CN102293322A - Bioactive protein peptide feed containing Chinese herbal medicine, its preparation method and its application - Google Patents
Bioactive protein peptide feed containing Chinese herbal medicine, its preparation method and its application Download PDFInfo
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- CN102293322A CN102293322A CN2011102003778A CN201110200377A CN102293322A CN 102293322 A CN102293322 A CN 102293322A CN 2011102003778 A CN2011102003778 A CN 2011102003778A CN 201110200377 A CN201110200377 A CN 201110200377A CN 102293322 A CN102293322 A CN 102293322A
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Abstract
The invention relates to the field of feed processing, and especially relates to a feed for livestock and aquatic animals, its preparation and its application. The invention provides a bioactive protein peptide feed containing Chinese herbal medicine, which comprises the following components by weight percentage: 80%-90% of bioactive protein peptide feed raw material, 0.5%-10% of plantago or plantago extract and 0.5%-10% of inula herb or inula herb extract. The bioactive protein peptide feed has reasonable formula which combines the advantages and characteristics of active protein peptidase and Chinese herbal medicine, and is capable of raising the conversion rate of the feed, and preventing as well as treating diseases. Thereby the incidence rate of the livestock and the production cost can be reduced, so that the bioactive protein peptide feed provides the healthy product for markets and raises the culture profit.
Description
Technical field
The present invention relates to belong to field of feed processing, relate in particular to a kind of livestock and poultry, aquatic animal feed and preparation thereof and purposes.
Technical background
Traditional aquaculture model uses antibiosis usually to control transmission in a large number, causes the medicament residue of livestock product to exceed standard, even resistance, thereby influences the quality of livestock product.Because antibiotic many side effects, particularly the consumer is to the concern of food-safety problem in recent years, and antibiotic use is being forbidden or limited in countries in the world all one after another, and accelerate development and popularization is novel, safe, green feed additive efficiently.
Along with the foundation of oligopeptides absorption approach, the biologically active protein, peptide (product of vegetable protein aerobic bacteria-anaerobic bacteria group technology fermentation.Mainly contain digestible, in micromolecular protein, plant polypeptide, protease etc.), with its unique biological function, obtained in fields such as food, medicine, cosmetics using widely.In recent years also become the focus of Animal nutrition area research gradually.Biologically active protein, peptide combines the advantage of polypeptide and digestive ferment, animal body had nutrition widely and physiological regulatory action, and can degrade fully in animal body, have safe in utilization, have no side effect, characteristics such as applied range, meet the development of modern biological industry and the requirement of livestock birds health cultivation, for feed has already been opened up new development space.But biologically active protein, peptide also has some shortcomings to the control of diseases of bird and livestock, in case that fowl poultry in prevention from suffering from the diseases, is treated is not prompt enough, can cause heavy economic losses.
Summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art part, first purpose of the present invention provides a kind of biologically active protein, peptide feed that contains Chinese herbal medicine, this feed combines the advantage and the characteristics of activated protein peptase and Chinese herbal medicine, plays the conversion ratio that improves feed, and reaches the effect of preventing and curing diseases.Second purpose of the present invention provides the preparation method of the above-mentioned biologically active protein, peptide feed that contains Chinese herbal medicine.Second purpose of the present invention provides the application of the above-mentioned biologically active protein, peptide feed that contains Chinese herbal medicine.
In order to realize first above-mentioned purpose, the present invention has adopted following technical scheme:
A kind of biologically active protein, peptide feed that contains Chinese herbal medicine, this feed is made of following component by weight percentage:
Biologically active protein, peptide feedstuff 80%-99%,
Asiatic plantain or Herba Plantaginis extract 0.5%-10%,
Gold grass or the gold careless extract 0.5%-10% that boils that boils.
As preferably, this feed is made of following component by weight percentage:
Biologically active protein, peptide feedstuff 80%-94%,
Asiatic plantain 3%-10%,
The gold careless 3%-10% that boils.
As preferably, this feed is made of following component by weight percentage:
Biologically active protein, peptide feedstuff 90%-99%,
Herba Plantaginis extract 0.5%-5%,
The gold careless extract 0.5%-5% that boils.
In order to realize second above-mentioned purpose, the present invention has adopted following technical scheme:
A kind ofly prepare the described method that contains the biologically active protein, peptide feed of Chinese herbal medicine, this method comprises the steps: 1) take by weighing required Asiatic plantain and the gold grass that boils on request, 2) Asiatic plantain and gold are boiled grass meal is broken to 40-60 order, 3) mix with the biologically active protein, peptide feedstuff.
Perhaps, above-mentioned method comprises the steps: 1) take by weighing required Asiatic plantain and the gold grass that boils, 2 on request) medicinal material is extracted with 70%-95% ethanol water, be concentrated into dried, 3) mix with the biologically active protein, peptide feedstuff.
In order to realize the 3rd above-mentioned purpose, the present invention has adopted following technical scheme:
The biologically active protein, peptide feed that contains Chinese herbal medicine of above-mentioned each technical scheme is used for preparing the application of the feed of control livestock and poultry or aquatic livestock diarrhoea, antiviral or breathing problem.As the feed that preferably is used to prepare control livestock and poultry or aquatic livestock diarrhoea.
Asiatic plantain of the present invention has reducing fever and causing diuresis, cool blood, detoxication, can prevent and treat fowl poultry diarrhoea.The gold grass that boils has inhibitory action to harmful bacterium such as staphylococcus aureus, streptococcus pneumonia, Pseudomonas aeruginosa and Escherichia coli, and is inhibited to virus simultaneously, can improve the disease of anti-virus ability and its respiratory system of control of fowl poultry.
The present invention is owing to adopted above-mentioned technical scheme, and feed formula is reasonable, combines the advantage and the characteristics of activated protein peptase and Chinese herbal medicine, plays the conversion ratio that improves feed, and reaches the effect of preventing and curing diseases.Thereby reduce the incidence probability of fowl poultry, reduce production costs,, improve culture benefit for market provides health-oriented products.
The specific embodiment
The invention will be further described below in conjunction with embodiment, but be not limited in embodiment.
Embodiment 1
Contain the biologically active protein, peptide feed of Chinese herbal medicine, its component is: 1) biologically active protein, peptide feedstuff, Asiatic plantain, the gold grass that boils; 2) of the present invention consisting of: biologically active protein, peptide is 90%, and Asiatic plantain is 5%, and the gold grass that boils is 5%.
Described preparation method carries out: 1) take by weighing required Chinese medicine, 2 on request as follows) with pulverizing medicinal materials to 40-60 order, 3) mix with activated protein peptase feedstuff.
Embodiment 2
Contain the biologically active protein, peptide feed of Chinese herbal medicine, its component is: 1) biologically active protein, peptide feedstuff, Herba Plantaginis extract, the gold careless extract that boils; 2) of the present invention consisting of: biologically active protein, peptide is 97%, and Herba Plantaginis extract is 2%, and the gold careless extract that boils is 1%.
Described preparation method, carry out as follows: 1) take by weighing required Asiatic plantain, the gold grass that boils on request, 2) medicinal material is used the 80% ethanol water refluxing extraction three times of 10 times of volumes respectively, filter, filtrate is concentrated into dried, and 3) mix with activated protein peptase feedstuff.
Test example 1
Test products: embodiment 1
Test products research unit: sincere bio tech ltd is won in Deqing, Zhejiang
Test products pilot scale, process unit: Deqing Bo Shijia bio tech ltd pilot scale, production
Experimental field: Jinhua, Zhejiang adds China and plants the pig farm
Test pig kind: be outer ternary breeding pig
Usage: in feed, add 5% embodiment 1, the fish meal of equivalent substitution 2% and 3% dregs of beans.
Test situation and result:
Conclusion:
Test group and control group ratio, have: 1, feed cost is equal to, if the feed average unit cost is 3.00 yuan/kg, every pig can be saved 54 yuan of feed costs (unit).2, have loose bowels and reduce more than 54.4% on year-on-year basis.3, death rate reduces by 63.6%.
Test example 2
Test products: embodiment 2
Test products research unit: sincere bio tech ltd is won in Deqing, Zhejiang
Test products pilot scale, process unit: Deqing Bo Shijia bio tech ltd pilot scale, production
Experimental field: the green resultant field of raising chickens of Zhejiang Long Youkang
Test chicken kind: be three yellow chickens (laying hen)
Usage: in feed, add 5% embodiment 2 products, the fish meal of equivalent substitution 2% and 3% dregs of beans.
Test situation and result:
Conclusion: test group compares with control group: 1, death rate reduce near half.2, have loose bowels and reduce by 67.7% on year-on-year basis.3, laying rate, egg size are all than control group height.4, feedstuff-egg ratio is more lower slightly than control group.
Test example 3
1.1 test soft-shelled turtle
Test is that the intensive culture pond moves to the pool cultivated soft-shelled turtle of soil with soft-shelled turtle.Totally 15 mouthfuls of Tu Chi are divided into five groups, every group of 3 mouthfuls of ponds.Establish control group and four test group, i.e. test group I, II, III and IV respectively.Soft-shelled turtle amounts to 203600 tails, 4040.2 kilograms of weight.Quantity distribution of soft-shelled turtle and specification size etc. see Table 1.
Table 1 is respectively organized soft-shelled turtle and is thrown in situation
1.2 test feed
Test provides prescription with feed by the permanent emerging group in Guangdong and produces, and the fineness of raw material is passed through 80 mesh sieves more than 97%.Raw material mainly adopts Chilean red fish meal, U.S.'s seafood white fish meal and contains the feed etc. of the embodiment 1 of Chinese herbal medicine, forms rotten shape feed through cooperating meticulously.Its prescription sees Table 2, and test group I, II, III and IV are respectively with 3%, 5%, 7%, 9% embodiment 1 described feed equivalent substitution red fish meal.
Each is organized the feed color and luster and is pale yellow powder shape, 97% by 80 mesh sieves, and viscoplasticity was more than 15 minutes does not does not scatter and disappear in water.Each testing result of organizing feed ingredient sees Table 3.
The composition of table 2 test group and control group feed
Table 3 is respectively organized the feed ingredient content of feed
(annotate: VBN is a volatile salts ground state nitrogen)
1.3 each test tank condition of water quality
Test tank is basic identical with the water quality environment in contrast pond, measured once in per 7 days: the depth of water is 1.6-1.8 rice, and the pH value is 7-8.2, water temperature 8-34 ℃, salinity is 0.2-0.3%, ammonia nitrogen value 0.2-0.35ppm, nitrite 0.1-0.2ppm, transparency 10-25cm, algae is based on green alga, dissolved oxygen amount is based on natural dissolved oxygen amount (3-6mg/L), and aerator only starts less at ordinary times use after feeding and during Changes in weather.Pull the line item statistics of going forward side by side when finding dead fish out.
1.4 feeding and management
Tested on December 19,6 days～2010 June in 2010 and carry out, totally six first quarter moons, 199 days by a definite date in 863 proving grounds, south of East Sea Island, Zhanjiang City, Guangdong.
Throwing something and feeding, feed every day, early ((17:00～18:00) totally twice, suitably adjusts the daily ration, feeding quantity and the number of times of throwing something and feeding when sashimi (raw fish) disease or climate change for 5:00～6:00), evening.The feed of eating up is pulled out and is delivered to other fishpond and throw something and feed, and the record statistics.Do not add fish oil or other greases in the feeding process.Regularly pull the dead algae of bubbling through the water column every day out.
1.5 evaluation index
Feed coefficient=daily ration, feeding quantity/net gain weight;
Survival rate=(total supply volume-dead quantity)/total supply volume
Net gain rate * 100=net gain weight/input weight * 100;
Protein efficiency %=body weight gains amount/(total daily ration, feeding quantity bait * bait protein content) * 100
1.6 statistical analysis
Adopt SAS software to carry out variance analysis and significance test.
2, result
2.1 the feed of embodiment 1 is to the influence of soft-shelled turtle growth
After 199 days by a definite date raising, with the soft-shelled turtle hauling of all test group and control group, write down the soft-shelled turtle quantity in every pond, weigh, obtain mantissa's (seeing Table 4) of output and fish from each pond.
From the statistics of table 4 as can be seen: add 3% feed coefficient minimum (1.35) of test group I of feed that contains the embodiment 1 of Chinese herbal medicine, reduced by 6.9% (P＜0.05) than control group; Secondly for the feed coefficient (1.41) of the test group II of the feed that adds 5% embodiment 1 than control group (1.45) lower slightly (P＞0.05); The feed coefficient (1.47) of test group III of feed that adds 7% embodiment 1 is than control group slightly high (P＞0.05).From average net gain rate as can be seen: the average net gain rate of all test group all is higher than control group, wherein add the average net gain rate the highest (3.62) of test group II of the feed of 5% embodiment 1, this explanation interpolation 9% can improve the efficiency of gain of soft-shelled turtle with the red fish meal of the alternative equivalent of feed of interior embodiment 1.
The amount of fishing of table 4 test group and control group and statistics
2.2 the feed of embodiment 1 is to the influence of soft-shelled turtle survival rate, sick rate
As seen, in 9% alternate range, along with the alternative increase of measuring of the feed of embodiment 1, sick rate, the death rate are tangible downward trend in the table 4.
Test example 4
Test products: embodiment 1
Test products research unit: sincere bio tech ltd is won in Deqing, Zhejiang
Test products pilot scale, process unit: Deqing Bo Shijia bio tech ltd pilot scale, production
Experimental field: Zhejiang beacon group.
Conclusion: NH in the pig house environment
3Content on average reduce by 42.4% than control group;
N-NH in the fresh pig dung
4 +The content experimental group on average reduce by 23.3% than control group.
Claims (7)
1. biologically active protein, peptide feed that contains Chinese herbal medicine is characterized in that this feed is made of following component by weight percentage:
Biologically active protein, peptide feedstuff 80%-99%,
Asiatic plantain or Herba Plantaginis extract 0.5%-10%,
Gold grass or the gold careless extract 0.5%-10% that boils that boils.
2. a kind of biologically active protein, peptide feed that contains Chinese herbal medicine according to claim 1 is characterized in that this feed is made of following component by weight percentage:
Biologically active protein, peptide feedstuff 80%-94%,
Asiatic plantain 3%-10%,
The gold careless 3%-10% that boils.
3. a kind of biologically active protein, peptide feed that contains Chinese herbal medicine according to claim 1 is characterized in that this feed is made of following component by weight percentage:
Biologically active protein, peptide feedstuff 90%-99%,
Herba Plantaginis extract 0.5%-5%,
The gold careless extract 0.5%-5% that boils.
4. one kind prepares the described method that contains the biologically active protein, peptide feed of Chinese herbal medicine of claim 1, it is characterized in that this method comprises the steps: 1) take by weighing required Asiatic plantain and the gold grass that boils on request, 2) Asiatic plantain and gold are boiled grass meal is broken to 40-60 order, 3) mix with the biologically active protein, peptide feedstuff.
5. one kind prepares the described method that contains the biologically active protein, peptide feed of Chinese herbal medicine of claim 1, it is characterized in that this method comprises the steps: 1) take by weighing required Asiatic plantain and the gold grass that boils on request, 2) medicinal material is extracted with 70%-95% ethanol water, be concentrated into dried, 3) mix with the biologically active protein, peptide feedstuff.
6. be used for preparing the application of the feed of control livestock and poultry or aquatic livestock diarrhoea, antiviral or breathing problem according to the described biologically active protein, peptide feed that contains Chinese herbal medicine of any claim of claim 1～3.
7. be used for preparing the application of the feed of preventing and treating livestock and poultry or aquatic livestock diarrhoea according to the described biologically active protein, peptide feed that contains Chinese herbal medicine of any claim of claim 1～3.
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| CN201310168329.4A Division CN103271236B (en) | 2011-07-15 | 2011-07-15 | Preparation method of biological active protein peptide feed containing traditional Chinese herb extracts |
| CN201310168310.XA Division CN103262946B (en) | 2011-07-15 | 2011-07-15 | Preparation method of bioactive protein peptide forage containing Chinese herbal medicines |
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Cited By (1)
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| CN104543564A (en) * | 2015-01-09 | 2015-04-29 | 霍邱县兴伟水产养殖专业合作社 | Soft-shelled turtle feed formula and preparation method thereof |
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| CN101357229A (en) * | 2007-08-01 | 2009-02-04 | 方希修 | Production technique of immunoglobulin compounding agent capable of preventing and treating pig diarrhea |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101357229A (en) * | 2007-08-01 | 2009-02-04 | 方希修 | Production technique of immunoglobulin compounding agent capable of preventing and treating pig diarrhea |
Non-Patent Citations (5)
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| 《中国饲料》 20031231 程远芳，宋代军 生物活性肽在饲料工业中的应用 第19-20页 1-7 , 第10期 * |
| 《动物营养学报》 20041231 陈路，张日俊 生物活性肽（或寡肽）饲料添加剂的研究与应用 12-14,21 1-7 第16卷, 第2期 * |
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Cited By (1)
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| CN104543564A (en) * | 2015-01-09 | 2015-04-29 | 霍邱县兴伟水产养殖专业合作社 | Soft-shelled turtle feed formula and preparation method thereof |
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Owner name: ZHEJIANG BOSHIJIA BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: ZHEJIANG DEQING BOCHENG BIO-TECHNOLOGY CO., LTD. Effective date: 20141216 |
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Effective date of registration: 20141216 Address after: 313200 Zhejiang city of Huzhou province Deqing County Yu Yue Zhen Gao Qiao Cun Whitehorse No. 103 Patentee after: Zhejiang Boshijia Biotechnology Co.,Ltd. Address before: 313200 Zhejiang city of Huzhou province Deqing County Wukang Changhong Street 345 4 floor Patentee before: Zhejiang Deqing Bocheng Bio-technology Co.,Ltd. |
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Denomination of invention: A bioactive protein peptide feed containing Chinese herbal medicine and its preparation method and application Effective date of registration: 20230823 Granted publication date: 20130807 Pledgee: Yuyue sub branch of Zhejiang Deqing Rural Commercial Bank Co.,Ltd. Pledgor: Zhejiang Boshijia Biotechnology Co.,Ltd. Registration number: Y2023980053363 |
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