CN105585379A - Biological compound fertilizer and application thereof - Google Patents
Biological compound fertilizer and application thereof Download PDFInfo
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- CN105585379A CN105585379A CN201510958149.5A CN201510958149A CN105585379A CN 105585379 A CN105585379 A CN 105585379A CN 201510958149 A CN201510958149 A CN 201510958149A CN 105585379 A CN105585379 A CN 105585379A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
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- Fertilizers (AREA)
Abstract
The invention discloses a biological compound fertilizer and application thereof. The biological compound fertilizer is prepared from, by weight, 20-30 parts of Bacillus amyloliquefaciens YN2010-KC CGMCC No.5722 fermentation broth, 10-20 parts of organic matter, 2-5 parts of urea, 1-3 parts of calcium superphosphate, 4-6 parts of potassium sulphate, 0.5-2 parts of microelement matter and 5-10 parts of a binding agent. The biological compound fertilizer is small in dosage, becomes effective rapidly, and is long in valid period, and capable of effectively promoting absorption of plants to a phosphorus potassium fertilizer, promoting plant root system development, improving soil, avoiding or reducing plant diseases and insect pests, obviously improving quality and improving the yield by 10-25%.
Description
Technical field
The invention belongs to microorganism fertilizer technical field, be specifically related to adopt answering of microorganism, organic matter and inorganic matterHefei and application.
Background technology
Chemical fertilizer had once been made huge contribution for agricultural production, but present also let us is paid heavy cost. For a long timeUse chemical fertilizer, the destruction of causing soil aggregate, soil compaction, also causes the famine of the soil organism,Fertility declines, the degradation of gas permeability, water resistance, and ecological environment of soil worsens, and causes microorganism species numberAmount reduces, and plant root system development is bad, and disease and pest increases, and finally causes crop yield to decline, under crop qualityFall, affected the mankind's health existence. And biologic compound fertilizer has original effect on addressing these problems.
Biologic compound fertilizer has not only brought convenience to agricultural production, and brings and carry to people's living standardHeight, its appearance has guided the major transformation of an agriculture new round.
Biologic compound fertilizer and conventional fertilizers: chemical fertilizer, fertilizer and microbial manure are compared, taking organic substance asMain body, coordinates a small amount of chemical fertilizer, carries out scientific matching according to the regulation of fertilizer requirement of crops and fertilizer characteristic,Except containing trace unit in nitrogen, phosphorus, potassium macronutrient and calcium, magnesium, sulphur, iron, boron, zinc, selenium, molybdenum etc.Outside element, also contain large amount of organic matter, humic acid material and fertilizer conservation synergist, nutrient is complete, the slow Ji mutually of speed,Fertilizer equilibrium, fertilizer efficiency is lasting. The microbial inoculum that it is contained, can chesson, strengthens soil aggregate, improvesPreserve moisture and fertility ability, increases the soil organism, and the potential nutrients in activating soil improves chemical fertilizer utilization ratio. RawThing composite fertilizer can promote crop rhizosphere beneficial microorganism propagation, improves crop rhizosphere ecological environment. Useful micro-lifeThe increase of thing and disease-resistant factor, also can reduce infecting of soil-borne disease significantly, and the state of an illness that reduces continuous cropping crop refers toNumber, uses year after year and can greatly alleviate continuous cropping obstacle. The alternative chemical fertilizer of biological compound fertilizer carries out single fertilization, fallsLow production cost. Therefore, development biological compound fertilizer is the needs of China's sustainable development agricultural.
Biological compound fertilizer is the elite of drawing traditional organic fertilizer, in conjunction with modern biotechnology, and the height processingSci-tech product. Its nutrient integrates quick-acting, long-acting, synergy, has the quality of agricultural product of raising, inhibitionThe effect of soil-borne disease, enhancing crop anti-adversity, promotion crop precocity.
Summary of the invention
For making up the deficiency of chemical fertilizer and microbial manure unicity, the invention provides one and gathered traditional fertilizerWith the advantage of bacterial manure, compound to beneficial microbe, organic matter and inorganic matter suitable proportion is made a kind of biological compoundFertilizer.
Technical scheme of the present invention is as follows:
1. a biological compound fertilizer, is characterized in that: by weight, be made up of following material:
Bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.572220～30 parts of zymotic fluids, 10～20 parts of organic matters, 2～5 parts, urea, 1～3 part of calcium superphosphate, potassium sulfate 4～6Part, 0.5～2 part of microelement substance, 5～10 parts of binding agents.
2. according to the biological compound fertilizer described in technical scheme 1, it is characterized in that: described bacillus amyloliquefaciens(Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 zymotic fluid is by following methods systemStandby obtaining:
(1) by described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCAfter CGMCCNo.5722 activation, by 5～10% inoculum concentrations, by the bacillus amyloliquefaciens (Bacillus of activationAmyloliquefaciens) YN2010-KCCGMCCNo.5722 is inoculated into seed culture medium, is cultivating temperatureSpend under 35 DEG C, rotating speed 120rpm/min condition, shaking table is cultivated 36～48h, obtains primary seed solution, described inSeed culture medium is liquid LB culture medium, pH7.0～7.2;
(2) by 10～12% inoculum concentrations, primary seed solution is transferred to the secondary seed medium in seeding tank,At 28～37 DEG C, rotating speed 100rpm/min, throughput is 1000L/h, tank pressure remains on 0.02～0.03MPaUnder condition, cultivate 36～48h, obtain secondary seed solution; Described secondary seed medium is by mass fractionBelow become to be grouped into: starch 1.5～3%, wheatfeed 1～2%, yeast extract 0.05～0.3%, peptone 0.5～1%,Ground phosphate rock 1～2%, MgSO4·7H2O0.01～0.05％，CaCl20.1～0.4％，(NH4)2SO40.01～0.04％，Surplus is water, pH7.0～7.2;
(3) by 10～12% inoculum concentrations, secondary seed solution is inoculated into the fermentation medium in fermentation tank,28～37 DEG C, rotating speed 100rpm/min, throughput is that 10000L/h, tank pressure remain on 0.02～0.03MPa barUnder part, cultivate 48～72h, bacillus amyloliquefaciens in the zymotic fluid after cultivation (Bacillusamyloliquefaciens)You effect Jun alive Shuo≤1 × 10 of YN2010-KCCGMCCNo.57228When cfu/ml, this zymotic fluid is instituteStating bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 sends outFerment liquid; Described fermentation medium is identical with the described secondary seed medium of step (2).
3. according to the biological compound fertilizer described in technical scheme 1, it is characterized in that: described organic matter be rape cake,Any one or any two or more in fish meal, the peat composed of rotten mosses, humic acid, organic matter is any when two or more, eachOrganic matter is equivalent.
4. according to the biological compound fertilizer described in technical scheme 1, it is characterized in that: described microelement substance isBoric acid or ferrous sulfate.
5. according to the biological compound fertilizer described in technical scheme 1, it is characterized in that: described binding agent is diatomiteOr kaolin.
6. the application of the biological compound fertilizer described in arbitrary technical scheme on plantation romaine lettuce in technical scheme 1 to 5.
Compared with prior art, the present invention has following beneficial effect:
1, can to replace chemical fertilizer be completely base manure or the use of topdressing to biologic compound fertilizer of the present invention, its toolThe quick-acting of organic/inorganic substance, fertilizer long-lasting, also has microbial bacterial agent synergy.
2, biologic compound fertilizer of the present invention is nutritious, and the content of organic matter is high. This fertilizer subtracts at habits fertilizationUnder the condition of amount 10%, also can increase production 7.78% than habits fertilization, can meet the life of organic food and pollution-free foodProduce and need.
3, biologic compound fertilizer of the present invention is formed by microorganism fermentation, the effective viable bacteria of product up to1×108More than cfu/ml, can improve the micro-ecological environment of soil, for the growth of crops provides beneficial conditions.
4, biologic compound fertilizer of the present invention can reduce infecting of soil-borne disease significantly, reduction continuous cropping cropDisease index, uses year after year and can greatly alleviate continuous cropping obstacle.
5, biologic compound fertilizer of the present invention can strengthen the drought-resistant ability of crop. Microorganism used therefor energy in the present inventionSecretion growth promoting substance, extraneous root strong sprout; Decompose element silicon in soil and, for plant utilization, make the wax coat of plantThicken, improve plant water keeping ability.
6, biologic compound fertilizer raw material of the present invention is easy to get, and technique is simple, and energy consumption is low, can give birth in enormous quantitiesProduce, be not subject to resource limitation.
Bacillus amyloliquefaciens BacillusamyloliquefaciensYN2010-KC of the present invention is in 2012Be deposited on January 12, China Committee for Culture Collection of Microorganisms's common micro-organisms center (be called for short:CGMCC), preserving number is CGMCCNo.5722, and detects as survival on January 12nd, 2012.This common micro-organisms centre address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101.
Detailed description of the invention
Further illustrate below in conjunction with embodiment, each embodiment is conventional method without specified otherwise.
Embodiment 1 bacillus amyloliquefaciens of the present invention (Bacillusamyloliquefaciens) YN2010-KCAcquisition, preservation
1, the acquisition of bacillus amyloliquefaciens YN2010-KC of the present invention
1.1 material
Pick up from the Hou Shan of Yunnan Prov Agriculture University farm Rhizosphere of Crops soil around.
1.2 culture medium preparations
LB solid medium and PSA culture medium (potato sucrose agar medium) are melted, pour into respectivelySterilizing culture dish, cooling, make LB and PSA flat board.
1.3 separate cultivation
Adopt dilution plate partition method to separate with plate streaking, by a bacterial strain called after of separation and purification gainedYN2010-KC。
2, the qualification of bacillus amyloliquefaciens YN2010-KC
2.1, Morphological Identification
Get bacterial strain YN2010-KC and cultivate on LB culture medium, cultivate 36 hours, carry out form sight for 37 DEG CExamine qualification, thalline is shaft-like, end circle, and peritrichous, tool motility, produce oval gemma; At LB andBacterium colony circle on PSA flat board, weak tea brown, bacterium colony central color are slightly dark, protuberance, dry tack free have fold.With description in " common bacteria system identification handbook " (eastern elegant pearl etc. are write, Science Press, calendar year 2001)Bacillus morphological feature basically identical, tentatively judge that bacterial strain YN2010-KC belongs to bacillus(Bacillusspp.)。
2.2, Physiology and biochemistry qualification
Bacterial strain Gram's staining, spore staining, Starch Hydrolysis, glucose utilization, citrate utilization, V-PReaction, catalase reaction, nitrate reduction, gelatin liquefaction etc. are all positive.
3, the preservation of bacillus amyloliquefaciens YN2010-KC
Through above-mentioned identify show separate obtain bacterial strain be bacillus amyloliquefaciens (BacillusAmyloliquefaciens), its code name is YN2010-KC, described bacillus amyloliquefaciens BacillusAmyloliquefaciensYN2010-KC is deposited in Chinese microorganism strain preservation pipe on January 12nd, 2012Reason committee common micro-organisms center (be called for short: CGMCC), preserving number is CGMCCNo.5722, andDetect as survival on January 12nd, 2012. This common micro-organisms centre address: Chaoyang District, city of BeiJing, ChinaNo. 3, No. 1, North Star West Road institute, postcode: 100101.
Embodiment 2 bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCThe preparation of No.5722 zymotic fluid
(1) primary seed solution preparation
By the bacillus amyloliquefaciens of-80 DEG C of preservations (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 is seeded on liquid LB culture medium, cultivates 24h for 35 DEG C, obtains the solution starch bud of activationSpore bacillus (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722, by 10% inoculationAmount, by described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KC of activationCGMCCNo.5722 is inoculated into seed culture medium, in 35 DEG C of cultivation temperature, rotating speed 120rpm/min conditionUnder, shaking table is cultivated 36h, obtains primary seed solution, and described seed culture medium is liquid LB culture medium, pH7.0～7.2；
(2) by 10% inoculum concentration, primary seed solution is transferred to the secondary seed medium in seeding tank,35 DEG C, rotating speed 100rpm/min, throughput is 1000L/h, tank pressure remains under 0.02～0.03MPa conditionCultivate 36～48h, obtain secondary seed solution; Described secondary seed medium is by the following one-tenth in mass fractionBe grouped into: starch 2%, wheatfeed 1%, yeast extract 0.3%, peptone 0.5%, ground phosphate rock 1%, MgSO4·7H2O0.02％，CaCl20.25％，(NH4)2SO40.03%, surplus is water, pH7.0～7.2, described secondary seedCultivate based on 121 DEG C of sterilizing 30min, wait to be cooled to 30 DEG C, access primary seed solution.
(3) by 10% inoculum concentration, secondary seed solution is inoculated into the fermentation medium in fermentation tank, at 35 DEG C,Rotating speed 100rpm/min, throughput is that 10000L/h, tank pressure remain under 0.02～0.03MPa condition and cultivate48～72h, bacillus amyloliquefaciens in the zymotic fluid after cultivation (Bacillusamyloliquefaciens)The living bacteria count of YN2010-KCCGMCCNo.5722 is 1 × 108When cfu/ml, this zymotic fluid is instituteStating bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 sends outFerment liquid, described fermentation medium is identical with the described secondary seed medium of step (2).
Embodiment 3 biological compound fertilizer formula examples of the present invention
Table 11 biological compound fertilizer formula:
Table 22 biological compound fertilizer formula:
The preparation of embodiment 4 biological compound fertilizers of the present invention
In embodiment 3, the preparation of No. 1 biological compound fertilizer and No. 2 biological compound fertilizers is all as follows:
Take by formula metering the 4-5 days that banks up after each component is mixed, then granulation, 35 DEG C of oven dry, to obtain final productObtain biological compound fertilizer.
No. 1 biological compound fertilizer field test on romaine lettuce in embodiment 5 embodiment 3
No. 1 biological compound fertilizer is called for short: No. 1 product. Experiment is entered in Yue Zhou town, Qilin District, Qujing peasant household farmland covered by contractOK, the soil texture is clay, and soil fertility is medium; Be Italian romaine lettuce kind 333 for studying thing. Employing is adoptedArrange experimental design by random district group, establish 3 processing, repeat community area 20.0m for 3 times2. Every community kindPlant 105 strains, mu strain number is 3500 strains. Test is treated to:
Process 1: for mu is executed product+habits fertilization decrement 10% No. 1: mu execute No. 1 product 100kg, farm manure 900kg,It is disposable employed that composite fertilizer 4.5kg makes base fertilizer, and mu is executed urea 45kg and topdressed, wherein 10% do to topdress for the 1st time,30% does to topdress for the 2nd time, and 60% does to topdress for the 3rd time.
Process 2: be habits fertilization: it is disposable employed that mu is executed farm manure 1000kg, composite fertilizer 5kg makes base fertilizer, mu is executed urineElement 50kg topdresses, and wherein 10% does to topdress for the 1st time, and 30% does to topdress for the 2nd time, and 60% does to topdress for the 3rd time. DescribedN:P in composite fertilizer2O5：K2The mass ratio of O is 5:10:5.
Process 3: be blank (CK): do not execute any fertilizer.
The impact of table 1 different disposal on romaine lettuce output
The variance analysis of table 2 output and F test
Gather in the crops result from table 1, No. 1 product, under the condition of habits fertilization decrement 10%, is used on romaine lettuceNo. 1 product also has good effect of increasing production, uses No. 1 product and increases production and reach 14.76% compared with the control, executes with customFertilizer is compared volume increase 7.78%, and difference reaches the utmost point level of signifiance.
Claims (6)
1. a biological compound fertilizer, is characterized in that: by weight, be made up of following material:
Bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.572220～30 parts of zymotic fluids, 10～20 parts of organic matters, 2～5 parts, urea, 1～3 part of calcium superphosphate, potassium sulfate4～6 parts, 0.5～2 part of microelement substance, 5～10 parts of binding agents.
2. biological compound fertilizer according to claim 2, is characterized in that: described bacillus amyloliquefaciens(Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 zymotic fluid is by following methods systemStandby obtaining:
(1) by described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCAfter CGMCCNo.5722 activation, by 5～10% inoculum concentrations, by the bacillus amyloliquefaciens (Bacillus of activationAmyloliquefaciens) YN2010-KCCGMCCNo.5722 is inoculated into seed culture medium, is cultivating temperatureSpend under 35 DEG C, rotating speed 120rpm/min condition, shaking table is cultivated 36～48h, obtains primary seed solution, described inSeed culture medium is liquid LB culture medium, pH7.0～7.2;
(2) by 10～12% inoculum concentrations, primary seed solution is transferred to the secondary seed medium in seeding tank,At 28～37 DEG C, rotating speed 100rpm/min, throughput is 1000L/h, tank pressure remains on 0.02～0.03MPaUnder condition, cultivate 36～48h, obtain secondary seed solution; Described secondary seed medium is by mass fractionBelow become to be grouped into: starch 1.5～3%, wheatfeed 1～2%, yeast extract 0.05～0.3%, peptone 0.5～1%,Ground phosphate rock 1～2%, MgSO4·7H2O0.01～0.05％，CaCl20.1～0.4％，(NH4)2SO40.01～0.04％，Surplus is water, pH7.0～7.2;
(3) by 10～12% inoculum concentrations, secondary seed solution is inoculated into the fermentation medium in fermentation tank,28～37 DEG C, rotating speed 100rpm/min, throughput is that 10000L/h, tank pressure remain on 0.02～0.03MPa barUnder part, cultivate 48～72h, bacillus amyloliquefaciens in the zymotic fluid after cultivation (Bacillusamyloliquefaciens)You effect Jun alive Shuo≤1 × 10 of YN2010-KCCGMCCNo.57228When cfu/ml, this zymotic fluid is instituteStating bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 sends outFerment liquid; Described fermentation medium is identical with the described secondary seed medium of step (2).
3. biological compound fertilizer according to claim 1, is characterized in that: described organic matter be rape cake,Any one or any two or more in fish meal, the peat composed of rotten mosses, humic acid, organic matter is any when two or more, eachOrganic matter is equivalent.
4. biological compound fertilizer according to claim 1, is characterized in that: described microelement substance isBoric acid or ferrous sulfate.
5. biological compound fertilizer according to claim 1, is characterized in that: described binding agent is diatomiteOr kaolin.
6. the application of the biological compound fertilizer described in arbitrary claim on plantation romaine lettuce in claim 1 to 5.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107721713A (en) * | 2016-08-12 | 2018-02-23 | 南京轩凯生物科技有限公司 | A kind of microbial compound bacterial fertilizer and preparation method and application |
| CN108377819A (en) * | 2018-02-05 | 2018-08-10 | 云南省烟草公司普洱市公司 | A method of prevention tobacco black shank |
| CN111286472A (en) * | 2019-12-20 | 2020-06-16 | 云南省微生物发酵工程研究中心有限公司 | Bacillus amyloliquefaciens with fragrance production function and preparation method and application thereof |
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| CN104911129A (en) * | 2015-06-10 | 2015-09-16 | 湖南泰谷生物科技股份有限公司 | Bacillus amyloliquefaciens strain for prevention and treatment of tomato bacterial wilt and microbial organic fertilizer thereof |
| CN105036918A (en) * | 2015-07-20 | 2015-11-11 | 湖南泰谷生物科技股份有限公司 | Multielement biologic organic fertilizer and preparing method thereof |
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| CN102864100A (en) * | 2012-08-16 | 2013-01-09 | 北京雷力农用化学有限公司 | Bacillus amyloliquefaciens and application thereof |
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| CN107721713A (en) * | 2016-08-12 | 2018-02-23 | 南京轩凯生物科技有限公司 | A kind of microbial compound bacterial fertilizer and preparation method and application |
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| CN111286472A (en) * | 2019-12-20 | 2020-06-16 | 云南省微生物发酵工程研究中心有限公司 | Bacillus amyloliquefaciens with fragrance production function and preparation method and application thereof |
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Application publication date: 20160518 |