CN102253200A - Enzyme-linked immuno sorbent assay (elisa) reader, elisa plate and machining method thereof - Google Patents
Enzyme-linked immuno sorbent assay (elisa) reader, elisa plate and machining method thereof Download PDFInfo
- Publication number
- CN102253200A CN102253200A CN2011101770611A CN201110177061A CN102253200A CN 102253200 A CN102253200 A CN 102253200A CN 2011101770611 A CN2011101770611 A CN 2011101770611A CN 201110177061 A CN201110177061 A CN 201110177061A CN 102253200 A CN102253200 A CN 102253200A
- Authority
- CN
- China
- Prior art keywords
- temperature sensor
- elisa plate
- temperature
- ds18b20
- elisa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Measuring And Recording Apparatus For Diagnosis (AREA)
- Measuring Temperature Or Quantity Of Heat (AREA)
Abstract
The invention discloses an enzyme-linked immuno sorbent assay (elisa) reader, an elisa plate and a machining method thereof, which aim to realize the temperature control of an ELISA experiment. In the elisa reader, a temperature sensor is arranged on the elisa plate and is electrically connected with a temperature routing inspection meter. The elisa plate is provided with the temperature sensor. The machining method comprises the following steps of: taking the elisa plate; punching at the position where which the temperature sensor is needed to be inserted; injecting paraffin oil into a hole; and arranging a printed circuit board (PCB) with the temperature sensor on the elisa plate, and inserting the temperature sensor into the hole into which the paraffin oil is injected.
Description
Technical field
The present invention relates to a kind of testing tool, relate in particular to a kind of microplate reader, ELISA Plate and job operation thereof of carrying out enzymatic activity and immunoassay.
Background technology
Microplate reader (Micro plate Reader) is the professional instrument that enzyme linked immunosorbent detection (EIA) experimental result is read and analyzes, and its core is a tintmeter, promptly analyzes the content of antigen or antibody with colourimetry.Enzymatic chromogenic substrate on antigen or the antibody carries out enzyme linked immunoassay by being coupled at, and reaction result shows with color, and the depth by colour developing is the concentration that the size of absorbance just can be judged antibody to be measured in the sample or antigen.
Enzyme linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA) in, temperature controlled accuracy directly has influence on the accuracy that enzymatic activity and immunoassay result measure.Therefore, temperature how accurately to control microplate reader becomes a problem demanding prompt solution.
Summary of the invention
Fundamental purpose of the present invention is to disclose a kind of microplate reader, ELISA Plate and job operation thereof, to realize the temperature control to the ELISA experiment.
For reaching above-mentioned purpose, the present invention discloses a kind of microplate reader, and its ELISA Plate is provided with temperature sensor, and this temperature sensor and temperature polling instrument electrically connect.Wherein ELISA Plate is the carrier of interior adsorption antigen of microplate reader or antibody.
According to microplate reader of the present invention, the ELISA Plate that will have temperature sensor is placed in the microplate reader, close door, the extension line of temperature sensor is connected with temperature polling instrument, open the microplate reader power supply, set the temperature of microplate reader and wait for the stable of each temperature sensor reading, can obtain microplate reader in each regional temperature control situation of ELISA Plate, and then realized accurately controlling each regional temperature of this ELISA Plate, thereby also just can realize the temperature of ELISA experiment is controlled by the adjusting of microplate reader temperature.
For reaching above-mentioned purpose, the present invention also discloses a kind of ELISA Plate, and it is provided with temperature sensor.
For reaching above-mentioned purpose, the present invention also discloses a kind of job operation of ELISA Plate, comprising:
Get ELISA Plate;
Will insert the position punching of temperature sensor;
In the hole, inject paraffin oil;
The pcb board that will have temperature sensor is placed on the ELISA Plate, and temperature sensor is inserted the above-mentioned hole of injecting paraffin oil.
ELISA Plate disclosed in this invention and job operation thereof, simple, convenient, making and raw materials cost are all lower.Preferable, the present invention can measure each regional temperature control situation of ELISA Plate by an array of temperature sensor, and is preferable, can adopt the DS18B20 temperature sensor of single wire digital to form an array of temperature sensor; For example, anyhow respectively three arrange formation one array of temperature sensor with 9 DS18B20 temperature sensors, and with the in parallel all DS18B20 temperature sensors of same data bus, the extension line of described data bus is 3, is connected with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20 respectively.So, on the one hand, adopt the DS18B20 digital temperature sensor of unibus, member is simple, and temperature resolution can reach 0.0625 degree; On the other hand, adopt the digital sensing technology, overcome of the influence of situations such as wiring the temperature measuring accuracy; Moreover the extension line that is arranged so that array of temperature sensor of unibus has only 3, make microplate reader can closely close door in the mensuration process, has guaranteed the consistent of condition determination and actual service conditions.In addition, in this array of temperature sensor, can increase or reduce the quantity of temperature sensor as required, extendability is strong.
Description of drawings
Fig. 1 is the structural representation of the disclosed microplate reader of the embodiment of the invention;
Fig. 2 is the wiring diagram of the disclosed temperature sensing array of the embodiment of the invention;
Figure 3 shows that the flow process chart of the disclosed ELISA Plate of the embodiment of the invention.
Embodiment
Below in conjunction with Figure of description specific embodiments of the invention are done detailed description.
Embodiment one
Present embodiment discloses a kind of microplate reader, and as shown in Figure 1, the ELISA Plate 2 in this microplate reader 1 is provided with temperature sensor 3, and wherein the temperature polling instrument outside this temperature sensor 3 and this microplate reader 14 electrically connects.
Wherein temperature polling instrument 4 is applicable to that multimetering shows and the control temperature, generally can patrol and examine 1～64 drive test amount temperature signal, can go the rounds to detect and show the multi way temperature signal, is used with all kinds of temperature sensors, transmitter.It should be noted that: in other embodiments, this temperature polling instrument 4 also can be integrated into a functional module of this microplate reader 1 even this ELISA Plate 2, and this kind conversion also belongs to protection scope of the present invention.In the present embodiment, temperature polling instrument 4 is placed outside the microplate reader, and its advantage is that this microplate reader only need be improved the employed ELISA Plate of existing microplate reader can reach purpose of the present invention, and when temperature polling instrument 4 or microplate reader go wrong, also be convenient to safeguard and handle.
Because the panel of ELISA Plate has certain area, its hole count can be divided into 48 holes, 96 holes, generally commonly used is 96 holes, therefore single temperature sensor 1 is not enough to feed back complete these ELISA Plate 3 each regional temperature usually, comes ELISA Plate central authorities and each peripheral zone are carried out temperature detection and be necessary to be provided with an array of temperature sensor.For this reason, the present invention is an example with the ELISA Plate in 96 holes, detects ELISA Plate central authorities and each peripheral regional temperature with as shown in Figure 2 array of temperature sensor.
As shown in Figure 2, in the figure, this array of temperature sensor is formed in parallel by 9 DS18B20 temperature sensors 41～49.In pcb board shown in Figure 2,9 DS18B20 temperature sensors 41～49 that correspondence is distributed on the ELISA Plate form an array of temperature sensor, the center of the corresponding ELISA Plate of its central temperature sensor with each three row anyhow; All DS18B20 temperature sensors are connected in parallel on same the data bus, and the extension line of data bus is 3, are connected with GND (ground connection) pin, VDD (supply voltage) pin and DQ (forms data bus) pin of the temperature sensor of each DS18B20.
In Fig. 2, this pcb board also is provided with single 3 needle sockets to connect this extension line and internal temperature sensor array.Preferable, these single 3 needle sockets are located at the central part on this narrow limit of ELISA Plate; This ELISA Plate is provided with the jack that inserts temperature sensor (if the position in the hole that carries on the existing ELISA Plate and size conform to size with the position of the temperature sensor of insertion in advance, then can utilize the hole that this microplate reader carries and omit the step of punching).
In the present embodiment, the DS18B20 temperature sensor of employing has following characteristics:
The temperature value that reflects device with the form of 9 bit digital quantity;
Unique one-wire interface only needs a port pinout to carry out communication (the DS18B20 temperature sensor sends by an one-wire interface or receives information, and therefore only needs a connecting line (adding ground wire) between central microprocessor and DS1820);
The power supply that is used to read and write with temperature transition can obtain from data line itself, need not external power source;
Be convenient to the multiple spot distribution applications; Because each DS1820 has the sheet sequence number of a uniqueness,, so just can be placed on many different places to temperature sensor so many DS1820 can be connected in parallel on the single bus simultaneously;
Temperature-measuring range-55 ℃-+125 ℃ increases progressively with 0.1 ℃;
Temperature digital amount 200ms switching time;
The definable non-volatile temperature alarming setting of user;
Identification of warning search command and sign surpass program limiting temperature (temperature alarming condition).
Based on These characteristics, make ELISA Plate shown in Figure 2 and array of temperature sensor thereof have at least and following advantage:
On the one hand, high precision, high reliability, the DS18B20 digital temperature sensor of employing unibus, member is simple, and temperature resolution can reach 0.0625 degree;
On the other hand, adopt the digital sensing technology, overcome of the influence of situations such as wiring the temperature measuring accuracy;
Moreover the extension line that is arranged so that array of temperature sensor of unibus has only 3, make microplate reader can closely close door in the mensuration process, has guaranteed the consistent of condition determination and actual service conditions.
In addition, in this array of temperature sensor, can increase or reduce the quantity of temperature sensor as required, extendability is strong.
It should be noted that in other embodiments above-mentioned DS18B20 temperature sensor also can be substituted by other 1-wire digital temperature sensor, for example other device in the DS18Bxx series.
Embodiment two
Corresponding with the foregoing description one, present embodiment discloses a kind of job operation of ELISA Plate, as shown in Figure 3, may further comprise the steps:
Step S1, get ELISA Plate.
Step S2, will insert the position punching of temperature sensor.Usually, if carry porosely on the ELISA Plate among the step S1, and the position in this hole conforms to the position of the temperature sensor of pre-insertion, then omits the step of this punching.
Step S3, in the hole, inject paraffin oil.Paraffin oil claims mineral oil again, is the potpourri from the resulting colorless and odorless of crude oil fractionation, has good closure.
Step S4, the pcb board that will have a temperature sensor are placed on the ELISA Plate, and temperature sensor are inserted the above-mentioned hole of injecting paraffin oil.
Wherein, pairing with Fig. 2, the circuit production of this pcb board comprises: respectively three arrange formation one array of temperature sensor anyhow with 9 DS18B20 temperature sensors; With the in parallel all DS18B20 temperature sensors of same data bus, the extension line of data bus is 3, is connected with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20 respectively.Preferable, this pcb board also is provided with single 3 needle sockets to connect this extension line and internal temperature sensor array.When above-mentioned pcb board and ELISA Plate are carried out contraposition, these single 3 needle sockets are located at the central part on this narrow limit of ELISA Plate, the center of the temperature sensor of these array of temperature sensor central authorities being inserted described ELISA Plate.
The job operation of the disclosed ELISA Plate of present embodiment, processing is simple, convenient, and cost is low.
It should be noted that at last: above embodiment only in order to technical scheme of the present invention to be described, is not intended to limit; Although with reference to previous embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that: it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (10)
1. an ELISA Plate is characterized in that, is provided with temperature sensor.
2. ELISA Plate according to claim 1 is characterized in that, described temperature sensor is a 1-wire digital temperature sensor.
3. ELISA Plate according to claim 2 is characterized in that, described 1-wire digital temperature sensor is the DS18B20 temperature sensor.
4. ELISA Plate according to claim 3, it is characterized in that described ELISA Plate is rectangular, 9 described DS18B20 temperature sensors are distributed on the described ELISA Plate, respectively three to arrange formation one array of temperature sensor anyhow, its central temperature sensor inserts the center of described ELISA Plate; And
All DS18B20 temperature sensors are connected in parallel on same the data bus, and the extension line of described data bus is 3, are connected with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20 respectively.
5. ELISA Plate according to claim 4 is characterized in that, described pcb board is provided with single 3 needle sockets and pegs graft to connect temperature sensing array and the temperature polling instrument that places outside the described ELISA Plate for described 3 extension lines; These single 3 needle socket correspondences are located at the central part on the narrow limit of described ELISA Plate.
6. ELISA Plate according to claim 1 is characterized in that, described ELISA Plate is provided with the jack that inserts described temperature sensor.
7. a microplate reader is characterized in that, its ELISA Plate is provided with temperature sensor, and this temperature sensor and temperature polling instrument electrically connect.
8. microplate reader according to claim 7 is characterized in that, described temperature polling instrument places outside the described microplate reader;
Wherein said ELISA Plate is rectangular, described temperature sensor is the DS18B20 temperature sensor, 9 described DS18B20 temperature sensors are distributed on the described ELISA Plate, and respectively three to arrange formation one array of temperature sensor anyhow, its central temperature sensor inserts the center of described ELISA Plate; And all DS18B20 temperature sensors are connected in parallel on same the data bus, and the extension line of described data bus is 3, are connected with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20 respectively.
9. the job operation of an ELISA Plate is characterized in that, comprising:
Get ELISA Plate;
Will insert the position punching of temperature sensor;
In the hole, inject paraffin oil;
The pcb board that will have temperature sensor is placed on the ELISA Plate, and temperature sensor is inserted the above-mentioned hole of injecting paraffin oil.
10. the job operation of ELISA Plate according to claim 9 is characterized in that, the circuit production of described pcb board comprises:
Anyhow respectively three arrange formation one array of temperature sensor with 9 DS18B20 temperature sensors;
With the in parallel all DS18B20 temperature sensors of same data bus, the extension line of described data bus is 3, is connected with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20 respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110177061 CN102253200B (en) | 2011-06-28 | 2011-06-28 | Enzyme-linked immuno sorbent assay (elisa) reader, elisa plate and machining method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110177061 CN102253200B (en) | 2011-06-28 | 2011-06-28 | Enzyme-linked immuno sorbent assay (elisa) reader, elisa plate and machining method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102253200A true CN102253200A (en) | 2011-11-23 |
| CN102253200B CN102253200B (en) | 2013-12-25 |
Family
ID=44980578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110177061 Active CN102253200B (en) | 2011-06-28 | 2011-06-28 | Enzyme-linked immuno sorbent assay (elisa) reader, elisa plate and machining method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102253200B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104729740A (en) * | 2015-02-26 | 2015-06-24 | 中国计量科学研究院 | Multi-channel wireless temperature measuring instrument and measurement method |
| CN109622077A (en) * | 2018-12-10 | 2019-04-16 | 郑州安图生物工程股份有限公司 | Self-enclosed reaction orifice plate and preparation method thereof when a kind of incubation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2837841Y (en) * | 2005-10-31 | 2006-11-15 | 陕西科技大学 | Detector for quick detection of milk sample |
| CN101145060A (en) * | 2007-09-21 | 2008-03-19 | 北京工业大学 | Temperature-controlled array for micro-fluidic chip |
| CN201097243Y (en) * | 2007-09-21 | 2008-08-06 | 北京工业大学 | Temperature control array for mini-flow control chip |
| US20090110024A1 (en) * | 2007-10-25 | 2009-04-30 | Kenji Kamei | Temperature computing instrument and method for calibrating temperature of sensor part used therefor |
| CN101551277A (en) * | 2009-05-18 | 2009-10-07 | 胡业林 | Array type multipoint temperature monitoring system |
| CN202159061U (en) * | 2011-06-28 | 2012-03-07 | 中国计量科学研究院 | Microplate reader and enzyme labeled plate |
-
2011
- 2011-06-28 CN CN 201110177061 patent/CN102253200B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2837841Y (en) * | 2005-10-31 | 2006-11-15 | 陕西科技大学 | Detector for quick detection of milk sample |
| CN101145060A (en) * | 2007-09-21 | 2008-03-19 | 北京工业大学 | Temperature-controlled array for micro-fluidic chip |
| CN201097243Y (en) * | 2007-09-21 | 2008-08-06 | 北京工业大学 | Temperature control array for mini-flow control chip |
| US20090110024A1 (en) * | 2007-10-25 | 2009-04-30 | Kenji Kamei | Temperature computing instrument and method for calibrating temperature of sensor part used therefor |
| CN101551277A (en) * | 2009-05-18 | 2009-10-07 | 胡业林 | Array type multipoint temperature monitoring system |
| CN202159061U (en) * | 2011-06-28 | 2012-03-07 | 中国计量科学研究院 | Microplate reader and enzyme labeled plate |
Non-Patent Citations (2)
| Title |
|---|
| 金懿等: "基于单总线数字温度传感器的分布式测温***", 《暨南大学学报》 * |
| 陈丹等: "基于单总线数字温度传感器的温控***", 《漳州师范学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104729740A (en) * | 2015-02-26 | 2015-06-24 | 中国计量科学研究院 | Multi-channel wireless temperature measuring instrument and measurement method |
| CN109622077A (en) * | 2018-12-10 | 2019-04-16 | 郑州安图生物工程股份有限公司 | Self-enclosed reaction orifice plate and preparation method thereof when a kind of incubation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102253200B (en) | 2013-12-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8869613B2 (en) | Sensor end module, sensor and measuring system | |
| US8639467B2 (en) | Measuring system for determining a value of a physical or chemical, measured variable of a medium and method for operation of the measuring system | |
| CN202159061U (en) | Microplate reader and enzyme labeled plate | |
| CN102253200B (en) | Enzyme-linked immuno sorbent assay (elisa) reader, elisa plate and machining method thereof | |
| CN102478940A (en) | Control circuit for computer system multiplexing pins | |
| CN206311540U (en) | A kind of VOC gas Intelligent Measurement instrument apparatus based on STM32 single-chip microcomputers | |
| CN102201260B (en) | Apparatus and method for communicating parameter of inverter | |
| CN102152642B (en) | Device and method for testing ink box chip of printer | |
| CN200986566Y (en) | Universal device for testing combined circuit board | |
| US20140077814A1 (en) | Method and system for testing matrices and method for controlling voltage clocks | |
| US8499107B2 (en) | Field device for process automation | |
| US11235331B2 (en) | Functionally versatile cassettes | |
| CN202471710U (en) | Quantitative detection electronic pen for disposal in-vitro diagnostic reagent | |
| CN101329379A (en) | Method and device for detecting electronic component pin | |
| CN200986482Y (en) | Air pressure sensor | |
| CN201374194Y (en) | Memory pin signal identification card | |
| CN101738218A (en) | Measuring data processing system and processing method thereof | |
| CN204924262U (en) | Digital changer instrument that shows of LED light beam | |
| CN110456203A (en) | Fingerprint module detecting device and fingerprint mould group detection method | |
| CN218180127U (en) | Time-sharing temperature detection circuit and multilayer soil moisture content detector | |
| CN103267774A (en) | Pipe thermal conductivity tester and test method | |
| CN102539144A (en) | Detection system for assembling automobile gear shifting device | |
| CN201397282Y (en) | Double-color LED indication circuit board for full-automatic blood rheometer | |
| CN103048958A (en) | Fundamental framework of integrated system of virtual electronic measuring instrument | |
| Samotaev et al. | Digital intellectual sensors for gas analysis system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |