CN102199521A - Enclosed solid state fermentation bioreactor - Google Patents
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- CN102199521A CN102199521A CN2011100869846A CN201110086984A CN102199521A CN 102199521 A CN102199521 A CN 102199521A CN 2011100869846 A CN2011100869846 A CN 2011100869846A CN 201110086984 A CN201110086984 A CN 201110086984A CN 102199521 A CN102199521 A CN 102199521A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/36—Means for collection or storage of gas; Gas holders
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The invention discloses an enclosed solid state fermentation bioreactor. The bioreactor comprises a solid state fermenter, an air inlet system, a humidification system and a tail gas detection and collection system. The solid state fermenter is a sealed container, and a central shaft of the fermenter is provided with a reverse double spiral stirring paddle which allows materials to carry out bidirectional movement, so an adhesion problem which appears in stirring solid materials can be solved and a mixing effect can be enhanced. A top tail gas outlet of the fermenter is connected with an evacuation tube and a CO2 collector, and a fermentation process and a fermentation state are displayed through detecting the CO2 content. A jacket is arranged on the outside the fermenter, a bypass is arranged on a humidifier, and the fermentation bed temperature can be automatically adjusted through feeding back information to a control system by a thermocouple which is on an upper part of the fermenter to adjust solenoid valves of jacket cooling water and the humidifier bypass. The humidifier temperature and periods and times of stirring and spraying can be preset according to material fermentation characteristics. A bed overtemperature problem, which appears in a fermentation peak period and is most difficult to solve in solid fermentation processes, can be solved by removing heat of fermentation through a plurality of modes such as jacket cooling water adjusting, cold air accessing, atomized water spraying, material stirring and the like.
Description
Technical field
The present invention relates to a kind of systematize solid-state fermentation installation, particularly a kind of closed solid-state fermentation reaction device.
Background technology
In recent years and since solid state fermentation have water saving, energy-conservation, discharge few unique advantage, belong to clearer production technology, progressively obtain the attention of countries in the world.But compare with liquid state fermentation research, solid state fermentation lacks effectively research at aspects such as mass transfer, heat transfers, be difficult to realize industrial and large-scale production.Present making leaven of soy sauce etc. still mainly adopts bamboo plaque mode, and this mode mechanization degree is low, and labour intensity is big, and can not reach aseptic technique, particularly runs into the yellow plum sky, is easy to microbiological contamination.Therefore be badly in need of a kind of mechanize intensity height, the equipment of closed-loop operation.Occurred some brewing equipments now, but these equipment manufacturing cost costlinesses are difficult to large-scale promotion.A topmost problem is exactly a heat dissipation problem in solid state fermentation, because solid-state substrate heat conductance is poor, causes heat convection current and heat transfer efficiency low, on the heat production peak, bed temperature sharply rises, and thermograde occurs, must reduce the appearance of thermograde during the fermentation as far as possible.General by convection of air, the mode controlled temperature of evaporation, but must consider the effect of convection of air to moisture evaporation, prevent the moisture excessive loss.
Patent CN101496574 discloses a kind of closed solid-state fermentation reaction device, comprises inlet system, closed system, Controlling System.This jar by entering gas temperature and the temperature of flow control fermenting process, have whipping appts, can add moisture during the fermentation, can effectively control the solid state fermentation parameter, improve the quality and the output of solid state fermentation.This patent has further improved stirring, heat transfer type on the basis of patent CN101496574, and has added the tails assay function.
Summary of the invention
The objective of the invention is to provides a kind of closed solid-state fermentation reaction device on the basis of patent CN101496574, more effectively solve solid substances stirring, move heat, tails assay problem.
Closed solid-state fermentation reaction device provided by the invention comprises devices such as solid-state fermenter, inlet system, air humidifying system, Controlling System.
Described solid-state fermenter is a closed container, and the center is provided with a central shaft, and its upper end reaches outside the described closed container and is connected with motor by belt, on the described central shaft stirring rake is set, and starter motor drives the stirring rake rotation solid state reaction material is stirred.Described stirring rake is oppositely two ribbon stirring rakes, described reverse pair of ribbon stirring rake is made of two different ribbons of the opposite diameter of the hand of spiral, the ribbon width is the 1/10-1/30 of the diameter of described closed container, and the ribbon diameter is the 2/5-4/5 of the diameter of described closed container.
Fermentation tail gas outlet on the described solid state fermentation top ends of cans connects the CO2 collector, and this collector is connected with jar tail gas outlet and evacuated tube by T-valve.Described CO2 collector is one the container of sodium hydroxide solution to be housed easily, and concentration of sodium hydroxide solution is 0.1-1M, and dried fermentation tail gas feeds from the bottom, and the gas after CO2 is absorbed is discharged by the top.The CO2 collector may be seated on the electronic scale and (also can weigh by off-line), describes the time history curve of CO2 in the fermenting process by changes in weight.
Described solid-state tank top is equipped with temperature sensor, detects the fermentation bed and exports meteorological temperature, and this temperature sensor is connected with controller, by controller feedback control chuck water coolant magnetic valve and humidifier bypass magnetic valve.
The jar of described closed solid-state fermentation reaction device has chuck, and Jacket inlet is provided with magnetic valve, when needing cooling, by the controller feedback magnetic valve is opened, the feeding water coolant.
Described air humidifying system is provided with bypass, and bypass is opened by solenoid control, and the tank top temperature sensor detects temperature when too high, by the controller feedback, magnetic valve is switched, and the freezing air after the degerming can directly enter fermentor tank, assists heat radiation.
Water atomizing nozzle is equipped with in described solid state fermentation tank top, is connected with controller, and the interlock of the magnetic valve of its switch and humidification system bypass, shower nozzle starts 0-10 time/hour when the freezing air short circuit enters bed, and each minor tick 0-1 minute, 0.5-5 second at every turn.
Described closed solid-state fermentation reaction device also comprises the semiautomatic controls system, also can preestablish the time and the cycle of stirring in the fermentor tank and water spray and regulate water temperature in the humidifier according to different strain.
The present invention discloses a kind of Eco-power semi-automatic closed solid-state fermentation reaction device on the basis of CN101496574A, adopt oppositely two ribbon stirring rakes, material produces bidirectional-movement under oppositely two ribbon effects, solve the problem of sticking together when stirring solid substances, strengthened the blended effect.The vertical tail gas outlet of solid-state fermentation installation connects emptying and CO2 collector respectively, determines CO2 content in the tail gas by weighting method, shows the process and the fermentation appearance of fermentation with the variation of CO2 content.Solid-state fermenter has been set up chuck, and humidifier has increased bypass, and to Controlling System, the magnetic valve of adjustable clamp jacket water and humidifier gas bypassing is regulated the fermentation bed temperature automatically by jar upper thermocouple feedback information.The cycle of the temperature of humidifier and stirring and spray and time can preestablish according to the feature of different material fermentation, shift out heat of fermentation by chuck water coolant, the multiple modes such as freezing air, spray atomized water, stirring that feed, solve the most scabrous fermentation peak period bed overtemperature problem in the solid state fermentation.
Description of drawings
Fig. 1 closed solid-state fermentation reaction device agent structure synoptic diagram of the present invention.
Fig. 2 reverse pair of ribbon stirring rake of the present invention.
Fig. 3 inlet system synoptic diagram of the present invention.
Fig. 4 variation of the fermenting process CO2 respiratory intensity of collector acquisition of the present invention with process.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.Should be understood that following examples only are used to the present invention to be described but not to be used to limits the scope of the invention.
Solid-state fermentation reaction device of the present invention comprises solid-state fermenter, inlet system, humidification system and exhaust gas analysis system.
As shown in Figure 1, described solid-state fermenter is a closed container, is preferably a stainless steel column-shaped barrel.Described closed container center is provided with a central shaft 13, is fixed on the top ends of cans, and the upper end that stretches out in top cover is connected with motor 14 by belt 1, and hypomere is connected with two ribbons, forms stirring rake 6.As shown in Figure 2, described pair of ribbon stirring rake is made of two different ribbons of the opposite diameter of the hand of spiral, and ribbon width δ is the 1/10-1/30 of the diameter of described closed container, and ribbon diameter D is the 2/5-4/5 of the diameter of described closed container.Driven by motor stirring rake 6 rotates solid reactant is stirred, article one, ribbon promotion material moves upward, and another ribbon promotes material and moves downward, and makes material form bidirectional-movement, axially and radially mixing, avoiding material attached to blade or jar top.Starter motor 14 can make mixing speed be controlled at 0-15rpm, thereby satisfy the slow-revving requirement of thread thalline solid state fermentation after regulating by speed change, potentiometer.
Also be provided with fermentation tail gas outlet 5 on the described closed container top cover, one is used for (being connected with controller to the inner atomizer 12 that sprays moisture of closed container, can manually be provided with, also can be) by setting in advance and the interlock of the magnetic valve on controller road, one is used to measure the temperature electrode 2 (being connected with controller) of described closed container top gas phase temperature, the one humidity electrode 3 and that is used to measure described closed container top gas phase humidity is used to measure the temperature electrode 4 of solid state reaction material bed temperature, when needs are measured the solid substances bed temperature, temperature electrode 4 is inserted in the reaction mass by top cover, and this temperature electrode 4 need withdraw from bed in advance during stirring.
Tail gas on described top cover outlet 5 is connected with CO2 collector 36 with evacuated tube by threeway, is controlled by valve 35.Under the fermentation normality, emptying is often opened, and closes emptying valve 35 when needing to analyze, and gas is imported moisture trap, the CO2 collector 36 of dress NaOH solution in entering then, and the NaOH strength of solution is 0.1-1M, aeration time is 2-20min.Obtain the CO2 increment by weighing and change, to understand fermentation appearance.
Place bottom closing on, described closed container inside is provided with a dispersion plate 8, be separated out an air chamber 11 in the lower end of described closed container, this air chamber is provided with inlet mouth 10, described inlet system links to each other with described inlet mouth 10, in closed container, feed preserve moisture gas or freezing air during the fermentation, gas enters air chamber 11 from bottom air inlet 10, enters the fermentation bed through behind the dispersion plate 8, then flows out from the air outlet 5 at closed container top.These air chamber 11 gas inside temperature adopt a thermometer 9 to measure.
In addition, described closed container outer wall also is provided with chuck 32, by the closure of controlled chilling water lines 33,34, comes regulating tank body temperature degree.
As shown in Figure 3, the inlet system of closed solid-state fermentation reaction device of the present invention comprises air compressor 15, filter membrane 16,17, snifting valve, gas meter 18, operated pneumatic valve 20,19, water storage bottle 22 and humidifier 23.Air compressor 15 connects gas meter 18 and connects humidifier 23, bypass 37 and water storage bottle 22 respectively by operated pneumatic valve 20,19, humidifier 23 links to each other with the air chamber 11 of described closed container with bypass 37, water storage bottle 22 links to each other with the atomizer 12 of described closed container, and described gas meter 18 both sides are respectively equipped with filter membrane 16,17, for security consideration, between gas meter 18 and filter membrane 16,17, be provided with snifting valve.
In inlet system of the present invention, after gas is compressed by air compressor 15, filter by filter membrane 16, air flowmeter 18 is regulated flow velocity, further filters through another filter membrane 17 again, is divided into two-way then, by the solenoid control that connects controller, one the tunnel directly enters pot bottom air chamber 11, and another road enters encloses container bottom air chamber 11 after heating humidifications through humidifier 23.When not needing to add moisture, the operated pneumatic valve 20 of humidifier one tunnel often is in to be opened the stage.In whole fermentation process, air compressor machine 15 is under the operating state always, when needing to stop to ventilate when sampling or on the technology, at this moment needs to open air-outlet valve and loses heart.Described humidifier 23 is provided with bypass 37 between the import and export pipeline, when the bed overtemperature, move towards by the solenoid control gas circuit.
Water in the humidifier 23 is provided by the liquid level difference by water storage bottle 21, flows to from humidifier 23 water-ins.Water is by heater heats, and temperature and water temperature that humidifier intermediary temperature electrode is used to measure water are stabilized in set(ting)value by the two-way temperature controller of heating cooling.When temperature surpasses set(ting)value, open bypass 37 magnetic valves, gas does not pass through humidifier 23, and directly enters fermentor tank.When fermentation ends, humidifier 23 internal water should in time be discharged by water outlet.Humidifier 23 has liquidometer can observe inner liquid level, prevents that water level is low excessively, influences the humidification effect.
When system's overtemperature, at first switching gas circuit makes freezing air enter bed, and need add moisture this moment to tank body in good time, gas valve 20 of short duration the closing of leading to tank body, simultaneously, open the operated pneumatic valve 19 of waterworks, gas leads to water storage bottle 22, sterilized water in the bottle is pressed to fermentor tank, by atomizer 12, spray moisture (0-10 time/hour, 0.5-5 second) at every turn.Meanwhile open and stir, make mixing of materials even.
Further, solid-state fermentation reaction device of the present invention also comprises the semiautomatic controls system, by stirring and the water spray in time and the periodic Control fermentor tank is set.Control realizes by the time relay, sets in advance time length and interval that the time relay is opened, the running of control electrode, thereby opening time that control is stirred and interval, and mixing speed is 1-10r/min, churning time is 1-10min.Water spraying time and interval also are like this.Two-way control temperature controller also is set, water temperature in the control humidifier in the Controlling System.
When using closed solid-state fermentation reaction device of the present invention, earlier solid-state fermenter and material are sterilized, use liquid state fermentation pyrosphere method commonly used during inoculation, access solid seed.Adopt the sterilization of humidifier wet-hot steam method for the sterilization of humidifier and pipeline.In the time of need adding moisture to material, adopt after filtration that the aseptic gas of membrane filtration is pressed into tank body with the sterilized water in the water storage bottle, become atomized water to be sprayed on the material, open simultaneously and stir, moisture is mixed.
Example:
In of the present invention one preferred enforcement, at first the fermentation solid material is placed in the jar, place jar and sterilized water water storage bottle in the Autoclave and under 121 ℃ of conditions, sterilize 30min.Add sterilized water to certain altitude to humidifier.Open the humidifier heater switch, sterilized water is heated to boiling and produces steam, continue heating 15min, make humidifier and gas pipeline reach sterilising effect.Behind the 15min, close heater switch, the humidifier temperature is set to leavening temperature, the humidifier colling end is connected with fan, and the humidifier cooling is opened, and opens air compressor simultaneously, makes gas pass through humidifier, quickens the cooling of humidifier.Treat the humidifier temperature-stable when leavening temperature, the humidifier air outlet be connected with the encloses container inlet mouth that gas enters solid-state fermenter.Open the inoculation mouth, under aseptic technique, add the solid seed.Open and stir, seed and solid materials are mixed.Behind the 15min, close stirring, begin fermentation.
In the microbial fermentation stage, show the fermentation situation by the temperature and humidity electrode.Prescribe a time limit when temperature surpasses on the microbial fermentation, strengthen ventilation flow rate, the gas short circuit enters bed, and the chuck water coolant is opened, and stirring at low speed is opened, and the spray atomized water is with the reduction temperature.When opening waterworks, stage is closed the gas operated pneumatic valve that leads to jar, simultaneously, opens the operated pneumatic valve of waterworks, and gas leads to the water storage bottle presses to the tank body shower nozzle with the sterilized water in the bottle, sprays after making water atomization.The gas circuit that enters jar is opened in the spray end again, and the time of stirring and water spray and cycle can be controlled by the time relay is manual or automatic.
The closed container top is provided with the tail gas outlet, be connected with the CO2 collector with evacuated tube by threeway, monitoring CO2 increment changes, and understands fermentation appearance, the fermenting process CO2 respiratory intensity that collector obtains effectively solves the CO2 problem analysis in the fermenting process with the variation of process as shown in Figure 4.
Claims (6)
1. closed solid-state fermentation reaction device, comprise solid-state fermenter, it is characterized in that, described solid-state fermenter is the container of a confining zone chuck, described container band atomizer, dispersion plate, temperature electrode, humidity electrode, whipping appts and CO2 collector, described whipping appts is oppositely two ribbon stirring rakes, described reverse pair of ribbon stirring rake is made of two ribbons that the hand of spiral is opposite, diameter is different, the ribbon width is the 1/10-1/30 of the diameter of described closed container, and the ribbon diameter is the 2/5-4/5 of the diameter of described closed container.
2. solid-state fermentation reaction device as claimed in claim 1, it is characterized in that, fermentation tail gas outlet on the described solid state fermentation top ends of cans connects threeway, be connected with the CO2 collector with evacuated tube, described CO2 collector is one the container of sodium hydroxide solution to be housed easily, dried fermentation tail gas feeds from the bottom, gas after CO2 is absorbed is discharged by the top, described CO2 collector may be seated on the electronic scale or by off-line weighs, and describes the time history curve of CO2 in the fermenting process by the weightening finish of CO2 collector.
3. closed solid-state fermentation reaction device as claimed in claim 1 is characterized in that, described solid state fermentation tank wall also is provided with chuck, and Jacket inlet is provided with magnetic valve, and when leavening temperature was too high, water coolant was gone in the valve opening solenoid unblank.
4. closed solid-state fermentation reaction device as claimed in claim 1 is characterized in that described air humidifying system is provided with bypass, when the fermentation bed temperature is too high, switches by magnetic valve, and the freezing air after the degerming can directly enter fermentor tank.
5. closed solid-state fermentation reaction device as claimed in claim 1 is characterized in that the atomizer of described solid-state fermenter is connected with controller, its switch and the interlock of described magnetic valve, and shower nozzle starts when the freezing air short circuit enters bed.
6. closed solid-state fermentation reaction device as claimed in claim 1 is characterized in that described device also comprises the semiautomatic controls system, is used to preestablish time of stirring in the fermentor tank and water spray and cycle, and regulates water temperature in the humidifier.
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| CN2011100869846A CN102199521A (en) | 2011-04-07 | 2011-04-07 | Enclosed solid state fermentation bioreactor |
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| CN2011100869846A CN102199521A (en) | 2011-04-07 | 2011-04-07 | Enclosed solid state fermentation bioreactor |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583850A (en) * | 2012-08-17 | 2014-02-19 | 广州市纽溢乐商贸有限公司 | Preparation method of composite microecologics, and fermenting equipment of preparation method |
| CN105777239A (en) * | 2016-05-24 | 2016-07-20 | 安徽瑞丝环保能源有限公司 | Biological fertilizer fermentation tank |
| CN107950753A (en) * | 2017-12-28 | 2018-04-24 | 广东省农业科学院动物科学研究所 | A kind of method of aerobic-anaerobic coupled fermentation production biological feedstuff |
| CN108220139A (en) * | 2018-03-22 | 2018-06-29 | 云南师范大学 | A kind of difference position double helix stirring integratedization solid fermentation device |
| CN110157613A (en) * | 2019-05-23 | 2019-08-23 | 湖南民康生物技术研究所 | A kind of layer frame endless track formula solid state fermentation equipment and process for solid state fermentation |
| CN110238170A (en) * | 2019-07-09 | 2019-09-17 | 三门踱哒环保设备有限公司 | A kind of degradation reaction pond degraded using microorganism to rubbish |
| CN114075507A (en) * | 2021-11-18 | 2022-02-22 | 山东泰山生力源集团股份有限公司 | Laboratory anaerobic fermentation equipment and anaerobic fermentation method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583850A (en) * | 2012-08-17 | 2014-02-19 | 广州市纽溢乐商贸有限公司 | Preparation method of composite microecologics, and fermenting equipment of preparation method |
| CN105777239A (en) * | 2016-05-24 | 2016-07-20 | 安徽瑞丝环保能源有限公司 | Biological fertilizer fermentation tank |
| CN107950753A (en) * | 2017-12-28 | 2018-04-24 | 广东省农业科学院动物科学研究所 | A kind of method of aerobic-anaerobic coupled fermentation production biological feedstuff |
| CN107950753B (en) * | 2017-12-28 | 2024-02-06 | 广东省农业科学院动物科学研究所 | Method for producing biological feed by aerobic and anaerobic coupled fermentation |
| CN108220139A (en) * | 2018-03-22 | 2018-06-29 | 云南师范大学 | A kind of difference position double helix stirring integratedization solid fermentation device |
| CN108220139B (en) * | 2018-03-22 | 2020-09-18 | 云南师范大学 | Differential double-screw stirring integrated solid fermentation device |
| CN110157613A (en) * | 2019-05-23 | 2019-08-23 | 湖南民康生物技术研究所 | A kind of layer frame endless track formula solid state fermentation equipment and process for solid state fermentation |
| CN110157613B (en) * | 2019-05-23 | 2022-08-26 | 湖南民康生物技术研究所 | Layer-frame circulating crawler-type solid-state fermentation equipment and solid-state fermentation method |
| CN110238170A (en) * | 2019-07-09 | 2019-09-17 | 三门踱哒环保设备有限公司 | A kind of degradation reaction pond degraded using microorganism to rubbish |
| CN110238170B (en) * | 2019-07-09 | 2020-07-03 | 浙江昆仑环保设备有限公司 | Degradation reaction tank for degrading garbage by using microorganisms |
| CN114075507A (en) * | 2021-11-18 | 2022-02-22 | 山东泰山生力源集团股份有限公司 | Laboratory anaerobic fermentation equipment and anaerobic fermentation method |
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Application publication date: 20110928 |