CN102021204A - Poly-generation utilizing method for ester extraction, fermentation and classification of white spirit vinasse - Google Patents
Poly-generation utilizing method for ester extraction, fermentation and classification of white spirit vinasse Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
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- Y02W30/91—Use of waste materials as fillers for mortars or concrete
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Abstract
The invention discloses a poly-generation utilizing technological system for the ester extraction, the fermentation and the classification of white spirit vinasse, which gets rid of the traditional thought of utilizing a certain component of the vinasse or the whole vinasse as fuel and a feed, and transforms the vinasse into products with high additional value, such as a bouquet matter, alcohol, a protein feed, a raw material for making building materials, and the like. The method comprises the following basic steps of: firstly extracting the volatile matters in the vinasse, replacing the cooking with high pressure and long time by steam explosion with low pressure and short time, directly carrying out solid-state fermentation on the treated residual starch to generate alcohol, extracting the alcohol generated by the fermentation by using the cyclic gas stripping of carbon dioxide during the fermentation, mechanically classifying the fermented materials by a special apparatus, separating a part which is rich in protein and starch from a rice hull part, carrying out direct or aerobic solid-state fermentation on the separated high-protein part to further increase the protein content, drying and crushing the high-protein part to be used as a high-protein feed, and using the separated rice hull part as the raw material of building materials directly or after drying treatment.
Description
Technical field
The present invention relates to liquor and produce byproduct comprehensive and utilize method, particularly relate to a kind of distillers ' grains and put forward ester-fermentation-classification Poly-generation and utilize method.
Background technology
Liquor is the traditional beverage of China, and it was brewageed historical existing several thousand.Along with the industrialization that liquor is produced, the amount of its byproduct also increases day by day.China produces about 2,700 ten thousand tons of liquor per year, and the vinasse of generation reach more than 6,500 ten thousand tons, and amount is concentrated greatly.Because it is acid, very easily go mouldy, if untimely processing can cause serious pollution, and vinasse itself still have certain nutrient value and utilize part because fermentation not exclusively waits a variety of causes.Contain a large amount of fragrance matters in by product vinasse, yellow water and the residual water of liquor production in addition, as ethyl acetate, ethyl hexanoate, ethyl lactate, ethyl butyrate etc., brewery does not mostly effectively utilize it and directly it is abandoned, so both do not had economic benefit, brought environmental pollution again.
The water content of fresh vinasse is higher, is 60-70%.The main component of vinasse is rice husks, in addition, also contains residual starch 5-11%, crude protein 12-23%, and robust fibre 18-27%, crude fat 2-7% can carry out the diversification development and use.Simultaneously resources such as the residual sugar in the vinasse biomass, starch, abundant Mierocrystalline cellulose and hemicellulose cause the huge wasting of resources if can not obtain reasonable use.Therefore, fully utilize comprehensively vinasse, prevent to pollute, avoid waste, turn waste into wealth imperative.
At present China's vinasse are used for medical treatment mostly and go up high price materials such as extracting albumen etc., or directly are used for cultivating edible mushrooms, make feed, produce the elementary utilization of aspects such as biogas, utilize that level and utilization ratio are not high, added value is lower.As in the process that vinasse utilize, for the Ester in the vinasse, brewery does not have it is effectively utilized and directly it is abandoned substantially.And when utilizing vinasse to produce feed, because a large amount of rice husks that add in the liquor brew process, in general, the weight of rice husk is at 30-60% in the vinasse, volume accounts for 50-70%, and coarse-fibred content is more than 20%, and is low as feed digestibility, palatability is poor, be difficult to absorb, influence feeding effect.At present, vinasse mainly directly are used as the feed weighting material by near the peasant brewery and raiser, this mode of utilizing, and benefit is very low, is a kind of wasting of resources to a great extent.Also have rice husk is removed in vinasse oven dry back, separate the back as feed, but this method product processed added value is very low, and not high as feed nutritive value yet.The higher method of added value is that classification was the processing method that 99111606.2 patent of invention discloses a kind of producing vinasse somatic protein fodder by solide aerobic fermentation as protein fodder such as application number after vinasse were inoculated the secondary fermentation of certain micro-organisms bacterial classification and increase the protein content of vinasse.The at present domestic microorganism that is mainly used in the production tropina has Aspergillus, Rhizopus, candidiasis, lactobacillus, streptococcus acidi lactici, Bacillus subtilus, Methionin to produce bacterium, intends endomyces, geotrichum candidum etc.Though this method also has certain effect, burn often as vinasse exsiccant thermal source for the more rice husk of content in the vinasse.It seems generally, the above-mentioned mode of utilizing, the increment amplitude of vinasse is little, and these technology are single often, only noted a certain Products Development, in fact had only each composition of vinasse is realized effectively that all high-valued resource and the abundant vinasse problem thereof of just might making solved completely.
Summary of the invention
[goal of the invention] vinasse are significant by-products that liquor is produced.The generation of a large amount of vinasse needs corresponding means it is handled.Distillery can be divided into two kinds of means basically to the processing of vinasse at present, and the first is freely given near peasant or the feedlot roughage as animal with it as rubbish, and this mode is fairly simple, and engineering need not investment, but also is difficult to therefrom obtain income.Also having a kind of mode is to carry out simple dry and screening to vinasse, and a part of rice husk in the vinasse is sifted out as exsiccant fuel and rich in starch, and a proteinic part is as feed.Though this processing mode makes vinasse that the lifting that is worth arranged, appreciation space is little.Except that containing a large amount of rice husks, also contain ester class and residual starch 5-11% in the vinasse, crude protein 12-23%, robust fibre 18-27%, crude fat 2-7% can carry out the diversification development and use.Resources such as the ester class in the vinasse biomass, residual sugar, starch, abundant Mierocrystalline cellulose and hemicellulose cause the huge wasting of resources if can not obtain reasonable use.Therefore, fully utilize comprehensively vinasse, prevent to pollute, avoid waste, turn waste into wealth imperative.
The Ester that contains abundant generation wine body note gas in by product vinasse, yellow water and the residual water in [design of the present invention] liquor production, in traditional lees feeds utilization or rice husk incendiary process, these fragrance matters all can not get reclaiming; In addition, these aroma substances are volatile matter, and therefore, the recovery of Ester should be listed in the first step of vinasse comprehensive utilization.The rice husk that contains 30-60% in the vinasse, it is higher to contain ash oontent in the rice husk, and wherein mainly is silicon-dioxide, is the good raw material of making siliceous product.But except rice husk, also contain the residual starch of 8-12% in the vinasse, for these residual starches, the general method of utilizing is that it is being added the aerobic or anaerobically fermenting of a part of bacterial classification continuation, produce tropina, improve the protein content in the vinasse, as feed.Application number is the processing method that 99111606.2 patent of invention discloses a kind of producing vinasse somatic protein fodder by solide aerobic fermentation, the contriver inoculates geotrichum candidum in vinasse increase protein content in the vinasse, but for ferment effect, also need in vinasse, to add extra nutritive substances such as a certain proportion of wheat bran and ammonium sulfate, this has just increased processing cost, add single product, be difficult to make the value of vinasse to produce bigger increase.If vinasse by pre-treatment simple, low consumption, are added certain yeast, utilize residual amylofermentation ethanol, when obtaining alcohol product, further yeast fermentation broth has enriched proteic content in the vinasse.By above-mentioned analysis, in fact can four kinds of value products of minimum generation in the vinasse, design the production technique of product, just can make the resource component in the vinasse obtain fully utilizing.
[technical solution of the present invention] the objective of the invention is to realize by following steps:
(1) vinasse is packed in the quick-fried equipment of vapour of being with water distilling apparatus, spray into liquor fermentation by product yellow water or residual water, feed vapor distillation, reclaim volatile materials such as Ester in vinasse and the yellow water and ethanol;
(2) close water distilling apparatus, continue to feed steam, carry out the quick-fried processing of vapour when reaching the quick-fried condition of vapour;
(3) inoculation active dry yeast of the material after the quick-fried processing of vapour and saccharifying enzyme carry out ethanol fermentation in solid state fermentation or continuous solid-state fermentation reactor, and the ethanol that utilizes carbonic acid gas circulation gas to carry the fermentation generation in the time of fermentation proposes;
(4) material that comes out from fermentation reactor utilizes task equipment to carry out mechanical classification, and the part that is rich in albumen, starch is partly separated with rice husk;
(5) separating the direct or aerobic solid-state mixed fungus fermentation of protein part that obtains further increases the protein content after drying, pulverizes the back as high protein feed;
(6) the rice husk part that separate to obtain directly or after the drying treatment as the raw material of making material of construction.
The described feeding vapor distillation of step (1), distillation temperature is 40-180 ℃.
The quick-fried condition of the described vapour of step (2) is pressure 0.6-1.2MPa, dimension pressure time 1-50min.
The described active dry yeast of step (3) is according to the mass ratio 1 of yeast and material: 1000-1: 10000 ratio is added, and saccharifying enzyme is according to the mass ratio 1 of saccharifying enzyme and material: 1000-1: 5000 ratio interpolation.
The described stock grading specific equipment of step (4) is based on maize sheller, and the orifice plate of thresing machine bottom is replaced with 8 orders-14 purpose screen cloth.
The described mixed bacterium of step (5) is one or more in Penicillium decumbens, viride, koning trichoderma, whiterot fungi, geotrichum candidum, aspergillus niger, the Nocardia bacteria.
The described material of construction of step (6) is ash wall body plate, wall body brick, roofing board, ecological plate, floor plates.
The present invention has following characteristics:
At first with the water that needs in the vinasse to add to be that yellow water or the residual water that liquor is produced by product replaces equally, when reclaiming the ester class, make other by products obtain higher value application;
2. the quick-fried processing of vapour that vinasse are carried out low pressure (0.6-1.2MPa) short period of time (1-50min) makes that the starch that is difficult to utilize in the vinasse suitably decomposes, gelatinization, substituted long-time (0.5-2h) high temperature (100-120 ℃) digestion process of starch pasting process, cut down the consumption of energy greatly when increasing the yield of liquor of vinasse;
3. with the extraction of volatile matter such as ester class and ethanol and vapour is quick-fried finishes, reduce unit operation in same device, reduce facility investment;
4. volatile matter such as ester class and ethanol are reclaimed and place other vapour quick-fried, before the treatment step such as fermentation, take into full account its volatility, improve the utilization ratio of by product;
5. the back material that ferments is carried out the classification utilization, when having improved the protein fodder quality, make the rice husk in the vinasse be able to higher value application;
6. vinasse are carried out combined treatment, existing vinasse component is carried out abundant high-valued technology utilization, broken away from and only a certain component in the vinasse has been utilized or the whole conventional thought of utilizing, vinasse have been converted into the high value added products such as raw material of wine body note flavor material, ethanol, protein fodder and making material of construction.
Embodiment
The present invention will be further described below by embodiment.
Embodiment 1
Take from the vinasse of a Shanxi distillery, at first its component is measured.Measurement result shows that the water content of this vinasse is 60.7%, and the residual starch content of the vinasse of measuring with enzyme acid associating hydrolysis method is 10.1%, is 12.8% with the content of the crude protein of the vinasse of Kjeldahl determination mensuration, and the mass ratio of rice husk is 63%.
(1) gets the above-mentioned vinasse of 200kg,, spray into the yellow water of 40kg in the time of reinforced more uniformly at first with its steam-explosion jar that adds 0.5 cubic metre, feed hot steam and be warmed up to 80 ℃, reclaim the ester class, adopt GC7890-MS5973N type gas chromatograph-mass spectrometer, chromatographic condition: HP-INNOWAX chromatographic column (30m * 250 μ m * 0.25 μ m), adopt temperature programming: 45 ℃ of initial temperature, constant temperature 5min rises to 195 ℃ with 5 ℃ of per minutes, keeps 2min, be raised to 220 ℃ with 7 ℃ of per minutes, keep 8min to finish.220 ℃ of injector temperatures; Carrier gas is a high-pure helium, flow velocity 1.0ml/min; Sample size 1.0 μ L; Splitting ratio 10: 1.The mass spectrum condition: the ionization mode is electron-bombardment, electron energy 70eV, 220 ℃ of interface temperature, electron-multiplier voltage 1.89KV; Sweep limit 35-500u adopts the full scan mode of operation.According to the chromatogram assay, the rate of recovery of the fragrance matter in the vinasse is 67.3%.
(2) close water distilling apparatus, continue to feed hot steam, arrive the quick-fried condition of vapour material is carried out the quick-fried processing of vapour, the quick-fried treatment condition of vapour are: the 0.7MPa dimension presses 2.5min to carry out the quick-fried processing of vapour.
(3) material after the quick-fried processing of vapour utilizes the continuous solid-state fermentation of 120L and the product gas-stripping heat-pump isolating equipment that is coupled that the residual starch in the vinasse is carried out the yeast ethanol fermentation, active dry yeast adds according to 1: 1000 the ratio of mass ratio of yeast and material, and saccharifying enzyme adds according to 1: 5000 the ratio of mass ratio of saccharifying enzyme and material; The ethanol that utilizes carbonic acid gas circulation gas to carry the fermentation generation in the time of fermentation proposes; Alcoholic acid output is 4.3% of vinasse after testing.
(4) its water content of material of coming out from fermentation reactor has been reduced to about 31%.Use vinasse mechanical classification device that it is carried out classification, this sorting equipment is to be transformed into after the orifice plate of YTJ-1 type corn threshing motor spindle is replaced with 10 purpose Stainless Steel Cloths.Utilize this device, the revolution of regulating grading wheel is 2000 commentariess on classics/min, can easily the most rice husks in the vinasse be separated, isolating effect be the rice husk part account for 50% of gross weight, rice husk rice husk ratio partly is up to 85%.Almost cannot see complete rice husk in the protein part and be rich in.
(5) separate obtain be rich in protein part in solid-state fermenter further microbe inoculation carry out aerobic fermentation and improve its proteic content.Concrete grammar is, the water content of regulating material is 60%, Penicillium decumbens is carried out the two-stage liquid seed culture, seed liquor is inoculated according to 10% of material dry weight, put into solid-state fermenter after mixing thoroughly after the inoculation and carry out aerobic fermentation, fermenting process passes through the temperature of the Ventilation Control bed of material about 28-32 ℃, and fermentation time is 72h, cultivates material after the fermentation ends and covers with uniform mycelium.With cultured fermentation material 120 ℃ down dry, pulverize and promptly obtain crude protein content at 34.5% high protein feed.
(6) separating the rice husk part obtain can be through pulverizing, and extravagant, hot pressing such as cut at the ecological plate of explained hereafter.。
Embodiment 2
Take from the vinasse of a Shanxi distillery, at first its component is measured.Measurement result shows that the water content of this vinasse is 60.7%, and the residual starch content of the vinasse of measuring with enzyme acid associating hydrolysis method is 10.1%, is 12.8% with the content of the crude protein of the vinasse of Kjeldahl determination mensuration, and the mass ratio of rice husk is 63%.
(1) gets the above-mentioned vinasse of 200kg,, spray into the yellow water of 60kg in the time of reinforced more uniformly at first with its steam-explosion jar that adds 0.5 cubic metre, be warmed up to 40 ℃, begin to reclaim the ester class, adopt GC7890-MS5973N type gas chromatograph-mass spectrometer, chromatographic condition: HP-INNOWAX chromatographic column (30m * 250 μ m * 0.25 μ m), adopt temperature programming: 45 ℃ of initial temperature, constant temperature 5min rises to 195 ℃ with 5 ℃ of per minutes, keeps 2min, be raised to 220 ℃ with 7 ℃ of per minutes, keep 8min to finish.220 ℃ of injector temperatures; Carrier gas is a high-pure helium, flow velocity 1.0ml/min; Sample size 1.0 μ L; Splitting ratio 10: 1.The mass spectrum condition: the ionization mode is electron-bombardment, electron energy 70eV, 220 ℃ of interface temperature, electron-multiplier voltage 1.89KV; Sweep limit 35-500u adopts the full scan mode of operation.According to the chromatogram assay, the rate of recovery of the fragrance matter in the vinasse is 44.3%.
(2) close water distilling apparatus, continue to heat up, arrive the quick-fried condition of vapour material is carried out the quick-fried processing of vapour, the quick-fried treatment condition of vapour are: the 0.9MPa dimension presses 1min to carry out the quick-fried processing of vapour.
(3) material after the quick-fried processing of vapour utilizes the continuous solid-state fermentation of 120L and the product gas-stripping heat-pump isolating equipment that is coupled that the residual starch in the vinasse is carried out the yeast ethanol fermentation, active dry yeast adds according to 1: 10000 the ratio of mass ratio of yeast and material, and saccharifying enzyme adds according to 1: 1000 the ratio of mass ratio of saccharifying enzyme and material; The ethanol that utilizes carbonic acid gas circulation gas to carry the fermentation generation in the time of fermentation proposes; Alcoholic acid output is 2.3% of vinasse after testing.
(4) its water content of material of coming out from fermentation reactor has been reduced to about 30%.Use vinasse mechanical classification device that it is carried out classification, this sorting equipment is to be transformed into after the orifice plate of YTJ-1 type corn threshing motor spindle is replaced with 8 purpose Stainless Steel Cloths.Utilize this device, the revolution of regulating grading wheel is 2000 commentariess on classics/min, can easily the most rice husks in the vinasse be separated, isolating effect be the rice husk part account for 55% of gross weight, rice husk rice husk ratio partly is up to 85%.Almost cannot see complete rice husk in the protein part and be rich in.
(5) vinasse separate obtain be rich in protein part in solid-state fermenter further microbe inoculation carry out aerobic fermentation and improve its proteic content.Concrete grammar is, the water content of regulating material is 60%, the wooden enzyme of green, whiterot fungi and Ruo Ka Salmonella are carried out the two-stage liquid seed culture, seed liquor is inoculated according to 10% of material dry weight, put into solid-state fermenter after mixing thoroughly after the inoculation and carry out aerobic fermentation, fermenting process passes through the temperature of the Ventilation Control bed of material about 28-32 ℃, and fermentation time is 72h, cultivates material after the fermentation ends and covers with uniform mycelium.With cultured fermentation material 120 ℃ down dry, pulverize and promptly obtain crude protein content at 39.4% high protein feed.
(6) the rice husk part instead of part perlite material produce ash wall body plate that classification is obtained.
Embodiment 3
Take from the vinasse of Sichuan distillery, at first its component is measured.Measurement result shows that the water content of this vinasse is 63.5%, and the residual starch content of the vinasse of measuring with enzyme acid associating hydrolysis method is 9.8%, is 11.2% with the content of the crude protein of the vinasse of Kjeldahl determination mensuration, and the mass ratio of rice husk is 65%.
(1) gets the above-mentioned vinasse of 200kg,, spray into the yellow water of 50kg in the time of reinforced more uniformly at first with its steam-explosion jar that adds 0.5 cubic metre, be warmed up to 120 ℃, begin to reclaim the ester class, adopt GC7890-MS5973N type gas chromatograph-mass spectrometer, chromatographic condition: HP-INNOWAX chromatographic column (30m * 250 μ m * 0.25 μ m), adopt temperature programming: 45 ℃ of initial temperature, constant temperature 5min rises to 195 ℃ with 5 ℃ of per minutes, keeps 2min, be raised to 220 ℃ with 7 ℃ of per minutes, keep 8min to finish.220 ℃ of injector temperatures; Carrier gas is a high-pure helium, flow velocity 1.0ml/min; Sample size 1.0 μ L; Splitting ratio 10: 1.The mass spectrum condition: the ionization mode is electron-bombardment, electron energy 70eV, 220 ℃ of interface temperature, electron-multiplier voltage 1.89KV; Sweep limit 35-500u adopts the full scan mode of operation.According to the chromatogram assay, the rate of recovery of the fragrance matter in the vinasse is 70.5%.
(2) close water distilling apparatus, continue to heat up, arrive the quick-fried condition of vapour material is carried out the quick-fried processing of vapour, the quick-fried treatment condition of vapour are: the 0.6MPa dimension presses 50min to carry out the quick-fried processing of vapour.
(3) material after the quick-fried processing of vapour utilizes the continuous solid-state fermentation of 120L and the product gas-stripping heat-pump isolating equipment that is coupled that the residual starch in the vinasse is carried out the yeast ethanol fermentation, active dry yeast adds according to 1: 10000 the ratio of mass ratio of yeast and material, and saccharifying enzyme adds according to 1: 5000 the ratio of mass ratio of saccharifying enzyme and material; The ethanol that utilizes carbonic acid gas circulation gas to carry the fermentation generation in the time of fermentation proposes; Alcoholic acid output is 3.5% of vinasse after testing.
(4) its water content of material of coming out from fermentation reactor has been reduced to about 30%.Use vinasse mechanical classification device that it is carried out classification, this sorting equipment is to be transformed into after the orifice plate of YTJ-1 type corn threshing motor spindle is replaced with 14 purpose Stainless Steel Cloths.Utilize this device, the revolution of regulating grading wheel is 2000 commentariess on classics/min, can easily the most rice husks in the vinasse be separated, isolating effect be the rice husk part account for 48% of gross weight, rice husk rice husk ratio partly is up to 85%.Almost cannot see complete rice husk in the protein part and be rich in.
(5) vinasse separate obtain be rich in protein part in solid-state fermenter further microbe inoculation carry out aerobic fermentation and improve its proteic content.Concrete grammar is, the water content of regulating material is 60%, koning trichoderma and green wooden enzyme are carried out the two-stage liquid seed culture, seed liquor is inoculated according to 10% of material dry weight, put into solid-state fermenter after mixing thoroughly after the inoculation and carry out aerobic fermentation, fermenting process passes through the temperature of the Ventilation Control bed of material about 28-32 ℃, and fermentation time is 72h, cultivates material after the fermentation ends and covers with uniform mycelium.With cultured fermentation material 120 ℃ down dry, pulverize and promptly obtain crude protein content at 36.1% high protein feed.
(6) rice husk that classification is obtained partly pulverize the back replace the part perlite, through adding cement, regulating explained hereafter roofing board, floor plates such as water content, stirring, moulding.
Embodiment 4
Take from the vinasse of Sichuan distillery, at first its component is measured.Measurement result shows that the water content of this vinasse is 63.5%, and the residual starch content of the vinasse of measuring with enzyme acid associating hydrolysis method is 9.8%, is 11.2% with the content of the crude protein of the vinasse of Kjeldahl determination mensuration, and the mass ratio of rice husk is 65%.
(1) gets the above-mentioned vinasse of 200kg,, spray into the yellow water of 100kg in the time of reinforced more uniformly at first with its steam-explosion jar that adds 0.5 cubic metre, be warmed up to 180 ℃, begin to reclaim the ester class, adopt GC7890-MS5973N type gas chromatograph-mass spectrometer, chromatographic condition: HP-INNOWAX chromatographic column (30m * 250 μ m * 0.25 μ m), adopt temperature programming: 45 ℃ of initial temperature, constant temperature 5min rises to 195 ℃ with 5 ℃ of per minutes, keeps 2min, be raised to 220 ℃ with 7 ℃ of per minutes, keep 8min to finish.220 ℃ of injector temperatures; Carrier gas is a high-pure helium, flow velocity 1.0ml/min; Sample size 1.0 μ L; Splitting ratio 10: 1.The mass spectrum condition: the ionization mode is electron-bombardment, electron energy 70eV, 220 ℃ of interface temperature, electron-multiplier voltage 1.89KV; Sweep limit 35-500u adopts the full scan mode of operation.According to the chromatogram assay, the rate of recovery of the fragrance matter in the vinasse is 78.42%.
(2) close water distilling apparatus, continue to heat up, arrive the quick-fried condition of vapour material is carried out the quick-fried processing of vapour, the quick-fried treatment condition of vapour are: the 1.2MPa dimension presses 1min to carry out the quick-fried processing of vapour.
(3) material after the quick-fried processing of vapour utilizes the continuous solid-state fermentation of 120L and the product gas-stripping heat-pump isolating equipment that is coupled that the residual starch in the vinasse is carried out the yeast ethanol fermentation, active dry yeast adds according to 1: 5000 the ratio of mass ratio of yeast and material, and saccharifying enzyme adds according to 1: 2500 the ratio of mass ratio of saccharifying enzyme and material; The ethanol that utilizes carbonic acid gas circulation gas to carry the fermentation generation in the time of fermentation proposes; Alcoholic acid output is 3.8% of vinasse after testing.
(4) its water content of material of coming out from fermentation reactor has been reduced to about 30%.Use vinasse mechanical classification device that it is carried out classification, this sorting equipment is to be transformed into after the orifice plate of YTJ-1 type corn threshing motor spindle is replaced with 10 purpose Stainless Steel Cloths.Utilize this device, the revolution of regulating grading wheel is 2000 commentariess on classics/min, can easily the most rice husks in the vinasse be separated, isolating effect be the rice husk part account for 57% of gross weight, rice husk rice husk ratio partly is up to 85%.Almost cannot see complete rice husk in the protein part and be rich in.
(5) vinasse separate obtain be rich in protein part 120 ℃ dry down, pulverize and promptly obtain crude protein content at 29.4% high protein feed.
(6) rice husk that classification is obtained partly pulverize the back replace the part perlite, through adding cement, regulating explained hereafter wall body bricks such as water content, stirring, moulding.
Claims (9)
1. a distillers ' grains is put forward ester-fermentation-classification Poly-generation and is utilized method, may further comprise the steps:
(1) vinasse is packed in the quick-fried equipment of vapour of being with water distilling apparatus, spray into liquor fermentation by product yellow water or residual water, feed vapor distillation, reclaim the volatile matter in vinasse and the yellow water;
(2) close water distilling apparatus, continue to feed steam, carry out the quick-fried processing of vapour;
(3) access active dry yeast of the material after the quick-fried processing of vapour and saccharifying enzyme carry out solid state fermentation production ethanol, and the ethanol that utilizes carbonic acid gas circulation gas to carry the fermentation generation in the time of fermentation proposes;
(4) material that comes out from fermentation reactor utilizes task equipment to carry out mechanical classification, will be rich in proteic part and partly separate with rice husk;
(5) separating the direct or aerobic solid-state mixed fungus fermentation of protein part that is rich in that obtains further increases the protein content after drying, pulverizes the back as high protein feed;
(6) the rice husk part that separate to obtain directly or after the drying treatment as the raw material of making material of construction.
2. a kind of according to claim 1 distillers ' grains is put forward ester-fermentation-classification Poly-generation and is utilized method, the described feeding vapor distillation of step (1) wherein, and distillation temperature is 40-180 ℃.
3. a kind of according to claim 1 distillers ' grains is put forward ester-fermentation-classification Poly-generation and is utilized method, and wherein the described volatile matter of step (1) mainly is ethyl acetate, ethyl hexanoate, ethyl lactate, ethyl butyrate and ethanol.
4. a kind of according to claim 1 distillers ' grains is put forward ester-fermentation-classification Poly-generation and is utilized method, and wherein the condition of the quick-fried processing of the described vapour of step (2) is pressure 0.6-1.2MPa, dimension pressure time 1-50min.
5. a kind of according to claim 1 distillers ' grains is put forward ester-fermentation-classification Poly-generation and is utilized method, wherein the described active dry yeast of step (3) is according to the mass ratio 1 of yeast and material: 1000-1: 10000 ratio is added, and saccharifying enzyme is according to the mass ratio 1 of saccharifying enzyme and material: 1000-1: 5000 ratio interpolation.
6. a kind of according to claim 1 distillers ' grains is put forward ester-fermentation-classification Poly-generation and is utilized method, and wherein the described solid state fermentation of step (3) is batch solid state fermentation or continuous solid-state fermentation.
7. a kind of according to claim 1 distillers ' grains is put forward ester-fermentation-classification Poly-generation and is utilized method, and wherein the described stock grading specific equipment of step (4) is based on maize sheller, and the orifice plate of thresing machine bottom is replaced with 8 orders-14 purpose screen cloth.
8. a kind of distillers ' grains as claimed in claim 1 is put forward ester-fermentation-classification Poly-generation and is utilized method, and wherein the described mixed bacterium of step (5) is one or more in Penicillium decumbens, viride, koning trichoderma, whiterot fungi, geotrichum candidum, aspergillus niger, the Nocardia bacteria.
9. a kind of distillers ' grains as claimed in claim 1 is put forward ester-fermentation-classification Poly-generation and is utilized method, and wherein the described material of construction of step (6) is ash wall body plate, wall body brick, ecological plate, roof boarding, floor plates.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633400A (en) * | 2012-04-27 | 2012-08-15 | 庞全岭 | Method for treating bottom boiler water |
| CN102669490A (en) * | 2012-06-12 | 2012-09-19 | 宜宾学院 | Method for preparing fish feed by using white spirit waste spent grains or mixing white spirit waste spent grains with beer grains under the condition of photosynthetic bacteria fermentation |
| CN107008324A (en) * | 2017-03-15 | 2017-08-04 | 湖北大学 | A kind of vinasse load the preparation method and application of heterogeneous catalysis |
| CN109912318A (en) * | 2019-03-05 | 2019-06-21 | 沈阳化工大学 | A kind of spirit distiller grain sub-prime utilizes method |
| CN110438048A (en) * | 2019-08-23 | 2019-11-12 | 华中农业大学 | Utilize the method and its application of spirit stillage production Bacillus polymyxa additive |
| CN110452850A (en) * | 2019-08-23 | 2019-11-15 | 华中农业大学 | A kind of bacillus amyloliquefaciens culture, preparation method and applications |
| CN115074261A (en) * | 2022-06-28 | 2022-09-20 | 四川轻化工大学 | Saccharomyces boulardii, fermentation medium thereof, ruminant feed and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1160760A (en) * | 1996-11-15 | 1997-10-01 | 邢贯森 | Comprehensive utilization method of scented distillers' grains |
| CN1285156A (en) * | 1999-08-23 | 2001-02-28 | 中国环境科学研究院 | Technology for producing vinasse somatic protein fodder by solide aerobic fermentation |
| WO2008003692A1 (en) * | 2006-07-03 | 2008-01-10 | John Mahler | Method for the production of bioethanol and for the coproduction of energy from a starchy plant starting material |
| CN101768606A (en) * | 2010-02-24 | 2010-07-07 | 中国科学院过程工程研究所 | Method for solid state fermentation of ethanol through steam explosion of cassava and comprehensive utilization of cassava |
-
2010
- 2010-11-04 CN CN2010105317537A patent/CN102021204B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1160760A (en) * | 1996-11-15 | 1997-10-01 | 邢贯森 | Comprehensive utilization method of scented distillers' grains |
| CN1285156A (en) * | 1999-08-23 | 2001-02-28 | 中国环境科学研究院 | Technology for producing vinasse somatic protein fodder by solide aerobic fermentation |
| WO2008003692A1 (en) * | 2006-07-03 | 2008-01-10 | John Mahler | Method for the production of bioethanol and for the coproduction of energy from a starchy plant starting material |
| CN101768606A (en) * | 2010-02-24 | 2010-07-07 | 中国科学院过程工程研究所 | Method for solid state fermentation of ethanol through steam explosion of cassava and comprehensive utilization of cassava |
Non-Patent Citations (3)
| Title |
|---|
| 《环境科学学报》 20000930 李政一 等 酒糟资源化研究 第20卷, * |
| 《酿酒》 19961231 周恒刚，张志民 酒糟的利用（二）白酒糟的场内消化 , 第3期 * |
| 《食品科学》 20080815 吴忠会 等 白酒丢糟"零排放"的研究 第29卷, 第8期 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633400A (en) * | 2012-04-27 | 2012-08-15 | 庞全岭 | Method for treating bottom boiler water |
| CN102669490A (en) * | 2012-06-12 | 2012-09-19 | 宜宾学院 | Method for preparing fish feed by using white spirit waste spent grains or mixing white spirit waste spent grains with beer grains under the condition of photosynthetic bacteria fermentation |
| CN107008324A (en) * | 2017-03-15 | 2017-08-04 | 湖北大学 | A kind of vinasse load the preparation method and application of heterogeneous catalysis |
| CN109912318A (en) * | 2019-03-05 | 2019-06-21 | 沈阳化工大学 | A kind of spirit distiller grain sub-prime utilizes method |
| CN110438048A (en) * | 2019-08-23 | 2019-11-12 | 华中农业大学 | Utilize the method and its application of spirit stillage production Bacillus polymyxa additive |
| CN110452850A (en) * | 2019-08-23 | 2019-11-15 | 华中农业大学 | A kind of bacillus amyloliquefaciens culture, preparation method and applications |
| CN115074261A (en) * | 2022-06-28 | 2022-09-20 | 四川轻化工大学 | Saccharomyces boulardii, fermentation medium thereof, ruminant feed and preparation method thereof |
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