CN102084904A - Preparation technology for fu brick tea flowering loosing tea - Google Patents
Preparation technology for fu brick tea flowering loosing tea Download PDFInfo
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- CN102084904A CN102084904A CN 201010598870 CN201010598870A CN102084904A CN 102084904 A CN102084904 A CN 102084904A CN 201010598870 CN201010598870 CN 201010598870 CN 201010598870 A CN201010598870 A CN 201010598870A CN 102084904 A CN102084904 A CN 102084904A
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Abstract
The invention relates to a preparation technology for fu brick tea flowering loosing tea, which comprises the following steps of: (1) purifying and separating Eurotium Cristatum; (2) preparing and determining the concentration of the Eurotium Cristatum spore suspension; (3) processing dark green tea sample; (4) adding the Eurotium Cristatum spore suspension; (5) baking the resulting tea sample; (6) flowering under the control of humidity and temperature; (7) drying and preserving loosing tea. The invention successfully develops a technology condition for fu brick tea flowering, which enables the fu brick tea to flower in a short period of 3 to 4 days. In addition, the golden flowers are generally lush and have brilliant and golden color and large particles.
Description
Technical field:
The present invention relates to technical field of agricultural product process, be specifically related to a kind of Fu brick tea loose tea preparation technology that grows dim, described flower is the coronoid process capsule bacterium that looses, and is commonly called as golden flower.
Background technology
The Fu brick tea is the indispensable important beverage of borderland ethnic group.Golden flower is generally luxuriant, lovely luster is golden yellow, particle is loose is the important quality feature of high-quality Fu brick tea.The golden flower stage of growing dim in Fu brick tea process forms.In the past, the technical research of growing dim just rests on the compressed tea technology to the Fu brick tea, and is specifically less to the loose tea research data that grows dim.Thereby demand developing the concrete process conditions that a kind of Fu brick tea loose tea grows dim urgently.
Summary of the invention:
Technical problem to be solved by this invention is: solves the problem that above-mentioned prior art exists, and a kind of Fu brick tea loose tea preparation technology that grows dim is provided, can realize in a short time growing dim at 3-4 days, and golden flower is generally luxuriant, and bright in colour golden yellow, the particle hypertrophy.
The technical solution used in the present invention is: this Fu brick tea loose tea step of preparation process that grows dim is:
1) purifies and separates of the diffusing capsule bacterium of coronoid process;
2) coronoid process looses, and capsule bacterium spore suspension prepares and concentration is determined;
3) processing of black wool tea sample;
4) add the diffusing capsule bacterium spore suspension of coronoid process;
5) tea curing;
6) controlled humidity and temperature are grown dim;
7) the dry and preservation of loose tea.
In the technique scheme, the purifies and separates of the diffusing capsule bacterium of described coronoid process is: with the PDA agar solid medium that adds 10% tea juice; Coronoid process looses, and capsule bacterium spore suspension prepares and concentration is determined: with the PDA fluid nutrient medium that adds 10% tea juice, 20% sucrose and 10%NaCl; Spore suspension concentration determines to be controlled at 10
7-10
8Individual/mL; Being treated to of black wool tea sample: controlled humidity is about 25%; The diffusing capsule bacteria suspension addition of coronoid process is 0.25mL/100g; The tea curing condition is airtight 65-68 ℃, 1 hour; Controlled humidity and the temperature condition of growing dim are: a few days ago humidity 80%, temperature are 26 ℃, then 28 ℃ of humidity 70%, temperature until golden flower grow generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong; Loose tea 40-50 ℃ drying is the indoor preservation of normal temperature after 1 day.
PDA refers to the abbreviation of Potato Dextrose Agar, and Chinese implication is: potato dextrose agar.
PDA agar solid medium refers to potato is cleaned peeling, takes by weighing 200g, is cut into small pieces and puts into beaker, add an amount of distilled water, boiled 1 hour, filter with double gauze cold slightly back, adds 15g agar in filtrate, heat fused, add sucrose 20g at last, mixing is settled to 1000mL with graduated cylinder, be divided in while hot in the triangular flask, at 1.1kg/cm
2Keep sterilization in 20 minutes in (121 ℃).
The PDA fluid nutrient medium refers to above-mentioned culture medium process for preparation operation, but does not add agar.
The PDA flat board refers to be cooled to about 50 ℃ after above-mentioned medium sterilization finishes, and culture medium is poured in the also dry culture dish of having sterilized in advance into the PDA agar solid medium flat board that makes that is to be cooled on superclean bench.
Improvement PDA solid medium refers to potato is cleaned peeling, takes by weighing 200g, is cut into small pieces and puts into beaker, add an amount of distilled water, boiled 1 hour, filter with double gauze cold slightly back, adds the tea juice that 100mL prepares in filtrate, add 15g agar then, heat fused adds sucrose 20g mixing at last, is settled to 1000mL with graduated cylinder, be divided in while hot in the triangular flask, at 1.1kg/cm
2Keep sterilization in 20 minutes in (121 ℃).Sterilization is cooled to about 50 ℃ after finishing, and the PDA agar solid medium of 10% tea juice is poured in the also dry culture dish of having sterilized in advance into the PDA agar solid medium flat board that is the 10% tea juice that makes to be cooled on superclean bench.
Dull and stereotyped gradient dilution method is:
Finished product Fu brick sample is pulverized sample with the plant micropulverizer, and 200 orders sieve, 65 ℃ of dryings take by weighing the 1g sample after 2 hours, and constant volume 10mL promptly is made into 1 * 10
-1The Fu brick sample solution of g/mL is got 1mL constant volume 10mL and is made into 1 * 10 from above-mentioned solution
- 1The Fu brick sample solution of g/mL is mixed with concentration 1 * 10 successively
-3, 1 * 10
-4, 1 * 10
-5Each 10mL of Fu brick tea sample solution of g/mL is standby.Get 1 * 10
-3, 1 * 10
-4, 1 * 10
-5Each 0.5mL of Fu brick sample solution of g/mL is applied on the quick isolation medium flat board of crown diffusing capsule bacterium with spreader, dries up on the super-clean bench about 15 minutes, is inverted plate, 28 ℃ of cultivations, and picking dominant bacteria after 3-4 days---golden flower similarity condition is cultivated until purifying.
The setting-out inoculation refers to separate corresponding microorganism with the bacterium colony picking that it is good that the transfer needle of sterilization will be grown in new solid medium plate setting-out on super-clean bench.
The golden flower bacterium colony refers to that the golden flower bacterium grows into a bacterium colony aggregate that forms certain the time on the 10%PDA culture medium flat plate, and bacterium colony is meant the microbic mass that flocks together.
The concrete preparation method of tea juice: take by weighing 20g Fu brick tea according to 1g Fu brick tea sample than the ratio of 30mL distilled water, add 600mL distilled water, 100 ℃ of water-baths 2 hours are treated to cool off slightly the back and are settled to 600mL with graduated cylinder and are the tea juice for preparing.
Be made into 10
-1, 10
-2, 10
-3, 10
-4, 10
-5Be meant concentration, unit is g/mL.Spore concentration at outstanding night 10
7-10
8" individual " among individual/ml is meant the ascospore of single golden flower bacterium.Method of counting is counted or with its OD value of 722 type spectrophotometer measurements, is controlled at about 0.6-0.8 for being coated on the gradient dilution method to grow on the PDA agar solid medium flat board that adds 10% tea juice, makes golden flower spore suspension concentration be controlled at every milliliter 10
7-10
8Individual golden flower spore, the OD value is a light absorption value.
The invention provides a kind of can growing dim, and the grow dim fermentation process of loose tea of the Fu brick tea that golden flower is generally luxuriant, lovely luster is golden yellow, particle is loose, greatly improved the grow dim quality of loose tea of Fu brick tea 3-4 days realization in a short time.
Description of drawings:
Fig. 1 separated the diffusing capsule bacterium colony photo of coronoid process of purifying in 7 days for the present invention;
Fig. 2 is 7 days cultivation results photos of spore suspension of the diffusing capsule bacterium of coronoid process of the present invention;
Fig. 3 is the superfine product Fu brick tea black wool tea raw material of the loose tea photo as a result that grows dim that grows dim;
Fig. 4 is that the product Fu brick tea black wool tea raw material of the loose tea result that grows dim that grows dim examines photo;
Fig. 5 is the common people Fu brick tea black wool tea raw material of the loose tea photo as a result that grows dim that grows dim;
Fig. 6 is the superfine product Fu brick tea semi-finished product loose tea raw material of the loose tea photo as a result that grows dim that grows dim;
Fig. 7 is the product Fu brick tea semi-finished product loose tea raw material of the loose tea photo as a result that grows dim that grows dim;
Fig. 8 is the common people Fu brick tea semi-finished product loose tea raw material of the loose tea photo as a result that grows dim that grows dim;
The specific embodiment
1, the separation of golden flower, purifying:
The diffusing capsule bacterium of coronoid process is that golden flower separates from Yiyang tea processing factory finished product Hunan benefit board Fu brick sample.Concrete separate and purge process is: finished product Hunan benefit board Fu brick sample is pulverized, with dull and stereotyped gradient dilution method on the PDA flat board of interpolation 10% tea juice 28 ℃ of cultivations until purifying.
2, the preparation of the spore suspension of golden flower is determined with concentration:
The golden flower bacterium colony of picking purifying is seeded to and adds in the improvement PDA fluid nutrient medium, and 28 ℃ leave standstill cultivation 2 days, and the temperature that raises then makes spore suspension concentration be controlled at 10 to 30-32 ℃ of cultivation 7-10 days
7-10
8Individual/mL.
3, the processing of black wool tea sample:
Steam 10 minutes softening black wool tea with high-temperature steam, controlled humidity is 24% simultaneously.
4, add the golden flower spore suspension:
According to the ratio that every 100g tea adds 0.25mL golden flower spore suspension, add mixing with the form of spraying.
5, tea curing:
65-68 ℃ in confined conditions of tea behind interpolation golden flower spore suspension and the mixing, baking 1 hour.
6, controlled humidity and temperature are grown dim:
Controlled humidity and the temperature condition of growing dim is 26 ℃ of a few days ago humidity 80%, temperature, then 28 ℃ of humidity 70%, temperature until golden flower grow generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong.
7, the dry and preservation of loose tea:
Loose tea 40-50 ℃ drying is after 1 day, the indoor preservation of normal temperature.
Embodiment 1: the separation of golden flower, purifying:
Finished product Hunan benefit board Fu brick tea sample is pulverized with the plant micropulverizer, and 200 orders sieve, 65 ℃ of dryings take by weighing the 1g sample after 2 hours, and constant volume 10mL is made into 10
-1, 10
-2, 10
-3, 10
-4, 10
-5Each 10mL gets 10
-3, 10
-4, 10
-5The PDA flat board of 10% tea juice is added in three each 0.0.5mL coatings of gradient, dries up on the super-clean bench, cultivated 3 days for 28 ℃, and the golden yellow dominant colony of picking, setting-out is to the PDA flat board that adds 10% tea juice, and similarity condition continues to cultivate until purifying.
The golden flower bacterium colony of separation and purification is seen accompanying drawing 1.
Embodiment 2: the spore suspension preparation of golden flower:
The golden flower bacterium colony of picking purifying, be seeded in the PDA fluid nutrient medium that adds 10% tea juice, 20% sucrose, 10%NaCl, 28 ℃ leave standstill cultivation 2 days, the temperature that raises then was to 30-32 ℃ of cultivation 7-10 days, back 280rpm shaken cultivation is disperseed conidium again, uses the double-layer sterile filtered through gauze, and recording the OD value with 722 type spectrophotometers is 0.6-0.8, be available golden flower spore suspension, the OD value is light absorption value.
The spore suspension cultivation results of golden flower is seen accompanying drawing 2.
Embodiment 3: the checking that the black wool tea loose tea of the superfine product Fu of Yiyang tea processing factory brick, a product Fu brick, common people Fu brick grows dim:
Get each 4Kg of black wool tea of the superfine product Fu of Yiyang tea processing factory brick, a product Fu brick, common people Fu brick respectively, carry out loose tea according to the 2-7 step in the specific embodiment and ferment.
Black wool tea loose tea grows dim and the results are shown in accompanying drawing 3, Fig. 4, Fig. 5.
Embodiment 4: the checking that the semi-finished product loose tea of the corresponding screening of the superfine product Fu of Yiyang tea processing factory brick, a product Fu brick, common people Fu brick, fermentation, assorted preparation compacting grows dim:
Get each 4Kg of semi-finished product of corresponding screening, the fermentation of the superfine product Fu of Yiyang tea processing factory brick, a product Fu brick, common people Fu brick, assorted preparation compacting respectively, carry out the loose tea fermentation according to the 2-7 step in the specific embodiment.
The semi-finished product loose tea grows dim and the results are shown in accompanying drawing 6, Fig. 7, Fig. 8.
Claims (2)
1. Fu brick tea loose tea preparation technology that grows dim is characterized in that step is:
1) purifies and separates of the diffusing capsule bacterium of coronoid process;
2) coronoid process looses, and capsule bacterium spore suspension prepares and concentration is determined;
3) processing of black wool tea sample;
4) add the diffusing capsule bacterium spore suspension of coronoid process;
5) tea curing;
6) controlled humidity and temperature are grown dim;
7) the dry and preservation of loose tea.
2. the Fu brick tea according to claim 1 loose tea preparation technology that grows dim is characterized in that: the loose purifies and separates of capsule bacterium of described coronoid process is: with the PDA agar solid medium that adds 10% tea juice; Coronoid process looses, and the capsule bacterium prepares spore suspension and concentration is determined: with the PDA fluid nutrient medium that adds 10% tea juice, 20% sucrose, 10%NaCl; Spore suspension concentration is controlled at 10
7-10
8Individual/mL; Being treated to of black wool tea sample: controlled humidity is about 25%; The diffusing capsule bacteria suspension addition of coronoid process is 0.25mL/100g; The tea curing condition is airtight 65-68 ℃, 1 hour; Controlled humidity and the temperature condition of growing dim are: a few days ago humidity 80%, temperature are 26 ℃, then 28 ℃ of humidity 70%, temperature until golden flower grow generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong; Loose tea 40-50 ℃ drying is the indoor preservation of normal temperature after 1 day.
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| CN102511579A (en) * | 2011-12-14 | 2012-06-27 | 杭州英仕利生物科技有限公司 | Black oolong tea and its preparation method |
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| CN102550722A (en) * | 2012-03-26 | 2012-07-11 | 孙建兵 | Fu brick tea and production method |
| CN102640814A (en) * | 2012-05-14 | 2012-08-22 | 咸阳泾渭茯茶有限公司 | Method for making golden flower loose tea without inoculation |
| CN102860373A (en) * | 2012-05-23 | 2013-01-09 | 华中农业大学 | Method for producing eurotium cristatum tea by utilizing fresh tea tree leaves |
| CN102860389A (en) * | 2012-05-23 | 2013-01-09 | 华中农业大学 | Lotus leaf eurotium cristatum tea and preparation method thereof |
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