CN101919435B - Sashimi preservative containing active probiotics as well as preparation method and application thereof - Google Patents
Sashimi preservative containing active probiotics as well as preparation method and application thereof Download PDFInfo
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- CN101919435B CN101919435B CN2010102493999A CN201010249399A CN101919435B CN 101919435 B CN101919435 B CN 101919435B CN 2010102493999 A CN2010102493999 A CN 2010102493999A CN 201010249399 A CN201010249399 A CN 201010249399A CN 101919435 B CN101919435 B CN 101919435B
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Abstract
The invention discloses a sashimi preservative containing active probiotics as well as a preparation method and application thereof. The sashimi preservative comprises the following components: 2-8% of fish viscera, 0.3-0.5% of K3PO4.3H2O, 0.3-0.8% of CH3COONa, 3-5% of glucose and 1-8% of probiotics solution. The preparation method comprises the following steps of: firstly, uniformly mixing the fish viscera, K3PO4.3H2O, CH3COONa, glucose and water, and sterilizing; then, adding the probiotics solution in the logarithmic growth phase, and fermenting to obtain the sashimi preservative containing the active probiotics. The preparation method of the sashimi preservative is simple, the comprehensive utilization value of the fish viscera is increased by using the fish viscera as a raw material to prepare the preservative, and the prepared sashimi preservative is safe and low in toxicity and combines preservation effect and health care function.
Description
Technical field
The present invention relates to a kind of food preservative and preparation method thereof, particularly a kind of raw fish anticorrisive agent that contains active probiotic and preparation method thereof and application belong to biological food preservative technology field.
Background technology
Also known as sashimi sashimi, ancient fish kuai, kuai or grouper, fresh fish is sliced raw shellfish, dip seasoning food consumed collectively.Raw fish originates from China, and long history is arranged, after reach ground such as Japan, the Korea peninsula, be the food that is popular in Japan.Raw fish is made simple, and is edible good to eat, nutritious; But also must weigh its advantages against its disadvantages simultaneously: say from the nutrition angle from positive and negative two aspects; Through cooking process such as not traditional stir-fry, fried, steamings, nutriment keeps better raw fish, is the dish that is rich in nutrition together; But the shelf-life of raw fish is too short, all is basically to cut at present to eat at present, can not be stored for a long time.
Do not see also at present that the instant food that raw fish is arranged occurs, its main cause is that the shelf life of raw fish is too short.And the research of relevant raw fish anticorrisive agent product does not also see that report is arranged.
Summary of the invention
The shortcoming that primary and foremost purpose of the present invention is to overcome prior art provides a kind of raw fish anticorrisive agent that contains active probiotic with not enough.
Another object of the present invention is to provide the said preparation method who contains the raw fish anticorrisive agent of active probiotic.
A goal of the invention more of the present invention is to provide the said application that contains the raw fish anticorrisive agent of active probiotic.
Goal of the invention of the present invention realizes through following technical proposals: a kind of raw fish anticorrisive agent that contains active probiotic, contain following composition by mass percentage:
Fish guts 2～8%
K
3PO
4·3H
2O 0.3～0.5％
CH
3COONa 0.3～0.8％
Glucose 3～5%
Probio solution 1～8%
Surplus is a water.
Described fish guts is meant the mixture of the various internal organs except that the fish courage in the fish belly chamber;
The preferred fresh-water fishes of described fish; At least a in preferred especially Tilapia mossambica, perch, dace, grass carp or the silver carp;
Described probio comprises lactobacillus (Lactobacillus), Bifidobacterium (Bifidobacterium) and streptococcus thermophilus (Streptococcus thermophils) etc.; Be preferably lactobacillus, Bifidobacterium and streptococcus thermophilus and mix the mixture that forms; More preferably lactobacillus, Bifidobacterium and streptococcus thermophilus are not got rid of other probio and combination thereof in the mixture that bacterial population number ratio forms at 1: 1: 1;
The concentration of described probio solution is preferably 10
8CFU/ml.
The said preparation method who contains the raw fish anticorrisive agent of active probiotic comprises following steps:
(1) pre-treatment of fish guts: the fish guts behind the fish courage is removed in collection, cleans up, and draining rubs, and is centrifugal, removes the upper strata grease;
(2) preparation of probio solution: with the probio activation, be cultured to exponential phase, centrifugal, get deposition; Suspend with SPSS then and precipitate, obtain probio solution;
(3) contain the preparation of the raw fish anticorrisive agent of active probiotic: get the fish guts of step (1) preparation of mass percent 2～8%, the K of mass percent 0.3～0.5%
3PO
43H
2The CH of O, mass percent 0.3～0.8%
3The glucose and the water of COONa, mass percent 3～5% evenly mix, sterilization; Then be cooled to below 40 ℃, the probio solution of the step of cut-in quality percentage 1～8% (2) preparation ferments, and obtains containing the raw fish anticorrisive agent of active probiotic; Wherein, fish guts, K
3PO
43H
2O, CH
3The summation of COONa, glucose, probio solution and water is a mass percent 100%;
Preferred 0.5～1 hour of the time of draining described in the step (1);
The condition of rubbing described in the step (1) is preferably rubs 0.5～1 hour in meat grinder;
Centrifugal condition described in the step (1) is preferably centrifugal 5～10min in 2000～5000rpm;
The culture medium of cultivating described in the step (2) is preferably the MRS fluid nutrient medium;
Centrifugal condition described in the step (2) is preferably centrifugal 5～10min in 4000～8000rpm;
The concentration of probio solution is preferably 10 described in the step (2)
8CFU/ml;
Water described in the step (3) is preferably deionized water or distilled water;
The condition of sterilization is preferably in 121 ℃ of 15min at least that sterilize described in the step (3);
The condition of fermentation described in the step (3) is preferably in 30～37 ℃ of fermentations 2 days.
The said raw fish anticorrisive agent that contains active probiotic in the cold chain storing for well; Can according to the method for using difference select for use canned, flexible plastic is packed or bottled.Storage is after 6 months down in 4 ℃ for this product, and with the cell count of MRS plating medium, its bacterium number is kept better, and the bacterium number when encapsulating with product is suitable.
The said method for using that contains the raw fish anticorrisive agent of active probiotic: this anticorrisive agent is sprayed on the raw fish surface, and consumption is that every 50g raw fish sprays the said raw fish anticorrisive agent that contains active probiotic of 0.2～1ml.
The antibacterial principle of raw fish anticorrisive agent according to the invention is: fish guts contains the multiple nutritional labeling that supplies probio to utilize such as protein, unrighted acid, vitamin and mineral matter.Fish guts and probio together are sprayed at then to be induced on the flesh of fish and has promoted the utilize ability of probio to fish protein, thus make probio can be on raw fish growth and breeding.The getable nutriment of pathogenic bacteria has been competed in the existence of probio, and simultaneously probio is through producing materials such as acid, hydrogen peroxide, bacteriocin, removes or has reduced the adhesion of pathogenic bacteria on fillet, and suppressed the growth of pathogenic bacteria with this.The present invention makes full use of probio to this inhibitory action of flesh of fish spoilage organisms and the product processed, thereby has played the effect of anticorrisive agent.
The present invention has following advantage and effect:
1, traditionally, fish guts seldom obtains the utilization of high added value owing to contain poisonous heavy metal.Yet research shows that the fermentation raw material content of beary metal of (referring to fish guts in the present invention) can removed or reduce to probio; Add the addition of this anticorrisive agent when using seldom (every 50g raw fish only needs 0.2～1ml anticorrisive agent); Make that this anticorrisive agent toxicity when guaranteeing antiseptic effect is extremely low, adopting fish guts is the comprehensive utilization value that the feedstock production anticorrisive agent has also improved fish guts.
2, probio has certain health care, and the raw fish anticorrisive agent that has added probio also makes product (raw fish) have certain function of health care when playing antisepsis.
3, the preparation method of raw fish anticorrisive agent according to the invention is simple, and suitable fish packing house becomes to produce voluntarily to be used.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
(1) pre-treatment of fish guts: collect tilapia internal organs, remove the fish courage.Cleaning and removing is removed silt, and draining 0.5 hour rubbed 0.5 hour with meat grinder, and the centrifugal 8min of 3000rpm removes the upper strata grease.
(2) preparation of probio solution: cultivate bifidobacterium longum (Bifidobacterium lactis respectively with the cultivation of MRS fluid nutrient medium; Bi07) (available from Danisco A/S BJ Rep Office), lactobacillus fermenti (Lactobacillus fermentum; ATCC 9338) (available from Chinese industrial microorganism fungus kind preservation center) and streptococcus thermophilus (Streptococcus thermophils; CICC 6038) (available from Chinese industrial microorganism fungus kind preservation center) to logarithmic phase; Respectively at the centrifugal 8min of 5000rpm, get deposition, the concentration of using SPSS to adjust bacterium respectively again is 10
8Cfu/ml mixes than 1: 1: 1 according to liquor capacity then, obtains probio solution.
(3) contain the preparation of the raw fish anticorrisive agent of active probiotic: get the tilapia internal organs of step (1) preparation of mass percent 2%, the K of mass percent 0.5%
3PO
43H
2The CH of O, mass percent 0.3%
3The distilled water of the glucose of COONa, mass percent 5% and mass percent 91.2% mixes; In 121 ℃ of sterilization 15min, be cooled to below 40 ℃ the probio solution of the step of cut-in quality percentage 1% (2) preparation; In 37 ℃ of fermentations 2 days, obtain containing the anticorrisive agent of active probiotic.
(4) detect: cultivate counting with MRS culture medium flat plate anaerobism, wherein the bacterium viable count is no less than 10
8Cfu/ml.The storing of product cold chain.Storage is after 6 months down in 4 ℃, and with the dull and stereotyped anaerobic culture medium cell count of MRS, its bacterium number still reaches 10
8Cfu/ml.
(5) raw fish is dried by the fire to water content 40%～60% with low temperature, then foregoing preservatives is sprayed on the raw fish surface, consumption is 0.2～1ml/50g fillet, the nitrogen-filled packaging sealing.Transported and preserve 6 months under 4 ℃, flesh of fish common pathogen is detected, do not exceed standard.The mouthfeel of the flesh of fish does not normally have stale flavor.And do not use the raw fish pathogenic bacteria of anticorrisive agent to exceed standard in a large number, and has the stale flavor of the flesh of fish.
The anticorrisive agent Nantural non-toxic that contains active probiotic of present embodiment preparation, even can directly eat.Be used for the anticorrosion of raw fish, can make its shelf life reach 6 months.
Embodiment 2
(1) pre-treatment of fish guts: collect the perch internal organ, remove the fish courage.Cleaning and removing is removed silt, and draining 0.8 hour rubbed 0.8 hour with meat grinder, and the centrifugal 5min of 5000rpm removes the upper strata grease.
(2) preparation of probio solution: cultivate bifidobacterium longum (Bifidobacterium lactis respectively with the cultivation of MRS fluid nutrient medium; Bi07) (available from Danisco A/S BJ Rep Office), lactobacillus fermenti (Lactobacillus fermentum; ATCC 9338) (available from Chinese industrial microorganism fungus kind preservation center) and streptococcus thermophilus (Streptococcus thermophils; CICC 6038) (available from Chinese industrial microorganism fungus kind preservation center) to logarithmic phase; Respectively at the centrifugal 10min of 4000rpm, get deposition, the concentration of using SPSS to adjust bacterium respectively again is 10
8Cfu/ml mixes than 1: 1: 1 according to liquor capacity then, obtains probio solution.
(3) contain the preparation of the raw fish anticorrisive agent of active probiotic: get the perch internal organ of step (1) preparation of mass percent 4%, the K of mass percent 0.3%
3PO
43H
2The CH of O, mass percent 0.6%
3The distilled water of the glucose of COONa, mass percent 4% and mass percent 88.1% mixes; 121 ℃ of sterilization 15min are cooled to below 40 ℃, the probio solution of the step of cut-in quality percentage 3% (2) preparation; In 30 ℃ of fermentations 2 days, obtain containing the anticorrisive agent of active probiotic.
(4) detect: cultivate counting with MRS culture medium flat plate anaerobism, wherein the bacterium viable count is no less than 10
8Cfu/ml.The storing of product cold chain.Storage is after 6 months down in 4 ℃, and with the dull and stereotyped anaerobic culture medium cell count of MRS, its bacterium number still reaches 10
8Cfu/ml.
(5) raw fish is dried by the fire to water content 40%～60% with low temperature, then foregoing preservatives is sprayed on the raw fish surface, consumption is 0.2～1ml/50g fillet, the nitrogen-filled packaging sealing.Transported and preserve 6 months under 4 ℃, flesh of fish common pathogen is detected, do not exceed standard.The mouthfeel of the flesh of fish does not normally have stale flavor.And do not use the raw fish pathogenic bacteria of anticorrisive agent to exceed standard in a large number, and has the stale flavor of the flesh of fish.
The anticorrisive agent Nantural non-toxic that contains active probiotic of present embodiment preparation, even can directly eat.Be used for the anticorrosion of raw fish, can make its shelf life reach 6 months.
Embodiment 3
(1) pre-treatment of fish guts: collect the dace internal organ, remove the fish courage.Cleaning and removing is removed silt, and draining 0.5 hour rubbed 0.5 hour with meat grinder, and the centrifugal 5min of 4000rpm removes the upper strata grease.
(2) preparation of probio solution: cultivate bifidobacterium longum (Bifidobacterium lactis respectively with the cultivation of MRS fluid nutrient medium; Bi07) (available from Danisco A/S BJ Rep Office), lactobacillus fermenti (Lactobacillus fermentum; ATCC 9338) (available from Chinese industrial microorganism fungus kind preservation center) and streptococcus thermophilus (Streptococcus thermophils; CICC 6038) (available from Chinese industrial microorganism fungus kind preservation center) to logarithmic phase; Respectively at the centrifugal 5min of 8000rpm, get deposition, the concentration of using SPSS to adjust bacterium respectively again is 10
8Cfu/ml mixes than 1: 1: 1 according to liquor capacity then, obtains probio solution.
(3) contain the preparation of the raw fish anticorrisive agent of active probiotic: get the dace internal organ of step (1) preparation of mass percent 6%, the K of mass percent 0.3%
3PO
43H
2The CH of O, mass percent 0.8%
3The distilled water of the glucose of COONa, mass percent 5% and mass percent 86.9% mixes; 121 ℃ of sterilization 15min are cooled to below 40 ℃, the probio solution of the step of cut-in quality percentage 1% (2) preparation; In 37 ℃ of fermentations 2 days, obtain containing the anticorrisive agent of active probiotic.
(4) detect: cultivate counting with MRS culture medium flat plate anaerobism, wherein the bacterium viable count is no less than 10
8Cfu/ml.The storing of product cold chain.Storage is after 6 months down in 4 ℃, and with the dull and stereotyped anaerobic culture medium cell count of MRS, its bacterium number still reaches 10
8Cfu/ml.
(5) raw fish is dried by the fire to water content 40%～60% with low temperature, then foregoing preservatives is sprayed on the raw fish surface, consumption is 0.2～1ml/50g fillet, the nitrogen-filled packaging sealing.Transported and preserve 6 months under 4 ℃, flesh of fish common pathogen is detected, do not exceed standard.The mouthfeel of the flesh of fish does not normally have stale flavor.And do not use the raw fish pathogenic bacteria of anticorrisive agent to exceed standard in a large number, and has the stale flavor of the flesh of fish.
The anticorrisive agent Nantural non-toxic that contains active probiotic of present embodiment preparation, even can directly eat.Be used for the anticorrosion of raw fish, can make its shelf life reach 6 months.
Embodiment 4
(1) pre-treatment of fish guts: collect the grass carp internal organ, remove the fish courage.Cleaning and removing is removed silt, and draining 1 hour rubbed 1 hour with meat grinder, and the centrifugal 10min of 2000rpm removes the upper strata grease.
(2) preparation of probio solution: cultivate bifidobacterium longum (Bifidobacterium lactis respectively with the cultivation of MRS fluid nutrient medium; Bi07) (available from Danisco A/S BJ Rep Office), lactobacillus fermenti (Lactobacillus fermentum; ATCC 9338) (available from Chinese industrial microorganism fungus kind preservation center) and streptococcus thermophilus (Streptococcus thermophils; CICC 6038) (available from Chinese industrial microorganism fungus kind preservation center) to logarithmic phase; Respectively at the centrifugal 10min of 6000rpm, get deposition, the concentration of using SPSS to adjust bacterium respectively again is 10
8Cfu/ml mixes than 1: 1: 1 according to liquor capacity then, obtains probio solution.
(3) contain the preparation of the raw fish anticorrisive agent of active probiotic: get the grass carp internal organ of step (1) preparation of mass percent 8%, the K of mass percent 0.5%
3PO
43H
2The CH of O, mass percent 0.8%
3The distilled water of the glucose of COONa, mass percent 5% and mass percent 77.7% mixes; 121 ℃ of sterilization 15min are cooled to below 40 ℃, the probio solution of the step of cut-in quality percentage 8% (2) preparation; In 37 ℃ of fermentations 2 days, obtain containing the anticorrisive agent of active probiotic.
(4) detect: cultivate counting with MRS culture medium flat plate anaerobism, wherein the bacterium viable count is no less than 10
8Cfu/ml.The storing of product cold chain.Storage is after 6 months down in 4 ℃, and with the dull and stereotyped anaerobic culture medium cell count of MRS, its bacterium number still reaches 10
8Cfu/ml.
(5) raw fish is dried by the fire to water content 40%～60% with low temperature, then foregoing preservatives is sprayed on the raw fish surface, consumption is 0.2～1ml/50g fillet, the nitrogen-filled packaging sealing.Transported and preserve 6 months under 4 ℃, flesh of fish common pathogen is detected, do not exceed standard.The mouthfeel of the flesh of fish does not normally have stale flavor.And do not use the raw fish pathogenic bacteria of anticorrisive agent to exceed standard in a large number, and has the stale flavor of the flesh of fish.
The anticorrisive agent Nantural non-toxic that contains active probiotic of present embodiment preparation, even can directly eat.Be used for the anticorrosion of raw fish, can make its shelf life reach 6 months.
Embodiment 5
(1) pre-treatment of fish guts: collect the silver carp internal organ, remove the fish courage.Cleaning and removing is removed silt, and draining 0.7 hour rubbed 1 hour with meat grinder, and the centrifugal 10min of 2000rpm removes the upper strata grease.
(2) preparation of probio solution: cultivate bifidobacterium longum (Bifidobacterium lactis respectively with the cultivation of MRS fluid nutrient medium; Bi07) (available from Danisco A/S BJ Rep Office), lactobacillus fermenti (Lactobacillus fermentum; ATCC 9338) (available from Chinese industrial microorganism fungus kind preservation center) and streptococcus thermophilus (Streptococcus thermophils; CICC 6038) (available from Chinese industrial microorganism fungus kind preservation center) to logarithmic phase; Respectively at the centrifugal 8min of 8000rpm, get deposition, the concentration of using SPSS to adjust bacterium respectively again is 10
8Cfu/ml mixes than 1: 1: 1 according to liquor capacity then, obtains probio solution.
(3) contain the preparation of the raw fish anticorrisive agent of active probiotic: get the silver carp internal organ of step (1) preparation of mass percent 5%, the K of mass percent 0.3%
3PO
43H
2The CH of O, mass percent 0.5%
3The distilled water of the glucose of COONa, mass percent 3% and mass percent 86.2% mixes; 121 ℃ of sterilization 15min are cooled to below 40 ℃, the probio solution of the step of cut-in quality percentage 5% (2) preparation; In 37 ℃ of fermentations 2 days, obtain containing the anticorrisive agent of active probiotic.
(4) detect: cultivate counting with MRS culture medium flat plate anaerobism, wherein the bacterium viable count is no less than 10
8Cfu/ml.The storing of product cold chain.Storage is after 6 months down in 4 ℃, and with the dull and stereotyped anaerobic culture medium cell count of MRS, its bacterium number still reaches 10
8Cfu/ml.
(5) raw fish is dried by the fire to water content 40%～60% with low temperature, then foregoing preservatives is sprayed on the raw fish surface, consumption is 0.2～1ml/50g fillet, the nitrogen-filled packaging sealing.Transported and preserve 6 months under 4 ℃, flesh of fish common pathogen is detected, do not exceed standard.The mouthfeel of the flesh of fish does not normally have stale flavor.And do not use the raw fish pathogenic bacteria of anticorrisive agent to exceed standard in a large number, and has the stale flavor of the flesh of fish.
The anticorrisive agent Nantural non-toxic that contains active probiotic of present embodiment preparation, even can directly eat.Be used for the anticorrosion of raw fish, can make its shelf life reach 6 months.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (8)
1. raw fish anticorrisive agent that contains active probiotic is characterized in that containing following composition by mass percentage:
Described fish guts is meant the mixture of the various internal organs except that the fish courage in the fish belly chamber;
Described probio is the mixture that lactobacillus, Bifidobacterium and streptococcus thermophilus form in bacterial population number ratio at 1: 1: 1.
2. according to the said raw fish anticorrisive agent that contains active probiotic of claim 1, it is characterized in that: described fish guts is the internal organ of fresh-water fishes.
3. according to the said raw fish anticorrisive agent that contains active probiotic of claim 2, it is characterized in that: described fresh-water fishes are at least a in Tilapia mossambica, perch, dace, grass carp or the silver carp.
4. each said preparation method who contains the raw fish anticorrisive agent of active probiotic of claim 1～3 is characterized in that comprising following steps:
(1) pre-treatment of fish guts: the fish guts behind the fish courage is removed in collection, cleans up, and draining rubs, and is centrifugal, removes the upper strata grease;
(2) preparation of probio solution: with the probio activation, be cultured to exponential phase, centrifugal, get deposition; Suspend with SPSS then and precipitate, obtain probio solution;
(3) contain the preparation of the raw fish anticorrisive agent of active probiotic: get the fish guts of step (1) preparation of mass percent 2～8%, the K of mass percent 0.3～0.5%
3PO
43H
2The CH of O, mass percent 0.3～0.8%
3The glucose and the water of COONa, mass percent 3～5% evenly mix, sterilization; Then be cooled to below 40 ℃, the probio solution of the step of cut-in quality percentage 1～8% (2) preparation ferments, and obtains containing the raw fish anticorrisive agent of active probiotic; Wherein, fish guts, K
3PO
43H
2O, CH
3The summation of COONa, glucose, probio solution and water is a mass percent 100%.
5. preparation method according to claim 4 is characterized in that:
The time of draining described in the step (1) is 0.5～1 hour;
The condition of rubbing described in the step (1) is for to rub in meat grinder 0.5～1 hour;
Centrifugal condition described in the step (1) is in the centrifugal 5～10min of 2000～5000rpm.
6. preparation method according to claim 4 is characterized in that:
The culture medium of cultivating described in the step (2) is the MRS fluid nutrient medium;
Centrifugal condition described in the step (2) is in the centrifugal 5～10min of 4000～8000rpm;
The concentration of probio solution is 10 described in the step (2)
8CFU/ml.
7. preparation method according to claim 4 is characterized in that:
Water described in the step (3) is deionized water or distilled water;
The condition of sterilization described in the step (3) is in 121 ℃ of 15min at least that sterilize;
The condition of fermentation described in the step (3) is in 30～37 ℃ of fermentations 2 days.
8. each said application that contains the raw fish anticorrisive agent of active probiotic of claim 1～3 is characterized in that: the said raw fish anticorrisive agent of the surface sprinkling 0.2～1ml of every 50g raw fish.
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|---|---|---|---|
| CN2010102493999A CN101919435B (en) | 2010-08-09 | 2010-08-09 | Sashimi preservative containing active probiotics as well as preparation method and application thereof |
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| CN102986827A (en) * | 2012-06-04 | 2013-03-27 | 三伊水产株式会社 | Sashimi cryopreservation method |
| CN114081187A (en) * | 2021-10-21 | 2022-02-25 | 广州泊亚生物科技有限公司 | Preservative-free biological activity application technology |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092942A (en) * | 1993-04-03 | 1994-10-05 | 梁峰 | Compound preservative for fish and meat |
| EP1338205A1 (en) * | 2002-01-04 | 2003-08-27 | Giuliano Zanaglia | Lactobacillus for preservation of food products |
| CN101155910A (en) * | 2004-12-17 | 2008-04-02 | 加拿大生物芯公司 | Lactic acid bacteria for processing food |
| CN101356981A (en) * | 2008-09-09 | 2009-02-04 | 昆山市周庄绿尔康食品有限公司 | Method for pickling fresh water fish |
| CN101433345A (en) * | 2008-12-01 | 2009-05-20 | 江南大学 | Comprehensive utilization method of abodominal organs of grass carp |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101558781B (en) * | 2009-05-14 | 2011-09-14 | 浙江工商大学 | Composite biological antistaling agent for minced fillet product and method for using same |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092942A (en) * | 1993-04-03 | 1994-10-05 | 梁峰 | Compound preservative for fish and meat |
| EP1338205A1 (en) * | 2002-01-04 | 2003-08-27 | Giuliano Zanaglia | Lactobacillus for preservation of food products |
| CN101155910A (en) * | 2004-12-17 | 2008-04-02 | 加拿大生物芯公司 | Lactic acid bacteria for processing food |
| CN101356981A (en) * | 2008-09-09 | 2009-02-04 | 昆山市周庄绿尔康食品有限公司 | Method for pickling fresh water fish |
| CN101433345A (en) * | 2008-12-01 | 2009-05-20 | 江南大学 | Comprehensive utilization method of abodominal organs of grass carp |
Non-Patent Citations (1)
| Title |
|---|
| 段杉等.新型食品防腐剂.《中国食品添加剂》.2002,(第4期),62-65. * |
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