CN101904412A - Method for producing food attractant by using fish soluble extract - Google Patents

Method for producing food attractant by using fish soluble extract Download PDF

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Publication number
CN101904412A
CN101904412A CN2010102237206A CN201010223720A CN101904412A CN 101904412 A CN101904412 A CN 101904412A CN 2010102237206 A CN2010102237206 A CN 2010102237206A CN 201010223720 A CN201010223720 A CN 201010223720A CN 101904412 A CN101904412 A CN 101904412A
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China
Prior art keywords
fish
phagostimulant
soluble extract
molten slurry
fermentation
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CN2010102237206A
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CN101904412B (en
Inventor
陈丹
王建红
范乃盈
蒋洪斌
王华朗
张海涛
刘兴旺
王卓铎
韩垂旺
程成荣
冯耳元
符应琳
王喜波
姜永杰
王蕾蕾
徐志雄
蔡仁贤
王立志
田瑜
郑朝阳
黄智成
张旭娟
赵丽梅
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ZHANJIANG EVERGREEN SPECIAL FEED CO Ltd
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ZHANJIANG EVERGREEN SPECIAL FEED CO Ltd
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Publication of CN101904412A publication Critical patent/CN101904412A/en
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  • Feed For Specific Animals (AREA)

Abstract

The invention relates to the field of feed processing, in particular to a method for producing a food attractant by using a waste fish soluble extract in a processing process of fish meal. The method produces a food attractant capable of improving the feed palatability of aquaculture animals by taking the waste and fish soluble extract from the production of the fish meal as a raw material and by the procedures of steam boiling, mixing, fermentation, drying, and packaging. By the processing and enzymolysis, the polymer proteins in the fish soluble extract are hydrolyzed into small peptides and free amino acids, so the absorption utilization ratio and the food attraction of the fish soluble extract are improved. The method has the advantages of making the waste profitable by using the processing waste from the fish meal as the raw material, reducing the pollution of discharged fish meal to the environment, along with environment friendliness, stable source of the raw material, and simple production process.

Description

A kind of method of utilizing the molten slurry of fish to produce phagostimulant
[technical field]
The present invention relates to field of feed processing, relate in particular to a kind of method of utilizing the molten slurry of waste fish in the fish meal process to produce phagostimulant.
[background technology]
At present, a large amount of palatabilities that cause feed of using of plant protein material descend in the aquatic feeds, therefore the suitable aquatic animal phagostimulant of very necessary exploitation.
The molten slurry of fish is the squeezing waste water that produces in the fish meal production process.In the early stage fish meal production process, the molten slurry of fish directly discharges in environment without reclaiming usually, has caused the serious environmental pollution.In recent years, the existing more research of the method that the molten slurry of relevant fish is recycled, dry (Chinese patent CN200710030735.9) in the fish meal as the molten slurry of fish is added, or be dried to fish soluble slurry protein powder (Chinese patent CN200710120346.5) etc. after handling through de-oiling, unsticking polysaccharide.Because the molten slurry of fish contains soluble protein and free amino acid in the fish soma, and stronger fishy smell flavor is arranged, aquatic livestock is had stronger food calling effect, therefore have potentiality as aquatic attractant.But contain more macro-molecular protein in the molten slurry of fish,, limited its digestive utilization ratio as collagen, peptone etc.By processing and macromolecule protein in the molten slurry of fish is hydrolyzed into little peptide to enzymolysis processing and free amino acid not only can improve its absorption rate, can also improve its food calling effect, therefore its raw materials for production as the aquatic feeds phagostimulant be had stronger feasibility.
[summary of the invention]
The purpose of this invention is to provide a kind of method of utilizing the molten slurry production of fish can improve the phagostimulant of aquatic animal feed palatability.
At above-mentioned purpose, the technical solution used in the present invention is as follows:
A kind of method of utilizing the molten slurry of fish to produce phagostimulant comprises following step:
(1) fish meal is squeezed in the step rustless steel container that injects 500L with the molten slurry of fish after fish oil separates, high temperature hot digestion 30-60 minute;
(2) be cooled to about 37 ℃ through the molten slurry of fish after the boiling, add 1000-2000g trypsase and 500-1000g papain in the 500L container, mix the back 37 ℃ of condition bottom fermentations 3-5 hour;
(3) material after protease fermentation heat once more boil concentrated, stop heating when treating moisture for 50%-60%, add Flavourzyme flavor protease 500-1000g after being cooled to 37 ℃, bacillus 3000-5000g, lactic acid bacteria 3000-5000g, glycine 300-500g, taurine 200-300g mixes the back and continue fermentation 1.5-2 hour under 37 ℃ of condition;
(4) once more the product after the fermentation to be dried to moisture through vacuum drying chamber be 30-35%, obtain phagostimulant of the present invention, inject then to pack in the sealed plastic bucket and get final product.
Final products are grey or light brown slurry, strong flesh of fish fragrance are arranged, free from extraneous odour.
The phagostimulant of the improved aquatic animal feed of gained palatability according to the method described above, the addition in aquatic animal feed is the 3%-6% of aquatic animal feed quality.
The pressed liquor that obtains of squeezing step of preparation fish meal is carried out Separation of Solid and Liquid, and it promptly is fish oil that the liquid part gets upper strata grease through the high speed centrifugation layering, and lower floor promptly is the molten slurry of fish, and the molten slurry of fish with after fish oil separates, is promptly got raw material of the present invention.The present invention has following advantage: (1) phagostimulant after by fermentation is rich in natural ocean amino acids material, can significantly improve the palatability of aquatic livestock to feed; (2) ferment by the secondary fermentation technology, both guaranteed the fully hydrolysis of high molecular weight protein in the molten slurry of fish, guaranteed that again the probio of secondary fermentation can survive in the phagostimulant product; (3) add probiotics preparation, suppressed the breeding of the harmful mushroom in the final products, can improve immunity and the premunition of aquatic livestock simultaneously, certain effect is also arranged improving water quality; (4) nontoxic secondary residual, the no resistance to the action of a drug have reduced the pollution of the molten slurry discharging of fish to environment, environmental protection simultaneously; (5) raw materials enjoy stable sources of this phagostimulant, production technology is simple.
[specific embodiment]
Fish meal is squeezed the molten slurry injection of the fish after Separation of Solid and Liquid 500L rustless steel container in the step, the high temperature heating was cooled to 37 ℃ after 30 minutes, add 1000g trypsase and 1000g papain in the container, mix the back 37 ℃ of condition bottom fermentations 3 hours; Material after protease fermentation heat once more boil concentrated, treat that moisture is to stop heating at 50% o'clock, add Flavourzyme flavor protease 500g, bacillus 5000g, lactic acid bacteria 3000g, glycine 500g, taurine 300g after being cooled to 37 ℃ and mix the back and under 37 ℃ of conditions, continue fermentation 1.5 hours; Product after the fermentation is 30% through vacuum drying to moisture once more, obtains phagostimulant of the present invention, injects then to pack in the sealed plastic bucket to get final product.
This product is used in the Penaeus Vannmei feed according to 5% addition, culture initial body weight and be about the Penaeus Vannmei of 0.28g, through 2 months growth tests, the Penaeus Vannmei that adds phagostimulant of the present invention in the feed improves 21% with the control group that does not add phagostimulant than grazing rate, and specific growth rate improves 15%.
Add phagostimulant 6% of the present invention in cobio prelarva feed, the cobio prelarva about 0.2g cultures that average weight reaches 25g after one month, is significantly higher than and does not add phagostimulant group of the present invention (22g).
Add phagostimulant 3% of the present invention in crab feed, culture through the whole year and use, final crab listing specification accounts for 87% more than 3 liang, is significantly higher than and does not add phagostimulant group of the present invention (68%).

Claims (2)

1. method of utilizing the molten slurry of fish to produce phagostimulant is characterized in that may further comprise the steps:
(1) the molten slurry of fish is injected the rustless steel container of 500L, high temperature hot digestion 30-60 minute;
(2) be cooled to about 37 ℃ through the molten slurry of fish after the boiling, add 1000-2000g trypsase and 500-1000g papain in the 500L container, mix the back 37 ℃ of condition bottom fermentations 3-5 hour;
(3) material after protease fermentation heat once more boil concentrated, stop heating when treating moisture for 50%-60%, add Flavourzyme flavor protease 500-1000g after being cooled to 37 ℃, bacillus 3000-5000g, lactic acid bacteria 3000-5000g, glycine 300-500g, taurine 200-300g mixes the back and continue fermentation 1.5-2 hour under 37 ℃ of condition;
(4) once more the product after the fermentation to be dried to moisture through vacuum drying chamber be 30-35%, promptly get phagostimulant.
2. according to the phagostimulant of the described method gained of claim 1, it is characterized in that the addition of this phagostimulant in aquatic animal feed is the 3%-6% of aquatic animal feed quality.
CN2010102237206A 2010-07-12 2010-07-12 Method for producing food attractant by using fish soluble extract Active CN101904412B (en)

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Application Number Priority Date Filing Date Title
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CN101904412B CN101904412B (en) 2013-01-30

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104872379A (en) * 2015-05-18 2015-09-02 青岛尚德生物技术有限公司 Fermentation substance prepared by adding fish soluble in corn-soybean meal diet and fermentation method of fermentation substance
CN106804879A (en) * 2016-12-26 2017-06-09 广东希普生物科技股份有限公司 A kind of hydrolysis, the preparation method and applications of enzymolysis animal protein feed
CN107242400A (en) * 2017-07-11 2017-10-13 浙江丰宇海洋生物制品有限公司 A kind of method of the molten slurry production phagostimulant of utilization fish
CN107333969A (en) * 2017-07-11 2017-11-10 浙江丰宇海洋生物制品有限公司 A kind of phagostimulant using the molten slurry production of fish
CN107494886A (en) * 2017-07-11 2017-12-22 浙江丰宇海洋生物制品有限公司 A kind of albumen powder product using the molten slurry production of fish
CN108902466A (en) * 2018-07-18 2018-11-30 岭商(浙江)生物科技有限公司 A kind of molten slurry of hydrolysis fish and its production method and application
CN109221808A (en) * 2018-09-26 2019-01-18 何秋宏 A kind of preparation method of fish feed additive
CN112155121A (en) * 2020-11-11 2021-01-01 广东海洋大学 Feed for enhancing immunity of tilapia and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1175362A (en) * 1996-09-03 1998-03-11 张玉和 Active fish meal
CN1488277A (en) * 2003-07-02 2004-04-14 江南大学 Fodder phagostimulant compounded with fish extract and preparing method use thereof
CN1726802A (en) * 2005-07-29 2006-02-01 中国水产科学研究院黄海水产研究所 The prescription of left-eyed flounder micro-granulated feed and compound method
CN101120722A (en) * 2007-08-16 2008-02-13 北京英惠尔生物技术有限公司 Fish soluble slurry protein powder and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1175362A (en) * 1996-09-03 1998-03-11 张玉和 Active fish meal
CN1488277A (en) * 2003-07-02 2004-04-14 江南大学 Fodder phagostimulant compounded with fish extract and preparing method use thereof
CN1726802A (en) * 2005-07-29 2006-02-01 中国水产科学研究院黄海水产研究所 The prescription of left-eyed flounder micro-granulated feed and compound method
CN101120722A (en) * 2007-08-16 2008-02-13 北京英惠尔生物技术有限公司 Fish soluble slurry protein powder and preparation method thereof

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104872379A (en) * 2015-05-18 2015-09-02 青岛尚德生物技术有限公司 Fermentation substance prepared by adding fish soluble in corn-soybean meal diet and fermentation method of fermentation substance
CN104872379B (en) * 2015-05-18 2018-07-24 青岛尚德生物技术有限公司 A kind of fermentate and its fermentation process adding the molten slurry of fish in corn-soybean meal diet
CN106804879A (en) * 2016-12-26 2017-06-09 广东希普生物科技股份有限公司 A kind of hydrolysis, the preparation method and applications of enzymolysis animal protein feed
CN107242400A (en) * 2017-07-11 2017-10-13 浙江丰宇海洋生物制品有限公司 A kind of method of the molten slurry production phagostimulant of utilization fish
CN107333969A (en) * 2017-07-11 2017-11-10 浙江丰宇海洋生物制品有限公司 A kind of phagostimulant using the molten slurry production of fish
CN107494886A (en) * 2017-07-11 2017-12-22 浙江丰宇海洋生物制品有限公司 A kind of albumen powder product using the molten slurry production of fish
CN108902466A (en) * 2018-07-18 2018-11-30 岭商(浙江)生物科技有限公司 A kind of molten slurry of hydrolysis fish and its production method and application
CN109221808A (en) * 2018-09-26 2019-01-18 何秋宏 A kind of preparation method of fish feed additive
CN112155121A (en) * 2020-11-11 2021-01-01 广东海洋大学 Feed for enhancing immunity of tilapia and preparation method thereof

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