CN102226150A - Cell cultivating apparatus used for imaging living cell - Google Patents
Cell cultivating apparatus used for imaging living cell Download PDFInfo
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- CN102226150A CN102226150A CN2011101233240A CN201110123324A CN102226150A CN 102226150 A CN102226150 A CN 102226150A CN 2011101233240 A CN2011101233240 A CN 2011101233240A CN 201110123324 A CN201110123324 A CN 201110123324A CN 102226150 A CN102226150 A CN 102226150A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/44—Multiple separable units; Modules
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a cell cultivating apparatus used for imaging a living cell, which comprises a base plate, a substrate and a cover plate, at least 8 cavities which are penetrated from top to bottom are arranged on the upper surface of the substrate, a plurality of stiffeners are arranged on the upper surface of the substrate and around the surrounding regions of the cavities, the substrate is fixed on the base plate with thickness of 0.17mm; the cover plate is used for covering above the cavities, a handle is arranged on one side of the cover plate; the number of the cavities is 8; a groove is provided on the lower surface of the substrate and around the surrounding regions of the cavities; the substrate is fixedly connected with the base plate through an adhesive layer positioned on the groove; the cavities are divided into two arrays and arranged in parallel on the upper surface of the substrate; the cavities are square cavities. The cell cultivating apparatus is used for researches of imaging a living cell with high flux and high resolution; and is convenient for operators to operate, a parallel research of cells under a plurality of test conditions at a time can be realized.
Description
Technical field
The present invention relates to cell culture apparatus, be specifically related to a kind of cell culture apparatus that is used for the viable cell imaging.
Background technology
In view of the widespread use of microscopy in Basic of Biology research and medicine industry, various viable cell microscope imaging technology become the important tool in the biomedical research.Go for high-resolution viable cell micro-image, often need to use the microlens (40X, 63X, 100X etc.) of high power, this moment for the thickness of Tissue Culture Dish base material and specific refraction etc. require just to become very harsh.But the correlation parameters such as thickness of at present common cell cultures consumptive material base material all do not satisfy the demand of high-resolution imaging.Make and carry out the very complexity that high resolution viable cell imaging process becomes, often cell need be grown in earlier on the cover glass, then cover glass is fixed in the complicated apparatus system, allow microlens directly carry out imaging in the face of cover glass.Its process is not only very complicated, consuming time long again, and often being accompanied by nutrient solution leakage, slide glass, may to be exposed to time in the air oversize and influence the function of cell, can not carry out shortcomings such as high-throughput, high-resolution imaging.
Summary of the invention
The invention provides a kind of cell culture apparatus that is used for the viable cell imaging, this cell culture apparatus is adapted to the research of high-throughput, the imaging of high resolution viable cell; And device has made things convenient for experimenter's operation, has realized disposablely can carrying out parallel study to the cell under a plurality of different test conditionss.
For achieving the above object, the technical solution used in the present invention is: a kind of cell culture apparatus that is used for the viable cell imaging comprises: substrate and cover plate, and this upper surface of base plate is provided with the cavity of at least 8 up/down perforations; Described base lower surface and be provided with groove around described cavity peripheral regions, described substrate is fixedlyed connected with described base plate by the binder layer that is positioned at described groove; Described upper surface of base plate and be provided with plurality of strengthening ribs around described cavity peripheral regions; Described substrate is fixed on the described base plate, and this base plate thickness is 0.17mm; Described cover plate is used to cover described cavity top, and this cover plate one side has the leader.
Related content in the technique scheme is explained as follows:
1, in the such scheme, described cavity numbers is 8.
2, in the such scheme, described cavity is divided into two rows and is set in parallel in described upper surface of base plate.
3, in the such scheme, described cavity is square cavity, and this cavity cross-section is long and wide size is 0.8cm*0.8cm.
Because the technique scheme utilization, the present invention compared with prior art has following advantage and effect:
1, cell culture apparatus of the present invention is provided with plurality of strengthening ribs and has reinforced cavity, and handle has made things convenient for experimenter's operation.
2, cell culture apparatus of the present invention has been realized high-throughput; On a substrate, have 8 cavitys, binder layer and the described base plate adhering junction of substrate by being positioned at described groove, bonding effect is good, prevents the crossed contamination between each cavity, therefore can carry out a plurality of parallel tests simultaneously.Improve flux and speed, improved the efficient of viable cell imaging greatly.
3, cell culture apparatus cavity cross-section of the present invention is of a size of 0.8cm*0.8cm, therefore the cultivation liquid that is consumed, need the cell etc. of inoculation also just corresponding reduce a lot, need the 200ul nutrient solution such as each cavity of the present invention, the conventional then minimum cultivation liquid that needs 2ml of consumptive material has been saved consumptive material consumption; And cultured cells can directly be carried out the relevant research of microscope.
Description of drawings
Accompanying drawing 1 is cell culture apparatus structural representation of the present invention;
Accompanying drawing 2 is board structure synoptic diagram of the present invention.
In the above accompanying drawing: 1, base plate; 2, substrate; 3, cover plate; 4, cavity; 5, handle; 6, stiffening web; 7, groove.
Embodiment
Below in conjunction with drawings and Examples the present invention is further described:
Embodiment: a kind of cell culture apparatus that is used for the viable cell imaging, shown in accompanying drawing 1-2, comprising: base plate 1 also comprises substrate 2 and cover plate 3, these substrate 2 upper surfaces are provided with the cavity 4 of at least 8 up/down perforations, substrate 2 lower surfaces and be provided with groove 7 around described cavity 4 peripheral regions; Described substrate 2 is fixedlyed connected with described base plate 1 by the binder layer that is positioned at described groove 5; Described substrate 2 upper surfaces and be provided with plurality of strengthening ribs 6 around described cavity 4 peripheral regions, described substrate 2 is fixed on the described base plate 1, and this base plate 1 thickness is 0.17mm, and material is a glass; Described cover plate 3 is used to cover described cavity 4 tops, and these cover plate 3 one sides have leader 5.
Above-mentioned cavity 4 numbers are 8.
Above-mentioned cavity 4 is divided into two rows and is set in parallel in described substrate 2 upper surfaces.
Above-mentioned cavity 4 is square cavity, and these cavity 4 cross sections are long and wide size is 0.8cm*0.8cm.
When adopting above-mentioned cell culture apparatus, be provided with plurality of strengthening ribs and reinforced cavity, and handle has made things convenient for experimenter's operation; Secondly, realize high-throughput, on a substrate, had 8 cavitys, binder layer and the described base plate adhering junction of substrate by being positioned at described groove, bonding effect is good, prevents the crossed contamination between each cavity, therefore can carry out a plurality of parallel tests simultaneously.Improve flux and speed, improved the efficient of viable cell imaging greatly; Once more, cavity cross-section is of a size of 0.8cm*0.8cm, the cultivation liquid that is therefore consumed, needs that the cell etc. of inoculation is also just corresponding to be reduced a lot, needs the 200ul nutrient solution such as each cavity of the present invention, the conventional then minimum cultivation liquid that needs 2ml of consumptive material has been saved consumptive material consumption; And cultured cells is enough carried out the relevant research of microscope.
The foregoing description only is explanation technical conceive of the present invention and characteristics, and its purpose is to allow the personage who is familiar with this technology can understand content of the present invention and enforcement according to this, can not limit protection scope of the present invention with this.All equivalences that spirit is done according to the present invention change or modify, and all should be encompassed within protection scope of the present invention.
Claims (4)
1. a cell culture apparatus that is used for the viable cell imaging comprises base plate (1), it is characterized in that: also comprise substrate (2) and cover plate (3), this substrate (2) upper surface is provided with the cavity (4) of at least 8 up/down perforations; Described substrate (2) lower surface and be provided with groove (7) around described cavity (4) peripheral regions, described substrate (2) is fixedlyed connected with described base plate (1) by the binder layer that is positioned at described groove (5); Described substrate (2) upper surface and be provided with plurality of strengthening ribs (6) around described cavity (4) peripheral regions; Described substrate (2) is fixed on the described base plate (1), and this base plate (1) thickness is 0.17mm; Described cover plate (3) is used to cover described cavity (4) top, and these cover plate (3) one sides have leader (5).
2. cell culture apparatus according to claim 1 is characterized in that: described cavity (4) number is 8.
3. cell culture apparatus according to claim 2 is characterized in that: described cavity (4) is divided into two rows and is set in parallel in described substrate (2) upper surface.
4. according to each described cell culture apparatus of claim 1-3, it is characterized in that: described cavity (4) is square cavity, and this cavity (4) cross section is long and wide size is 0.8cm*0.8cm.
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CN2011101233240A CN102226150A (en) | 2011-05-13 | 2011-05-13 | Cell cultivating apparatus used for imaging living cell |
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CN2011101233240A CN102226150A (en) | 2011-05-13 | 2011-05-13 | Cell cultivating apparatus used for imaging living cell |
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Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1204363A (en) * | 1995-10-06 | 1999-01-06 | 麦克罗克隆宁Cccd公司 | Compact cell culture slide |
WO2001032917A1 (en) * | 1997-11-21 | 2001-05-10 | Csm, Inc. | Method of cell sedimentation using precision deposit manifold |
WO2006005811A1 (en) * | 2004-07-09 | 2006-01-19 | Chip-Man Technologies Oy | A substructure for cultivating cells and its use |
CN2863808Y (en) * | 2005-12-19 | 2007-01-31 | 祖述冲 | Cell multiple-grooved incubator |
CN1928063A (en) * | 2006-08-31 | 2007-03-14 | 李宏 | High-density array type cell slide culture apparatus, storage apparatus and experimental apparatus |
CN201395600Y (en) * | 2009-02-27 | 2010-02-03 | 中国人民解放军第三军医大学第一附属医院 | Integrated cell culture device with separable substrate |
CN101876748A (en) * | 2009-04-28 | 2010-11-03 | 中国科学院理化技术研究所 | Reusable special cell culture dish of laser confocal microscope |
CN201695031U (en) * | 2010-05-17 | 2011-01-05 | 复旦大学 | Partitioned culture vessel with glass bottom |
CN202089993U (en) * | 2011-05-13 | 2011-12-28 | 刘小龙 | Cell culture device for imaging of living cells |
-
2011
- 2011-05-13 CN CN2011101233240A patent/CN102226150A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1204363A (en) * | 1995-10-06 | 1999-01-06 | 麦克罗克隆宁Cccd公司 | Compact cell culture slide |
WO2001032917A1 (en) * | 1997-11-21 | 2001-05-10 | Csm, Inc. | Method of cell sedimentation using precision deposit manifold |
WO2006005811A1 (en) * | 2004-07-09 | 2006-01-19 | Chip-Man Technologies Oy | A substructure for cultivating cells and its use |
CN2863808Y (en) * | 2005-12-19 | 2007-01-31 | 祖述冲 | Cell multiple-grooved incubator |
CN1928063A (en) * | 2006-08-31 | 2007-03-14 | 李宏 | High-density array type cell slide culture apparatus, storage apparatus and experimental apparatus |
CN201395600Y (en) * | 2009-02-27 | 2010-02-03 | 中国人民解放军第三军医大学第一附属医院 | Integrated cell culture device with separable substrate |
CN101876748A (en) * | 2009-04-28 | 2010-11-03 | 中国科学院理化技术研究所 | Reusable special cell culture dish of laser confocal microscope |
CN201695031U (en) * | 2010-05-17 | 2011-01-05 | 复旦大学 | Partitioned culture vessel with glass bottom |
CN202089993U (en) * | 2011-05-13 | 2011-12-28 | 刘小龙 | Cell culture device for imaging of living cells |
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Application publication date: 20111026 |