CN101865849B - weak light detection device - Google Patents
weak light detection device Download PDFInfo
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- CN101865849B CN101865849B CN2009100823173A CN200910082317A CN101865849B CN 101865849 B CN101865849 B CN 101865849B CN 2009100823173 A CN2009100823173 A CN 2009100823173A CN 200910082317 A CN200910082317 A CN 200910082317A CN 101865849 B CN101865849 B CN 101865849B
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- reaction cup
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- weak light
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- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 claims description 99
- 238000005259 measurement Methods 0.000 claims description 47
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- 238000003556 assay Methods 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 5
- 239000002245 particle Substances 0.000 abstract description 3
- 238000003018 immunoassay Methods 0.000 abstract description 2
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- 238000012360 testing method Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
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- 238000005516 engineering process Methods 0.000 description 2
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- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
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- 238000012372 quality testing Methods 0.000 description 1
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Abstract
The invention relates to a weak light detection device, belonging to the field of chemiluminescent immunoassay analyzers. The device comprises a measuring hood and a photoelectric sensor. An assay cup inlet and an assay cup outlet are arranged on the wall of the measuring hood. The device is characterized in that a measuring rotating disc closely attached onto the inner wall of the measuring hood is arranged in the measuring hood, an assay cup holding hole which can be communicated with the assay cup inlet and the assay cup outlet through rotation is arranged on the measuring rotating disc, a measuring port is arranged on the wall of the measuring hood, one end of the measuring port right faces to the rotating trajectory of the mouth of an assay cup in the holding hole, the photoelectric sensor is installed at the other end of the measuring port, the measuring port and the holding hole on the measuring rotating disc are not communicated with the assay cup inlet or the assay cup outlet. Thereby natural light is almost disappeared when the natural light reach the photoelectric sensor at the other end of a measuring chamber, the interference of the natural light is eliminated, the noise of the background for detecting luminescent particles is low and the accuracy of the detected data is greatly improved. The entire detection process is conducted in a circulating device which has the advantages of simple structure and easy automation realization.
Description
Technical field
The present invention relates to medicine equipment detection technique field, particularly a kind of Weak light detection device that is used for chemical illumination immunity analysis instrument.
Background technology
In recent years, progress along with social development and biomedical technology, the chemiluminescence immune assay amount detection that carries out every year is all increasing considerably, and is not only urgent day by day to the requirement of personalized fast detecting, and also very high to the accuracy and the accuracy requirement of quality testing data.It is to utilize the idiosyncrasy of antigen and antibody that chemical illumination immunity analysis instrument detects principle, the free energy that this biochemical reaction discharges excites intermediate, get back to excited state from ground state, energy discharges with the form of photon, photoelectric commutator converts faint optical signal to discernible electric signal, carries out the signal scaling processing by corresponding device thereof again and finally converts the photogenic granules subnumber to.Obviously, carrying out the lucifuge measure is the key link that guarantees to detect quality.In the prior art, the pick-up unit that is applied to the chemiluminescence immunoassay checkout equipment adopts the measure of totally enclosed type lucifuge more, be difficult for realizing robotization, can not satisfy the needs of in batches extensive test item, if the lucifuge method is improper, then detect the accuracy and the precision of data in the natural light meeting disturbed specimen testing process.Therefore, market in urgent need is the Weak light detection device that antijamming capability is strong, highly sensitive, degree of accuracy is high, treatment effeciency is high.
Summary of the invention
At above-mentioned field, the invention provides a kind of Weak light detection device that adopts labyrinth zig zag plane lucifuge principle design, it is flexible not only to operate, and this pick-up unit possess simple in structure, lucifuge is reliable, treatment effeciency is high, almost do not have natural light to disturb, can reach very high degree of accuracy.
Weak light detection device, comprise and measure cover and photoelectric sensor, measure and have reaction cup import and reaction cup outlet on the wall that covers, it is characterized in that: have one to be close to the measurement rotating disk of measuring the cover inwall in the described measurement cover, there is one can hold the hole with the reaction cup that reaction cup import and outlet connect on the described measurement rotating disk by its rotation, there is one to measure mouth on the wall of described measurement cover, described measurement mouth one is rectified holding hole internal reaction cup rim of a cup rotary motion trace, the other end of measuring mouth is equipped with photoelectric sensor, measures mouth, holding the outlet of hole and reaction cup import or reaction cup does not connect.
Described measurement cover inwall and the gap of measuring between the rotating disk are 0.3-1mm.
Described appearance hole, reaction cup import and reaction cup outlet connect mutually.
The described internal reaction cup rim of a cup position of measuring mouthful when measuring rotating disk and change 180 °, appearance hole.
Described reaction cup import and reaction cup outlet are upper and lower perforation, or front and back connect.
The rotating shaft of described measurement rotating disk is a vertical direction, and described measurement mouth is positioned at the upper wall of measuring cover.
Described measurement cover is square, and described measurement rotating disk is square, and described reaction cup is held the side that the hole is positioned at rotating shaft.
Described reaction cup import and reaction cup outlet are upper and lower perforation, and the position over against the reaction cup import under the described measurement cover is provided with push rod, and described appearance has the shell fragment that can block reaction cup in the hole.
This device also is provided with abandons a glass conduit, and the described glass conduit of abandoning is communicated with the reaction cup outlet.
There is the measurement sleeve pipe outside of described photoelectric sensor.
Thinking of the present invention is reaction cup can be entered in the measurement cover with automatic ordering form, detect, but because the effect of light of importing and exporting can exert an influence to measurement, therefore the present invention has designed a measurement rotating disk, when reaction cup enters when measuring cover from import, it is actually and has entered the appearance hole site of measuring on the rotating disk, leave import by rotation and arrive the position of measuring mouth, after finishing, measurement arrives the exit position by rotation, owing to measure rotating disk near measuring the cover inwall, the anglec of rotation can arbitraryly be set, or/and just not connecting, entrance location can not realize lucifuge with outlet as long as measure mouth, yet natural light can inevitably the exposed section---the reaction cup entrance and exit be injected up and down from measuring cover in the measuring process, (measurement environment light source background requires less than 100) that the requirement of faint light measuring condition is carried out under the lucifuge condition, corpuscular property principle based on the wave-particle duality of light, we have adopted the lucifuge structural design of above-mentioned labyrinth zig zag plane formula to see shown in Figure 2, injecting the natural light measured in the cover mainly is under the reflection case by reflection repeatedly with refraction in the zig zag plane of labyrinth, graded progressively weakens successively, and the photoelectric sensor place that arrives the measuring chamber other end almost completely disappears.
Measure gap less (between the 0.3-1mm) between cover and the measurement rotating disk in it, this lucifuge structure of design is contactless, and close property face does not have the dead contact matching of subsides.
From measuring rotating disk, import, outlet and measurement mouth can be the different angles that are positioned at rotating disk, for making things convenient for operation of equipment, the passage that import and export and appearance hole can be designed to connect, reaction cup is from here advancing, go out over there, more preferably measure mouthful the internal reaction cup rim of a cup position, appearance hole when measuring rotating disk and change 180 °.When entering from import, reaction cup holds the hole, measure rotating disk and drive 180 ° of appearance hole rotations, the reaction cup rim of a cup is over against surveying the inspection mouth, the faint light that luminous substrate is sent in the reagent cup is through being caught by high sensitivity photoelectric sensor in the measuring chamber, carry out a series of opto-electronic conversion, the A/D conversion, each decides to measure its data interval time, and data are sent to host computer.When measuring task is finished, measure rotating disk along the axle center reverse rotation, get back to initial original position, reaction cup withdraws from from outlet.
Reaction cup can be from the turnover of the front and back of horizontal direction, or from the upper and lower discrepancy of vertical direction.
The rotating shaft of measuring rotating disk is then measured mouth and can be positioned at the upper wall of measuring cover as being designed to vertical direction.
Do not have special requirement for the shape of measuring cover and measuring rotating disk, only require that two main bodys are close mutually, guarantee to rotate freely, the slit is as far as possible little simultaneously, makes lacking that light penetrates.The preferred cover of measuring is for square, and it is circular measuring rotating disk, holds the hole and is positioned at the eccentric side of rotating shaft.
Be the convenience of detection and the feasibility of operation, import and export is designed to upper and lower perforation, reaction cup is headed into from lower inlet by push rod holds the hole, push rod is return, shell fragment blocks reaction cup and makes its not rollback, measures dial rotation and makes reaction cup be the detection mouth, and detection finishes, measure rotating disk and be inverted to original position, push rod makes reaction cup eject from outlet.
Can design one and abandon a glass conduit, make from the reaction cup of coming out to advance to abandon glass conduit, it is sent in the discarded bucket.
For guaranteeing the photophobism of photoelectric sensor, can be equipped with the measurement sleeve pipe of lucifuge effect in the outside of photoelectric sensor.
Whole testing process is carried out in a loop structure device, and its behaviour is simple in structure, easily is automated.Because the lucifuge structural design of above-mentioned labyrinth zig zag plane formula has been got rid of the interference of natural light, the background noise that detects incandescnet particle is very little, has improved the detection data accuracy greatly.The present invention reaches best lucifuge effect with the control mode of the most simple and mechanical structure most convenient.
Description of drawings
Fig. 1 Weak light detection device structural representation,
The corpuscular property synoptic diagram of Fig. 2 light,
When detecting, measures Fig. 3 the disk position synoptic diagram,
Fig. 4 measures rotating disk and holds the hole synoptic diagram,
Each label lists as follows among the figure:
The 1-push rod, 2-measures cover, the 3-reaction cup, 4-abandons a glass conduit, the 5-photoelectric sensor, 6-measures sleeve pipe, and 7-measures rotating disk, the import of 8-reaction cup, the outlet of 9-reaction cup, 10-measures mouth, and 11-holds hole, 12-shell fragment.
Embodiment
As shown in Figure 1, Weak light detection device, comprise and measure cover 2 and photoelectric sensor 5, have reaction cup import 8 and reaction cup outlet 9 on the wall of measurement cover 1, it is characterized in that: have one in the described measurement cover 2 near the measurement rotating disk 7 of measuring cover 2 inwalls, have on the described measurement rotating disk 7 one when it rotates and the reaction cup that connect of reaction cup import 8 or reaction cup outlet 9 hold holes 11, there is one to measure mouth 10 on the wall of described measurement cover 2, described measurement mouth 10 1 is rectified holding hole 11 internal reaction cup rim of a cup rotary motion trace, the other end of measuring mouth 10 is equipped with photoelectric sensor 5, the outside of photoelectric sensor 5 is useful on the measurement sleeve pipe 6 of lucifuge, measures mouth 10, holding hole 11 does not connect with reaction cup import 8 or reaction cup outlet 9.When reaction cup 3 enters when measuring cover 2 from reaction cup import 8, it is actually and has entered 11 positions of measuring on the rotating disk 7, appearance hole, leave reaction cup import 8 by the revolving reaction cup and arrive the position of measuring mouth 10, arrive reaction cup by rotation after measurement is finished and export 9 positions, make like this when measuring, can not shine directly into reaction cup influence detection from reaction cup import 8 or reaction cup outlet 9 light that enter.
The gap that described measurement is covered between 2 inwalls and the measurement rotating disk 7 is 0.3-1mm.So little gap, the light complications seldom can enter into the detection position.(as shown in Figure 2)
The present invention will hold hole 11, reaction cup import 8 and reaction cup outlet 9 and be designed to mutual perforation.Promptly measure 11 original positions, appearance hole in the rotating disk 7 and face reaction cup import 8 and reaction cup outlet 9, still get back to the original position after finishing detection.
Described mouthfuls 10 11 internal reaction cups, the 3 rim of a cup positions, appearance hole when measuring rotating disk 7 and change 180 ° of measuring.It is identical and farthest, the light amount of injecting is minimum from the position of reaction cup import 8 and reaction cup outlet 9 promptly to measure reaction cup 3 in the appearance hole of rotating disk 7.(as shown in Figure 3)
Described reaction cup import 8 and reaction cup outlet 9 can be upper and lower perforation, or front and back connect, and the rotating shaft of described measurement rotating disk 7 is a vertical direction, measure mouth 10 and are positioned at the top of measuring cover 2.Reaction cup 3 enters from reaction cup import 8 holds hole 11, measure rotating disk 7 and drive 180 ° of appearance hole 11 rotations, reaction cup 3 rims of a cup are over against measuring mouth 10, the faint light that luminous substrate is sent in the reagent cup is through being caught by high sensitivity photoelectric sensor in the measuring chamber, carry out a series of opto-electronic conversion, the A/D conversion, each decides to measure its data interval time, and data are sent to host computer.When measuring task is finished, measure rotating disk 7 along the axle center reverse rotation, get back to initial original position, reaction cup 3 withdraws from from reaction cup outlet 9.
Described measurement cover 2 can be different shape, is preferably squarely, and described measurement rotating disk 7 is square, and described reaction cup 3 is held holes 11 and is positioned at rotating shaft one side of measuring rotating disk 7.
Described reaction cup import 8 and reaction cup outlet 9 are upper and lower perforation, and 2 times positions over against reaction cup import 8 of described measurement cover are provided with push rod 1, and shell fragment 12 (see figure 4)s that can block reaction cup 3 are arranged in the described appearance hole 11.Reaction cup 3 is pushed up the people by push rod 1 and is held hole 11 from the bottom, push rod 1 is return, and shell fragment 12 blocks reaction cup 3 and do not fall down, and measures rotating disk 7 rotations and makes reaction cup 3 arrive measurement mouth 10, and detection finishes, and measures rotating disk 7 and is inverted to original position, and push rod 1 ejects reaction cup 3.
This device also is provided with abandons glass conduit 4, and the described glass conduit 4 of abandoning is communicated with reaction cup outlet 9, and the reaction cup 3 that ejects is advanced to abandon in glass conduit 4, and it is sent in the discarded bucket.
The present invention has passed through through its lucifuge effect multianalysis of prototyping testing, practice result proves, simple in structure being suitable for, lucifuge is respond well, satisfy the lucifuge requirement of chemical illumination immunity analysis instrument pick-up unit fully, solved the lucifuge complex structure that exists in the prior art, if the lucifuge improper measures, easily cause the technical matters of natural light interference experiment data accuracy, be suitable for promoting.
Claims (10)
1. Weak light detection device, comprise and measure cover and photoelectric sensor, measure and have reaction cup import and reaction cup outlet on the wall that covers, it is characterized in that: have one to be close to the measurement rotating disk of measuring the cover inwall in the described measurement cover, there is one can hold the hole with the reaction cup that reaction cup import and outlet connect on the described measurement rotating disk by its rotation, there is one to measure mouth on the wall of described measurement cover, described measurement mouth one is rectified holding hole internal reaction cup rim of a cup rotary motion trace, the other end of measuring mouth is equipped with photoelectric sensor, measures mouth, holding the outlet of hole and reaction cup import or reaction cup does not connect.
2. Weak light detection device according to claim 1 is characterized in that: described measurement cover inwall and the gap of measuring between the rotating disk are 0.3-1mm.
3. Weak light detection device according to claim 1 is characterized in that: described appearance hole, reaction cup import and reaction cup outlet connect mutually.
4. Weak light detection device according to claim 3 is characterized in that: the described internal reaction cup rim of a cup position of measuring mouthful when measuring rotating disk and change 180 °, appearance hole.
5. Weak light detection device according to claim 4 is characterized in that: described reaction cup import and reaction cup outlet are upper and lower perforation, or front and back connect.
6. Weak light detection device according to claim 5 is characterized in that: the rotating shaft of described measurement rotating disk is a vertical direction, and described measurement mouth is positioned at the upper wall of measuring cover.
7. Weak light detection device according to claim 6 is characterized in that: described measurement cover is square, and described measurement rotating disk is circular, and described reaction cup is held the hole and is positioned at the eccentric side of rotating shaft.
8. Weak light detection device according to claim 7, it is characterized in that: described reaction cup import and reaction cup outlet are upper and lower perforation, position over against the reaction cup import under the described measurement cover is provided with push rod, and described appearance has the shell fragment that can block reaction cup in the hole.
9. Weak light detection device according to claim 1 is characterized in that: also be provided with and abandon a glass conduit, the described glass conduit of abandoning is communicated with the reaction cup outlet.
10. Weak light detection device according to claim 1 is characterized in that: there is the measurement sleeve pipe outside of described photoelectric sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100823173A CN101865849B (en) | 2009-04-14 | 2009-04-14 | weak light detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100823173A CN101865849B (en) | 2009-04-14 | 2009-04-14 | weak light detection device |
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| CN101865849A CN101865849A (en) | 2010-10-20 |
| CN101865849B true CN101865849B (en) | 2011-12-28 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102928344B (en) * | 2012-10-26 | 2014-12-31 | 湖北三江航天红峰控制有限公司 | Locking mechanism for photoelectric sensor |
| CN105961049B (en) * | 2016-05-25 | 2019-01-01 | 河北大学 | A kind of optomagnetic processing unit of Ultra-weak Bioluminescence research |
| CN106198505A (en) * | 2016-08-31 | 2016-12-07 | 四川迈克生物医疗电子有限公司 | Sample testing apparatus and in-vitro diagnosis equipment |
| CN107703108B (en) * | 2017-06-29 | 2024-05-31 | 迈克医疗电子有限公司 | Sample detection device and in-vitro diagnosis equipment |
| WO2019060373A1 (en) * | 2017-09-19 | 2019-03-28 | Beckman Coulter, Inc. | Analog light measuring and photon counting in chemiluminescence measurements |
| CN109580591B (en) * | 2017-09-28 | 2022-03-04 | 深圳市新产业生物医学工程股份有限公司 | Measuring chamber and chemiluminescence detector |
| CN111729908B (en) * | 2020-07-22 | 2024-06-14 | 基蛋生物科技股份有限公司 | Reaction cup cleaning and measuring integrated device |
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