CN102242093A - Comprehensive development method of high value-added active components of eggs - Google Patents
Comprehensive development method of high value-added active components of eggs Download PDFInfo
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Abstract
The invention discloses a comprehensive development method of high value-added active components of eggs. The method comprises the following steps of 1) washing up eggs and separating main components of eggshell, egg white and egg yolk, 2) drying and crushing the eggshells into eggshell powder, 3) adding water into the egg white for dilution, carrying out an ion exchange process by an acrylic acid type macroporous cation exchange resin, and carrying out spray drying process on residual liquid to obtain lysozyme-free egg white powder, 4) adding 10% of ammonium sulfate solution into the resin for elution and carrying out an ultrafiltration process and a spray drying process on eluate to obtain lysozyme powder, 5) adding NaCl solution into the egg yolk for dilution and carrying out a centrifugation process to obtain egg yolk clear liquid and egg yolk solids, 6) carrying out the processes of salting out, ultrafiltration and spray drying on the egg yolk clear liquid by three times to obtain yolk antibody crude products, 7) adding absolute ethyl alcohol into the egg yolk solids, carrying out a dissolution process with ultrasonic assistance and filtering to obtain egg yolk oil, 8) carrying out an underpressure distillation process on filtrate obtained form the step 7, adding acetone into the distilled filtrate, and filtering and drying the filtrate containing acetone to obtain phosphatidylcholine bilayer, and 9) carrying out an underpressure distillation and concentration process on filtrate obtained from the step 8 and drying the concentrated filtrate by an oven to obtain egg yolk cholesterol. The comprehensive development method of high value-added active components of eggs has the advantages of reasonable and simple processes, high activity of multiple value-added products, full utilization of active ingredients of eggs, and good feasibility of an industrialization production.
Description
Technical field
The present invention relates to a kind of high added value egg active composition comprehensive exploitation method, belong to active skull cap components industrialization extractive technique field.
Background technology
In recent years, both at home and abroad the deep processing of egg-products is more and more paid attention to.Many developed countries drop into a large amount of fund and science and technology strengths and research and develop, product is except that elementary egg-products such as traditional protein powder, yolk powder, whole egg powder, the high added value natural product has appearred, as more than 60 kinds such as N,O-Diacetylmuramidase, Ovum Gallus domesticus Flavus lecithin, Yolk immune globulins.
Egg is one of human best source of nutrition, contains protein, lipid, microorganism and the mineral substance of a large amount of high biological values, is a huge treasure-house therefore.The albumen of egg is mainly Protalbinic acid and ovoglobulin, contains the N,O-Diacetylmuramidase of 3.4%-3.5% simultaneously.N,O-Diacetylmuramidase is a kind of natural natural, nontoxic, safe zymoprotein, can be used as food preservatives in foodstuffs industry, has anti-infective, antibacterium, antiviral effect clinically at medicine, can be used for dissolving the somatic cells wall on biotechnology.Contain abundant Yelkin TTS, cholesterol, Oil of egg yolk and LECITHIN in the yolk.Yelkin TTS is a kind of naturally occurring emulsifying agent, is widely used in industry such as food, makeup, medicine, weaving, leather.Triglyceride level in the Oil of egg yolk is human important nutritive substance, and linolic acid wherein, linolenic acid, arachidonic acid etc. are indispensable lipid acid in the nutrition.Shell membrane claims Membrana Follicularis ovi again in the eggshell, and the effect of moistening lung, cough-relieving, hemostasis is arranged, and eggshell itself also is the high material of calcium content, can be used for feed and industrial chemicals.
China is the world maximum country of birds, beasts and eggs output, and the resource of enriching birds, beasts and eggs is arranged, but because fowls egg products processing is started late, and high added value natural product high efficiency extraction technology of preparing and product development technology are immature in the birds, beasts and eggs, its industrialization level is lower.Therefore, the efficient development technique of high added value natural product is present very exigence in the further investigation birds, beasts and eggs product.
From egg, extract patent and document single or several prods and occurred much, but these preparation methods often only consider the yield of single product, and other effective constituents in the egg are given up.The present invention has developed a kind of method that fully utilizes egg, gives full play to the value of egg, by rational processing method, obtains N,O-Diacetylmuramidase, Yelkin TTS, yolk antibody, cholesterol and the Oil of egg yolk of high added value, and by product egg-white powder and egg-shell meal.
Summary of the invention
The purpose of this invention is to provide a kind of high added value egg active composition comprehensive exploitation method, product comprises N,O-Diacetylmuramidase, Yelkin TTS, yolk antibody, cholesterol, Oil of egg yolk, egg-white powder and egg-shell meal.
A kind of high added value egg active composition comprehensive exploitation method, its step is as follows:
1) separation of egg main ingredient
A cleans egg, opens, and takes out egg white and yolk, obtains Ovum crusta Gallus domesticus.
B utilizes the egg separator that egg white is separated with yolk, obtains egg white and yolk.
C adds the NaCl solution dilution of 0.1M with yolk according to 1: 2～1: 4 volume ratio, stirs, and the pH that regulates diluent with HCl is 5.2, and the limit adds the HCl limit stirs, 4 ℃ of standing over night, and 8000rpm is centrifugal, obtains yolk clear liquid and egg yolk solid thing.
2) Ovum crusta Gallus domesticus is dried down at 60 ℃, be crushed to 300 orders with pulverizer then, obtain being used for the egg-shell meal of animal-feed.
3) extraction of N,O-Diacetylmuramidase and egg-white powder and preparation
A with the volume ratio thin up of egg white according to 1: 0.5～1: 2, stirs, and stirring velocity is advisable with non-foaming.
B transfers pH to 6～7 with above-mentioned egg white diluent with HCl, waits to do the sample liquid of ion-exchange.
C carries out pre-treatment with 0.1M HCl, 0.1M NaOH respectively with acrylic type macropore weak cation exchange resin, is exchanged into the Na type at last.
D mixed by sample liquid and acrylic type macropore weak cation exchange resin mass ratio in 5: 1～3: 1, stirred 2h under the room temperature, filtered.Collect filtrate, spray-drier carries out drying, obtains removing the egg-white powder of the white powder of N,O-Diacetylmuramidase.
E with the washed with de-ionized water of pH6～7 3 times, removes impurity and foreign protein with resin.
F presses the isopyknic amount of resin, adds 10% ammoniumsulphate soln, slowly stirs 0.5h under the room temperature, filters resin, gets elutriant.Clean resin 3 times, and 3 elutriants are merged.
G enters the ultrafilter ultrafiltration with the elutriant after merging, and carries out desalination and concentrated.
H, the liquid after the ultrafiltration advance people's spray-drier and carry out drying, obtain the N,O-Diacetylmuramidase of white powder.The lytic enzyme activity is at 12000～20000U/mg.
4) extraction of yolk antibody
A with the yolk clear liquid that obtains among the step 1) c, adds ammonium sulfate, is made into 40% saturation concentration, and transfers pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.
B adds precipitation in the entry and to dissolve, and the ratio of the consumption of water and former yolk clear liquid is 1: 2～1: 3.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 32% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.
C adds precipitation in the entry and to dissolve, and the ratio of the consumption of water and former yolk clear liquid is 1: 5～1: 6.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 30% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.
D adds precipitation in the entry and to dissolve, and the ratio of the consumption of water and former yolk clear liquid is 1: 8～1: 10.Solution enters the ultrafilter ultrafiltration, carries out desalination and concentrated.
E, the liquid after the ultrafiltration advance people's spray-drier and carry out drying, obtain the slightly yolk antibody of yellow powder shape of white, and purity is 60%～80%
5) extraction of Yelkin TTS, cholesterol and Oil of egg yolk
A adds 35～45 ℃ dehydrated alcohol with the egg yolk solid thing that obtains among the step 1) c according to 1: 12～1: 16 mass ratio, and it is ultrasonic while stirring to put into ultrasonic apparatus, and ultrasonic frequency is 80～150Hz, and the time is 0.5～1h.
B, non-shock chilling to 20～25 ℃, 300～500 order filtered through gauze, the solids that obtains is for containing a small amount of proteinic Oil of egg yolk.
C, filtrate is carried out underpressure distillation, is concentrated to 1/6～1/8 of original volume, adds 2～3 times acetone, stir, 300～500 order filtered through gauze, solids is dried in 45 ℃ of baking ovens, obtains Yelkin TTS.
D, above-mentioned filtrate is carried out underpressure distillation and is concentrated, and dries in 45 ℃ of baking ovens, obtains yolk cholesterol.
Description of drawings
Fig. 1, the process route chart of high added value egg active composition comprehensive exploitation method.
Embodiment
The invention will be further described by the following examples:
Example 1
Egg is cleaned, opened, take out egg white and yolk, obtain Ovum crusta Gallus domesticus.Ovum crusta Gallus domesticus 60 ℃ of oven dry down, is crushed to 300 orders with pulverizer then, obtains being used for the egg-shell meal of animal-feed.
Utilize the egg separator that egg white is separated with yolk, obtain egg white and yolk.
With the volume ratio thin up of egg white according to 1: 1, stir, stirring velocity is advisable with non-foaming.Above-mentioned egg white diluent is transferred pH to 6 with HCl, wait to do the sample liquid of ion-exchange.Acrylic type macropore weak cation exchange resin is carried out pre-treatment with 0.1M HCl, 0.1M NaOH respectively, be exchanged into the Na type at last.Mix at 3: 1 by sample liquid and acrylic type macropore weak cation exchange resin mass ratio, stir 2h under the room temperature, filter.Collect filtrate, spray-drier carries out drying, obtains removing the egg-white powder of the white powder of N,O-Diacetylmuramidase.Resin with the washed with de-ionized water of pH 63 times, is removed impurity and foreign protein.Press the isopyknic amount of resin, add 10% ammoniumsulphate soln, slowly stir 0.5h under the room temperature, filter resin, get elutriant.Clean resin 3 times, and 3 elutriants are merged.Elutriant after merging is entered the ultrafilter ultrafiltration, carry out desalination and concentrated.Liquid after the ultrafiltration advances people's spray-drier and carries out drying, obtains the N,O-Diacetylmuramidase of white powder.
Yolk is added the NaCl solution dilution of 0.1M according to 1: 3 volume ratio, stir, the pH that regulates diluent with HCl is 5.2, and the limit adds the HCl limit stirs, 4 ℃ of standing over night, and 8000rpm is centrifugal, obtains yolk clear liquid and egg yolk solid thing.
In the yolk clear liquid, add ammonium sulfate, be made into 40% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 2.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 32% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 5.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 30% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 8.Solution enters the ultrafilter ultrafiltration, carries out desalination and concentrated.Liquid after the ultrafiltration advances people's spray-drier and carries out drying, obtains the slightly yolk antibody of yellow powder shape of white.
The egg yolk solid thing is added 45 ℃ dehydrated alcohol according to 1: 14 mass ratio, and it is ultrasonic while stirring to put into ultrasonic apparatus, and ultrasonic frequency is 120Hz, and the time is 1h.Non-shock chilling to 20 ℃, 300～500 order filtered through gauze, the solids that obtains is for containing a small amount of proteinic Oil of egg yolk.Filtrate is carried out underpressure distillation, is concentrated to 1/6 of original volume, adds 2 times acetone, stir, and 300～500 order filtered through gauze, solids is dried in 45 ℃ of baking ovens, obtains Yelkin TTS.Filtrate is carried out underpressure distillation and is concentrated, and dries in 45 ℃ of baking ovens, obtains yolk cholesterol.
Example 2
Egg is cleaned, opened, take out egg white and yolk, obtain Ovum crusta Gallus domesticus.Ovum crusta Gallus domesticus 60 ℃ of oven dry down, is crushed to 300 orders with pulverizer then, obtains being used for the egg-shell meal of animal-feed.
Utilize the egg separator that egg white is separated with yolk, obtain egg white and yolk.
With the volume ratio thin up of egg white according to 1: 1.5, stir, stirring velocity is advisable with non-foaming.Above-mentioned egg white diluent is transferred pH to 6.5 with HCl, wait to do the sample liquid of ion-exchange.Acrylic type macropore weak cation exchange resin is carried out pre-treatment with 0.1M HCl, 0.1M NaOH respectively, be exchanged into the Na type at last.Mix at 4: 1 by sample liquid and acrylic type macropore weak cation exchange resin mass ratio, stir 2h under the room temperature, filter.Collect filtrate, spray-drier carries out drying, obtains removing the egg-white powder of the white powder of N,O-Diacetylmuramidase.Resin with the washed with de-ionized water of pH6.5 3 times, is removed impurity and foreign protein.Press the isopyknic amount of resin, add 10% ammoniumsulphate soln, slowly stir 0.5h under the room temperature, filter resin, get elutriant.Clean resin 3 times, and 3 elutriants are merged.Elutriant after merging is entered the ultrafilter ultrafiltration, carry out desalination and concentrated.Liquid after the ultrafiltration advances people's spray-drier and carries out drying, obtains the N,O-Diacetylmuramidase of white powder.
Yolk is added the NaCl solution dilution of 0.1M according to 1: 2 volume ratio, stir, the pH that regulates diluent with HCl is 5.2, and the limit adds the HCl limit stirs, 4 ℃ of standing over night, and 8000rpm is centrifugal, obtains yolk clear liquid and egg yolk solid thing.
In the yolk clear liquid, add ammonium sulfate, be made into 40% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 2.5.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 32% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 6.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 30% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 10.Solution enters the ultrafilter ultrafiltration, carries out desalination and concentrated.Liquid after the ultrafiltration advances people's spray-drier and carries out drying, obtains the slightly yolk antibody of yellow powder shape of white.
The egg yolk solid thing is added 35 ℃ dehydrated alcohol according to 1: 16 mass ratio, and it is ultrasonic while stirring to put into ultrasonic apparatus, and ultrasonic frequency is 150Hz, and the time is 1h.Non-shock chilling to 20 ℃, 300～500 order filtered through gauze, the solids that obtains is for containing a small amount of proteinic Oil of egg yolk.Filtrate is carried out underpressure distillation, is concentrated to 1/7 of original volume, adds 3 times acetone, stir, and 300～500 order filtered through gauze, solids is dried in 45 ℃ of baking ovens, obtains Yelkin TTS.Filtrate is carried out underpressure distillation and is concentrated, and dries in 45 ℃ of baking ovens, obtains yolk cholesterol.
Example 3
Egg is cleaned, opened, take out egg white and yolk, obtain Ovum crusta Gallus domesticus.Ovum crusta Gallus domesticus 60 ℃ of oven dry down, is crushed to 300 orders with pulverizer then, obtains being used for the egg-shell meal of animal-feed.
Utilize the egg separator that egg white is separated with yolk, obtain egg white and yolk.
With the volume ratio thin up of egg white according to 1: 0.5, stir, stirring velocity is advisable with non-foaming.Above-mentioned egg white diluent is transferred pH to 6 with HCl, wait to do the sample liquid of ion-exchange.Acrylic type macropore weak cation exchange resin is carried out pre-treatment with 0.1M HCl, 0.1MNaOH respectively, be exchanged into the Na type at last.Mix at 5: 1 by sample liquid and acrylic type macropore weak cation exchange resin mass ratio, stir 2h under the room temperature, filter.Collect filtrate, spray-drier carries out drying, obtains removing the egg-white powder of the white powder of N,O-Diacetylmuramidase.Resin with the washed with de-ionized water of pH6 3 times, is removed impurity and foreign protein.Press the isopyknic amount of resin, add 10% ammoniumsulphate soln, slowly stir 0.5h under the room temperature, filter resin, get elutriant.Clean resin 3 times, and 3 elutriants are merged.Elutriant after merging is entered the ultrafilter ultrafiltration, carry out desalination and concentrated.Liquid after the ultrafiltration advances people's spray-drier and carries out drying, obtains the N,O-Diacetylmuramidase of white powder.
Yolk is added the NaCl solution dilution of 0.1M according to 1: 2 volume ratio, stir, the pH that regulates diluent with HCl is 5.2, and the limit adds the HCl limit stirs, 4 ℃ of standing over night, and 8000rpm is centrifugal, obtains yolk clear liquid and egg yolk solid thing.
In the yolk clear liquid, add ammonium sulfate, be made into 40% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 2.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 32% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 5.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 30% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 8.Solution enters the ultrafilter ultrafiltration, carries out desalination and concentrated.Liquid after the ultrafiltration advances people's spray-drier and carries out drying, obtains the slightly yolk antibody of yellow powder shape of white.
The egg yolk solid thing is added 35 ℃ dehydrated alcohol according to 1: 12 mass ratio, and it is ultrasonic while stirring to put into ultrasonic apparatus, and ultrasonic frequency is 80Hz, and the time is 0.5h.Non-shock chilling to 20 ℃, 300～500 order filtered through gauze, the solids that obtains is for containing a small amount of proteinic Oil of egg yolk.Filtrate is carried out underpressure distillation, is concentrated to 1/6 of original volume, adds 2 times acetone, stir, and 300～500 order filtered through gauze, solids is dried in 45 ℃ of baking ovens, obtains Yelkin TTS.Filtrate is carried out underpressure distillation and is concentrated, and dries in 45 ℃ of baking ovens, obtains yolk cholesterol.
Example 4
Egg is cleaned, opened, take out egg white and yolk, obtain Ovum crusta Gallus domesticus.Ovum crusta Gallus domesticus 60 ℃ of oven dry down, is crushed to 300 orders with pulverizer then, obtains being used for the egg-shell meal of animal-feed.
Utilize the egg separator that egg white is separated with yolk, obtain egg white and yolk.
With the volume ratio thin up of egg white according to 1: 2, stir, stirring velocity is advisable with non-foaming.Above-mentioned egg white diluent is transferred pH to 7 with HCl, wait to do the sample liquid of ion-exchange.Acrylic type macropore weak cation exchange resin is carried out pre-treatment with 0.1M HCl, 0.1M NaOH respectively, be exchanged into the Na type at last.Mix at 3: 1 by sample liquid and acrylic type macropore weak cation exchange resin mass ratio, stir 2h under the room temperature, filter.Collect filtrate, spray-drier carries out drying, obtains removing the egg-white powder of the white powder of N,O-Diacetylmuramidase.Resin with the washed with de-ionized water of pH 73 times, is removed impurity and foreign protein.Press the isopyknic amount of resin, add 10% ammoniumsulphate soln, slowly stir 0.5h under the room temperature, filter resin, get elutriant.Clean resin 3 times, and 3 elutriants are merged.Elutriant after merging is entered the ultrafilter ultrafiltration, carry out desalination and concentrated.Liquid after the ultrafiltration advances people's spray-drier and carries out drying, obtains the N,O-Diacetylmuramidase of white powder.
Yolk is added the NaCl solution dilution of 0.1M according to 1: 4 volume ratio, stir, the pH that regulates diluent with HCl is 5.2, and the limit adds the HCl limit stirs, 4 ℃ of standing over night, and 8000rpm is centrifugal, obtains yolk clear liquid and egg yolk solid thing.
In the yolk clear liquid, add ammonium sulfate, be made into 40% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 3.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 32% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 6.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 30% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains.Precipitation added in the entry dissolve, the ratio of the consumption of water and former yolk clear liquid is 1: 10.Solution enters the ultrafilter ultrafiltration, carries out desalination and concentrated.Liquid after the ultrafiltration advances people's spray-drier and carries out drying, obtains the slightly yolk antibody of yellow powder shape of white.
The egg yolk solid thing is added 45 ℃ dehydrated alcohol according to 1: 16 mass ratio, and it is ultrasonic while stirring to put into ultrasonic apparatus, and ultrasonic frequency is 150Hz, and the time is 1h.Non-shock chilling to 25 ℃, 300～500 order filtered through gauze, the solids that obtains is for containing a small amount of proteinic Oil of egg yolk.Filtrate is carried out underpressure distillation, is concentrated to 1/8 of original volume, adds 3 times acetone, stir, and 300～500 order filtered through gauze, solids is dried in 45 ℃ of baking ovens, obtains Yelkin TTS.Filtrate is carried out underpressure distillation and is concentrated, and dries in 45 ℃ of baking ovens, obtains yolk cholesterol.
Claims (11)
1. high added value egg active composition comprehensive exploitation method, product comprises N,O-Diacetylmuramidase, Yelkin TTS, yolk antibody, cholesterol, Oil of egg yolk, egg-white powder and egg-shell meal.It is characterized in that may further comprise the steps:
(1) separation of egg main ingredient:
A cleans egg, opens, and takes out egg white and yolk, obtains Ovum crusta Gallus domesticus;
B utilizes the egg separator that egg white is separated with yolk, obtains egg white and yolk;
C, the NaCl water that yolk is added 0.1M dilutes, and stirs, and the pH that regulates diluent with HCl is 5.2, and the limit adds the HCl limit stirs, 4 ℃ of standing over night, 8000rpm is centrifugal, obtains yolk clear liquid and egg yolk solid thing;
(2) Ovum crusta Gallus domesticus is dried down at 60 ℃, be crushed to 300 orders with pulverizer then, obtain being used for the egg-shell meal of animal-feed;
(3) extraction of N,O-Diacetylmuramidase and egg-white powder and preparation:
A with the egg white thin up, stirs, and stirring velocity is advisable with non-foaming;
B with sample pH on the HCl accent, waits to do the sample liquid of ion-exchange with above-mentioned egg white diluent;
C carries out pre-treatment with 0.1M HCl, 0.1M NaOH respectively with acrylic type macropore weak cation exchange resin, is exchanged into the Na type at last;
D mixes by sample liquid and acrylic type macropore weak cation exchange resin, stirs 2h under the room temperature, filters.Collect filtrate, spray-drier carries out drying, obtains removing the egg-white powder of the white powder of N,O-Diacetylmuramidase;
E with the washed with de-ionized water 3 times that resin is used sample pH, removes impurity and foreign protein;
F presses the isopyknic amount of resin, adds 10% ammoniumsulphate soln, slowly stirs 0.5h under the room temperature, filters resin, gets elutriant.Clean resin 3 times, and 3 elutriants are merged;
G enters the ultrafilter ultrafiltration with the elutriant after merging, and carries out desalination and concentrated;
H, the liquid after the ultrafiltration advance people's spray-drier and carry out drying, obtain the N,O-Diacetylmuramidase of white powder;
(4) extraction of yolk antibody:
A with the yolk clear liquid that obtains among the step 1) c, adds ammonium sulfate, is made into 40% saturation concentration, and transfers pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains;
B adds precipitation in the entry and to dissolve.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 32% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains;
C adds precipitation in the entry and to dissolve.Filter and remove insolubles, add ammonium sulfate in the filtrate, be configured to 30% saturation concentration, and transfer pH to 5.2,4 ℃ of standing over night, the centrifugal precipitation that obtains;
D adds precipitation in the entry and to dissolve.Solution enters the ultrafilter ultrafiltration, carries out desalination and concentrated;
E, the liquid after the ultrafiltration advance people's spray-drier and carry out drying, obtain the slightly yolk antibody of yellow powder shape of white;
(5) extraction of Yelkin TTS, cholesterol and Oil of egg yolk:
A adds dehydrated alcohol with the egg yolk solid thing that obtains among the step 1) c, and it is ultrasonic while stirring to put into ultrasonic apparatus;
B, non-shock chilling, 300～500 order filtered through gauze, the solids that obtains is for containing a small amount of proteinic Oil of egg yolk;
C, filtrate is carried out underpressure distillation and is concentrated, and adds acetone, stir, 300～500 order filtered through gauze, solids is dried in 45 ℃ of baking ovens, obtains Yelkin TTS;
D, above-mentioned filtrate is carried out underpressure distillation and is concentrated, and dries in 45 ℃ of baking ovens, obtains yolk cholesterol.
2. high added value egg active composition comprehensive exploitation method according to claim 1, the volume ratio that it is characterized in that the NaCl of step (1) c mesolecithal and 0.1M is 1: 2～1: 4.
3. height according to claim 1 adds egg active composition comprehensive exploitation method, it is characterized in that the volume ratio of egg white and water is 1: 0.5～1: 2 among step (3) a.
4. height according to claim 1 adds egg active composition comprehensive exploitation method, it is characterized in that going up sample pH among step (3) b is 6～7.
5. height according to claim 1 adds egg active composition comprehensive exploitation method, it is characterized in that among step (3) c sample liquid and acrylic type macropore weak cation exchange resin mass ratio 5: 1～3: 1.
6. height according to claim 1 adds egg active composition comprehensive exploitation method, it is characterized in that the consumption of water among step (4) b and the ratio of former yolk clear liquid are 1: 2～1: 3.
7. height according to claim 1 adds egg active composition comprehensive exploitation method, it is characterized in that the consumption of step (4) c water and the ratio of former yolk clear liquid are 1: 5～1: 6.
8. height according to claim 1 adds egg active composition comprehensive exploitation method, it is characterized in that the consumption of step (4) d water and the ratio of former yolk clear liquid are 1: 8～1: 10.
9. height according to claim 1 adds egg active composition comprehensive exploitation method, the mass ratio that it is characterized in that step (5) a mesolecithal solids and dehydrated alcohol is 1: 12～1: 16, the temperature of dehydrated alcohol is 35～45 ℃, ultrasonic frequency is 80～150Hz, and ultrasonic time is 0.5～1h.
10. height according to claim 1 adds egg active composition comprehensive exploitation method, it is characterized in that the refrigerative temperature is 20～25 ℃ among step (5) b.
11. height according to claim 1 adds egg active composition comprehensive exploitation method, it is characterized in that spissated volume is to 1/6～1/8 of original volume among step (5) c, the consumption of acetone is 2～3 times of concentrated rear filtrate volume.
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| CN102863469A (en) * | 2012-09-10 | 2013-01-09 | 江苏大学 | Preparation method of high-purity egg yolk phosphatidylcholine (PC) |
| CN103238643A (en) * | 2012-09-24 | 2013-08-14 | 河北同和生物制品有限公司 | Biological calcium supplement special for flour |
| CN103355498A (en) * | 2013-07-26 | 2013-10-23 | 河南碧云天饲料有限公司 | Biological feed composite, compound premix and milk replacer without antibiotic or plasma protein for suckling pigs |
| CN104327184A (en) * | 2014-09-28 | 2015-02-04 | 浙江省长兴艾格生物制品有限公司 | Preparation method of yolk globulin powder |
| CN105754708A (en) * | 2016-05-20 | 2016-07-13 | 河南省奥林特药业有限公司 | Industrial production method for egg yolk oil |
| CN107083269A (en) * | 2017-04-01 | 2017-08-22 | 新疆喀什昆仑翠翎鸽业有限责任公司 | One kind is without BaP pigeon eggs butter and preparation method thereof |
| CN107383187A (en) * | 2017-07-10 | 2017-11-24 | 华中农业大学 | A kind of combined extracting IgY from egg yolk, phosvitin, lecithin, the method for egg oil and de-fatted egg yolk powder |
| CN108084243A (en) * | 2017-12-28 | 2018-05-29 | 广州白云山汉方现代药业有限公司 | A kind of method that high-purity cholesterol is extracted from egg oil |
| CN108396018A (en) * | 2018-01-18 | 2018-08-14 | 青海华杰实业有限公司 | The preparation method of plateau special cultivation rare bird egg egg white lysozyme |
| CN114376934A (en) * | 2022-02-23 | 2022-04-22 | 广州葆妍生物科技有限公司 | Natural mild repair emulsifier composition and preparation method and application thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102863469A (en) * | 2012-09-10 | 2013-01-09 | 江苏大学 | Preparation method of high-purity egg yolk phosphatidylcholine (PC) |
| CN102863469B (en) * | 2012-09-10 | 2015-04-22 | 江苏大学 | Preparation method of high-purity egg yolk phosphatidylcholine (PC) |
| CN103238643B (en) * | 2012-09-24 | 2014-09-24 | 河北同和生物制品有限公司 | Biological calcium supplement special for flour |
| CN103238643A (en) * | 2012-09-24 | 2013-08-14 | 河北同和生物制品有限公司 | Biological calcium supplement special for flour |
| CN103355498A (en) * | 2013-07-26 | 2013-10-23 | 河南碧云天饲料有限公司 | Biological feed composite, compound premix and milk replacer without antibiotic or plasma protein for suckling pigs |
| CN103355498B (en) * | 2013-07-26 | 2014-10-22 | 河南碧云天饲料有限公司 | Biological feed composite, compound premix and milk replacer without antibiotic or plasma protein for suckling pigs |
| CN104327184A (en) * | 2014-09-28 | 2015-02-04 | 浙江省长兴艾格生物制品有限公司 | Preparation method of yolk globulin powder |
| CN105754708A (en) * | 2016-05-20 | 2016-07-13 | 河南省奥林特药业有限公司 | Industrial production method for egg yolk oil |
| CN107083269A (en) * | 2017-04-01 | 2017-08-22 | 新疆喀什昆仑翠翎鸽业有限责任公司 | One kind is without BaP pigeon eggs butter and preparation method thereof |
| CN107383187A (en) * | 2017-07-10 | 2017-11-24 | 华中农业大学 | A kind of combined extracting IgY from egg yolk, phosvitin, lecithin, the method for egg oil and de-fatted egg yolk powder |
| CN108084243A (en) * | 2017-12-28 | 2018-05-29 | 广州白云山汉方现代药业有限公司 | A kind of method that high-purity cholesterol is extracted from egg oil |
| CN108396018A (en) * | 2018-01-18 | 2018-08-14 | 青海华杰实业有限公司 | The preparation method of plateau special cultivation rare bird egg egg white lysozyme |
| CN114376934A (en) * | 2022-02-23 | 2022-04-22 | 广州葆妍生物科技有限公司 | Natural mild repair emulsifier composition and preparation method and application thereof |
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