CN101979627A - Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli - Google Patents
Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli Download PDFInfo
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- CN101979627A CN101979627A CN2010102991142A CN201010299114A CN101979627A CN 101979627 A CN101979627 A CN 101979627A CN 2010102991142 A CN2010102991142 A CN 2010102991142A CN 201010299114 A CN201010299114 A CN 201010299114A CN 101979627 A CN101979627 A CN 101979627A
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Abstract
The invention relates to a method for preparing a glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli, which solves the problem that environment can be polluted by directly discharging fermented waste thalli or economic benefit is low when the fermented waste thalli are prepared into a feed additive in the conventional glutamic acid production. The glutamic acid fermentation waste thalli are taken as raw materials; protein hydrolyzate is obtained by enzymatic hydrolysis, acid hydrolysis or an enzymatic hydrolysis and acid hydrolysis combined method; and the hydrolyzate is concentrated or dried to prepare the organic nitrogen additive for fermentation. For the organic nitrogen additive prepared by the method, the protein recovery rate of the glutamic acid fermentation waste thalli reaches 93 percent; the prepared organic nitrogen additive can be recycled for glutamic acid fermentation and replaces yeast extract, peptone, corn steep liquor and other organic nitrogen additives which are used in the conventional glutamic acid production, so environmental pollution can be reduced and the added value of the organic nitrogen additive can be greatly improved; and the organic nitrogen additive has great significance for the comprehensive utilization of the glutamic acid fermentation waste thalli.
Description
[technical field]
The invention belongs to the comprehensive utilization technique field of gourmet powder fermenting industry, be specifically related to a kind of method of utilizing the glutamic acid fermentation waste thallus to prepare glutamic acid fermentation organonitrogen additive.
[technical background]
China is glutamate production big country, the a large amount of monosodium glutamate of annual production, 1 ton of monosodium glutamate of general production will produce the organic waste water of 20～25 tons of high densitys, contain 2%～5% glutamic acid fermentation waste thallus in the waste liquid, nutritive substance such as rich in proteins, nucleic acid wherein, directly the discharging meeting pollutes environment, and dry back is also lower as the fodder additives economic benefit.
[summary of the invention]
The objective of the invention is to solve the organic waste water that produced in the glutamate production process directly the discharging meeting environment is polluted or directly utilizes the lower problem of its economic worth, providing a kind of is the method for feedstock production glutamic acid fermentation organonitrogen additive with the glutamic acid fermentation waste thallus.
The method of utilizing the glutamic acid fermentation waste thallus to prepare glutamic acid fermentation organonitrogen additive (a kind of organonitrogen additive alpha-amino nitrogen that is rich in) provided by the invention, be to be raw material with the glutamic acid fermentation waste thallus, adopt enzymatic hydrolysis, acid system hydrolysis or enzyme process to obtain glutamic acid fermentation organonitrogen additive in conjunction with the acid system hydrolysis, the concrete steps of this method are:
1st, be raw material with the glutamic acid fermentation waste thallus, after washing, the allotment cell concentration is 10-20g/100mL;
2nd, the thalline that the 1st step is joined carries out the high-pressure homogenization processing, and condition is 70MPa homogenate 6 times;
3rd, the 2nd suspension of step behind high-pressure homogenization is carried out enzyme process, acid system or enzyme process in conjunction with the acid system hydrolysis, hydrolysising condition is respectively:
The enzymatic hydrolysis condition is: pH 4.0, and enzymolysis time is 10 hours, and the temperature of enzymolysis is 50 ℃, and aspartic protease and dextranase compositely proportional are 3: 1, and adding total amount is the 6%-8% (W) of thalline quality;
The acid system hydrolysising condition is: pH 0.5, and hydrolysis temperature is 115 ℃, and hydrolysis time is 18 hours, and used mineral acid is a hydrochloric acid;
Enzyme process in conjunction with the condition of acid system hydrolysis is: pH 4.0, add proteolytic enzyme and dextranase prozyme according to thalline quality 2%, and compositely proportional is 3: 1, and 50 ℃, the hydrolysis enzyme that goes out after 10 hours, centrifugation is residue obtained in 115 ℃, 0.5 time acid hydrolysis of pH 18 hours;
4th, with the product behind the 3rd one-step hydrolysis through centrifugal or filter, can obtain described glutamic acid fermentation organonitrogen additive.Hydrolysate further concentrates or spraying drying can obtain being used to promote the substratum organonitrogen additive of microorganism growth.
Utilize this method to produce glutamic acid fermentation organonitrogen additive, the protein recovery of glutamic acid fermentation waste thallus is 70%-93%, and glutamic acid fermentation waste thallus protein recovery can reach 93% when particularly utilizing enzyme acid combined techniques.
Advantage of the present invention and positively effect
The invention provides a kind of is raw material with the glutamic acid fermentation waste thallus, the method for preparing the organonitrogen additive of corynebacterium glutamicum fermentation through hydrolysis, use the protein recovery of this method glutamic acid fermentation waste thallus to be 80%-90%, it is concentrated or drying can make fermentation and uses the organonitrogen additive, be used for replacing organonitrogen additives such as present glutamic acid fermentation process yeast extract paste, peptone, corn steep liquor.Yet there are no and prepare the organonitrogen additive with the glutamic acid fermentation waste thallus and be used for fermenting process, the thalline of the present invention after for glutamic acid fermentation provides new market outlook to use widely.
The inventive method has not only solved problem of environmental pollution, the more important thing is and can directly improve economic benefit of enterprises, compares with making fodder additives, and its value is higher, and income is also better, and the organic liquid waste in the glutamate production is turned waste into wealth.
[embodiment]
Embodiment 1 enzymolysis process
(1) the prozyme proportioning is to the proteoclastic influence of glutamic acid fermentation waste thallus
Corynebacterium glutamicum is made into the bacterium liquid of 20g/100mL, and through the high-pressure homogenization pre-treatment, the homogenate condition: homogenate is 6 times under the 70MPa.Add the proteolytic enzyme and the dextranase of different ratios, the enzyme addition is 1%, 50 ℃ of temperature, and its tropina rate of recovery, result such as table 1 are surveyed in hydrolysis after 10 hours.As seen from Table 1, when proteolytic enzyme and dextranase compositely proportional were 3: 1, glutamic acid fermentation waste thallus protein recovery was higher, and aspartic protease and dextranase composite thallus protein recovery are up to: 64.27%.
Glutamic acid fermentation waste thallus protein recovery (%) under the different adding proportions of table 1 prozyme
(2) the enzyme addition is to the influence of glutamic acid fermentation waste thallus protein recovery
Corynebacterium glutamicum liquid is made into the bacterium liquid of 20g/100mL, and through the high-pressure homogenization pre-treatment, the homogenate condition: homogenate is 6 times under the 70MPa.By proteolytic enzyme and dextranase compositely proportional is 3: 1, add 2%, 4%, 6%, 8% proteolytic enzyme and dextranase respectively, 50 ℃ of temperature, the tropina rate of recovery was surveyed in hydrolysis in 10 hours, result such as table 2, along with the increase of enzyme concentration, protein recovery increases gradually as seen from Table 2, and aspartic protease is better than other enzyme combination with the combination of dextranase.And enzyme concentration be 8% o'clock of the thalline quality be 2% o'clock of thalline quality than enzyme concentration, glutamic acid fermentation waste thallus protein recovery improves 5-10%.
Glutamic acid fermentation waste thallus protein recovery (%) under the different additions of table 2
We obtain working as aspartic protease and the dextranase compositely proportional is 3: 1, and glutamic acid fermentation waste thallus protein recovery can reach more than 70% when addition was 6%-8%.
Embodiment 2: acid hydrolyzation
(1) pH is to the influence of the tropina rate of recovery
Corynebacterium glutamicum is made into the bacterium liquid of 5g/100mL, transfers pH to 0.5,1.5,2.5,3.5 respectively with 6mol/L HCl, 115 ℃ of following hydrolysis 20 hours.The results are shown in Table 3, as seen from Table 3, pH is very big to acid-hydrolyzed influence, and glutamic acid fermentation waste thallus protein recovery high nearly 60% when the protein recovery when pH 0.5 was 3.5 than pH.
Glutamic acid fermentation waste thallus protein recovery (%) under the different pH of table 3
(2) cell concentration is to acid-hydrolyzed influence
The bacterium liquid 50ml that corynebacterium glutamicum is made into 5g/100mL, 10g/100mL, 15g/100mL, 20g/100mL respectively puts into the 250ml triangular flask, add 6mol/LHCl and transfer pH 0.5,115 ℃ of following hydrolysis 18 hours, the tropina rate of recovery sees Table 4, by table 4 as seen, raising along with cell concentration, protein recovery reduces gradually, when cell concentration is when being elevated to 10% by 5%, the tropina protein recovery descends more obvious, and the variation of the tropina rate of recovery is not very greatly when cell concentration is raised to 20% by 10%.
Glutamic acid fermentation waste thallus protein recovery (%) under the different cell concentrations of table 4
Embodiment 3: enzyme acid combined techniques
(1) the Corynebacterium glutamicum body is allocated the corynebacterium glutamicum liquid of concentration 20g/100mL after washing;
(2) adopt high pressure homogenizer that Corynebacterium glutamicum liquid is carried out homogenized, homogenate is 6 times under the 70MPa condition;
(3) at 50 ℃, adopt sodium hydroxide solution to transfer pH 4.0, add proteolytic enzyme and dextranase prozyme (adding proportion 3: 1, addition 2%), the hydrolysis enzyme that goes out after 10 hours, centrifugal, then its protein recovery is 65.31%;
(4) with the residue behind the enzymolysis in 115 ℃, 0.5 time acid hydrolysis of pH, hydrolysis is after 18 hours, and is centrifugal, surveying its protein recovery is 80.02%;
(5) enzyme acid combined techniques is handled, and the bacterial protein rate of recovery is 93%.
Embodiment 4 different nitrogen sources are cultivated glutamic acid fermentation seed effect relatively
In basic medium, add urea, corn steep liquor, yeast respectively and soak powder and the obtained organonitrogen additive of present method, its nitrogen content is 0.4g/100mL, putting into liquid amount respectively is the 250ml triangular flask of 50ml, cultivated 10 hours in 28 ℃ of 160rpm, use the spectrophotometry cell concn, it the results are shown in Table 5.
Table 5 different nitrogen sources hypothallus OD value
In seed culture, make its cell concentration of cultivating of nitrogenous source as can be seen from Table 5 and be higher than urea and corn with the organonitrogen additive of glutamic acid fermentation waste thallus preparation, to soak powder similar with yeast, can substitute corn steep liquor, peptone or yeast and soak powder and be used for L-glutamic acid production in industrial production.
Claims (4)
1. method of utilizing the glutamic acid fermentation waste thallus to prepare glutamic acid fermentation organonitrogen additive, it is characterized in that with the glutamic acid fermentation waste thallus be raw material, adopt enzymatic hydrolysis, acid system hydrolysis or enzyme process to obtain glutamic acid fermentation organonitrogen additive in conjunction with the acid system hydrolysis.
2. method according to claim 1 is characterized in that described enzymatic hydrolysis, and Corynebacterium glutamicum is made into the bacterium liquid that concentration is 20g/100mL, and high-pressure homogenization is 6 times under the 70MPa; With proteolytic enzyme and dextranase coordinated enzymatic hydrolysis, enzymolysis is 10 hours under pH4.0, and the temperature of enzymolysis is 50 ℃, and aspartic protease and dextranase compositely proportional are 3: 1, the enzyme addition is 6% o'clock of thalline quality, and glutamic acid fermentation waste thallus protein recovery can reach 70%.
3. method according to claim 1, it is characterized in that described acid system hydrolysis, wherein used mineral acid is a hydrochloric acid, the acid hydrolysis condition is: pH 0.5, cell concentration is 20g/100ml, hydrolysis temperature is 115 ℃, and hydrolysis time is 18 hours, and glutamic acid fermentation waste thallus protein recovery is 70%.
4. method according to claim 1, it is characterized in that enzyme process in conjunction with the condition of acid system hydrolysis is: Corynebacterium glutamicum is made into the bacterium liquid that concentration is 20g/100mL, high-pressure homogenization is 6 times under the 70MPa, transfer pH 4.0, add proteolytic enzyme and dextranase prozyme according to thalline quality 2%, the adding proportion of proteolytic enzyme and dextranase is 3: 1,50 ℃, the hydrolysis enzyme that goes out after 10 hours, centrifugation is residue obtained in 115 ℃, 0.5 time acid hydrolysis of pH 18 hours, glutamic acid fermentation waste thallus albumen total yield 93%.
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| CN102701507A (en) * | 2012-06-26 | 2012-10-03 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for treating high-concentration wastewater of glutamic acid |
| CN102719510A (en) * | 2012-06-26 | 2012-10-10 | 呼伦贝尔东北阜丰生物科技有限公司 | Amino acid fermentation bacteria utilization method |
| CN102732589A (en) * | 2012-06-26 | 2012-10-17 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for treating threonine mother liquor |
| CN105936924A (en) * | 2016-07-16 | 2016-09-14 | 内蒙古阜丰生物科技有限公司 | New process for preparing polyglutamic acid from crop straw |
| CN105950676A (en) * | 2016-07-16 | 2016-09-21 | 内蒙古阜丰生物科技有限公司 | Technology for preparing, separating and purifying polyglutamic acid |
| CN105950677A (en) * | 2016-07-22 | 2016-09-21 | 内蒙古阜丰生物科技有限公司 | Technology for hydrolyzing waste glutamic acid fermentation thalli for fermentation |
| CN105950678A (en) * | 2016-07-22 | 2016-09-21 | 内蒙古阜丰生物科技有限公司 | Method for preparing fermentation culture medium from glutamic acid fermentation waste bacteria |
| CN106191180A (en) * | 2016-07-15 | 2016-12-07 | 内蒙古阜丰生物科技有限公司 | Fermented abandoned thalline and agricultural wastes are utilized to combine the method preparing polyglutamic acid |
| CN106191144A (en) * | 2016-07-15 | 2016-12-07 | 内蒙古阜丰生物科技有限公司 | A kind of new technology utilizing glutamic acid production waste material to prepare polyglutamic acid |
| CN107164247A (en) * | 2017-06-03 | 2017-09-15 | 秦皇岛华恒生物工程有限公司 | A kind of method of utilization L alanine fermentation wastes culture yeasts |
| CN107549760A (en) * | 2017-09-19 | 2018-01-09 | 广东肇庆星湖生物科技股份有限公司 | A kind of preparation method of compound nutritional flavoring agent |
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| CN110777172A (en) * | 2019-09-12 | 2020-02-11 | 赵兰坤 | Enzymolysis process of amino acid fermentation thallus |
| CN110846352A (en) * | 2019-09-11 | 2020-02-28 | 赵兰坤 | Preparation method of glutamic acid fermentation medium |
| WO2020140388A1 (en) * | 2019-01-02 | 2020-07-09 | 呼伦贝尔东北阜丰生物科技有限公司 | Glutamic acid green clean fermentation process |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1235780A (en) * | 1998-05-19 | 1999-11-24 | 菱花集团公司 | Method for preparing composite seasoning juice by using glutamic acid thallus |
| CN1733925A (en) * | 2004-08-12 | 2006-02-15 | 甘肃省科学院生物研究所 | Method with discarded protein Preparation Moriamin S |
| CN101401589A (en) * | 2008-11-19 | 2009-04-08 | 东北农业大学 | Method of preparing microbial source stress resistance |
| CN101457242A (en) * | 2009-01-06 | 2009-06-17 | 天津科技大学 | Novel process for greenly and high efficiently improving L-glutamic acid fermentation production rate |
-
2010
- 2010-10-08 CN CN2010102991142A patent/CN101979627A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1235780A (en) * | 1998-05-19 | 1999-11-24 | 菱花集团公司 | Method for preparing composite seasoning juice by using glutamic acid thallus |
| CN1733925A (en) * | 2004-08-12 | 2006-02-15 | 甘肃省科学院生物研究所 | Method with discarded protein Preparation Moriamin S |
| CN101401589A (en) * | 2008-11-19 | 2009-04-08 | 东北农业大学 | Method of preparing microbial source stress resistance |
| CN101457242A (en) * | 2009-01-06 | 2009-06-17 | 天津科技大学 | Novel process for greenly and high efficiently improving L-glutamic acid fermentation production rate |
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| CN102701507A (en) * | 2012-06-26 | 2012-10-03 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for treating high-concentration wastewater of glutamic acid |
| CN102719510A (en) * | 2012-06-26 | 2012-10-10 | 呼伦贝尔东北阜丰生物科技有限公司 | Amino acid fermentation bacteria utilization method |
| CN102732589A (en) * | 2012-06-26 | 2012-10-17 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for treating threonine mother liquor |
| CN102732589B (en) * | 2012-06-26 | 2014-03-12 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for treating threonine mother liquor |
| CN106191144A (en) * | 2016-07-15 | 2016-12-07 | 内蒙古阜丰生物科技有限公司 | A kind of new technology utilizing glutamic acid production waste material to prepare polyglutamic acid |
| CN106191180A (en) * | 2016-07-15 | 2016-12-07 | 内蒙古阜丰生物科技有限公司 | Fermented abandoned thalline and agricultural wastes are utilized to combine the method preparing polyglutamic acid |
| CN106191180B (en) * | 2016-07-15 | 2021-01-05 | 内蒙古阜丰生物科技有限公司 | Method for jointly preparing polyglutamic acid by utilizing fermentation waste thalli and agricultural wastes |
| CN106191144B (en) * | 2016-07-15 | 2021-01-05 | 内蒙古阜丰生物科技有限公司 | Novel process for preparing polyglutamic acid by utilizing glutamic acid production waste |
| CN105950676A (en) * | 2016-07-16 | 2016-09-21 | 内蒙古阜丰生物科技有限公司 | Technology for preparing, separating and purifying polyglutamic acid |
| CN105936924B (en) * | 2016-07-16 | 2021-01-01 | 内蒙古阜丰生物科技有限公司 | Novel process for preparing polyglutamic acid by using crop straws |
| CN105936924A (en) * | 2016-07-16 | 2016-09-14 | 内蒙古阜丰生物科技有限公司 | New process for preparing polyglutamic acid from crop straw |
| CN105950676B (en) * | 2016-07-16 | 2021-01-05 | 内蒙古阜丰生物科技有限公司 | Process for preparing, separating and purifying polyglutamic acid |
| CN105950677A (en) * | 2016-07-22 | 2016-09-21 | 内蒙古阜丰生物科技有限公司 | Technology for hydrolyzing waste glutamic acid fermentation thalli for fermentation |
| CN105950678A (en) * | 2016-07-22 | 2016-09-21 | 内蒙古阜丰生物科技有限公司 | Method for preparing fermentation culture medium from glutamic acid fermentation waste bacteria |
| CN107164247A (en) * | 2017-06-03 | 2017-09-15 | 秦皇岛华恒生物工程有限公司 | A kind of method of utilization L alanine fermentation wastes culture yeasts |
| CN107549760A (en) * | 2017-09-19 | 2018-01-09 | 广东肇庆星湖生物科技股份有限公司 | A kind of preparation method of compound nutritional flavoring agent |
| CN109609567A (en) * | 2018-12-30 | 2019-04-12 | 新疆阜丰生物科技有限公司 | A kind of L-Trp Green production method replacing yeast powder using mycoprotein enzymolysis liquid |
| CN109609567B (en) * | 2018-12-30 | 2022-05-03 | 新疆阜丰生物科技有限公司 | Green production method of L-tryptophan by using mycoprotein enzymolysis liquid to replace yeast powder |
| WO2020140388A1 (en) * | 2019-01-02 | 2020-07-09 | 呼伦贝尔东北阜丰生物科技有限公司 | Glutamic acid green clean fermentation process |
| CN110846352A (en) * | 2019-09-11 | 2020-02-28 | 赵兰坤 | Preparation method of glutamic acid fermentation medium |
| CN110777172A (en) * | 2019-09-12 | 2020-02-11 | 赵兰坤 | Enzymolysis process of amino acid fermentation thallus |
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Application publication date: 20110223 |