samples is tiled in 2 bottoms, ccontaining hole, drips solution again, use then and push down test samples with the spiral cover body 3 of circular view window 4; Regulate solution height in the spiral cover body 3 through view window 4 usefulness droppers, then the integrated testability platform is packed on the Ka Tai of nano-hardness tester.The Flat end cone pressure head of selecting for use pressure head to produce when being pressed into for Agilent company, the surface is 10% near sensitivity coefficient, and compression distance is 5000nm, and the result who is obtained is shown in figure 11.
Above-mentionedly only several kinds of specific embodiments among the present invention are explained; But can not be as protection scope of the present invention; Every equivalence variation of having done according to the design spirit among the present invention or modification or equal proportion are amplified or are dwindled etc., all should think to fall into protection scope of the present invention.
Claims (6)
1. biologic soft tissue anisotropy nanometer is pressed into test board; It is characterized in that: comprise stage body; Be provided with the ccontaining hole of ccontaining specimen in the middle of the said stage body top; Said stage body top is provided with and can screws in ccontaining hole spirally and cover the spiral cover body at top, said ccontaining hole and compress said specimen, and said spiral cover body is provided with viewport, and said spiral cover body quantity is two; Viewport on the said spiral cover body is respectively circle and rectangle, can splash into the solution of preserving moisture that keeps specimen humidity in the said ccontaining hole.
2. biologic soft tissue anisotropy nanometer according to claim 1 is pressed into test board, it is characterized in that: said ccontaining hole is positioned at said stage body central position.
3. biologic soft tissue anisotropy nanometer according to claim 1 and 2 is pressed into test board, it is characterized in that: the external wall of said spiral cover is provided with external thread, on the wall of the ccontaining hole of said stage body internal thread is set, and said internal thread is connected with said external thread spiral.
4. biologic soft tissue anisotropy nanometer according to claim 1 and 2 is pressed into test board, it is characterized in that: bottom, said ccontaining hole pad is provided with pad.
5. utilize claim 1 or 2 described test boards to carry out the method that nanometer is pressed into experiment; It is characterized in that: concrete steps are following: in the ccontaining hole of said test board, splash into a solution, the sample that detects is tiled in bottom, ccontaining hole, when the sample that detects is pressed into direction is parallel to the biological tissue top layer perpendicular to biological tissue surface or cut direction structural sheet; The sample that detects is cut into the disk shape; Be tiled in bottom, ccontaining hole, splash into a solution again, make solution cover the test samples surface fully; Use viewport to screw in ccontaining hole and compress test samples, regulate in the ccontaining hole solution to specified altitude as the spiral cover body of circle; When the sample that detects is pressed into direction perpendicular to the vertical cut surface of biomaterial surface; Test samples is cut into strip of sheet; Be tiled in bottom, ccontaining hole, splash into a solution again, make solution cover the test samples surface fully; Use viewport to screw in ccontaining hole and compress test samples, regulate solution in the ccontaining hole to specified altitude as the spiral cover body of rectangle; Then the said test board that compresses test samples is packed into and test in the card platform of nano-hardness tester.
6. utilize claim 1 or 2 described test boards to carry out the method that nanometer is pressed into experiment, it is characterized in that: before experiment, be encased inside pad in bottom, ccontaining hole.
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| CN2011101087598A CN102338714A (en) | 2011-03-07 | 2011-04-28 | Anisotropic nanometer press-in test table of biological soft tissues and method for performing nanometer press-in experiment by using anisotropic nanometer press-in test table |
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| CN2011101087598A CN102338714A (en) | 2011-03-07 | 2011-04-28 | Anisotropic nanometer press-in test table of biological soft tissues and method for performing nanometer press-in experiment by using anisotropic nanometer press-in test table |
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Cited By (9)
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| CN102589974A (en) * | 2012-02-22 | 2012-07-18 | 北京工业大学 | Eyeball microstructure nano-indentation experiment table and method of nano-indentation experiment |
| CN103575591A (en) * | 2013-10-14 | 2014-02-12 | 北京工业大学 | Articular cartilage microstructure nanoindentation inlaying and water-retaining sample stage and experiment method |
| CN103575872A (en) * | 2013-10-14 | 2014-02-12 | 北京工业大学 | Moisturizing sample stage and experimental method for testing mechanical property through cell nanoindentation |
| CN104483516A (en) * | 2014-12-18 | 2015-04-01 | 中国空间技术研究院 | Conversion clamp for testing light-emitting devices |
| CN107907398A (en) * | 2017-11-15 | 2018-04-13 | 北京工业大学 | A kind of leveling method for being used for permanent depth nano impress experiment |
| CN107907433A (en) * | 2017-11-15 | 2018-04-13 | 北京工业大学 | Nano impress representative region is micro- to rotate leveling platform |
| CN107941638A (en) * | 2017-11-15 | 2018-04-20 | 北京工业大学 | Heterogeneous material each component performance test methods based on nano impress method |
| CN107976375A (en) * | 2017-11-15 | 2018-05-01 | 北京工业大学 | Micro- rotation levelling device that a kind of multiple spot is held out against |
| CN109520837A (en) * | 2018-12-19 | 2019-03-26 | 南京医科大学附属口腔医院 | Nano-hardness tester loading bench special bio soft tissue moisturizing device |
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| CN102589974A (en) * | 2012-02-22 | 2012-07-18 | 北京工业大学 | Eyeball microstructure nano-indentation experiment table and method of nano-indentation experiment |
| CN102589974B (en) * | 2012-02-22 | 2014-01-01 | 北京工业大学 | Eyeball microstructure nano-indentation experiment table and method of nano-indentation experiment |
| CN103575591A (en) * | 2013-10-14 | 2014-02-12 | 北京工业大学 | Articular cartilage microstructure nanoindentation inlaying and water-retaining sample stage and experiment method |
| CN103575872A (en) * | 2013-10-14 | 2014-02-12 | 北京工业大学 | Moisturizing sample stage and experimental method for testing mechanical property through cell nanoindentation |
| CN104483516A (en) * | 2014-12-18 | 2015-04-01 | 中国空间技术研究院 | Conversion clamp for testing light-emitting devices |
| CN104483516B (en) * | 2014-12-18 | 2018-04-10 | 中国空间技术研究院 | Luminescent device test conversion fixture |
| CN107907398A (en) * | 2017-11-15 | 2018-04-13 | 北京工业大学 | A kind of leveling method for being used for permanent depth nano impress experiment |
| CN107907433A (en) * | 2017-11-15 | 2018-04-13 | 北京工业大学 | Nano impress representative region is micro- to rotate leveling platform |
| CN107941638A (en) * | 2017-11-15 | 2018-04-20 | 北京工业大学 | Heterogeneous material each component performance test methods based on nano impress method |
| CN107976375A (en) * | 2017-11-15 | 2018-05-01 | 北京工业大学 | Micro- rotation levelling device that a kind of multiple spot is held out against |
| CN107941638B (en) * | 2017-11-15 | 2020-05-08 | 北京工业大学 | Method for testing performance of each component of non-uniform material based on nano scratch method |
| CN107907398B (en) * | 2017-11-15 | 2020-05-19 | 北京工业大学 | Leveling method for constant-depth nano scratch experiment |
| CN107907433B (en) * | 2017-11-15 | 2020-05-19 | 北京工业大学 | Nano indentation typical region micro-rotation leveling platform |
| CN109520837A (en) * | 2018-12-19 | 2019-03-26 | 南京医科大学附属口腔医院 | Nano-hardness tester loading bench special bio soft tissue moisturizing device |
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Application publication date: 20120201 |