CN102323225B - 一种检测低密度脂蛋白胆固醇的方法和试剂盒 - Google Patents
一种检测低密度脂蛋白胆固醇的方法和试剂盒 Download PDFInfo
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- CN102323225B CN102323225B CN 201110153416 CN201110153416A CN102323225B CN 102323225 B CN102323225 B CN 102323225B CN 201110153416 CN201110153416 CN 201110153416 CN 201110153416 A CN201110153416 A CN 201110153416A CN 102323225 B CN102323225 B CN 102323225B
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- 238000000034 method Methods 0.000 title claims abstract description 49
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title abstract description 32
- 108010007622 LDL Lipoproteins Proteins 0.000 title abstract description 20
- 102000007330 LDL Lipoproteins Human genes 0.000 title abstract description 20
- 239000003153 chemical reaction reagent Substances 0.000 title abstract description 19
- HVYWMOMLDIMFJA-UHFFFAOYSA-N 3-cholesterol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 HVYWMOMLDIMFJA-UHFFFAOYSA-N 0.000 title abstract 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims abstract description 28
- 229920001817 Agar Polymers 0.000 claims abstract description 21
- IYDGMDWEHDFVQI-UHFFFAOYSA-N phosphoric acid;trioxotungsten Chemical compound O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O IYDGMDWEHDFVQI-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000012086 standard solution Substances 0.000 claims abstract description 20
- 229910001629 magnesium chloride Inorganic materials 0.000 claims abstract description 14
- 239000000725 suspension Substances 0.000 claims abstract description 11
- 238000002835 absorbance Methods 0.000 claims abstract description 10
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910001425 magnesium ion Inorganic materials 0.000 claims abstract description 6
- 108010028554 LDL Cholesterol Proteins 0.000 claims description 55
- 238000012360 testing method Methods 0.000 claims description 36
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 22
- 229920002538 Polyethylene Glycol 20000 Polymers 0.000 claims description 21
- 210000002966 serum Anatomy 0.000 claims description 21
- 230000009849 deactivation Effects 0.000 claims description 9
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- 239000000203 mixture Substances 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 20
- 238000006243 chemical reaction Methods 0.000 abstract description 19
- 239000007788 liquid Substances 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 238000004879 turbidimetry Methods 0.000 abstract description 3
- 239000002202 Polyethylene glycol Substances 0.000 abstract 2
- 229920001223 polyethylene glycol Polymers 0.000 abstract 2
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 239000003795 chemical substances by application Substances 0.000 description 10
- 235000012000 cholesterol Nutrition 0.000 description 10
- 238000001556 precipitation Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000004094 surface-active agent Substances 0.000 description 5
- 108010010234 HDL Lipoproteins Proteins 0.000 description 4
- 102000015779 HDL Lipoproteins Human genes 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 208000024172 Cardiovascular disease Diseases 0.000 description 3
- 108010004103 Chylomicrons Proteins 0.000 description 3
- 108010023302 HDL Cholesterol Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 208000026106 cerebrovascular disease Diseases 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000002526 effect on cardiovascular system Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 229920000447 polyanionic polymer Polymers 0.000 description 3
- 102000006395 Globulins Human genes 0.000 description 2
- 108010044091 Globulins Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
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- 238000000975 co-precipitation Methods 0.000 description 2
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- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 102000000853 LDL receptors Human genes 0.000 description 1
- 108010001831 LDL receptors Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000003143 atherosclerotic effect Effects 0.000 description 1
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- 210000003712 lysosome Anatomy 0.000 description 1
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- 229920002521 macromolecule Polymers 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 108010071584 oxidized low density lipoprotein Proteins 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
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- 239000000126 substance Substances 0.000 description 1
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- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
方法 | 波长 | 反应方向 | 温度 | 测量时间 | 标准液浓度 | 样品量 | 线性 |
终点法 | 630nm | + | 37℃ | 300s | 2.6mmol/L | 5μL | 1.3-5.2mmol/L |
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CN 201110153416 CN102323225B (zh) | 2011-06-09 | 2011-06-09 | 一种检测低密度脂蛋白胆固醇的方法和试剂盒 |
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CN 201110153416 CN102323225B (zh) | 2011-06-09 | 2011-06-09 | 一种检测低密度脂蛋白胆固醇的方法和试剂盒 |
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CN102323225A CN102323225A (zh) | 2012-01-18 |
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CN102749459A (zh) * | 2012-07-27 | 2012-10-24 | 北京恩济和生物科技有限公司 | 一种脂蛋白(a)检测试剂盒及其制备方法 |
CN106596209B (zh) * | 2015-10-14 | 2020-05-12 | 杭州量康科技有限公司 | 干血样本预处理及检测方法 |
CN108949902A (zh) * | 2018-08-31 | 2018-12-07 | 山东博科生物产业有限公司 | 一种血清小而密低密度脂蛋白胆固醇检测试剂盒 |
CN109580303A (zh) * | 2018-12-06 | 2019-04-05 | 潍坊泽成生物技术有限公司 | 低密度脂蛋白的制备方法 |
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CN100443884C (zh) * | 2005-06-29 | 2008-12-17 | 中生北控生物科技股份有限公司 | 低密度脂蛋白胆固醇的定量测定方法、试剂及试剂盒 |
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