CN101851588A - Culture method for improving adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium - Google Patents
Culture method for improving adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium Download PDFInfo
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- CN101851588A CN101851588A CN200910133299A CN200910133299A CN101851588A CN 101851588 A CN101851588 A CN 101851588A CN 200910133299 A CN200910133299 A CN 200910133299A CN 200910133299 A CN200910133299 A CN 200910133299A CN 101851588 A CN101851588 A CN 101851588A
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- zygosaccharomyces rouxii
- heavy metal
- adsorption capacity
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Abstract
The invention discloses a culture method for improving adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium, which belongs to the technical field of food biology. The culture method for improving the adsorption capacity of the zygosaccharomyces rouxii heavy metal cadmium comprises the following steps of: preparing a culture medium containing 5 to 12 grams of yeast powder, 15 to 25 grams of peptone, 15 to 200 grams of glucose, 1,000mL of distilled water and 0.01 to 20 percent of sodium chloride or potassium chloride; adjusting the pH of the culture medium to be between 5.8 and 6.4, and sterilizing the culture medium for 20 minutes at the temperature of 115 DEG C; and inoculating zygosaccharomyces rouxii to the culture medium, culturing the zygosaccharomyces rouxii by liquid in a shaking bed or a fermentation tank at the temperature of between 28 and 30 DEG C to obtain zygosaccharomyces rouxii cells, and preparing freeze-drying powder. The adsorption capacity of the zygosaccharomyces rouxii dry cadmium powder is improved by 0.5 to 15 percent by adopting the method of the invention. The method of the invention is simple, good in safety and easy to implement, and has significance for clearing cadmium ions in water body, protein enzymolysis liquid, Chinese medicinal herb decoction and the like.
Description
Technical field
The present invention relates to a kind of cultural method that improves adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium, belong to technical field of food biotechnology.
Background technology
Environmental pollution causes the waters to be polluted, and causes that cadmium content exceeds standard in some food raw material, and the cadmium enrichment with food chain is in vivo threatening human health.Microorganism absorption is a very effective method that solves heavy metal pollution problem, and its application is also more and more general.
The Lu Shi yeast is fermented sauce and sauce product flavouring bacterial classification commonly used, does not still have document at present and thinks that proof Lu Shi yeast has pathogenic to the mankind.The Lu Shi yeast is easy to cultivate, and growth and breeding speed is fast, and salt tolerance is strong, still can grow in the solution of saliferous 24%, has the ability of adsorbing heavy metal in water cadmium.The present invention adopts special culture medium to prepare Lu Shi yeast dry powder, and employed substratum can obviously improve the ability of yeast to adsorbing heavy metal cadmium.
The patented method that adopts biological adsorption agent to handle industrial waste water containing Cd has at present, utilize the CN200810011100.9 and the CN200610017040.2 of beer waste yeast, utilize the CN 200610057596.4 of bacillus cereus, utilize the CN200710030649.8 of xylophyta carambola (Averrhoa carambola L.), utilize spartina alterniflora loisel's gac CN200810124261.9.CN200510031203.8 is that a kind of mushroom culture medium waste that utilizes is removed the industrial waste water containing Cd method.
Relevant Lu Shi zymic patent all be with the Lu Shi yeast as microbial starter culture, be CN200610043899.0 and CN200510045226.4 as number of patent application, the Lu Shi yeast that relates to is all as one of soy sauce and sweet fermented flour sauce flavouring starter; The Lu Shi yeast in patent CN01116463.8 and CN99124706.X also all as microbial starter culture.
Do not see at present and adopt cultural method to improve the patent of Lu Shi yeast the heavy metal cadmium adsorptive power.
Advantage of the present invention is that method is simple, need not to increase culture device, is easy to control, obviously improves the heavy metal cadmium adsorptive power.Significant to the cadmium ion that removes in the aqueous solution, taking off the cadmium processing in fields such as sewage disposal, purifying drinking water, foodstuff production and herbal medicine decocting liquid has very wide application prospect.
Summary of the invention
Technical problem to be solved by this invention is to improve yeast adsorbing heavy metal cadmium ability, can not cause new pollution to processed liquid again.
The technical process that the present invention addresses the above problem the scheme of taking is:
Step 1, with yeast extract paste 5～12g, 1. peptone 15～25g, glucose 15～200g and 0.01～20% material successively join in the 1000mL distilled water; Slowly be warming up to it and dissolve fully, obtain liquid nutrient medium, adjust medium pH to 5.8～6.4,115 ℃ sterilization 20min with hydrochloric acid and the sodium hydroxide solution of 0.1mol/L again.
Step 2 is inoculated into activatory Lu Shi yeast liquid seeds in the substratum of step 1 gained.Control shake-flask culture condition is: 25～32 ℃ of constant temperature, and shaking speed 150～200rpm, liquid amount 100ml/500ml, inoculum size 4% is cultivated 15～40h; Or control fermentor cultivation condition is: 25～32 ℃ of constant temperature, and rotating speed 150～400rpm, dress liquid coefficient 0.6, inoculum size 2～10%, ventilation 1vvm cultivates 12～50h.
Step 3 is collected thalline with the fermented liquid that step 2 obtained through the centrifugal 5～10min of 1500～6000rpm, cleans surface medium, and lyophilize is prepared into powder.
1. described material is sodium-chlor or Repone K, perhaps molasses, perhaps ammonium sulfate, perhaps sodium-chlor and potassium chloride mixture.
Described Lu Shi yeast is the Lu Shi zygosaccharomyces.
This product is white or micro-yellow powder.This powder has adsorptive power to heavy metal cadmium in the water, the Lu Shi yeast powder that the present invention obtains is put in the solution that contains heavy metal cadmium, condition is 30 ℃ of solution temperatures, pH value 5～7, absorption oscillation frequency 100rpm, processed liquid concentration of cadmium ions 50mg/L, cell concentration is 5g/L, during treatment time 30min, compare with the Lu Shi yeast powder that obtains from the YEPD substratum, powder of the present invention reaches 15% to cadmium decreasing ratio increase rate in the water.Significant to the cadmium ion that removes in the water, as the cadmium processing of taking off very wide application prospect is arranged at aspects such as sewage disposal, purifying drinking water source, protein enzyme solution, herbal medicine decocting liquid.
Embodiment
Further describe below in conjunction with embodiment.
Embodiment 1:
Step 1, with yeast extract paste 10g, peptone 18g, glucose 20g and 5% sodium-chlor successively join in the 1000mL distilled water; Slowly be warming up to it and dissolve fully, obtain liquid nutrient medium, adjust medium pH to 6.0 with hydrochloric acid and the sodium hydroxide solution of 0.1mol/L again, 115 ℃ of sterilization 20min.
Step 2 is inoculated into activatory Lu Shi yeast liquid seeds in the substratum of step 1 gained.Control shake-flask culture condition is: 28 ℃ of constant temperature, and shaking speed 180rpm, liquid amount 100ml/500ml, inoculum size 4% is cultivated 24h.
Step 3 is collected thalline with the fermented liquid that step 2 obtains through the centrifugal 20min of 4000rpm, cleans surface medium, and lyophilize is prepared into powder.
Embodiment 2:
Step 1, with yeast extract paste 10g, peptone 22g, glucose 20g and 10% Repone K successively join in the 1000mL distilled water; Slowly be warming up to it and dissolve fully, obtain liquid nutrient medium, adjust medium pH to 6.2 with hydrochloric acid and the sodium hydroxide solution of 0.1mol/L again, 115 ℃ of sterilization 20min.
Step 2 is inoculated into activatory Lu Shi yeast liquid seeds in the substratum of step 1 gained.The fermentor cultivation condition is: 28 ℃ of constant temperature, and rotating speed 300rpm, dress liquid coefficient 0.6, inoculum size 10%, ventilation 1vvm cultivates 48h.
Step 3 is collected thalline with the fermented liquid that step 2 obtains through the centrifugal 10min of 5000rpm, cleans surface medium, and lyophilize is prepared into powder.
Embodiment 3:
Step 1, with yeast extract paste 10g, peptone 20g and glucose 200g successively join in the 1000mL distilled water; Slowly be warming up to it and dissolve fully, obtain liquid nutrient medium, adjust medium pH to 6.0 with hydrochloric acid and the sodium hydroxide solution of 0.1mol/L again, 115 ℃ of sterilization 20min.
Step 2 is inoculated into activatory Lu Shi yeast liquid seeds in the substratum of step 1 gained.Control shake-flask culture condition is: 30 ℃ of constant temperature, and shaking speed 160rpm, liquid amount 100ml/500ml, inoculum size 4% is cultivated 42h.
Step 3 is collected thalline with the fermented liquid that step 2 obtains through the centrifugal 10min of 6000rpm, cleans surface medium, and lyophilize is prepared into powder.
Claims (4)
1. a cultural method that improves adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium is characterized in that, this method relates to a kind of cultural method that improves adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium.
2. the cultural method of the described raising adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium of claim 1 the steps include:
Step 1, with yeast extract paste 5～12g, 1. peptone 15～25g, glucose 15～200g and 0.01～20% material join in the 1000mL distilled water; Slowly be warming up to it and dissolve fully, obtain liquid nutrient medium, adjust medium pH to 5.8～6.4,115 ℃ sterilization 20min with hydrochloric acid and the sodium hydroxide solution of 0.1mol/L again.
Step 2 is inoculated into activatory Lu Shi yeast liquid seeds in the substratum of step 1 gained.Control shake-flask culture condition: 25～32 ℃ of constant temperature, shaking speed 150～200rpm, liquid amount 100ml/500ml, inoculum size 4% is cultivated 15～40h; Or control fermentor cultivation condition is: 25～32 ℃ of constant temperature, and rotating speed 150～400rpm, dress liquid coefficient 0.6, inoculum size 2～10%, ventilation 1vvm cultivates 12～50h.
Step 3 is collected thalline with the fermented liquid that step 2 obtains through the centrifugal 5～20min of 1500～6000rpm, and drying is prepared into powder, is used for removing of water cadmium ion.
3. the cultural method of raising adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium according to claim 2 is characterized in that, 1. the material of adding is sodium-chlor or Repone K, perhaps molasses, perhaps ammonium sulfate.
4. cultural method by each described raising adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium in the claim 1～3.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910133299A CN101851588A (en) | 2009-04-01 | 2009-04-01 | Culture method for improving adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium |
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| CN200910133299A CN101851588A (en) | 2009-04-01 | 2009-04-01 | Culture method for improving adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105621631A (en) * | 2016-03-18 | 2016-06-01 | 河海大学 | Bioremediation device and method for continuously adsorbing metal ions by adopting active yeast |
| CN107879485A (en) * | 2017-12-13 | 2018-04-06 | 合肥郑国生物科技有限公司 | A kind of biological adsorption recovery method of heavy metals in industrial wastewater |
| CN108031445A (en) * | 2017-12-13 | 2018-05-15 | 合肥郑国生物科技有限公司 | A kind of preparation method and application method of the Pichia kudriavezii adsorbent of quick adsorption heavy metal |
-
2009
- 2009-04-01 CN CN200910133299A patent/CN101851588A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| INOUHE M.,ET AL.: "Resistance to Cadmium Ions and Formation of a Cadmium-Binding Complex in Various Wild-Type Yeasts", 《PLANT CELL PHYSIOL.》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105621631A (en) * | 2016-03-18 | 2016-06-01 | 河海大学 | Bioremediation device and method for continuously adsorbing metal ions by adopting active yeast |
| CN105621631B (en) * | 2016-03-18 | 2018-03-20 | 河海大学 | Continue the biological prosthetic device and method of adsorbing metal ions using active dry yeasr |
| CN107879485A (en) * | 2017-12-13 | 2018-04-06 | 合肥郑国生物科技有限公司 | A kind of biological adsorption recovery method of heavy metals in industrial wastewater |
| CN108031445A (en) * | 2017-12-13 | 2018-05-15 | 合肥郑国生物科技有限公司 | A kind of preparation method and application method of the Pichia kudriavezii adsorbent of quick adsorption heavy metal |
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Application publication date: 20101006 |