CN102119993A - Preparation method of onion extract, application of onion extract for prolonging life of Caenorhabditis elegans and food containing onion extract - Google Patents
Preparation method of onion extract, application of onion extract for prolonging life of Caenorhabditis elegans and food containing onion extract Download PDFInfo
- Publication number
- CN102119993A CN102119993A CN2011100533411A CN201110053341A CN102119993A CN 102119993 A CN102119993 A CN 102119993A CN 2011100533411 A CN2011100533411 A CN 2011100533411A CN 201110053341 A CN201110053341 A CN 201110053341A CN 102119993 A CN102119993 A CN 102119993A
- Authority
- CN
- China
- Prior art keywords
- bulbus allii
- allii cepae
- extract
- preparation
- onion extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a preparation method of an onion extract and an application of the onion extract for prolonging the life of Caenorhabditis elegans. The preparation method comprises the following steps of: preparing an extract; and carrying out extraction, purification and concentration for many times to obtain the onion extract. The onion extract prepared by the invention has an obvious effect on prolonging the life of Caenorhabditis elegans. The experiment shows that the onion extract of 30mu g/mL, which contains more than 80% of quercetin, can obviously prolong the life and ultimate life of Caenorhabditis elegans, has an action effect which is superior to that of vitamin E, and can obviously improve the expression quantity of SOD (superoxide dismutase) and CAT (catalase) of Caenorhabditis elegans and improve the survival rate of heat shock experiment.
Description
Technical field
The invention belongs to food, medical technical field, especially a kind of preparation method of Bulbus Allii Cepae extract reaches and is prolonging the application of nematicide in the life-span and the food that comprises the Bulbus Allii Cepae extraction.
Background technology
Bulbus Allii Cepae is a kind of characteristic vegetable that integrates nutrition, medical treatment and health care, is described as the America and Europe " vegetable queen ".Contain numerous active substances such as volatile oil, flavone in the Bulbus Allii Cepae, have that antiinflammatory is antibacterial, prevention chronic bronchitis, treatment edema, suffer from diarrhoea, eliminate the phlegm, physiological functions such as diuresis, blood sugar lowering and angiocardiopathy preventing.Will provide new material for the development and use of slow down aging, antioxidation, blood sugar lowering, blood fat reducing, antithrombotic, natural drug such as anticancer to the further investigation of Bulbus Allii Cepae, for human health is contributed.
For a long time, opinions vary to old and feeble mechanism for scientists; In numerous causes of senescence, paid attention to most with free radical theory, people generally believe at present: aging mainly is because the normal defence of health and reparation hypofunction cause.According to the free-radical theory of aging, the reactive oxygen free radical (ROS), the especially ultra-oxygen anion free radical (O that in normal organism metabolic processes, produce
2-) be cause aging and with the one of the main reasons of diseases associated with senescence.
Beautiful nematicide (Caenorhabditis elegans, C.elegans are called for short nematicide) has many advantages as the model animal of studying anti-aging effects in the body, as easy cultivation, breeding soon; With integral body is object, and size of experiment amplifies easily, the nematicide health is transparent; Characteristics such as the h and E background relevant with its life-span is more clear, be fit to carry out the evaluation and the Mechanism Study of old and feeble intervention effect, it is combined with Chinese medicine, traditional food and the natural product of China, and making its instrument that becomes function/health food exploitation is the task of top priority.Medicinal herb components is extremely complicated, and natural micromolecular compounds such as various alkaloidss, glycoside, ketone, its biological activity are identified and can be utilized the nematicide of low-cost high-efficiency as evaluation model fully.
At present, in the patent report of relevant Bulbus Allii Cepae, focus mostly in onion essential oil and the research of Bulbus Allii Cepae recipe, do not find the nematicide life-span to be influenced the patent of aspect as yet about the Bulbus Allii Cepae flavone extract.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, the preparation method of a kind of purity height, the simple Bulbus Allii Cepae extract of preparation method is provided and prolongs the application of nematicide in the life-span and the food that comprises the Bulbus Allii Cepae extraction at it.
The objective of the invention is to be achieved through the following technical solutions:
A kind of preparation method of Bulbus Allii Cepae extract is characterized in that: step is as follows:
(1) gets Bulbus Allii Cepae, chopping, oven dry, pulverizing, mistake 60-80 mesh sieve, get the Bulbus Allii Cepae dried powder;
(2) be 1 by the Bulbus Allii Cepae powder with the extractant ratio: 15-20g/mL reflux, extract, 2-3 time, extraction temperature 65-75 ℃, lixiviate 2-4h in leaching process, intermittently stirs raw material 2-3 time every 0.5h, and each 3-5min leaches extracting solution and merging after the extraction;
(3) extracting solution is concentrated into the 5%--15% of cumulative volume, activated carbon decolorizing filters; With the petroleum ether extraction of 2-3 times of volume, reuse 2-3 times of volumes of acetic acid ethyl ester extracts 2-5 time earlier, and merging filtrate concentrates, and is dried to powder;
(4) aqueous sulfuric acid with 60-70 times of powder volume carries out back hydrolysis 1.5--2h, concentrates, and the concentrate feed liquid pump that obtains is gone on the polyamide adsorption column, goes up the sample flow velocity with 1mL/min, and absorption 30min removes impurity with the distilled water wash of 3-5 times of volume; Use the 75%--85% ethanol elution, elution flow rate 1mL/min collects ethanol elution; Concentrating under reduced pressure obtains Bulbus Allii Cepae extract.
And described extractant is: ethanol.
And the concentration of described aqueous sulfuric acid is 3% (v/v).
And the purity of Quercetin is 80%--95% in the described Bulbus Allii Cepae extract.
Bulbus Allii Cepae extract is prolonging the application of nematicide in the life-span.
A kind of food is characterized in that: food comprises Bulbus Allii Cepae extract.
And described food comprises food, health product, beverage and food additive.Advantage of the present invention and good effect are as follows:
1. the Bulbus Allii Cepae extract of the present invention's preparation has considerable influence to the nematicide life-span, wherein 30 μ g/mL Bulbus Allii Cepae extract (containing Quercetin more than 80%) can the beautiful nematicide life-span of significant prolongation and maximum life, can significantly improve the survival rate of nematicide SOD, CAT expression and heat shock experiment, be better than the action effect of vitamin E.
2. the invention provides the preparation method of a kind of purity height, the simple Bulbus Allii Cepae extract of preparation method, extract forms the Bulbus Allii Cepae extract lyophilized powder through lyophilizing, detects through HPLC, and main component is that Quercetin and purity reach 80%-95%.
3. the Bulbus Allii Cepae extract of the present invention's preparation can be prepared into slow down aging medicine, health product or food additive, can be made into various ways such as tablet, powder, granule, capsule, oral liquid, unguentum, the product of making comprises medicine, food or health product and batching thereof etc., and is of many uses.
Description of drawings:
Fig. 1 is the bar diagram of the Bulbus Allii Cepae extract of variable concentrations of the present invention to the influence of nematicide average life;
Fig. 2 is the nematicide survival curve figure of the embodiment of the invention 1 preparation;
Fig. 3 is the influence of Bulbus Allii Cepae extract of the present invention to nematicide SOD expression;
Fig. 4 is the influence of Bulbus Allii Cepae extract of the present invention to nematicide CAT expression;
Fig. 5 is the influence of Bulbus Allii Cepae extract of the present invention to the nematicide survival rate.
The specific embodiment
Below in conjunction with embodiment, the present invention will be further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
One, the preparation method of Bulbus Allii Cepae extract, step is as follows:
(1) accurately take by weighing fresh Bulbus Allii Cepae and clean, be cut into threadly, oven dry (55-65 ℃) in baking oven is pulverized with pulverizer, crosses the 60-80 mesh sieve, dried powder;
(2) be 1 with Bulbus Allii Cepae by the ratio of raw material and extractant (ethanol): 15-20 (g/ml) mixes, reflux, extract, 2-3 time, extraction temperature 65-75 ℃, extraction time 2-4h, in leaching process, intermittently stir raw material 2-3 time every 0.5h, each 3-5min, leaching process finish the after-filtration out-feed liquid and merge;
(3) extracting solution is put into the Rotary Evaporators concentrating under reduced pressure, be concentrated into 10% of cumulative volume, the extractant recycling, activated carbon decolorizing filters; Earlier use petroleum ether extraction, the weeding of grease soluble substance, reuse ethyl acetate extraction 3 times, merging filtrate concentrates after drying to powder;
(4) 3% (v/v) aqueous sulfuric acid with 60-70 times of volume carries out back hydrolysis 1.5-2h, and rotary evaporation pumps into the feed liquid that obtains on the polyamide adsorption column again, goes up the sample flow velocity with 1mL/min, and absorption 30min removes impurity with the distilled water wash of 3-5 times of volume; Use 80% ethanol elution, elution flow rate 1mL/min collects ethanol elution; Collect ethanol elution with the Rotary Evaporators concentrating under reduced pressure, reclaim solvent, obtain Bulbus Allii Cepae extract, the cryodesiccated Bulbus Allii Cepae extract lyophilized powder of concentrated solution;
(5) obtain the Bulbus Allii Cepae extract lyophilized powder after the lyophilization, detect through HPLC, main component is a Quercetin, and purity reaches 80%-95%.
Two, Bulbus Allii Cepae extract is in the application that prolongs the nematicide life-span
Experimental technique: reference literature (Lakowski B, Hekimi S.The genetics of caloric restriction inCaenorhabditis elegans, 1998,95:13091-13096) etc. method is measured the influence of Bulbus Allii Cepae extract to the nematicide life-span.
Life experiment: the nematicide behind the synchronization placed contain or do not contain in the NGM plate that is tried thing, the nematicide that grows for the L4 after date changes in the respective concentration brassboard, is designated as life experiment day 0 this moment; Observe nematicide every other day, and record nematicide existence, dead and eliminate the bar number of experiment.To the dead criterion of nematicide: do not have and move and swallowing act, still do not have any reaction after touching.Rejecting standard: 1. flee to the plate wall or cover and thirst; 2. worm's ovum is hatched in vivo and is formed a bag sample worm; 3. pierce in the agar.Contain 12.5 μ g/mL 5-Fu and variable concentrations in the brassboard and tried thing.DMSO is final concentration＜1% in experiment.All brassboard places 20 ℃ to cultivate down.Experimental concentration is respectively 10 μ g/mL, 30 μ g/mL, 50 μ g/mL; Matched group is a vitamin E.
Statistical analysis: experiment all repeats more than 2-3 time, adopts SPSS16.0 software to carry out the one factor analysis of variance experimental result.
The variable concentrations Bulbus Allii Cepae extract prolonged statistical table to the beautiful nematicide of the wild type life-span when table 1 was 20 ℃.
Table 1 variable concentrations Bulbus Allii Cepae extract prolongs statistical table (20 ℃) to the beautiful nematicide of the wild type life-span
Annotate:
★Maximum life is the average life of the last existence 10% of colony, rounds up during calculating; Compare with the blank group:
*P＜0.01,
*P＜0.05; Compare with the VE group:
△P＜0.05
As can be seen from Table 1, Bulbus Allii Cepae extract can prolong the life-span of Caenorhabditis elegans, and Bulbus Allii Cepae extract is compared with the vitamin E with dosage, and its life-span prolongation and antioxidant effect are more remarkable, and both have compared significant difference (P＜0.05)
Three, Bulbus Allii Cepae extract is to the influence of nematicide SOD, CAT content
SOD, CAT Determination on content: in nematode culture medium, add relative medicine, cultivate 48h for 20 ℃, the centrifugal 2-5min of 1200r/min collects nematicide, supernatant discarded, and polypide continues with the M9 buffer solution for cleaning of pre-cooling 3-5 time to remove larva and attached to the medicine in polypide and the intestinal wall; With the M9 buffer with the nematicide standardize solution to 300 μ L, move in the homogenizer, in ice bath, carry out homogenate.Homogenate is settled to 500 μ L, adds 1%Chaps buffer to 1mL, places 5min in ice bath, and 4 ℃, the centrifugal 10min of 10000r/min collects supernatant, and 4 ℃ of preservations are standby; Measure SOD, CAT content according to measuring test kit (bio-engineering corporation is built up in Nanjing) description again.
Experimental result shows that Bulbus Allii Cepae extract significantly improves nematicide SOD, CAT expression, has good antioxidation and/or defying age effect as shown in Figure 3, Figure 4.
Four, nematicide heat shock experiment
Experimental technique: synchronization is cultivated nematicide to the L4 phase under normal condition of culture, with the hermaphroditism wild type nematicide picking of some in nematicide growth medium NGM brassboard, the NGM brassboard is respectively Bulbus Allii Cepae extract group (concentration is 30 μ g/mL), VE matched group (concentration is 25 μ g/mL) and blank group, brassboard is put into 35 ℃ of incubators cultivate.Every the number of the survival of 1h record nematicide, death, rejecting, all dead until nematicide, the same life experiment of death standard.Experiment repeats 2-3 time, averages as end product.
Experimental result shows that Bulbus Allii Cepae extract has the activity of resisting oxidation free radical as shown in Figure 5, can significantly improve the survival rate of nematicide, has the effect that prolongs the nematicide life-span, and effect obviously is better than VE, and has significant difference (p＜0.05).
Five, the food that comprises Bulbus Allii Cepae extract
The Bulbus Allii Cepae extract of the present invention's preparation can be prepared into slow down aging medicine, health product or food additive, can be made into various ways such as tablet, powder, granule, capsule, oral liquid, unguentum, the product of making comprises medicine, food or health product and batching thereof etc., and is of many uses.
Claims (7)
1. the preparation method of a Bulbus Allii Cepae extract, it is characterized in that: step is as follows:
(1) gets Bulbus Allii Cepae, chopping, oven dry, pulverizing, mistake 60-80 mesh sieve, get the Bulbus Allii Cepae dried powder;
(2) be 1 by the Bulbus Allii Cepae powder with the extractant ratio: 15-20g/mL reflux, extract, 2-3 time, extraction temperature 65-75 ℃, lixiviate 2-4h in leaching process, intermittently stirs raw material 2-3 time every 0.5h, and each 3-5min leaches extracting solution and merging after the extraction;
(3) extracting solution is concentrated into the 5%--15% of cumulative volume, activated carbon decolorizing filters; With the petroleum ether extraction of 2-3 times of volume, reuse 2-3 times of volumes of acetic acid ethyl ester extracts 2-5 time earlier, and merging filtrate concentrates, and is dried to powder;
(4) aqueous sulfuric acid with 60-70 times of powder volume carries out back hydrolysis 1.5--2h, concentrates, and the concentrate feed liquid pump that obtains is gone on the polyamide adsorption column, goes up the sample flow velocity with 1mL/min, and absorption 30min removes impurity with the distilled water wash of 3-5 times of volume; Use the 75%--85% ethanol elution, elution flow rate 1mL/min collects ethanol elution; Concentrating under reduced pressure obtains Bulbus Allii Cepae extract.
2. the preparation method of Bulbus Allii Cepae extract according to claim 1, it is characterized in that: described extractant is: ethanol.
3. the preparation method of Bulbus Allii Cepae extract according to claim 1, it is characterized in that: the concentration of described aqueous sulfuric acid is 3% (v/v).
4. the preparation method of Bulbus Allii Cepae extract according to claim 1, it is characterized in that: the purity of Quercetin is 80%--95% in the described Bulbus Allii Cepae extract.
5. Bulbus Allii Cepae extract is prolonging the application of nematicide in the life-span.
6. food, it is characterized in that: described food comprises Bulbus Allii Cepae extract.
7. a kind of food according to claim 6 is characterized in that: described food comprises food, health product, beverage and food additive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100533411A CN102119993B (en) | 2011-03-07 | 2011-03-07 | Preparation method of onion extract, |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100533411A CN102119993B (en) | 2011-03-07 | 2011-03-07 | Preparation method of onion extract, |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102119993A true CN102119993A (en) | 2011-07-13 |
| CN102119993B CN102119993B (en) | 2012-05-23 |
Family
ID=44248721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100533411A Expired - Fee Related CN102119993B (en) | 2011-03-07 | 2011-03-07 | Preparation method of onion extract, |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102119993B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104693833A (en) * | 2015-03-30 | 2015-06-10 | 姚红亮 | Preparation method of onion skin vegetable dye |
| CN105794871A (en) * | 2016-04-29 | 2016-07-27 | 山东胜伟园林科技有限公司 | Insecticide containing onion extract and preparation method of insecticide |
| CN107653675A (en) * | 2017-11-13 | 2018-02-02 | 湖州佳伟纺织品有限公司 | Textiles antibacterial finishing agent and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1393241A (en) * | 2001-06-28 | 2003-01-29 | 南宁市合力科学技术研究所 | Onion extract and its preparing process |
| CN1443485A (en) * | 2003-04-15 | 2003-09-24 | 金海珠 | Functional onion beverage and its production method |
| CN101411499A (en) * | 2008-11-20 | 2009-04-22 | 南京农业大学 | Compositional soft capsule containing composite garlic and onion essential oil as well as anti-oxidant vitamins |
-
2011
- 2011-03-07 CN CN2011100533411A patent/CN102119993B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1393241A (en) * | 2001-06-28 | 2003-01-29 | 南宁市合力科学技术研究所 | Onion extract and its preparing process |
| CN1443485A (en) * | 2003-04-15 | 2003-09-24 | 金海珠 | Functional onion beverage and its production method |
| CN101411499A (en) * | 2008-11-20 | 2009-04-22 | 南京农业大学 | Compositional soft capsule containing composite garlic and onion essential oil as well as anti-oxidant vitamins |
Non-Patent Citations (3)
| Title |
|---|
| 《中国粮油学报》 20061231 刘焕云等 《溶剂法提取洋葱油的工艺研究》 123-125 1-7 第21卷, 第6期 2 * |
| 《国外医学中医中药分册》 20001231 Yang Xiaohong 《从洋葱中分离的黄酮类物质》 355-356 1-7 第22卷, 第6期 2 * |
| 《食品工业科技》 20100831 廖春龙等 《洋葱化学成分、生理保健功能和我国洋葱加工现状与发展趋势》 409-413 1-7 第31卷, 第8期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104693833A (en) * | 2015-03-30 | 2015-06-10 | 姚红亮 | Preparation method of onion skin vegetable dye |
| CN105794871A (en) * | 2016-04-29 | 2016-07-27 | 山东胜伟园林科技有限公司 | Insecticide containing onion extract and preparation method of insecticide |
| CN107653675A (en) * | 2017-11-13 | 2018-02-02 | 湖州佳伟纺织品有限公司 | Textiles antibacterial finishing agent and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102119993B (en) | 2012-05-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102132872B (en) | Health care food and application thereof | |
| CN101828593A (en) | Preparation method of composite green health food of freeze dried purslane and purslane/seaweed | |
| CN101775349B (en) | Health-care vinegar, brewing method and hyperglycemic reduction application thereof | |
| KR101298568B1 (en) | Anti-obesity food composition containing extract of arctium lappa l | |
| CN102119993B (en) | Preparation method of onion extract, | |
| CN104367987B (en) | Formulation of astragalus root for animals and preparation method thereof | |
| KR100803998B1 (en) | Fermented extract of Citrus Sunkii Hort, Method for processing thereof, and the healthy and funtional foods | |
| CN105218695A (en) | A kind of lycium ruthenicum polysaccharide extract and preparation method thereof | |
| CN101264094A (en) | Incarvillea younghusbandii phenylpropanoid glycoside composition and preparation and use thereof | |
| CN106421208B (en) | Pharmaceutical composition with chemical liver injury resistance function and preparation method thereof | |
| CN103536622B (en) | Sargassum fusiforme (Harv.) Setch sulfated polysaccharide is preparing the application in defying age medicine and health product | |
| CN101423558B (en) | Eupatorium adenophorum spreng polysaccharide and preparation method and use | |
| KR101344054B1 (en) | Composition for improving liver function containing fermented liquor of Hovenia dulcis Thunb extract as effective component | |
| CN101638444B (en) | Preparing process and usage of kostelezkya virginica polysaccharose | |
| CN108142790A (en) | Mental-tranquilization wheat flour | |
| CN108434171A (en) | A kind of arrowhead polysaccharide composition and preparation method thereof preventing chemical damage | |
| CN113925081A (en) | Natural plant anticorrosion composition, preparation method and application thereof | |
| CN104688958A (en) | Drug combination having function of losing weight | |
| CN101596268B (en) | Application of garlic total saponin in preparing medicaments and foods for resisting oxidative stress damage | |
| CN106172255B (en) | A kind of method for improving leech A prime content in leech body | |
| CN110664834A (en) | Application of tartary buckwheat polysaccharide in preparation of medicine for treating non-alcoholic fatty liver disease | |
| CN104522794B (en) | Antrodia camphorata detoxifying beverage and preparation method thereof | |
| CN107753659B (en) | Wei mountain Maojian tea tree extract and preparation method and application thereof | |
| CN107582606A (en) | The preparation method and its antioxidation application of beans taro ethanol extract | |
| CN1204140C (en) | Cistanche saponin and its extraction process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120523 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |