CN101828535A - Method for breading shrimp postlarvae by biomanipulation - Google Patents
Method for breading shrimp postlarvae by biomanipulation Download PDFInfo
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- CN101828535A CN101828535A CN201010186226A CN201010186226A CN101828535A CN 101828535 A CN101828535 A CN 101828535A CN 201010186226 A CN201010186226 A CN 201010186226A CN 201010186226 A CN201010186226 A CN 201010186226A CN 101828535 A CN101828535 A CN 101828535A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for breading shrimp postlarvae by biomanipulation. The method comprises the following steps of: selecting the parent shrimp without virus WSSV and TSV, and feeding the parent shrimp with bait without virus WSSV and TSV, wherein the feeding amount per day is 4 to 6 percent of the weight and the parent shrimp is fed for four times; breading the parent shrimp under the conditions that the water temperature is between 26 and 30 DEG C, the salinity is 2.8 to 3.2 percent and the illumination intensity is 100 to 1,000 lx to make the parent shrimp reach sexual maturity; after mating, putting the female shrimp into a spawning pond to make female shrimp lay eggs, and washing and sterilizing the collected fertilized ovum; incubating the fertilized ovum into the nauplius under the conditions that the water temperature is between 28 and 31 DEG C, the salinity is 2 to 3 percent, the pH value is 7.8 to 8.6, dissolved oxygen is 6 to 10mg/l after 13 to 18 hours, putting the nauplius into a nursing pond which is used for culturing seedlings and treated by the water, wherein the density of the nauplius is 200-300 thousand/m<3>; in the late-mid period of the nauplius growing, introducing chaetoceros muelleri without virus according to the concentration of 5*104cell/ml, and when breading nauplius the into the zoea, introducing bacillus subtilis according to the concentration of 1.25*104cfu/ml; and after the shrimp is bred to 0.8 to 1cm, obtaining the seedlings. The whole breading process is subjected to regular PCR detection, and no specific pathogen WSSV and TSV is ensured in the whole breading process. The method has the advantages of no specific virus, rapid growth, high disease resistance, high stress resistance, short breeding period, large and uniform growing standard, high yield, good investment benefit and the like.
Description
Technical field
The present invention relates to a kind of specific pathogen free shrimp seed rearing method of biological regulation and control, belong to the aquatic fry growing technical field.
Background technology
Specific pathogen free animals is meant the animal that no specific virus, microorganism or parasite exists.Specific pathogen free refers to the concrete present situation of special pathogen, not at the resistance of pathogene or the situation of following pathogene.Specific pathogen free is a cause of disease control concept, rather than a kind of genotype or phenotype in the animal heredity, with the essential difference of genetics concepts such as kind, kind, mutation or strain existence of animal.The input of specific pathogen free animals will can not cause the introduction of special pathogen.At present, most of herding kinds, laboratory animal have all been set up the specific pathogen free population, and the catfish in the aquiculture animal, carp, salmon, rainbow trout, prawn etc. also have the report of specific pathogen free population.
Specific pathogen free shrimp seedling is not represented no longer to be infected by the virus, and also will depend on the quality of prawn self resistance and immunity power and environmental condition.Owing in seedling raising process, will guarantee control to causal organism, so each link all needs through disinfecting completely in the specific pathogen free shrimp seed rearing process, stop the intrusion of cause of disease, this will cause biology community structure and water quality characteristic change in the shrimp seed rearing pond, thereby influence the normal growth of shrimp seedling, reduce nonspecific cause of disease resistance.
Summary of the invention
The object of the present invention is to provide a kind of shrimp seed rearing method that improves a kind of biological regulation and control of prawn premunition, resistance.
A kind of method for breading shrimp postlarvae by biomanipulation provided by the invention is the close shrimp of selecting not carry viral WSSV and TSV, this method is the close shrimp of selecting not carry viral WSSV and TSV, throw something and feed and do not carry the bait of viral WSSV and TSV, day feeding volume is 4～6% of a body weight, throw something and feed for 4 times, under the condition of 26～30 ℃ of water temperatures, salinity 28～32 ‰ and intensity of illumination 100～10001x, cultivate and reach sexual maturity; Treat post-coitum, female shrimp put into spawning pond lay eggs that collected fertilized egg is through washing and disinfecting; Fertilized egg was hatched into nauplius at 28～31 ℃ of water temperatures, salinity 20～30 ‰, pH value 7.8～8.6, dissolved oxygen 6～10mg/l through 13～18 hours, change nauplius over to water for larval nursing nursery pond after treatment, and young density is 20～300,000/m
3, when nauplius grows to the middle and later periods, by 5 * 10
4The concentration of cell/ml is introduced and is not carried viral Chaetoceros muelleri, when growing zoea, by 1.25 * 10
4The concentration of cfu/ml is introduced bacillus subtilis, feeds and can emerge after total length reaches 0.8～1cm; All regularly carry out PCR in the above-mentioned whole seedling raising process and detect, guarantee specific pathogen free WSSV and TSV in the seedling raising process.
Selection and the mating of parent shrimp: select individuality big, stalwartness, no wound; More than the body weight ♂ shrimp 35g/ tail, more than the ♀ shrimp 40g/ tail; The prawn of not carrying white spot syndrome virus (WSSV) and Taura syndrome (TSV) is close shrimp.Parent shrimp male and female ratio is 1.5: 1.Select the ♀ shrimp that can lay eggs that night and put into the pond mating of ♂ shrimp, the ♀ shrimp of mating moves to spawning pond and lays eggs after the povidone iodine of 200PPM was sterilized 3～5 minutes.
The processing of water for larval nursing: dark precipitation, secondary sand filtration through 24 hours, it is smart that seawater adds clorox, bleaching powder or bleaching after the sand filtration, makes that available chlorine reaches 10 * 10 in the water
-6～15 * 10
-6, disinfection 12 hours adds 10 * 10 then
-6The sodium thiosulfate neutralization is removed excessive chlorine, quick-fried gas 12 hours.
The biology of growing seedlings regulation and control: introduce Chaetoceros muelleri and bacillus subtilis, Chaetoceros muelleri and bacillus subtilis bacteria concentration in the regular check every day nursery pond, as do not reach and require concentration, need respectively to replenish and add, keep 5 * 10
4The Chaetoceros muelleri of cell/ml concentration and 1.25 * 10
4The bacillus subtilis of cfu/ml concentration is to reach the effect of biological regulation and control.
The flushing of fertilized egg and disinfecting: be earlier fertilized egg to be put into 300 order mesh bags flushing 3 minutes, put into the Iodophor 10～15ppm of amount of iodine 10% then, sterilized 2～4 minutes.
Disinfecting of bait: the bait of close shrimp clam worm alive and squid must not carried WSSV and TSV virus by specially treated and by detecting; The bait Chaetoceros muelleri algae kind of shrimp seedling must be the aseptic little algae through technical finesse, cultivates algae kind water and also need pass through strict sterilization, and algae liquid also needs to carry out the virus detection before going into the pond, detects qualified can the use.
The present invention has following advantage: the inventor is by research, integrated treatment by water quality, stop entering of specific pathogen, introducing in the system of growing seedlings does not have the bacterium frond system that harm Chaetoceros muelleri one bacillus subtilis of causing a disease is formed to human and breed object, and inhibition causal organism (vibrios) growth reduces ammonia nitrogen and nitrite nitrogen, improve water quality, improve shrimp seedling immunity related enzyme activity (AKP, SOD, POD, PO etc.), strengthen shrimp seedling premunition, promote to culture the object growth.The present invention have do not contain specific virus, growth rapidly, short, the forming specification of strong, the strong stress resistance of premunition, culture-cycle greatly and evenly, advantages such as output is high, and returns of investment are good.
Embodiment
Embodiment:
1, the processing of water for larval nursing: water for larval nursing should dark precipitation, secondary sand filtration through 24 hours.The one-level sand filtration is that 0.2～0.3 centimetre coarse sands layer filters through thick 50 centimetres, particle size; 12 cubic metres of secondary sand filter volumes, sand filter bottommost must stay fundamental space about 10 centimetres and sieve plate as support; Cobble one deck that sieve plate upper berth diameter is 4 centimetres; Rubble upper berth 50 cm thicks, particle size are 0.1～0.2 centimetre coarse sand; 25 centimetres on rough sand upper berth, particle diameter are at the fine sand below 0.1 centimetre.Husky filter back seawater also need add clorox, makes that available chlorine reaches 12.5 * 10 in the water
-6, disinfection 9 hours adds 10 * 10 then
-6Excessive chlorine is removed in the sodium thiosulfate neutralization, and fully sudden and violent gas used after 12 hours.
2, the selection of close shrimp, cultivate and breed: select individual big, stalwartness, no wound; More than the body weight ♂ shrimp 35g/ tail, more than the ♀ shrimp 40g/ tail; The prawn of not carrying WSSV and TSV is close shrimp.Parent shrimp male and female ratio is 1.5: 1.
Parent shrimp rearing is thrown something and fed and is not carried the bait of viral WSSV and TSV, and bait is made up of live clam worm and Fresh squid, and a day feeding volume is 5% of a close shrimp body weight, divides and throws something and feeds for 4 times.Under the condition of 28 ℃ of water temperatures, salinity 30 ‰ and intensity of illumination 6001x, carry out reinforced cultivating and reach sexual maturity.Breeding of shrimp of parent is to put into the pond mating of ♂ shrimp with reach sexually matured ♀ shrimp through cultivation, and the ♀ shrimp of mating moves to spawning pond and lays eggs after the povidone iodine of 200PPM was sterilized 4 minutes.Fertilized egg will be washed and disinfect, and fertilized egg was hatched into nauplius at 29 ℃ of water temperatures, salinity 25 ‰, pH value 8.2, dissolved oxygen 8mg/l through 15 hours.
3, the cultivation of shrimp seedling: change nauplius over to nursery pond, young density is 250,000/m
3, when nauplius grows to the middle and later periods, by 5 * 10
4The concentration of cell/ml is introduced Chaetoceros muelleri.When growing zoea, by 1.25 * 10
4The concentration of cfu/ml is introduced bacillus subtilis.Make regular check on Chaetoceros muelleri and bacillus subtilis bacteria concentration in the nursery pond every day, as do not reach and require concentration, replenish interpolation.And the artitificial foods such as prawn slice, BP, particulate son and spirulina of throwing something and feeding by 2 gram/skies, ten thousand tails, divide and throw something and feed for 6 times, threw something and fed continuously 7～8 days, up to the mysis later stage, from the mysis later stage capacity fairy shrimp that begins to throw something and feed, during cultivation to 16 day, shrimp seedling total length reaches the qualified after testing sale of can emerging behind 0.8～1.2cm.。
All regularly carry out PCR in the above-mentioned whole seedling raising process and detect, guarantee specific pathogen free WSSV and TSV in the seedling raising process.
4, sell in the pond that goes out of shrimp seedling: prawn is fed after total length reaches 0.8～1cm, can sell.Before sales carries out PCR at specific virus and detects, and detects prawn immunity related enzyme activity (AKP, SOD, POD, PO etc.) simultaneously, and the premunition that guarantees to emerge by force, is not with specific pathogen.
Claims (4)
1. method for breading shrimp postlarvae by biomanipulation, it is characterized in that this method is the close shrimp of selecting not carry viral WSSV and TSV, throw something and feed and do not carry the bait of viral WSSV and TSV, day feeding volume is 4～6% of a body weight, throw something and feed for 4 times, under the condition of 26～30 ℃ of water temperatures, salinity 28～32 ‰ and intensity of illumination 100～1000lx, cultivate and reach sexual maturity; Treat post-coitum, female shrimp put into spawning pond lay eggs that collected fertilized egg is through washing and disinfecting; Fertilized egg was hatched into nauplius at 28～31 ℃ of water temperatures, salinity 20～30 ‰, pH value 7.8～8.6, dissolved oxygen 6～10mg/l through 13～18 hours, change nauplius over to water for larval nursing nursery pond after treatment, and young density is 20～300,000/m
3, when nauplius grows to the middle and later periods, by 5 * 10
4The concentration of cell/ml is introduced and is not carried viral Chaetoceros muelleri, when growing zoea, by 1.25 * 10
4The concentration of cfu/ml is introduced bacillus subtilis, feeds and can emerge after total length reaches 0.8～1cm; All regularly carry out PCR in the above-mentioned whole seedling raising process and detect, guarantee specific pathogen free WSSV and TSV in the seedling raising process.
2. a kind of method for breading shrimp postlarvae by biomanipulation according to claim 1, the processing that it is characterized in that water for larval nursing are dark precipitation, secondary sand filtration through 24 hours, and it is smart that seawater adds clorox, bleaching powder or bleaching after the sand filtration, make that available chlorine reaches 10 * 10 in the water
-6～15 * 10
-6, disinfection 12 hours adds 10 * 10 then
-6The sodium thiosulfate neutralization is removed excessive chlorine, quick-fried gas 12 hours.
3. a kind of method for breading shrimp postlarvae by biomanipulation according to claim 1, it is characterized in that when introducing Chaetoceros muelleri and bacillus subtilis, Chaetoceros muelleri and bacillus subtilis bacteria concentration in the regular check every day nursery pond, as do not reach and require concentration, need respectively to replenish and add.
4. a kind of method for breading shrimp postlarvae by biomanipulation according to claim 1, it is characterized in that the fertilized egg flushing and disinfecting is earlier fertilized egg to be put into 300 order mesh bags flushing 3 minutes, puts into the Iodophor 10～15ppm of amount of iodine 10% then, sterilizes 2～4 minutes.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102187837A (en) * | 2011-05-17 | 2011-09-21 | 高雷 | Industrially breeding method of macrobrachium nipponense |
| CN102823525A (en) * | 2012-08-21 | 2012-12-19 | 江苏省海洋水产研究所 | Ecological healthy culture method of Palaemon carinicauda |
| CN104431524A (en) * | 2014-12-10 | 2015-03-25 | 苏州埃瑞特生物技术有限公司 | Research and development of special protective liquid preparation for hatching penaeus vannamei boone |
| CN105994042A (en) * | 2016-05-30 | 2016-10-12 | 铜陵东晟生态农业科技有限公司 | Prawn breeding method |
| CN110150195A (en) * | 2019-03-29 | 2019-08-23 | 天津市水产研究所 | A kind of incubation method of the litopenaeus vannamei young |
| CN110679524A (en) * | 2019-09-19 | 2020-01-14 | 海南海壹水产种苗有限公司 | Indoor industrialized ecological specific pathogen-free seedling cultivation method for litopenaeus vannamei |
| CN112655622A (en) * | 2020-12-24 | 2021-04-16 | 中国水产科学研究院淡水渔业研究中心 | Production process of crayfish SPF (specific pathogen free) seedlings |
| CN112690237A (en) * | 2020-12-27 | 2021-04-23 | 海南上泰水产种苗养殖有限公司 | Industrial ecological breeding method of shrimp larvae with high stress resistance and disease resistance |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102187837A (en) * | 2011-05-17 | 2011-09-21 | 高雷 | Industrially breeding method of macrobrachium nipponense |
| CN102187837B (en) * | 2011-05-17 | 2014-08-20 | 高雷 | Industrially breeding method of macrobrachium nipponense |
| CN102823525A (en) * | 2012-08-21 | 2012-12-19 | 江苏省海洋水产研究所 | Ecological healthy culture method of Palaemon carinicauda |
| CN102823525B (en) * | 2012-08-21 | 2014-05-28 | 江苏省海洋水产研究所 | Ecological healthy culture method of Palaemon carinicauda |
| CN104431524A (en) * | 2014-12-10 | 2015-03-25 | 苏州埃瑞特生物技术有限公司 | Research and development of special protective liquid preparation for hatching penaeus vannamei boone |
| CN105994042A (en) * | 2016-05-30 | 2016-10-12 | 铜陵东晟生态农业科技有限公司 | Prawn breeding method |
| CN110150195A (en) * | 2019-03-29 | 2019-08-23 | 天津市水产研究所 | A kind of incubation method of the litopenaeus vannamei young |
| CN110150195B (en) * | 2019-03-29 | 2021-10-01 | 天津市水产研究所 | Incubation method of litopenaeus vannamei larvae |
| CN110679524A (en) * | 2019-09-19 | 2020-01-14 | 海南海壹水产种苗有限公司 | Indoor industrialized ecological specific pathogen-free seedling cultivation method for litopenaeus vannamei |
| CN112655622A (en) * | 2020-12-24 | 2021-04-16 | 中国水产科学研究院淡水渔业研究中心 | Production process of crayfish SPF (specific pathogen free) seedlings |
| CN112690237A (en) * | 2020-12-27 | 2021-04-23 | 海南上泰水产种苗养殖有限公司 | Industrial ecological breeding method of shrimp larvae with high stress resistance and disease resistance |
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Open date: 20100915 |