CN102138443B - Propagation method for increasing grafting survival rate of Madhuca hainanensis Chun et How - Google Patents
Propagation method for increasing grafting survival rate of Madhuca hainanensis Chun et How Download PDFInfo
- Publication number
- CN102138443B CN102138443B CN2011100765832A CN201110076583A CN102138443B CN 102138443 B CN102138443 B CN 102138443B CN 2011100765832 A CN2011100765832 A CN 2011100765832A CN 201110076583 A CN201110076583 A CN 201110076583A CN 102138443 B CN102138443 B CN 102138443B
- Authority
- CN
- China
- Prior art keywords
- scion
- stock
- grafting
- capital
- sub
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Materials For Medical Uses (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a propagation method for increasing the grafting survival rate of Madhuca hainanensis Chun et How. The method comprises the following steps of: cutting half-lignified or lignified sun-exposed erect branches serving as scions from a Madhuca hainanensis Chun et How parent tree, removing leaves, keeping 2-4 bud eyes on each section of scion, scattering the scions, and standing for 2-3 days until myron natural juice flows out; stumping parts, which are 10 centimeters away from base parts, of 1-2 year old Madhuca hainanensis Chun et How seedlings serving as root stocks and standing for 2-3 days to make myron juice flows out naturally until incision juice is solidified; and grafting by adopting scion-root stock double side whip grafting. The Madhuca hainanensis Chun et How scions and the root stocks are pretreated, so that the problems of difficulty in operating and easily caused joint infection of a grafted plant due to excessive juice of Madhuca hainanensis Chun et How are solved; the grafting method is improved and the scion-root stock double side whip grafting is used for grafting; and after the method disclosed by the invention is used for grafting, a wound starts to heal 8-12 days later, new buds can grow from the scions 20-25 days later, and the survival rate is increased by 22 percent and is up to 96 percent.
Description
Technical field
The present invention relates to the propagation method of a kind of arbor, be specifically related to a kind of propagation method that improves sub-capital graft survival rate.
Background technology
(Madhuca hainanensis Chun et How) belongs to evergreen high megaphanerophyte for Sapotaceae (Sapotaceae) Madhuca pasquieri in sub-capital; The height of tree can reach 25～30m; Diameter of a cross-section of a tree trunk 1.3 meters above the ground 80cm calls and is madhuca hainanensis, and special product is in China Hainan Province; Be Hainan Wu Date class commodity with one of material, have the title of " green steel plate ".Sub-capital wood quality is thick and heavy and tough and tensile, and compact structure is and even, and tangent plane is smoothly glossy, and is attractive in appearance, and utmost point corrosion resistant is specially adapted to make sleeper, mechanical implement, shipbuilding, bridge etc., and it is splendid to be used for aspects such as vehicle, building, furniture.Sub-capital bark tool medical value can be clearing heat and detoxicating; Its trunk is perfectly straight, the tree crown expansion, and branches and leaves are dark green, and growing situation is tall and graceful, is the fine tree species of afforestation.In addition, sub-capital seed oil content 50～55% can extract oil, and oily edible or soap are industrial.Bark contains tannin 19.11%, can obtain through refining tannin extract.
The natural southwestern mountain area that only is distributed on the south the middle part, China Hainan, sub-capital, scope is very narrow.Human in order to open up living space such as grainfield; The reclamation of wasteland of centuries and middle and later periods in last century are to the predatory felling of Hainan natural forest resource; Make to comprise that many natural forest in sub-capital are unforosted, breakdown strength is big, forest ruined or fragmentation serious; The existence of sub-capital is on the hazard, and existing natural resources sharply reduce.
At present, sub-capital has been classified China's secondary endangered as and has been laid special stress on protecting plant, and its basic research is weak.The foundation of the effective propagation technique system in sub-capital is that it is carried out the artificial prerequisite of preserving and developing.
In the sub-capital propagation by grafiting procedure of exploitation, find that sub-capital tree body contains abundant white juice, juice outflow and many rapidly during the grafting cutting; Adopt traditional direct grafting of whip grafting; The myron liquid of scion of antithetical phrase capital and stock outflow does not carry out the processing in early stage, operating difficulties, and cause wound infection easily; Be unfavorable for the healing of interface, the survival rate of grafting is merely 74%.
Summary of the invention
In order to solve the deficiency of prior art; The object of the present invention is to provide a kind of propagation method that improves sub-capital graft survival rate; Proper process is carried out in this method antithetical phrase capital graft stock and scion, has got rid of the influence of a large amount of white juice in the tree body of sub-capital, makes more convenient to operate; Adopt stock, the two-sided whip grafting of scion, make grafting plant survival rate higher.
The object of the invention is realized through following technical proposals:
A kind of propagation method that improves sub-capital graft survival rate may further comprise the steps:
(1) collection of scion and processing
Clip semi-lignified or the lignified vertical branch that faces south from the select tree of natural forest lining capital are cut into the long scion of 6～10cm, and every section scion keeps 2～4 eyes, removes blade, and the upper end of scion is cut to flat nick; Under the condition of 24～28 ℃ of degree of shading 80～95%, humidity 70～95% and temperature; Scion is spread out on clean preservative film, cover with preservative film more above and preserve moisture, placed 2～3 days; Let myron liquid flow out naturally; Solidify up to otch juice, like this during grafting again cut wound mouth juice seldom help the grafting operation and improve survival rate;
(2) preparation of stock and processing
1～2 year sub-capital of life seedling with the sub-capital seminal propagation of gathering is done stock; Cut driedly from the about 10cm eminence of base portion, under the condition of 24～28 ℃ of degree of shading 80～95%, humidity 70～95% and temperature, placed 2～3 days; Solidify up to otch juice, let myron liquid flow out naturally;
(3) grafting operation
Grafting is carried out under the environment of 24～28 ℃ of degree of shading 80～95%, humidity 70～95% and temperature, selects more consistent step (2) stock and step (1) scion of thickness; Have a little the xylem rip cutting in stock one side and remove about 2～2.5cm, the about 3cm of stock opposite side longitudinal incision makes stock top wedgewise (shown in Figure 1A); Have a little the xylem rip cutting in scion one side and remove 2～2.5cm, the about 3cm of scion opposite side longitudinal incision makes scion bottom wedgewise (shown in Figure 1B); It is level and smooth that all tangent planes are all wanted; Then (shown in Fig. 1 C) inserted in scion and the long tangent plane of stock 3cm in correspondence with each other; Make cambium separately be close to alignment in twos, thumb and forefinger are clutched the interface of stock and scion then, tightly wrap the fastening wrapping of interface with bandage; And whole scion twined one deck bandage, prevent the scion water evaporates and dry up.
(4) later stage is managed and protected
Will keep matrix moistening after the grafting, trickle was noted drenching grafting mouth and scion in preceding 10 days, and anti-sealing infiltrates and causes infection of incisional wound to rot.
The preferred operating environment of said step (1), (2), (3) is: 25 ℃ of degree of shading 90%, humidity 80%, temperature.
The grow directly from seeds leading thread of seedling of the said sub-capital of step (2) is 0.5～1.5cm.
The said matrix of step (4) is Hainan laterite.
The time optimal of said grafting operation selects annual sub-capital to take out 3～April of sprouting for the first time, the September of secondly taking out sprouting for the second time for annual sub-capital; But also grafting in other season, but the time that sprouts is wanted slow and survival rate is low.Grafting in 3～April, rear port began healing in 8～12 days, but 20～25 days scion eruption sproutings, sprouting can be worn out bandage, and can be decided to be grafting survival this moment.Other month grafting wound healing wants a couple of days in evening to two weeks, and scion grows the time of sprouting and then wants late two weeks to 1 first quarter moon.
Principle of the present invention is to utilize the injured back of plant self to produce callus through the hyperplasia of wound; Two cambium of hindering face are fitted and tightened together, can heal and link together into as a whole through vascular tissue because of hyperplasia each other.
The present invention has following advantage and beneficial effect with respect to prior art:
(1) early stage processing and the correlation techniques such as store method, grafting time, grafting method and final-period management of the present invention through groping scion of sub-capital and stock; Rapid outflow of juice and amount are many when having solved the grafting cutting of sub-capital; Influence the grafting operation; And cause wound infection easily, be unfavorable for the problems such as healing of interface.
(2) the present invention has improved grafting method; On the basis of traditional grafting method, the role has been exchanged in scion and stock 1 time; Thereby twice of grafting simultaneously; Two-sided whip grafting makes scion and stock respectively have 2 cambium to fit each other, compares with conventional method, and the sub-capital grafting method survival rate after the improvement brings up to 96% from 74%.
(3) after the grafting of employing the inventive method, nutrient and water consumption are few, and interface healing in sub-capital is fast, and it is short to sprout the sprouting required time, the graft survival rate height.Wound begins healing after 8～12 days, but 20～25 days scion eruption sproutings.
(4) the present invention provides a cover new asexual reproduction method for the sub-capital of rare endangered species; This method can make the seedling of propagation by grafiting keep the good hereditary capacity of original elite stand; Thereby artificially collecting with the strange land preservation of the excellent strain of sub-capital natural resources is achieved; Also for its foundation of cultivating good plant and grafting clonal seed orchard provides technical support, application prospect is wide.
Description of drawings
Fig. 1 is the two-sided whip grafting sketch map in sub-capital; Wherein, the A-stock, the B-scion, the C-stock engages sketch map with scion.
Embodiment
Below in conjunction with embodiment and accompanying drawing the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
Sub-capital grafting operating procedure (in 3～April, Hainan heat and drought condition carries out season):
(1) collection of scion and processing
Clip semi-lignified or the lignified vertical branch that faces south from the elite stand of sub-capital are cut into the long scion of 8cm, remove blade, and the upper end of scion is cut to flat nick, and every section scion keeps 3 eyes; Under the condition of 25 ℃ of degree of shading 90%, humidity 80% and temperature, in the greenhouse scion is spread out on clean preservative film, cover with preservative film more above and preserve moisture, placed 2～3 days, solidify up to otch juice, let myron liquid flow out naturally;
(2) preparation of stock and processing
Do stock with 1～2 year sub-capital of life seedling that grows directly from seeds, cut driedly from the about 10cm eminence of base portion, under the condition of 25 ℃ of degree of shading 90%, humidity 80% and temperature, placement is 2～3 days in the greenhouse, solidifies up to otch juice, lets myron liquid flow out naturally;
(3) grafting operation
Grafting should be carried out in the greenhouse, 25 ℃ of degree of shading 90%, humidity 80%, temperature.Concrete operation method is to select more consistent stock and the scion of thickness; Have a little the xylem rip cutting in stock one side and remove about 2～2.5cm, the about 3cm of stock opposite side longitudinal incision makes stock top wedgewise (shown in Figure 1A); Have a little the xylem rip cutting in scion one side and remove 2～2.5cm, the about 3cm of scion opposite side longitudinal incision makes scion bottom wedgewise (shown in Figure 1B); It is level and smooth that all tangent planes are all wanted; Then (shown in Fig. 1 C) inserted in scion and the long tangent plane of stock 3cm in correspondence with each other; Make cambium separately be close to alignment in twos, thumb and forefinger are clutched the interface of stock and scion then, tightly wrap the fastening wrapping of interface with bandage; And whole scion twined one deck bandage, prevent the scion water evaporates and dry up.
(4) final-period management
Keep matrix (laterite) moistening after the grafting, trickle was noted drenching grafting mouth and scion in preceding 10 days, and anti-sealing infiltrates and causes infection of incisional wound to rot.
Growing state after the grafting:
Grafting in 3～April, wound begins healing after 8～12 days, but 20～25 days scion eruption sproutings, sprouting can be worn out bandage, and can be decided to be grafting survival this moment, and graft survival rate is up to 96%.
Embodiment 2
Sub-capital grafting operating procedure (in September, Hainan high-temperature high humidity season carries out):
(1) collection of scion and processing
Clip semi-lignified or the lignified vertical branch that faces south from the elite stand of sub-capital are cut into the long scion of 10cm, remove blade, and the upper end of scion is cut to flat nick, and every section scion keeps 4 eyes; Under the condition of 28 ℃ of degree of shading 80%, humidity 95% and temperature, in the greenhouse scion is spread out on clean preservative film, cover with preservative film more above and preserve moisture, placed 2～3 days, solidify up to otch juice, let the myron flow go out;
(2) preparation of stock and processing
Do stock with 1～2 year sub-capital of life seedling that grows directly from seeds, cut driedly from the about 10cm eminence of base portion, under the condition of 28 ℃ of degree of shading 80%, humidity 95% and temperature, placement is 2～3 days in the greenhouse, solidifies up to otch juice, lets myron liquid flow out naturally;
(3) grafting operation
Grafting should be carried out in the greenhouse, 28 ℃ of degree of shading 80%, humidity 95%, temperature.Concrete operation method is to select more consistent stock and the scion of thickness; Have a little the xylem rip cutting in stock one side and remove about 2～2.5cm, the about 3cm of stock opposite side longitudinal incision makes stock top wedgewise (shown in Figure 1A); Have a little the xylem rip cutting in scion one side and remove 2～2.5cm, the about 3cm of scion opposite side longitudinal incision makes scion bottom wedgewise (shown in Figure 1B); It is level and smooth that all tangent planes are all wanted; Then (shown in Fig. 1 C) inserted in scion and the long tangent plane of stock 3cm in correspondence with each other; Make cambium separately be close to alignment in twos, thumb and forefinger are clutched the interface of stock and scion then, tightly wrap the fastening wrapping of interface with bandage; And whole scion twined one deck bandage, prevent the scion water evaporates and dry up.
(4) final-period management
Will keep matrix (laterite) moistening after the grafting, trickle was noted drenching grafting mouth and scion in preceding 10 days, and anti-sealing infiltrates and causes infection of incisional wound to rot.
After the grafting after 10～12 days wound begin the healing, but 25～30 days scion eruption sproutings, sprouting can be worn out bandage, this moment can be decided to be grafting survival.Graft survival rate is up to 96%.
Embodiment 3
Sub-capital grafting operating procedure (in December～January next year, Hainan arid low temperature season carries out):
(1) collection of scion and processing
Clip semi-lignified or the lignified vertical branch that faces south from the elite stand of sub-capital are cut into the long scion of 6cm, remove blade, and the upper end of scion is cut to flat nick, and every section scion keeps 2 eyes; Under the condition of 24 ℃ of degree of shading 95%, humidity 70% and temperature, in the greenhouse scion is spread out on clean preservative film, cover with preservative film more above and preserve moisture, placed 2～3 days, solidify up to otch juice, let myron liquid flow out naturally;
(2) preparation of stock and processing
Do stock with 1～2 year sub-capital of life seedling that grows directly from seeds, cut driedly from the about 10cm eminence of base portion, under the condition of 24 ℃ of degree of shading 95%, humidity 70% and temperature, placement is 2～3 days in the greenhouse, solidifies up to otch juice, lets myron liquid flow out naturally;
(3) grafting operation
Grafting should be carried out in the greenhouse, 24 ℃ of degree of shading 95%, humidity 70%, temperature.Concrete operation method is to select more consistent stock and the scion of thickness; Have a little the xylem rip cutting in stock one side and remove about 2～2.5cm, the about 3cm of stock opposite side longitudinal incision makes stock top wedgewise (shown in Figure 1A); Have a little the xylem rip cutting in scion one side and remove 2～2.5cm, the about 3cm of scion opposite side longitudinal incision makes scion bottom wedgewise (shown in Figure 1B); It is level and smooth that all tangent planes are all wanted; Then (shown in Fig. 1 C) inserted in scion and the long tangent plane of stock 3cm in correspondence with each other; Make cambium separately be close to alignment in twos, thumb and forefinger are clutched the interface of stock and scion then, tightly wrap the fastening wrapping of interface with bandage; And whole scion twined one deck bandage, prevent the scion water evaporates and dry up.
(4) final-period management
Keep matrix (laterite) moistening after the grafting, trickle was noted drenching grafting mouth and scion in preceding 15 days, and anti-sealing infiltrates and causes infection of incisional wound to rot.
After the grafting after 15～20 days wound begin the healing, but 60～70 days scion eruption sproutings, sprouting can be worn out bandage, this moment can be decided to be grafting survival.Graft survival rate is up to 90%.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (3)
1. propagation method that improves sub-capital graft survival rate is characterized in that may further comprise the steps:
(1) clip semi-lignified or the lignified vertical branch that faces south from the elite stand of sub-capital are cut into the long scion of 6～10cm, and every section scion keeps 2～4 eyes, removes blade; Scion was spread out, placed 2～3 days, solidify, let myron liquid flow out naturally up to otch juice;
(2) do stock with 1～2 year sub-capital of life seedling that grows directly from seeds, cutting driedly from base portion 10cm eminence, then stock was placed 2～3 days, solidify, let myron liquid flow out naturally up to otch juice;
(3) select thickness consistent stock and scion; The place that has a little xylem in stock one side vertically cuts 2～2.5cm, and the stock opposite side vertically cuts 3cm, makes stock top wedgewise; The place that has a little xylem in scion one side vertically cuts 2～2.5cm, and the scion opposite side vertically cuts 3cm, makes scion bottom wedgewise; Then scion and the long tangent plane of stock 3cm are inserted in correspondence with each other, make cambium separately be close to alignment in twos, clutch the interface of stock and scion, tightly wrap the fastening wrapping of interface with bandage, and whole scion is twined one deck bandage;
The environment of the placement environment of said scion, stock and step (3) grafting operation is, 24～28 ℃ of degree of shading 80～95%, humidity 70～95%, temperature.
2. according to the said a kind of propagation method that improves sub-capital graft survival rate of claim 1, it is characterized in that: the environment of the placement environment of said scion, stock and step (3) grafting operation is, 25 ℃ of degree of shading 90%, humidity 80%, temperature.
3. according to the said a kind of propagation method that improves sub-capital graft survival rate of claim 1, it is characterized in that: the grow directly from seeds leading thread of seedling of the said sub-capital of step (2) is 0.5～1.5cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100765832A CN102138443B (en) | 2011-03-29 | 2011-03-29 | Propagation method for increasing grafting survival rate of Madhuca hainanensis Chun et How |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100765832A CN102138443B (en) | 2011-03-29 | 2011-03-29 | Propagation method for increasing grafting survival rate of Madhuca hainanensis Chun et How |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102138443A CN102138443A (en) | 2011-08-03 |
| CN102138443B true CN102138443B (en) | 2012-05-30 |
Family
ID=44406425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100765832A Expired - Fee Related CN102138443B (en) | 2011-03-29 | 2011-03-29 | Propagation method for increasing grafting survival rate of Madhuca hainanensis Chun et How |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102138443B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919062A (en) * | 2012-11-29 | 2013-02-13 | 宁都宇林生物科技开发有限公司 | Wild varnish tree grafting planting method |
| CN103098653B (en) * | 2013-02-06 | 2014-04-23 | 广东省林业科学研究院 | Grafting method of albizia falcataria |
| CN104303851B (en) * | 2014-09-28 | 2017-01-25 | 定远县金胜农业开发有限公司 | Tomato grafting method capable of improving survival rate |
| CN107223517A (en) * | 2017-06-29 | 2017-10-03 | 成都苗夫现代苗木科技有限公司 | A kind of propagation by grafiting method of Canadian red autumnal leaves cercis |
| CN114223451B (en) * | 2021-11-24 | 2022-11-11 | 福建省春天生态科技股份有限公司 | Method for cultivating cut-stem seedlings |
-
2011
- 2011-03-29 CN CN2011100765832A patent/CN102138443B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102138443A (en) | 2011-08-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103416229B (en) | Wrapping-type walnut bark whip grafting method | |
| CN106472226B (en) | A kind of method of the long root of branch trunk punching Rapid Rooting | |
| CN101911899B (en) | Method for grafting various trees onto fingered citron stocks | |
| CN106962028B (en) | Camellia cuttage grafting integrated rapid propagation method | |
| CN103960063A (en) | Fruit tree grafting method | |
| KR101531793B1 (en) | Method for early cuttings of Populus seedling propagation methods | |
| CN105230353A (en) | Method for grafting and shaping tree bodies of perennial seedling walnut trees | |
| CN102138443B (en) | Propagation method for increasing grafting survival rate of Madhuca hainanensis Chun et How | |
| CN101461311A (en) | Method for breeding poplar with colorful leaves | |
| CN104303928A (en) | Maintenance method for adult tea-oil tree forest | |
| CN104871837A (en) | Twig grafting method for engrafting in crown of camellia oleifera tree | |
| CN102726218A (en) | Grafting technology of Chinese torreya | |
| CN102972214A (en) | Grafting method capable of promoting asexual reproduction of populus euphratica | |
| CN104488502A (en) | Acer palmatum biondia heriyi twig cuttage propagation method | |
| CN104365385A (en) | Tea-oil tree bark-tearing scarf joint method | |
| CN104719000A (en) | Novel osmanthus tree grafting method | |
| CN102405774A (en) | Walnut rectangular bud grafting method | |
| CN106171799A (en) | A kind of Fructus Mali pumilae quaternity cultural method | |
| CN103650930A (en) | Fruit tree grafting method | |
| CN102217491A (en) | Cutting sidegrafting method for single buds of fruit trees | |
| CN103518545A (en) | Method for replacing rootstock heads of high branches of walnut trees | |
| CN105104093A (en) | Reproduction method for eugenia uniflora grafting seedling | |
| CN105123282A (en) | Idesia grafting method | |
| CN109832024A (en) | A kind of sapling engrafting method | |
| CN104126425B (en) | The processing method that a kind of tea tree grafting scion is fresh-keeping |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20140329 |