CN102127581B - 一种提高赤霉素提取收率的方法 - Google Patents
一种提高赤霉素提取收率的方法 Download PDFInfo
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- CN102127581B CN102127581B CN201010599389.8A CN201010599389A CN102127581B CN 102127581 B CN102127581 B CN 102127581B CN 201010599389 A CN201010599389 A CN 201010599389A CN 102127581 B CN102127581 B CN 102127581B
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- filtrate
- gibberellin
- nanofiltration
- papain
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- 229930191978 Gibberellin Natural products 0.000 title claims abstract description 43
- 239000003448 gibberellin Substances 0.000 title claims abstract description 43
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000000605 extraction Methods 0.000 title claims abstract description 9
- 238000001728 nano-filtration Methods 0.000 claims abstract description 37
- 239000000706 filtrate Substances 0.000 claims abstract description 25
- 235000019834 papain Nutrition 0.000 claims abstract description 21
- 108090000526 Papain Proteins 0.000 claims abstract description 17
- 239000004365 Protease Substances 0.000 claims abstract description 17
- 229940055729 papain Drugs 0.000 claims abstract description 17
- 239000000047 product Substances 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 13
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 8
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 8
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 25
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 19
- 239000003375 plant hormone Substances 0.000 claims description 19
- 241000282326 Felis catus Species 0.000 claims description 10
- 238000002425 crystallisation Methods 0.000 abstract description 7
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- 238000000926 separation method Methods 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
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- 230000009286 beneficial effect Effects 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- 238000005189 flocculation Methods 0.000 description 3
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- 239000012528 membrane Substances 0.000 description 3
- 238000002203 pretreatment Methods 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- ASZCVNVMQXNJGH-ZYRRHWMLSA-N (1S,2R,9S,12S)-4,12-dimethyl-13-oxotetracyclo[10.2.1.01,9.03,8]pentadeca-3,5,7-triene-2-carboxylic acid Chemical compound Cc1cccc2[C@H]3CC[C@@]4(C)C[C@@]3(CC4=O)[C@@H](C(O)=O)c12 ASZCVNVMQXNJGH-ZYRRHWMLSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 2
- ASZCVNVMQXNJGH-UHFFFAOYSA-N Gibberic acid Natural products C12CCC(C3)(C)C(=O)CC23C(C(O)=O)C2=C1C=CC=C2C ASZCVNVMQXNJGH-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
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- -1 diterpenoids compound Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
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- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- MIDXCONKKJTLDX-UHFFFAOYSA-N 3,5-dimethylcyclopentane-1,2-dione Chemical compound CC1CC(C)C(=O)C1=O MIDXCONKKJTLDX-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010059378 Endopeptidases Proteins 0.000 description 1
- 102000005593 Endopeptidases Human genes 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- HXQNNWUGOJBGLH-UHFFFAOYSA-N [Br+].[Cl+].C(CCCCCCCCCCC)[N+](C)(C)C Chemical compound [Br+].[Cl+].C(CCCCCCCCCCC)[N+](C)(C)C HXQNNWUGOJBGLH-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000001420 bacteriolytic effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 235000013736 caramel Nutrition 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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CN201010599389.8A CN102127581B (zh) | 2010-12-22 | 2010-12-22 | 一种提高赤霉素提取收率的方法 |
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CN102127581A CN102127581A (zh) | 2011-07-20 |
CN102127581B true CN102127581B (zh) | 2014-11-05 |
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CN109796425B (zh) * | 2019-01-24 | 2022-08-05 | 江西新瑞丰生化股份有限公司 | 一种提高赤霉素ga4和ga7板框收率的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1063309A (zh) * | 1991-01-11 | 1992-08-05 | 北京清华大学 | 赤霉素发酵滤液的溶剂萃取法浓缩工艺 |
CN101531646A (zh) * | 2008-03-13 | 2009-09-16 | 迈德金生物技术(北京)有限公司 | 盐析法提取赤霉素 |
-
2010
- 2010-12-22 CN CN201010599389.8A patent/CN102127581B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1063309A (zh) * | 1991-01-11 | 1992-08-05 | 北京清华大学 | 赤霉素发酵滤液的溶剂萃取法浓缩工艺 |
CN101531646A (zh) * | 2008-03-13 | 2009-09-16 | 迈德金生物技术(北京)有限公司 | 盐析法提取赤霉素 |
Non-Patent Citations (7)
Title |
---|
农用抗生素分离纯化的研究进展;商瑞 等;《广东农业科学》;20081231(第2期);124-127 * |
商瑞 等.农用抗生素分离纯化的研究进展.《广东农业科学》.2008,(第2期),124-127. * |
李洲.乳化的防止和破乳.《液-液萃取在制药工业中的应用》.北京:中国医药科技出版社,2005,153-157. * |
破乳剂"Lp-1231"代PPB在青霉素提炼中的应用;罗远辉 等;《抗生素》;19811231;第6卷(第6期);6-13 * |
罗远辉 等.破乳剂"Lp-1231"代PPB在青霉素提炼中的应用.《抗生素》.1981,第6卷(第6期),6-13. * |
赤霉素固液生产与应用;车明峰 等;《民营科技》;20081231(第5期);12,48 * |
车明峰 等.赤霉素固液生产与应用.《民营科技》.2008,(第5期),12,48. * |
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Owner name: JIANGXI NEW REYPHON BIOCHEMICAL CO. LTD. Free format text: FORMER OWNER: JIANGXI NUCLEAR INDUSTRY RUIFENG CHEMICAL CO., LTD. Effective date: 20140926 |
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C41 | Transfer of patent application or patent right or utility model | ||
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Inventor after: Chen Xiaoming Inventor after: Liu Yixiong Inventor after: Deng Jingkai Inventor before: Chen Xiaoming |
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Free format text: CORRECT: INVENTOR; FROM: CHEN XIAOMING TO: CHEN XIAOMING LIU YIXIONG DENG JINGKAI |
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Effective date of registration: 20140926 Address after: Chengnan Industrial Zone 331307 Ji'an city of Jiangxi province Xingan County (Hejiashan No. 1) Applicant after: Jiangxi Xinruifeng Biochemical Co., Ltd. Address before: Ji'an City Chengnan Industrial Zone 331307 Jiangxi Xingan Applicant before: Jiangxi Nuclear Ruifeng Biochemical Co., Ltd. |
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Address after: Chengnan Industrial Zone 331307 Ji'an city of Jiangxi province Xingan County (Hejiashan No. 1) Patentee after: Jiangxi new Ruifeng biochemical Limited by Share Ltd Address before: Chengnan Industrial Zone 331307 Ji'an city of Jiangxi province Xingan County (Hejiashan No. 1) Patentee before: Jiangxi Xinruifeng Biochemical Co., Ltd. |
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