CN102204508A - Interspecific cross breeding method for cucurbit (melon rootstock) - Google Patents

Interspecific cross breeding method for cucurbit (melon rootstock) Download PDF

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Publication number
CN102204508A
CN102204508A CN2010101549959A CN201010154995A CN102204508A CN 102204508 A CN102204508 A CN 102204508A CN 2010101549959 A CN2010101549959 A CN 2010101549959A CN 201010154995 A CN201010154995 A CN 201010154995A CN 102204508 A CN102204508 A CN 102204508A
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China
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melon
rootstock
watermelon
cucurbit
grafting
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CN2010101549959A
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王毓洪
应泉盛
王迎儿
孟秋峰
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Ningbo Academy of Agricultural Sciences
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Ningbo Academy of Agricultural Sciences
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Priority to CN2010101549959A priority Critical patent/CN102204508A/en
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Abstract

The invention belongs to the technical field of breeding of cucurbitaceae vegetable. An interspecific hybrid F1 (Yong rootstock No. 6) of cucurbit (a melon rootstock) is obtained by hybridizing a female parent of a stable inbred line J03-2-3-2-1-5-7-4 by multi-generation of directed autocopuation screening of cucurbit materials originated from Japan and a male parent of a stable inbred line YH2-3-3-2-1-4-2-6 by multi-generation of directed autocopuation screening of local Lagenaria species produced in Ningbo. The melon rootstock selectively bred with the method provided in the invention meets objectives of breeding and has moderate growth potential, high resistance to die-back and root rot, well-developed roots, strong stalks, strong ability in absorbing nutrient, low degree of vain growth and robust hypocotyls which are not easy to become hollow, and therefore the melon rootstock is favorable for grafting. The melon rootstock has good grafting affinity and strong symbiosis affinity, and a survival rate over 95% is obtained for the grafting of the rootstock onto the watermelon specie ''Zaojia'' (8424). The engrafted melon rootstock is low temperature-resistant, moisture-resistant and barren-resistant; after field planting, the root and overground part of a grafted seedling grows fast and has sound growth potential, and the period of first harvest of the grafted seedling is 5 days earlier than that of a self-rooted seedling, the fruition rate is high and stable, weight of single fruit and fruit shape can substantially maintain the same, melon skin is not thickened, sugar degree of the grafted seedling is higher than that of the self-rooted seedling, sugar degree gradient is small, quality of the watermelon is not affected, and good adaptability is obtained.

Description

The interspecific cross breeding method of a kind of cucurbit (melon stock)
Technical field
The invention belongs to the cucurbitaceae vegetable breeding technical field, relate to the selection of cucurbit (melon stock) sexual hybridization kind, the seed selection that ' No. 6, river in Zhejiang Province anvil ' newly made up in cucurbit (melon stock) sexual hybridization specifically.
Background technology
Watermelon is long at China's cultivation history, and it is wide to distribute, and supply time is long, is the main fruit in spring, summer, autumn, liked by the consumer, and wherein Zhejiang Province's area under cultivation in 2008 is 1,430,000 mu, is one of economic crops of Zhejiang Province's sowing area maximum.Therefore, the sound development direct relation of watermelon industry increasing peasant income, growth of agricultural efficiency.In recent years rice, because the rapid expansion of melon crop facility cultivation area and improving constantly of cropping index, cause the rotation cycle to shorten, soil continuous cropping obstacle has the gesture that increases the weight of, especially soil-borne disease such as cucurbits fusarium wilt owing to reasons such as continuous croppings, has the trend that increases the weight of year by year, withered strain rate reaches 20~30%, and weight person destroys in full field.The pathogen of these diseases mainly is to survive the winter in soil, invalid body reach without the muck that becomes thoroughly decomposed with mycelium, chlamydospore, sclerotium, is soil inhabitant, and indomitable vitality is arranged in soil, and invalid body decomposes the back germ can survive in soil 5~6 years.Take the way diseases prevention of crop rotation, need the cycle long, the crop species of protection ground cultivation is limited in addition, is difficult to carry out, and therefore, soil-borne disease has become a big obstacle that influences melon crop stable high yield.At present, control watermelon soil-borne disease valid approach the most is a grafting and root-changing, and the special-purpose stock of suitable watermelon grafting is the key factor that guarantees quality of watermelon, improves output.But the seed selection work of current domestic melon graft stock is ground zero, and kind lacks; And the seed quality of external import is good and on the high side, has influenced the promotion rate of watermelon graft technology in China.Because Zhejiang is rainy humid region, some domestic variety lower temperature resistances, wet resistance are poor, and root rot takes place seriously under the spring low temperature and high relative humidity condition, is not suitable for cultivating in this locality.With resistance, influence the cucurbit material of quality of watermelon by force and not with watermelon grafting affinity, symbiosis affinity, and with low temperature resistant, moisture-proof, low light tolerance and local adaptable ' Ningbo nightflower ' bottle gourd material as the parent, the F1 seed that interspecific cross obtains has resistance,, symbiosis affinity strong with the watermelon grafting affinity concurrently by force, does not influence characteristics such as quality of watermelon, low temperature resistant, moisture-proof, in the document report is not arranged at home and abroad as the graft stock kind of local watermelon.
Summary of the invention
Purpose of the present invention
The present invention solves to produce at present to go up that domestic melon stock variety is poor in Zhejiang area bad adaptability, wet resistance, root rot takes place serious, problems such as external good stock variety seed is on the high side, a kind of selection of melon stock sexual hybridization kind is provided, select anti-blight, root rot, plant the variety of watermelon grafting affinity and symbiosis affinity influences quality of watermelon, high yield by force and not with main, the melon stock new varieties of suitable Zhejiang area cultivation condition solve the problem that lacks graft stock kind of high quality and at a reasonable price in the watermelon production.
The selection of cucurbit provided by the present invention (melon stock) crossbreed:
The stable inbred line J03-2-3-2-1-5-7-4 that filter out with the many directed selfings of generation of cucurbit material warp that derive from Japan are female parent, is male parent with Ningbo local bottle gourd kind nightflower through the stable inbred line YH2-3-3-2-1-4-2-6 that many directed selfings of generation filter out, and hybridizes to obtain cucurbit (melon stock) interspecific cross kind F1 (' No. 6, river in Zhejiang Province anvil ').
Main feature of the present invention is:
Cucurbit has well developed root system, high resistance to wilt good, by force, does not influence characteristics such as quality of watermelon with watermelon affinity and symbiosis affinity as melon graft stock, and Ningbo local bottle gourd kind nightflower has precocity, low temperature resistant, characteristics such as wet resistance is strong, anti-root rot.Is that maternal, nightflower is a male parent with the cucurbit, filial generation has cucurbit concurrently to be had well developed root system, high resistance to wilt good, by force, not to influence quality of watermelon with watermelon affinity and symbiosis affinity, and nightflower low temperature resistant, wet resistance is strong, the characteristics of anti-root rot.Because Zhejiang area is rainy moistening, require melon graft stock except that general stock requirement characteristics, also require strong, the anti-root rot of wet resistance, early spring tolerance to low temperature and weak light etc., first familiar generation with cucurbit and Ningbo bottle gourd landrace has these characteristics, suitable stock variety as local watermelon grafting.
Advantage of the present invention and good effect are as follows:
This kind well developed root system, cane is sturdy, and absorption ability of nutrient is strong, and branches and leaves are difficult for excessive growth, and hypocotyl is sturdy to be difficult for hollowly, helps the grafting operation; Grafting affinity is good, and the symbiosis compatibility is strong, the graft survival rate height; Anti-blight, root rot, growth potential is sane, and fruiting rate is high and stablize, and is low temperature resistant, and moisture-proof is barren-resistant, does not influence quality of watermelon, and the fruit increase is not obvious, and effect of increasing production is obvious; Be suitable for type watermelons such as medium-sized, the watermelon grafting cultivation of different planting such as booth, open country, adaptability is good.2007~2008 years variety comparative test output is 3526~4678kg/667m 2, average yield per mu 4038kg, it is suitable with the magic power output of Japanese stock variety to plant the glad anvil in watermelon-stock capital No. 1 with the master, be higher than stock varieties such as super rich F1, warrier, high anti-watermelon blight after the grafting, high anti-root rot, and the more above-mentioned kind of resistance is stronger, grafting watermelon single fruit weight and fruit shape remain unchanged substantially, increase to some extent but sit the melon number for same batch, pol is than the own-rooted tree height, and quality is unaffected, receipts phase beginning was than 8424 own-rooted trees early 5~7 days, than other stock varieties 2~3 days ahead of time.This stock variety cucurbit seedling growth potential is strong, main climing thick about 1.4cm, and long and wide 24cm and the 36cm of being about respectively of maximum leaf, the lateral bine result, first female flower joint position is the climing first segments of climing 8~10 joint position grandsons of son.Fruit is shaped as pyriform, the green white of color of the leather, and individual plant can stay 6~8 of fruits, blooms to kind of the fruit of gathering per mu yield seed 50~75kg about 60 days.The seed rectangle, white brown, the kind shell is hard, the about 127g of thousand kernel weight.
The present invention is achieved by the following technical programs:
(1) cultivar origin: the stable inbred line J03-2-3-2-1-5-7-4 that filter out with the many directed selfings of generation of cucurbit material warp that derive from Japan are female parent, and the local bottle gourd kind nightflower stable inbred line YH2-3-3-2-1-4-2-6 that directed selfings of generation filter out through how are male parent with Ningbo.
(2) parent's characteristic:
(1) Japanese cucurbit: the fruit pyriform, pericarp is green light green alternate, precocity, growing way is vigorous, and well developed root system, high resistance to wilt good,, grafting strong with watermelon affinity and symbiosis affinity do not influence characteristics such as quality of watermelon.
(2) Ningbo nightflower: show as ripe property early, growth potential is strong, and is more low temperature resistant, strong stress resistance, moisture-proof, and the long clavate of fruit, melon is about 50 centimetres, about 4.9 centimetres of melon transverse diameter, melon skin green.
(3) ' No. 6, river in Zhejiang Province anvil ' feature:
This kind well developed root system, cane is sturdy, and absorption ability of nutrient is strong, and branches and leaves are difficult for excessive growth, and hypocotyl is sturdy to be difficult for hollowly, helps the grafting operation; Grafting affinity is good, and the symbiosis compatibility is strong, the graft survival rate height; Anti-blight, root rot, growth potential is sane, and fruiting rate is high and stablize, and is low temperature resistant, and moisture-proof is barren-resistant, does not influence quality of watermelon, and the fruit increase is not obvious, and effect of increasing production is obvious; Be suitable for type watermelons such as medium-sized, the watermelon grafting cultivation of different planting such as booth, open country, adaptability is good.2007~2008 years variety comparative test output is 3526~4678kg/667m 2Average yield per mu 4038kg, the main watermelon-stock ' No. 1, the glad anvil in capital ' of planting is suitable with Japanese stock variety ' magic power ' output, be higher than ' stock varieties such as super rich F1 ', ' warrier ', high anti-watermelon blight after the grafting, high anti-root rot, and more above-mentioned varietal resistance is stronger, grafting watermelon single fruit weight and fruit shape remain unchanged substantially, increase to some extent but sit the melon number for same batch, pol is than the own-rooted tree height, and quality is unaffected, receipts phase beginning is ' early good ' (8424) own-rooted tree early 5~7 days, than other stock varieties 2~3 days ahead of time.This stock variety cucurbit seedling growth potential is strong, main climing thick about 1.4cm, and long and wide 24cm and the 36cm of being about respectively of maximum leaf, the lateral bine result, first female flower joint position is the climing first segments of climing 8~10 joint position grandsons of son.Fruit is shaped as pyriform, the green white of color of the leather, and individual plant can stay 6~8 of fruits, blooms to kind of the fruit of gathering per mu yield seed 50~75kg about 60 days.The seed rectangle, white brown, the kind shell is hard, the about 127g of thousand kernel weight.
Description of drawings
Embodiment
Further specify the present invention below in conjunction with instantiation.
Carry out the example that is applied as of breeding of new variety in Zhejiang with 2003-2007, step is as follows:
By extensive collection cucurbit, bottle gourd germplasm materials, obtained two parts of materials of the local bottle gourd kind (YH2) in Japanese cucurbit material (J03) and Ningbo in 2002, two parts of materials were through 2003~2006 years annual spring and autumn systematic breedings, obtain J03-2-2-1-5-7-4 and YH2-3-3-2-1-4-2-6 two part parent materials spring in 2006 behind the systematic breeding according to the breeding objective directive breeding, autumn in 2006 be female parent with J03-2-2-1-5-7-4, make up, and obtained F1 for seed (' No. 6, river in Zhejiang Province anvil ') with YH2-3-3-2-1-4-2-6 male parent preparing hybrid.
2007 and spring in 2008 ' No. 6, river in Zhejiang Province anvil ' carried out the grafting varietal yield test, planting variety of watermelon ' early good ' (8424) with the Zhejiang area master be scion, is contrast to lead watermelon graft stock ' No. 1, the glad anvil in capital ' and ' the magic power ' of planting.The grafting result of the test shows that the graft survival rate of ' No. 6, river in Zhejiang Province anvil ' is not remarkable with ' No. 1, the glad anvil in capital ' and ' magic power ' difference, and ' No. 6, river in Zhejiang Province anvil ' is good with ' early good ' (8424) grafting affinity, and ' No. 6, river in Zhejiang Province anvil ' hypocotyl slightly be difficult for hollow, easy grafting.After the field planting, ' No. 6, river in Zhejiang Province anvil ' because lower temperature resistance is strong, strong with watermelon symbiosis affinity, the early growth gesture is better than other anvil arts, the early flowering season was than other stocks early about 3 days.2007~2008 years variety comparative test output is 3526~4678kg/667m 2Average yield per mu 4038kg, the main watermelon-stock ' No. 1, the glad anvil in capital ' of planting is suitable with Japanese stock variety ' magic power ' output, the fusarium wilt plant is not found in the field, and grafting watermelon single fruit weight and fruit shape remain unchanged substantially, but same batch of seat melon number increases to some extent, pol is than the own-rooted tree height, quality was unaffected, and receipts phase beginning is than 8424 own-rooted trees early 5~7 days, than other stock varieties 2~3 days ahead of time.2008 because low temperature overcast and rainy day influence in spring, root rot appears in the grafting watermelon, through check, the root rot incidence of disease of ' No. 6, river in Zhejiang Province anvil ' and ' magic power ' is 2~3%, ' No. 1, the glad anvil in capital ' root rot incidence of disease is 28%, the high anti-root rot of ' No. 6, river in Zhejiang Province anvil ', and resistance is better than ' No. 1, the glad anvil in capital '.
Rose in 2009 this kind is carried out multiple spot test, demonstration popularization and expanding propagation.

Claims (1)

1. the selection of a new cucurbit (melon stock) sexual hybridization new varieties, it is characterized in that the cucurbit kind to derive from Japan is a female parent through too much stable inbred line (code name J03-2-3-2-1-5-7-4) for artificial orientation's seed selection, stable inbred line (code name YH2-3-3-2-1-4-2-6) with Ningbo local bottle gourd kind nightflower is a male parent, carry out the crossbreed ' No. 6, river in Zhejiang Province anvil ' that interspecific cross obtains, this kind stock that grafting is used as watermelon.
CN2010101549959A 2010-03-31 2010-03-31 Interspecific cross breeding method for cucurbit (melon rootstock) Pending CN102204508A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104272939A (en) * 2013-07-04 2015-01-14 中国种子集团有限公司 Cultivation method for summer squash seedlings planted at one time and harvested for multiple times throughout year
CN109329043A (en) * 2018-11-30 2019-02-15 宁波市农业科学研究院 A kind of selection of small seed type pumpkin rootstock kind

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104272939A (en) * 2013-07-04 2015-01-14 中国种子集团有限公司 Cultivation method for summer squash seedlings planted at one time and harvested for multiple times throughout year
CN104272939B (en) * 2013-07-04 2016-02-17 中国种子集团有限公司 A kind of custard squash kind melon one plants the breeding method received throughout the year
CN109329043A (en) * 2018-11-30 2019-02-15 宁波市农业科学研究院 A kind of selection of small seed type pumpkin rootstock kind
CN109329043B (en) * 2018-11-30 2021-07-02 宁波市农业科学研究院 Breeding method of small-seed type pumpkin rootstock variety

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Application publication date: 20111005