CN102274443A - Compound Chinese medicinal microemulsion preparation for improving immunity - Google Patents
Compound Chinese medicinal microemulsion preparation for improving immunity Download PDFInfo
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- CN102274443A CN102274443A CN2011102290804A CN201110229080A CN102274443A CN 102274443 A CN102274443 A CN 102274443A CN 2011102290804 A CN2011102290804 A CN 2011102290804A CN 201110229080 A CN201110229080 A CN 201110229080A CN 102274443 A CN102274443 A CN 102274443A
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- 239000004530 micro-emulsion Substances 0.000 title claims abstract description 59
- 230000036039 immunity Effects 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title abstract description 13
- 150000001875 compounds Chemical class 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 claims abstract description 66
- 150000004676 glycans Chemical class 0.000 claims abstract description 38
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 38
- 239000005017 polysaccharide Substances 0.000 claims abstract description 38
- 239000004094 surface-active agent Substances 0.000 claims abstract description 26
- 239000004064 cosurfactant Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 15
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims abstract description 14
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 7
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 7
- 241001061264 Astragalus Species 0.000 claims abstract description 6
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims abstract description 6
- 235000006533 astragalus Nutrition 0.000 claims abstract description 6
- 210000004233 talus Anatomy 0.000 claims abstract description 6
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims abstract description 5
- 244000234609 Portulaca oleracea Species 0.000 claims abstract description 5
- 235000001855 Portulaca oleracea Nutrition 0.000 claims abstract description 5
- 235000019437 butane-1,3-diol Nutrition 0.000 claims abstract description 5
- -1 polyoxyethylene Polymers 0.000 claims abstract description 5
- 238000009472 formulation Methods 0.000 claims description 23
- 239000002245 particle Substances 0.000 claims description 12
- ZEMPKEQAKRGZGQ-AAKVHIHISA-N 2,3-bis[[(z)-12-hydroxyoctadec-9-enoyl]oxy]propyl (z)-12-hydroxyoctadec-9-enoate Chemical class CCCCCCC(O)C\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CC(O)CCCCCC)COC(=O)CCCCCCC\C=C/CC(O)CCCCCC ZEMPKEQAKRGZGQ-AAKVHIHISA-N 0.000 claims description 4
- 229940058015 1,3-butylene glycol Drugs 0.000 claims description 3
- 239000009806 pulsatillae Substances 0.000 claims description 3
- 239000003921 oil Substances 0.000 abstract description 19
- 239000003814 drug Substances 0.000 abstract description 12
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 abstract 1
- 235000003097 Artemisia absinthium Nutrition 0.000 abstract 1
- 240000001851 Artemisia dracunculus Species 0.000 abstract 1
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 abstract 1
- 235000003261 Artemisia vulgaris Nutrition 0.000 abstract 1
- 241000206469 Pulsatilla Species 0.000 abstract 1
- 240000001949 Taraxacum officinale Species 0.000 abstract 1
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 abstract 1
- 238000003975 animal breeding Methods 0.000 abstract 1
- 239000001138 artemisia absinthium Substances 0.000 abstract 1
- 239000004359 castor oil Substances 0.000 abstract 1
- 235000019438 castor oil Nutrition 0.000 abstract 1
- 239000010647 garlic oil Substances 0.000 abstract 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 abstract 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 22
- 238000003756 stirring Methods 0.000 description 14
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- 241000287828 Gallus gallus Species 0.000 description 5
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- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 229960001438 immunostimulant agent Drugs 0.000 description 4
- 239000003022 immunostimulating agent Substances 0.000 description 4
- 230000003308 immunostimulating effect Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- 206010059866 Drug resistance Diseases 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
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- 238000002474 experimental method Methods 0.000 description 2
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- 238000010255 intramuscular injection Methods 0.000 description 2
- 239000007927 intramuscular injection Substances 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
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- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- FHNINJWBTRXEBC-UHFFFAOYSA-N Sudan III Chemical compound OC1=CC=C2C=CC=CC2=C1N=NC(C=C1)=CC=C1N=NC1=CC=CC=C1 FHNINJWBTRXEBC-UHFFFAOYSA-N 0.000 description 1
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- 229940088598 enzyme Drugs 0.000 description 1
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- 230000002519 immonomodulatory effect Effects 0.000 description 1
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- 238000009776 industrial production Methods 0.000 description 1
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- 238000003475 lamination Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
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- Medicinal Preparation (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a compound traditional Chinese medicine microemulsion preparation capable of improving immunity, which comprises the following components in parts by weight: 1.8-10% of oil phase, 3.7-33.2% of surfactant, 0-8% of cosurfactant and 51-90% of water phase; the oil phase is a mixture consisting of garlic oil and argy wormwood leaf oil; the surfactant is one or more of tween-80, polyoxyethylene hydrogenated castor oil and span-80; the cosurfactant is one or more of glycerol, ethanol, n-butanol and 1, 3-butanediol; the water phase is a polysaccharide water solution with the mass concentration of 0.5-15%, and the polysaccharide is one or more of purslane polysaccharide, astragalus polysaccharide, dandelion polysaccharide and Chinese pulsatilla polysaccharide; the invention has the advantages of high bioavailability, low cost, simple preparation and the like, and can be widely applied to the animal breeding production process.
Description
Technical field
The present invention relates to a kind of herbal mixture microemulsion formulation, especially a kind of herbal mixture microemulsion formulation that can improve immunity.
Background technology
Along with the development of animal husbandry intensification and scale, healthy aquaculture and food safety have caused people's extensive concern.Chinese herbal feed additive is in modernization, intensive farm are already produced, the product that performance Chinese medicine disease-prevention health, superiority development such as promote grow, have no drug resistance get up is low with its toxicity, side effect is little, many target spots, pathogenic microorganism are difficult for the chemical sproof advantage of generation to it and cause that people more and more pay attention to.The Chinese herbal medicine immunostimulant is being brought into play important effect aspect immunosuppressive disease and the enhancing human body immunity effect.Chinese medicine can strengthen the non-specific and specific immunity of animal body by different model of action, as strengthening the immunogenicity and the stability of macrophage activity, antigenic substance, the synthetic and secretion of enhancing antibody etc.Some can change or the enhancement antigen material non-specificly, and the mechanism of different its immunological enhancements of Chinese medicine ingredients also is not quite similar, and especially highlights on multi-target effect.Therefore, the Chinese herbal medicine immunostimulant will have vast potential for future development in promoting the livestock birds health cultivation process.
On volatile oil immunity pharmacological research, the volatile oil in the Bulbus Allii---garlicin enjoys attention, and extensive use studies confirm that in aquaculture, and garlicin has significant immunological enhancement, can improve the lysozyme content in the serum and the activity of some enzymes.The herbal polysaccharide research experiment proves that polysaccharide has multiple biological activitys such as the immunomodulating of promotion, antitumor, antiviral, radioprotective, defying age, parasiticide, and disease prevention and health care are had tangible promotion and potentiation.Simultaneously, polysaccharide is a natural product, has that toxic and side effects is little, noresidue, does not produce advantages such as drug resistance, thereby have broad application prospects.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of herbal mixture microemulsion formulation that can improve immunity with advantages such as bioavailability height, cost are low, preparation is simple.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
The herbal mixture microemulsion formulation that can improve immunity of the present invention, its weight consists of: oil phase 1.8-10%, surfactant 3.7-33.2%, cosurfactant 0-8%, water 51-90%;
Wherein, the mixture that described oil phase is made up of Oleum Bulbus Allii and Oleum Folium Artemisiae Argyi, wherein Oleum Bulbus Allii is 50-70%, Oleum Folium Artemisiae Argyi is 30-50%;
Described surfactant is a tween 80, polyoxyethylene hydrogenated Oleum Ricini, one or more in the span-80;
Described cosurfactant is one or more in glycerol, ethanol, n-butyl alcohol and the 1,3 butylene glycol;
Described water is that mass concentration is the 0.5-15% polysaccharide solution, and described polysaccharide is one or more in Polysaccharide from Portulaca oleracea, astragalus polysaccharides, Herba Taraxaci polysaccharide and the Radix Pulsatillae polysaccharide;
A kind of preparation method that improves the herbal mixture microemulsion formulation of immunity comprises the steps:
(1) surfactant and cosurfactant are mixed in proportion.
(2) Oleum Bulbus Allii and Oleum Folium Artemisiae Argyi are added in the mixed system of surfactant and cosurfactant, and stir.
(3) under the room temperature, in the mixture of step (2) gained, dropwise add polysaccharide solution while stirring, become sticky by rare until system, again by sticking thinning, the solution clear.
The microemulsion formulation of the present invention's preparation detects through the Ma Erwen Particle Size Analyzer, and particle diameter is at 10-100nm, and the outward appearance clarification is bright, good fluidity, steady quality.Below be by experiment physicochemical property, stability and the immunostimulant performance of microemulsion formulation to be detected:
Testing 1 microemulsion physicochemical property detects
(1) outward appearance of microemulsion
The microemulsion of preparation is pale brown color (being colourless when not adding polysaccharide solution), and clarification is transparent, good fluidity.
(2) form of microemulsion
The herbal mixture microemulsion that takes a morsel is an amount of, routinely the operation transmission electron microscope observation.The result shows that microemulsion great majority under Electronic Speculum are spheroidal.
(3) particle diameter of microemulsion
It is an amount of to get the herbal mixture microemulsion, and with measuring with the Ma Erwen Particle Size Analyzer behind an amount of distilled water diluting, measurement result shows that the mean diameter of the herbal mixture microemulsion of test preparation is 19.41.The coefficient of dispersion is 0.594, and as seen the particle size distribution of this microemulsion formulation is narrower, and size ratio is more even.
(4) type of microemulsion
Utilizing staining is the type that speed that oil-soluble dyes tonyred (redness) and water-soluble dye methylene blue (blueness) spread in microemulsion is judged microemulsion, if red diffusion then is the w/o type microemulsion faster than blueness; Otherwise then be O/W type microemulsion, the result shows that the microemulsion of the present invention's preparation is the O/W type.
Test 2 stability tests
(1) centrifugal acceleration test is to the influence of this traditional medicine volatile oil microemulsion stability
In centrifuge tube, sealing orifice places high speed centrifuge in right amount in the present invention who gets preparation, and with 13,000r/min carries out centrifugal 20min, and this microemulsion still keeps original outward appearance, and clear is not seen lamination.
(2) the light stability test is to the influence of this traditional medicine volatile oil microemulsion stability
An amount of the present invention is prepared the Chinese medicine microemulsion formulation place Clear glass bottles and jars, sealing places under the daylight lamp, and light at room temperature is according to 15d, and in 1d, 3d, 5d, 10d, the 15d observation of taking a sample, the result shows that the present invention at the duration of test apparent colour significant change does not take place, and does not have phenomenons such as layering and breakdown of emulsion.
(3) the temperature stability test is to the influence of this traditional medicine volatile oil microemulsion stability
The present invention of preparation is sub-packed in the vial in right amount, and sealing is placed on to keep sample under 4 ℃, 25 ℃, 37 ℃ and the 60 ℃ of conditions investigates 3 months, and sampling in per 15 days is observed.The result shows that the present invention remains the outward appearance of clear under these four kinds of temperature conditions, do not see tangible layering and demulsifying phenomenon.
Test the test of 3 immunostimulant
Protective effect to chicken colibacillosis
240 of 21 age in days broiler are divided into 4 groups at random, every group of 3 repetitions.Processing mode and result of the test are as follows:
Wherein the blank group after administration the 5th day intramuscular injection normal saline 0.2mL/ only, model group and microemulsion group intramuscular injection escherichia coli O1 bacterial strain 10
8CFU 0.2mL/ only.。
Grouping of table 1 animal and result of the test
| Group | Medication | Dead number of elements | Mortality rate (%) |
| The blank group | —— | 0 | 0 |
| Model control group | —— | 33 | 55 |
| Herbal mixture microemulsion low dose group | 50mL microemulsion+950mL diseases caused by retention of fluid water | 30 | 50 |
| Herbal mixture microemulsion high dose group | 150mL microemulsion+850mL diseases caused by retention of fluid water | 10 | 16.67 |
By experimental result as can be known, the mortality rate of herbal mixture microemulsion high dose group has reduced by 33.33 percentage points than model control group.Herbal mixture microemulsion low dose group mortality rate is suitable with model group.
We also find the difference of a chicken death time in experimentation, result such as table 2:
The death time statistical result of table 2 laboratory animal
| Group | 0h-24h | 24h-36h | 36h-48h | 48h-72h | 72h-96h |
| The blank group | 0 | 0 | 0 | 0 | 0 |
| Model control group | 4 | 6 | 10 | 8 | 5 |
| Herbal mixture microemulsion low dose group | 1 | 2 | 8 | 10 | 9 |
| Herbal mixture microemulsion high dose group | 0 | 1 | 3 | 5 | 1 |
As shown in Table 2, morbidity of herbal mixture microemulsion group chicken and death time are more late than the dead peak of the chicken time of occurrence of model group, and this has just won the time for treatment of diseases.
The prepared herbal mixture microemulsion formulation technology of the present invention is simple, has good stability, and safe in utilization, production cost is low, long shelf-life.Can significantly strengthen disease resistance of animals, can be widely used in the animal cultivation production process.
Adopt the beneficial effect that technique scheme produced to be:
1. the prepared microemulsion bioavailability of the present invention is higher, can reduce the use amount of medicine effectively, and this microemulsion uses traditional medicine volatile oil effect oil phase, does not need to select independent oil phase, greatly reduces the cost of microemulsion preparation.
2. effectively solve Oleum Bulbus Allii and the Oleum Folium Artemisiae Argyi dissolubility is low, the resting period is short, shortcoming such as easily go mouldy.
3. traditional medicine volatile oil and the immiscible problem of polysaccharide solution in the solution practical operation make both perfect adaptations, bring into play dual immunological enhancement clinically.
4. this microemulsion formulation in use can dilute with water, forms the microemulsion of clear, and this microemulsion Thermodynamically stable is easy to preparation and stores.
5. the prescription and the method simple possible of the present invention's employing are convenient to large-scale industrial production.
The specific embodiment
Embodiment 1
1) oil phase: Oleum Bulbus Allii 0.9g, Oleum Folium Artemisiae Argyi 0.9g;
Surfactant: tween 80 10g;
Water: mass concentration is 10% astragalus polysaccharides aqueous solution 88.2g.
2) with surfactant and the cosurfactant mix homogeneously selected for use, oil phase is added in the mixed system of surfactant and cosurfactant then, and stir.
3) under the room temperature, dropwise add polysaccharide solution while stirring in the mixture of step (2) gained, become sticky by rare until system, by sticking thinning, the solution clear promptly gets the herbal mixture microemulsion formulation of particle diameter at 10-100 nm again.
Embodiment 2
1) oil phase: Oleum Bulbus Allii 2g, Oleum Folium Artemisiae Argyi 1g;
Surfactant: tween 80 6g;
Cosurfactant: ethanol 4g, glycerol 3g, n-butyl alcohol 3g;
Water: mass concentration is 0.5% Polysaccharide from Portulaca oleracea aqueous solution 40g, and mass concentration is 2% Herba Taraxaci polysaccharide solution 50g.
2) with surfactant and the cosurfactant mix homogeneously selected for use, oil phase is added in the mixed system of surfactant and cosurfactant then, and stir.
3) under the room temperature, dropwise add polysaccharide solution while stirring in the mixture of step (2) gained, become sticky by rare until system, by sticking thinning, the solution clear promptly gets the herbal mixture microemulsion formulation of particle diameter at 10-100 nm again.
Embodiment 3
1) oil phase: Oleum Bulbus Allii 3g, Oleum Folium Artemisiae Argyi 2g;
Surfactant: polyoxyethylene hydrogenated Oleum Ricini 20g, span-80 10g;
Cosurfactant: 1,3 butylene glycol 4g;
Water: mass concentration is 15% Herba Taraxaci polysaccharide solution 71g.
2) with surfactant and the cosurfactant mix homogeneously selected for use, oil phase is added in the mixed system of surfactant and cosurfactant then, and stir.
3) under the room temperature, dropwise add polysaccharide solution while stirring in the mixture of step (2) gained, become sticky by rare until system, by sticking thinning, the solution clear promptly gets the herbal mixture microemulsion formulation of particle diameter at 10-100 nm again.
Embodiment 4
1) oil phase: Oleum Bulbus Allii 4.2g, Oleum Folium Artemisiae Argyi 2.8g;
Surfactant: tween 80 25g;
Cosurfactant: n-butyl alcohol 8g;
Water: mass concentration is 7% Radix Pulsatillae polysaccharide solution 60g.
2) with surfactant and the cosurfactant mix homogeneously selected for use, oil phase is added in the mixed system of surfactant and cosurfactant then, and stir.
3) under the room temperature, dropwise add polysaccharide solution while stirring in the mixture of step (2) gained, become sticky by rare until system, by sticking thinning, the solution clear promptly gets the herbal mixture microemulsion formulation of particle diameter at 10-100 nm again.
Embodiment 5
1) oil phase: Oleum Bulbus Allii 5g, Oleum Folium Artemisiae Argyi 4g;
Surfactant: span-80 33.2g
Cosurfactant: 1,3 butanediol 5g;
Water: mass concentration is 5% astragalus polysaccharides aqueous solution 30g, and mass concentration is 10% Polysaccharide from Portulaca oleracea aqueous solution 22.8g.
2) with surfactant and the cosurfactant mix homogeneously selected for use, oil phase is added in the mixed system of surfactant and cosurfactant then, and stir.
3) under the room temperature, dropwise add polysaccharide solution while stirring in the mixture of step (2) gained, become sticky by rare until system, by sticking thinning, the solution clear promptly gets the herbal mixture microemulsion formulation of particle diameter at 10-100 nm again.
Embodiment 6
1) oil phase: Oleum Bulbus Allii 7g, Oleum Folium Artemisiae Argyi 3g;
Surfactant: tween 80 10g, polyoxyethylene hydrogenated Oleum Ricini 10g, span-80 12g;
Cosurfactant: glycerol 7g;
Water: mass concentration is 3% astragalus polysaccharides aqueous solution 51g.
2) with surfactant and the cosurfactant mix homogeneously selected for use, oil phase is added in the mixed system of surfactant and cosurfactant then, and stir.
3) under the room temperature, dropwise add polysaccharide solution while stirring in the mixture of step (2) gained, become sticky by rare until system, by sticking thinning, the solution clear promptly gets the herbal mixture microemulsion formulation of particle diameter at 10-100 nm again.
Claims (3)
1. the herbal mixture microemulsion formulation that can improve immunity is characterized in that the weight of described herbal mixture microemulsion formulation consists of: oil phase 1.8-10%, surfactant 3.7-33.2%, cosurfactant 0-8%, water 51-90%;
The mixture that described oil phase is made up of Oleum Bulbus Allii and Oleum Folium Artemisiae Argyi;
Described surfactant is a tween 80, polyoxyethylene hydrogenated Oleum Ricini, one or more in the span-80;
Described cosurfactant is one or more in glycerol, ethanol, n-butyl alcohol and the 1,3 butylene glycol;
Described water is that mass concentration is the 0.5-15% polysaccharide solution, and described polysaccharide is one or more in Polysaccharide from Portulaca oleracea, astragalus polysaccharides, Herba Taraxaci polysaccharide and the Radix Pulsatillae polysaccharide.
2. a kind of herbal mixture microemulsion formulation that can improve immunity according to claim 1 is characterized in that the mass percent of described oil phase consists of: Oleum Bulbus Allii 50-70%, Oleum Folium Artemisiae Argyi 30-50%.
3. a kind of herbal mixture microemulsion formulation that can improve immunity according to claim 1, the particle diameter that it is characterized in that microemulsion formulation is 10-100nm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104921005A (en) * | 2015-03-20 | 2015-09-23 | 徐州黎明食品有限公司 | Garlic oil nanometer-scale microemulsion liquid and preparation method thereof |
| WO2021230894A1 (en) * | 2020-05-15 | 2021-11-18 | Saudi Arabian Oil Company | Enhancing foam stability using allium sativum oil |
| CN117322536A (en) * | 2023-10-31 | 2024-01-02 | 宁夏大洋饲料科技有限公司 | Premixed feed for improving calf organism immunity and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748777A (en) * | 2004-09-15 | 2006-03-22 | 上海医药工业研究院 | Chinese medicine volatoile oil self-mciro emulsifying nano composition and preparing method |
| CN101708314A (en) * | 2009-12-10 | 2010-05-19 | 中国人民解放军第二军医大学 | Chinese medicinal essential oil injection solution, injection and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748777A (en) * | 2004-09-15 | 2006-03-22 | 上海医药工业研究院 | Chinese medicine volatoile oil self-mciro emulsifying nano composition and preparing method |
| CN101708314A (en) * | 2009-12-10 | 2010-05-19 | 中国人民解放军第二军医大学 | Chinese medicinal essential oil injection solution, injection and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104921005A (en) * | 2015-03-20 | 2015-09-23 | 徐州黎明食品有限公司 | Garlic oil nanometer-scale microemulsion liquid and preparation method thereof |
| WO2021230894A1 (en) * | 2020-05-15 | 2021-11-18 | Saudi Arabian Oil Company | Enhancing foam stability using allium sativum oil |
| CN117322536A (en) * | 2023-10-31 | 2024-01-02 | 宁夏大洋饲料科技有限公司 | Premixed feed for improving calf organism immunity and preparation method thereof |
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