CN101889202A - 用于自动预处理糖链的仪器 - Google Patents
用于自动预处理糖链的仪器 Download PDFInfo
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- CN101889202A CN101889202A CN2008801193593A CN200880119359A CN101889202A CN 101889202 A CN101889202 A CN 101889202A CN 2008801193593 A CN2008801193593 A CN 2008801193593A CN 200880119359 A CN200880119359 A CN 200880119359A CN 101889202 A CN101889202 A CN 101889202A
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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Abstract
Description
4℃的试剂架 | mL | 洗涤阀 | mL |
胰蛋白酶 | 0.48 | 2%AcO H的ACN溶液 | 34.56 |
PNGaseF | 0.48 | 2M盐酸胍 | 38.4 |
水 | 86.4 | ||
试剂架 | mL | 1%TEA | 38.4 |
PHL | 1.92 | 10mM HCl | 38.4 |
4℃的试剂架 | mL | 洗涤阀 | mL |
DTT | 0.48 | MeOH | 38.4 |
IAA | 0.96 | 二氧六环 | 57.6 |
10%Ac2O | 9.6 | 95%ACN | 72.96 |
MTT | 9.6 | ||
20mM试剂 | 1.92 | ||
5%ACN | 9.6 |
Claims (20)
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JP2007262680 | 2007-10-05 | ||
JP2007262771 | 2007-10-05 | ||
JP262680/2007 | 2007-10-05 | ||
JP262771/2007 | 2007-10-05 | ||
PCT/JP2008/068111 WO2009044900A1 (ja) | 2007-10-05 | 2008-10-03 | 糖鎖自動前処理装置 |
Publications (2)
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CN101889202A true CN101889202A (zh) | 2010-11-17 |
CN101889202B CN101889202B (zh) | 2013-02-06 |
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US (1) | US8877454B2 (zh) |
EP (1) | EP2207030A4 (zh) |
JP (1) | JP5396277B2 (zh) |
KR (1) | KR20100098500A (zh) |
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Cited By (5)
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CN106662555A (zh) * | 2014-08-12 | 2017-05-10 | 株式会社爱思迪科学 | 氨基酸、有机酸和糖质的分析预处理装置及分析预处理方法 |
CN107271593A (zh) * | 2017-06-19 | 2017-10-20 | 西北大学 | 还原性糖链的靶板衍生化和maldi‑tof‑ms分析方法 |
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CN110038652A (zh) * | 2014-09-11 | 2019-07-23 | 克忧健康公司 | 用于检测和量化分析物的***和方法 |
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CN102144164A (zh) * | 2008-04-30 | 2011-08-03 | 住友电木株式会社 | 标记糖链的方法 |
WO2010018834A1 (ja) | 2008-08-12 | 2010-02-18 | 国立大学法人 北海道大学 | アンモニウム塩による還元性糖鎖遊離方法 |
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JP5392500B2 (ja) * | 2010-03-18 | 2014-01-22 | 住友ベークライト株式会社 | 質量分析による糖鎖の分析方法 |
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JP2013081456A (ja) * | 2011-09-29 | 2013-05-09 | Sumitomo Bakelite Co Ltd | 糖鎖精製装置および糖鎖精製方法 |
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JP5396277B2 (ja) | 2014-01-22 |
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