CN101880649B - 一种干细胞培养方法 - Google Patents
一种干细胞培养方法 Download PDFInfo
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- CN101880649B CN101880649B CN200910050582A CN200910050582A CN101880649B CN 101880649 B CN101880649 B CN 101880649B CN 200910050582 A CN200910050582 A CN 200910050582A CN 200910050582 A CN200910050582 A CN 200910050582A CN 101880649 B CN101880649 B CN 101880649B
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- stem cell
- cell culture
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- epithelial cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells
- C12N2502/025—Coculture with; Conditioned medium produced by embryonic cells extra-embryonic cells, e.g. amniotic epithelium, placental cells, Wharton's jelly
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Gynecology & Obstetrics (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (10)
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200910050582A CN101880649B (zh) | 2009-05-05 | 2009-05-05 | 一种干细胞培养方法 |
EP10771959A EP2428562A4 (en) | 2009-05-05 | 2010-05-04 | METHOD OF STEM CELL CULTIVATION |
PCT/CN2010/000616 WO2010127555A1 (zh) | 2009-05-05 | 2010-05-04 | 一种干细胞培养方法 |
US13/379,651 US9163214B2 (en) | 2009-05-05 | 2010-05-04 | Method for culturing stem cells |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200910050582A CN101880649B (zh) | 2009-05-05 | 2009-05-05 | 一种干细胞培养方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101880649A CN101880649A (zh) | 2010-11-10 |
CN101880649B true CN101880649B (zh) | 2012-09-05 |
Family
ID=43049950
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200910050582A Active CN101880649B (zh) | 2009-05-05 | 2009-05-05 | 一种干细胞培养方法 |
Country Status (4)
Country | Link |
---|---|
US (1) | US9163214B2 (zh) |
EP (1) | EP2428562A4 (zh) |
CN (1) | CN101880649B (zh) |
WO (1) | WO2010127555A1 (zh) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2014207618A1 (en) | 2013-01-16 | 2015-08-06 | Emory University | Cas9-nucleic acid complexes and uses related thereto |
CN103114075B (zh) * | 2013-01-18 | 2014-11-12 | 内蒙古大学 | 一种牛滋养层干细胞建系方法 |
US20160060655A1 (en) | 2014-05-30 | 2016-03-03 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods to treat latent viral infections |
US10117911B2 (en) | 2015-05-29 | 2018-11-06 | Agenovir Corporation | Compositions and methods to treat herpes simplex virus infections |
CN110079497B (zh) * | 2019-03-20 | 2022-05-13 | 济南赛尔生物科技股份有限公司 | 一种脂肪干细胞的分离与培养方法 |
CN111172105A (zh) * | 2020-01-17 | 2020-05-19 | 中国人民解放军联勤保障部队第九二〇医院 | 一种间充质干细胞分泌素的制备方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1590541A (zh) * | 2004-05-27 | 2005-03-09 | 天津医科大学眼科中心 | 角膜缘干细胞组织工程复合体及其制备方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1445363A (zh) * | 2003-01-17 | 2003-10-01 | 中山大学 | 体外诱导人胚胎干细胞定向分化为表皮干细胞的方法 |
US20060275899A1 (en) * | 2004-12-30 | 2006-12-07 | Stemlifeline, Inc. | Methods and compositions relating to embryonic stem cell lines |
CN102725399B (zh) * | 2009-12-18 | 2015-03-11 | 上海赛傲生物技术有限公司 | 制备多能干细胞的材料和方法 |
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2009
- 2009-05-05 CN CN200910050582A patent/CN101880649B/zh active Active
-
2010
- 2010-05-04 US US13/379,651 patent/US9163214B2/en not_active Expired - Fee Related
- 2010-05-04 WO PCT/CN2010/000616 patent/WO2010127555A1/zh active Application Filing
- 2010-05-04 EP EP10771959A patent/EP2428562A4/en not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1590541A (zh) * | 2004-05-27 | 2005-03-09 | 天津医科大学眼科中心 | 角膜缘干细胞组织工程复合体及其制备方法 |
Non-Patent Citations (5)
Title |
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Martin Grueterich et al..Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane.《Investigative ophthalmology & visual science》.2002,第43卷(第1期),63-71. |
Martin Grueterich et al..Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane.《Investigative ophthalmology & * |
visual science》.2002,第43卷(第1期),63-71. * |
去上皮羊膜上培养兔角膜缘干细胞的实验研究;宋曲园等;《宁夏医学院学报》;20080630;第30卷(第3期);摘要、第293页左栏第1.2部分、右栏第1.3.2-1.3.3部分、第294页左栏第2.3部分、右栏倒数第二段 * |
宋曲园等.去上皮羊膜上培养兔角膜缘干细胞的实验研究.《宁夏医学院学报》.2008,第30卷(第3期),摘要、第293页左栏第1.2部分、右栏第1.3.2-1.3.3部分、第294页左栏第2.3部分、右栏倒数第二段. |
Also Published As
Publication number | Publication date |
---|---|
US20120122213A1 (en) | 2012-05-17 |
US9163214B2 (en) | 2015-10-20 |
EP2428562A1 (en) | 2012-03-14 |
CN101880649A (zh) | 2010-11-10 |
WO2010127555A1 (zh) | 2010-11-11 |
EP2428562A4 (en) | 2013-03-27 |
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Legal Events
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
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ASS | Succession or assignment of patent right |
Owner name: ZHONGMEI SAIAO (SHANGHAI) BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: HEHONG BIO-TECH (SHANGHAI) CO., LTD. Effective date: 20120306 |
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Effective date of registration: 20120306 Address after: 200030 Xuhui District, Hengshan Road, No. 910, Applicant after: The International Peace Maternity & Child Health Hospital of China Welfare Institute Co-applicant after: Cell-star Biological Technology (Shanghai) Co., Ltd. Co-applicant after: Guolian (Shanghai) Biotechnology Research Co., Ltd. Address before: 200030 Xuhui District, Hengshan Road, No. 910, Applicant before: The International Peace Maternity & Child Health Hospital of China Welfare Institute Co-applicant before: Hehong Biotechnology (Shanghai) Co., Ltd. |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee | ||
CP01 | Change in the name or title of a patent holder |
Address after: 200030 Xuhui District, Hengshan Road, No. 910, Patentee after: The International Peace Maternity & Child Health Hospital of China Welfare Institute Patentee after: SINO-AMERICAN ICELL (SHANGHAI) BIOTECHNOLOGY CO., LTD. Patentee after: Shanghai United Stem Cell Technology Co., Ltd. Address before: 200030 Xuhui District, Hengshan Road, No. 910, Patentee before: The International Peace Maternity & Child Health Hospital of China Welfare Institute Patentee before: Cell-star Biological Technology (Shanghai) Co., Ltd. Patentee before: Guolian (Shanghai) Biotechnology Research Co., Ltd. |