CN102329737B - Eupenicillium javanicum DB4 strain as well as preparation and application thereof - Google Patents

Abstract

The invention relates to a Eupenicillium javanicum DB4 strain as well as preparation and application thereof. The preparation method comprises the following steps of: (1) placing rotten seaweed in an enrichment culture medium, standing and culturing at room temperature, coating the enrichment culture medium on a separation culture medium, and standing and culturing at the temperature of 30 DEG C for 1-4 days to obtain wild flax bio-treated strain; (2) inoculating the strain obtained in the step (1) to a flax lignin nutrient culture medium and culturing at the temperature of 28 DEG C for 72 hours; and (3) selecting the strain with lignin degrading capability from the flax lignin nutrient culture medium. The obtained strain can be applied to the processes for preparing flax, hemp, jute or red ramie fibers for textile by peroxide degumming. The strain has the advantages of short growth period, low pollution probability, low treatment cost, mild reaction conditions, strong pollution resistance, good heat resistance, no environmental pollution, and good fiber quality after treatment; and the process is simple and is suitable for large-scale industrial production.

Description

A kind of Eupenicillium javanicum DB4 bacterial strain and preparation and application

Technical field

The invention belongs to Penicillium notatum bacterial strain and preparation thereof and Application Areas, particularly a kind of Eupenicillium javanicum DB4 bacterial strain and preparation and application.

Background technology

Fiber crops are one of textile raw materials, and its kind mainly contains flax, ramie, hemp, jute, bluish dogbane, kendir, sisal hemp, abaca, nettle and piemarker etc.Flaxen fiber is the general designation of the fiber obtained from various numb plants, has moisture absorption, ventilative, heat radiation and the incomparable advantage of other natural fibers such as antibiotic.China is as one of the abundantest country of hemp resource in the world, and the annual flaxen fiber of producing and goods thereof also as the export of commodities in large amounts U.S., West Europe and country in Southeast Asia, are earned foreign exchange nearly 10,000,000,000 dollars except satisfying the demand of domestic market every year.Reduce discharging policy and human consumer's back to nature at national energy-saving, pursue under the green consumption idea promotion, bast-fibre green processing technology has become the study hotspot of field of textiles.

The chief component of flax, hemp, jute and bastose is Mierocrystalline cellulose, also has in addition the non-cellulose materials such as more hemicellulose, pectin and xylogen to be wrapped in the flaxen fiber surface.The non-cellulose materials such as these colloids, particularly xylogen are cementing in the fiber outside, so that raw ramie is firm sheet strip.So raw ramie must be removed the colloids such as xylogen by the process of coming unstuck together in spinning process, to satisfy spinning requirement.At present, the kiering method that bast industry is commonly used mainly is chemical process, namely utilizes strong acid and strong base that raw ramie or numb rove are processed.The chemical treatment technology energy consumption is large, and the waste water of the large and discharging of equipment loss can't recycle, and pollution problem is serious.

Summary of the invention

Technical problem to be solved by this invention provides a kind of Eupenicillium javanicum (Eupenicillium javanicum) DB4 bacterial strain and preparation and application, this bacterial strain has the ability to phloem fiber lignin degradations such as flax, hemp, jute or bluish dogbanes, its preparation method technique is simple, is fit to large-scale industrial production.

A kind of Eupenicillium javanicum DB4 bacterial strain of the present invention, its 18S rRNA gene order is as follows:

CACTTTATACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTA

CCTTACTACATGGATACCTGTGGTAATTCTAGAGCTAATACATGCTAAAAACCCCGACT

TCAGGAAGGGGTGTATTTATTAGATAAAAAACCAACGCCCTTCGGGGCTCCTTGGTGA

ATCATAATAACTTAACGAATCGCATGGCCTTGCGCCGGCGATGGTTCATTCAAATTTCT

GCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGCAACGGGTAACGGG

GAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA

AGGCAGCAGGCGCGCAAATTACCCAATCCCGATACGGGGAGGTAGTGACAATAAATA

CTGATACGGGGCTCTTTCGGGTCTCGTAATTGGAATGAGAACAATTTAAATCCCTTAA

CGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA

TAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGTCTGGCT

GGCCGGTCCGCCTCACCGCGAGTACTGGTCCGGCTGGACCTTTCCTTCTGGGGAACC

TCATGGCCTTCACTGGCTGTGGGGGGAACCAGGACTTTTACTGTGAAAAAATTAGAG

TGTTCAAAGCAGGCCTTTGCTCGAATACATTAGCATGGAATAATAGAATAGGACGTGC

GGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGGGGGCG

TCAGTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACTACTGCGA

AAGCATTCGCCAAGGATGTTTTCATTAATCAGGGAACGAAAGTTAGGGGATCGAAGA

CGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGACGGGA

TTCTATGATGACCCGTTCGGCACCTTACGAGAAATCAAAGTTTTTGGGTTCTGGGGGG

AGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACAAGGCGT

GGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACAAAAT

AAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGTT

CTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGGCCCT

TAAATAGCCCGGTCCGCATTTGCGGGCCGCTGGC。

Eupenicillium javanicum provided by the invention (Eupenicillium javanicum) DB4 bacterial strain, feature is as follows: vegetative mycelium is colourless, the distinct color of light color or tool; Mycelia has tabula, and conidiophore also has tabula, and is smooth or coarse; Base portion amacrine, top do not form the top capsule that expands, and its conidiophore produces several take turns symmetrical or asymmetric stigmas through branch repeatedly, and shape such as broom are called broom shape body; Conidium sphere, ellipse or short cylindrical, smooth or coarse, be blue-greenish colour during most of growth; Potato potato substratum: colony growth rate is slightly fast, cultivates diameter 8cm after 14 days, and the conidium structure is a large amount of, surperficial greyish-green, and edge white, quality is fine and close flocculence, the back side is colourless; Conidiophore stem 70-250 μ m * 2.5-3.5 μ m, wall is smooth, and expand usually on the top; The penicillus two-wheel is given birth to, and is close to each other and is bordering on parallel; Metulae one is taken turns 4-8,9.0-13 * 2.5-3.0 μ m; Lanceolar, the stalk neck is obvious, and conidium presents ellipse, 2.8-3.5 * 2.2-3.0 μ m, wall is level and smooth.

The preserving number of Eupenicillium javanicum of the present invention (Eupenicillium javanicum) DB4 bacterial strain is CGMCC No.4696, preservation day is on March 17th, 2011, the suggestion Classification And Nomenclature is Java penicillium (Eupenicillium javanicum), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, the address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.

According to 18sR dna sequencing (such as Fig. 5-9) Blast and cluster analysis result, this bacterium and Eupenicillium javanicum similarity are 99%, in conjunction with physiological and biochemical test result and microscopy analysis, naming this bacterial strain is Eupenicillium javanicum (Eupenicillium javanicum) DB4 bacterial strain.

A kind of preparation method of Eupenicillium javanicum DB4 bacterial strain comprises:

(1) sea grass of will rotting places enrichment medium in the ratio of 1g: 20ml, and room temperature leaves standstill cultivated 30 days, then gets the 0.1ml enrichment medium and coats in the isolation medium, and 30 ℃ leave standstill and cultivated 1～4 day, obtain wild-type flax biological treatment bacterial strain;

(2) with the inoculation that obtains in the step (1) in the Flax Lignin nutritional medium, cultivate 72h for 28 ℃;

(3) from above-mentioned Flax Lignin nutritional medium, select the bacterial strain with lignin degrading ability, and get final product.

Enrichment culture based formulas in the described step (1) is: flax powder 5g, tap water 200ml, pH nature; The separation and Culture based formulas is: flax powder 5g, (NH ₄) ₂SO ₄5g, K ₂HPO ₄1g, MgSO ₄0.5g, KCl 0.5g, FeSO ₄0.01, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator.

The component of the Flax Lignin nutritional medium in the described step (2) comprises: Flax Lignin 2g, NaNO ₃3g, K ₂HPO ₄0.5g, MgSO ₄1g, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator.

A kind of Eupenicillium javanicum DB4 bacterial strain of the present invention is weaved with the application in flax, hemp, jute or the bastose technique in the peroxide degumming preparation.

Described applying step comprises:

(1) Eupenicillium javanicum DB4 bacterial strain is stored in the potato dextrose agar, 30 ℃ of 220rpm cultivate 48h, add frozen damping fluid; Wherein frozen damping fluid is by potassium primary phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and Trisodium Citrate 0.0588g, bitter salt 0.02645g, glycerine 10ml adds water and is settled to 100ml and makes;

(2) in the cooled 50mL potato glucose substratum of sterilization, bacterium one ring of access step (1) gained, shake-flask culture 48h under rotating speed 220r/min, 30 ℃ of conditions of temperature;

(3) culture medium inoculated with step (2) gained enters Flax Lignin fermention medium 5L, and shake-flask culture 48h obtains zymocyte liquid under rotating speed 220r/min, 30 ℃ of conditions of temperature; Wherein, flax fermentative medium formula is: flax raw ramie xylogen 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO ₄0.05g, FeSO ₄0.001g, (NH ₄) ₂SO ₄0.5g moisturizing is to the 100ml constant volume;

(4) flax, hemp, jute or the kenaf raw material after will sterilizing mixed by bath raio with step (3) gained zymocyte liquid at 1: 20, at 30 ℃, processed respectively in the 160-220rpm shaking table 1～4 day, and then removed fermented liquid, obtain flax, hemp, jute or bastose;

(5) with after above-mentioned flax, hemp, jute or bastose and the come unglued liquid mixing, under 30-100 ℃ of temperature, processed 1-5 hour, then washing, method routinely oils, drying, namely obtaining can be for the fiber of weaving; Wherein flax, hemp, jute or bastose are 1 to the weight ratio of come unglued liquid: 10-1: 20, and the temperature of coming unstuck is 30-100 ℃, the time is 1-5 hour.

Flax, hemp, jute or kenaf raw material described in the step (4) is former stem, yarn or the fabric of fiber crops.

Come unglued liquid described in the step (5) is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water; The weight ratio 1 of wherein peroxide degumming agent, sequestrant and hydrogen bond disrupting agent sum and the solution that comes unstuck: 8-1: 25.

Peroxide degumming agent described in the step (5) is hydrogen peroxide, Potassium Persulphate, Sodium Persulfate, ammonium persulphate, antihypo, SPC-D, percarbonic acid ammonium, potassium per(oxy)borate, Sodium peroxoborate or ammonium pertorate, and its consumption is the 0.25-8% of flax, hemp, jute or bastose weight.

Sequestrant described in the step (5) is sodium phosphate, tripoly phosphate sodium STPP, one or more of sodium-metaphosphate, Sodium phosphate dibasic, SODIUM PHOSPHATE, MONOBASIC, citric acid, disodium ethylene diamine tetraacetate, its consumption is the 0.1-4% of flax, hemp, jute or bastose weight; The hydrogen bond disrupting agent is urea, and its consumption is the 0.05-2% of flax, hemp, jute or bastose weight.

The DNA that extracts bacterial strain carries out pcr amplification, selects fungi 18S rRNA gene universal primer sequence (5-ATT GGA GGG CAA GTC TGG TG-3 and 5-CCG ATC CCT AGT CGG CAT AG-3); And then record bacterial strain 18SrRNA complete sequence, as follows:

CACTTTATACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA

GTACCTTACTACATGGATACCTGTGGTAATTCTAGAGCTAATACATGCTAAAAACCCCG

ACTTCAGGAAGGGGTGTATTTATTAGATAAAAAACCAACGCCCTTCGGGGCTCCTTGG

TGAATCATAATAACTTAACGAATCGCATGGCCTTGCGCCGGCGATGGTTCATTCAAATT

TCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGCAACGGGTAAC

GGGGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAA

GGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGATACGGGGAGGTAGTGACAATAA

ATACTGATACGGGGCTCTTTCGGGTCTCGTAATTGGAATGAGAACAATTTAAATCCCTT

AACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTC

CAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGTCTG

GCTGGCCGGTCCGCCTCACCGCGAGTACTGGTCCGGCTGGACCTTTCCTTCTGGGGA

ACCTCATGGCCTTCACTGGCTGTGGGGGGAACCAGGACTTTTACTGTGAAAAAATTA

GAGTGTTCAAAGCAGGCCTTTGCTCGAATACATTAGCATGGAATAATAGAATAGGACG

TGCGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGGGG

GCGTCAGTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACTACTG

CGAAAGCATTCGCCAAGGATGTTTTCATTAATCAGGGAACGAAAGTTAGGGGATCGA

AGACGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGACG

GGATTCTATGATGACCCGTTCGGCACCTTACGAGAAATCAAAGTTTTTGGGTTCTGGG

GGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACAAGG

CGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACAA

AATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCC

GTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGGC

CCTTAAATAGCCCGGTCCGCATTTGCGGGCCGCTGGC。

Under the culture condition that makes up, this strain culturing condition is extensive, good heat resistance, and growth and breeding is fast, can effectively remove the colloids such as xylogen in flax, hemp, jute or the bastose, and enzyme is alive stable, bacterium, the equal nontoxicity of enzyme.This bacterial strain and culture systems can be directly used in flax retting.

After bacterial classification pre-treatment or the processing, the friendly type superoxide of real-world environment carries out inactivation treatment, to substitute traditional boiling water inactivation technology, with water saving; Numb sample after the strong oxidizing property of utilizing simultaneously superoxide in the inactivation technology is processed bacterial classification carries out refining and bleaching, with further Optimization Technology, energy-saving and emission-reduction.

Bacterial strain of the present invention has the ability to phloem fiber lignin degradations such as flax, hemp, jute or bluish dogbanes, can be applied to the phloem fiber surface modification.The invention provides the Bio-Pretreatment that bacterial strain and culture systems can be directly used in the phloem fiber textile process such as flax, hemp, jute or bluish dogbane.The invention provides bacterial strain and have the growth cycle weak point, bacterial classification is difficult for contaminated, and processing cost is low, and fiber quality is good after processing, the mild condition of coming unstuck, and contamination resistance is strong, and resistance toheat is good, the advantages such as non-environmental-pollution; Compared with prior art, the present invention have technique simple, be fit to the characteristics such as large-scale industrial production, to carry out the Bio-Pretreatment that xylogen is removed before phloem fiber surface modification and the textile process under mass production conditions significant for exploring Eupenicillium javanicum.

Beneficial effect

(1) the invention provides bacterial strain and culture systems, simultaneously high yield polygalacturonase and hemicellulase can be directly used in flax retting after testing, have the cycle of coming unstuck weak point, the fiber dispersion rate reaches 100%, and is respond well to flax retting, the efficient of coming unstuck reaches 95%, bacterial classification is difficult for contaminated, and processing cost is low, and fiber quality is good, the mild condition of coming unstuck, contamination resistance is strong, and resistance toheat is good, the advantages such as non-environmental-pollution;

(2) the present invention has the characteristics such as simple, the suitable large-scale industrial production of technique.Utilize this system to carry out flax retting and have the usually time weak point, the flax fiber scatter coefficient reaches 100%, and degumming rate reaches more than 90%, and the degummed ramie quality is good.

Description of drawings

Fig. 1 is microscope dyeing photo (oily mirror);

Fig. 2 is potato potato culture bacterium colony photo;

Fig. 3 is fungi treating processes photo;

Fig. 4 is flax fiber photo after processing;

Fig. 5-9 is the 18SrDNA sequence of Eupenicillium javanicum DB4 bacterial strain.

Embodiment

Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.

Embodiment 1

The screening of flax retting bacterial classification obtains

(1) from the rotten sea grass of East Sea Zhoushan sea area sampling, first sea grass and enrichment medium are left standstill in ratio room temperature in enrichment medium of 1g: 20ml and cultivated 30 days, then get the 0.1ml enrichment medium and coat isolation medium, 30 ℃ leave standstill cultivation 1～4 day, obtain wild-type flax biological treatment dominant strain;

Wherein, the enrichment culture based formulas is: flax powder 5g, tap water 200ml, pH nature; The separation and Culture based formulas is: flax powder 5g, (NH ₄) ₂SO ₄5g, K ₂HPO ₄1g, Mg _SO ₄0.5g, KCl 0.5g, FeSO ₄0.01, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;

(2) with the inoculation that obtains in the step (1) in the Flax Lignin nutritional medium, cultivate 72h for 28 ℃.The Flax Lignin nutritional medium, its component comprises: Flax Lignin 2g, NaNO ₃3g, K ₂HPOx 0.5g, MgSO ₄1g, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;

(3) from above-mentioned substratum, select the bacterial strain with lignin degrading ability.

Embodiment 2

The preparation of zymocyte liquid:

(1) the Eupenicillium javanicum DB4 bacterial strain with embodiment 1 gained is stored in the potato dextrose agar, and 30 ℃ of 220rpm cultivate 48h, add frozen damping fluid; Wherein frozen damping fluid is by potassium primary phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and Trisodium Citrate 0.0588g, bitter salt 0.02645g, glycerine 10ml adds water and is settled to 100ml and makes;

(2) in the cooled 50mL potato glucose substratum of sterilization, bacterium one ring of access step (1) gained, shake-flask culture 48h under rotating speed 220r/min, 30 ℃ of conditions of temperature;

(3) culture medium inoculated with step (2) gained enters Flax Lignin fermention medium 5L, and shake-flask culture 48h obtains zymocyte liquid under rotating speed 220r/min, 30 ℃ of conditions of temperature; Wherein, flax fermentative medium formula is: flax raw ramie xylogen 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO ₄0.05g, FeSO ₄0.001g, (NH ₄) ₂SO ₄0.5g moisturizing is to the 100ml constant volume.

Embodiment 3

Flax retting technique is as follows:

The 5g flax straw is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of flax straw and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed come unglued liquid, stops biological treatment.Flax straw after the biological treatment is carried out inactivation treatment, its technique is: the flax straw after the biological treatment and come unglued liquid mix with 1: 10 ratio, and under 70 ℃ condition, processed 3 hours, wherein the consumption of superoxide is 3% of flax straw, amount of urea is 0.05% of flax straw, the consumption of tripoly phosphate sodium STPP is 2% of flax straw, and the consumption of disodium ethylene diamine tetraacetate is 0.1% of flax straw.2130 weaving flax fiber can making after coming unstuck.

Embodiment 4

Hemp yarn boiling is as follows:

Hemp yarn after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of hemp yarn and embodiment 2, liquid amount is 100ml, at 35 ℃, comes unstuck respectively in the 200rpm shaking table 2 days; Usually time is then removed come unglued liquid, stops biological treatment.Hemp yarn after the biological treatment is carried out inactivation treatment, its technique is: the hemp yarn after the biological treatment and come unglued liquid mix with 1: 25 ratio, and under 90 ℃ condition, processed 1 hour, wherein the consumption of superoxide is 5% of hemp yarn weight, amount of urea is 1% of hemp yarn weight, the consumption of Sodium phosphate dibasic is 2% of hemp yarn weight, and the consumption of SODIUM PHOSPHATE, MONOBASIC is 2% of hemp yarn weight.The weaving of rear preparation of coming unstuck is soft with the hemp yarn, whiteness is high, end breakage rate is little, intensity is high.

Embodiment 5

The Jute thread and yarn boiling is as follows:

Jute thread and yarn after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of Jute thread and yarn and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 4 days; Usually time is then removed come unglued liquid, stops biological treatment.Jute thread and yarn after the biological treatment is carried out inactivation treatment, its technique is: the Jute thread and yarn after the biological treatment and come unglued liquid mix with 1: 8 ratio, and under 100 ℃ condition, processed 8 hours, wherein the consumption of superoxide is 8% of Jute thread and yarn weight, amount of urea is 2% of Jute thread and yarn weight, the consumption of sodium-metaphosphate is 2% of Jute thread and yarn weight, and the consumption of disodium ethylene diamine tetraacetate is 2% of Jute thread and yarn weight, and the consumption of tripoly phosphate sodium STPP is 2% of Jute thread and yarn weight.760 weaving Jute thread and yarn can making after coming unstuck.

Embodiment 6

The sodolin biological treatment is as follows:

Sodolin after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of sodolin and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed come unglued liquid, stops biological treatment.Sodolin after the biological treatment is carried out inactivation treatment, its technique is: the sodolin after the biological treatment and come unglued liquid mix with 1: 10 ratio, and under 85 ℃ condition, processed 1 hour, wherein the consumption of superoxide is 2.5% of sodolin weight, amount of urea is 1% of sodolin weight, the consumption of sodium phosphate is 3% of sodolin weight, and the consumption of disodium ethylene diamine tetraacetate is 1% of sodolin weight, and the consumption of citric acid is 1% of sodolin weight.After coming unstuck, sodolin whiteness, pliability are significantly improved, and prodding and itching feeling significantly lowers.

Claims (2)

1. an Eupenicillium javanicum (Eupenicillium javanicum) DB4 bacterial strain, the preserving number of this bacterial strain is CGMCC No.4696, its 18S rRNA gene order is as follows:

CACTTTATACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTA

CCTTACTACATGGATACCTGTGGTAATTCTAGAGCTAATACATGCTAAAAACCCCGACT

TCAGGAAGGGGTGTATTTATTAGATAAAAAACCAACGCCCTTCGGGGCTCCTTGGTGA

ATCATAATAACTTAACGAATCGCATGGCCTTGCGCCGGCGATGGTTCATTCAAATTTCT

GCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGCAACGGGTAACGGG

GAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA

AGGCAGCAGGCGCGCAAATTACCCAATCCCGATACGGGGAGGTAGTGACAATAAATA

CTGATACGGGGCTCTTTCGGGTCTCGTAATTGGAATGAGAACAATTTAAATCCCTTAA

CGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA

TAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGTCTGGCT

GGCCGGTCCGCCTCACCGCGAGTACTGGTCCGGCTGGACCTTTCCTTCTGGGGAACC

TCATGGCCTTCACTGGCTGTGGGGGGAACCAGGACTTTTACTGTGAAAAAATTAGAG

TGTTCAAAGCAGGCCTTTGCTCGAATACATTAGCATGGAATAATAGAATAGGACGTGC

GGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGGGGGCG

TCAGTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACTACTGCGA

AAGCATTCGCCAAGGATGTTTTCATTAATCAGGGAACGAAAGTTAGGGGATCGAAGA

CGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGACGGGA

TTCTATGATGACCCGTTCGGCACCTTACGAGAAATCAAAGTTTTTGGGTTCTGGGGGG

AGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACAAGGCGT

GGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACAAAAT

AAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGTT

CTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGGCCCT

TAAATAGCCCGGTCCGCATTTGCGGGCCGCTGGC。

2. a kind of Eupenicillium javanicum DB4 bacterial strain as claimed in claim 1 is weaved with the application in the technique of flax, hemp, jute or bastose in the peroxide degumming preparation.

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