CN102172145A - Application of waste compost microbial agent to improving salt resistance of lawn grass - Google Patents
Application of waste compost microbial agent to improving salt resistance of lawn grass Download PDFInfo
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Abstract
The invention discloses an application of a city domestic waste compost microbial agent to improving salt resistance of lawn grass. The composite microbial agent consists of three bacteria comprising bacillus subtilis, actinomycetes and saccharomycetes according to a volume ratio at 1:1:1, and the composite microbial agent is 100-200 folds diluted; the salt resistance refers to 0.3%-0.9% NaCl saline-alkali soils. The experimental result shows that: after two lawn plants are inoculated with the city domestic waste compost microbial agent, the maximum stress capacity for resisting salt stress can reach 0.9% of salt stress, so that the 100-200 folds of diluted composite microbial agent prepared by the invention obviously promotes the relieving of lawn plant salt stress.
Description
Technical field
The invention belongs to environmental protection technical field, relating to the probiotics that extracts in the producing fertilizer from refuse in daily life is raw material, preparation highly effective compost composite bacteria agent capable.The compost complex micro organism fungicide say so more specifically in the application that improves aspect the lawn plant salt-resistance.
Background technology
Composted (composting) is microorganism (bacterium, actinomycetes, fungi etc.) or the commercial strain that utilizes natural world extensively to exist, controlledly promote biodegradable organic matter to stable humus (humicsubstance, HS) Biochemical processes of Zhuan Huaing.This process also is the part of earth surface ecological process, and it participates in the matter and energy circulation of earth surface.
Under artificial controlled condition, the organic matter in the solid waste can be transformed into organic manure one compost (compost) by composted, this organic manure is as the end product of composted, not only stable performance, and very little to the harm of environment.Therefore, composted is a solid waste, especially one of effective way of innoxious, stabilisation of domestic waste and resource.Composted development has been experienced very long history so far, and the historical process of its generation and development is looked back and analyzed, and can be to develop new composting process technology from now on reference and help in the theory and practice are provided.
Old generation and etc. (1989) adopt the rubbish medium, in the producing fertilizer from refuse in daily life processing procedure, when temperature reaches 45 ℃ and 55 ℃, the compost bacterial strain is separated, and find in the time of 45 ℃: Aspergillus [Aspergillus], bacillus [Bacillus], Pseudomonas [Pseudomonas] and Sporolactobacillus [Sporolactobacillus] etc. are the dominant microfloras in the compost.Then different during to 55 ℃, except bacillus [Bacillus] and Pseudomonas [Pseudomonas] still are the dominant microflora, Lactobacillus [Lactobacillus], streptococcus [Streptococcus] and micromonospora [Micromonospora] belong to then becomes new dominant microflora, substituted other kind microorganism, show that in the composted process microbial population is among the dynamic equilibrium that constantly changes.
Isolate the microorganism of a large amount of participation high temperature aerobic compostings from domestic waste, be respectively bacillus, Pseudomonas through evaluation, Lactobacillus, staphylococcus, Escherichia etc. 5 belong to bacterium; Aspergillus, mucor, monascus, Penicillium, Geotrichum, mould genus, arteries and veins sporeling genus, cephalosporiums etc. 8 belong to mould; Streptomyces 1 belongs to actinomycetes; Blastocystis, fission yeast bacterium etc. 2 belong to saccharomycete.This result of study has effectively promoted research of microorganism in the garbage compost and utilization.Hassen(2001) the biological investigation that changes is found to city compost process Ei, the high temperature that decomposing organic matter produces causes the important change of microbiologic population, pathogenic bacteria, saccharomycete and mesophile are reduced in a large number, and sporiferous bacillus exists in a large number, in composting process, hot stage initial stage and Chinese stage bacterium are all in the highest flight.Marvin's ripples etc. has also been reported thermophilic fungal, and thermophilic brown actinomycetes etc. participate in the microbial population of garbage compost.
Organic composition from domestic waste, and decomposed utilization by Institute of Micro-biology.But various microorganisms are not quite similar to the capacity of decomposition and the decomposition rate of various materials, the microbial population that occurs in (middle temperature, high temperature) composting process under the different temperatures and quantitatively also completely different.
Recent pertinent literature and actual production show: how to improve at present technical merit that producing fertilizer from refuse in daily life handles, improve treatment effeciency, improve product quality, be the research contents that the related scientific research personnel are paid close attention to always; In compost material,, confirmed it is to accelerate compost maturity, improve the method for composting efficiency by a lot of research institutes at some artificial microorganism formulations of the autotelic interpolation of predetermined substance.Consider in a lot of researchs and production practices, the often more single bacterial strain of the composite bacteria agent capable of rational proportion has more good effect, so how the various function stems of extracting in the compost and screening are combined exploitation highly effective compost composite bacteria agent capable, fundamentally optimize with preparation rationally and use city compost matrix just to become the hot issue of related scientific research personnel's researchs.Although the effect study of plant microbe inoculation report is more, the microbial bacterial agent that most of research work relates to is only limited to from soil and extracts.Consumer garbage compostization is microorganism (bacterium, actinomycetes, fungi etc.) or the commercial strain that utilizes natural world extensively to exist, controlledly promoting biodegradable organic matter to the Biochemical processes that stable humus transforms, is to be realized reclaiming organic waste, innoxious dynamic process by the very fast a plurality of micropopulation actings in conjunction of structure of community succession.
Studies show that, single bacterium, fungi, actinomycetes colony, no matter how high its activity is, and the effect in accelerating the composted process is all less than the acting in conjunction of complex microbial community.People such as Ros and P é rez-Piqueres have studied the influence of inoculation inoculating microbe composite bacteria agent capable to microorganism in the composted process, help understanding the Biochemical processes of composting process and adding the influence of microorganism formulation to compost.Chinese scholars has been carried out series of theories and practical studies to the microorganism phenomenon in the composting process in recent years, people such as Vaz-Moreira analyze the community diversity of bacterium in the compost, in the hope of deeply being familiar with the essence of composting process, for the research of composting technology and technology is provided fundamental basis.But in the complex micro organism fungicide research process, evaluation and classification about the inner microbe species of compost, symbiosis and the relation of influencing each other have ambiguity and complexity between the compost bacterial classification, and the compost microbe microbial inoculum is applied under the normal condition the aspect that influences to plant growing, correlative study is also bright to be report.And about the evaluation and the classification of the inner microbe species of compost, symbiosis and the relation of influencing each other have ambiguity and complexity between the compost bacterial classification, and from producing fertilizer from refuse in daily life, extract screening and the preparation compost is compounded with the beneficial microorganism microbial inoculum, the research that influences the aspect of lawn plant growth, physiological property under the salt stress be yet there are no report.
Summary of the invention
The producing fertilizer from refuse in daily life complex micro organism fungicide that the present invention will be mixed with variable concentrations is inoculated in the turf establishment system.Test is slight, moderate and severe salt stress carry out under handling, complex micro organism fungicide by the research variable concentrations is to the influence of perennial ryegrass and Festuca Arundinacea physiological and ecological characteristic, purpose is for screening good anti-salt compost complex micro organism fungicide, improve the lawn plant salt-resistance, promote the planting of salt stress environment lawn plant that foundation is provided.
For achieving the above object, the invention provides following technical scheme:
The application of producing fertilizer from refuse in daily life complex micro organism fungicide aspect raising lawn plant salt-resistance, complex micro organism fungicide wherein is a bacillus subtilis for dilution 100-200 volume ratio doubly: 3 bacterial classifications of actinomycetes: saccharomycete=1:1:1 are formed; Described salt refers to 0.3%-0.9%NaCl saline-alkali soil.
Application of the present invention, lawn plant wherein are perennial ryegrass or Festuca Arundinacea.
Application of the present invention, wherein said salt-resistance refers to: the compost microbe microbial inoculum is promoting the perennial ryegrass photosynthetic rate, is alleviating the application aspect the chlorophyll content decline that salt stress causes; Described complex micro organism fungicide is a bacillus subtilis for the volume ratio of 100 times of dilutions: 3 bacterial classifications of actinomycetes: saccharomycete=1:1:1 are formed.
Application of the present invention, wherein said salt-resistance refers to: the application of compost microbe microbial inoculum aspect enhancing root system of plant opposing salt stress; Described salt refers to 0.3%-0.9%NaCl saline-alkali soil.
Application of the present invention, wherein said salt-resistance refers to: the application of compost microbe microbial inoculum aspect increase Festuca Arundinacea chlorophyll content; Complex micro organism fungicide wherein is a bacillus subtilis for the volume ratio of 200 times of dilutions: 3 bacterial classifications of actinomycetes: saccharomycete=1:1:1 are formed.
Embodiment
In order to explain enforcement of the present invention more fully, provide following preparation method's embodiment.These embodiments only are to explain rather than limit the scope of the invention.Its medium-height grass bacillus, actinomycetes, saccharomycete also can be prepared according to the method for embodiment 1 by commercially available.
Embodiment 1
1, the screening of bacterial strain
1. enrichment: the compost sample of gathering is taken by weighing 10 g place aseptic triangular flask, after adding the vibration evenly of 100 ml sterile waters and bead, get 10 ml suspension in the triangular flask that fills 100 ml enriched mediums, at 28 ℃, under 220 r/min shaken cultivation 3-5 days.2. primary dcreening operation: after the culture fluid after the enrichment carried out the concentration gradient dilution, coat on the separation screening medium, be inverted constant temperature culture, observe the size of transparent circle, choose transparent circle/colony diameter ratio and carry out multiple sieve than the bacterial strain of macrocolony.3. the drafting of growth curve: bacillus subtilis and saccharomycete that primary dcreening operation is obtained are inoculated in the corresponding shake-flask culture base, respectively at 37 ℃ and 28 ℃, cultivate under the condition of 220 r/min, and be ordinate with the OD value under wavelength 600 nm and 560 nm respectively, incubation time is an abscissa, draws the growth curve of bacterial strain.
2, complex micro organism fungicide preparation
Spawn culture: with bacillus subtilis, actinomycetes, the saccharomycete enlarged culture on corresponding medium of screening, concrete steps: 1. preserve the inclined-plane with the stroke-physiological saline solution washing, shake up to the aseptic triangular flask bacteria suspension; 2. inoculate slant culture in the seed bottle, bacillus subtilis is at 30 ℃, and 180r/min cultivates 12 h and obtains seed liquor, and actinomycetes and saccharomycete are at 28 ℃, and 220 r/min cultivate 48 h and obtain seed liquor; 3. the seed liquor of concentration 10% is inoculated into that enlarged culture 48 h obtain zymotic fluid in the fermentation flask, and composite microbial bacteria liquid is prepared (Ye Feng etc., 2009) by following processing.
Embodiment 2
Complex micro organism fungicide is used
Adopt the design of single factor randomized block experiment, limited resource is respectively: 1. salt stress, three salt stress processing horizontals; Slight (0.3%NaCl): T
1, moderate (0.6%NaCl): T
2And severe (0.9%NaCl) saline-alkali soil: T
32. the complex micro organism fungicide of variable concentrations is used, and sets processing mode under this condition of salt stress: handle 1 for contrast, do not inoculate microbial inoculum (CK); Handle the microbial inoculum (CM of 100 times of dilutions of 2 addings
1); Handle the microbial inoculum (CM of 200 times of dilutions of 3 addings
2); Handle 4 and add 300 times of dilution microbial inoculum (CM
3).
Embodiment 3 turf establishments
Select for use the more common English ryegrass of northern China (
Lolium perenneL.) and Festuca Arundinacea (
Festuca arundinaceaL.) be test material.For examination lawn plant seed full seed, size evenly.Used soil is garden mould in the institute of Tianjin Normal University, and its physicochemical property is: pH 7.44, saturation moisture content 0.58 ml/g, organic matter 4.68%, full nitrogen 0.21%, available phosphorus 22.03 mg/kg, full potassium 45.61g/kg.After the collection, reject foreign material such as plant undesirable root and chad, cross 2 mm sieve, at 121 ℃ of 30 min that sterilize down, standby.Potted plant method is adopted in experiment, places sterile soil 200 g in every culture vessel, and 0.4 g lawn seed is sowed on it.Unify quantitative water supply every day, water regime is preferably arranged, often change each culture vessel position, to guarantee the illumination unanimity to keep culture matrix.When the plant strain growth height reaches 2-3 cm, add the compost complex micro organism fungicide (CM) of variable concentrations.Each handles 3 repetitions, and experiment is cultivated on the platform hothouse plants and carried out, and temperature is 20 ~ 22 ℃, and relative moisture is 34% ~ 65%, and illumination is to penetrate indoor natural daylight.Handle the mensuration of carrying out each index of plant after 35 days.
Embodiment 4 testing indexs
(1) mensuration of soluble-salt concentration in the saline-alkali soil of Tianjin
Gravimetric method is adopted in the soluble-salt analysis: take by weighing 5 g samples respectively, put into 50 mL beakers.In beaker, put into 25 mL and do not have CO
2Distilled water stirs 3 min with glass bar, gets 100 mL beaker (W that weighed
1), filter with ready prepd filter.The time of placing is long more, and is big more to the errors of analytical results of solubility salinity, and therefore, filtration will be tried one's best rapidly and be experimentized as early as possible.On the beaker and filtrate dislocation thermostat water bath of having weighed, water bath method.In evaporate to dryness, yellow (existing organic in the residue) will remove organic matter (follow on a small quantity repeatedly principle, repeated treatments is till residue bleaches) with 15% hydrogenperoxide steam generator if residue is.Dry the beaker outer wall with filter paper then, put into baking oven, under 100-105 ℃ of condition, dry about 3-4 h to constant weight, weighing (W
2).Temperature is unsuitable too high during evaporate to dryness, in order to avoid spatter the mistake salinity, near evaporate to dryness the time, more will note spattering mistake, in order to avoid influence measurement result.The result calculates:
Plant height adopts each culture dish of centimeter scale chi to survey 5 plant heights at random.
The mensuration of ground and underground biomass.
The mensuration of chlorophyll content adopts spectrophotometric method.
The lawn plant acrial part of promptly getting 0.2g places mortar, add a small amount of quartz sand, calcium carbonate and 80% acetone, carry out abundant milling and extracting, homogenate is changed in the 15ml centrifuge tube, and with an amount of 80% washing with acetone alms bowl body, change in the lump in the centrifuge tube, abandon precipitation after centrifugal, get supernatant and be transferred in the 25ml volumetric flask, be settled to graduation mark with 80% acetone; With 80% acetone in contrast, produce the absorbance that UV-1700 type ultraviolet specrophotometer is determined at 663nm, 645nm wavelength place respectively with SHIMADZU.Computing formula is as follows:
In the formula, Ca is the concentration of chlorophyll a, and Cb is the concentration of chlorophyll b, C
TBe total chlorophyll concentration, unit is the mg/g dry weight.
(2) data analysis: adopt Microsoft Excel 2003 and SPSS 11.5 softwares to handle.
Bacterial screening
Primary dcreening operation:, obtain the big and bacterium colony faster of growing of transparent circle/colony diameter ratio by the plate isolation screening.With bacillus subtilis, actinomycetes, the saccharomycete of gained rule respectively separation, number and be preserved on the solid slant medium.
Multiple sieve: by measuring bacillus subtilis and saccharomycetic growth curve, analyze the highest and fertility of growth activity preferably period be logarithmic phase, the bacterial classification of this phase is extracted, be diluted to variable concentrations, be used for the lawn plant inoculation.By table 1 and as can be seen, be rich in bacillus subtilis, actinomycetes and saccharomycete in the gained microbial bacterial agent, the clump count of three bacterial classifications is 2.44 * 10
9-2.47 * 10
9Between, the OD of bacillus subtilis
600Value is 0.545, saccharomycetic OD
560Value is 0.567.
CM is to the influence of lawn plant ground biomass under the salt stress
The accumulation of salt stress obvious suppression perennial ryegrass ground biomass, through two-way analysis of variance, salt stress and compost complex micro organism fungicide (CM) all compare significantly (P＜0.05) to the influence of perennial ryegrass ground biomass.Along with the increase of salt stress degree, the accumulation of perennial ryegrass ground biomass significantly reduces.But after adding the compost complex micro organism fungicide, significantly increase the accumulation of perennial ryegrass ground biomass, alleviate because of salt stress and work the mischief.And be that CM effect under 100 is best with extension rate, slightly coerce down, be 2.16 times of contrast.Moderate coerce with severe coerce make respectively ground biomass be respectively the contrast 2.10 and 8.99 times.The compost complex micro organism fungicide of 200 and 300 multiples suppresses the perennial ryegrass ground biomass to salt stress and also has certain mitigation.
Under the situation of complex micro organism fungicide of inoculation, the Festuca Arundinacea ground biomass under the slight salt stress is than moderate salt stress and severe salt stress height, all have compared with the control significant difference (
P＜0.05), and be that the biomass of 200 compost complex micro organism fungicide accumulation is the highest with extension rate, under the slight salt stress, comparison is according to exceeding 56.95%.Moderate salt stress and severe salt stress are respectively 2.24 times and 3.86 times of contrast.
CM is to the influence of lawn plant underground biomass under the salt stress
The salt stress obvious suppression perennial ryegrass underground biomass accumulation, through two-way analysis of variance, salt stress and complex micro organism fungicide to the influence of perennial ryegrass underground biomass all compare significantly (
P＜0.05).Along with the increase of salt stress degree, the perennial ryegrass underground biomass is tangible downward trend, and after the inoculating complex microorganism microbial inoculum, downward trend is alleviated to some extent.When bacteria suspension concentration is 100 times of liquid, under slight and moderate salt stress and normal water supply all there were significant differences to the influence of perennial ryegrass underground biomass, be respectively 3.74 and 3.92 times of normal water supply, and the severe trend of coercing decreases.When complex micro organism fungicide was 200 times of liquid, moderate coerces to coerce with severe did not have remarkable influence to underground biomass, was significantly improved but compare all with normal water supply.300 diluted concentration DeGrains, but it influences to some extent to underground biomass accumulation.
When not inoculating the compost complex micro organism fungicide, salt stress makes the Festuca Arundinacea underground biomass descend to some extent.Under the situation that the salt stress degree strengthens gradually, along with the increase of complex micro organism fungicide extension rate, the Festuca Arundinacea underground biomass descends gradually, but all significant difference is arranged with contrast, under slight salt stress, best to dilute under 200 concentration gradients, be 3.80 times that contrast.Under the moderate salt stress, also being that 200 diluted concentration effects are best, is not inoculate 5.16 times of moderate salt stress under the microbial inoculum.Under the severe salt stress, 200 diluted concentration processing lists reveal higher underground biomass.
CM is to the influence of lawn plant root/shoot ratio under the salt stress
Salt stress and compost complex micro organism fungicide all compare significantly the influence of perennial ryegrass root/shoot ratio.Under slight salt stress situation, dilution times liquid is 100 complex micro organism fungicide, can obviously improve the perennial ryegrass root/shoot ratio, has improved 27.6% compared with the control.And under 200 and 300 dilution times liquid were handled, root/shoot ratio also increased, this explanation, and the compost complex micro organism fungicide helps promoting the underground biomass enrichment.Moderate is coerced the increase that has then stimulated root/shoot ratio, have compared with the control significant difference (
P＜0.05).Difference is not very remarkable and the complex micro organism fungicide of 100 and 300 diluted concentrations is coerced down in moderate and severe.
The Festuca Arundinacea root/shoot ratio is under slight salt stress situation, and along with the increase of compost complex micro organism fungicide diluted concentration descends gradually, the ratio under 200 processing is the highest, surpasses contrast 21.70% and contrast to produce notable difference.Under the situation of no inoculating complex microorganism microbial inoculum, root/shoot ratio may be the growth that has suppressed root system under the severe salt stress along with the increase of salt stress degree descends gradually, and after the inoculation microbial inoculum, root/shoot ratio all is to alleviate to some extent along with the increase of salt stress degree.Under moderate and the severe salt stress situation, all the root/shoot ratio of handling with 200 diluted concentrations is the highest.
CM is to the influence of lawn plant leaf chlorophyll under the salt stress
Chlorophyll is that plant carries out in the photosynthesis process important pigment that luminous energy is absorbed, transmits and transform, and the height of its content has tangible influence to photosynthesis of plants.The variation of perennial ryegrass chlorophyll content sees Table 2, and along with complex micro organism fungicide concentration increases, its content decreases chlorophyll a under slight salt stress situation, and diluted concentration is that 100 o'clock content is the highest, is 2.12 times of contrast.Do not have under the inoculating complex microorganism microbial inoculum situation, chlorophyll a content is along with the degree of coercing increases and reduces.Can obviously alleviate salt stress behind the inoculation compost complex micro organism fungicide to the synthetic negative effect that causes of chlorophyll a.The content of chlorophyll b reduces along with the increase of salt stress, behind the inoculation compost complex micro organism fungicide content minimizing that causes chlorophyll b because of salinity also there is tangible mitigation, the chlorophyll b content all has bigger variation under each bacteria suspension concentration, and is 100 to handle significant differences down with the microbial inoculum diluted concentration.The ratio of chlorophyll a/b also has significant variation under salt stress, under the situation that does not have the inoculating complex microorganism microbial inoculum, along with the increase of the degree of coercing, the ratio of chlorophyll a/b reduces thereupon; Under slight salt stress situation, reduced the ratio of chlorophyll a/b along with the increase of extension rate, it is the highest to be with extension rate that 100 concentration gradient is handled ratio, the ratio of the chlorophyll a/b under each concentration is handled is along with the increase of the degree of coercing has a declining tendency, may be under salt stress, to have suppressed chlorophyll a to synthesize, alleviate the influence of the conversion of luminous energy with the opposing salt stress.
Table 2 CM is to the influence (mgg of perennial ryegrass chlorophyll content under the salt stress
-1FW)
The inoculating complex microorganism microbial inoculum can obviously promote the chlorophyllous increase of Festuca Arundinacea (seeing Table 2), slight salt stress is handled down, after complex micro organism fungicide is handled, chlorophyll a significantly increases, the Festuca Arundinacea chlorophyll a content after the complex micro organism fungicide of different extension rates is handled all with contrast produce notable difference (
P＜0.05), chlorophyll a content is the highest under 200 diluted concentrations, is 2.78 times of contrast.Under the situation that does not have the inoculating complex microorganism microbial inoculum, increase along with the salt stress degree, chlorophyll a content descends to some extent, be 2.03 times that contrast under the severe salt stress, may be that Festuca Arundinacea increases photosynthesis in order to resist extraneous adverse circumstance under the salt stress, increase photosynthetic product and energy in the own body.Chlorophyll b content all has evident difference under each complex micro organism fungicide concentration processing and each salt stress.Total chlorophyll content, best to dilute 200 concentration treatment effects under slight salt stress, be 2.10 times that contrast, secondly be diluted concentration is 100 and 300, all produce notable difference with contrast.After complex micro organism fungicide is handled Festuca Arundinacea, the ratio of chlorophyll a/b reduces along with the increase of complex micro organism fungicide concentration, and the processing ratio that slight salt stress dilutes 200 concentration down is the highest, and comparison is according to exceeding 81.9%, and under this concentration, ratio reduces along with the increase of salt stress.Under moderate and the severe salt stress gradient, it is the most stable under 200 concentration that chlorophyll a/b ratio remains, and ratio compares higher.
Table 3 CM is to the influence (mgg of Festuca Arundinacea chlorophyll content under the salt stress
-1FW)
The development conclusion
The compost microbe microbial inoculum has obvious facilitation to the perennial ryegrass photosynthetic rate, can alleviate the chlorophyll content decline that salt stress causes, microbial inoculum dilutes 100 concentration treatment effect the bests, it is respectively 2.10 and 1.52 times of contrasts that slight and moderate is coerced down total chlorophyll content, also compares when severe is coerced according to exceeding 38.6%.Increase along with the salt stress degree, the content of perennial ryegrass leaf chlorophyll reduces gradually, and under the severe stress conditions, leaves of plants lamellar body inner chlorophyll content is minimum, and alleviated the adverse circumstance environment of plant after the inoculating complex microorganism microbial inoculum, have significant difference compared with the control.The perennial ryegrass ground biomass descends along with the increase of salt stress degree, and the microbial inoculum diluted concentration is 100 all the highest under each salt stress gradient, helps accumulating the perennial ryegrass ground biomass, is 2.16 times of contrast.Root/shoot ratio descends along with the increase of salt stress, and the compost complex micro organism fungicide is also inconsistent to the influence of perennial ryegrass root/shoot ratio under the different salt stress gradients, is slightly coercing down, and complex micro organism fungicide more has the enhancing of utilization root system of plant opposing salt stress to coerce.Complex micro organism fungicide can obviously increase the chlorophyll content of Festuca Arundinacea, with bacteria suspension concentration is that 200 o'clock treatment effects are best, under three kinds of salt stress levels, all show higher chlorophyll content, all with identical salt stress processing horizontal under contrast produce significant difference.Caused the reduction of Festuca Arundinacea ground and underground biomass under the salt stress, and rising along with the salt stress concentration gradient, accumulation reduces gradually, it is 200 o'clock at bacteria suspension concentration equally, Festuca Arundinacea has mitigation preferably under three kinds of salt stress levels, illustrate that inoculation compost complex micro organism fungicide has the inhibitory action of utilizing Festuca Arundinacea to alleviate salt stress, and after two kinds of lawn plants have inoculated the compost complex micro organism fungicide, the maximum ability of coercing of salt stress-resistant ability can reach 0.9% salt stress, illustrates that complex micro organism fungicide has facilitation to alleviating the lawn plant salt stress.
Claims (5)
1. the application of producing fertilizer from refuse in daily life compost complex micro organism fungicide aspect raising lawn plant salt-resistance, complex micro organism fungicide wherein is a bacillus subtilis for dilution 100-200 volume ratio doubly: 3 bacterial classifications of actinomycetes: saccharomycete=1:1:1 are formed; Described salt refers to 0.3%-0.9%NaCl saline-alkali soil.
2. the described application of claim 1, lawn plant wherein are perennial ryegrass or Festuca Arundinacea.
3. the described application of claim 1, wherein said salt-resistance refers to: producing fertilizer from refuse in daily life compost microbe microbial inoculum is promoting the perennial ryegrass photosynthetic rate, is alleviating the application aspect the chlorophyll content decline that salt stress causes; Described complex micro organism fungicide is a bacillus subtilis for the volume ratio of 100 times of dilutions: 3 bacterial classifications of actinomycetes: saccharomycete=1:1:1 are formed.
4. the described application of claim 1, wherein said salt-resistance refers to: the application of compost microbe microbial inoculum aspect enhancing root system of plant opposing salt stress; Described salt refers to 0.3%-0.9%NaCl saline-alkali soil.
5. the described application of claim 1, wherein said salt-resistance refers to: the application of compost microbe microbial inoculum aspect increase Festuca Arundinacea chlorophyll content; Complex micro organism fungicide wherein is a bacillus subtilis for the volume ratio of 200 times of dilutions: 3 bacterial classifications of actinomycetes: saccharomycete=1:1:1 are formed.
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| CN102598978A (en) * | 2012-03-02 | 2012-07-25 | 天津师范大学 | Method for improving chromium resistance and cadmium resistance of Festuca arundinacea with compost fermentation liquid |
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