CN102049057B - Method for killing bacterial spores - Google Patents
Method for killing bacterial spores Download PDFInfo
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- CN102049057B CN102049057B CN 201010593756 CN201010593756A CN102049057B CN 102049057 B CN102049057 B CN 102049057B CN 201010593756 CN201010593756 CN 201010593756 CN 201010593756 A CN201010593756 A CN 201010593756A CN 102049057 B CN102049057 B CN 102049057B
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Abstract
The invention relates to a method for killing bacterial spores. The method comprises the following steps: 1) dropping L-alanine serving as a spore germinator on a contaminated article; 2) then continuing dropping a strong alkaline solution serving as a resistance weakening agent; and 3) adjusting the pH value of the solution to 4.0-5.0 by taking H2O2, Fe2<+> and EDTA as a reaction system so as to prepare an oxidant; and dropping the prepared oxidant on the contaminated article. The method provided by the invention is used for disinfecting the contaminated environment and articles and killing the bacterial spores attached to the environment and articles, and has the advantages of good effect, low toxicity, little stimulus, low cost and the like.
Description
Technical field
The present invention relates to a kind of method of sterilization, particularly a kind of effectively method of deactivation bacterial spore.
Technical background
Bacterial spore has the resistant function of height to physical and chemical factor.Therefore, conventional means was to take to burn to add high concentration chemicals long period of soaking when decontamination was carried out in the plague area due to the bacterial spore, but these methods are very serious to the pollution of environment.Carried out on-the-spot anthrax test like near the Gruinard island nineteen forty-two Scotland seashore, island are seriously polluted, just are purified after adopting high-concentration formaldehyde to add the sea water mixing immersion treatment in 1986; 2003, what the anthrax grave of Three Gorge Reservoir Region in Chongqing was handled also employing was that high power amount bleaching powder and mixing with soil are soaked, then the method for gasoline burning.
The characteristics that attack according to bio-terrorism in recent years attack ground often in the place of crowd's high concentration, as build in interior, the means of transport and important traffic place, and on-the-spot decontamination difficulty increases with requiring; It is very necessary to set up situ purification treatment technology reliable, environment-friendly type.
Summary of the invention
The method that the purpose of this invention is to provide a kind of kill bacteria brood cell; Said method is utilized inducing of germinant and drag attenuator; Promote bacterial spore to start rudiment; Reduce the drag of cell space structure and composition, make that its chitin under the further effect of oxidant breaks, nucleolysis, thalline be by effective deactivation.The present invention is used for contaminated environment and sterilisation of objects, kills the bacterial spore that is attached on environment and the article, and has effectively, and toxicity is low, and zest is little, low cost and other advantages.
Technical scheme of the present invention is:
Kill bacteria brood cell's method has following steps:
1) on contaminated article, drip spill concentration be 0.10-0.40 L as the L-alanine of brood cell's germinant, effect 20-60min;
2) subsequently, on the contaminated article of handling through the described brood cell's germinant of step 1), continue to drip spill concentration be 0.02-0.50 L as the strong base solution of drag attenuator, effect 20-40min;
3) with H
2O
2, Fe
2+, EDTA is reaction system, wherein H
2O
2Concentration be 0.10-0.80mol/L; Fe
2+Concentration be 0.01-0.08mol/L; The concentration of EDTA is 0.01-0.08mol/L; Get isopyknic H
2O
2, Fe
2+, EDTA solution is mixed, the pH value of adjustment solution is 4.0-5.0, promptly gets the preparation oxidant of being prepared; The oxidant of getting preparation drips and is sprinkled upon step 2) on the described contaminated article, action time 30-60min.
Step 2) described strong base solution is strong base solutions such as sodium hydroxide or potassium hydroxide.
Step 3) is described; Fe
2+Be ferrous sulfate or solution of ferrous chloride.
Reagent described in brood cell's germinant of the present invention, drag attenuator (basifier) and the oxidant is the commercially available prod.
Germinant, drag attenuator mainly act on brood cell's chitin and brood cell's clothing of bacterial spore among the present invention, and it is damaged that it is produced, and eliminates barrier and protective effect, and brood cell's core exposes; Oxidant can produce the hydroxyl radical free radical with forceful electric power position, and oxidant has the forceful electric power position, therefore strong oxidation is arranged, and germination body nucleolysis, enzymatic activity are disappeared, and thalline thoroughly is inactivated.
The method that the present invention is used for the kill bacteria brood cell is hypotoxicity to animal, and corrosivity is little, cost is low, and is easy to use.Each constituent is preserved at room temperature and is got final product, good stability, during use with joining with usefulness.
The specific embodiment
Below in conjunction with instance the present invention is done further explanation.
1. results of laboratory
With final concentration be 0.10 L the L-alanine be the germinant main constituent, effect brood cell suspension 20-60min.Be that sodium hydroxide aqueous slkali mol the 0.02-0.50 is the drag attenuator with concentration, act on above-mentioned brood cell's suspension 20-40min, with H
2O
2, Fe
2+, EDTA is reaction system, confirms H
2O
2Final concentration be 0.20mol/L, the concentration of ferrous sulfate is 0.02mol/L, the concentration of EDTA is 0.02mol/L, pH value 4.0-5.0, action time 30-60min.The result is as shown in table 1.
Brood cell's germinant of the present invention, drag attenuator (basifier) and oxidant, during use according to this method compound method, and according to sterilization steps according to the invention with joining with usefulness.
2. in-site modeling sterilization experiment
Get stone, tile, blade, cloth sheet, wooden unit surface (1x1cm area) drip brood cell's suspension and drip 10ul brood cell's suspension, get sandy soil 0.05g and drip 10ul brood cell's suspension, simulate contaminated external environment.Carry out brood cell's inactivation treatment with the inventive method:
With final concentration be 0.40 L the L-alanine be germinant, drip 10ul in article surface, effect 20-60min;
Be that sodium hydroxide aqueous slkali mol the 0.12-0.25 is the drag attenuator with concentration, drip 90ul in above-mentioned article surface, effect 20-40min;
With H
2O
2, Fe
2+, EDTA is reaction system, confirms H
2O
2Final concentration be 0.20mol/L, the concentration of ferrous sulfate is 0.02mol/L, the concentration of EDTA is 0.02mol/L, pH value in reaction 4.0-5.0 drips 90ul in above-mentioned article surface, effect 30-60min.
The result is carried out count plate.Different analog sample brood cell deactivation results see table 2.
3. act on the mixed liquor toxicity test
Choose 60 of the SPF level Wistar rats of body weight 180-200 g, be divided into experimental group and matched group, male and female half and half.Acute oral toxicity test is pressed the 1.00ml/100g body weight, with gastric infusion of used mixed disinfecting liquid, observes 14d record body weight, diet, death condition continuously; Subacute per os toxicity test is pressed the 1.00ml/100g body weight, and every day, per os was irritated the stomach contamination, and negative control group gives sterile distilled water.Continuous contamination 28d, last contamination back 24h broken end is got blood, detects each item routine blood test index, and major organs is carried out pathologic finding.
The result: all in this detection unit range of normal value, each item index of each dose groups and matched group compare each item index, the equal not statistically significant of difference.This concentration mixed disinfecting liquid is nontoxic.
Claims (3)
1. a kill bacteria brood cell method is characterized in that, following steps are arranged:
1) on contaminated article, drips that to spill concentration be the L-alanine of 0.10-0.40 mol/L as brood cell's germinant, effect 20-60min;
2) subsequently, continue to drip on the contaminated article of handling through the described brood cell's germinant of step 1) that to spill concentration be the strong base solution of 0.02-0.50 mol/L as the drag attenuator, effect 20-40min;
3) with H
2O
2, Fe
2+, EDTA is reaction system, wherein H
2O
2Concentration be 0.10-0.80mol/L; Fe
2+Concentration be 0.01-0.08mol/L; The concentration of EDTA is 0.01-0.08mol/L; Get isopyknic H
2O
2, Fe
2+, EDTA solution is mixed, the pH value of adjustment solution is 4.0-5.0, promptly gets the oxidant of being prepared; The oxidant of getting preparation drips and is sprinkled upon step 2) on the described contaminated article, action time 30-60min.
2. kill bacteria brood cell's according to claim 1 method is characterized in that: step 2) described strong base solution is sodium hydroxide or potassium hydroxide.
3. kill bacteria brood cell's according to claim 1 method is characterized in that: the described Fe of step 3)
2+Be ferrous sulfate or solution of ferrous chloride.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010593756 CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010593756 CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102049057A CN102049057A (en) | 2011-05-11 |
| CN102049057B true CN102049057B (en) | 2012-12-12 |
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ID=43954071
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010593756 Expired - Fee Related CN102049057B (en) | 2010-12-17 | 2010-12-17 | Method for killing bacterial spores |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102701881B (en) * | 2012-06-05 | 2014-05-14 | 凌天骏 | Soil disinfectant and soil disinfecting and sterilizing method |
| CN105962002B (en) * | 2016-05-05 | 2019-07-09 | 中国农业科学院农产品加工研究所 | The method that mild formula kills bacterial spore |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6359004B1 (en) * | 1996-08-30 | 2002-03-19 | Duke University | Manipulating nitrosative stress to upregulate nitrosative stress defenses |
| CN1545883A (en) * | 2003-12-15 | 2004-11-17 | 韶山大北农动物药业有限公司 | Water soluble composite potassium monopersulfate powder disinfectant |
| JP4228175B2 (en) * | 2002-01-11 | 2009-02-25 | 三菱瓦斯化学株式会社 | Disinfectant composition liquid and disinfecting method |
| CN101698102A (en) * | 2009-05-13 | 2010-04-28 | 朱玛华 | Ultra-potent composite for decontaminating hazardous chemical substances |
-
2010
- 2010-12-17 CN CN 201010593756 patent/CN102049057B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6359004B1 (en) * | 1996-08-30 | 2002-03-19 | Duke University | Manipulating nitrosative stress to upregulate nitrosative stress defenses |
| JP4228175B2 (en) * | 2002-01-11 | 2009-02-25 | 三菱瓦斯化学株式会社 | Disinfectant composition liquid and disinfecting method |
| CN1545883A (en) * | 2003-12-15 | 2004-11-17 | 韶山大北农动物药业有限公司 | Water soluble composite potassium monopersulfate powder disinfectant |
| CN101698102A (en) * | 2009-05-13 | 2010-04-28 | 朱玛华 | Ultra-potent composite for decontaminating hazardous chemical substances |
Non-Patent Citations (1)
| Title |
|---|
| JP特许第4228175号B2 2009.02.25 |
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| Publication number | Publication date |
|---|---|
| CN102049057A (en) | 2011-05-11 |
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