CN102199056A - Method for prolonging storage life of compound microbial fertilizer - Google Patents
Method for prolonging storage life of compound microbial fertilizer Download PDFInfo
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- CN102199056A CN102199056A CN2010105885319A CN201010588531A CN102199056A CN 102199056 A CN102199056 A CN 102199056A CN 2010105885319 A CN2010105885319 A CN 2010105885319A CN 201010588531 A CN201010588531 A CN 201010588531A CN 102199056 A CN102199056 A CN 102199056A
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- microbiological fertilizer
- composite microbiological
- molasses
- fertilizer
- wheat bran
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Abstract
The invention relates to the microbial field, and specifically relates to a method for prolonging the storage life of a compound microbial fertilizer. The storage life of the compound microbial fertilizer is prolonged by adding molasses and wheat bran into the compound microbial fertilizer, wherein the added amount of molasses and wheat bran equals 2% to 5% of the total compound microbial fertilizer amount by weight. According to the invention, the molasses and wheat bran are added into the compound microbial fertilizer, such that the microbial vitality is sustained. With the cooperation of the added components, the storage life of the compound microbial fertilizer can be prolonged.
Description
Technical field
What the present invention relates to is microorganism field, a kind of specifically method that prolongs the composite microbiological fertilizer preservation period.
Background technology
Microbial fertilizer is the particular product that a class contains live microorganism, and present studies show that, vinelandii, genus bacillus, photosynthetic bacterium and cellulose-decomposing bacteria, milk-acid bacteria, yeast, actinomycetes etc. all have function in plant production.Microbial fertilizer is applied in the agriculture production, can obtain specific fertilizer effect, and wherein live microorganism plays a key effect, owing to contain microbial activities factor alive in the microbial fertilizer, can promote plant-growth.Therefore, the long-term maintenance of effective microbe vigor is a key point of keeping the microbial fertilizer long-term effectiveness in the microbial fertilizer.
At present, the principal element that the restriction microbial fertilizer is promoted is that the effective microbe quantity of microbial fertilizer is low, and effect descends further under the prolonged preservation condition.The protective material that exploitation prolongs the microbial fertilizer preservation period is one of effective means that addresses this problem.
Summary of the invention
The purpose of this invention is to provide a kind of method that prolongs the composite microbiological fertilizer preservation period.
For achieving the above object, the technical solution used in the present invention is:
A kind of method that prolongs the composite microbiological fertilizer preservation period: molasses and wheat bran are added in the composite microbiological fertilizer, and then prolong the preservation period of composite microbiological fertilizer; Wherein, the addition of molasses and wheat bran is 2% ~ 5% of a composite microbiological fertilizer gross weight.
The mass ratio of described molasses and wheat bran is 1: 5-1: 4.Described molasses are the concentrated molasses after the beet sugar manufacture.
Described composite microbiological fertilizer is:
1) multiplication culture of composite bacteria: with Candida utilis, streptococcus acidi lactici, Lactobacterium acidophilum, subtilis, slow raw soybean root nodule bacterium, give birth to the fat azospirillum, have a liking for swamp Rhodopseudomonas, bacillus megaterium, colloid bacillus cereus and azotobacter chroococcum respectively by weight percentage the inoculum size of 1%-4% in substratum separately, carry out multiplication culture respectively, until logarithmic phase;
2) Candida utilis, streptococcus acidi lactici, Lactobacterium acidophilum, subtilis co-fermentation are cultured to total count and reach 10
9More than, with the slow raw soybean root nodule bacterium that are cultured to logarithmic phase respectively, give birth to the fat azospirillum, have a liking for swamp Rhodopseudomonas, bacillus megaterium, colloid bacillus cereus and azotobacter chroococcum, account for 40% according to the co-fermentation thing, single culturing bacterium accounts for 10% mixed respectively, obtains complex microorganism;
3) composite microbiological fertilizer: the bacterial classification that above-mentioned increment is respectively cultivated mixes, then will mix the back bacterial classification is inoculated in the liquid fermentation medium according to weight percent 4%, under 30 ℃, fermentation culture 70h, fermented product pH value is 4.5-7.5, probiotics total amount in every milliliter of substratum reaches more than 3,000,000,000, obtains composite microbiological fertilizer.
]
1. the prolongation method of composite microbiological fertilizer preservation period of the present invention is that molasses and wheat bran are added in the composite microbiological fertilizer, can make the vigor of microorganism all keep effect, by adding and being used, can in the preservation process, prolong the preservation period of composite microbiological fertilizer;
2. interpolation molasses and wheat bran can make the preservation period of composite microbiological fertilizer can extend to more than 12 months among the present invention; And the material that the present invention adds is easy to obtain, and is with low cost.
Embodiment
Embodiment 1
1) increment of composite bacteria is cultivated: with Candida utilis, streptococcus acidi lactici, Lactobacterium acidophilum and subtilis respectively by weight percentage 3% inoculum size in substratum separately, carry out multiplication culture respectively, until logarithmic phase;
2) with the above-mentioned Candida utilis of multiplication culture respectively, streptococcus acidi lactici, Lactobacterium acidophilum, subtilis 1: 1: 1 by volume: 1 mixes, then will mix the back bacterial classification is inoculated in the liquid fermentation medium according to weight percent 4%, under 30 ℃, fermentation culture 70h, fermented product pH value please be write clearly for also having other requirements in the 4.5-7.5(culturing process), probiotics total amount in every milliliter of substratum reaches more than 3,000,000,000, wherein liquid fermentation medium is a glucose 2%, peptone 1%, extractum carnis 1%, yeast extract paste 0.5%, surplus is a distilled water, then with tunning respectively with slow raw soybean root nodule bacterium, give birth to the fat azospirillum, swamp Rhodopseudomonas and bacillus megaterium mix, tunning accounts for 40%, slow raw soybean root nodule bacterium account for 10%, give birth to the fat azospirillum and account for 10%, swamp Rhodopseudomonas accounts for 10%, bacillus megaterium accounts for 10%, colloid bacillus cereus account for 10% and azotobacter chroococcum account for 10% fermented product that obtains complex microorganism after mixing.
Above-mentioned bacterial strains all can be respectively cultivated according to ordinary method, and a kind of in the numerous culture condition of the knowledge that present embodiment provides is respectively:
The substratum of Candida utilis: potato is soaked powder 3g, glucose 20g, and agar powder 15g, distilled water adds to 1000mL, 121 ℃ of autoclavings 20 minutes.Candida utilis multiplication culture condition is cultivated 24-48h for 28 ℃.
The substratum of streptococcus acidi lactici: glucose 2%, peptone 1%, extractum carnis 1%, yeast extract paste 0.5%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, sal epsom 0.058%, manganous sulfate 0.025%, agar 1.5-2%, pH6.5.121 ℃ of autoclavings 20 minutes.Streptococcus acidi lactici multiplication culture condition is cultivated 48-72h for 30 ℃.
The substratum of Lactobacterium acidophilum: glucose 2%, peptone 1%, extractum carnis 1%, yeast extract paste 0.5%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, sal epsom 0.058%, manganous sulfate 0.025%, agar 1.5-2%, pH6.5.121 ℃ of autoclavings 20 minutes.Lactobacterium acidophilum multiplication culture condition is cultivated 48-72h for 37 ℃.
The substratum of subtilis: peptone 10g, agar 20g, sodium-chlor 5g, meat soak powder 10g, and water adds to 1000mL, pH7.2.121 ℃ of autoclavings 20 minutes.Subtilis multiplication culture condition is cultivated 48-72h for 30 ℃.
The substratum of slow raw soybean root nodule bacterium: N.F,USP MANNITOL 10g, K
2HPO
40.5g, yeast powder 0.4g, MgSO
47H
2O0.2g, NaCl0.1g, CaCl
26H
2O0.1g, distilled water 1000mL, pH6.8-7.0.121 ℃ of autoclavings 20 minutes.Slow raw soybean root nodule bacterium multiplication culture condition is cultivated 48-72h for 28 ℃.
Give birth to the substratum of fat azospirillum: sucrose 10g, K
2HPO
40.5g, NaCl0.1g, yeast extract paste 1g, ironic citrate (1%) 1mL, CaSO
40.2g, MgSO
47H2O0.2g, MnSO
4(1%) 1mL, NaMoO
4(1%) 1mL, boric acid (1%) 1mL, distilled water 1000mL, pH6.8-7.0; 121 ℃ of autoclavings 20 minutes.Give birth to fat azospirillum multiplication culture condition under 25-30 ℃ of condition, cultivate 48-72h.
Have a liking for the substratum of swamp Rhodopseudomonas: yeast powder 0.3%, peptone 0.3%, sal epsom 0.05%, calcium chloride 0.03%, distilled water 1000mL, pH6.8-7.0; 121 ℃ of autoclavings 20 minutes.Have a liking for swamp Rhodopseudomonas multiplication culture condition under 30 ℃ of conditions, cultivate 72-96h.
The substratum of bacillus megaterium: peptone 0.5%, extractum carnis 0.3%, sodium-chlor 0.5%, distilled water 1000mL, pH7.0; 121 ℃ of autoclavings 20 minutes.Bacillus megaterium multiplication culture condition is cultivated 24-48h under 30 ℃ of conditions.
Colloid bacillus cereus substratum: sucrose 10g, yeast extract paste 0.2g, (NH
4) SO
40.5g, MgSO
40.1g, KCl0.1g, Na
2HPO
40.1g, CaCO
31.0g, distilled water 1000mL, pH7.0.121 ℃ of autoclavings 20 minutes; The multiplication culture condition is cultivated 20-40h under 32-36 ℃ of condition.
Azotobacter chroococcum substratum: glucose 10g, KH
2PO
40.2g, MgSO
47H
2O0.2g, NaCl0.2g, CaSO
47H
2O0.2g, CaCO
35.0g, distilled water 1000mL, pH7.0-7.2 sterilized 30 minutes for 113 ℃.The multiplication culture condition is cultivated 24-48h under 28-36 ℃ of condition.
Above-mentioned bacterial strains is all available from Chinese agriculture microbial strains preservation center, and cultivates separately according to prior art well known to those of ordinary skill in the art, and its Candida utilis is in the ACCC20060 that is numbered at Chinese agriculture microbial strains preservation center; Streptococcus acidi lactici is in the ACCC10653 that is numbered at Chinese agriculture microbial strains preservation center; Lactobacterium acidophilum is in the CICC6005 that is numbered at Chinese industrial microbial strains preservation center; Subtilis is in the CICC20037 that is numbered at Chinese industrial microbial strains preservation center; Slow raw soybean root nodule bacterium are in the ACCC15402 that is numbered at Chinese agriculture microbial strains preservation center; Give birth to the fat azospirillum in the ACCC10481 that is numbered at Chinese agriculture microbial strains preservation center; Swamp Rhodopseudomonas is in the ACCC10649 that is numbered at Chinese agriculture microbial strains preservation center; Bacillus megaterium is in the ACCC02991 that is numbered at Chinese agriculture microbial strains preservation center; Colloid bacillus cereus is in the ACCC02983 that is numbered at Chinese agriculture microbial strains preservation center; Azotobacter chroococcum is in the ACCC10098 that is numbered at Chinese agriculture microbial strains preservation center.
3) molasses and wheat bran are added in the composite microbiological fertilizer, mix, add carrier, 20-40 ℃ of cryodrying (can suitably select 20 ℃, 30 ℃, 35 ℃ or 40 ℃), and then the preservation period that prolongs composite microbiological fertilizer; The addition of molasses and wheat bran is 5% of a composite microbiological fertilizer gross weight, and the mass ratio of wherein said molasses and wheat bran is 1: 5, and wherein, molasses are the byproduct of beet sugar manufacture, is a kind of thickness, chocolate, is semifluid object.
The above-mentioned composite microbiological fertilizer that is added with molasses and wheat bran is preserved in 25 ℃ of constant temperature, and colony counting method is measured the bacterium number (referring to table 1) in the composite microbiological fertilizer of preserving different times.
Table 1 adds the influence of protective material to the composite microbiological fertilizer preservation period
Embodiment 2
1) increment of composite bacteria is cultivated: with Candida utilis, streptococcus acidi lactici, Lactobacterium acidophilum and subtilis respectively by weight percentage 1% inoculum size in substratum separately, carry out multiplication culture respectively, until logarithmic phase;
2) Candida utilis, streptococcus acidi lactici, Lactobacterium acidophilum, subtilis co-fermentation are cultured to total count and reach 10
9More than, with the slow raw soybean root nodule bacterium that are cultured to logarithmic phase respectively, give birth to the fat azospirillum, have a liking for swamp Rhodopseudomonas, bacillus megaterium, colloid bacillus cereus and azotobacter chroococcum, account for 40% according to the co-fermentation thing, single culturing bacterium accounts for 10% mixed respectively, obtains composite microbiological fertilizer.
3) molasses and wheat bran are added in the composite microbiological fertilizer, mix, add carrier, 20-40 ℃ of cryodrying, and then the preservation period that prolongs composite microbiological fertilizer; The addition of molasses and wheat bran is 2% of a composite microbiological fertilizer gross weight, and wherein, the mass ratio of described molasses and wheat bran is 1: 4.
Claims (4)
1. a method that prolongs the composite microbiological fertilizer preservation period is characterized in that: molasses and wheat bran are added in the composite microbiological fertilizer, and then prolong the preservation period of composite microbiological fertilizer; Wherein, the addition of molasses and wheat bran is 2% ~ 5% of a composite microbiological fertilizer gross weight.
2. by the method for the described prolongation composite microbiological fertilizer of claim 1 preservation period, it is characterized in that: the mass ratio of described molasses and wheat bran is 1: 5-1: 4.
3. by the method for the described prolongation composite microbiological fertilizer of claim 1 preservation period, it is characterized in that: described molasses are the concentrated molasses after the beet sugar manufacture.
4. by the method for the described prolongation composite microbiological fertilizer of claim 1 preservation period, it is characterized in that: described composite microbiological fertilizer is:
1) multiplication culture of composite bacteria: with Candida utilis, streptococcus acidi lactici, Lactobacterium acidophilum, subtilis, slow raw soybean root nodule bacterium, give birth to the fat azospirillum, have a liking for swamp Rhodopseudomonas, bacillus megaterium, colloid bacillus cereus and azotobacter chroococcum respectively by weight percentage the inoculum size of 1%-4% in substratum separately, carry out multiplication culture, until logarithmic phase;
2) Candida utilis, streptococcus acidi lactici, Lactobacterium acidophilum, subtilis co-fermentation are cultured to total count and reach 10
9Individual/more than the mL, with the slow raw soybean root nodule bacterium that are cultured to logarithmic phase respectively, give birth to the fat azospirillum, have a liking for swamp Rhodopseudomonas, bacillus megaterium, colloid bacillus cereus and azotobacter chroococcum, account for 40% according to the co-fermentation thing, single culturing bacterium accounts for 10% mixed respectively, obtains complex microorganism;
3) composite microbiological fertilizer: the bacterial classification that above-mentioned increment is respectively cultivated mixes, then will mix the back bacterial classification is inoculated in the liquid fermentation medium according to weight percent 4%, under 30 ℃, fermentation culture 70h, fermented product pH value is 4.5-7.5, probiotics total amount in every milliliter of substratum reaches more than 3,000,000,000, obtains composite microbiological fertilizer.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103553814A (en) * | 2013-11-08 | 2014-02-05 | 苏州仁成生物科技有限公司 | Nano sustained-release fertilizer prepared by multiple fermentation and preparation method thereof |
| CN103553838A (en) * | 2013-11-08 | 2014-02-05 | 苏州仁成生物科技有限公司 | Insecticidal and bacteriostatic biofertilizer and preparation method thereof |
| CN103588586A (en) * | 2013-10-10 | 2014-02-19 | 苏州仁成生物科技有限公司 | Microbial fertilizer and preparation method thereof |
| CN103588551A (en) * | 2013-10-10 | 2014-02-19 | 苏州仁成生物科技有限公司 | Multifunctional microbial fertilizer and preparation method thereof |
| CN103601564A (en) * | 2013-10-10 | 2014-02-26 | 苏州仁成生物科技有限公司 | Preparation method of microbial fertilizer |
| CN109896900A (en) * | 2019-04-26 | 2019-06-18 | 沈阳华生生物科技开发有限公司 | A kind of preparation and application of complex micro organism fungicide and biological organic complex fertilizer |
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| CN1490397A (en) * | 2002-10-19 | 2004-04-21 | 珠海科恩生物工程有限公司 | Preservation of living bacteria in biological products by corn starch |
| CN101717301A (en) * | 2009-11-12 | 2010-06-02 | 北京理工大学 | Capsule type microbial fertilizer and preparation method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103588586A (en) * | 2013-10-10 | 2014-02-19 | 苏州仁成生物科技有限公司 | Microbial fertilizer and preparation method thereof |
| CN103588551A (en) * | 2013-10-10 | 2014-02-19 | 苏州仁成生物科技有限公司 | Multifunctional microbial fertilizer and preparation method thereof |
| CN103601564A (en) * | 2013-10-10 | 2014-02-26 | 苏州仁成生物科技有限公司 | Preparation method of microbial fertilizer |
| CN103588551B (en) * | 2013-10-10 | 2015-12-02 | 顾祥茂 | A kind of multifunctional microbial fertilizer and preparation method thereof |
| CN103588586B (en) * | 2013-10-10 | 2016-01-27 | 绥化学院 | A kind of microbial fertilizer and preparation method thereof |
| CN103553814A (en) * | 2013-11-08 | 2014-02-05 | 苏州仁成生物科技有限公司 | Nano sustained-release fertilizer prepared by multiple fermentation and preparation method thereof |
| CN103553838A (en) * | 2013-11-08 | 2014-02-05 | 苏州仁成生物科技有限公司 | Insecticidal and bacteriostatic biofertilizer and preparation method thereof |
| CN103553814B (en) * | 2013-11-08 | 2016-02-17 | 武汉日清生物科技有限公司 | One utilizes multiple fermentation to prepare Nano sustained-release fertilizer and preparation method thereof |
| CN109896900A (en) * | 2019-04-26 | 2019-06-18 | 沈阳华生生物科技开发有限公司 | A kind of preparation and application of complex micro organism fungicide and biological organic complex fertilizer |
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Application publication date: 20110928 |