CN102337220B - Penicillium purpurogenum DB1 strain and preparation and application thereof - Google Patents

Penicillium purpurogenum DB1 strain and preparation and application thereof Download PDF

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CN102337220B
CN102337220B CN 201110254279 CN201110254279A CN102337220B CN 102337220 B CN102337220 B CN 102337220B CN 201110254279 CN201110254279 CN 201110254279 CN 201110254279 A CN201110254279 A CN 201110254279A CN 102337220 B CN102337220 B CN 102337220B
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flax
penicillium purpurogenum
jute
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丁若垚
刘国亮
董政娥
黎征帆
郁崇文
张兴群
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Donghua University
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Abstract

The invention relates to a penicillium purpurogenum DB1 strain and preparation and application thereof. A preparation method of the strain comprises the following steps of: (1) putting rotten seaweed into an enriched medium for stationary culture at room temperature, coating the enriched medium onto an isolation medium, and stationary culture for 1-4 days at 30 DEG C to obtain a wild flax biological treatment strain; (2) inoculating the strain obtained in the step (1) into a flax lignin enriched medium, and culturing at 28 DEG C for 72 hours; and (3) selecting a strain with lignin degrading capability from the flax lignin enriched medium to obtain the penicillium purpurogenum DB1 strain. The penicillium purpurogenum DB1 strain can be applied to preparation processes of flax, hemp, jute or red jute fiber for spinning by degumming with peroxides. The strain has the advantages of short growing period, difficultly polluted, low treatment cost, good fiber quality after treatment, mild reaction condition, high heat resistance and environmental friendliness; and the strain has a simple process and is suitable for mass industrial production.

Description

A kind of penicillium purpurogenum DB 1 strain and preparation thereof and application
Technical field
The invention belongs to penicillium purpurogenum bacteria strain and preparation thereof and Application Areas, particularly a kind of penicillium purpurogenum DB 1 strain and preparation thereof and application.
Background technology
Fiber crops are one of textile raw materials, and its kind mainly contains flax, ramie, hemp, jute, bluish dogbane, kendir, sisal hemp, abaca, nettle and piemarker etc.Flaxen fiber is the general designation of the fiber obtained from various numb plants, has advantages of that moisture absorption, ventilative, heat radiation and other natural fibers such as antibiotic are incomparable.China is as one of the abundantest country of hemp resource in the world, and the annual flaxen fiber of producing and goods thereof also as the export of commodities in large amounts U.S., West Europe and country in Southeast Asia, are earned foreign exchange nearly 10,000,000,000 dollars except satisfying the demand of domestic market every year.Reduce discharging policy and human consumer's back to nature at national energy-saving, pursue under the green consumption idea promotion, bast-fibre green processing technology has become the study hotspot of field of textiles.
The chief component of flax, hemp, jute and bastose is Mierocrystalline cellulose, also has in addition the non-cellulose materials such as more hemicellulose, pectin and xylogen to be wrapped in the flaxen fiber surface.The non-cellulose materials such as these colloids, particularly xylogen are cementing in the fiber outside, so that raw ramie is firm sheet strip.So raw ramie must be removed the colloids such as xylogen by the process of coming unstuck together in spinning process, to satisfy spinning requirement.At present, the kiering method that bast industry is commonly used mainly is chemical process, namely utilizes strong acid and strong base that raw ramie or numb rove are processed.The chemical degumming technique energy consumption is large, equipment loss is large, and the waste water of discharging can't recycle, and pollution problem is serious.
Summary of the invention
Technical problem to be solved by this invention provides a kind of penicillium purpurogenum (Penicillium purpurogenum) DB1 bacterial strain and application thereof, this strain growth cycle is short, bacterial classification is difficult for contaminated, processing cost is low, the mild condition of coming unstuck, contamination resistance is strong, and resistance toheat is good, non-environmental-pollution, fiber quality is good after processing; Its preparation method is simple, Production Flow Chart is short, be fit to large-scale industrial production.
A kind of penicillium purpurogenum of the present invention (Penicillium purpurogenum) DB1 bacterial strain, its 18S rRNA gene order is as follows:
GCACTCTTTTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTACCCTACTACATGGATACCTGTGGTAATTCTAGAGCTAATACATGCGCAAAACCCCGACTTCGGAAGGGGTGTATTTATTAGATAAAAAACCAATGCCCTTCGGGGCTCCTTGGTGATTCATAATAACTTCACGAATCGCATGGCCTTGCGCCGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGCAACGGGTAACGGGGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGATACGGGGAGGTAGTGACAATAAATACTGATACAGGGCTCTTTTGGGTCTTGTAATTGGAATGAGAACAATCTAAATCCCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGCCCGTCCTGCCGGTCCGCCTCACCGCGAGTACTGGTCCGGATGGGCCTTTCTTTCTGGGGAATCCCATGGCCTTCACTGGCTGTGGCGGGGAACCAGGACTTTTACTGTGAAAAAATTAGAGTGTTCAAAGCAGGCCTTTGCTCGGATACATTAGCATGGAATAATAGAATAGGACGTGCGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGGGGGCGTCAGTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACTACTGCGAAAGCATTCGCCAAGGATGTTTTCATTAATCAGGGAACGAAAGTTAGGGGATCGAAGACGATCAGATCCCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGGCGGGGTTTCTATGATGACCCGCTCGGCACCTTACGAGAAATCAAAGTTTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACAAGGCGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGGCCCTTAAATAGCCCGGTCCGCGTTTGCGGGCCGCTGGCTTCTTAGGGGACTATCGCT。
Above-mentioned penicillium purpurogenum (Penicillium purpurogenum) DB1 bacterial strain, feature is as follows: vegetative mycelium is colourless, the distinct color of light color or tool; Mycelia has tabula, and conidiophore also has tabula, and is smooth or coarse; Base portion amacrine, top do not form the top capsule that expands, and its conidiophore produces several take turns symmetrical or asymmetric stigmas through branch repeatedly, and shape such as broom are called broom shape body; Conidium sphere, ellipse or short cylindrical, smooth or coarse, be blue-greenish colour during most of growth; Potato potato substratum: colony growth rate is slightly fast, cultivates diameter 8cm after 14 days, and the conidium structure is a large amount of, surperficial greyish-green, and edge white, quality is fine and close flocculence, the back side is colourless; Conidiophore stem 70-250 μ m * 2.5-3.5 μ m, wall is smooth, and expand usually on the top; The penicillus two-wheel is given birth to, and is close to each other and is bordering on parallel; Metulae one is taken turns 4-8,9.0-13 * 2.5-3.0 μ m; Lanceolar, the stalk neck is obvious, and conidium presents ellipse, 2.8-3.5 * 2.2-3.0 μ m, wall is level and smooth.
The preserving number of penicillium purpurogenum provided by the invention (Penicillium purpurogenum) DB1 bacterial strain is CGMCCNo.4694, preservation day is on March 17th, 2011, and the suggestion Classification And Nomenclature is: penicillium purpurogenum (Penicillium purpurogenum).
According to 18sR dna sequencing (such as Fig. 5-9) Blast and cluster analysis result, this bacterium and penicillium purpurogenum similarity are 99%, in conjunction with physiological and biochemical test result and microscopy analysis, naming this bacterial strain is penicillium purpurogenum (Penicillium purpurogenum) DB1 bacterial strain.
The preparation method of a kind of penicillium purpurogenum DB 1 strain of the present invention comprises:
(1) sea grass of will rotting places the enrichment medium room temperature to leave standstill in the ratio of 1g: 20ml to cultivate 30 days, and then got the 0.1ml enrichment medium and coat in the isolation medium, and 30 ℃ leave standstill and cultivated 1～4 day, obtain wild-type flax biological treatment dominant strain;
(2) with the inoculation that obtains in the step (1) in the Flax Lignin nutritional medium, cultivate 72h for 28 ℃;
(3) from above-mentioned Flax Lignin nutritional medium, select the bacterial strain with lignin degrading ability.
Enrichment culture based formulas in the described step (1) is: flax powder 5g, tap water 200ml, pH nature; The separation and Culture based formulas is: flax powder 5g, (NH 4) 2SO 45g, K 2HPO 41g, MgSO 40.5g, KCl 0.5g, FeSO 40.01, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator.
The component of the Flax Lignin nutritional medium in the described step (2) comprises: Flax Lignin 2g, NaNO 33g, K 2HPO 40.5g, MgSO 41g, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator.
A kind of penicillium purpurogenum DB 1 strain of the present invention is weaved with the application in the phloem fiber techniques such as flax, hemp, jute or bluish dogbane in the peroxide degumming preparation.
Described applying step comprises:
(1) penicillium purpurogenum DB 1 strain is stored in potato dextrose agar, 30 ℃ of 220rpm cultivate 48h, add frozen damping fluid; Frozen damping fluid is by potassium primary phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and Trisodium Citrate 0.0588g, bitter salt 0.02645g, glycerine 10ml adds water and is settled to 100ml and makes;
(2) in the cooled 50mL potato glucose substratum of sterilization, bacterium one ring of access step (1) gained, shake-flask culture 48h under rotating speed 220r/min, 30 ℃ of conditions of temperature;
(3) culture medium inoculated with step (2) gained enters Flax Lignin fermention medium 5L, and shake-flask culture 48h obtains zymocyte liquid under rotating speed 220r/min, 30 ℃ of conditions of temperature; Wherein, flax fermentative medium formula is: flax raw ramie xylogen 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO 40.05g, FeSO 40.001g, (NH 4) 2SO 40.5g moisturizing is to the 100ml constant volume;
(4) flax, hemp, jute or the kenaf raw material after will sterilizing mixed by bath raio with step (3) gained zymocyte liquid at 1: 20, at 30 ℃, to process respectively in the 160-220rpm shaking table 1～4 day, the treatment time is then, remove fermented liquid, obtain flax, hemp, jute or bastose;
(5) above-mentioned flax, hemp, jute or bastose and come unglued liquid are pressed mixing, under 70-100 ℃ of temperature, processed 1-8 hour, then washing, and method routinely oils, drying, namely obtaining can be for the fiber of weaving; Wherein flax, hemp, jute or bastose are 1 to the weight ratio of come unglued liquid: 8-1: 25, and the temperature of coming unstuck is 70-100 ℃, the time is 1-8 hour.
Flax, hemp, jute or kenaf raw material described in the step (4) is former stem, yarn or the fabric of fiber crops.
Come unglued liquid described in the step (5) is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water; The weight ratio 1 of wherein peroxide degumming agent, sequestrant and hydrogen bond disrupting agent sum and the solution that comes unstuck: 8-1: 25.
Peroxide degumming agent described in the step (5) is hydrogen peroxide, Potassium Persulphate, Sodium Persulfate, ammonium persulphate, antihypo, SPC-D, percarbonic acid ammonium, potassium per(oxy)borate, Sodium peroxoborate or ammonium pertorate, and its consumption is the 0.25-8% of flax, hemp, jute or bastose weight.
Sequestrant described in the step (5) is sodium phosphate, tripoly phosphate sodium STPP, one or more of sodium-metaphosphate, Sodium phosphate dibasic, SODIUM PHOSPHATE, MONOBASIC, citric acid, disodium ethylene diamine tetraacetate, its consumption is the 0.1-4% of flax, hemp, jute or bastose weight; The hydrogen bond disrupting agent is urea, and its consumption is the 0.05-2% of flax, hemp, jute or bastose weight.
The DNA that extracts bacterial strain carries out pcr amplification, selects fungi 18S rRNA gene universal primer sequence (5-ATT GGAGGG CAA GTC TGG TG-3 and 5-CCG ATC CCT AGT CGG CAT AG-3); And then record bacterial strain 18SrRNA complete sequence, as follows:
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGAAAAGGAGCTTGCTCCTTTGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTACCCTATAGTTTGGGATAACTCCGGGAAACCGGGGCTAATACCGAATAATCTCTTTTGCTTCATGGCAAGAGACTGAAAGACGGCATCTCGCTGTCGCTATAAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGTTTTCGGATCGTAAAACTCTGTTGTAAGGGAAGAACAAGTACAGTAGTAACTGGCTGTACCTTGACGGTACCTTATTAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCCTTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCAAGTGTAGCGGTGAAATGCGTAGAGATTTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCGTTGACCACTGTAGAGATATAGTTTCCCCTTCGGGGGCAACGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGATACAAACGGTTGCCAACTCGCGAGAGGGAGCTAATCCGATAAAGTCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGATAGATGATTGGGGTGAAGTCGTAACAAGGTAACC。
Under the culture condition that makes up, this strain culturing condition is extensive, good heat resistance, and growth and breeding is fast, can effectively remove the colloids such as xylogen in flax, hemp, jute or the bastose, and enzyme is alive stable, bacterium, the equal nontoxicity of enzyme.This bacterial strain and culture systems can be directly used in flax retting.
After bacterial classification pre-treatment or the processing, the friendly type superoxide of real-world environment carries out inactivation treatment, to substitute traditional boiling water inactivation technology, with water saving; Numb sample after the strong oxidizing property of utilizing simultaneously superoxide in the inactivation technology is processed bacterial classification carries out refining and bleaching, with further Optimization Technology, energy-saving and emission-reduction.
Bacterial strain of the present invention has the ability to phloem fiber lignin degradations such as flax, hemp, jute or bluish dogbanes, can be applied to the phloem fiber surface modification.The invention provides the Bio-Pretreatment that bacterial strain and culture systems can be directly used in the phloem fiber textile process such as flax, hemp, jute or bastose.The invention provides bacterial strain, to have a growth cycle short, and bacterial classification is difficult for contaminated, and processing cost is low, the mild condition of coming unstuck, and contamination resistance is strong, and resistance toheat is good, non-environmental-pollution, the advantages such as fiber quality is good after processing; Compared with prior art, the present invention is applied to the deactivation of bacterial classification, refining and the bleaching process of flaxen fiber with environmentally friendly superoxide, and three operations are merged into an operation.Therefore the present invention has that technique is simple, Production Flow Chart is short, is fit to the characteristics such as large-scale industrial production, and to carry out the Bio-Pretreatment that xylogen is removed before phloem fiber surface modification and the textile process under mass production conditions significant for exploring penicillium purpurogenum.
Beneficial effect
(1) the invention provides bacterial strain and culture systems, the high yield lignin-degrading enzymes can be directly used in flax retting after testing, it is short to have a cycle of coming unstuck, and lignin removing rate reaches 70-80%, and the fiber dispersion rate reaches 100%, respond well to flax retting, the efficient of coming unstuck reaches 95%, and bacterial classification is difficult for contaminated, processing cost is low, fiber quality is good, the mild condition of coming unstuck, and contamination resistance is strong, resistance toheat is good, the advantages such as non-environmental-pollution;
(2) the present invention has the characteristics such as simple, the suitable large-scale industrial production of technique.Utilizing this system to carry out flax retting, to have a usually time short, and the flax fiber scatter coefficient reaches 100%, and degumming rate reaches more than 90%, and the degummed ramie quality is good.
Description of drawings
Fig. 1 is microscope dyeing photo (oily mirror);
Fig. 2 is potato potato culture bacterium colony photo;
Fig. 3 is fungi treating processes photo;
Fig. 4 is flax fiber photo after processing;
Fig. 5-9 is the 18SrDNA sequence of penicillium purpurogenum DB 1 strain.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
The screening of flax retting bacterial classification obtains
(1) will rot first sea grass and enrichment medium leaves standstill in ratio room temperature in enrichment medium of 1g: 20ml and cultivated 30 days, then get the 0.1ml enrichment medium and coat isolation medium, 30 ℃ leave standstill cultivation 1～4 day, obtain wild-type flax biological treatment dominant strain;
Wherein, the enrichment culture based formulas is: flax powder 5g, tap water 200ml, pH nature; The separation and Culture based formulas is: flax powder 5g, (NH 4) 2SO 45g, K 2HPO 41g, MgSO 40.5g, KCl 0.5g, FeSO 40.01, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;
(2) with the inoculation that obtains in the step (1) in the Flax Lignin nutritional medium, cultivate 72h for 28 ℃.The Flax Lignin nutritional medium, its component comprises: Flax Lignin 2g, NaNO 33g, K 2HPO 40.5g, MgSO 41g, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;
(3) from above-mentioned substratum, select the bacterial strain with lignin degrading ability.
Embodiment 2
The preparation of zymocyte liquid:
(1) penicillium purpurogenum DB 1 strain is stored in potato dextrose agar, and 30 ℃ of 220rpm cultivate 48h, add frozen damping fluid; Frozen damping fluid is by potassium primary phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and Trisodium Citrate 0.0588g, bitter salt 0.02645g, glycerine 10ml adds water and is settled to 100ml and makes.
(2) in the cooled 50mL potato glucose substratum of sterilization, bacterium one ring of access step (1) gained, shake-flask culture 48h under rotating speed 220r/min, 30 ℃ of conditions of temperature;
(3) culture medium inoculated with step (2) gained enters Flax Lignin fermention medium 5L, and shake-flask culture 48h obtains zymocyte liquid under rotating speed 220r/min, 30 ℃ of conditions of temperature; Wherein, flax fermentative medium formula is: flax raw ramie xylogen 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO 40.05g, FeSO 40.001g, (NH 4) 2SO 40.5g moisturizing is to the 100ml constant volume.
Embodiment 3
Flax retting technique is as follows:
The 5g flax straw is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of flax straw and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed come unglued liquid, stops biological treatment.Flax straw after the biological treatment is carried out inactivation treatment, its technique is: the flax straw after the biological treatment and come unglued liquid mix with 1: 10 ratio, and under 70 ℃ condition, processed 3 hours, wherein the consumption of superoxide is 3% of flax straw, amount of urea is 0.05% of flax straw, the consumption of tripoly phosphate sodium STPP is 2% of flax straw, and the consumption of disodium ethylene diamine tetraacetate is 0.1% of flax straw.2130 weaving flax fiber can making after coming unstuck.
Embodiment 4
Hemp degumming process is as follows:
Hemp fibre after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of hemp and embodiment 2, liquid amount is 100ml, at 35 ℃, comes unstuck respectively in the 200rpm shaking table 2 days; Usually time is then removed come unglued liquid, stops biological treatment.Hemp after the biological treatment is carried out inactivation treatment, its technique is: the hemp after the biological treatment and come unglued liquid mix with 1: 25 ratio, and under 90 ℃ condition, processed 1 hour, wherein the consumption of superoxide is 5% of hemp, amount of urea is 1% of hemp, the consumption of Sodium phosphate dibasic is 2% of hemp, and the consumption of SODIUM PHOSPHATE, MONOBASIC is 2% of hemp.1190 weaving hemp fibre can making after coming unstuck.
Embodiment 5
Jute degumming technique is as follows:
Jute after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of jute and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 4 days; Usually time is then removed come unglued liquid, stops biological treatment.Jute after the biological treatment is carried out inactivation treatment, its technique is: the jute after the biological treatment and come unglued liquid mix with 1: 8 ratio, and under 100 ℃ condition, processed 8 hours, wherein the consumption of superoxide is 8% of jute, amount of urea is 2% of the former stem of jute, the consumption of sodium-metaphosphate is 2% of the former stem of jute, and the consumption of disodium ethylene diamine tetraacetate is 2% of jute, and the consumption of tripoly phosphate sodium STPP is 2% of jute.820 weaving jute fibre can making after coming unstuck.
Embodiment 6
The linen thread and yarn boiling is as follows:
Linen thread and yarn (flax roving or second hards) after the 5g sterilization is inserted the 250ml triangular flask, zymocyte liquid with linen thread and yarn and embodiment 2 is sub-packed in the triangular flask by bath raio at 1: 20, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed come unglued liquid, stops biological treatment.Linen thread and yarn after the biological treatment is carried out inactivation treatment, its technique is: the linen thread and yarn after the biological treatment and come unglued liquid mix with 1: 15 ratio, and under 90 ℃ condition, processed 2 hours, wherein the consumption of superoxide is 2% of linen thread and yarn, amount of urea is 0.05% of linen thread and yarn, the consumption of tripoly phosphate sodium STPP is 2% of linen thread and yarn, and the consumption of sodium phosphate is 1% of linen thread and yarn.1530 weaving linen thread and yarn can making after coming unstuck.
Embodiment 7
The sodolin biological treatment is as follows:
Sodolin after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of flax straw and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed come unglued liquid, stops biological treatment.Sodolin after the biological treatment is carried out inactivation treatment, its technique is: the sodolin after the biological treatment and come unglued liquid mix with 1: 10 ratio, and under 85 ℃ condition, processed 1 hour, wherein the consumption of superoxide is 2.5% of sodolin, amount of urea is 1% of sodolin, the consumption of sodium phosphate is 3% of sodolin, and the consumption of disodium ethylene diamine tetraacetate is 1% of sodolin, and the consumption of citric acid is 1% of sodolin.After coming unstuck, sodolin whiteness, pliability are significantly improved, and prodding and itching feeling significantly lowers.
Figure IDA0000087405030000011
Figure IDA0000087405030000021
Figure IDA0000087405030000031
Figure IDA0000087405030000041

Claims (2)

  1. A penicillium purpurogenum ( Penicillium purpurogenum) the DB1 bacterial strain, its preserving number is CGMCC No.4694.
  2. 2. a kind of penicillium purpurogenum DB 1 strain as claimed in claim 1 is weaved with the application in the technique of flax, hemp, jute or bastose in the peroxide degumming preparation.
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