CN102197752A - Method for planting indigowoad root - Google Patents
Method for planting indigowoad root Download PDFInfo
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- CN102197752A CN102197752A CN2011101205645A CN201110120564A CN102197752A CN 102197752 A CN102197752 A CN 102197752A CN 2011101205645 A CN2011101205645 A CN 2011101205645A CN 201110120564 A CN201110120564 A CN 201110120564A CN 102197752 A CN102197752 A CN 102197752A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/12—Technologies relating to agriculture, livestock or agroalimentary industries using renewable energies, e.g. solar water pumping
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Abstract
The invention discloses a method for planting indigowoad root, which comprises the following processes of: (1) soil preparation; (2) culture of seedlings, wherein the survival rate of culturing the seedlings is improved by optimizing and filtering the seedlings through a chemical method; (3) field management; and (4) harvesting and processing, wherein indigowoad leaves are harvested in lunar November in the same year of planting isatis tinctoria, overground parts are cut out, collected and sun-dried and roots are dug out to be sun-dried after the indigowoad leaves are harvested after 7 to 14 days. By the method for planting the indigowoad root, the yield and the quality of the indigowoad root can be improved.
Description
Technical field
The present invention relates to the kind method for planting of Radix Isatidis
Background technology
Radix Isatidis Radix lsatidis is the dry root of cruciferae isatis Isatis indigotica Fort., is clearing heat and detoxicating conventional Chinese medicine, and normal and other Chinese medicine composition compound is general to be used for multiple disease such as influenza, encephalitis, hepatitis etc.
Woaded blue is biennial vegetation, is distributed in ground such as China Inner Mongol, Shaanxi, Gansu, Hebei, Shandong, Jiangsu, Zhejiang, Anhui, Guizhou, and is normal for cultivating.
The source difference of Radix Isatidis is used as Chinese medicine, also there are differences, and then shows as the difference on the drug effect clinically.This is relevant with concrete production environment and cultivation method, therefore, how to obtain the Radix Isatidis of high-quality, is the target that woaded blue plant personnel and enterprise are pursued.
Summary of the invention
Purpose of the present invention just is to provide a kind of cultivation method of Radix Isatidis, the Radix Isatidis that utilizes this method to obtain, and quality is higher, has better effect aspect the endotoxemia being used to prevent and treat.
The present invention adopts following technical scheme: a kind of cultivation method of Radix Isatidis, and comprise whole ground, grow seedlings, field management and gathering, it is characterized in that:
When growing seedlings, earlier ' ' the C mass concentration is that 24% potassium permanganate was soaked seed 11～13 hours to C-50 with 40 with the woaded blue seed, pull out and mix fine sand after drying or ash broadcasts sowing: when gathering, folium isatidis (leaf of woaded blue) is gathered at woaded blue cultivation lunar calendar then November, choosing is fine, prescinds acrial part, dries after the collection: behind the folium isatidis of gathering 7～" day uproot; remove clean reed head and earth, dry.
Comparative optimization be, to select 3 years or the above husky territory of not planting Radix Isatidis and having irrigation condition during whole ground, plough deeply 25-35 centimetre, use 1500～2000 kilograms of the compost that becomes thoroughly decomposed or barnyard manure for every mu, or every mu apply humic acid biological fertilizer 100-120 kilogram, furrow are made in thin, the leveling of rake then.
Described field management process can comprise: carry out thinning and fill the gaps with seedlings in the time of height of seedling 6-10 centimetre, carry out intertill and clean tillage simultaneously, Feng Hanghou forbids weeding; Arid is in time watered; Should note the ditch cleaning draining rainy season, prevents mashed root of ponding and introduced disease between furrow; Process of growth ten dispenser prevention and elimination of disease and pests: impose fertilizer 1-3 time.
Radix Isatidis (root of woaded blue) with this method acquisition of the present invention, best in quality, adopt endotoxin to cause rabbit fever models and investigate its correlation effect, show through test, the medicinal extract high and low dose group of using the Radix Isatidis extraction of this method cultivation can significantly suppress the rapid rising of time body temperature index of Response TR14, reduce the degree (family＜0.05 or P＜0.01) of endotoxin pyrogenic effect, the antiendotoxin activity intensity that adopts this method can improve Radix Isatidis is described, has better quality.
Embodiment
The invention will be further described below in conjunction with specific embodiment, to help understanding content of the present invention.
One, embodiment 1
Cultivation place: White Cloud Mountain, Fuyang Radix Isatidis company of Hutchison China Trade Holdings cultivation base: (1) choosing ground and whole ground: selected 3 years or the above husky territory of not planting Radix Isatidis and having irrigation condition, the tractor-ploughing in time of preceding crop results back is ploughed deeply about 30 centimetres, use through fully becoming thoroughly decomposed for every mu, meet 1500～2000 kilograms of the compost of sanitary standard or barnyard manure, also can every mu apply " humic acid biological fertilizer " 100-120 kilogram, thin, the leveling of rake then, make wide about 2 meters, 25 centimetres of furrow widths, high 25 centimetres furrow.
(2) grow seedlings: clear and bright to carrying out around grain rains.Before the sowing with seed with 40 ' C-50 ' C3% potassium permanganate was soaked seed 12 hours, standby to carry out vernalization and sterilization, to pull out drying, mix fine sand or ash and can broadcast sowing.Uniform broadcasting is in smooth good plot, with 3-5 centimetre of rake general ± covering.2 kilograms of every mu of sowing quantities are broadcast the back and can be emerged in 5-10 days.
(3) field management: carry out thinning and fill the gaps with seedlings seeding row spacing in the time of height of seedling 6-10 centimetre: be about 5～7 centimetres X20 centimetre.Look careless growing state is in time intertilled, weeding, Feng Hanghou forbids weeding.Impose fertilizer in vegetative period 1～3 time, 25 kilograms every mu of humic acid biological fertilizers evenly apply in the middle of the line-spacing, plough under in the soil during intertillage.Arid is in time watered, can be before at 10 in the morning, pour water after at 4 in afternoon, if water under the exposure in sunshine at noon, and the blade of easily burning.Should note the ditch cleaning draining rainy season, prevents mashed root of ponding and introduced disease between furrow.
Vegetative period the extermination of disease and insect pest in the following way:
1., downy mildew: the main harm blade, also can endanger petiole.Ten back sides of their early stage mouth produce white or canescence mustiness thing, and the very not tangible yellow-white scab in border takes place on the blade face.Send out rear blade flavescence gradually, it is withered and dead to be brown at last.Their early stage is sprayed with 1: 1: 200 bordeaux mixture, or with 600 times of liquid of 60% zineb or 500 times of liquid mists of 50% carbendazim.Spray in per 7 days 1 time connects and sprays 2-3 time.
2., stalk break: this disease can endanger complete stool, by soil infection.Morbidity season spray medicine protection: use grey sulphur mixture in the plant base portion; Their early stage is doubly sprayed with 65% zineb 500-600, every spray in 7 days once, and 2-3 time continuously.Or with 50% carbendazim, topsin 500-1000 times liquid, soup is concentrated and is sprayed the plant middle and lower part, generally every spray in 7-10 days once.
8., root rot: period of disease is watered the diseased plant root with 50% topsin 800-1000 times liquid.
4., white blister: their early stage sprays bordeaux mixture (1: 1: 120), every 7 days 1 time, sprays 2-3 time continuously.
5., black spot: their early stage, spray bordeaux mixture (1: 1: 120) or 65% zineb 600-800 times liquid, every spray in 10～15 days 1 time, every mu of consumption 75kg sprays 2-3 time continuously.500 times of liquid of the also available 50% metalaxyl wetting powder of their early stage spray.
6., imported cabbageworm (cabbage caterpillar): the 5-6 month is endangered blade.The method of control is that instar larvae is sprayed every mu of consumption 20-30 gram with cabbage caterpillar particle virus Bt composite insecticide; Or with bacillus thuringiensis wetting powder or the spraying of Ke Li $00-1000 times of liquid: or prevent and treat with 1000 times of liquid sprayings of 90% chlorophos crystal flolimat.Diamond-back moth: the same cabbage caterpillar of the method for preventing and treating.
7., three-spotted plusia: every mu sprays 2-2.5kg2.5% chlorophos pulvis.
8., aphid: the tender stem of main harm, leaf and the bud of just having extracted out.With 40% dimethoate emulsifiable concentrate 1500-2000 times of liquid spraying.
(4), gather and process:
1, gathering of folium isatidis: collection claims to dry behind the fresh weight.Gather November in year.Choosing is fine, prescinds acrial part, the gathering of root: uprooted in 7-14 days behind the folium isatidis of gathering, remove clean reed head and earth
Two, Comparative Examples:
In order to verify whether cultivation method of the present invention influences the quality of the Radix Isatidis product of acquisition (root of woaded blue), carried out the contrast cultivation in same plot, adopted traditional cultivation method, be included in before the breeding with 40 ℃-50 ℃ emerge in worm water seed 12 hours with the difference of embodiment 1, gathering of root is to gather simultaneously with folium isatidis.
Three, contrast experiment's example
For the quality of scientific evaluation Radix Isatidis, in conjunction with its product clinical indication, consider drug activity judging quota in the body emphatically, adopt endotoxin to cause rabbit fever models and investigate its correlation effect.
1. material and instrument
1.1 medicine
Radix Isatidis extractum A: adopt Radix Isatidis (root of the woaded blue) medicinal material that cultivation method obtains among the embodiment 1, press the preparation of banlangen granules, banlangen keli extraction process.
Radix Isatidis extractum B: adopt the Radix Isatidis (root of woaded blue) of cultivation method (conventional method) acquisition of Comparative Examples, press the preparation of banlangen granules, banlangen keli extraction process.
Radix Isatidis extractum C: press the preparation of banlangen granules, banlangen keli extraction process.
Aspirin enteric coated tablet.
It is an amount of to get Radix Isatidis extractum A, B, adds an amount of distilled water respectively, and mixing is made and is equivalent to that crude drug in whole is 2.0,1.0, the soup of 0.5Z/mL; Radix Isatidis extractum C makes with method and is equivalent to
Crude drug in whole is the soup of 1.0g/mL, and is standby.
1.2 animal
Japan large ear rabbit, male and female are regardless of, body weight 1.8-2.5kg.
1.3 reagent
Lipopolysaccharides (LPS, 5mg, Sigma company, L-3755); It is pure that all the other reagent are analysis.
2. method and result
Select healthy rabbits, survey anus temperature every day 2 times, 3d continuously progressively adapts to it: body temperature should be in 38.0-39.6 ℃, and body temperature fluctuates and is no more than 0.3 ' C.According to animal body
The pre-result that measures of temperature filters out 72 of the qualified rabbit of body temperature, is divided into 9 groups at random, 8 every group.The high, medium and low dosage group of medicine respectively by 6.0,3.0,1.5g.kg-' irritates stomach and gives medicine,
Model group gives with volume distilled water, and positive controls gavages aspirin with 200rog-kg-'.
Except that positive controls, all the other respectively organize every mortar administration 1 time, continuously 5d.1.5h after the last administration measures the rabbit basal body temperature, totally 3 times, gets its average.Behind the 1h, auricular vein injection LPS 200ng.kg-', measure rabbit body temperature in endotoxin attack back 0.5,1,2,3,6h, observe its situation of change, and compare with basal body temperature, calculating maximum temperature difference (AT), time body temperature index of Response (TR14 tries to achieve area under the temperature time curve with trapezoidal method), is to suppress percentage according to calculating heating with TR14.
Data are handled: the factor that influences the endotoxin pyrogenic reaction experiment is numerous, mainly comprise laboratory animal general state (body weight, spirit, appetite, excreta, active situation etc.), experimental situation (temperature, humidity) etc., especially the rabbit basal body temperature should remain in the 38.0-39.6 ℃ of scope, and the highest and minimum mensuration body temperature differs and is no more than 0.4 ℃ of person and can includes basis in.
Because in the experiment detailed process, exist the part animal because of general state, room temperature change, disturbance excessively, the influences such as action interference of noise and experiment operator, cause its temperature deviation to some extent, these type of data are all disallowable.On initial data (n=8) basis, reject some body temperature and do not meet relevant data and the animal individual (rejecting 2 data for every group) of including standard code in,
Medicine group and model group are carried out the f check relatively, the results are shown in Table 1.
The influence of table 1 pair endotaxin induction fever in rabbits effect
Group dosage number of animals AT (' C) the TR14 inhibiting rate
Model group
The aspirin group
Radix Isatidis A high dose
Dosage among the Radix Isatidis A
Radix Isatidis A low dosage
Radix Isatidis B high dose
Dosage among the Radix Isatidis B
Radix Isatidis B low dosage
Dosage among the Radix Isatidis C
1.14～0.30
1.07～0.36
1.02～0.33
1.06～0.42
0.8710.34
1.05+0.23
1.13+0.47
1.28+0.27
1.05m0.39
3.67～1.20
2.51～1.44
1.06～0.84**
2.55～1.42
1.88～1.57*
2.85～1.56
3.39～1.12
4.21～0.93
3.12～1.05
Annotate: compare * P＜0.05 with model group " P＜0.01
31.64％
71.06％
30.58％
48.90％
22.31％
7.65％
——14.72％
15.06％
By table 1 result, compare with model group, Radix Isatidis A medicinal extract high and low dose group can significantly suppress the rapid rising of time body temperature index of Response TR14, reduces the degree (corpse＜0.05 or P＜0.01) of endotoxin pyrogenic effect.But each group is to the reduction degree of maximum temperature difference AT and model group unknown significance difference relatively, shows that its intensity to the fever in rabbits effect of endotaxin induction do not have significance and influence.From the heating inhibiting rate, Radix Isatidis B medicinal extract presents dose-dependent inhibition endotoxin heating effect, and Radix Isatidis A medicinal extract does not have significant correlation, and suppressing activity intensity is Radix Isatidis A＞Radix Isatidis C＞Radix Isatidis B.
Conclusion: (" Radix Isatidis A medicinal extract can significantly suppress the fever in rabbits effect of endotaxin induction, and its activity intensity is higher relatively; (2) Radix Isatidis has certain antagonistic effect for the degree (TR14) of endotaxin induction fever in rabbits effect, but for its calorific intensity (AT) control ability slightly a little less than; (3) from the antiendotoxin effect analysis, there is some difference between the Radix Isatidis different batches product of White Cloud Mountain, but its quality product (Radix Isatidis A) effect intensity is significantly higher than the market like product.That is, adopt method cultivation Radix Isatidis of the present invention, quality is better, should include GAP cultivation standard in, carries out the industrialization production of Radix Isatidis.
Claims (4)
1. the cultivation method of a Radix Isatidis comprises whole ground, grows seedlings, field management and gathering, and it is characterized in that:
When growing seedlings, is that 6-8% potassium permanganate was soaked seed 15～20 hours with 44 ℃ of-60 ℃ of mass concentrations earlier with the woaded blue seed, pull out and mix fine sand after drying or ash broadcasts sowing;
When gathering, folium isatidis is gathered at woaded blue cultivation lunar calendar then November, and choosing is fine, prescinds acrial part, receives and dries; Behind the folium isatidis of gathering, uprooted in 7-14 days, remove clean reed head and earth, dry.
2. the cultivation method of Radix Isatidis as claimed in claim 1, it is characterized in that described whole ground process is: selected 3 years or above husky territory of not planting Radix Isatidis, plough deeply 25-35 centimetre, use compost or the barnyard manure 1000-2000 kilogram that becomes thoroughly decomposed for every mu, or every mu apply humic acid biological fertilizer 120-180 kilogram, furrow are made in thin, the leveling of rake then.
3. the cultivation method of Radix Isatidis as claimed in claim 1 is characterized in that described field management process comprises:
Carry out thinning and fill the gaps with seedlings in the time of height of seedling 8-10 centimetre, carry out intertill and clean tillage simultaneously, Feng Hanghou forbids weeding;
Arid is in time watered, and should note the ditch cleaning draining rainy season, prevents mashed root of ponding and introduced disease between furrow;
Dispenser prevention and elimination of disease and pests in the process of growth;
Impose fertilizer 1-3 time.
4. to state the process of watering be to carry out before at 10 in the morning, after at 4 in afternoon to the cultivation breeding method of Radix Isatidis as claimed in claim 3.
It is characterized in that: carry out before described at 10 in the process morning of watering and after at 4 in afternoon.
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| CN2011101205645A CN102197752A (en) | 2011-05-10 | 2011-05-10 | Method for planting indigowoad root |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498847A (en) * | 2011-10-19 | 2012-06-20 | 南京农业大学 | Screening method of advantage-based stock seed of isatis root |
| CN102696382A (en) * | 2012-06-15 | 2012-10-03 | 山西省农业科学院经济作物研究所 | Indigowoad root suitable for sprout production and method for growing indigowoad root sprouts |
| CN102763544A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Low-cost method for planting Dyer's woad in sandy lands |
| CN102763542A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in loam lands |
| CN102763543A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting isatis roots on clay land parcels at low cost |
| CN103098587A (en) * | 2012-10-09 | 2013-05-15 | 东北林业大学 | Technology of planting woads in saline-alkali field |
| CN104488484A (en) * | 2014-11-18 | 2015-04-08 | 师宗广朋农业科技有限公司 | Pollution-free cultivation method for isatis root |
| CN105027912A (en) * | 2015-07-20 | 2015-11-11 | 江苏农牧科技职业学院 | Cultivation method for interplanting officinal cape jasmines and radix isatidis |
| CN105474963A (en) * | 2015-12-15 | 2016-04-13 | 安徽省康君食品有限公司 | Radix Salviae Miltiorrhizae planting method |
| CN105493844A (en) * | 2015-12-15 | 2016-04-20 | 安徽省康君食品有限公司 | Isatis tinctoria planting method |
| CN109769677A (en) * | 2019-03-22 | 2019-05-21 | 福建省中科生物股份有限公司 | A kind of plant factor's Radix Isatidis ciltivating process |
| CN113331202A (en) * | 2021-05-06 | 2021-09-03 | 中国中药有限公司 | Isatis root seed coating agent, coating method and application thereof |
-
2011
- 2011-05-10 CN CN2011101205645A patent/CN102197752A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498847B (en) * | 2011-10-19 | 2013-04-03 | 南京农业大学 | Screening method of advantage-based stock seed of isatis root |
| CN102498847A (en) * | 2011-10-19 | 2012-06-20 | 南京农业大学 | Screening method of advantage-based stock seed of isatis root |
| CN102696382A (en) * | 2012-06-15 | 2012-10-03 | 山西省农业科学院经济作物研究所 | Indigowoad root suitable for sprout production and method for growing indigowoad root sprouts |
| CN102763544B (en) * | 2012-06-30 | 2014-03-19 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in sandy lands |
| CN102763544A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Low-cost method for planting Dyer's woad in sandy lands |
| CN102763542A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in loam lands |
| CN102763543A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting isatis roots on clay land parcels at low cost |
| CN103098587A (en) * | 2012-10-09 | 2013-05-15 | 东北林业大学 | Technology of planting woads in saline-alkali field |
| CN104488484A (en) * | 2014-11-18 | 2015-04-08 | 师宗广朋农业科技有限公司 | Pollution-free cultivation method for isatis root |
| CN105027912A (en) * | 2015-07-20 | 2015-11-11 | 江苏农牧科技职业学院 | Cultivation method for interplanting officinal cape jasmines and radix isatidis |
| CN105474963A (en) * | 2015-12-15 | 2016-04-13 | 安徽省康君食品有限公司 | Radix Salviae Miltiorrhizae planting method |
| CN105493844A (en) * | 2015-12-15 | 2016-04-20 | 安徽省康君食品有限公司 | Isatis tinctoria planting method |
| CN109769677A (en) * | 2019-03-22 | 2019-05-21 | 福建省中科生物股份有限公司 | A kind of plant factor's Radix Isatidis ciltivating process |
| CN113331202A (en) * | 2021-05-06 | 2021-09-03 | 中国中药有限公司 | Isatis root seed coating agent, coating method and application thereof |
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Application publication date: 20110928 |