CN102191297A - Method for making tricholoma matsutake polysaccharides - Google Patents
Method for making tricholoma matsutake polysaccharides Download PDFInfo
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- CN102191297A CN102191297A CN2011100847739A CN201110084773A CN102191297A CN 102191297 A CN102191297 A CN 102191297A CN 2011100847739 A CN2011100847739 A CN 2011100847739A CN 201110084773 A CN201110084773 A CN 201110084773A CN 102191297 A CN102191297 A CN 102191297A
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- mycelium
- blazei polysaccharide
- tricholoma matsutake
- matsutake
- dried
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the field of development of edible fungus resources and particularly relates to a method for extracting tricholoma matsutake polysaccharides from tricholoma matsutake mycelium obtained through liquid fermentation. The method comprises the steps of: weighing 10g of cane sugar, 1g of yeast extract, 5mg of calcium chloride, 100mg of ammonium tartrate, 0.05mg of nicotinic acid, 0.05mg of folic acid, 0.5mg of ferric citrate, 0.45mg of zinc sulfate, 0.5mg of manganese sulfate, 100mg of monopotassium phosphate and 50mg of magnesium sulfate, dissolving in 100ml of water, stirring to reach a uniform state, sterilizing for 30min at 121 DEG C, cooling to 20 DEG C, inoculating, culturing for 9 days at 20-24 DEG C in a shaker at the speed of 150rpm, filtering fermentation liquor to obtain the tricholoma matsutake mycelium, and drying the mycelium until the water content of the mycelium is below 10% to obtain dry mycelium; crushing the dry mycelium to 20 meshes, adding water 20 times more than the crushed dry mycelium, reacting for 80min at 65 DEG C to extract polysaccharides; and decoloring by using activated carbon, centrifuging to remove precipitates, adding 95% ethyl alcohol into supernate for alcohol precipitation, centrifuging to obtain precipitates, drying until the water content of the precipitates is below 10% to obtain a crude product of the tricholoma matsutake polysaccharides.
Description
(1), technical field
The invention belongs to domestic fungus resource development technique field, specifically from the tricholoma matsutake mycelium that liquid fermenting obtains, extract the method for blazei polysaccharide.
(2), background technology
Matsutake has another name called Trichotoma matsutake, is famous and precious edible mushrooms.Its unique flavor, mouthfeel is lubricious.High resilience, the food back is left a lingering fragrance in one's mouth, and is bright fragrant peculiar.Matsutake has SOD activity improving, quickens the removing of free radical, delays the histoorgan decline, improves cardiovascular function, enhances metabolism, and the raising human body is antiviral, anti-cell suddenlys change and the ability of increase immunologic function.The generation of Tricholoma matsutake (lto et lmai) Singer sporophore and growth phase are when difficulty, and the generation of bacterium bed forms by the Nature fully, and output is extremely low, though the artificial culture of Tricholoma matsutake (lto et lmai) Singer sporophore is not succeedd yet through making great efforts for many years.Artificial culture can only prepare the mycelium of matsutake at present, owing to comprise that many nutritive ingredients of blazei polysaccharide are very approaching in mycelium and the sporophore, so can utilize tricholoma matsutake mycelium exploitation blazei polysaccharide product, realize the matsutake Sustainable utilization of resources.
(3), summary of the invention
The objective of the invention is to present situation, the making method of a kind of low cost, high-quality blazei polysaccharide is provided at wild matsutake inadequate resource.
The object of the present invention is achieved like this: carry out the cultivation of tricholoma matsutake mycelium earlier, carry out the extraction of blazei polysaccharide again.Take by weighing sucrose 10 grams, yeast extract paste 1 gram, 5 milligrams in calcium chloride, 100 milligrams of ammonium tartrates, 0.05 milligram in nicotinic acid, 0.05 milligram in folic acid, 0.5 milligram of ironic citrate, 0.45 milligram in zinc sulfate, 0.5 milligram of manganous sulfate, 100 milligrams of potassium primary phosphates, 50 milligrams in sal epsom is dissolved in 100 ml waters, stirs, sterilized 30 minutes for 121 ℃, be cooled to 20 ℃, insert seed, 20-24 ℃, rotary shaker 150rpm cultivated 9 days.Cultivate to finish the after-filtration fermented liquid and get tricholoma matsutake mycelium, then tricholoma matsutake mycelium is dried to moisturely below 10% under 50-60 ℃ of condition, promptly get the dried mycelium of matsutake.The dried mycelium powder of matsutake is broken to 20 orders, add 20 times of water, 65 ℃ of temperature, the reaction times is that 80min extracts lentinan, decolours 40-50 minute for 65 ℃ with the gac insulation of 0.3%-0.5% then, the centrifugal precipitation of removing, add 95% ethanol in the supernatant liquor, making ethanol final volume mark is 75%, the centrifuging and taking precipitation, under 50-60 ℃ of condition, be dried to moisturely below 10%, promptly get the lentinan crude product.
(4), specific embodiments
Carry out the cultivation of tricholoma matsutake mycelium earlier, carry out the extraction of blazei polysaccharide again.The cultural method of tricholoma matsutake mycelium is: take by weighing sucrose 10 grams, yeast extract paste 1 gram, 5 milligrams in calcium chloride, 100 milligrams of ammonium tartrates, 0.05 milligram in nicotinic acid, 0.05 milligram in folic acid, 0.5 milligram of ironic citrate, 0.45 milligram in zinc sulfate, 0.5 milligram of manganous sulfate, 100 milligrams of potassium primary phosphates, 50 milligrams in sal epsom is dissolved in 100 ml waters, stirs, sterilized 30 minutes for 121 ℃, be cooled to 20 ℃, insert the matsutake bacterial classification, 20-24 ℃, rotary shaker 150rpm cultivated 9 days.Cultivate to finish the after-filtration fermented liquid and get tricholoma matsutake mycelium, then tricholoma matsutake mycelium is dried to moisturely below 10% under 50-60 ℃ of condition, promptly get the dried mycelium of matsutake.
The dried mycelium powder of matsutake is broken to 20 orders, take by weighing 5 grams and add 100 ml waters, 65 ℃ of temperature, reaction times is that 80min extracts lentinan, adds 65 ℃ of decolourings of 0.5 gram gac insulation 45 minutes then, the centrifugal precipitation of removing, add 375 milliliters of 95% ethanol in the supernatant liquor, centrifuging and taking precipitation is dried to moisturely below 10% under 50-60 ℃ of condition, promptly gets the lentinan crude product.
Claims (5)
1. the making method of a blazei polysaccharide is characterized in that: carry out the cultivation of tricholoma matsutake mycelium earlier, carry out the extraction of blazei polysaccharide again; Take by weighing sucrose 10 grams, yeast extract paste 1 gram, 5 milligrams in calcium chloride, 100 milligrams of ammonium tartrates, 0.05 milligram in nicotinic acid, 0.05 milligram in folic acid, 0.5 milligram of ironic citrate, 0.45 milligram in zinc sulfate, 0.5 milligram of manganous sulfate, 100 milligrams of potassium primary phosphates, 50 milligrams in sal epsom is dissolved in 100 ml waters, stir, sterilized 30 minutes for 121 ℃, be cooled to 20 ℃, insert seed, 20-24 ℃, rotary shaker 150rpm cultivated 9 days, cultivated end after-filtration fermented liquid and got tricholoma matsutake mycelium, under 50-60 ℃ of condition, be dried to tricholoma matsutake mycelium moisture below 10% then, promptly get the dried mycelium of matsutake, the dried mycelium powder of matsutake is broken to 20 orders, adds 20 times of water gagings, 65 ℃ of temperature, reaction times is that 80min extracts blazei polysaccharide, then with 65 ℃ of decolourings of 0.3-0.5% gac insulation 40-50 minute, the centrifugal precipitation of removing, add 95% (v/v) ethanol in the supernatant liquor, making ethanol final volume mark is 75% (v/v), and centrifugal 10 minutes of 3000rpm gets precipitation, under 50-60 ℃ of condition, be dried to moisturely below 10%, promptly get the blazei polysaccharide crude product.
A kind of blazei polysaccharide according to claim 1 making method, it is characterized in that: the dried mycelium of matsutake that makes is broken to 20 orders, adds 20 times of water, 65 ℃ of temperature, the reaction times is that 80min extracts blazei polysaccharide.
A kind of blazei polysaccharide according to claim 1 making method, it is characterized in that: the aqueous solution behind the water extraction need be with 65 ℃ of decolourings of 0.3-0.5% gac insulation 40-50 minute.
A kind of blazei polysaccharide according to claim 1 making method, it is characterized in that: centrifugal 10 minutes of the aqueous solution 3000rpm after the decolouring, add 95% ethanol in the supernatant liquor, making ethanol final volume mark is 75%, the centrifuging and taking precipitation.
A kind of blazei polysaccharide according to claim 1 making method, it is characterized in that: the centrifugation behind the alcohol precipitation, under 50-60 ℃ of condition, be dried to moisturely below 10%, be the blazei polysaccharide crude product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011100847739A CN102191297A (en) | 2011-04-06 | 2011-04-06 | Method for making tricholoma matsutake polysaccharides |
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| CN2011100847739A CN102191297A (en) | 2011-04-06 | 2011-04-06 | Method for making tricholoma matsutake polysaccharides |
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| CN2011100847739A Pending CN102191297A (en) | 2011-04-06 | 2011-04-06 | Method for making tricholoma matsutake polysaccharides |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102885291A (en) * | 2012-11-01 | 2013-01-23 | 黑龙江省麒麟工贸公司 | Tricholoma-matsutake chewing tablet and preparation method thereof |
| CN104223287A (en) * | 2013-06-22 | 2014-12-24 | 何寒 | Pleurotus polysaccharide beverage and preparation method of pleurotus polysaccharide beverage |
| CN104286828A (en) * | 2014-09-24 | 2015-01-21 | 杜超峰 | Tricholoma matsutake vitamin composition, and preparation methods of tricholoma matsutake alcohols and tricholoma matsutake polysaccharides |
| CN111248026A (en) * | 2020-04-07 | 2020-06-09 | 中国科学院沈阳应用生态研究所 | Quercus matsutake culture medium and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102885291A (en) * | 2012-11-01 | 2013-01-23 | 黑龙江省麒麟工贸公司 | Tricholoma-matsutake chewing tablet and preparation method thereof |
| CN104223287A (en) * | 2013-06-22 | 2014-12-24 | 何寒 | Pleurotus polysaccharide beverage and preparation method of pleurotus polysaccharide beverage |
| CN104286828A (en) * | 2014-09-24 | 2015-01-21 | 杜超峰 | Tricholoma matsutake vitamin composition, and preparation methods of tricholoma matsutake alcohols and tricholoma matsutake polysaccharides |
| CN111248026A (en) * | 2020-04-07 | 2020-06-09 | 中国科学院沈阳应用生态研究所 | Quercus matsutake culture medium and application thereof |
| CN111248026B (en) * | 2020-04-07 | 2022-04-08 | 中国科学院沈阳应用生态研究所 | Quercus matsutake culture medium and application thereof |
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Application publication date: 20110921 |