CN101861878A - Insecticidal and acaricidal biological pesticide - Google Patents
Insecticidal and acaricidal biological pesticide Download PDFInfo
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- CN101861878A CN101861878A CN 201010203025 CN201010203025A CN101861878A CN 101861878 A CN101861878 A CN 101861878A CN 201010203025 CN201010203025 CN 201010203025 CN 201010203025 A CN201010203025 A CN 201010203025A CN 101861878 A CN101861878 A CN 101861878A
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Abstract
The invention discloses insecticidal and acaricidal biological pesticide, which belongs to the technical field of pesticide. Active ingredients of the pesticide comprise Abamectin and Beauveria bassiana, particularly the Beauveria bassiana content is 5 to 50 billion spores per gram preparation, and the Abamectin content is 0.5 to 20 weight percent. The formulations such as wettable powder, oil suspension and the like can be prepared by adding proper auxiliary materials into the biological pesticide. A series of indoor screening experiments and field experiments show that the compound of the Abamectin and the Beauveria bassiana has remarkable synergy, can be acted on two different targets at the same time to poison and attack pests, is particularly suitable for multiple agricultural pests such as lepidoptera, coleoptera, homoptera and acarina pests, root-knot nematode and the like against organic chemical pesticide, is safe for living beings such as people, animal, natural enemy and the like, has good compatibility with the environment, and belongs to broad-spectrum efficient biological pesticide.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of biopesticide of Insecticiding-miticiding.
Background technology
The forties in 20th century, the appearance of synthetic organic pesticide has greatly strengthened the ability of the sick worm harm of human control, has made great contribution for we retrieve the crop yield loss.But long-term the dependence and a large amount of synthetic organic pesticides that use brought a series of problem demanding prompt solutions, as unbalance, the food security of environmental pollution, ecotope etc., brings many adverse influences for the sustainable development of agricultural economy.
Along with continuous progress in science and technology; reduce and use the cry of synthetic organic pesticide, protection environment for human survival to grow to even greater heights; research and development utilize biopesticide control diseases and pests of agronomic crop, develop into one of important subject of domestic and international plant protection scientific worker.Biopesticide have selectivity strong, to the person poultry safety; easily degraded, little to eco-environmental impact; can bring out the insect epidemic disease; can utilize agricultural byproducts production and processing, wide material sources, advantage such as with low cost; with preserve the ecological environment and the requirement of society coordinated development matches, will ensure grain security, improve the people ' s health level, alleviate energy pressure, many aspects such as improve the ecological environment are bringing into play important, irreplaceable effect.
Summary of the invention
The objective of the invention is for a kind of biopesticide of Insecticiding-miticiding is provided.
Based on this purpose, the technical solution used in the present invention is: a kind of biopesticide of Insecticiding-miticiding, its effective ingredient are Avermectin (Abamectin) and beauveria bassiana (Beauveria bassiana).
The content of beauveria bassiana is 50～50,000,000,000 spores/restraint agent in the described biopesticide, and the content of Avermectin is 0.5～20wt%.
Described biopesticide is a wetting powder, contains beauveria bassiana 50～50,000,000,000 spores/restraint agent, Avermectin 0.5～20wt%, wetting agent 0.5～5wt%, dispersant 1～10wt%, and all the other supply 100% by filler.
In the described wetting powder, wetting agent is for drawing back powder, wetting and penetrating agent T or neopelex.
In the described wetting powder, dispersant is NNO, MF or sodium lignin sulfonate.
In the described wetting powder, filler is kaolin, bentonite, white carbon or atlapulgite.
Described biopesticide is an oil-suspending agent, contains beauveria bassiana 50～40,000,000,000 spores/restraint agent, Avermectin 0.5～10wt%, thickener 0.1～0.5wt%, dispersant 0.5～3wt%, surfactant 5～15wt%, and all the other supply 100% by solvent.
In the described oil-suspending agent, thickener is carboxymethyl cellulose, sodium carboxymethylcellulose or xanthans.
In the described oil-suspending agent, dispersant is NNO, MF or sodium lignin sulfonate.
In the described oil-suspending agent, surfactant fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene or tween;
Described solvent is vegetable oil, epoxidized soybean oil or methyl oleate.
Biopesticide of the present invention is by Avermectin and two kinds of composite forming of active ingredient of beauveria bassiana, can be made into formulations such as wetting powder, oil-suspending agent after adding suitable auxiliary material.Wherein, Avermectin is the natural products that separates from edaphon, belongs to macrolide disaccharide compounds; Insect and mite class had tag and stomach poison function, and faint fumigation action is arranged; No systemic action, but blade is had very strong osmosis, can kill subepidermal insect, the longevity of residure is long; Do not kill ovum.Different with the mechanism of action of general insecticide is, Avermectin is disturbed the neurophysiology activity, stimulate and discharge the r-aminobutyric acid, and the r-aminobutyric acid has inhibitory action to arthropodan nerve conduction, become mite, if mite, insect and larva thereof with paralysis symptom promptly appears after medicament contacts, inertia is not got food, and is dead after 2～4 days.Because of not causing that insect dewaters rapidly, so its lethal effect is slower.Though Avermectin has certain lethal effect to predatism and parasite, the plant surface less residue is little to the damage of beneficial insect.Obvious to the root nematode effect.
Beauveria bassiana (B.bassiana) belongs to Deuteromycotina (Deuteromycotina), Hyphomycetes (Hyphomycetes), Moniliales (Moniliales), Moniliaceae (Moniliaceae), Beauveria (Beauveria) in classification.Beauveria bassiana is a present domestic and international application disinsection fungal comparatively widely, is considered to a kind of insect pathogenic fungus of tool potentiality to be exploited.The researcher once utilized beauveria bassiana control of maize snout moth's larva (Lewis, et al., 1996), pine caterpillars (Alves, et al., 2002), little sugarcane borer (Alves, et al., 2002), blind Chinese toon (Leland, et al., 2006), grain weevil (Vassilakos, et al., 2006), citrus red mite (Shi et al., 2006) and aphid agriculture and forestry injurious insects such as (Ye, et al., 2005).Particularly to the biological control of corn borer and pine caterpillars, China uses beauveria bassiana as conventional means year after year.Because beauveria bassiana can be controlled insect population quantity effectively, and does not injure other natural enemy insects and beneficial organism, meets the aim of integrated pest management fully, beauveria bassiana is produced in batches easily simultaneously, and cost is low, so it is with a wide range of applications.
A series of lab screening test and field experiment show, Avermectin and beauveria bassiana are compound to have significant synergies, can act on two different target points simultaneously, insect is poisoned to fall ill, particularly suitable produces the various agricultural insects such as Lepidoptera, coleoptera, Homoptera, acarina pest and root-knot nematode of resistance to the organic chemistry agricultural chemicals, to bio-safeties such as people and animals, natural enemies, good with the compatibility of environment, the biological insecticides of genus broad-spectrum high efficacy.
Embodiment
The present invention is described further below in conjunction with specific embodiment:
1, oil-suspending agent:
Embodiment 1-1: get 0.5 kilogram of Avermectin, the female powder (specification: 1,000 hundred million spores/g) 5 kilograms, 0.5 kilogram of carboxymethyl cellulose, 1.5 kilograms of MF, 15 kilograms of alkylphenol polyoxyethylene of beauveria bassiana, and supply double centner with methyl oleate, each raw material mixes after ball milling, sand milling are pulverized, disperse, and promptly gets the oil-suspending agent double centner of 0.5% Avermectin+5,000,000,000 spore/grams.
Embodiment 1-2: get 0.5 kilogram of Avermectin, the female powder (specification: 1,000 hundred million spores/g) 10 kilograms, 0.3 kilogram of sodium carboxymethylcellulose of beauveria bassiana, 3 kilograms of NNO, 5 kilograms of fatty alcohol-polyoxyethylene ether, and supply double centner with epoxidized soybean oil, each raw material is pulverized, is disperseed through ball milling, sand milling, promptly gets the oil-suspending agent double centner of 0.5% Avermectin+10,000,000,000 spore/grams.
Embodiment 1-3: get 1 kilogram of Avermectin, the female powder (specification: 1,000 hundred million spores/g) 20 kilograms, 0.4 kilogram of xanthans, 1.2 kilograms of sodium lignin sulfonates, 8 kilograms of tweens of beauveria bassiana, and supply double centner with soybean oil, each raw material is pulverized, is disperseed through ball milling, sand milling, promptly gets the oil-suspending agent double centner of 1% Avermectin+20,000,000,000 spore/grams.
Embodiment 1-4: get 10 kilograms of Avermectin, the female powder (specification: 2,000 hundred million spores/g) 20 kilograms, 0.1 kilogram of carboxymethyl cellulose, 0.5 kilogram of NNO, 6 kilograms of alkylphenol polyoxyethylene of beauveria bassiana, and supply double centner with epoxidized soybean oil, each raw material is pulverized, is disperseed through ball milling, sand milling, promptly gets the oil-suspending agent double centner of 15% Avermectin+40,000,000,000 spore/grams.
2, wetting powder
Embodiment 2-1: (specification: 1,000 hundred million spores/g) 5 kilograms, 1 kilogram of dispersant NNO, wetting agent draw back 2.5 kilograms in powder to get the female powder of 0.5 kilogram of Avermectin, beauveria bassiana, and supply double centner with bentonite, mix after each raw material pulverizing, can make 0.5% Avermectin+5,000,000,000 spores/gram wetting powder double centner.
Embodiment 2-2: get 0.5 kilogram of Avermectin, the female powder (specification: 1,000 hundred million spores/g) 10 kilograms, 7 kilograms of Dispersant MFs, 0.5 kilogram of wetting and penetrating agent T of beauveria bassiana, and supply double centner with bentonite, mix after each raw material pulverizing, can make 0.5% Avermectin+10,000,000,000 spores/gram wetting powder double centner.
Embodiment 2-3: get 1 kilogram of Avermectin, the female powder (specification: 1,000 hundred million spores/g) 20 kilograms, 10 kilograms of dispersant sodium lignin sulfonates, 3 kilograms of neopelexes of beauveria bassiana, and supply double centner with atlapulgite, mix after each raw material pulverizing, can make 1% Avermectin+20,000,000,000 spores/gram wetting powder double centner.
Embodiment 2-4: get 20 kilograms of Avermectin, the female powder (specification: 1,000 hundred million spores/g) 50 kilograms, 8 kilograms of dispersant NNO, 5 kilograms of wetting and penetrating agent T of beauveria bassiana, and supply double centner with atlapulgite, mix after each raw material pulverizing, can make 20% Avermectin+50,000,000,000 spores/gram wetting powder double centner.
Effect test
One, Toxicity Determination
1.1 assay method
Test adopts leaf dipping method to carry out.Get the fresh pine needle of capacity, in liquid medicine, soak 5S and take out, be placed on and put into culture dish after drying on the filter paper, insert then 2 age the pine caterpillars larva, at 26 ℃ ± 1 ℃; RH:80%; Single head is preserved moisture and is raised under the L/D:14h/10h condition, and each 15 of concentration processing also repeats 4 times, raises with 0.2% the fresh branches and leaves of Tween-80 aqueous solution soaking simultaneously and does blank.
The Tween-80 aqueous solution with 0.2% is made thinner, is formulated as follows test medicine:
Test medicine group 1: the single agent group of Avermectin, establish 6 concentration gradients, be followed successively by 0.5 μ g/mL, 1 μ g/mL, 2 μ g/mL, 4 μ g/mL, 8 μ g/mL, 16 μ g/mL.
Test medicine group 2: beauveria bassiana list agent group, if 6 concentration gradients, in the beauveria bassiana of 1,000 hundred million spores/g, concentration is followed successively by 50 μ g/mL (0.05 hundred million spore/mL), 100 μ g/mL (0.1 hundred million spores/mL), 200 μ g/mL (0.2 hundred million spores/mL), 400 μ g/mL (0.4 hundred million spores/mL), 800 μ g/mL (0.8 hundred million spores/mL), 1600 μ g/mL (1.6 hundred million spores/mL).
Test medicine group 3: the intermixture group, establish 6 concentration gradients, (weight ratio of 1,000 hundred million spores/g) is 1: 10 to the female powder of Avermectin and beauveria bassiana, and both total concentrations are followed successively by: 5,10,20,40,80,160 μ g/mL.
Test medicine group 4: the intermixture group, establish 6 concentration gradients, (weight ratio of 1,000 hundred million spores/g) is 1: 15 to the female powder of Avermectin and beauveria bassiana, and both total concentrations are followed successively by: 5,10,20,40,80,160 μ g/mL.
Test medicine group 5: the intermixture group, establish 6 concentration gradients, (weight ratio of 1,000 hundred million spores/g) is 1: 20 to the female powder of Avermectin and beauveria bassiana, and both total concentrations are followed successively by: 5,10,20,40,80,160 μ g/mL.
Test medicine group 6: the intermixture group, establish 6 concentration gradients, (weight ratio of 1,000 hundred million spores/g) is 1: 25 to the female powder of Avermectin and beauveria bassiana, and both total concentrations are followed successively by: 5,10,20,40,80,160 μ g/mL.
Test medicine group 7: the intermixture group, establish 6 concentration gradients, (weight ratio of 1,000 hundred million spores/g) is 1: 30 to the female powder of Avermectin and beauveria bassiana, and both total concentrations are followed successively by: 5,10,20,40,80,160 μ g/mL.
1.2 data survey and statistical analysis
Check the larva death condition behind the 4d, can not normal activity get food and be dead worm.Do regression analysis with test medicine concentration logarithm value and corresponding corrected mortality probability value, ask virulence regression equation, the LC of each medicament group
50, LC
90With 95% confidence limit, calculate the co-toxicity coefficient of Avermectin and the different proportionings of beauveria bassiana according to the abundant method of Sun Yun, the gained data see Table 1.
Table 1 Toxicity Determination result
| Test medicine | The a value | The b value | Correlation coefficient | ??LC 50?(μg/ml) | Standard error SD | ??LD 5095% confidence limit | ??LC 90?(μg/ml) | Co-toxicity coefficient |
| Medicament group 1 | ??4.3851 | ??1.4997 | ??0.9963 | ??2.5705 | ??0.0488 | ??2.0626-3.2033 | ??18.39 | |
| Medicament group 2 | ??1.2317 | ??1.5268 | ??0.9990 | ??293.8348 | ??0.0481 | ??236.5069-364.9650 | ??2030.05 | |
| Medicament group 3 | ??2.8862 | ??1.6671 | ??0.9964 | ??18.5332 | ??0.0464 | ??15.0317-22.8499 | ??108.82 | ??140.3 |
| Medicament group 4 | ??2.9660 | ??1.4846 | ??0.9990 | ??23.4459 | ??0.0495 | ??18.7500-29.3194 | ??171.13 | ??155.1 |
| Medicament group 5 | ??2.8880 | ??1.4638 | ??0.9918 | ??27.7217 | ??0.0497 | ??22.1486-34.6910 | ??208.14 | ??165.7 |
| Medicament group 6 | ??2.6151 | ??1.5347 | ??0.9978 | ??35.8084 | ??0.0483 | ??28.7944-44.5372 | ??244.94 | ??153.1 |
| Medicament group 7 | ??2.5788 | ??1.5010 | ??0.9945 | ??41.0259 | ??0.0505 | ??32.6750-51.5225 | ??293.01 | ??153.9 |
Test data by table 1 as can be seen, five groups of confections of Avermectin and beauveria bassiana all greater than 120, show significant synergistic effect to the co-toxicity coefficient of pine caterpillars.
Two, field experiment
Carry out the vermins-proof effect that following field experiment is checked biopesticide of the present invention respectively: 2008, experimental field, academy of agricultural sciences, Henan Province (base, Yuanyang), and snout moth's larva test on the control of maize tassel, experimental result sees Table 2; 2009, Zhongmou County aggie experimental field, the test of control cabbage field diamond-back moth, test effect sees Table 3; 2009, Changge institute of agricultural sciences experimental field, the test of control wheat ear aphid, the gained experimental result is listed in table 4.
Snout moth's larva result of the test on the table 2 control of maize tassel
Table 3 control cabbage field diamond-back moth result of the test
Table 4 control wheat ear aphid result of the test
Above multiple spot field demonstration test result shows, by Avermectin and beauveria bassiana composite and biological insecticides have following outstanding feature:
1, good to multiple field insect quick-acting such as common crop field Lepidoptera, Homopteras;
2, after insect was poisoned to death, beauveria bassiana was bred rapidly polypide, formed new source of infection in the field, infected repeatedly, made worm alive poisonings of catching an illness, and made medicament show the field lasting period of growing;
3, said preparation active ingredient Avermectin is the natural products that separates in the edaphon, and beauveria bassiana is the Deuteromycotina insect pathogenic fungus, all derives from biology, and environment compatibility is good, and is safe, efficient noresidue.
Claims (10)
1. the biopesticide of an Insecticiding-miticiding, its effective ingredient is Avermectin and beauveria bassiana.
2. the biopesticide of Insecticiding-miticiding according to claim 1 is characterized in that the content of beauveria bassiana is 50～50,000,000,000 spores/restraint agent in the described biopesticide, and the content of Avermectin is 0.5～20wt%.
3. as the biopesticide of Insecticiding-miticiding as described in the claim 2, it is characterized in that, described biopesticide is a wetting powder, contain beauveria bassiana 50～50,000,000,000 spores/restraint agent, Avermectin 0.5～20wt%, wetting agent 0.5～5wt%, dispersant 1～10wt%, all the other supply 100% by filler.
4. as the biopesticide of Insecticiding-miticiding as described in the claim 3, it is characterized in that described wetting agent is for drawing back powder, wetting and penetrating agent T or neopelex.
5. as the biopesticide of Insecticiding-miticiding as described in the claim 3, it is characterized in that described dispersant is NNO, MF or sodium lignin sulfonate.
6. as the biopesticide of Insecticiding-miticiding as described in the claim 3, it is characterized in that described filler is kaolin, bentonite, white carbon or atlapulgite.
7. as the biopesticide of Insecticiding-miticiding as described in the claim 2, it is characterized in that, described biopesticide is an oil-suspending agent, contain beauveria bassiana 50～40,000,000,000 spores/restraint agent, Avermectin 0.5～10wt%, thickener 0.1～0.5wt%, dispersant 0.5～3wt%, surfactant 5～15wt%, all the other supply 100% by solvent.
8. as the biopesticide of Insecticiding-miticiding as described in the claim 7, it is characterized in that described thickener is carboxymethyl cellulose, sodium carboxymethylcellulose or xanthans.
9. as the biopesticide of Insecticiding-miticiding as described in the claim 8, it is characterized in that described dispersant is NNO, MF or sodium lignin sulfonate.
10. as the biopesticide of Insecticiding-miticiding as described in the claim 9, it is characterized in that described surfactant fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene or tween; Described solvent is vegetable oil, epoxidized soybean oil or methyl oleate.
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| CN112400902A (en) * | 2020-10-22 | 2021-02-26 | 湖南顺新龙辰生物工程有限公司 | Pesticide composition |
| CN114982769A (en) * | 2022-06-04 | 2022-09-02 | 华南农业大学 | Pesticide containing emamectin benzoate and beauveria bassiana as effective components and application thereof in preventing and treating common thrips |
| CN117598324A (en) * | 2023-11-21 | 2024-02-27 | 西南大学 | Beauveria bassiana-based pesticide for controlling Bactrocera dorsalis and testing method thereof |
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